Popović Minić, Dušanka

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Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization

Popović Minić, Dušanka; Milinčić, Danijel; Kolašinac, Stefan; Rac, Vladislav; Petrović, Jovana; Soković, Marina; Banjac, Nebojša; Lađarević, Jelena; Vidović, Bojana; Kostić, Aleksandar; Pavlović, Vladimir; Pešić, Mirjana

(Oxford: Elsevier, 2023)

TY  - JOUR
AU  - Popović Minić, Dušanka
AU  - Milinčić, Danijel
AU  - Kolašinac, Stefan
AU  - Rac, Vladislav
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Banjac, Nebojša
AU  - Lađarević, Jelena
AU  - Vidović, Bojana
AU  - Kostić, Aleksandar
AU  - Pavlović, Vladimir
AU  - Pešić, Mirjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5140
AB  - This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean particle diameter (from 194 nm to 225 nm). Fourier Transform Infrared Spectroscopy (FTIR) showed that ABM extracts provoked changes in the secondary structure of goat milk proteins and interactions between polysaccharides and milk proteins predominantly via hydrogen and/or glycoside bonds and hydrophobic interactions. The milk protein profiles revealed proteolytic activity in mixtures with AE resulting in the formation of five new polypeptides. The different microstructures of mixtures with AE and PE were found by Scanning Electron Microscopy (SEM). A schematic representation of possible milk proteins-ABM extracts interactions has been given.
PB  - Oxford: Elsevier
T2  - Food Chemistry
T1  - Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization
VL  - 402
DO  - 10.1016/j.foodchem.2022.134299
SP  - 134299
ER  - 
@article{
author = "Popović Minić, Dušanka and Milinčić, Danijel and Kolašinac, Stefan and Rac, Vladislav and Petrović, Jovana and Soković, Marina and Banjac, Nebojša and Lađarević, Jelena and Vidović, Bojana and Kostić, Aleksandar and Pavlović, Vladimir and Pešić, Mirjana",
year = "2023",
abstract = "This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean particle diameter (from 194 nm to 225 nm). Fourier Transform Infrared Spectroscopy (FTIR) showed that ABM extracts provoked changes in the secondary structure of goat milk proteins and interactions between polysaccharides and milk proteins predominantly via hydrogen and/or glycoside bonds and hydrophobic interactions. The milk protein profiles revealed proteolytic activity in mixtures with AE resulting in the formation of five new polypeptides. The different microstructures of mixtures with AE and PE were found by Scanning Electron Microscopy (SEM). A schematic representation of possible milk proteins-ABM extracts interactions has been given.",
publisher = "Oxford: Elsevier",
journal = "Food Chemistry",
title = "Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization",
volume = "402",
doi = "10.1016/j.foodchem.2022.134299",
pages = "134299"
}
Popović Minić, D., Milinčić, D., Kolašinac, S., Rac, V., Petrović, J., Soković, M., Banjac, N., Lađarević, J., Vidović, B., Kostić, A., Pavlović, V.,& Pešić, M.. (2023). Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization. in Food Chemistry
Oxford: Elsevier., 402, 134299.
https://doi.org/10.1016/j.foodchem.2022.134299
Popović Minić D, Milinčić D, Kolašinac S, Rac V, Petrović J, Soković M, Banjac N, Lađarević J, Vidović B, Kostić A, Pavlović V, Pešić M. Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization. in Food Chemistry. 2023;402:134299.
doi:10.1016/j.foodchem.2022.134299 .
Popović Minić, Dušanka, Milinčić, Danijel, Kolašinac, Stefan, Rac, Vladislav, Petrović, Jovana, Soković, Marina, Banjac, Nebojša, Lađarević, Jelena, Vidović, Bojana, Kostić, Aleksandar, Pavlović, Vladimir, Pešić, Mirjana, "Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization" in Food Chemistry, 402 (2023):134299,
https://doi.org/10.1016/j.foodchem.2022.134299 . .
4
4

Zeta-potential and particle size of functional additives based on goat milk proteins and Agaricus blazei Murill extracts.

Popović Minić, Dušanka; Milinčić, Danijel; Rac, Vladislav; Vidović, Bojana; Soković, Marina; Petrović, Jovana; Pešić, Mirjana

(Belgrade: University of Belgrad, 2021)

TY  - CONF
AU  - Popović Minić, Dušanka
AU  - Milinčić, Danijel
AU  - Rac, Vladislav
AU  - Vidović, Bojana
AU  - Soković, Marina
AU  - Petrović, Jovana
AU  - Pešić, Mirjana
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6573
AB  - In recent years, the manufacturing of functional food has gained a lot of attention. The most recent 
studies indicate that bovine milk proteins are suitable carriers for a whole range of bioactive 
substances, mainly because of their micellar structure.
The aim of our study was to examine the possibility of goat milk proteins (RM - raw skimmed milk 
and CN - casein fraction) to deliver A. blazei extracts (VA - hot water extract and GA - glucan 
extract) in order to use all the potential health benefits of this mushroom. Furthermore, the effect of 
thermal treatment (90⁰C, 10 min; TRM – thermally treated RM and TCN - thermally treated CN) on 
carrier properties was also evaluated. The mixture of different goat milk proteins and A. blazei
extracts were prepared and zeta-potential and particle size distribution were determined by a laser 
light-scattering particle size analyzer, using distilled water as a dispersant.
The best results were obtained with the RM and TRM mixtures. It can be concluded that changes in 
particle size and electric charge of the micellar surface occurring in goat milk after heating have a 
positive impact on carrier properties. The particle size of TRM mixtures increased by 13.3 to 15.7% 
compared to the size of TRM particles, whereas the zeta-potential of TRM/VA increased for 6.6% 
compared to that of TRM. TCNs did not follow this trend. The measurement of CNs particle size 
distribution showed the presence of significantly different sizes (coefficient of variation >30%), 
whereas the zeta-potential measurements were stable. These results imply that the measurements of 
zeta-potential cannot be interpreted without the particle size data.
PB  - Belgrade: University of Belgrad
C3  - Book of abstracts: 2nd UNIFood International Conference: UNIFood2021 Conferenece; 2021 Sep 24-25; Belgrade, Serbia
T1  - Zeta-potential and particle size of functional additives based on goat milk proteins and Agaricus blazei Murill extracts.
SP  - 145
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6573
ER  - 
@conference{
author = "Popović Minić, Dušanka and Milinčić, Danijel and Rac, Vladislav and Vidović, Bojana and Soković, Marina and Petrović, Jovana and Pešić, Mirjana",
year = "2021",
abstract = "In recent years, the manufacturing of functional food has gained a lot of attention. The most recent 
studies indicate that bovine milk proteins are suitable carriers for a whole range of bioactive 
substances, mainly because of their micellar structure.
The aim of our study was to examine the possibility of goat milk proteins (RM - raw skimmed milk 
and CN - casein fraction) to deliver A. blazei extracts (VA - hot water extract and GA - glucan 
extract) in order to use all the potential health benefits of this mushroom. Furthermore, the effect of 
thermal treatment (90⁰C, 10 min; TRM – thermally treated RM and TCN - thermally treated CN) on 
carrier properties was also evaluated. The mixture of different goat milk proteins and A. blazei
extracts were prepared and zeta-potential and particle size distribution were determined by a laser 
light-scattering particle size analyzer, using distilled water as a dispersant.
The best results were obtained with the RM and TRM mixtures. It can be concluded that changes in 
particle size and electric charge of the micellar surface occurring in goat milk after heating have a 
positive impact on carrier properties. The particle size of TRM mixtures increased by 13.3 to 15.7% 
compared to the size of TRM particles, whereas the zeta-potential of TRM/VA increased for 6.6% 
compared to that of TRM. TCNs did not follow this trend. The measurement of CNs particle size 
distribution showed the presence of significantly different sizes (coefficient of variation >30%), 
whereas the zeta-potential measurements were stable. These results imply that the measurements of 
zeta-potential cannot be interpreted without the particle size data.",
publisher = "Belgrade: University of Belgrad",
journal = "Book of abstracts: 2nd UNIFood International Conference: UNIFood2021 Conferenece; 2021 Sep 24-25; Belgrade, Serbia",
title = "Zeta-potential and particle size of functional additives based on goat milk proteins and Agaricus blazei Murill extracts.",
pages = "145",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6573"
}
Popović Minić, D., Milinčić, D., Rac, V., Vidović, B., Soković, M., Petrović, J.,& Pešić, M.. (2021). Zeta-potential and particle size of functional additives based on goat milk proteins and Agaricus blazei Murill extracts.. in Book of abstracts: 2nd UNIFood International Conference: UNIFood2021 Conferenece; 2021 Sep 24-25; Belgrade, Serbia
Belgrade: University of Belgrad., 145.
https://hdl.handle.net/21.15107/rcub_ibiss_6573
Popović Minić D, Milinčić D, Rac V, Vidović B, Soković M, Petrović J, Pešić M. Zeta-potential and particle size of functional additives based on goat milk proteins and Agaricus blazei Murill extracts.. in Book of abstracts: 2nd UNIFood International Conference: UNIFood2021 Conferenece; 2021 Sep 24-25; Belgrade, Serbia. 2021;:145.
https://hdl.handle.net/21.15107/rcub_ibiss_6573 .
Popović Minić, Dušanka, Milinčić, Danijel, Rac, Vladislav, Vidović, Bojana, Soković, Marina, Petrović, Jovana, Pešić, Mirjana, "Zeta-potential and particle size of functional additives based on goat milk proteins and Agaricus blazei Murill extracts." in Book of abstracts: 2nd UNIFood International Conference: UNIFood2021 Conferenece; 2021 Sep 24-25; Belgrade, Serbia (2021):145,
https://hdl.handle.net/21.15107/rcub_ibiss_6573 .