TY  - JOUR
AU  - Stupar, Petar
AU  - Podolski-Renić, Ana
AU  - Villalba, Maria Ines
AU  - Dragoj, Miodrag
AU  - Jovanović Stojanov, Sofija
AU  - Pešić, Milica
AU  - Kasas, Sandor
PY  - 2021
UR  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147836/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4247
AB  - Background and Objectives: Optimization of chemotherapy is crucial for cancer patients. Timely and costly efficient treatments are emerging due to the increasing incidence of cancer world-wide. Here, we present a methodology of nano-motion analysis that could be developed to serve as a screening tool able to determine the best chemotherapy option for a particular patient within hours. Materials and Methods: Three different human cancer cell lines and their multidrug resistant (MDR) counterparts were analyzed with an atomic force microscope (AFM) using tipless cantilevers to adhere the cells and monitor their nano-motions. Results: The cells exposed to doxorubicin (DOX) differentially responded due to their sensitivity to this chemotherapeutic. The death of sensitive cells corresponding to the drop in signal variance occurred in less than 2 h after DOX application, while MDR cells continued to move, even showing an increase in signal variance. Conclusions: Nano-motion sensing can be developed as a screening tool that will allow simple, inexpensive and quick testing of different chemotherapeutics for each cancer patient. Further investigations on patient-derived tumor cells should confirm the method’s applicability.
PB  - MDPI AG
T2  - Medicina
T1  - Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics
IS  - 5
VL  - 57
DO  - 10.3390/medicina57050446
SP  - 446
ER  - 

@article{
author = "Stupar, Petar and Podolski-Renić, Ana and Villalba, Maria Ines and Dragoj, Miodrag and Jovanović Stojanov, Sofija and Pešić, Milica and Kasas, Sandor",
year = "2021",
abstract = "Background and Objectives: Optimization of chemotherapy is crucial for cancer patients. Timely and costly efficient treatments are emerging due to the increasing incidence of cancer world-wide. Here, we present a methodology of nano-motion analysis that could be developed to serve as a screening tool able to determine the best chemotherapy option for a particular patient within hours. Materials and Methods: Three different human cancer cell lines and their multidrug resistant (MDR) counterparts were analyzed with an atomic force microscope (AFM) using tipless cantilevers to adhere the cells and monitor their nano-motions. Results: The cells exposed to doxorubicin (DOX) differentially responded due to their sensitivity to this chemotherapeutic. The death of sensitive cells corresponding to the drop in signal variance occurred in less than 2 h after DOX application, while MDR cells continued to move, even showing an increase in signal variance. Conclusions: Nano-motion sensing can be developed as a screening tool that will allow simple, inexpensive and quick testing of different chemotherapeutics for each cancer patient. Further investigations on patient-derived tumor cells should confirm the method’s applicability.",
publisher = "MDPI AG",
journal = "Medicina",
title = "Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics",
number = "5",
volume = "57",
doi = "10.3390/medicina57050446",
pages = "446"
}

Stupar, P., Podolski-Renić, A., Villalba, M. I., Dragoj, M., Jovanović Stojanov, S., Pešić, M.,& Kasas, S.. (2021). Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics. in Medicina
MDPI AG., 57(5), 446.
https://doi.org/10.3390/medicina57050446

Stupar P, Podolski-Renić A, Villalba MI, Dragoj M, Jovanović Stojanov S, Pešić M, Kasas S. Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics. in Medicina. 2021;57(5):446.
doi:10.3390/medicina57050446 .

Stupar, Petar, Podolski-Renić, Ana, Villalba, Maria Ines, Dragoj, Miodrag, Jovanović Stojanov, Sofija, Pešić, Milica, Kasas, Sandor, "Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics" in Medicina, 57, no. 5 (2021):446,
https://doi.org/10.3390/medicina57050446 . .

TY  - JOUR
AU  - Venturelli, Leonardo
AU  - Kohler, Anne-Céline
AU  - Stupar, Petar
AU  - Villalba, Maria I.
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
AU  - Bertacchi, Massimiliano
AU  - Dinarelli, Simone
AU  - Girasole, Marco
AU  - Pešić, Milica
AU  - Banković, Jasna
AU  - Vela, Maria E.
AU  - Yantorno, Osvaldo
AU  - Willaert, Ronnie
AU  - Dietler, Giovanni
AU  - Longo, Giovanni
AU  - Kasas, Sandor
PY  - 2020
UR  - http://doi.wiley.com/10.1002/jmr.2849
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3641
AB  - The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die. These nanometric scale oscillations can be detected by depositing living cells onto a micro-fabricated cantilever and by monitoring its displacements with an atomic force microscope-based electronics. Such devices, named nanomotion sensors, have been employed to determine the resistance profiles of life-threatening bacteria within minutes, to evaluate, among others, the effect of chemicals on yeast, neurons, and cancer cells. The data obtained so far demonstrate the advantages of nanomotion sensing devices in rapidly characterizing microorganism susceptibility to pharmaceutical agents. Here, we review the key aspects of this technique, presenting its major applications. and detailing its working protocols.
T2  - Journal of Molecular Recognition
T1  - A perspective view on the nanomotion detection of living organisms and its features
DO  - 10.1002/jmr.2849
SP  - e2849
ER  - 

@article{
author = "Venturelli, Leonardo and Kohler, Anne-Céline and Stupar, Petar and Villalba, Maria I. and Kalauzi, Aleksandar and Radotić, Ksenija and Bertacchi, Massimiliano and Dinarelli, Simone and Girasole, Marco and Pešić, Milica and Banković, Jasna and Vela, Maria E. and Yantorno, Osvaldo and Willaert, Ronnie and Dietler, Giovanni and Longo, Giovanni and Kasas, Sandor",
year = "2020",
abstract = "The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die. These nanometric scale oscillations can be detected by depositing living cells onto a micro-fabricated cantilever and by monitoring its displacements with an atomic force microscope-based electronics. Such devices, named nanomotion sensors, have been employed to determine the resistance profiles of life-threatening bacteria within minutes, to evaluate, among others, the effect of chemicals on yeast, neurons, and cancer cells. The data obtained so far demonstrate the advantages of nanomotion sensing devices in rapidly characterizing microorganism susceptibility to pharmaceutical agents. Here, we review the key aspects of this technique, presenting its major applications. and detailing its working protocols.",
journal = "Journal of Molecular Recognition",
title = "A perspective view on the nanomotion detection of living organisms and its features",
doi = "10.1002/jmr.2849",
pages = "e2849"
}

Venturelli, L., Kohler, A., Stupar, P., Villalba, M. I., Kalauzi, A., Radotić, K., Bertacchi, M., Dinarelli, S., Girasole, M., Pešić, M., Banković, J., Vela, M. E., Yantorno, O., Willaert, R., Dietler, G., Longo, G.,& Kasas, S.. (2020). A perspective view on the nanomotion detection of living organisms and its features. in Journal of Molecular Recognition, e2849.
https://doi.org/10.1002/jmr.2849

Venturelli L, Kohler A, Stupar P, Villalba MI, Kalauzi A, Radotić K, Bertacchi M, Dinarelli S, Girasole M, Pešić M, Banković J, Vela ME, Yantorno O, Willaert R, Dietler G, Longo G, Kasas S. A perspective view on the nanomotion detection of living organisms and its features. in Journal of Molecular Recognition. 2020;:e2849.
doi:10.1002/jmr.2849 .

Venturelli, Leonardo, Kohler, Anne-Céline, Stupar, Petar, Villalba, Maria I., Kalauzi, Aleksandar, Radotić, Ksenija, Bertacchi, Massimiliano, Dinarelli, Simone, Girasole, Marco, Pešić, Milica, Banković, Jasna, Vela, Maria E., Yantorno, Osvaldo, Willaert, Ronnie, Dietler, Giovanni, Longo, Giovanni, Kasas, Sandor, "A perspective view on the nanomotion detection of living organisms and its features" in Journal of Molecular Recognition (2020):e2849,
https://doi.org/10.1002/jmr.2849 . .