TY  - JOUR
AU  - Manojlović-Stojanoski, Milica
AU  - Borković Mitić, Slavica
AU  - Nestorović, Nataša
AU  - Ristić, Nataša
AU  - Trifunović, Svetlana
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Stojsavljević, Aleksandar
AU  - Pavlović, Slađan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5198
AB  - The chemical element selenium (Se) is a nonmetal that is in trace amounts indispensable for normal cellular functioning. During pregnancy a low Se status can increase the risk of oxidative stress. However, elevated concentrations of Se in the body can also cause oxidative stress. This study aimed to compare the effects of BSA-stabilized Se nanoparticles (SeNPs, Se0) and inorganic sodium-selenite (NaSe, Se+4) supplementation on the histological structure of the placenta, oxidative stress parameters and the total placental Se concentration of Wistar rats during pregnancy.  Pregnant females were randomized into four groups: (i) intact controls; (ii) controls that were dosed by daily oral gavage with 8.6% bovine serum albumin (BSA) and 0.125 M vit C; (iii) the SeNP group that was administered 0.5 mg of SeNPs stabilized with 8.6% BSA and 0.125 M vit C/kg bw/day by oral gavage dosing; (iv) the NaSe group, gavage dosed with 0.5 mg Na2SeO3/kg bw/day. The treatment of pregnant females  started on gestational day one, lasted until day 20, and on day 21 of gestation, the fetuses with the placenta were removed from the uterus. Our findings show that the mode of action of equivalent concentrations of Se in SeNPs and NaSe depended on its redox state and chemical structure. Administration of SeNPs (Se0) increased fetal lethality and induced changes in the antioxidative defense parameters in the placenta. The accumulation of Se in the placenta was highest in SeNP-treated animals. All obtained data indicate an increased bioavailability of Se in its organic nano form and Se0 redox state in comparison to its inorganic sodium selenite form and Se+4 redox state
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta
IS  - 21
VL  - 23
DO  - 10.3390/ijms232113068
SP  - 13068
ER  - 

@article{
author = "Manojlović-Stojanoski, Milica and Borković Mitić, Slavica and Nestorović, Nataša and Ristić, Nataša and Trifunović, Svetlana and Stevanović, Magdalena and Filipović, Nenad and Stojsavljević, Aleksandar and Pavlović, Slađan",
year = "2022",
abstract = "The chemical element selenium (Se) is a nonmetal that is in trace amounts indispensable for normal cellular functioning. During pregnancy a low Se status can increase the risk of oxidative stress. However, elevated concentrations of Se in the body can also cause oxidative stress. This study aimed to compare the effects of BSA-stabilized Se nanoparticles (SeNPs, Se0) and inorganic sodium-selenite (NaSe, Se+4) supplementation on the histological structure of the placenta, oxidative stress parameters and the total placental Se concentration of Wistar rats during pregnancy.  Pregnant females were randomized into four groups: (i) intact controls; (ii) controls that were dosed by daily oral gavage with 8.6% bovine serum albumin (BSA) and 0.125 M vit C; (iii) the SeNP group that was administered 0.5 mg of SeNPs stabilized with 8.6% BSA and 0.125 M vit C/kg bw/day by oral gavage dosing; (iv) the NaSe group, gavage dosed with 0.5 mg Na2SeO3/kg bw/day. The treatment of pregnant females  started on gestational day one, lasted until day 20, and on day 21 of gestation, the fetuses with the placenta were removed from the uterus. Our findings show that the mode of action of equivalent concentrations of Se in SeNPs and NaSe depended on its redox state and chemical structure. Administration of SeNPs (Se0) increased fetal lethality and induced changes in the antioxidative defense parameters in the placenta. The accumulation of Se in the placenta was highest in SeNP-treated animals. All obtained data indicate an increased bioavailability of Se in its organic nano form and Se0 redox state in comparison to its inorganic sodium selenite form and Se+4 redox state",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta",
number = "21",
volume = "23",
doi = "10.3390/ijms232113068",
pages = "13068"
}

Manojlović-Stojanoski, M., Borković Mitić, S., Nestorović, N., Ristić, N., Trifunović, S., Stevanović, M., Filipović, N., Stojsavljević, A.,& Pavlović, S.. (2022). The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta. in International Journal of Molecular Sciences
Basel: MDPI., 23(21), 13068.
https://doi.org/10.3390/ijms232113068

Manojlović-Stojanoski M, Borković Mitić S, Nestorović N, Ristić N, Trifunović S, Stevanović M, Filipović N, Stojsavljević A, Pavlović S. The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta. in International Journal of Molecular Sciences. 2022;23(21):13068.
doi:10.3390/ijms232113068 .

Manojlović-Stojanoski, Milica, Borković Mitić, Slavica, Nestorović, Nataša, Ristić, Nataša, Trifunović, Svetlana, Stevanović, Magdalena, Filipović, Nenad, Stojsavljević, Aleksandar, Pavlović, Slađan, "The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta" in International Journal of Molecular Sciences, 23, no. 21 (2022):13068,
https://doi.org/10.3390/ijms232113068 . .

TY  - JOUR
AU  - Nikolić, Sandra
AU  - Gazdić-Janković, Marina
AU  - Rosić, Gvozden
AU  - Miletić-Kovačević, Marina
AU  - Jovičić, Nemanja
AU  - Nestorović, Nataša
AU  - Stojković, Petra
AU  - Filipović, Nenad
AU  - Milošević-Đorđević, Olivera
AU  - Selaković, Dragica
AU  - Živanović, Marko
AU  - Seklić, Dragana
AU  - Milivojević, Nevena
AU  - Marković, Aleksandra
AU  - Seist, Richard
AU  - Vasilijić, Sasa
AU  - Stanković, Konstantina M.
AU  - Stojković, Miodrag
AU  - Ljujić, Biljana
PY  - 2022
UR  - http://www.ncbi.nlm.nih.gov/pubmed/35405220
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4950
AB  - Commercially manufactured or generated through environmental degradation, microplastics (MPs) and nanoplastics (NPs) considerably contribute to environmental pollution. There is a knowledge gap in how exposure to MPs/NPs changes cellular function and affects animal and human health. Here, we demonstrate that after oral uptake, fluorescent polystyrene (PS) nanoparticles pass through the mouse digestive system, accumulate and aggregate in different organs, and induce functional changes in cells and organs. Using cochlear explant as a novel in vitro system, we confirmed the consequences of PS-MP/NP interaction with inner ear cells by detecting aggregates and hetero-aggregates of PS particles in hair cells. The testes of treated males accumulated MPs/NPs in the interstitial compartment surrounding the seminiferous tubules, which was associated with a statistically significant decrease in testosterone levels. Male mice showed increased secretion of interleukins (IL-12p35 and IL-23) by splenocytes while cyto- and genotoxicity tests indicated impaired cell viability and increased DNA damage in spleen tissue. Males also showed a broad range of anxiogenic responses to PS nanoparticles while hippocampal samples from treated females showed an increased expression of Bax and Nlrp3 genes, indicating a pro-apoptotic/proinflammatory effect of PS treatment. Taken together, induced PS effects are also gender-dependent, and therefore, strongly motivate future research to mitigate the deleterious effects of nanosized plastic particles.
PB  - Elsevier Ltd
T2  - Environmental Pollution
T1  - Orally administered fluorescent nanosized polystyrene particles affect cell viability, hormonal and inflammatory profile, and behavior in treated mice.
VL  - 305
DO  - 10.1016/j.envpol.2022.119206
SP  - 119206
ER  - 

@article{
author = "Nikolić, Sandra and Gazdić-Janković, Marina and Rosić, Gvozden and Miletić-Kovačević, Marina and Jovičić, Nemanja and Nestorović, Nataša and Stojković, Petra and Filipović, Nenad and Milošević-Đorđević, Olivera and Selaković, Dragica and Živanović, Marko and Seklić, Dragana and Milivojević, Nevena and Marković, Aleksandra and Seist, Richard and Vasilijić, Sasa and Stanković, Konstantina M. and Stojković, Miodrag and Ljujić, Biljana",
year = "2022",
abstract = "Commercially manufactured or generated through environmental degradation, microplastics (MPs) and nanoplastics (NPs) considerably contribute to environmental pollution. There is a knowledge gap in how exposure to MPs/NPs changes cellular function and affects animal and human health. Here, we demonstrate that after oral uptake, fluorescent polystyrene (PS) nanoparticles pass through the mouse digestive system, accumulate and aggregate in different organs, and induce functional changes in cells and organs. Using cochlear explant as a novel in vitro system, we confirmed the consequences of PS-MP/NP interaction with inner ear cells by detecting aggregates and hetero-aggregates of PS particles in hair cells. The testes of treated males accumulated MPs/NPs in the interstitial compartment surrounding the seminiferous tubules, which was associated with a statistically significant decrease in testosterone levels. Male mice showed increased secretion of interleukins (IL-12p35 and IL-23) by splenocytes while cyto- and genotoxicity tests indicated impaired cell viability and increased DNA damage in spleen tissue. Males also showed a broad range of anxiogenic responses to PS nanoparticles while hippocampal samples from treated females showed an increased expression of Bax and Nlrp3 genes, indicating a pro-apoptotic/proinflammatory effect of PS treatment. Taken together, induced PS effects are also gender-dependent, and therefore, strongly motivate future research to mitigate the deleterious effects of nanosized plastic particles.",
publisher = "Elsevier Ltd",
journal = "Environmental Pollution",
title = "Orally administered fluorescent nanosized polystyrene particles affect cell viability, hormonal and inflammatory profile, and behavior in treated mice.",
volume = "305",
doi = "10.1016/j.envpol.2022.119206",
pages = "119206"
}

Nikolić, S., Gazdić-Janković, M., Rosić, G., Miletić-Kovačević, M., Jovičić, N., Nestorović, N., Stojković, P., Filipović, N., Milošević-Đorđević, O., Selaković, D., Živanović, M., Seklić, D., Milivojević, N., Marković, A., Seist, R., Vasilijić, S., Stanković, K. M., Stojković, M.,& Ljujić, B.. (2022). Orally administered fluorescent nanosized polystyrene particles affect cell viability, hormonal and inflammatory profile, and behavior in treated mice.. in Environmental Pollution
Elsevier Ltd., 305, 119206.
https://doi.org/10.1016/j.envpol.2022.119206

Nikolić S, Gazdić-Janković M, Rosić G, Miletić-Kovačević M, Jovičić N, Nestorović N, Stojković P, Filipović N, Milošević-Đorđević O, Selaković D, Živanović M, Seklić D, Milivojević N, Marković A, Seist R, Vasilijić S, Stanković KM, Stojković M, Ljujić B. Orally administered fluorescent nanosized polystyrene particles affect cell viability, hormonal and inflammatory profile, and behavior in treated mice.. in Environmental Pollution. 2022;305:119206.
doi:10.1016/j.envpol.2022.119206 .

Nikolić, Sandra, Gazdić-Janković, Marina, Rosić, Gvozden, Miletić-Kovačević, Marina, Jovičić, Nemanja, Nestorović, Nataša, Stojković, Petra, Filipović, Nenad, Milošević-Đorđević, Olivera, Selaković, Dragica, Živanović, Marko, Seklić, Dragana, Milivojević, Nevena, Marković, Aleksandra, Seist, Richard, Vasilijić, Sasa, Stanković, Konstantina M., Stojković, Miodrag, Ljujić, Biljana, "Orally administered fluorescent nanosized polystyrene particles affect cell viability, hormonal and inflammatory profile, and behavior in treated mice." in Environmental Pollution, 305 (2022):119206,
https://doi.org/10.1016/j.envpol.2022.119206 . .

TY  - JOUR
AU  - Avdović, Edina H.
AU  - Petrović, Isidora P.
AU  - Stevanović, Milena J.
AU  - Saso, Luciano
AU  - Dimitrić Marković, Jasmina M.
AU  - Filipović, Nenad D.
AU  - Živić, Miroslav Ž.
AU  - Cvetić Antić, Tijana N.
AU  - Žižić, Milan V.
AU  - Todorović, Nataša
AU  - Vukić, Milena
AU  - Trifunović, Srećko R.
AU  - Marković, Zoran S.
PY  - 2021
UR  - https://www.hindawi.com/journals/omcl/2021/8849568/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4244
AB  - Two newly synthesized 4-hydroxycoumarin bidentate ligands (L1 and L2) and their palladium(II) complexes (C1 and C2) were screened for their biological activities, in vitro and in vivo. Structures of new compounds were established based on elemental analysis, 1H NMR, 13C NMR, and IR spectroscopic techniques. The obtained compounds were tested for their antioxidative and cytotoxic activities and results pointed to selective antiradical activity of palladium(II) complexes towards •OH and -•OOH radicals and anti-ABTS (2,2 ′ -Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical) activity comparable to that of ascorbate. Results indicated the effect of C1 and C2 on the enzymatic activity of the antioxidative defense system. In vitro cytotoxicity assay performed on different carcinoma cell lines (HCT166, A375, and MIA PaCa-2), and one healthy fibroblast cell line (MRC-5) showed a cytotoxic effect of both C1 and C2, expressed as a decrease in carcinoma cells’ viability, mostly by induction of apoptosis. In vivo toxicity tests performed on zebrafish embryos indicated different effects of C1 and C2, ranging from adverse developmental effect to no toxicity, depending on tested concentration. According to docking studies, both complexes (C1 and C2) showed better inhibitory activity in comparison to other palladium(II) complexes.
PB  - Hindawi Limited
T2  - Oxidative Medicine and Cellular Longevity
T1  - Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes
VL  - 2021
DO  - 10.1155/2021/8849568
SP  - 8849568
ER  - 

@article{
author = "Avdović, Edina H. and Petrović, Isidora P. and Stevanović, Milena J. and Saso, Luciano and Dimitrić Marković, Jasmina M. and Filipović, Nenad D. and Živić, Miroslav Ž. and Cvetić Antić, Tijana N. and Žižić, Milan V. and Todorović, Nataša and Vukić, Milena and Trifunović, Srećko R. and Marković, Zoran S.",
year = "2021",
abstract = "Two newly synthesized 4-hydroxycoumarin bidentate ligands (L1 and L2) and their palladium(II) complexes (C1 and C2) were screened for their biological activities, in vitro and in vivo. Structures of new compounds were established based on elemental analysis, 1H NMR, 13C NMR, and IR spectroscopic techniques. The obtained compounds were tested for their antioxidative and cytotoxic activities and results pointed to selective antiradical activity of palladium(II) complexes towards •OH and -•OOH radicals and anti-ABTS (2,2 ′ -Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical) activity comparable to that of ascorbate. Results indicated the effect of C1 and C2 on the enzymatic activity of the antioxidative defense system. In vitro cytotoxicity assay performed on different carcinoma cell lines (HCT166, A375, and MIA PaCa-2), and one healthy fibroblast cell line (MRC-5) showed a cytotoxic effect of both C1 and C2, expressed as a decrease in carcinoma cells’ viability, mostly by induction of apoptosis. In vivo toxicity tests performed on zebrafish embryos indicated different effects of C1 and C2, ranging from adverse developmental effect to no toxicity, depending on tested concentration. According to docking studies, both complexes (C1 and C2) showed better inhibitory activity in comparison to other palladium(II) complexes.",
publisher = "Hindawi Limited",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes",
volume = "2021",
doi = "10.1155/2021/8849568",
pages = "8849568"
}

Avdović, E. H., Petrović, I. P., Stevanović, M. J., Saso, L., Dimitrić Marković, J. M., Filipović, N. D., Živić, M. Ž., Cvetić Antić, T. N., Žižić, M. V., Todorović, N., Vukić, M., Trifunović, S. R.,& Marković, Z. S.. (2021). Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes. in Oxidative Medicine and Cellular Longevity
Hindawi Limited., 2021, 8849568.
https://doi.org/10.1155/2021/8849568

Avdović EH, Petrović IP, Stevanović MJ, Saso L, Dimitrić Marković JM, Filipović ND, Živić MŽ, Cvetić Antić TN, Žižić MV, Todorović N, Vukić M, Trifunović SR, Marković ZS. Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes. in Oxidative Medicine and Cellular Longevity. 2021;2021:8849568.
doi:10.1155/2021/8849568 .

Avdović, Edina H., Petrović, Isidora P., Stevanović, Milena J., Saso, Luciano, Dimitrić Marković, Jasmina M., Filipović, Nenad D., Živić, Miroslav Ž., Cvetić Antić, Tijana N., Žižić, Milan V., Todorović, Nataša, Vukić, Milena, Trifunović, Srećko R., Marković, Zoran S., "Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes" in Oxidative Medicine and Cellular Longevity, 2021 (2021):8849568,
https://doi.org/10.1155/2021/8849568 . .

TY  - JOUR
AU  - Valente, Andreia
AU  - Podolski-Renić, Ana
AU  - Poetsch, Isabella
AU  - Filipović, Nenad
AU  - López, Óscar
AU  - Turel, Iztok
AU  - Heffeter, Petra
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1368764621000364
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4463
AB  - Drug resistance remains the major cause of cancer treatment failure especially at the late stage of the disease. However, based on their versatile chemistry, metal and metalloid compounds offer the possibility to design fine-tuned drugs to circumvent and even specifically target drug-resistant cancer cells. Based on the paramount importance of platinum drugs in the clinics, two main areas of drug resistance reversal strategies exist: overcoming resistance to platinum drugs as well as multidrug resistance based on ABC efflux pumps. The current review provides an overview of both aspects of drug design and discusses the open questions in the field. The areas of drug resistance covered in this article involve: 1) Altered expression of proteins involved in metal uptake, efflux or intracellular distribution, 2) Enhanced drug efflux via ABC transporters, 3) Altered metabolism in drug-resistant cancer cells, 4) Altered thiol or redox homeostasis, 5) Altered DNA damage recognition and enhanced DNA damage repair, 6) Impaired induction of apoptosis and 7) Altered interaction with the immune system. This review represents the first collection of metal (including platinum, ruthenium, iridium, gold, and copper) and metalloid drugs (e.g. arsenic and selenium) which demonstrated drug resistance reversal activity. A special focus is on compounds characterized by collateral sensitivity of ABC transporter-overexpressing cancer cells. Through this approach, we wish to draw the attention to open research questions in the field. Future investigations are warranted to obtain more insights into the mechanisms of action of the most potent compounds which target specific modalities of drug resistance.
PB  - Edinburgh: Churchill Livingstone
T2  - Drug Resistance Updates
T1  - Metal- and metalloid-based compounds to target and reverse cancer multidrug resistance.
VL  - 58
DO  - 10.1016/j.drup.2021.100778
SP  - 100778
ER  - 

@article{
author = "Valente, Andreia and Podolski-Renić, Ana and Poetsch, Isabella and Filipović, Nenad and López, Óscar and Turel, Iztok and Heffeter, Petra",
year = "2021",
abstract = "Drug resistance remains the major cause of cancer treatment failure especially at the late stage of the disease. However, based on their versatile chemistry, metal and metalloid compounds offer the possibility to design fine-tuned drugs to circumvent and even specifically target drug-resistant cancer cells. Based on the paramount importance of platinum drugs in the clinics, two main areas of drug resistance reversal strategies exist: overcoming resistance to platinum drugs as well as multidrug resistance based on ABC efflux pumps. The current review provides an overview of both aspects of drug design and discusses the open questions in the field. The areas of drug resistance covered in this article involve: 1) Altered expression of proteins involved in metal uptake, efflux or intracellular distribution, 2) Enhanced drug efflux via ABC transporters, 3) Altered metabolism in drug-resistant cancer cells, 4) Altered thiol or redox homeostasis, 5) Altered DNA damage recognition and enhanced DNA damage repair, 6) Impaired induction of apoptosis and 7) Altered interaction with the immune system. This review represents the first collection of metal (including platinum, ruthenium, iridium, gold, and copper) and metalloid drugs (e.g. arsenic and selenium) which demonstrated drug resistance reversal activity. A special focus is on compounds characterized by collateral sensitivity of ABC transporter-overexpressing cancer cells. Through this approach, we wish to draw the attention to open research questions in the field. Future investigations are warranted to obtain more insights into the mechanisms of action of the most potent compounds which target specific modalities of drug resistance.",
publisher = "Edinburgh: Churchill Livingstone",
journal = "Drug Resistance Updates",
title = "Metal- and metalloid-based compounds to target and reverse cancer multidrug resistance.",
volume = "58",
doi = "10.1016/j.drup.2021.100778",
pages = "100778"
}

Valente, A., Podolski-Renić, A., Poetsch, I., Filipović, N., López, Ó., Turel, I.,& Heffeter, P.. (2021). Metal- and metalloid-based compounds to target and reverse cancer multidrug resistance.. in Drug Resistance Updates
Edinburgh: Churchill Livingstone., 58, 100778.
https://doi.org/10.1016/j.drup.2021.100778

Valente A, Podolski-Renić A, Poetsch I, Filipović N, López Ó, Turel I, Heffeter P. Metal- and metalloid-based compounds to target and reverse cancer multidrug resistance.. in Drug Resistance Updates. 2021;58:100778.
doi:10.1016/j.drup.2021.100778 .

Valente, Andreia, Podolski-Renić, Ana, Poetsch, Isabella, Filipović, Nenad, López, Óscar, Turel, Iztok, Heffeter, Petra, "Metal- and metalloid-based compounds to target and reverse cancer multidrug resistance." in Drug Resistance Updates, 58 (2021):100778,
https://doi.org/10.1016/j.drup.2021.100778 . .