TY  - JOUR
AU  - Janković, Aleksandra
AU  - Golic, Igor
AU  - Markelic, Milica
AU  - Stančić, Ana
AU  - Otašević, Vesna
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra
AU  - Korać, Bato
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1921
AB  - Conversion of white into brown adipose tissue may have important
   implications in obesity resistance and treatment. Several browning
   agents or conditions ignite thermogenesis in white adipose tissue (WAT).
   To reveal the capacity of WAT to function in a brownish/burning mode
   over the long term, we investigated the progression of the rat
   retroperitoneal WAT (rpWAT) browning during 45days of cold acclimation.
   During the early stages of cold acclimation, the majority of rpWAT
   adipocytes underwent multilocularization and thermogenic-profile
   induction, as demonstrated by the presence of a multitude of uncoupling
   protein 1 (UCP1)-immunopositive paucilocular adipocytes containing
   peroxisome proliferator-activated receptor (PPAR) coactivator-1 (PGC-1)
   and PR domain-containing 16 (PRDM16) in their nuclei. After 45days, all
   adipocytes remained PRDM16 immunopositive, but only a few multilocular
   adipocytes rich in mitochondria remained UCP1/PGC-1 immunopositive.
   Molecular evidence showed that thermogenic recruitment of rpWAT occurred
   following cold exposure, but returned to starting levels after cold
   acclimation. Compared with controls (22 +/- 1 degrees C), levels of UCP1
   mRNA increased in parallel with PPAR (PPAR from days 1 to 7 and PGC-1 on
   day 1). Transcriptional recruitment of rpWAT was followed by an increase
   in UCP1 protein content (from days 1 to 21). Results clearly showed that
   most of the adipocytes within rpWAT underwent transient brown-fat-like
   thermogenic recruitment upon stimulation, but only a minority of cells
   retained a brown adipose tissue-like phenotype after the attainment of
   cold acclimation. Therefore, browning of WAT is dependent on both
   maintaining the thermogenic response and retaining enough brown-like
   thermogenically competent adipocytes in the long-term. Both aspects of
   browning could be important for long-term energy homeostasis and
   body-weight regulation.
T2  - Journal of Physiology-London
T1  - Two key temporally distinguishable molecular and cellular components of
 white adipose tissue browning during cold acclimation
IS  - 15
VL  - 593
DO  - 10.1113/JP270805
SP  - 3267
EP  - 3280
ER  - 

@article{
author = "Janković, Aleksandra and Golic, Igor and Markelic, Milica and Stančić, Ana and Otašević, Vesna and Buzadžić, Biljana J. and Korac, Aleksandra and Korać, Bato",
year = "2015",
abstract = "Conversion of white into brown adipose tissue may have important
   implications in obesity resistance and treatment. Several browning
   agents or conditions ignite thermogenesis in white adipose tissue (WAT).
   To reveal the capacity of WAT to function in a brownish/burning mode
   over the long term, we investigated the progression of the rat
   retroperitoneal WAT (rpWAT) browning during 45days of cold acclimation.
   During the early stages of cold acclimation, the majority of rpWAT
   adipocytes underwent multilocularization and thermogenic-profile
   induction, as demonstrated by the presence of a multitude of uncoupling
   protein 1 (UCP1)-immunopositive paucilocular adipocytes containing
   peroxisome proliferator-activated receptor (PPAR) coactivator-1 (PGC-1)
   and PR domain-containing 16 (PRDM16) in their nuclei. After 45days, all
   adipocytes remained PRDM16 immunopositive, but only a few multilocular
   adipocytes rich in mitochondria remained UCP1/PGC-1 immunopositive.
   Molecular evidence showed that thermogenic recruitment of rpWAT occurred
   following cold exposure, but returned to starting levels after cold
   acclimation. Compared with controls (22 +/- 1 degrees C), levels of UCP1
   mRNA increased in parallel with PPAR (PPAR from days 1 to 7 and PGC-1 on
   day 1). Transcriptional recruitment of rpWAT was followed by an increase
   in UCP1 protein content (from days 1 to 21). Results clearly showed that
   most of the adipocytes within rpWAT underwent transient brown-fat-like
   thermogenic recruitment upon stimulation, but only a minority of cells
   retained a brown adipose tissue-like phenotype after the attainment of
   cold acclimation. Therefore, browning of WAT is dependent on both
   maintaining the thermogenic response and retaining enough brown-like
   thermogenically competent adipocytes in the long-term. Both aspects of
   browning could be important for long-term energy homeostasis and
   body-weight regulation.",
journal = "Journal of Physiology-London",
title = "Two key temporally distinguishable molecular and cellular components of
 white adipose tissue browning during cold acclimation",
number = "15",
volume = "593",
doi = "10.1113/JP270805",
pages = "3267-3280"
}

Janković, A., Golic, I., Markelic, M., Stančić, A., Otašević, V., Buzadžić, B. J., Korac, A.,& Korać, B.. (2015). Two key temporally distinguishable molecular and cellular components of
 white adipose tissue browning during cold acclimation. in Journal of Physiology-London, 593(15), 3267-3280.
https://doi.org/10.1113/JP270805

Janković A, Golic I, Markelic M, Stančić A, Otašević V, Buzadžić BJ, Korac A, Korać B. Two key temporally distinguishable molecular and cellular components of
 white adipose tissue browning during cold acclimation. in Journal of Physiology-London. 2015;593(15):3267-3280.
doi:10.1113/JP270805 .

Janković, Aleksandra, Golic, Igor, Markelic, Milica, Stančić, Ana, Otašević, Vesna, Buzadžić, Biljana J., Korac, Aleksandra, Korać, Bato, "Two key temporally distinguishable molecular and cellular components of
 white adipose tissue browning during cold acclimation" in Journal of Physiology-London, 593, no. 15 (2015):3267-3280,
https://doi.org/10.1113/JP270805 . .

TY  - JOUR
AU  - Veličković, Ksenija
AU  - Čvoro, Aleksandra
AU  - Srdić, Biljana
AU  - Stokić, Edita
AU  - Markelić, Milica
AU  - Golić, Igor
AU  - Otašević, Vesna
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Buzadžić, Biljana J.
AU  - Korać, Bato
AU  - Korać, Aleksandra
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2294
AB  - Context: Brown adipose tissue (BAT) has the unique ability of generating
   heat due to the expression of mitochondrial uncoupling protein 1 (UCP1).
   A recent discovery regarding functional BAT in adult humans has
   increased interest in the molecular pathways of BAT development and
   functionality. An important role for estrogen in white adipose tissue
   was shown, but the possible role of estrogen in human fetal BAT (fBAT)
   is unclear.
   Objective: The objective of this study was to determine whether human
   fBAT expresses estrogen receptor alpha (ER alpha) and ER beta. In
   addition, we examined their localization as well as their correlation
   with crucial proteins involved in BAT differentiation, proliferation,
   mitochondriogenesis and thermogenesis including peroxisome
   proliferator-activated receptor gamma (PPAR gamma), proliferating cell
   nuclear antigen (PCNA), PPAR gamma-coactivator-1 alpha (PGC-1 alpha),
   and UCP1.
   Design: The fBAT was obtained from 4 human male fetuses aged 15, 17, 20,
   and 23 weeks gestation. ER alpha and ER beta expression was assessed
   using Western blotting, immunohistochemistry, and immunocytochemistry.
   Possible correlations with PPAR gamma, PCNA, PGC-1 alpha, and UCP1 were
   examined by double immunofluorescence.
   Results: Both ER alpha and ER beta were expressed in human fBAT, with ER
   alpha being dominant. Unlike ER beta, which was present only in mature
   brown adipocytes, we detected ER alpha in mature adipocytes,
   preadipocytes, mesenchymal and endothelial cells. In addition, double
   immunofluorescence supported the notion that differentiation in fBAT
   probably involves ER alpha. Immunocytochemical analysis revealed
   mitochondrial localization of both receptors.
   Conclusion: The expression of both ER alpha and ER beta in human fBAT
   suggests a role for estrogen in its development, primarily via ER alpha.
   In addition, our results indicate that fBAT mitochondria could be
   targeted by estrogens and pointed out the possible role of both ERs in
   mitochondriogenesis.
T2  - Journal of Clinical Endocrinology & Metabolism
T1  - Expression and Subcellular Localization of Estrogen Receptors alpha and
 beta in Human Fetal Brown Adipose Tissue
IS  - 1
VL  - 99
DO  - 10.1210/jc.2013-2017
SP  - 151
EP  - 159
ER  - 

@article{
author = "Veličković, Ksenija and Čvoro, Aleksandra and Srdić, Biljana and Stokić, Edita and Markelić, Milica and Golić, Igor and Otašević, Vesna and Stančić, Ana and Janković, Aleksandra and Vučetić, Milica and Buzadžić, Biljana J. and Korać, Bato and Korać, Aleksandra",
year = "2014",
abstract = "Context: Brown adipose tissue (BAT) has the unique ability of generating
   heat due to the expression of mitochondrial uncoupling protein 1 (UCP1).
   A recent discovery regarding functional BAT in adult humans has
   increased interest in the molecular pathways of BAT development and
   functionality. An important role for estrogen in white adipose tissue
   was shown, but the possible role of estrogen in human fetal BAT (fBAT)
   is unclear.
   Objective: The objective of this study was to determine whether human
   fBAT expresses estrogen receptor alpha (ER alpha) and ER beta. In
   addition, we examined their localization as well as their correlation
   with crucial proteins involved in BAT differentiation, proliferation,
   mitochondriogenesis and thermogenesis including peroxisome
   proliferator-activated receptor gamma (PPAR gamma), proliferating cell
   nuclear antigen (PCNA), PPAR gamma-coactivator-1 alpha (PGC-1 alpha),
   and UCP1.
   Design: The fBAT was obtained from 4 human male fetuses aged 15, 17, 20,
   and 23 weeks gestation. ER alpha and ER beta expression was assessed
   using Western blotting, immunohistochemistry, and immunocytochemistry.
   Possible correlations with PPAR gamma, PCNA, PGC-1 alpha, and UCP1 were
   examined by double immunofluorescence.
   Results: Both ER alpha and ER beta were expressed in human fBAT, with ER
   alpha being dominant. Unlike ER beta, which was present only in mature
   brown adipocytes, we detected ER alpha in mature adipocytes,
   preadipocytes, mesenchymal and endothelial cells. In addition, double
   immunofluorescence supported the notion that differentiation in fBAT
   probably involves ER alpha. Immunocytochemical analysis revealed
   mitochondrial localization of both receptors.
   Conclusion: The expression of both ER alpha and ER beta in human fBAT
   suggests a role for estrogen in its development, primarily via ER alpha.
   In addition, our results indicate that fBAT mitochondria could be
   targeted by estrogens and pointed out the possible role of both ERs in
   mitochondriogenesis.",
journal = "Journal of Clinical Endocrinology & Metabolism",
title = "Expression and Subcellular Localization of Estrogen Receptors alpha and
 beta in Human Fetal Brown Adipose Tissue",
number = "1",
volume = "99",
doi = "10.1210/jc.2013-2017",
pages = "151-159"
}

Veličković, K., Čvoro, A., Srdić, B., Stokić, E., Markelić, M., Golić, I., Otašević, V., Stančić, A., Janković, A., Vučetić, M., Buzadžić, B. J., Korać, B.,& Korać, A.. (2014). Expression and Subcellular Localization of Estrogen Receptors alpha and
 beta in Human Fetal Brown Adipose Tissue. in Journal of Clinical Endocrinology & Metabolism, 99(1), 151-159.
https://doi.org/10.1210/jc.2013-2017

Veličković K, Čvoro A, Srdić B, Stokić E, Markelić M, Golić I, Otašević V, Stančić A, Janković A, Vučetić M, Buzadžić BJ, Korać B, Korać A. Expression and Subcellular Localization of Estrogen Receptors alpha and
 beta in Human Fetal Brown Adipose Tissue. in Journal of Clinical Endocrinology & Metabolism. 2014;99(1):151-159.
doi:10.1210/jc.2013-2017 .

Veličković, Ksenija, Čvoro, Aleksandra, Srdić, Biljana, Stokić, Edita, Markelić, Milica, Golić, Igor, Otašević, Vesna, Stančić, Ana, Janković, Aleksandra, Vučetić, Milica, Buzadžić, Biljana J., Korać, Bato, Korać, Aleksandra, "Expression and Subcellular Localization of Estrogen Receptors alpha and
 beta in Human Fetal Brown Adipose Tissue" in Journal of Clinical Endocrinology & Metabolism, 99, no. 1 (2014):151-159,
https://doi.org/10.1210/jc.2013-2017 . .

TY  - JOUR
AU  - Bulatović, Mirna Z.
AU  - Maksimović-Ivanić, Danijela
AU  - Bensing, Christian
AU  - Gomez-Ruiz, Santiago
AU  - Steinborn, Dirk
AU  - Schmidt, Harry
AU  - Mojić, Marija
AU  - Korac, Aleksandra
AU  - Golic, Igor
AU  - Perez-Quintanilla, Damian
AU  - Momčilović, Miljana
AU  - Mijatović, Sanja
AU  - Kaluđerović, Goran N.
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2205
AB  - The strong therapeutic potential of an organotin(IV) compound loaded in
   nanostructured silica (SBA-15pSn) is demonstrated: B16 melanoma tumor
   growth in syngeneic C57BL/6 mice is almost completely abolished. In
   contrast to apoptosis as the basic mechanism of the anticancer action of
   numerous chemotherapeutics, the important advantage of this SBA-15pSn
   mesoporous material is the induction of cell differentiation, an effect
   unknown for metal-based drugs and nanomaterials alone. This
   non-aggressive mode of drug action is highly efficient against cancer
   cells but is in the concentration range used nontoxic for normal tissue.
   JNK (Jun-amino-terminal kinase)-independent apoptosis accompanied by the
   development of the melanocyte-like nonproliferative phenotype of
   survived cells indicates the extraordinary potential of SBA-15pSn to
   suppress tumor growth without undesirable compensatory proliferation of
   malignant cells in response to neighboring cell death.
T2  - Angewandte Chemie-International Edition
T1  - Organotin(IV)-Loaded Mesoporous Silica as a Biocompatible Strategy in
 Cancer Treatment
IS  - 23
VL  - 53
DO  - 10.1002/anie.201400763
SP  - 5982
EP  - 5987
ER  - 

@article{
author = "Bulatović, Mirna Z. and Maksimović-Ivanić, Danijela and Bensing, Christian and Gomez-Ruiz, Santiago and Steinborn, Dirk and Schmidt, Harry and Mojić, Marija and Korac, Aleksandra and Golic, Igor and Perez-Quintanilla, Damian and Momčilović, Miljana and Mijatović, Sanja and Kaluđerović, Goran N.",
year = "2014",
abstract = "The strong therapeutic potential of an organotin(IV) compound loaded in
   nanostructured silica (SBA-15pSn) is demonstrated: B16 melanoma tumor
   growth in syngeneic C57BL/6 mice is almost completely abolished. In
   contrast to apoptosis as the basic mechanism of the anticancer action of
   numerous chemotherapeutics, the important advantage of this SBA-15pSn
   mesoporous material is the induction of cell differentiation, an effect
   unknown for metal-based drugs and nanomaterials alone. This
   non-aggressive mode of drug action is highly efficient against cancer
   cells but is in the concentration range used nontoxic for normal tissue.
   JNK (Jun-amino-terminal kinase)-independent apoptosis accompanied by the
   development of the melanocyte-like nonproliferative phenotype of
   survived cells indicates the extraordinary potential of SBA-15pSn to
   suppress tumor growth without undesirable compensatory proliferation of
   malignant cells in response to neighboring cell death.",
journal = "Angewandte Chemie-International Edition",
title = "Organotin(IV)-Loaded Mesoporous Silica as a Biocompatible Strategy in
 Cancer Treatment",
number = "23",
volume = "53",
doi = "10.1002/anie.201400763",
pages = "5982-5987"
}

Bulatović, M. Z., Maksimović-Ivanić, D., Bensing, C., Gomez-Ruiz, S., Steinborn, D., Schmidt, H., Mojić, M., Korac, A., Golic, I., Perez-Quintanilla, D., Momčilović, M., Mijatović, S.,& Kaluđerović, G. N.. (2014). Organotin(IV)-Loaded Mesoporous Silica as a Biocompatible Strategy in
 Cancer Treatment. in Angewandte Chemie-International Edition, 53(23), 5982-5987.
https://doi.org/10.1002/anie.201400763

Bulatović MZ, Maksimović-Ivanić D, Bensing C, Gomez-Ruiz S, Steinborn D, Schmidt H, Mojić M, Korac A, Golic I, Perez-Quintanilla D, Momčilović M, Mijatović S, Kaluđerović GN. Organotin(IV)-Loaded Mesoporous Silica as a Biocompatible Strategy in
 Cancer Treatment. in Angewandte Chemie-International Edition. 2014;53(23):5982-5987.
doi:10.1002/anie.201400763 .

Bulatović, Mirna Z., Maksimović-Ivanić, Danijela, Bensing, Christian, Gomez-Ruiz, Santiago, Steinborn, Dirk, Schmidt, Harry, Mojić, Marija, Korac, Aleksandra, Golic, Igor, Perez-Quintanilla, Damian, Momčilović, Miljana, Mijatović, Sanja, Kaluđerović, Goran N., "Organotin(IV)-Loaded Mesoporous Silica as a Biocompatible Strategy in
 Cancer Treatment" in Angewandte Chemie-International Edition, 53, no. 23 (2014):5982-5987,
https://doi.org/10.1002/anie.201400763 . .

TY  - JOUR
AU  - Velickovic, Ksenija
AU  - Markelic, Milica
AU  - Golic, Igor
AU  - Otašević, Vesna
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Vucetic, Milica
AU  - Buzadžić, Biljana J.
AU  - Korać, Bato
AU  - Korac, Aleksandra
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2227
AB  - Nitric oxide (NO) and vasoactive intestinal polypeptide (VIP) are
   important intestinal neurotransmitters that coexist in the gut enteric
   nervous system and play an important role in intestinal physiology
   (e.g., absorption, motility, fluid secretion and smooth muscle
   relaxation). It is also known that cold exposure alters several aspects
   of gastrointestinal physiology and induces hyperphagia to meet increased
   metabolic demands, but there are no data regarding NO and VIP
   involvement in intestinal response during acclimation to cold. The
   objective of this study was to determine the influence of long-term
   l-arginine supplementation on the expression of the three isoforms of
   nitric oxide synthase (NOS) and VIP in small intestine of rats
   acclimated to room temperature or cold.
   Animals (six per group) acclimated to room temperature (22 +/- A 1 A
   degrees C) and cold (4 +/- A 1 A degrees C), respectively, were treated
   with 2.25 \% l-arginine, a substrate for NOSs, or with 0.01 \% N
   (omega)-nitro-l-arginine methyl ester, an inhibitor of NOSs, for 45
   days. The topographical distribution of VIP and NOSs expression in small
   intestine was studied by immunohistochemistry, and ImageJ software was
   used for semiquantitative densitometric analysis of their
   immunoexpression.
   Long-term dietary l-arginine supplementation increases VIP and NOSs
   immunoexpression at room temperature while at cold increases the
   endothelial NOS, inducible NOS and VIP but decrease neuronal NOS in rat
   small intestine.
   Our results demonstrate that long-term dietary l-arginine
   supplementation modulates NOSs and VIP immunoexpression in rat small
   intestine with respect to ambient temperature, pointing out the eNOS as
   a predominant NOS isoform with an immunoexpression pattern similar to
   VIP.
T2  - European Journal of Nutrition
T1  - Long-term dietary L-arginine supplementation increases endothelial
 nitric oxide synthase and vasoactive intestinal peptide immunoexpression
 in rat small intestine
IS  - 3
VL  - 53
DO  - 10.1007/s00394-013-0585-8
SP  - 813
EP  - 821
ER  - 

@article{
author = "Velickovic, Ksenija and Markelic, Milica and Golic, Igor and Otašević, Vesna and Stančić, Ana and Janković, Aleksandra and Vucetic, Milica and Buzadžić, Biljana J. and Korać, Bato and Korac, Aleksandra",
year = "2014",
abstract = "Nitric oxide (NO) and vasoactive intestinal polypeptide (VIP) are
   important intestinal neurotransmitters that coexist in the gut enteric
   nervous system and play an important role in intestinal physiology
   (e.g., absorption, motility, fluid secretion and smooth muscle
   relaxation). It is also known that cold exposure alters several aspects
   of gastrointestinal physiology and induces hyperphagia to meet increased
   metabolic demands, but there are no data regarding NO and VIP
   involvement in intestinal response during acclimation to cold. The
   objective of this study was to determine the influence of long-term
   l-arginine supplementation on the expression of the three isoforms of
   nitric oxide synthase (NOS) and VIP in small intestine of rats
   acclimated to room temperature or cold.
   Animals (six per group) acclimated to room temperature (22 +/- A 1 A
   degrees C) and cold (4 +/- A 1 A degrees C), respectively, were treated
   with 2.25 \% l-arginine, a substrate for NOSs, or with 0.01 \% N
   (omega)-nitro-l-arginine methyl ester, an inhibitor of NOSs, for 45
   days. The topographical distribution of VIP and NOSs expression in small
   intestine was studied by immunohistochemistry, and ImageJ software was
   used for semiquantitative densitometric analysis of their
   immunoexpression.
   Long-term dietary l-arginine supplementation increases VIP and NOSs
   immunoexpression at room temperature while at cold increases the
   endothelial NOS, inducible NOS and VIP but decrease neuronal NOS in rat
   small intestine.
   Our results demonstrate that long-term dietary l-arginine
   supplementation modulates NOSs and VIP immunoexpression in rat small
   intestine with respect to ambient temperature, pointing out the eNOS as
   a predominant NOS isoform with an immunoexpression pattern similar to
   VIP.",
journal = "European Journal of Nutrition",
title = "Long-term dietary L-arginine supplementation increases endothelial
 nitric oxide synthase and vasoactive intestinal peptide immunoexpression
 in rat small intestine",
number = "3",
volume = "53",
doi = "10.1007/s00394-013-0585-8",
pages = "813-821"
}

Velickovic, K., Markelic, M., Golic, I., Otašević, V., Stančić, A., Janković, A., Vucetic, M., Buzadžić, B. J., Korać, B.,& Korac, A.. (2014). Long-term dietary L-arginine supplementation increases endothelial
 nitric oxide synthase and vasoactive intestinal peptide immunoexpression
 in rat small intestine. in European Journal of Nutrition, 53(3), 813-821.
https://doi.org/10.1007/s00394-013-0585-8

Velickovic K, Markelic M, Golic I, Otašević V, Stančić A, Janković A, Vucetic M, Buzadžić BJ, Korać B, Korac A. Long-term dietary L-arginine supplementation increases endothelial
 nitric oxide synthase and vasoactive intestinal peptide immunoexpression
 in rat small intestine. in European Journal of Nutrition. 2014;53(3):813-821.
doi:10.1007/s00394-013-0585-8 .

Velickovic, Ksenija, Markelic, Milica, Golic, Igor, Otašević, Vesna, Stančić, Ana, Janković, Aleksandra, Vucetic, Milica, Buzadžić, Biljana J., Korać, Bato, Korac, Aleksandra, "Long-term dietary L-arginine supplementation increases endothelial
 nitric oxide synthase and vasoactive intestinal peptide immunoexpression
 in rat small intestine" in European Journal of Nutrition, 53, no. 3 (2014):813-821,
https://doi.org/10.1007/s00394-013-0585-8 . .

TY  - JOUR
AU  - Janković, Aleksandra
AU  - Korac, Aleksandra
AU  - Srdic-Galic, Biljana
AU  - Buzadžić, Biljana J.
AU  - Otašević, Vesna
AU  - Stančić, Ana
AU  - Vucetic, Milica
AU  - Markelic, Milica
AU  - Velickovic, Ksenija
AU  - Golic, Igor
AU  - Korać, Bato
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2220
AB  - Objective. Metabolic homeostasis depends on adipocyte metabolic
   responses/processes, most of which are redox-regulated. Besides,
   visceral and subcutaneous adipose tissues (VAT and SAT, respectively)
   differ metabolically and in their contribution to metabolic
   complications, but their redox characteristics in humans are still
   unknown. To understand the molecular mechanisms of metabolic syndrome
   development, we analysed the redox characteristics of VAT and SAT in
   groups with various body weights and metabolic risks.
   Material and Methods. Fifty premenopausal women were classified
   according to body mass index into normal-weight and obese groups, and
   these groups were further sub-classified into metabolically healthy and
   metabolically obese ({''}at risk{''}) based on the homeostasis model
   assessment of insulin resistance (HOMA-IR) index and the triglyceride,
   total-, LDL- and HDL-cholesterol levels. Antioxidant components, NADPH
   oxidase protein and 4-hydroxynonenal (4-HNE) levels were analysed in VAT
   and SAT.
   Results. Compared with the SAT, the VAT showed a higher basal level of
   glutathione (GSH) and GSH-dependent enzyme activities. Compared with the
   metabolically healthy normal-weight controls, the obese groups of women
   showed lower GSH levels in both depots. However, in these groups,
   additional prooxidative changes (increased NADPH oxidase and 4-HNE and
   decreased levels of SOD and/or CAT) were observed only in VAT.
   Conclusions. Because of the critical role of thiol-redox homeostasis in
   lipogenesis, interdepot-differences in the GSH-dependent antioxidant
   part may be connected to the higher metabolic activity found in VAT.
   Analogously, the lower GSH levels that occur during obesity and the
   corresponding additional redox imbalance may be signs of VAT metabolic
   dysfunction that underlie the subsequent metabolic impairment. (C) 2014
   Elsevier Inc. All rights reserved.
T2  - Metabolism-Clinical and Experimental
T1  - Differences in the redox status of human visceral and subcutaneous
 adipose tissues - relationships to obesity and metabolic risk
IS  - 5
VL  - 63
DO  - 10.1016/j.metabol.2014.01.009
SP  - 661
EP  - 671
ER  - 

@article{
author = "Janković, Aleksandra and Korac, Aleksandra and Srdic-Galic, Biljana and Buzadžić, Biljana J. and Otašević, Vesna and Stančić, Ana and Vucetic, Milica and Markelic, Milica and Velickovic, Ksenija and Golic, Igor and Korać, Bato",
year = "2014",
abstract = "Objective. Metabolic homeostasis depends on adipocyte metabolic
   responses/processes, most of which are redox-regulated. Besides,
   visceral and subcutaneous adipose tissues (VAT and SAT, respectively)
   differ metabolically and in their contribution to metabolic
   complications, but their redox characteristics in humans are still
   unknown. To understand the molecular mechanisms of metabolic syndrome
   development, we analysed the redox characteristics of VAT and SAT in
   groups with various body weights and metabolic risks.
   Material and Methods. Fifty premenopausal women were classified
   according to body mass index into normal-weight and obese groups, and
   these groups were further sub-classified into metabolically healthy and
   metabolically obese ({''}at risk{''}) based on the homeostasis model
   assessment of insulin resistance (HOMA-IR) index and the triglyceride,
   total-, LDL- and HDL-cholesterol levels. Antioxidant components, NADPH
   oxidase protein and 4-hydroxynonenal (4-HNE) levels were analysed in VAT
   and SAT.
   Results. Compared with the SAT, the VAT showed a higher basal level of
   glutathione (GSH) and GSH-dependent enzyme activities. Compared with the
   metabolically healthy normal-weight controls, the obese groups of women
   showed lower GSH levels in both depots. However, in these groups,
   additional prooxidative changes (increased NADPH oxidase and 4-HNE and
   decreased levels of SOD and/or CAT) were observed only in VAT.
   Conclusions. Because of the critical role of thiol-redox homeostasis in
   lipogenesis, interdepot-differences in the GSH-dependent antioxidant
   part may be connected to the higher metabolic activity found in VAT.
   Analogously, the lower GSH levels that occur during obesity and the
   corresponding additional redox imbalance may be signs of VAT metabolic
   dysfunction that underlie the subsequent metabolic impairment. (C) 2014
   Elsevier Inc. All rights reserved.",
journal = "Metabolism-Clinical and Experimental",
title = "Differences in the redox status of human visceral and subcutaneous
 adipose tissues - relationships to obesity and metabolic risk",
number = "5",
volume = "63",
doi = "10.1016/j.metabol.2014.01.009",
pages = "661-671"
}

Janković, A., Korac, A., Srdic-Galic, B., Buzadžić, B. J., Otašević, V., Stančić, A., Vucetic, M., Markelic, M., Velickovic, K., Golic, I.,& Korać, B.. (2014). Differences in the redox status of human visceral and subcutaneous
 adipose tissues - relationships to obesity and metabolic risk. in Metabolism-Clinical and Experimental, 63(5), 661-671.
https://doi.org/10.1016/j.metabol.2014.01.009

Janković A, Korac A, Srdic-Galic B, Buzadžić BJ, Otašević V, Stančić A, Vucetic M, Markelic M, Velickovic K, Golic I, Korać B. Differences in the redox status of human visceral and subcutaneous
 adipose tissues - relationships to obesity and metabolic risk. in Metabolism-Clinical and Experimental. 2014;63(5):661-671.
doi:10.1016/j.metabol.2014.01.009 .

Janković, Aleksandra, Korac, Aleksandra, Srdic-Galic, Biljana, Buzadžić, Biljana J., Otašević, Vesna, Stančić, Ana, Vucetic, Milica, Markelic, Milica, Velickovic, Ksenija, Golic, Igor, Korać, Bato, "Differences in the redox status of human visceral and subcutaneous
 adipose tissues - relationships to obesity and metabolic risk" in Metabolism-Clinical and Experimental, 63, no. 5 (2014):661-671,
https://doi.org/10.1016/j.metabol.2014.01.009 . .