TY  - JOUR
AU  - Mićanović, Dragica
AU  - Stanisavljević, Suzana
AU  - Li, Hanluo
AU  - Koprivica, Ivan
AU  - Jonić, Natalija
AU  - Stojanović, Ivana D.
AU  - Savković, Vuk
AU  - Saksida, Tamara
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6829
AB  - Mesenchymal stem cells (MSCs) are known for their immunosuppressive properties.
Based on the demonstrated anti-inflammatory effect of mouse MSCs from hair follicles (moMSCORS) in a murine wound closure model, this study evaluates their potential for preventing type
1 diabetes (T1D) in C57BL/6 mice. T1D was induced in C57BL/6 mice by repeated low doses of streptozotocin. moMSCORS were injected intravenously on weekly basis. moMSCORS reduced T1D incidence, the insulitis stage, and preserved insulin production in treated animals. moMSCORS primarily exerted immunomodulatory effects by inhibiting CD4+ T cell proliferation and activation. Ex
vivo analysis indicated that moMSCORS modified the cellular immune profile within pancreatic
lymph nodes and pancreatic infiltrates by reducing the numbers of M1 pro-inflammatory macrophages and T helper 17 cells and upscaling the immunosuppressive T regulatory cells. The proportion of pathogenic insulin-specific CD4+ T cells was down-scaled in the lymph nodes, likely via soluble factors. The moMSCORS detected in the pancreatic infiltrates of treated mice presumably exerted the observed suppressive effect on CD4+ through direct contact. moMSCORS alleviated T1D
symptoms in the mouse, qualifying as a candidate for therapeutic products by multiple advantages:
non-invasive sampling by epilation, easy access, permanent availability, scalability, and benefits of
auto-transplantation.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Mesenchymal stem cells from mouse hair follicles Inhibit the development of type 1 diabetes
IS  - 11
VL  - 25
DO  - 10.3390/ijms25115974
SP  - 5974
ER  - 

@article{
author = "Mićanović, Dragica and Stanisavljević, Suzana and Li, Hanluo and Koprivica, Ivan and Jonić, Natalija and Stojanović, Ivana D. and Savković, Vuk and Saksida, Tamara",
year = "2024",
abstract = "Mesenchymal stem cells (MSCs) are known for their immunosuppressive properties.
Based on the demonstrated anti-inflammatory effect of mouse MSCs from hair follicles (moMSCORS) in a murine wound closure model, this study evaluates their potential for preventing type
1 diabetes (T1D) in C57BL/6 mice. T1D was induced in C57BL/6 mice by repeated low doses of streptozotocin. moMSCORS were injected intravenously on weekly basis. moMSCORS reduced T1D incidence, the insulitis stage, and preserved insulin production in treated animals. moMSCORS primarily exerted immunomodulatory effects by inhibiting CD4+ T cell proliferation and activation. Ex
vivo analysis indicated that moMSCORS modified the cellular immune profile within pancreatic
lymph nodes and pancreatic infiltrates by reducing the numbers of M1 pro-inflammatory macrophages and T helper 17 cells and upscaling the immunosuppressive T regulatory cells. The proportion of pathogenic insulin-specific CD4+ T cells was down-scaled in the lymph nodes, likely via soluble factors. The moMSCORS detected in the pancreatic infiltrates of treated mice presumably exerted the observed suppressive effect on CD4+ through direct contact. moMSCORS alleviated T1D
symptoms in the mouse, qualifying as a candidate for therapeutic products by multiple advantages:
non-invasive sampling by epilation, easy access, permanent availability, scalability, and benefits of
auto-transplantation.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Mesenchymal stem cells from mouse hair follicles Inhibit the development of type 1 diabetes",
number = "11",
volume = "25",
doi = "10.3390/ijms25115974",
pages = "5974"
}

Mićanović, D., Stanisavljević, S., Li, H., Koprivica, I., Jonić, N., Stojanović, I. D., Savković, V.,& Saksida, T.. (2024). Mesenchymal stem cells from mouse hair follicles Inhibit the development of type 1 diabetes. in International Journal of Molecular Sciences
Basel: MDPI., 25(11), 5974.
https://doi.org/10.3390/ijms25115974

Mićanović D, Stanisavljević S, Li H, Koprivica I, Jonić N, Stojanović ID, Savković V, Saksida T. Mesenchymal stem cells from mouse hair follicles Inhibit the development of type 1 diabetes. in International Journal of Molecular Sciences. 2024;25(11):5974.
doi:10.3390/ijms25115974 .

Mićanović, Dragica, Stanisavljević, Suzana, Li, Hanluo, Koprivica, Ivan, Jonić, Natalija, Stojanović, Ivana D., Savković, Vuk, Saksida, Tamara, "Mesenchymal stem cells from mouse hair follicles Inhibit the development of type 1 diabetes" in International Journal of Molecular Sciences, 25, no. 11 (2024):5974,
https://doi.org/10.3390/ijms25115974 . .

TY  - JOUR
AU  - Pavelegini de Medeiros, Jaqueline
AU  - Alves Rodrigues, Selma
AU  - Sakumoto, Karina
AU  - Ruiz, Suelen Pereira
AU  - Faria, Maria Graciela Iecher
AU  - Gonçalves, José Eduardo
AU  - Junior, Ranulfo Piau
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Dib Gonçalves, Daniela
AU  - Mandim, Filipa
AU  - Barros, Lillian
AU  - Gazim, Zilda Cristiani
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6828
AB  - Introduction: Lung cancer is the most commonly diagnosed and the main cause of cancer death, usually related to cigarette smoking. Furthermore, the microbiota of people exposed to cigarette smoke can be modified, making it difficult to eliminate opportunistic microorganisms. The leaves of Eugenia pyriformis are a by-product of fruit production and, to date, there have been no studies addressing the antiproliferative, anti-inflammatory, and antimicrobial activities.
Objective: Investigate the antimicrobial, Nitric Oxide (NO)-production inhibition, and antiproliferative activities of the essential oil from E. pyriformis leaves and its possible effect on the treatment and prevention of damage caused by tobacco.
Methods: The essential oil (EO) was obtained by hydrodistillation (3 h). Its chemical composition was investigated by GC-MS. It was proposed to investigate antiproliferative activity against human tumor cell lines, namely, breast adenocarcinoma (MCF-7), lung (NCI-H460), cervical (HeLa), and hepatocellular (HepG2) carcinomas. A non-tumor primary culture from pig liver (PLP2) was also tested. The EO capacity to inhibit nitric oxide (NO) production was evaluated by a lipopolysaccharide stimulated murine macrophage cell line. Antibacterial and antifungal activities against opportunistic pathogens were investigated against seven strains of bacteria and eight fungi.
Results: The results indicated the presence of 23 compounds in the essential oil, the majority were spathulenol (45.63%) and β-caryophyllene oxide (12.72%). Leaf EO provided 50% inhibition of nitric oxide production at a concentration of 92.04 µg mL-1. The EO also demonstrated antiproliferative activity against all human tumor cell lines studied, with GI50 values comprised between 270.86 and 337.25 µg mL−1. The essential oil showed antimicrobial potential against the bacteria Listeria monocytogenes (Murray et al.) Pirie (NCTC 7973) and Salmonella Typhimurium ATCC 13311 (MIC 1870 µg mL−1) and fungi Aspergillus versicolor ATCC 11730, Aspergillus ochraceus ATCC 12066, Penicillium ochrochloron ATCC 90288, Penicillium verrucosum var. cyclopium (Westling) Samson, Stolk & Hadlok (food isolate) (MIC 1870 µg mL−1) and Trichoderma viride Pers. IAM 5061 (1,400 µg mL–1).
Conclusion: The demonstrated anti-inflammatory, antiproliferative, and antimicrobial activities in the leaves of E. pyriformis can add value to the production chain of this plant, being a possible option for preventing and combating cancer, including lung cancer.
PB  - Lausanne: Frontiers Media SA
T2  - Frontiers Pharmacology
T1  - Bioactives of the essential oil from the leaves of Eugenia pyriformis Cambess (Myrtaceae) on the effects of tobacco
VL  - 15
DO  - 10.3389/fphar.2024.1415659
SP  - 1415659
ER  - 

@article{
author = "Pavelegini de Medeiros, Jaqueline and Alves Rodrigues, Selma and Sakumoto, Karina and Ruiz, Suelen Pereira and Faria, Maria Graciela Iecher and Gonçalves, José Eduardo and Junior, Ranulfo Piau and Glamočlija, Jasmina and Soković, Marina and Dib Gonçalves, Daniela and Mandim, Filipa and Barros, Lillian and Gazim, Zilda Cristiani",
year = "2024",
abstract = "Introduction: Lung cancer is the most commonly diagnosed and the main cause of cancer death, usually related to cigarette smoking. Furthermore, the microbiota of people exposed to cigarette smoke can be modified, making it difficult to eliminate opportunistic microorganisms. The leaves of Eugenia pyriformis are a by-product of fruit production and, to date, there have been no studies addressing the antiproliferative, anti-inflammatory, and antimicrobial activities.
Objective: Investigate the antimicrobial, Nitric Oxide (NO)-production inhibition, and antiproliferative activities of the essential oil from E. pyriformis leaves and its possible effect on the treatment and prevention of damage caused by tobacco.
Methods: The essential oil (EO) was obtained by hydrodistillation (3 h). Its chemical composition was investigated by GC-MS. It was proposed to investigate antiproliferative activity against human tumor cell lines, namely, breast adenocarcinoma (MCF-7), lung (NCI-H460), cervical (HeLa), and hepatocellular (HepG2) carcinomas. A non-tumor primary culture from pig liver (PLP2) was also tested. The EO capacity to inhibit nitric oxide (NO) production was evaluated by a lipopolysaccharide stimulated murine macrophage cell line. Antibacterial and antifungal activities against opportunistic pathogens were investigated against seven strains of bacteria and eight fungi.
Results: The results indicated the presence of 23 compounds in the essential oil, the majority were spathulenol (45.63%) and β-caryophyllene oxide (12.72%). Leaf EO provided 50% inhibition of nitric oxide production at a concentration of 92.04 µg mL-1. The EO also demonstrated antiproliferative activity against all human tumor cell lines studied, with GI50 values comprised between 270.86 and 337.25 µg mL−1. The essential oil showed antimicrobial potential against the bacteria Listeria monocytogenes (Murray et al.) Pirie (NCTC 7973) and Salmonella Typhimurium ATCC 13311 (MIC 1870 µg mL−1) and fungi Aspergillus versicolor ATCC 11730, Aspergillus ochraceus ATCC 12066, Penicillium ochrochloron ATCC 90288, Penicillium verrucosum var. cyclopium (Westling) Samson, Stolk & Hadlok (food isolate) (MIC 1870 µg mL−1) and Trichoderma viride Pers. IAM 5061 (1,400 µg mL–1).
Conclusion: The demonstrated anti-inflammatory, antiproliferative, and antimicrobial activities in the leaves of E. pyriformis can add value to the production chain of this plant, being a possible option for preventing and combating cancer, including lung cancer.",
publisher = "Lausanne: Frontiers Media SA",
journal = "Frontiers Pharmacology",
title = "Bioactives of the essential oil from the leaves of Eugenia pyriformis Cambess (Myrtaceae) on the effects of tobacco",
volume = "15",
doi = "10.3389/fphar.2024.1415659",
pages = "1415659"
}

Pavelegini de Medeiros, J., Alves Rodrigues, S., Sakumoto, K., Ruiz, S. P., Faria, M. G. I., Gonçalves, J. E., Junior, R. P., Glamočlija, J., Soković, M., Dib Gonçalves, D., Mandim, F., Barros, L.,& Gazim, Z. C.. (2024). Bioactives of the essential oil from the leaves of Eugenia pyriformis Cambess (Myrtaceae) on the effects of tobacco. in Frontiers Pharmacology
Lausanne: Frontiers Media SA., 15, 1415659.
https://doi.org/10.3389/fphar.2024.1415659

Pavelegini de Medeiros J, Alves Rodrigues S, Sakumoto K, Ruiz SP, Faria MGI, Gonçalves JE, Junior RP, Glamočlija J, Soković M, Dib Gonçalves D, Mandim F, Barros L, Gazim ZC. Bioactives of the essential oil from the leaves of Eugenia pyriformis Cambess (Myrtaceae) on the effects of tobacco. in Frontiers Pharmacology. 2024;15:1415659.
doi:10.3389/fphar.2024.1415659 .

Pavelegini de Medeiros, Jaqueline, Alves Rodrigues, Selma, Sakumoto, Karina, Ruiz, Suelen Pereira, Faria, Maria Graciela Iecher, Gonçalves, José Eduardo, Junior, Ranulfo Piau, Glamočlija, Jasmina, Soković, Marina, Dib Gonçalves, Daniela, Mandim, Filipa, Barros, Lillian, Gazim, Zilda Cristiani, "Bioactives of the essential oil from the leaves of Eugenia pyriformis Cambess (Myrtaceae) on the effects of tobacco" in Frontiers Pharmacology, 15 (2024):1415659,
https://doi.org/10.3389/fphar.2024.1415659 . .

TY  - JOUR
AU  - Ninković, Slavica
AU  - Motyka, Václav
AU  - Stanišić, Mariana
AU  - Smailagić, Dijana
AU  - Živanović, Branka
AU  - Dobrev, Petre I.
AU  - Banjac, Nevena
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6826
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6827
AB  - Compounds exuded from roots play a key role in regulating plant allelopathic interactions. However, phytochormone profiling of root exudates and their contribution to an overall allelochemical activity of specific plant species is neglected topic in allelochemical research.  Hairy root growth media of two different species, the fruit tree species  Malus × domestica Borkh. and the herbaceous weed species Chenopodium murale L. were collected and analyzed by high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). We found that most of the phytohormones exuded by the hairy roots of C. murale and M. domestica were associated with the acidic fraction (96.8% and 98.9%, respectively), including 2-oxindole-3-acetic acid, phenylacetic acid, salicylic acid (SA), benzoic acid (BzA), and abscisic acid, with SA and BzA being the most abundant, while those associated with the basic fraction, including cytokinins and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, accounted for only 1% of the plant growth substances detected in both species. Exogenous application of 0.2 µM SA, which was released from the hairy roots of C. murale and accumulated in the culture media for four weeks, significantly impaired hairy root growth of M. domestica and also shoot and root growth of Arabidopsis seedlings. The disruptive effect of 0.2 µM SA on the membrane potential of M. domestica hairy root and Arabidopsis root cells was determined. The data obtained could be useful for planning further studies aimed at clarifying the contribution and role of exuded phytohormones to the overall allelopathic potential of these two plant species.
PB  - New York: Springer
T2  - Journal of Plant Growth Regulation
T1  - Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects
DO  - 10.1007/s00344-024-11328-5
ER  - 

@article{
author = "Ninković, Slavica and Motyka, Václav and Stanišić, Mariana and Smailagić, Dijana and Živanović, Branka and Dobrev, Petre I. and Banjac, Nevena",
year = "2024",
abstract = "Compounds exuded from roots play a key role in regulating plant allelopathic interactions. However, phytochormone profiling of root exudates and their contribution to an overall allelochemical activity of specific plant species is neglected topic in allelochemical research.  Hairy root growth media of two different species, the fruit tree species  Malus × domestica Borkh. and the herbaceous weed species Chenopodium murale L. were collected and analyzed by high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). We found that most of the phytohormones exuded by the hairy roots of C. murale and M. domestica were associated with the acidic fraction (96.8% and 98.9%, respectively), including 2-oxindole-3-acetic acid, phenylacetic acid, salicylic acid (SA), benzoic acid (BzA), and abscisic acid, with SA and BzA being the most abundant, while those associated with the basic fraction, including cytokinins and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, accounted for only 1% of the plant growth substances detected in both species. Exogenous application of 0.2 µM SA, which was released from the hairy roots of C. murale and accumulated in the culture media for four weeks, significantly impaired hairy root growth of M. domestica and also shoot and root growth of Arabidopsis seedlings. The disruptive effect of 0.2 µM SA on the membrane potential of M. domestica hairy root and Arabidopsis root cells was determined. The data obtained could be useful for planning further studies aimed at clarifying the contribution and role of exuded phytohormones to the overall allelopathic potential of these two plant species.",
publisher = "New York: Springer",
journal = "Journal of Plant Growth Regulation",
title = "Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects",
doi = "10.1007/s00344-024-11328-5"
}

Ninković, S., Motyka, V., Stanišić, M., Smailagić, D., Živanović, B., Dobrev, P. I.,& Banjac, N.. (2024). Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects. in Journal of Plant Growth Regulation
New York: Springer..
https://doi.org/10.1007/s00344-024-11328-5

Ninković S, Motyka V, Stanišić M, Smailagić D, Živanović B, Dobrev PI, Banjac N. Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects. in Journal of Plant Growth Regulation. 2024;.
doi:10.1007/s00344-024-11328-5 .

Ninković, Slavica, Motyka, Václav, Stanišić, Mariana, Smailagić, Dijana, Živanović, Branka, Dobrev, Petre I., Banjac, Nevena, "Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects" in Journal of Plant Growth Regulation (2024),
https://doi.org/10.1007/s00344-024-11328-5 . .

TY  - CONF
AU  - Radulović, Natalija
AU  - Kostić, Olga
AU  - Košanin, Olivera
AU  - Pavlović, Dragana
AU  - Sekulić, Dimitrije
AU  - Jonjev, Milica
AU  - Mitrović, Miroslava
AU  - Pavlović, Pavle
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6823
AB  - Linden trees are one of the most popular tree species in European cities and also in Serbia, often used for planting on green areas (parks and tree lines) in Belgrade. In this study, individuals of silver linden Tilia tomentosa Moench. in the tree line (Bulevar Nikola Tesla) and city park (Park Ušće) in Belgrade were selected and the concentration of potentially toxic elements (PTEs) Cu, Pb and Zn in roots and leaves and in the soil (0-10cm and 10-30cm) under each individual as well as in the natural habitat on Fruška Gora mountain (control) were collected. To evaluate the potential of the selected species for phytoremediation (phytoextraction and phytostabilisation of PTEs), the bioaccumulation of roots and leaves (BCFroot and BCFleaf), and translocation factors (TF) were calculated. Site-dependent variations were observed for all analysed parameters. In the soil samples, the concentrations of Cu, Pb and Zn were above the average values for European soils only at urban sites (17.3 mg kg-1, 32 mg kg-1 and 68.1 mg kg-1, respectively) and in the critical range for plants for Zn (>70 mg kg-1) and for Cu (>60 mg kg-1) only in the tree line, while the concentration of Pb was above the MAC. A high correlation was found for the concentration of all tested elements in the soil and in the roots, and only for Zn in the leaves. Nevertheless, the levels of Cu and Pb in the roots and Cu in the leaves were within the normal range for plant tissues (5-30 mg kg-1, 5-10 mg kg-1) at all sites analysed, while Zn was in deficit (<20 mg kg-1). By analysing the bioconcentration and translocation factors for the measured elements, it was found that T. tomentosa has the potential for their phytostabilisation (BCFroot<1, BCFleaf<1, TF>1). These findings could be of great importance in the planning of sustainable management of urban areas.
PB  - Belgrade: Serbian Society of Corrosion and Materials Protection
C3  - Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia
T1  - Evaluation of the phytoremediation potential of Tilia tomentosa Moench. for Cu, Pb and Zn on green areas in Belgrade
SP  - 116
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6823
ER  - 

@conference{
author = "Radulović, Natalija and Kostić, Olga and Košanin, Olivera and Pavlović, Dragana and Sekulić, Dimitrije and Jonjev, Milica and Mitrović, Miroslava and Pavlović, Pavle",
year = "2024",
abstract = "Linden trees are one of the most popular tree species in European cities and also in Serbia, often used for planting on green areas (parks and tree lines) in Belgrade. In this study, individuals of silver linden Tilia tomentosa Moench. in the tree line (Bulevar Nikola Tesla) and city park (Park Ušće) in Belgrade were selected and the concentration of potentially toxic elements (PTEs) Cu, Pb and Zn in roots and leaves and in the soil (0-10cm and 10-30cm) under each individual as well as in the natural habitat on Fruška Gora mountain (control) were collected. To evaluate the potential of the selected species for phytoremediation (phytoextraction and phytostabilisation of PTEs), the bioaccumulation of roots and leaves (BCFroot and BCFleaf), and translocation factors (TF) were calculated. Site-dependent variations were observed for all analysed parameters. In the soil samples, the concentrations of Cu, Pb and Zn were above the average values for European soils only at urban sites (17.3 mg kg-1, 32 mg kg-1 and 68.1 mg kg-1, respectively) and in the critical range for plants for Zn (>70 mg kg-1) and for Cu (>60 mg kg-1) only in the tree line, while the concentration of Pb was above the MAC. A high correlation was found for the concentration of all tested elements in the soil and in the roots, and only for Zn in the leaves. Nevertheless, the levels of Cu and Pb in the roots and Cu in the leaves were within the normal range for plant tissues (5-30 mg kg-1, 5-10 mg kg-1) at all sites analysed, while Zn was in deficit (<20 mg kg-1). By analysing the bioconcentration and translocation factors for the measured elements, it was found that T. tomentosa has the potential for their phytostabilisation (BCFroot<1, BCFleaf<1, TF>1). These findings could be of great importance in the planning of sustainable management of urban areas.",
publisher = "Belgrade: Serbian Society of Corrosion and Materials Protection",
journal = "Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia",
title = "Evaluation of the phytoremediation potential of Tilia tomentosa Moench. for Cu, Pb and Zn on green areas in Belgrade",
pages = "116",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6823"
}

Radulović, N., Kostić, O., Košanin, O., Pavlović, D., Sekulić, D., Jonjev, M., Mitrović, M.,& Pavlović, P.. (2024). Evaluation of the phytoremediation potential of Tilia tomentosa Moench. for Cu, Pb and Zn on green areas in Belgrade. in Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia
Belgrade: Serbian Society of Corrosion and Materials Protection., 116.
https://hdl.handle.net/21.15107/rcub_ibiss_6823

Radulović N, Kostić O, Košanin O, Pavlović D, Sekulić D, Jonjev M, Mitrović M, Pavlović P. Evaluation of the phytoremediation potential of Tilia tomentosa Moench. for Cu, Pb and Zn on green areas in Belgrade. in Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia. 2024;:116.
https://hdl.handle.net/21.15107/rcub_ibiss_6823 .

Radulović, Natalija, Kostić, Olga, Košanin, Olivera, Pavlović, Dragana, Sekulić, Dimitrije, Jonjev, Milica, Mitrović, Miroslava, Pavlović, Pavle, "Evaluation of the phytoremediation potential of Tilia tomentosa Moench. for Cu, Pb and Zn on green areas in Belgrade" in Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia (2024):116,
https://hdl.handle.net/21.15107/rcub_ibiss_6823 .

TY  - CONF
AU  - Matić, Marija
AU  - Pavlović, Dragana
AU  - Perović, Veljko
AU  - Kostić, Olga
AU  - Jarić, Snežana
AU  - Mitrović, Miroslava
AU  - Pavlović, Pavle
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6824
AB  - Vegetable crops are one of the most important foods in the human diet and understanding their ability to accumulate toxic concentrations of hazardous elements can help reduce potential risks to human health. Solanum tuberosum L. is considered the most important non-cereal crop in the world and one of the most important foods in the diet, therefore it is particularly important to identify the potential health risks of consuming vegetables that may be grown in the immediate vicinity of coal mines and thermal power plants. Samples of S. tuberosum and associated soils were taken in the area of the municipalities of Lazarevac (village of Sokolovo) and Obrenovac (village of Krtinka) and Surčin (village of Jakovo) to determine the As and Pb content and to assess the potential human health risk associated with dietary exposure from consumption of the investigated crop. The results obtained showed that the content of non-essential and toxic elements As and Pb was within the range considered normal in plant tissues and below the MAC for dried vegetables under national legislation of the Republic of Serbia. Arsenic was not measured in the tubers of S. tuberosum in 2013, but was measured in the 2014 harvest, while the Pb concentration showed a decreasing trend between the two sampling seasons. The calculation of the non-carcinogenic risk showed that the target hazard quotient and the hazard risk index for S. tuberosum from 2014 exceeded the provisional maximum tolerable daily intake, as the values were >1. These results show that consumption of the 2014 harvest from the investigated sampling sites posed a health risk to humans and urge caution when growing S. tuberosum at the sampling sites.
PB  - Belgrade: Serbian Society of Corrosion and Materials Protection
C3  - Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia
T1  - Assessment of As and Pb in Solanum tuberosum L. from urban areas of Belgrade and potential dietary health risk for the population
SP  - 117
EP  - 123
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6824
ER  - 

@conference{
author = "Matić, Marija and Pavlović, Dragana and Perović, Veljko and Kostić, Olga and Jarić, Snežana and Mitrović, Miroslava and Pavlović, Pavle",
year = "2024",
abstract = "Vegetable crops are one of the most important foods in the human diet and understanding their ability to accumulate toxic concentrations of hazardous elements can help reduce potential risks to human health. Solanum tuberosum L. is considered the most important non-cereal crop in the world and one of the most important foods in the diet, therefore it is particularly important to identify the potential health risks of consuming vegetables that may be grown in the immediate vicinity of coal mines and thermal power plants. Samples of S. tuberosum and associated soils were taken in the area of the municipalities of Lazarevac (village of Sokolovo) and Obrenovac (village of Krtinka) and Surčin (village of Jakovo) to determine the As and Pb content and to assess the potential human health risk associated with dietary exposure from consumption of the investigated crop. The results obtained showed that the content of non-essential and toxic elements As and Pb was within the range considered normal in plant tissues and below the MAC for dried vegetables under national legislation of the Republic of Serbia. Arsenic was not measured in the tubers of S. tuberosum in 2013, but was measured in the 2014 harvest, while the Pb concentration showed a decreasing trend between the two sampling seasons. The calculation of the non-carcinogenic risk showed that the target hazard quotient and the hazard risk index for S. tuberosum from 2014 exceeded the provisional maximum tolerable daily intake, as the values were >1. These results show that consumption of the 2014 harvest from the investigated sampling sites posed a health risk to humans and urge caution when growing S. tuberosum at the sampling sites.",
publisher = "Belgrade: Serbian Society of Corrosion and Materials Protection",
journal = "Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia",
title = "Assessment of As and Pb in Solanum tuberosum L. from urban areas of Belgrade and potential dietary health risk for the population",
pages = "117-123",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6824"
}

Matić, M., Pavlović, D., Perović, V., Kostić, O., Jarić, S., Mitrović, M.,& Pavlović, P.. (2024). Assessment of As and Pb in Solanum tuberosum L. from urban areas of Belgrade and potential dietary health risk for the population. in Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia
Belgrade: Serbian Society of Corrosion and Materials Protection., 117-123.
https://hdl.handle.net/21.15107/rcub_ibiss_6824

Matić M, Pavlović D, Perović V, Kostić O, Jarić S, Mitrović M, Pavlović P. Assessment of As and Pb in Solanum tuberosum L. from urban areas of Belgrade and potential dietary health risk for the population. in Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia. 2024;:117-123.
https://hdl.handle.net/21.15107/rcub_ibiss_6824 .

Matić, Marija, Pavlović, Dragana, Perović, Veljko, Kostić, Olga, Jarić, Snežana, Mitrović, Miroslava, Pavlović, Pavle, "Assessment of As and Pb in Solanum tuberosum L. from urban areas of Belgrade and potential dietary health risk for the population" in Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia (2024):117-123,
https://hdl.handle.net/21.15107/rcub_ibiss_6824 .

TY  - CONF
AU  - Pavlović, Dragana
AU  - Matić, Marija
AU  - Perović, Veljko
AU  - Jarić, Snežana
AU  - Kostić, Olga
AU  - Mitrović, Miroslava
AU  - Pavlović, Pavle
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6825
AB  - The health consequences of eating contaminated vegetables for the population are a major problem worldwide. In this study, As and Pb concentrations in soil and in Allium cepa L. (spring onions and onions) from three Belgrade municipalities were determined using an inductively coupled plasma optical emission spectrophotometer (ICP-OES) and possible health risks from onion consumption were assessed. The results showed that As and Pb concentrations in the soil were within the maximum levels proposed by national legislation; the highest concentrations were measured in the soil of Lazarevac. It was found that although A. cepa has a low potential to accumulate As and Pb, this vegetable accumulates As and Pb at levels well above the limits set by the World Health Organisation, so great caution should be exercised when consuming it. The consumption of spring onions could lead to serious health problems over time, especially with regard to the increased As content, although the onions have been shown to be safe for consumption. This study could contribute to a better understanding of the health risks associated with the consumption of vegetables grown near urban and industrial areas.
PB  - Belgrade: Serbian Society of Corrosion and Materials Protection
C3  - Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia
T1  - Levels of As and Pb in soil and Allium cepa L. and associated health risk in three Belgrade municipalities
SP  - 39
EP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6825
ER  - 

@conference{
author = "Pavlović, Dragana and Matić, Marija and Perović, Veljko and Jarić, Snežana and Kostić, Olga and Mitrović, Miroslava and Pavlović, Pavle",
year = "2024",
abstract = "The health consequences of eating contaminated vegetables for the population are a major problem worldwide. In this study, As and Pb concentrations in soil and in Allium cepa L. (spring onions and onions) from three Belgrade municipalities were determined using an inductively coupled plasma optical emission spectrophotometer (ICP-OES) and possible health risks from onion consumption were assessed. The results showed that As and Pb concentrations in the soil were within the maximum levels proposed by national legislation; the highest concentrations were measured in the soil of Lazarevac. It was found that although A. cepa has a low potential to accumulate As and Pb, this vegetable accumulates As and Pb at levels well above the limits set by the World Health Organisation, so great caution should be exercised when consuming it. The consumption of spring onions could lead to serious health problems over time, especially with regard to the increased As content, although the onions have been shown to be safe for consumption. This study could contribute to a better understanding of the health risks associated with the consumption of vegetables grown near urban and industrial areas.",
publisher = "Belgrade: Serbian Society of Corrosion and Materials Protection",
journal = "Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia",
title = "Levels of As and Pb in soil and Allium cepa L. and associated health risk in three Belgrade municipalities",
pages = "39-44",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6825"
}

Pavlović, D., Matić, M., Perović, V., Jarić, S., Kostić, O., Mitrović, M.,& Pavlović, P.. (2024). Levels of As and Pb in soil and Allium cepa L. and associated health risk in three Belgrade municipalities. in Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia
Belgrade: Serbian Society of Corrosion and Materials Protection., 39-44.
https://hdl.handle.net/21.15107/rcub_ibiss_6825

Pavlović D, Matić M, Perović V, Jarić S, Kostić O, Mitrović M, Pavlović P. Levels of As and Pb in soil and Allium cepa L. and associated health risk in three Belgrade municipalities. in Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia. 2024;:39-44.
https://hdl.handle.net/21.15107/rcub_ibiss_6825 .

Pavlović, Dragana, Matić, Marija, Perović, Veljko, Jarić, Snežana, Kostić, Olga, Mitrović, Miroslava, Pavlović, Pavle, "Levels of As and Pb in soil and Allium cepa L. and associated health risk in three Belgrade municipalities" in Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia (2024):39-44,
https://hdl.handle.net/21.15107/rcub_ibiss_6825 .

TY  - JOUR
AU  - Ninković, Slavica
AU  - Motyka, Václav
AU  - Stanišić, Mariana
AU  - Smailagić, Dijana
AU  - Živanović, Branka
AU  - Dobrev, Petre I.
AU  - Banjac, Nevena
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6826
AB  - Compounds exuded from roots play a key role in regulating plant allelopathic interactions. However, phytochormone profiling of root exudates and their contribution to an overall allelochemical activity of specific plant species is neglected topic in allelochemical research.  Hairy root growth media of two different species, the fruit tree species  Malus × domestica Borkh. and the herbaceous weed species Chenopodium murale L. were collected and analyzed by high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). We found that most of the phytohormones exuded by the hairy roots of C. murale and M. domestica were associated with the acidic fraction (96.8% and 98.9%, respectively), including 2-oxindole-3-acetic acid, phenylacetic acid, salicylic acid (SA), benzoic acid (BzA), and abscisic acid, with SA and BzA being the most abundant, while those associated with the basic fraction, including cytokinins and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, accounted for only 1% of the plant growth substances detected in both species. Exogenous application of 0.2 µM SA, which was released from the hairy roots of C. murale and accumulated in the culture media for four weeks, significantly impaired hairy root growth of M. domestica and also shoot and root growth of Arabidopsis seedlings. The disruptive effect of 0.2 µM SA on the membrane potential of M. domestica hairy root and Arabidopsis root cells was determined. The data obtained could be useful for planning further studies aimed at clarifying the contribution and role of exuded phytohormones to the overall allelopathic potential of these two plant species.
PB  - New York: Springer
T2  - Journal of Plant Growth Regulation
T1  - Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects
DO  - 10.1007/s00344-024-11328-5
ER  - 

@article{
author = "Ninković, Slavica and Motyka, Václav and Stanišić, Mariana and Smailagić, Dijana and Živanović, Branka and Dobrev, Petre I. and Banjac, Nevena",
year = "2024",
abstract = "Compounds exuded from roots play a key role in regulating plant allelopathic interactions. However, phytochormone profiling of root exudates and their contribution to an overall allelochemical activity of specific plant species is neglected topic in allelochemical research.  Hairy root growth media of two different species, the fruit tree species  Malus × domestica Borkh. and the herbaceous weed species Chenopodium murale L. were collected and analyzed by high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). We found that most of the phytohormones exuded by the hairy roots of C. murale and M. domestica were associated with the acidic fraction (96.8% and 98.9%, respectively), including 2-oxindole-3-acetic acid, phenylacetic acid, salicylic acid (SA), benzoic acid (BzA), and abscisic acid, with SA and BzA being the most abundant, while those associated with the basic fraction, including cytokinins and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, accounted for only 1% of the plant growth substances detected in both species. Exogenous application of 0.2 µM SA, which was released from the hairy roots of C. murale and accumulated in the culture media for four weeks, significantly impaired hairy root growth of M. domestica and also shoot and root growth of Arabidopsis seedlings. The disruptive effect of 0.2 µM SA on the membrane potential of M. domestica hairy root and Arabidopsis root cells was determined. The data obtained could be useful for planning further studies aimed at clarifying the contribution and role of exuded phytohormones to the overall allelopathic potential of these two plant species.",
publisher = "New York: Springer",
journal = "Journal of Plant Growth Regulation",
title = "Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects",
doi = "10.1007/s00344-024-11328-5"
}

Ninković, S., Motyka, V., Stanišić, M., Smailagić, D., Živanović, B., Dobrev, P. I.,& Banjac, N.. (2024). Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects. in Journal of Plant Growth Regulation
New York: Springer..
https://doi.org/10.1007/s00344-024-11328-5

Ninković S, Motyka V, Stanišić M, Smailagić D, Živanović B, Dobrev PI, Banjac N. Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects. in Journal of Plant Growth Regulation. 2024;.
doi:10.1007/s00344-024-11328-5 .

Ninković, Slavica, Motyka, Václav, Stanišić, Mariana, Smailagić, Dijana, Živanović, Branka, Dobrev, Petre I., Banjac, Nevena, "Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects" in Journal of Plant Growth Regulation (2024),
https://doi.org/10.1007/s00344-024-11328-5 . .

TY  - JOUR
AU  - Baur, Julian
AU  - Koppik, Mareike
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Stojković, Biljana
AU  - Berger, David
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6820
AB  - An often-overlooked aspect of life-history optimization is the allocation of resources to protect the germline and secure safe transmission of genetic information. While failure to do so renders significant fitness consequences in future generations, germline maintenance comes with substantial costs. Thus, germline allocation should trade off with other life-history decisions and be optimized in accordance with an organism’s reproductive schedule. Here, we tested this hypothesis by studying germline maintenance in lines of seed beetle, selected for early (E) or late (L) reproduction for 350 and 240 generations, respectively. Female animals provide maintenance and screening of male gametes in their reproductive tract and oocytes. Here, we reveal the ability of young and aged E- and L-females to provide this form of germline maintenance by mating them to males with ejaculates with artificially elevated levels of protein and DNA damage. We find that germline maintenance in E-females peaks at young age and then declines, while the opposite is true for L-females, in accordance with the age of reproduction in the respective regime. These findings identify the central role of allocation to secure germline integrity in life-history evolution and highlight how females can play a crucial role in mitigating the effects of male germline decisions on mutation rate and offspring quality.
PB  - The Royal Society
T2  - Proceedings of the Royal Society. B: Biological Sciences
T1  - Coevolution of longevity and femalegermline maintenance
IS  - 2024
VL  - 291
DO  - 10.1098/rspb.2024.0532
SP  - 20240532
ER  - 

@article{
author = "Baur, Julian and Koppik, Mareike and Savković, Uroš and Đorđević, Mirko and Stojković, Biljana and Berger, David",
year = "2024",
abstract = "An often-overlooked aspect of life-history optimization is the allocation of resources to protect the germline and secure safe transmission of genetic information. While failure to do so renders significant fitness consequences in future generations, germline maintenance comes with substantial costs. Thus, germline allocation should trade off with other life-history decisions and be optimized in accordance with an organism’s reproductive schedule. Here, we tested this hypothesis by studying germline maintenance in lines of seed beetle, selected for early (E) or late (L) reproduction for 350 and 240 generations, respectively. Female animals provide maintenance and screening of male gametes in their reproductive tract and oocytes. Here, we reveal the ability of young and aged E- and L-females to provide this form of germline maintenance by mating them to males with ejaculates with artificially elevated levels of protein and DNA damage. We find that germline maintenance in E-females peaks at young age and then declines, while the opposite is true for L-females, in accordance with the age of reproduction in the respective regime. These findings identify the central role of allocation to secure germline integrity in life-history evolution and highlight how females can play a crucial role in mitigating the effects of male germline decisions on mutation rate and offspring quality.",
publisher = "The Royal Society",
journal = "Proceedings of the Royal Society. B: Biological Sciences",
title = "Coevolution of longevity and femalegermline maintenance",
number = "2024",
volume = "291",
doi = "10.1098/rspb.2024.0532",
pages = "20240532"
}

Baur, J., Koppik, M., Savković, U., Đorđević, M., Stojković, B.,& Berger, D.. (2024). Coevolution of longevity and femalegermline maintenance. in Proceedings of the Royal Society. B: Biological Sciences
The Royal Society., 291(2024), 20240532.
https://doi.org/10.1098/rspb.2024.0532

Baur J, Koppik M, Savković U, Đorđević M, Stojković B, Berger D. Coevolution of longevity and femalegermline maintenance. in Proceedings of the Royal Society. B: Biological Sciences. 2024;291(2024):20240532.
doi:10.1098/rspb.2024.0532 .

Baur, Julian, Koppik, Mareike, Savković, Uroš, Đorđević, Mirko, Stojković, Biljana, Berger, David, "Coevolution of longevity and femalegermline maintenance" in Proceedings of the Royal Society. B: Biological Sciences, 291, no. 2024 (2024):20240532,
https://doi.org/10.1098/rspb.2024.0532 . .

TY  - JOUR
AU  - Kasalović, Marijana P.
AU  - Jelača, Sanja
AU  - Milanović, Žiko
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Lađarević, Jelena
AU  - Božić, Bojan
AU  - Marković, Zoran
AU  - Dunđerović, Duško
AU  - Rüffer, Tobias
AU  - Kretschmer, Robert
AU  - Kaluđerović, Goran N.
AU  - Pantelić, Nebojša Đ.
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6821
AB  - Three newly synthesized triphenyltin(IV) compounds, Ph3SnL1 (L1− = 3-(4-methyl-2-oxoquinolin-1(2H)-yl)propanoato), Ph3SnL2 (L2− = 2-(4-methyl-2-oxoquinolin-1(2H)-yl)ethanoato), and Ph3SnL3 (L3− = 2-(4-hydroxy-2-oxoquinolin-1(2H)-yl)ethanoato), were characterized by elemental microanalysis, FT-IR spectroscopy and multinuclear (1H, 13C and 119Sn) NMR spectroscopy. A single X-ray diffraction study indicates that compounds Ph3SnL1 and Ph3SnL2 exhibit a 1D zig-zag chain polymeric structure, which in the case of Ph3SnL2 is additionally stabilized by π-interactions. In addition, the synthesized compounds were further examined using density functional theory and natural bond orbital analysis. The compounds have been evaluated for their in vitro anticancer activity against three human cell lines: MCF-7 (breast adenocarcinoma), A375 (melanoma), HCT116 (colorectal carcinoma), and three murine cell lines: 4T1 (breast carcinoma), B16 (melanoma), CT26 (colon carcinoma) using MTT and CV assays. The IC50 values fall in the nanomolar range, indicating that these compounds possess better anticancer activity than cisplatin. The study of the effect of the newly developed drug Ph3SnL1 showed its plasticity in achieving an antitumor effect in vitro, which depends on the specificity of the phenotype and the redox status of the malignant cell line and ranges from the initiation of apoptotic cell death to the induction of differentiation to a more mature cell form. In the syngeneic model of murine melanoma, Ph3SnL1 showed the potential to reduce the tumor volume similar to cisplatin, but in a well-tolerated form and with low systemic toxicity, representing a significant advantage over the conventional drug.
PB  - Cambridge: The Royal Society of Chemistry
T2  - Dalton Transactions
T1  - Novel triphenyltin(iv) compounds with carboxylato N-functionalized 2-quinolones as promising potential anticancer drug candidates: in vitro and in vivo evaluation
IS  - 19
VL  - 53
DO  - 10.1039/D4DT00182F
SP  - 8298
EP  - 8314
ER  - 

@article{
author = "Kasalović, Marijana P. and Jelača, Sanja and Milanović, Žiko and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Lađarević, Jelena and Božić, Bojan and Marković, Zoran and Dunđerović, Duško and Rüffer, Tobias and Kretschmer, Robert and Kaluđerović, Goran N. and Pantelić, Nebojša Đ.",
year = "2024",
abstract = "Three newly synthesized triphenyltin(IV) compounds, Ph3SnL1 (L1− = 3-(4-methyl-2-oxoquinolin-1(2H)-yl)propanoato), Ph3SnL2 (L2− = 2-(4-methyl-2-oxoquinolin-1(2H)-yl)ethanoato), and Ph3SnL3 (L3− = 2-(4-hydroxy-2-oxoquinolin-1(2H)-yl)ethanoato), were characterized by elemental microanalysis, FT-IR spectroscopy and multinuclear (1H, 13C and 119Sn) NMR spectroscopy. A single X-ray diffraction study indicates that compounds Ph3SnL1 and Ph3SnL2 exhibit a 1D zig-zag chain polymeric structure, which in the case of Ph3SnL2 is additionally stabilized by π-interactions. In addition, the synthesized compounds were further examined using density functional theory and natural bond orbital analysis. The compounds have been evaluated for their in vitro anticancer activity against three human cell lines: MCF-7 (breast adenocarcinoma), A375 (melanoma), HCT116 (colorectal carcinoma), and three murine cell lines: 4T1 (breast carcinoma), B16 (melanoma), CT26 (colon carcinoma) using MTT and CV assays. The IC50 values fall in the nanomolar range, indicating that these compounds possess better anticancer activity than cisplatin. The study of the effect of the newly developed drug Ph3SnL1 showed its plasticity in achieving an antitumor effect in vitro, which depends on the specificity of the phenotype and the redox status of the malignant cell line and ranges from the initiation of apoptotic cell death to the induction of differentiation to a more mature cell form. In the syngeneic model of murine melanoma, Ph3SnL1 showed the potential to reduce the tumor volume similar to cisplatin, but in a well-tolerated form and with low systemic toxicity, representing a significant advantage over the conventional drug.",
publisher = "Cambridge: The Royal Society of Chemistry",
journal = "Dalton Transactions",
title = "Novel triphenyltin(iv) compounds with carboxylato N-functionalized 2-quinolones as promising potential anticancer drug candidates: in vitro and in vivo evaluation",
number = "19",
volume = "53",
doi = "10.1039/D4DT00182F",
pages = "8298-8314"
}

Kasalović, M. P., Jelača, S., Milanović, Ž., Maksimović-Ivanić, D., Mijatović, S., Lađarević, J., Božić, B., Marković, Z., Dunđerović, D., Rüffer, T., Kretschmer, R., Kaluđerović, G. N.,& Pantelić, N. Đ.. (2024). Novel triphenyltin(iv) compounds with carboxylato N-functionalized 2-quinolones as promising potential anticancer drug candidates: in vitro and in vivo evaluation. in Dalton Transactions
Cambridge: The Royal Society of Chemistry., 53(19), 8298-8314.
https://doi.org/10.1039/D4DT00182F

Kasalović MP, Jelača S, Milanović Ž, Maksimović-Ivanić D, Mijatović S, Lađarević J, Božić B, Marković Z, Dunđerović D, Rüffer T, Kretschmer R, Kaluđerović GN, Pantelić NĐ. Novel triphenyltin(iv) compounds with carboxylato N-functionalized 2-quinolones as promising potential anticancer drug candidates: in vitro and in vivo evaluation. in Dalton Transactions. 2024;53(19):8298-8314.
doi:10.1039/D4DT00182F .

Kasalović, Marijana P., Jelača, Sanja, Milanović, Žiko, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Lađarević, Jelena, Božić, Bojan, Marković, Zoran, Dunđerović, Duško, Rüffer, Tobias, Kretschmer, Robert, Kaluđerović, Goran N., Pantelić, Nebojša Đ., "Novel triphenyltin(iv) compounds with carboxylato N-functionalized 2-quinolones as promising potential anticancer drug candidates: in vitro and in vivo evaluation" in Dalton Transactions, 53, no. 19 (2024):8298-8314,
https://doi.org/10.1039/D4DT00182F . .

TY  - JOUR
AU  - Stanisavljević, Suzana
AU  - Stegnjaić, Goran
AU  - Jevtić, Bojan
AU  - Dimitrijević, Mirjana
AU  - Miljković, Đorđe
AU  - Lavrnja, Irena
AU  - Nikolovski, Neda
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6808
AB  - Ethyl pyruvate (EP) is a redox-active compound that has been previously shown to be
effective in restraining immune hyperactivity in animal models of various autoimmune and chronic
inflammatory diseases. Importantly, EP has also been proven to have a potent tolerogenic effect
on dendritic cells (DCs). Here, the influence of EP on the signaling pathways in DCs relevant for
their tolerogenicity, including anti-inflammatory NRF2 and pro-inflammatory NF-κB, was explored.
Specifically, the effects of EP on DCs obtained by GM-CSF-directed differentiation of murine bone
marrow precursor cells and matured under the influence of lipopolysaccharide (LPS) were examined via immunocytochemistry and RT-PCR. EP counteracted LPS-imposed morphological changes
and down-regulated the LPS-induced expression of pro-inflammatory mediators in DCs. While it
reduced the activation of NF-κB, EP potentiated NRF2 and downstream antioxidative molecules,
thus implying the regulation of NRF2 signaling pathways as the major reason for the tolerizing
effects of EP on DCs.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - NRF2 plays a crucial role in the tolerogenic effect of ethyl pyruvate on dendritic cells
IS  - 11
VL  - 25
DO  - 10.3390/ijms25116195
SP  - 6195
ER  - 

@article{
author = "Stanisavljević, Suzana and Stegnjaić, Goran and Jevtić, Bojan and Dimitrijević, Mirjana and Miljković, Đorđe and Lavrnja, Irena and Nikolovski, Neda",
year = "2024",
abstract = "Ethyl pyruvate (EP) is a redox-active compound that has been previously shown to be
effective in restraining immune hyperactivity in animal models of various autoimmune and chronic
inflammatory diseases. Importantly, EP has also been proven to have a potent tolerogenic effect
on dendritic cells (DCs). Here, the influence of EP on the signaling pathways in DCs relevant for
their tolerogenicity, including anti-inflammatory NRF2 and pro-inflammatory NF-κB, was explored.
Specifically, the effects of EP on DCs obtained by GM-CSF-directed differentiation of murine bone
marrow precursor cells and matured under the influence of lipopolysaccharide (LPS) were examined via immunocytochemistry and RT-PCR. EP counteracted LPS-imposed morphological changes
and down-regulated the LPS-induced expression of pro-inflammatory mediators in DCs. While it
reduced the activation of NF-κB, EP potentiated NRF2 and downstream antioxidative molecules,
thus implying the regulation of NRF2 signaling pathways as the major reason for the tolerizing
effects of EP on DCs.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "NRF2 plays a crucial role in the tolerogenic effect of ethyl pyruvate on dendritic cells",
number = "11",
volume = "25",
doi = "10.3390/ijms25116195",
pages = "6195"
}

Stanisavljević, S., Stegnjaić, G., Jevtić, B., Dimitrijević, M., Miljković, Đ., Lavrnja, I.,& Nikolovski, N.. (2024). NRF2 plays a crucial role in the tolerogenic effect of ethyl pyruvate on dendritic cells. in International Journal of Molecular Sciences
Basel: MDPI., 25(11), 6195.
https://doi.org/10.3390/ijms25116195

Stanisavljević S, Stegnjaić G, Jevtić B, Dimitrijević M, Miljković Đ, Lavrnja I, Nikolovski N. NRF2 plays a crucial role in the tolerogenic effect of ethyl pyruvate on dendritic cells. in International Journal of Molecular Sciences. 2024;25(11):6195.
doi:10.3390/ijms25116195 .

Stanisavljević, Suzana, Stegnjaić, Goran, Jevtić, Bojan, Dimitrijević, Mirjana, Miljković, Đorđe, Lavrnja, Irena, Nikolovski, Neda, "NRF2 plays a crucial role in the tolerogenic effect of ethyl pyruvate on dendritic cells" in International Journal of Molecular Sciences, 25, no. 11 (2024):6195,
https://doi.org/10.3390/ijms25116195 . .

TY  - JOUR
AU  - Popržen, Tatjana
AU  - Jevremović, Slađana
AU  - Milošević, Snežana
AU  - Đurić, Marija
AU  - Uzelac, Branka
AU  - Stanković, Slaviša
AU  - Radulović, Olga
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6796
AB  - Aquatic plants, just like terrestrial plants, are exposed to oxidative stress. However,
their responses are still under-researched. In this study, we examined the physiological and antioxidative
responses of an aquatic plant, duckweed (Lemna minor L.), to four indole-3-acetic acid
(IAA)-degrading and -producing Pseudomonas bacteria (Pseudomonas oryzihabitans D1-104/3, P. putida
A3-104/5, P. gessardii C31-106/3 and P. yamanorum C44-104/1) and/or a supraphysiological level
of IAA (10 mg L−1). Growth characteristics, total photosynthetic pigment content, histochemical
localization of reactive oxygen species and antioxidant enzyme activity (SOD, CAT and POX) were
evaluated at two time points, after 3 and 7 days of co-cultivation. Superoxide anion and hydrogen
peroxide were produced and accumulated mainly in the roots, daughter fronds and veins of duckweeds.
Duckweeds’ responses depended on the strain of Pseudomonas, time and exogenous IAA.
Co-cultivation of duckweed with bacteria has positive or neutral effects. Exogenous application
of IAA had a negative or neutral effect on enzyme activity and other parameters. Co-cultivation
with P. gessardii C31-106/3 showed plant-growth-promoting effects on duckweed: increased biomass
production, modulation of duckweeds’ antioxidant enzymatic activity and reduction in hydrogen
peroxide content. This study widens our knowledge of aquatic plants and their response to oxidative
stress, supports the hypothesis that plant growth-promoting bacteria (PGPB) induce tolerable levels
of oxidative stress in plants and introduces a new PGPB strain, P. gessardii C31-106/3.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Antioxidative Response of Duckweed (Lemna minor L.) to Rhizosphere-Associated Pseudomonas Strains and Exogenous Indole-3-Acetic Acid
IS  - 6
VL  - 10
DO  - 10.3390/horticulturae10060562
SP  - 562
ER  - 

@article{
author = "Popržen, Tatjana and Jevremović, Slađana and Milošević, Snežana and Đurić, Marija and Uzelac, Branka and Stanković, Slaviša and Radulović, Olga",
year = "2024",
abstract = "Aquatic plants, just like terrestrial plants, are exposed to oxidative stress. However,
their responses are still under-researched. In this study, we examined the physiological and antioxidative
responses of an aquatic plant, duckweed (Lemna minor L.), to four indole-3-acetic acid
(IAA)-degrading and -producing Pseudomonas bacteria (Pseudomonas oryzihabitans D1-104/3, P. putida
A3-104/5, P. gessardii C31-106/3 and P. yamanorum C44-104/1) and/or a supraphysiological level
of IAA (10 mg L−1). Growth characteristics, total photosynthetic pigment content, histochemical
localization of reactive oxygen species and antioxidant enzyme activity (SOD, CAT and POX) were
evaluated at two time points, after 3 and 7 days of co-cultivation. Superoxide anion and hydrogen
peroxide were produced and accumulated mainly in the roots, daughter fronds and veins of duckweeds.
Duckweeds’ responses depended on the strain of Pseudomonas, time and exogenous IAA.
Co-cultivation of duckweed with bacteria has positive or neutral effects. Exogenous application
of IAA had a negative or neutral effect on enzyme activity and other parameters. Co-cultivation
with P. gessardii C31-106/3 showed plant-growth-promoting effects on duckweed: increased biomass
production, modulation of duckweeds’ antioxidant enzymatic activity and reduction in hydrogen
peroxide content. This study widens our knowledge of aquatic plants and their response to oxidative
stress, supports the hypothesis that plant growth-promoting bacteria (PGPB) induce tolerable levels
of oxidative stress in plants and introduces a new PGPB strain, P. gessardii C31-106/3.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Antioxidative Response of Duckweed (Lemna minor L.) to Rhizosphere-Associated Pseudomonas Strains and Exogenous Indole-3-Acetic Acid",
number = "6",
volume = "10",
doi = "10.3390/horticulturae10060562",
pages = "562"
}

Popržen, T., Jevremović, S., Milošević, S., Đurić, M., Uzelac, B., Stanković, S.,& Radulović, O.. (2024). Antioxidative Response of Duckweed (Lemna minor L.) to Rhizosphere-Associated Pseudomonas Strains and Exogenous Indole-3-Acetic Acid. in Horticulturae
Basel: MDPI., 10(6), 562.
https://doi.org/10.3390/horticulturae10060562

Popržen T, Jevremović S, Milošević S, Đurić M, Uzelac B, Stanković S, Radulović O. Antioxidative Response of Duckweed (Lemna minor L.) to Rhizosphere-Associated Pseudomonas Strains and Exogenous Indole-3-Acetic Acid. in Horticulturae. 2024;10(6):562.
doi:10.3390/horticulturae10060562 .

Popržen, Tatjana, Jevremović, Slađana, Milošević, Snežana, Đurić, Marija, Uzelac, Branka, Stanković, Slaviša, Radulović, Olga, "Antioxidative Response of Duckweed (Lemna minor L.) to Rhizosphere-Associated Pseudomonas Strains and Exogenous Indole-3-Acetic Acid" in Horticulturae, 10, no. 6 (2024):562,
https://doi.org/10.3390/horticulturae10060562 . .

TY  - JOUR
AU  - Bjelica, Vukašin
AU  - Anđelković, Marko
AU  - Maričić, Marko
AU  - Tomović, Ljiljana
AU  - Bonnet, Xavier
AU  - Golubović, Ana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6756
AB  - When captured, many prey respond by biting the predator or struggling to get free.
However, one indirect escape option is death feigning (DF), in which complete
immobility supposedly “tricks” the predator into abandoning its meal. But lying
motionless and exposed to a predator is dangerous; therefore, individuals should
optimize DF occurrence and duration. We captured colour polymorphic dice snakes
(Natrix tessellata, Natricidae) (N = 271) in the field and measured two behavioural
responses: (1) the occurrence and duration of immobility/DF; (2) the number of
tongue-flicking sequences (TF). Tongue flicking is an essential component of a key
sensory mechanism to check the safety of the environment before attempting
escape. We experimentally assessed the relationship between these two behaviours
and the effects of phenotypic characteristic of snakes on the occurrence and duration
of immobility and of TF. Snake phenotype had multiple effects. Gravid
females avoided DF and displayed more TF sequences during the tests compared
to non-gravid females and males. Blotched snakes stayed immobile longer than
green and melanistic snakes. Larger individuals remained in DF for longer and
showed fewer TF sequences than smaller individuals. Snakes burdened with a
recent meal postponed fleeing and displayed more TF sequences than snakes without
food. Finally, snakes showing more TF sequences postponed fleeing, which
suggests that dice snakes assessed predatory risks and adapted escape behaviour to
their risk status. Future studies should examine how individuals estimate the appropriate
timing to shift from immobility to escape.
PB  - Hoboken: Wiley
T2  - Journal of Zoology
T1  - Tongue flicking heralds flight behaviour following passive antipredator displays in dice snakes
IS  - 4
VL  - 322
DO  - 10.1111/jzo.13150
SP  - 364
EP  - 374
ER  - 

@article{
author = "Bjelica, Vukašin and Anđelković, Marko and Maričić, Marko and Tomović, Ljiljana and Bonnet, Xavier and Golubović, Ana",
year = "2024",
abstract = "When captured, many prey respond by biting the predator or struggling to get free.
However, one indirect escape option is death feigning (DF), in which complete
immobility supposedly “tricks” the predator into abandoning its meal. But lying
motionless and exposed to a predator is dangerous; therefore, individuals should
optimize DF occurrence and duration. We captured colour polymorphic dice snakes
(Natrix tessellata, Natricidae) (N = 271) in the field and measured two behavioural
responses: (1) the occurrence and duration of immobility/DF; (2) the number of
tongue-flicking sequences (TF). Tongue flicking is an essential component of a key
sensory mechanism to check the safety of the environment before attempting
escape. We experimentally assessed the relationship between these two behaviours
and the effects of phenotypic characteristic of snakes on the occurrence and duration
of immobility and of TF. Snake phenotype had multiple effects. Gravid
females avoided DF and displayed more TF sequences during the tests compared
to non-gravid females and males. Blotched snakes stayed immobile longer than
green and melanistic snakes. Larger individuals remained in DF for longer and
showed fewer TF sequences than smaller individuals. Snakes burdened with a
recent meal postponed fleeing and displayed more TF sequences than snakes without
food. Finally, snakes showing more TF sequences postponed fleeing, which
suggests that dice snakes assessed predatory risks and adapted escape behaviour to
their risk status. Future studies should examine how individuals estimate the appropriate
timing to shift from immobility to escape.",
publisher = "Hoboken: Wiley",
journal = "Journal of Zoology",
title = "Tongue flicking heralds flight behaviour following passive antipredator displays in dice snakes",
number = "4",
volume = "322",
doi = "10.1111/jzo.13150",
pages = "364-374"
}

Bjelica, V., Anđelković, M., Maričić, M., Tomović, L., Bonnet, X.,& Golubović, A.. (2024). Tongue flicking heralds flight behaviour following passive antipredator displays in dice snakes. in Journal of Zoology
Hoboken: Wiley., 322(4), 364-374.
https://doi.org/10.1111/jzo.13150

Bjelica V, Anđelković M, Maričić M, Tomović L, Bonnet X, Golubović A. Tongue flicking heralds flight behaviour following passive antipredator displays in dice snakes. in Journal of Zoology. 2024;322(4):364-374.
doi:10.1111/jzo.13150 .

Bjelica, Vukašin, Anđelković, Marko, Maričić, Marko, Tomović, Ljiljana, Bonnet, Xavier, Golubović, Ana, "Tongue flicking heralds flight behaviour following passive antipredator displays in dice snakes" in Journal of Zoology, 322, no. 4 (2024):364-374,
https://doi.org/10.1111/jzo.13150 . .

TY  - JOUR
AU  - Aničić, Neda
AU  - Mišić, Danijela
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6552
AB  - The genus Nepeta, belonging to the Lamiaceae family, encompasses a diverse group of plants with significant
biological activities attributed mainly to their iridoid compounds. This review provides a comprehensive analysis
of recent research on iridoid biosynthesis, regulation, and evolutionary aspects within the Nepeta genus. The biological
activities of Nepeta species, including repellent, phytotoxic, antimicrobial, and cytotoxic effects, have been extensively
investigated, highlighting the potential applications of iridoids. Over the past decade, significant progress has been
made in elucidating the molecular basis of iridoid biosynthesis and regulation, thanks to advancements in transcriptomics,
genomics, and metabolomics. The presence of distinct chemotype groups within Nepeta has been revealed,
characterized by their ability to produce both iridoid aglycones (nepetalactones) and glycosylated iridoids (IAs and
IGs), exclusively produce IGs, or lack iridoids. The identification of key enzymes involved in iridoid biosynthesis, such
as geraniol synthase (GES) and iridoid synthase (ISY), has played a crucial role in understanding the pathway. Furthermore,
the evolutionary aspects of the iridoid biosynthesis loss in some of the Nepeta taxa, and the association of iridoid
presence and content with the expression levels of specific genes, have been investigated. However, several areas remain
to be explored, including the final steps of iridoid aglycones biosynthetic branch, the production of iridoid glucosides,
the role of transcription factors in fine-tuning of iridoid biosynthesis, and the intricate interplay between biosynthetic
enzymes. Continued research in these areas will deepen our understanding of iridoid metabolism in Nepeta and unlock
their full potential in various fields, including pharmaceuticals, agriculture, and natural product-based industries.
PB  - Novi Sad, Serbia: Biologia Serbica
T2  - Biologia Serbica
T1  - Unveiling the evolution of iridoid biosynthesis in the genus Nepeta: a mini review
VL  - 46
DO  - 10.5281/zenodo.10606764
ER  - 

@article{
author = "Aničić, Neda and Mišić, Danijela",
year = "2024",
abstract = "The genus Nepeta, belonging to the Lamiaceae family, encompasses a diverse group of plants with significant
biological activities attributed mainly to their iridoid compounds. This review provides a comprehensive analysis
of recent research on iridoid biosynthesis, regulation, and evolutionary aspects within the Nepeta genus. The biological
activities of Nepeta species, including repellent, phytotoxic, antimicrobial, and cytotoxic effects, have been extensively
investigated, highlighting the potential applications of iridoids. Over the past decade, significant progress has been
made in elucidating the molecular basis of iridoid biosynthesis and regulation, thanks to advancements in transcriptomics,
genomics, and metabolomics. The presence of distinct chemotype groups within Nepeta has been revealed,
characterized by their ability to produce both iridoid aglycones (nepetalactones) and glycosylated iridoids (IAs and
IGs), exclusively produce IGs, or lack iridoids. The identification of key enzymes involved in iridoid biosynthesis, such
as geraniol synthase (GES) and iridoid synthase (ISY), has played a crucial role in understanding the pathway. Furthermore,
the evolutionary aspects of the iridoid biosynthesis loss in some of the Nepeta taxa, and the association of iridoid
presence and content with the expression levels of specific genes, have been investigated. However, several areas remain
to be explored, including the final steps of iridoid aglycones biosynthetic branch, the production of iridoid glucosides,
the role of transcription factors in fine-tuning of iridoid biosynthesis, and the intricate interplay between biosynthetic
enzymes. Continued research in these areas will deepen our understanding of iridoid metabolism in Nepeta and unlock
their full potential in various fields, including pharmaceuticals, agriculture, and natural product-based industries.",
publisher = "Novi Sad, Serbia: Biologia Serbica",
journal = "Biologia Serbica",
title = "Unveiling the evolution of iridoid biosynthesis in the genus Nepeta: a mini review",
volume = "46",
doi = "10.5281/zenodo.10606764"
}

Aničić, N.,& Mišić, D.. (2024). Unveiling the evolution of iridoid biosynthesis in the genus Nepeta: a mini review. in Biologia Serbica
Novi Sad, Serbia: Biologia Serbica., 46.
https://doi.org/10.5281/zenodo.10606764

Aničić N, Mišić D. Unveiling the evolution of iridoid biosynthesis in the genus Nepeta: a mini review. in Biologia Serbica. 2024;46.
doi:10.5281/zenodo.10606764 .

Aničić, Neda, Mišić, Danijela, "Unveiling the evolution of iridoid biosynthesis in the genus Nepeta: a mini review" in Biologia Serbica, 46 (2024),
https://doi.org/10.5281/zenodo.10606764 . .

TY  - THES
AU  - Erić, Pavle
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6797
AB  - Mitohondrijska DNK (mtDNK) kodira gene uključene u fundamentalne biološke procese ćelijske respiracije i proizvodnje energije. Mitohondrijski genom je haploidan, nasleđuje se uglavnom materinski, gotovo nikad se ne rekombinuje i odlikuje ga relativno mala efektivna veličina populacije. Zbog navedenih odlika mtDNK, uslovi koji predviđaju postojanje i održavanje adaptivne varijabilnosti mtDNK unutar populacija su restriktivni.
Analiziran je adaptivni značaj simpatričke mtDNK varijabilnosti, prisustvo mito-jedarnih interakcija, kao i selekcioni mehanizmi koji je održavaju: polno specifična selekcija, kao i selekcija u varijabilnim sredinskim uslovima. Korišćene su dve široko zastupljene palearktičke vrste roda Drosophila (iz Obscura grupe vrsta), D. subobscura i D. obscura. Prva vrsta je već ustaljeni model u ovom vidu evoluciono genetičkih istraživanja, i odlikuje se prisustvom dva učestala haplotipa u svim populacijama. Druga vrsta je kao model razvijena u ovoj disertaciji. Određena je njena genetička varijabilnost i izučena populaciona istorija kod nekoliko evropskih populacija. Izabrane su linije reprezentativnih haplotipova obe vrste i povratnim ukrštanjima su konstruisane eksperimentalne linije sa svim kombinacijama simpatričkih mtDNK haplotipova i jedarnih pozadina (nuDNK). Na dve eksperimentalne postavke koje su podrazumevale razlike u temperaturi i svetlosnom režimu analizirane su komponente životne istorije svih konstruisanih linija: otpornost na desikaciju, dužina razvića, preadultna vijabilnost, kao i procenat mužjaka (odnos polova).
Rezultati su pokazali postojanje značajne genetičke varijabilnosti kako unutar tako i između populacija D. obscura. Kod ove vrste je uočena geografska struktuiranost i smanjenje genetičkog diverziteta u pravcu od zapada ka istoku. Pokazan je adaptivni značaj simpatričke mtDNK varijabilnosti obe vrste. Kod D. subobscura je on bio prisutan na nivou dva glavna haplotipa jedino za vijabilitet, a kod ostalih komponenti na nivou pretpostavljenih dodatnih nukleotidnih razlika karakterističnih za pojedinačne linije. Pokazano je da su mito-jedarni genotipovi primarne jedinice na koje deluje prirodna selekcija, a efekat ovih interakcija na fitnes je bio izraženiji kod D. obscura. Pečat polno specifične selekcije nije zabeležen kod D. subobscura, dok je kod D. obscura efekat ovog vida balansne selekcije bio umeren. Naposletku, eksperimenti su pokazali umeren efekat interakcija sredine i mito-jedarnog genotipa na fitnes kod D. subobscura, dok su kod D. obscura pokazali jači uticaj ovog vida balansne selekcije. Uz to kod D. obscura primećen je i efekat interakcija samog mitohondrijskog haplotipa i sredinskih faktora na fitnes jedinki. Posmatrajući obe vrste, analizirana sredinska varijabilnost se pokazala važnijom u održavanju mtDNK varijabilnosti u poređenju sa polno specifičnom selekcijom.
Upotreba dve vrste sa različitim obrascima mtDNK varijabilnosti omogućila je uvid u adaptivne razlike na barem dva različita stupnja funkcionalne povezanosti mitohondrijskog i jedarnog genoma. Mali je broj eksperimentalnih radova koji ispituju mehanizme održavanja simpatričke varijabilnosti mtDNK, a rezultati ove studije umnogome doprinose njihovom boljem razumevanju.
AB  - Mitochondrial DNA (mtDNA) encodes genes involved in fundamental biological processes of cellular respiration and energy production. The mitochondrial genome is haploid, usually maternally inherited, rarely undergoes recombination, and is characterised by a relatively small effective population size. Due to these characteristics of mtDNA, conditions predicting the existence and maintenance of adaptive mtDNA variation within populations are restrictive.
The adaptive significance of sympatric mtDNA variability, the presence of mito-nuclear interactions, and selective mechanisms maintaining it were analysed, including sex-specific selection and selection in variable environmental conditions. Two widely distributed Palearctic species from the Drosophila genus (Obscura group species), D. subobscura and D. obscura, were used. The first species, a well-established model in evolutionary genetic research, features two common haplotypes in all populations. The second species was developed as a model in this dissertation. Genetic variability and population history of several European populations were determined as part of this thesis. Lines of representative haplotypes of both species were selected, and experimental lines with all combinations of sympatric mtDNA haplotypes and nuclear backgrounds (nuDNA) were constructed through backcrossing. All constructed lines of both species were subjected to life history experiments: desiccation resistance, developmental time, preadult viability, and sex ratio. Life history assays were conducted with two experimental setups involving differences in temperature and light regime.
The results showed significant genetic variability both within and between populations of D. obscura. In this species, geographic structuring and a reduction in genetic diversity from west to east was observed. The adaptive significance of sympatric mtDNA variation was demonstrated in both species. In D. subobscura, this significance was not present at the level of two main haplotypes except in viability experiment, and in other components it was assumed to involve additional, line specific nucleotide differences. Mito-nuclear genotypes were identified as primary units affected by natural selection, with a more pronounced effect of these interactions on fitness observed in D. obscura. Regarding sex-specific selection, its impact was not observed in D. subobscura, while in D. obscura, the effect of this type of balancing selection was moderate. Finally, experiments showed a moderate effect of interactions between the environment and mito-nuclear genotype on fitness in D. subobscura, while in D. obscura showed a stronger influence of this type of balancing selection. Additionally, in D. obscura, an effect of interactions between the mitochondrial haplotype itself and environmental factors on the fitness of individuals was observed. Considering both species, environmental variability proved more important in maintaining mtDNA variation compared to sex-specific selection.
The use of two species with different patterns of mtDNA variability provided insight into adaptive differences at least two different levels of functional connectivity between the mitochondrial and nuclear genomes. Few experimental studies have examined the mechanisms sustaining sympatric mtDNA variability, and the results of this study significantly contribute to a better understanding of these mechanisms.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Adaptivni značaj unutarpopulacione varijabilnosti mitohondrijskog genoma Drosophila subobscura i D. obscura
T1  - Adaptive significance of intrapopulational mitochondrial DNA variation in Drosophila subobscura and D. obscura
SP  - 1
EP  - 156
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6797
ER  - 

@phdthesis{
author = "Erić, Pavle",
year = "2024",
abstract = "Mitohondrijska DNK (mtDNK) kodira gene uključene u fundamentalne biološke procese ćelijske respiracije i proizvodnje energije. Mitohondrijski genom je haploidan, nasleđuje se uglavnom materinski, gotovo nikad se ne rekombinuje i odlikuje ga relativno mala efektivna veličina populacije. Zbog navedenih odlika mtDNK, uslovi koji predviđaju postojanje i održavanje adaptivne varijabilnosti mtDNK unutar populacija su restriktivni.
Analiziran je adaptivni značaj simpatričke mtDNK varijabilnosti, prisustvo mito-jedarnih interakcija, kao i selekcioni mehanizmi koji je održavaju: polno specifična selekcija, kao i selekcija u varijabilnim sredinskim uslovima. Korišćene su dve široko zastupljene palearktičke vrste roda Drosophila (iz Obscura grupe vrsta), D. subobscura i D. obscura. Prva vrsta je već ustaljeni model u ovom vidu evoluciono genetičkih istraživanja, i odlikuje se prisustvom dva učestala haplotipa u svim populacijama. Druga vrsta je kao model razvijena u ovoj disertaciji. Određena je njena genetička varijabilnost i izučena populaciona istorija kod nekoliko evropskih populacija. Izabrane su linije reprezentativnih haplotipova obe vrste i povratnim ukrštanjima su konstruisane eksperimentalne linije sa svim kombinacijama simpatričkih mtDNK haplotipova i jedarnih pozadina (nuDNK). Na dve eksperimentalne postavke koje su podrazumevale razlike u temperaturi i svetlosnom režimu analizirane su komponente životne istorije svih konstruisanih linija: otpornost na desikaciju, dužina razvića, preadultna vijabilnost, kao i procenat mužjaka (odnos polova).
Rezultati su pokazali postojanje značajne genetičke varijabilnosti kako unutar tako i između populacija D. obscura. Kod ove vrste je uočena geografska struktuiranost i smanjenje genetičkog diverziteta u pravcu od zapada ka istoku. Pokazan je adaptivni značaj simpatričke mtDNK varijabilnosti obe vrste. Kod D. subobscura je on bio prisutan na nivou dva glavna haplotipa jedino za vijabilitet, a kod ostalih komponenti na nivou pretpostavljenih dodatnih nukleotidnih razlika karakterističnih za pojedinačne linije. Pokazano je da su mito-jedarni genotipovi primarne jedinice na koje deluje prirodna selekcija, a efekat ovih interakcija na fitnes je bio izraženiji kod D. obscura. Pečat polno specifične selekcije nije zabeležen kod D. subobscura, dok je kod D. obscura efekat ovog vida balansne selekcije bio umeren. Naposletku, eksperimenti su pokazali umeren efekat interakcija sredine i mito-jedarnog genotipa na fitnes kod D. subobscura, dok su kod D. obscura pokazali jači uticaj ovog vida balansne selekcije. Uz to kod D. obscura primećen je i efekat interakcija samog mitohondrijskog haplotipa i sredinskih faktora na fitnes jedinki. Posmatrajući obe vrste, analizirana sredinska varijabilnost se pokazala važnijom u održavanju mtDNK varijabilnosti u poređenju sa polno specifičnom selekcijom.
Upotreba dve vrste sa različitim obrascima mtDNK varijabilnosti omogućila je uvid u adaptivne razlike na barem dva različita stupnja funkcionalne povezanosti mitohondrijskog i jedarnog genoma. Mali je broj eksperimentalnih radova koji ispituju mehanizme održavanja simpatričke varijabilnosti mtDNK, a rezultati ove studije umnogome doprinose njihovom boljem razumevanju., Mitochondrial DNA (mtDNA) encodes genes involved in fundamental biological processes of cellular respiration and energy production. The mitochondrial genome is haploid, usually maternally inherited, rarely undergoes recombination, and is characterised by a relatively small effective population size. Due to these characteristics of mtDNA, conditions predicting the existence and maintenance of adaptive mtDNA variation within populations are restrictive.
The adaptive significance of sympatric mtDNA variability, the presence of mito-nuclear interactions, and selective mechanisms maintaining it were analysed, including sex-specific selection and selection in variable environmental conditions. Two widely distributed Palearctic species from the Drosophila genus (Obscura group species), D. subobscura and D. obscura, were used. The first species, a well-established model in evolutionary genetic research, features two common haplotypes in all populations. The second species was developed as a model in this dissertation. Genetic variability and population history of several European populations were determined as part of this thesis. Lines of representative haplotypes of both species were selected, and experimental lines with all combinations of sympatric mtDNA haplotypes and nuclear backgrounds (nuDNA) were constructed through backcrossing. All constructed lines of both species were subjected to life history experiments: desiccation resistance, developmental time, preadult viability, and sex ratio. Life history assays were conducted with two experimental setups involving differences in temperature and light regime.
The results showed significant genetic variability both within and between populations of D. obscura. In this species, geographic structuring and a reduction in genetic diversity from west to east was observed. The adaptive significance of sympatric mtDNA variation was demonstrated in both species. In D. subobscura, this significance was not present at the level of two main haplotypes except in viability experiment, and in other components it was assumed to involve additional, line specific nucleotide differences. Mito-nuclear genotypes were identified as primary units affected by natural selection, with a more pronounced effect of these interactions on fitness observed in D. obscura. Regarding sex-specific selection, its impact was not observed in D. subobscura, while in D. obscura, the effect of this type of balancing selection was moderate. Finally, experiments showed a moderate effect of interactions between the environment and mito-nuclear genotype on fitness in D. subobscura, while in D. obscura showed a stronger influence of this type of balancing selection. Additionally, in D. obscura, an effect of interactions between the mitochondrial haplotype itself and environmental factors on the fitness of individuals was observed. Considering both species, environmental variability proved more important in maintaining mtDNA variation compared to sex-specific selection.
The use of two species with different patterns of mtDNA variability provided insight into adaptive differences at least two different levels of functional connectivity between the mitochondrial and nuclear genomes. Few experimental studies have examined the mechanisms sustaining sympatric mtDNA variability, and the results of this study significantly contribute to a better understanding of these mechanisms.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Adaptivni značaj unutarpopulacione varijabilnosti mitohondrijskog genoma Drosophila subobscura i D. obscura, Adaptive significance of intrapopulational mitochondrial DNA variation in Drosophila subobscura and D. obscura",
pages = "1-156",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6797"
}

Erić, P.. (2024). Adaptivni značaj unutarpopulacione varijabilnosti mitohondrijskog genoma Drosophila subobscura i D. obscura. in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-156.
https://hdl.handle.net/21.15107/rcub_ibiss_6797

Erić P. Adaptivni značaj unutarpopulacione varijabilnosti mitohondrijskog genoma Drosophila subobscura i D. obscura. in University of Belgrade, Faculty of Biology. 2024;:1-156.
https://hdl.handle.net/21.15107/rcub_ibiss_6797 .

Erić, Pavle, "Adaptivni značaj unutarpopulacione varijabilnosti mitohondrijskog genoma Drosophila subobscura i D. obscura" in University of Belgrade, Faculty of Biology (2024):1-156,
https://hdl.handle.net/21.15107/rcub_ibiss_6797 .

TY  - DATA
AU  - Budečević, Sanja
AU  - Predojević, Dragana
AU  - Đorđević, Mirko
AU  - Vlajnić, Lea
AU  - Stojković, Oliver
AU  - Stojković, Biljana
AU  - Savković, Uroš
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6730
AB  - This file contains data for the morphological analyses (landmarks coordinates) of  four populations of seed beetle, Acanthoscelides obtectus exposed to sub-lethal doses of cypermethrin for ten generations in a laboratory evolution experiment. This file is in the .csv format and it contains one sheet and forty nine columns.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade
T1  - Data for Transgenerational sub-lethal pyrethroid exposure affects shape variation and fluctuating asymmetry in a seed beetle
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6730
ER  - 

@misc{
author = "Budečević, Sanja and Predojević, Dragana and Đorđević, Mirko and Vlajnić, Lea and Stojković, Oliver and Stojković, Biljana and Savković, Uroš",
year = "2024",
abstract = "This file contains data for the morphological analyses (landmarks coordinates) of  four populations of seed beetle, Acanthoscelides obtectus exposed to sub-lethal doses of cypermethrin for ten generations in a laboratory evolution experiment. This file is in the .csv format and it contains one sheet and forty nine columns.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade",
title = "Data for Transgenerational sub-lethal pyrethroid exposure affects shape variation and fluctuating asymmetry in a seed beetle",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6730"
}

Budečević, S., Predojević, D., Đorđević, M., Vlajnić, L., Stojković, O., Stojković, B.,& Savković, U.. (2024). Data for Transgenerational sub-lethal pyrethroid exposure affects shape variation and fluctuating asymmetry in a seed beetle. 
Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_6730

Budečević S, Predojević D, Đorđević M, Vlajnić L, Stojković O, Stojković B, Savković U. Data for Transgenerational sub-lethal pyrethroid exposure affects shape variation and fluctuating asymmetry in a seed beetle. 2024;.
https://hdl.handle.net/21.15107/rcub_ibiss_6730 .

Budečević, Sanja, Predojević, Dragana, Đorđević, Mirko, Vlajnić, Lea, Stojković, Oliver, Stojković, Biljana, Savković, Uroš, "Data for Transgenerational sub-lethal pyrethroid exposure affects shape variation and fluctuating asymmetry in a seed beetle" (2024),
https://hdl.handle.net/21.15107/rcub_ibiss_6730 .

TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Jevremović, Slađana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6713
AB  - Aesculus species are valuable ornamental plants, while their seeds are used as a source of health-promoting aescin. The somatic embryos (SE) of A. hippocastanum (horse chestnut) contain aescin in similar amounts as the seeds. Different pathways of propagation by tissue culture for several Aesculus species (A. hippocastanum, A. flava and A. carnea) have been investigated for few decades in our laboratory. Recently, an efficient protocol for somatic embryogenesis induction in stamen filaments culture has been developed. However, the frequency of embryogenic tissue (ET) induction varied markedly (0–50%) for the same genotypes from year to year. In A. flava, a significant increase (80–100%) in the frequency of ET induction was achieved by using liquid culture of friable calli. After the establishment of stable liquid cultures, lines with high ET proliferation and SE development were selected. Significant fresh weight increase (76–167-fold), with 256–669 newly developed SEs per 100 mg of initial inoculum, were achieved four weeks after plating on solid medium. In addition, most SEs (19–39%) reached the late torpedo and cotyledonary stage of development. SEs have high germination rate (80%), but conversion rates still need to be improved to enable sustained clonal propagation of elite specimens. The established protocols for ET induction and SE production represent a clean and safe technology for the in vitro production of Aesculus species, including alternative sources of aescin.
PB  - COST Action CA21157 COPYTREE
C3  - Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia
T1  - Recent advances in somatic embryogenesis induction in Aesculus species
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6713
ER  - 

@conference{
author = "Zdravković-Korać, Snežana and Ćalić, Dušica and Milojević, Jelena and Belić, Maja and Jevremović, Slađana",
year = "2024",
abstract = "Aesculus species are valuable ornamental plants, while their seeds are used as a source of health-promoting aescin. The somatic embryos (SE) of A. hippocastanum (horse chestnut) contain aescin in similar amounts as the seeds. Different pathways of propagation by tissue culture for several Aesculus species (A. hippocastanum, A. flava and A. carnea) have been investigated for few decades in our laboratory. Recently, an efficient protocol for somatic embryogenesis induction in stamen filaments culture has been developed. However, the frequency of embryogenic tissue (ET) induction varied markedly (0–50%) for the same genotypes from year to year. In A. flava, a significant increase (80–100%) in the frequency of ET induction was achieved by using liquid culture of friable calli. After the establishment of stable liquid cultures, lines with high ET proliferation and SE development were selected. Significant fresh weight increase (76–167-fold), with 256–669 newly developed SEs per 100 mg of initial inoculum, were achieved four weeks after plating on solid medium. In addition, most SEs (19–39%) reached the late torpedo and cotyledonary stage of development. SEs have high germination rate (80%), but conversion rates still need to be improved to enable sustained clonal propagation of elite specimens. The established protocols for ET induction and SE production represent a clean and safe technology for the in vitro production of Aesculus species, including alternative sources of aescin.",
publisher = "COST Action CA21157 COPYTREE",
journal = "Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia",
title = "Recent advances in somatic embryogenesis induction in Aesculus species",
pages = "38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6713"
}

Zdravković-Korać, S., Ćalić, D., Milojević, J., Belić, M.,& Jevremović, S.. (2024). Recent advances in somatic embryogenesis induction in Aesculus species. in Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia
COST Action CA21157 COPYTREE., 38.
https://hdl.handle.net/21.15107/rcub_ibiss_6713

Zdravković-Korać S, Ćalić D, Milojević J, Belić M, Jevremović S. Recent advances in somatic embryogenesis induction in Aesculus species. in Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia. 2024;:38.
https://hdl.handle.net/21.15107/rcub_ibiss_6713 .

Zdravković-Korać, Snežana, Ćalić, Dušica, Milojević, Jelena, Belić, Maja, Jevremović, Slađana, "Recent advances in somatic embryogenesis induction in Aesculus species" in Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia (2024):38,
https://hdl.handle.net/21.15107/rcub_ibiss_6713 .

TY  - CONF
AU  - Tovilović-Kovačević, Gordana
AU  - Ignjatović, Đurđica
AU  - Krstić-Milošević, Dijana
AU  - Tomić, Mirko
AU  - Ćosić, Tatjana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6712
AB  - Thymus pannonicus All. (Lamiaceae) is a perennial plant native to Central and Eastern Europe and Russia. The dried herb is traditionally used to treat mild respiratory and gastrointestinal illnesses, due to enrichment with monoterpenes and sesquiterpenes. Other secondary metabolites found in Thymus species, namely phenolic acids, flavonoids, flavone glucuronides, show numerous additional health-promoting effects (antioxidant, antidiabetic, anticancer). In this work, the antihepatoma activity of methanol extracts from in vitro shoot cultures of T. pannonicus citral chemotype on human HEPG2 hepatoma cells was evaluated. Two methanol extracts were investigated: E1 – derived from shoots grown on a medium without plant growth regulators and E2 – derived from shoots grown in the presence of the plant hormone indole-3-acetic acid (1mg/ml). Quantitative spectrophotometric analysis revealed that total phenolic and flavonoid contents were higher in E2 (540.4mg/g and 9.84mg/g, respectively) than in E1 (265.4mg/g and 6.52mg/g, respectively). Further analysis using HPLC identified rosmarinic acid (RA) as the dominant phenolic acid in both extracts, with E2 containing a slightly higher amount of RA than E1. The extracts reduced growth of HEPG2 cells in a dose- and time-dependent manner with moderate IC50 values (IC50(E1)=81.1µg/ml; IC50(E2)=77.8µg/ml, MTT, 96h), as shown by cell viability tests. Similarly, RA, used as a control, suppressed the growth of HEPG2 cells with an IC50=30.8µM (MTT, 96h). Flow cytometry of propidium iodide-stained cells showed that T. pannonicus extracts and RA arrested HEPG2 cells in the G2/M phase of the cell cycle, while fluorimetric measurement of cells loaded with dihydrorhodamine revealed mild elevation of intracellular ROS content induced by both extracts after 48h of treatment. Considering high content of RA in both extracts and similar antiproliferative effect of RA, E1 and E2 on HEPG2 cells, it could be proposed that RA is the active compound responsible for moderate antihepatoma activity of the investigated T. pannonicus extracts
PB  - Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo
C3  - Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina
T1  - Antihepatoma activity of methanol extracts from Thymus pannonicus in vitro shoot cultures
SP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6712
ER  - 

@conference{
author = "Tovilović-Kovačević, Gordana and Ignjatović, Đurđica and Krstić-Milošević, Dijana and Tomić, Mirko and Ćosić, Tatjana",
year = "2024",
abstract = "Thymus pannonicus All. (Lamiaceae) is a perennial plant native to Central and Eastern Europe and Russia. The dried herb is traditionally used to treat mild respiratory and gastrointestinal illnesses, due to enrichment with monoterpenes and sesquiterpenes. Other secondary metabolites found in Thymus species, namely phenolic acids, flavonoids, flavone glucuronides, show numerous additional health-promoting effects (antioxidant, antidiabetic, anticancer). In this work, the antihepatoma activity of methanol extracts from in vitro shoot cultures of T. pannonicus citral chemotype on human HEPG2 hepatoma cells was evaluated. Two methanol extracts were investigated: E1 – derived from shoots grown on a medium without plant growth regulators and E2 – derived from shoots grown in the presence of the plant hormone indole-3-acetic acid (1mg/ml). Quantitative spectrophotometric analysis revealed that total phenolic and flavonoid contents were higher in E2 (540.4mg/g and 9.84mg/g, respectively) than in E1 (265.4mg/g and 6.52mg/g, respectively). Further analysis using HPLC identified rosmarinic acid (RA) as the dominant phenolic acid in both extracts, with E2 containing a slightly higher amount of RA than E1. The extracts reduced growth of HEPG2 cells in a dose- and time-dependent manner with moderate IC50 values (IC50(E1)=81.1µg/ml; IC50(E2)=77.8µg/ml, MTT, 96h), as shown by cell viability tests. Similarly, RA, used as a control, suppressed the growth of HEPG2 cells with an IC50=30.8µM (MTT, 96h). Flow cytometry of propidium iodide-stained cells showed that T. pannonicus extracts and RA arrested HEPG2 cells in the G2/M phase of the cell cycle, while fluorimetric measurement of cells loaded with dihydrorhodamine revealed mild elevation of intracellular ROS content induced by both extracts after 48h of treatment. Considering high content of RA in both extracts and similar antiproliferative effect of RA, E1 and E2 on HEPG2 cells, it could be proposed that RA is the active compound responsible for moderate antihepatoma activity of the investigated T. pannonicus extracts",
publisher = "Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo",
journal = "Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina",
title = "Antihepatoma activity of methanol extracts from Thymus pannonicus in vitro shoot cultures",
pages = "27",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6712"
}

Tovilović-Kovačević, G., Ignjatović, Đ., Krstić-Milošević, D., Tomić, M.,& Ćosić, T.. (2024). Antihepatoma activity of methanol extracts from Thymus pannonicus in vitro shoot cultures. in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina
Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo., 27.
https://hdl.handle.net/21.15107/rcub_ibiss_6712

Tovilović-Kovačević G, Ignjatović Đ, Krstić-Milošević D, Tomić M, Ćosić T. Antihepatoma activity of methanol extracts from Thymus pannonicus in vitro shoot cultures. in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina. 2024;:27.
https://hdl.handle.net/21.15107/rcub_ibiss_6712 .

Tovilović-Kovačević, Gordana, Ignjatović, Đurđica, Krstić-Milošević, Dijana, Tomić, Mirko, Ćosić, Tatjana, "Antihepatoma activity of methanol extracts from Thymus pannonicus in vitro shoot cultures" in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina (2024):27,
https://hdl.handle.net/21.15107/rcub_ibiss_6712 .

TY  - CONF
AU  - Ignjatović, Đurđica
AU  - Nestorović, Nataša
AU  - Tomić, Mirko
AU  - Ristić, Nataša
AU  - Veličković, Nataša
AU  - Perović, Milka
AU  - Tovilović-Kovačević, Gordana
AU  - Manojlović-Stojanoski, Milica
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6711
AB  - During prenatal development, glucocorticoids control fetal growth and maturation of fetal tissues. Synthetic glucocorticoids are frequently administered to pregnant women at risk for preterm delivery. Despite their beneficial effects on lung maturation and fetal survival, their impact on the developing brain is less clear. Among postnatal factors that might have a profound effect on both the cognitive capacity and behavior of the offspring, high fructose consumption is particularly concerning in the young population. The present study aimed to investigate the effects of prenatal dexamethasone exposure additionally challenged with postnatal fructose overconsumption on anxiety and recognition memory performance in male Wistar rat offspring. Pregnant female rats were treated with three doses of dexamethasone (0.5 mg/kg/b.m.) during late gestation (16th-18th gestational day), corresponding to clinical human exposure. Male offspring were supplemented with a moderate dose of fructose, similar to those found in sugar-sweetened beverages (10% solution), from weaning till adulthood. Recognition memory and anxiety-like behavior were assessed using a novel object recognition test, and elevated plus maze, respectively. Hippocampal synaptic plasticity was estimated by the levels of GAP-43, synaptophysin, postsynaptic density protein 95 (PSD-95), and its activating phosphorylation (pPSD-95-Ser295). Prenatal dexamethasone treatment induced an anxiolytic-like effect, stimulation of exploratory behavior, and the improvement of recognition memory associated with an increase in GAP-43 protein level in the hippocampus. Fructose overconsumption after weaning did not modify the effects of prenatal glucocorticoid exposure in the applied experimental model, suggesting that fetal programming had a prevailing influence. According to our results, prenatal dexamethasone treatment may induce changes in reactions to novel situations in male Wistar rats which might represent advantageous fetal developmental adaptation to a new environment, while increased exploratory behavior, reduced anxiety, and improved ability to recognize novel objects could improve survival in an adverse postnatal environment.
PB  - Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo
C3  - Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina
T1  - Effects of prenatal dexamethasone treatment and moderate post-weaning fructose consumption on recognition memory in adult male Wistar rat offspring
SP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6711
ER  - 

@conference{
author = "Ignjatović, Đurđica and Nestorović, Nataša and Tomić, Mirko and Ristić, Nataša and Veličković, Nataša and Perović, Milka and Tovilović-Kovačević, Gordana and Manojlović-Stojanoski, Milica",
year = "2024",
abstract = "During prenatal development, glucocorticoids control fetal growth and maturation of fetal tissues. Synthetic glucocorticoids are frequently administered to pregnant women at risk for preterm delivery. Despite their beneficial effects on lung maturation and fetal survival, their impact on the developing brain is less clear. Among postnatal factors that might have a profound effect on both the cognitive capacity and behavior of the offspring, high fructose consumption is particularly concerning in the young population. The present study aimed to investigate the effects of prenatal dexamethasone exposure additionally challenged with postnatal fructose overconsumption on anxiety and recognition memory performance in male Wistar rat offspring. Pregnant female rats were treated with three doses of dexamethasone (0.5 mg/kg/b.m.) during late gestation (16th-18th gestational day), corresponding to clinical human exposure. Male offspring were supplemented with a moderate dose of fructose, similar to those found in sugar-sweetened beverages (10% solution), from weaning till adulthood. Recognition memory and anxiety-like behavior were assessed using a novel object recognition test, and elevated plus maze, respectively. Hippocampal synaptic plasticity was estimated by the levels of GAP-43, synaptophysin, postsynaptic density protein 95 (PSD-95), and its activating phosphorylation (pPSD-95-Ser295). Prenatal dexamethasone treatment induced an anxiolytic-like effect, stimulation of exploratory behavior, and the improvement of recognition memory associated with an increase in GAP-43 protein level in the hippocampus. Fructose overconsumption after weaning did not modify the effects of prenatal glucocorticoid exposure in the applied experimental model, suggesting that fetal programming had a prevailing influence. According to our results, prenatal dexamethasone treatment may induce changes in reactions to novel situations in male Wistar rats which might represent advantageous fetal developmental adaptation to a new environment, while increased exploratory behavior, reduced anxiety, and improved ability to recognize novel objects could improve survival in an adverse postnatal environment.",
publisher = "Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo",
journal = "Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina",
title = "Effects of prenatal dexamethasone treatment and moderate post-weaning fructose consumption on recognition memory in adult male Wistar rat offspring",
pages = "34",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6711"
}

Ignjatović, Đ., Nestorović, N., Tomić, M., Ristić, N., Veličković, N., Perović, M., Tovilović-Kovačević, G.,& Manojlović-Stojanoski, M.. (2024). Effects of prenatal dexamethasone treatment and moderate post-weaning fructose consumption on recognition memory in adult male Wistar rat offspring. in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina
Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo., 34.
https://hdl.handle.net/21.15107/rcub_ibiss_6711

Ignjatović Đ, Nestorović N, Tomić M, Ristić N, Veličković N, Perović M, Tovilović-Kovačević G, Manojlović-Stojanoski M. Effects of prenatal dexamethasone treatment and moderate post-weaning fructose consumption on recognition memory in adult male Wistar rat offspring. in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina. 2024;:34.
https://hdl.handle.net/21.15107/rcub_ibiss_6711 .

Ignjatović, Đurđica, Nestorović, Nataša, Tomić, Mirko, Ristić, Nataša, Veličković, Nataša, Perović, Milka, Tovilović-Kovačević, Gordana, Manojlović-Stojanoski, Milica, "Effects of prenatal dexamethasone treatment and moderate post-weaning fructose consumption on recognition memory in adult male Wistar rat offspring" in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina (2024):34,
https://hdl.handle.net/21.15107/rcub_ibiss_6711 .

TY  - CONF
AU  - Smailagić, Dijana
AU  - Dragišić Maksimović, Jelena
AU  - Marin, Marija
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stupar, Sofija
AU  - Stanišić, Mariana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6710
AB  - Phloretin is a phenolic compound from the apple that belongs to the group of dihydrochalcones. The genus Malus is a uniquely rich source of dihydrochalcones, accounting for about 97% of the total phenolic compounds in apple leaves and 10-20% of the dry weight of the leaf tissue. Due to its typical dihydrochalcone structure, phloretin is a highly reactive molecule that interacts efficiently with many biological macromolecules. Its reactivity leads to remarkable antioxidant and medicinal properties that have been extensively studied in mammals and humans. However, knowledge about the effects of phloretin on plant organisms is rather scarce. The phytotoxicity of phloretin in plants was first documented by our research group when phloretin was recognised as a potent allelochemical of the apple tree with deleterious effects in plant species outside the genus Malus. Research on the model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh] named the disruption of auxin homeostasis in the roots as the main mechanism of the phytotoxic effect of phloretin. The present study complements previous research with new insights into the ultrastructural changes in leaf mesophyll cells that underlie the growth arrest and decay of the aerial parts of Arabidopsis seedlings exposed to phloretin. The effects of phloretin are closely linked to the ultrastructural damage in the leaf mesophyll cells and range from mild effects with short-term treatment to severe effects with long-term treatment. Transmission electron microscopy revealed changes in the morphology of the cell wall, changes in thylakoid organisation, swelling and displacement of chloroplasts after short-term treatment. A significant decrease in the number of starch granules and chlorophyll content in the leaves reflected impaired chloroplast function leading to depletion of starch reserves, starvation of cells and recruitment of micro- and macroautophagic processes for self-maintenance leading to programmed cell death after long-term treatment with phloretin. These results confirm that the sites of action of phloretin in plant and animal cells do not coincide, confirming the safety of its potential application as a new bioherbicidal agent for humans and animals.
PB  - Turkey. Gebze,Turkey: Technical University
C3  - Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey
T1  - How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?
SP  - ID-739
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6710
ER  - 

@conference{
author = "Smailagić, Dijana and Dragišić Maksimović, Jelena and Marin, Marija and Banjac, Nevena and Ninković, Slavica and Stupar, Sofija and Stanišić, Mariana",
year = "2024",
abstract = "Phloretin is a phenolic compound from the apple that belongs to the group of dihydrochalcones. The genus Malus is a uniquely rich source of dihydrochalcones, accounting for about 97% of the total phenolic compounds in apple leaves and 10-20% of the dry weight of the leaf tissue. Due to its typical dihydrochalcone structure, phloretin is a highly reactive molecule that interacts efficiently with many biological macromolecules. Its reactivity leads to remarkable antioxidant and medicinal properties that have been extensively studied in mammals and humans. However, knowledge about the effects of phloretin on plant organisms is rather scarce. The phytotoxicity of phloretin in plants was first documented by our research group when phloretin was recognised as a potent allelochemical of the apple tree with deleterious effects in plant species outside the genus Malus. Research on the model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh] named the disruption of auxin homeostasis in the roots as the main mechanism of the phytotoxic effect of phloretin. The present study complements previous research with new insights into the ultrastructural changes in leaf mesophyll cells that underlie the growth arrest and decay of the aerial parts of Arabidopsis seedlings exposed to phloretin. The effects of phloretin are closely linked to the ultrastructural damage in the leaf mesophyll cells and range from mild effects with short-term treatment to severe effects with long-term treatment. Transmission electron microscopy revealed changes in the morphology of the cell wall, changes in thylakoid organisation, swelling and displacement of chloroplasts after short-term treatment. A significant decrease in the number of starch granules and chlorophyll content in the leaves reflected impaired chloroplast function leading to depletion of starch reserves, starvation of cells and recruitment of micro- and macroautophagic processes for self-maintenance leading to programmed cell death after long-term treatment with phloretin. These results confirm that the sites of action of phloretin in plant and animal cells do not coincide, confirming the safety of its potential application as a new bioherbicidal agent for humans and animals.",
publisher = "Turkey. Gebze,Turkey: Technical University",
journal = "Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey",
title = "How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?",
pages = "ID-739",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6710"
}

Smailagić, D., Dragišić Maksimović, J., Marin, M., Banjac, N., Ninković, S., Stupar, S.,& Stanišić, M.. (2024). How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?. in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey
Turkey. Gebze,Turkey: Technical University., ID-739.
https://hdl.handle.net/21.15107/rcub_ibiss_6710

Smailagić D, Dragišić Maksimović J, Marin M, Banjac N, Ninković S, Stupar S, Stanišić M. How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?. in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey. 2024;:ID-739.
https://hdl.handle.net/21.15107/rcub_ibiss_6710 .

Smailagić, Dijana, Dragišić Maksimović, Jelena, Marin, Marija, Banjac, Nevena, Ninković, Slavica, Stupar, Sofija, Stanišić, Mariana, "How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?" in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey (2024):ID-739,
https://hdl.handle.net/21.15107/rcub_ibiss_6710 .

TY  - JOUR
AU  - Bajić, Branka
AU  - Budinski, Ivana
AU  - Rajičić, Marija
AU  - Miljević, Milan
AU  - Rončević, Aleksa
AU  - Blagojević, Jelena
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6707
AB  - Schreiber’s bent-winged bat Miniopterus schreibersii (Chiroptera) is a widespread, cave-dwelling, regionally migrating species whose genetic diversity was studied throughout its distribution area using mitochondrial and nuclear markers. Previous studies revealed little to no structuring of populations and established Anatolia to be a single refugium during the last glacial maximum. The Balkans were well covered in these studies but usually lacked samples from Serbia. We sequenced the mitochondrial hypervariable region 1 (HV1) gene in M. schreibersii collected at seven sites in Serbia to assess their genetic relatedness to other European and Asia Minor populations and check whether the Balkans exhibit a higher genetic diversity than Western Europe due to its closeness to Anatolia. We recorded nine haplotypes from Serbia, six of which had not been previously reported, with a haplotype diversity of 0.585. The remaining three were shared with individuals from Portugal, Greece, and Turkey. A single most common haplotype was present throughout the species distribution range, pointing to a well-connected population and as indicated by the shape of the haplotype network, a common origin, and a sudden population expansion. Results complement existing data on M. schreibersii having a non-structured population, adding valuable new data from the Balkans supporting the previous hypothesis about its Anatolian origin.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - A first look at mitochondrial genetic diversity in Miniopterus schreibersii in Serbia
IS  - 1
VL  - 76
DO  - 10.2298/ABS240208006B
SP  - 103
EP  - 110
ER  - 

@article{
author = "Bajić, Branka and Budinski, Ivana and Rajičić, Marija and Miljević, Milan and Rončević, Aleksa and Blagojević, Jelena",
year = "2024",
abstract = "Schreiber’s bent-winged bat Miniopterus schreibersii (Chiroptera) is a widespread, cave-dwelling, regionally migrating species whose genetic diversity was studied throughout its distribution area using mitochondrial and nuclear markers. Previous studies revealed little to no structuring of populations and established Anatolia to be a single refugium during the last glacial maximum. The Balkans were well covered in these studies but usually lacked samples from Serbia. We sequenced the mitochondrial hypervariable region 1 (HV1) gene in M. schreibersii collected at seven sites in Serbia to assess their genetic relatedness to other European and Asia Minor populations and check whether the Balkans exhibit a higher genetic diversity than Western Europe due to its closeness to Anatolia. We recorded nine haplotypes from Serbia, six of which had not been previously reported, with a haplotype diversity of 0.585. The remaining three were shared with individuals from Portugal, Greece, and Turkey. A single most common haplotype was present throughout the species distribution range, pointing to a well-connected population and as indicated by the shape of the haplotype network, a common origin, and a sudden population expansion. Results complement existing data on M. schreibersii having a non-structured population, adding valuable new data from the Balkans supporting the previous hypothesis about its Anatolian origin.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "A first look at mitochondrial genetic diversity in Miniopterus schreibersii in Serbia",
number = "1",
volume = "76",
doi = "10.2298/ABS240208006B",
pages = "103-110"
}

Bajić, B., Budinski, I., Rajičić, M., Miljević, M., Rončević, A.,& Blagojević, J.. (2024). A first look at mitochondrial genetic diversity in Miniopterus schreibersii in Serbia. in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 76(1), 103-110.
https://doi.org/10.2298/ABS240208006B

Bajić B, Budinski I, Rajičić M, Miljević M, Rončević A, Blagojević J. A first look at mitochondrial genetic diversity in Miniopterus schreibersii in Serbia. in Archives of Biological Sciences. 2024;76(1):103-110.
doi:10.2298/ABS240208006B .

Bajić, Branka, Budinski, Ivana, Rajičić, Marija, Miljević, Milan, Rončević, Aleksa, Blagojević, Jelena, "A first look at mitochondrial genetic diversity in Miniopterus schreibersii in Serbia" in Archives of Biological Sciences, 76, no. 1 (2024):103-110,
https://doi.org/10.2298/ABS240208006B . .

TY  - JOUR
AU  - Lukovac, Tanja
AU  - Aleksić Hil, Olivera
AU  - Popović, Milka
AU  - Jovanović, Vitomir
AU  - Savić, Tatjana
AU  - Pavlović, Aleksandra
AU  - Pavlović, Dragan
PY  - 2024
UR  - https://www.mdpi.com/2227-9067/11/4/497
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6704
AB  - The current diagnosis of attention deficit hyperactivity disorder (ADHD) is based on history, clinical observation, and behavioral tests. There is a high demand to find biomarkers for the diagnosis of ADHD. The aim of this study is to analyze the serum profiles of several biomarkers, including homocysteine (Hcy), vitamin B12, vitamin D, ferritin, and iron, in a cohort of 133 male subjects (6.5–12.5 years), including 67 individuals with an ADHD diagnosis based on DSM-V criteria and 66 age-matched healthy boys (healthy controls, HC). Assessments for ADHD included the Iowa Conners’ Teacher Rating Scale (CPRS) and the ADHDT test, as well as cognitive assessments using the Wechsler Intelligence Scale for Children-Revised (WISC-R) and the TROG-2 language comprehension test. Hcy and iron were quantified using spectrophotometry, while vitamin B12 and total 25-hydroxy vitamin D levels were determined using an electrochemiluminescence immunoassay (ECLIA) and ferritin was measured using a particle-enhanced immunoturbidimetric assay. The results showed significantly increased Hcy levels and decreased vitamin B12 levels in ADHD patients compared to HCs. Multiple logistic regression analysis indicated that Hcy is a potential prognostic indicator for ADHD. These results suggest that elevated homocysteine and decreased vitamin B12 may serve as markers for the diagnosis and prognosis of ADHD.
PB  - Basel, Switzerland: Multidisciplinary Digital Publishing Institute (MDPI)
PB  - Basel: MDPI
T2  - Children (Basel)
T1  - Serum biomarker analysis in pediatric ADHD: Implications of homocysteine, vitamin B12, vitamin D, ferritin, and iron levels
IS  - 4
VL  - 11
DO  - 10.3390/children11040497
SP  - 497
ER  - 

@article{
author = "Lukovac, Tanja and Aleksić Hil, Olivera and Popović, Milka and Jovanović, Vitomir and Savić, Tatjana and Pavlović, Aleksandra and Pavlović, Dragan",
year = "2024",
abstract = "The current diagnosis of attention deficit hyperactivity disorder (ADHD) is based on history, clinical observation, and behavioral tests. There is a high demand to find biomarkers for the diagnosis of ADHD. The aim of this study is to analyze the serum profiles of several biomarkers, including homocysteine (Hcy), vitamin B12, vitamin D, ferritin, and iron, in a cohort of 133 male subjects (6.5–12.5 years), including 67 individuals with an ADHD diagnosis based on DSM-V criteria and 66 age-matched healthy boys (healthy controls, HC). Assessments for ADHD included the Iowa Conners’ Teacher Rating Scale (CPRS) and the ADHDT test, as well as cognitive assessments using the Wechsler Intelligence Scale for Children-Revised (WISC-R) and the TROG-2 language comprehension test. Hcy and iron were quantified using spectrophotometry, while vitamin B12 and total 25-hydroxy vitamin D levels were determined using an electrochemiluminescence immunoassay (ECLIA) and ferritin was measured using a particle-enhanced immunoturbidimetric assay. The results showed significantly increased Hcy levels and decreased vitamin B12 levels in ADHD patients compared to HCs. Multiple logistic regression analysis indicated that Hcy is a potential prognostic indicator for ADHD. These results suggest that elevated homocysteine and decreased vitamin B12 may serve as markers for the diagnosis and prognosis of ADHD.",
publisher = "Basel, Switzerland: Multidisciplinary Digital Publishing Institute (MDPI), Basel: MDPI",
journal = "Children (Basel)",
title = "Serum biomarker analysis in pediatric ADHD: Implications of homocysteine, vitamin B12, vitamin D, ferritin, and iron levels",
number = "4",
volume = "11",
doi = "10.3390/children11040497",
pages = "497"
}

Lukovac, T., Aleksić Hil, O., Popović, M., Jovanović, V., Savić, T., Pavlović, A.,& Pavlović, D.. (2024). Serum biomarker analysis in pediatric ADHD: Implications of homocysteine, vitamin B12, vitamin D, ferritin, and iron levels. in Children (Basel)
Basel, Switzerland: Multidisciplinary Digital Publishing Institute (MDPI)., 11(4), 497.
https://doi.org/10.3390/children11040497

Lukovac T, Aleksić Hil O, Popović M, Jovanović V, Savić T, Pavlović A, Pavlović D. Serum biomarker analysis in pediatric ADHD: Implications of homocysteine, vitamin B12, vitamin D, ferritin, and iron levels. in Children (Basel). 2024;11(4):497.
doi:10.3390/children11040497 .

Lukovac, Tanja, Aleksić Hil, Olivera, Popović, Milka, Jovanović, Vitomir, Savić, Tatjana, Pavlović, Aleksandra, Pavlović, Dragan, "Serum biomarker analysis in pediatric ADHD: Implications of homocysteine, vitamin B12, vitamin D, ferritin, and iron levels" in Children (Basel), 11, no. 4 (2024):497,
https://doi.org/10.3390/children11040497 . .

TY  - CONF
AU  - Brkljačić, Jelena
AU  - Jovanović, Mirna
AU  - Teofilović, Ana
AU  - Mićić, Bojana
AU  - Vratarić, Miloš
AU  - Milosavljević, Dragica
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Milivojević, Jasminka
AU  - Đorđević, Ana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6706
AB  - Healthy dietary habits with abundant fruit consumption may reduce prevalence and positively affect 
development and progression of various chronic diseases including obesity and insulin-resistance related 
diseases. Strawberry (Fragaria × ananassa Duch.) represent a valuable source of vitamins, minerals, fatty 
acids, dietary fiber, and various bioactive polyphenolic compounds such as anthocyanins and flavonoids 
which are known for their antioxidant and anti-inflammatory activities. In this study we evaluated the 
effect of newly introduced strawberry cultivar „Aprika” supplemented in the form of lyophilized powder, 
on obesity-related metabolic alterations in high-fat-diet fed mice. We hypothesized that chronic (8 weeks) 
supplementation of lyophilized strawberries in the amount comparable to two servings per day in humans, 
would  ameliorate  insulin  resistance  associated  with  obesity  in  C57BL/6  mice.  Furthermore,  we 
hypothesized that strawberry meal consumed at 7PM i.e. before active feeding period of mice (which 
corresponds to early breakfast in humans) would induce more pronounced beneficial effects compared to 
meal consumed at 7AM i.e. at the end of the active feeding period (which corresponds to late dinner in 
humans). High fat diet induced hyperglycaemia, hyperinsulinaemia, insulin resistance and obesity; and 
disturbed hepatic insulin signaling. Lyophilized strawberries, only when consumed before feeding period, 
reduced body weight gain and improved insulin sensitivity induced by high-fat diet (evidenced by lower 
area under the curve after an intraperitoneal GTT, reduced serum insulin levels and an insulin resistance 
index (IR-HOMA). Strawberry meals consumed after active feeding period had no such effect. However, 
strawberries, regardless of the time of consumption, restored protein level of Insulin receptor substrate 1 
(IRS1) in the liver and increased hepatic antioxidant enzymes level. In conclusion, strawberries improve 
insulin  sensitivity  and  raise  hepatic  antioxidant  capacity  in  mouse  model  of  diet  induced  obesity. 
Nevertheless, more beneficial effects were achieved when strawberries were consumed before active 
feeding period, as an early breakfast.
PB  - Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo
C3  - Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina
T1  - Dietary supplementation with liophilised strawberry improves insulin  sensitivity and redox status in mouse model of diet induced obesity
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6706
ER  - 

@conference{
author = "Brkljačić, Jelena and Jovanović, Mirna and Teofilović, Ana and Mićić, Bojana and Vratarić, Miloš and Milosavljević, Dragica and Dragišić Maksimović, Jelena and Maksimović, Vuk and Milivojević, Jasminka and Đorđević, Ana",
year = "2024",
abstract = "Healthy dietary habits with abundant fruit consumption may reduce prevalence and positively affect 
development and progression of various chronic diseases including obesity and insulin-resistance related 
diseases. Strawberry (Fragaria × ananassa Duch.) represent a valuable source of vitamins, minerals, fatty 
acids, dietary fiber, and various bioactive polyphenolic compounds such as anthocyanins and flavonoids 
which are known for their antioxidant and anti-inflammatory activities. In this study we evaluated the 
effect of newly introduced strawberry cultivar „Aprika” supplemented in the form of lyophilized powder, 
on obesity-related metabolic alterations in high-fat-diet fed mice. We hypothesized that chronic (8 weeks) 
supplementation of lyophilized strawberries in the amount comparable to two servings per day in humans, 
would  ameliorate  insulin  resistance  associated  with  obesity  in  C57BL/6  mice.  Furthermore,  we 
hypothesized that strawberry meal consumed at 7PM i.e. before active feeding period of mice (which 
corresponds to early breakfast in humans) would induce more pronounced beneficial effects compared to 
meal consumed at 7AM i.e. at the end of the active feeding period (which corresponds to late dinner in 
humans). High fat diet induced hyperglycaemia, hyperinsulinaemia, insulin resistance and obesity; and 
disturbed hepatic insulin signaling. Lyophilized strawberries, only when consumed before feeding period, 
reduced body weight gain and improved insulin sensitivity induced by high-fat diet (evidenced by lower 
area under the curve after an intraperitoneal GTT, reduced serum insulin levels and an insulin resistance 
index (IR-HOMA). Strawberry meals consumed after active feeding period had no such effect. However, 
strawberries, regardless of the time of consumption, restored protein level of Insulin receptor substrate 1 
(IRS1) in the liver and increased hepatic antioxidant enzymes level. In conclusion, strawberries improve 
insulin  sensitivity  and  raise  hepatic  antioxidant  capacity  in  mouse  model  of  diet  induced  obesity. 
Nevertheless, more beneficial effects were achieved when strawberries were consumed before active 
feeding period, as an early breakfast.",
publisher = "Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo",
journal = "Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina",
title = "Dietary supplementation with liophilised strawberry improves insulin  sensitivity and redox status in mouse model of diet induced obesity",
pages = "38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6706"
}

Brkljačić, J., Jovanović, M., Teofilović, A., Mićić, B., Vratarić, M., Milosavljević, D., Dragišić Maksimović, J., Maksimović, V., Milivojević, J.,& Đorđević, A.. (2024). Dietary supplementation with liophilised strawberry improves insulin  sensitivity and redox status in mouse model of diet induced obesity. in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina
Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo., 38.
https://hdl.handle.net/21.15107/rcub_ibiss_6706

Brkljačić J, Jovanović M, Teofilović A, Mićić B, Vratarić M, Milosavljević D, Dragišić Maksimović J, Maksimović V, Milivojević J, Đorđević A. Dietary supplementation with liophilised strawberry improves insulin  sensitivity and redox status in mouse model of diet induced obesity. in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina. 2024;:38.
https://hdl.handle.net/21.15107/rcub_ibiss_6706 .

Brkljačić, Jelena, Jovanović, Mirna, Teofilović, Ana, Mićić, Bojana, Vratarić, Miloš, Milosavljević, Dragica, Dragišić Maksimović, Jelena, Maksimović, Vuk, Milivojević, Jasminka, Đorđević, Ana, "Dietary supplementation with liophilised strawberry improves insulin  sensitivity and redox status in mouse model of diet induced obesity" in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina (2024):38,
https://hdl.handle.net/21.15107/rcub_ibiss_6706 .

TY  - JOUR
AU  - Ilić, Marija
AU  - Bugarski-Stanojević, Vanja
AU  - Jovanović, Bogdan
AU  - Stamenković, Gorana
AU  - Zorić, Katarina
AU  - Paunović, Momir
AU  - Crnobrnja-Isailović, Jelka
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6705
AB  - Simple Summary: Rana temporaria is one of the most widespread Palearctic brown frogs, with two
main genetic clades in Europe, geographic spatial pattern of which is insufficiently known. We
analyzed samples from the understudied western and central Balkans to evaluate the haplotype
diversity of widely used 16SrRNA and MT-CYTB mitochondrial gene sequences and to recognize
the contour of a possible contact zone between the main clades. The results revealed a suture
zone between theWestern and Eastern Clades in the central part of the Balkan Peninsula. Overall,
haplotype diversity in the western and central Balkans sample is high. Harboring both main genetic
clades of R. temporaria qualifies the Balkan Peninsula as another important center of species’ genetic
diversity, as well as rich in unique haplotypes.
Abstract: Rana temporaria is one of the most widespread Palearctic brown frogs. We aimed to
clarify distribution pattern of two main genetic clades in the understudied Balkan peninsula by
using 16SrRNA and MT-CYTB sequences, already widely applied in analyses of populations from
other parts of Europe, while focusing on the broad area along the Morava river (central Balkans)
as a known gap in the species distribution. Additionally, we were interested in revealing the
extent of haplotype diversity within the main genetic clades in the Balkans, particularly around
the supposed suture zone. The results revealed a suture zone between the Western and Eastern
Clades in the central part of the Balkan Peninsula. This indicated the existence of a historical
barrier between the Balkan Mountain Belt and geographically close mountains surrounding the
Vlasina Plateau (Rhodope/Serbian–Macedonian Massif). The overall observed haplotype diversity in
populations of R. temporaria from the Balkan Peninsula seems high. Harboring both main genetic
clades of R. temporaria qualifies the Balkan Peninsula as another important center of species’ genetic
diversity, as well as rich in unique haplotypes. This points out the necessity of applying conservation
measures focused on the common European frog populations and habitats in this part of the species’
distribution area.
PB  - Basel: MDPI
T2  - Animals
T1  - Phylogeographic Substructuring in the Southernmost Refugium of the European Common Frog Rana temporaria
IS  - 10
VL  - 14
DO  - 10.3390/ani14101430
SP  - 1430
ER  - 

@article{
author = "Ilić, Marija and Bugarski-Stanojević, Vanja and Jovanović, Bogdan and Stamenković, Gorana and Zorić, Katarina and Paunović, Momir and Crnobrnja-Isailović, Jelka",
year = "2024",
abstract = "Simple Summary: Rana temporaria is one of the most widespread Palearctic brown frogs, with two
main genetic clades in Europe, geographic spatial pattern of which is insufficiently known. We
analyzed samples from the understudied western and central Balkans to evaluate the haplotype
diversity of widely used 16SrRNA and MT-CYTB mitochondrial gene sequences and to recognize
the contour of a possible contact zone between the main clades. The results revealed a suture
zone between theWestern and Eastern Clades in the central part of the Balkan Peninsula. Overall,
haplotype diversity in the western and central Balkans sample is high. Harboring both main genetic
clades of R. temporaria qualifies the Balkan Peninsula as another important center of species’ genetic
diversity, as well as rich in unique haplotypes.
Abstract: Rana temporaria is one of the most widespread Palearctic brown frogs. We aimed to
clarify distribution pattern of two main genetic clades in the understudied Balkan peninsula by
using 16SrRNA and MT-CYTB sequences, already widely applied in analyses of populations from
other parts of Europe, while focusing on the broad area along the Morava river (central Balkans)
as a known gap in the species distribution. Additionally, we were interested in revealing the
extent of haplotype diversity within the main genetic clades in the Balkans, particularly around
the supposed suture zone. The results revealed a suture zone between the Western and Eastern
Clades in the central part of the Balkan Peninsula. This indicated the existence of a historical
barrier between the Balkan Mountain Belt and geographically close mountains surrounding the
Vlasina Plateau (Rhodope/Serbian–Macedonian Massif). The overall observed haplotype diversity in
populations of R. temporaria from the Balkan Peninsula seems high. Harboring both main genetic
clades of R. temporaria qualifies the Balkan Peninsula as another important center of species’ genetic
diversity, as well as rich in unique haplotypes. This points out the necessity of applying conservation
measures focused on the common European frog populations and habitats in this part of the species’
distribution area.",
publisher = "Basel: MDPI",
journal = "Animals",
title = "Phylogeographic Substructuring in the Southernmost Refugium of the European Common Frog Rana temporaria",
number = "10",
volume = "14",
doi = "10.3390/ani14101430",
pages = "1430"
}

Ilić, M., Bugarski-Stanojević, V., Jovanović, B., Stamenković, G., Zorić, K., Paunović, M.,& Crnobrnja-Isailović, J.. (2024). Phylogeographic Substructuring in the Southernmost Refugium of the European Common Frog Rana temporaria. in Animals
Basel: MDPI., 14(10), 1430.
https://doi.org/10.3390/ani14101430

Ilić M, Bugarski-Stanojević V, Jovanović B, Stamenković G, Zorić K, Paunović M, Crnobrnja-Isailović J. Phylogeographic Substructuring in the Southernmost Refugium of the European Common Frog Rana temporaria. in Animals. 2024;14(10):1430.
doi:10.3390/ani14101430 .

Ilić, Marija, Bugarski-Stanojević, Vanja, Jovanović, Bogdan, Stamenković, Gorana, Zorić, Katarina, Paunović, Momir, Crnobrnja-Isailović, Jelka, "Phylogeographic Substructuring in the Southernmost Refugium of the European Common Frog Rana temporaria" in Animals, 14, no. 10 (2024):1430,
https://doi.org/10.3390/ani14101430 . .

TY  - JOUR
AU  - Lukovac, Tanja
AU  - Aleksić Hil, Olivera
AU  - Popović, Milka
AU  - Savić, Tatjana
AU  - Pavlović, Aleksandra
AU  - Pavlović, Dragan
PY  - 2024
UR  - https://bmcneurol.biomedcentral.com/articles/10.1186/s12883-024-03563-w
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6703
AB  - Background: Although attention deficit hyperactivity disorder (ADHD) is a common neurodevelopmental disorder, its aetiology remains unclear. We aimed to establish a relationship between ADHD diagnosis and serum levels of glucose, free thyroxine (FT4), and thyroid stimulating hormone (TSH) in primary school aged boys.

Methods: In a cross-sectional study, we enrolled 133 participants aged 6.5-12.5 years, 67 of whom met DSM-5 criteria for ADHD and 66 healthy age-matched boys. The ADHDT test (ADHDT) was used to assess ADHD symptoms and the Wechsler Intelligence Scale for Children - Revised was used to exclude participants with cognitive deficits. The ADHD participants were tested using the Iowa Conners' Teacher Rating Scale.

Results: The ADHD participants had lower glucose levels, higher TSH values, and significantly lower FT4 values than the control group. The multiple logistic regression analysis showed that TSH is a parameter that is 2.7% more likely to occur in the ADHD group. We found a significant correlation between the TSH level and the symptoms of hyperactivity (r = 0.318, p = 0.009) and impulsivity (r = 0.275, p = 0.024) as well as between the glucose level and the symptoms of hyperactivity (r = 0.312, p = 0.010).

Conclusions: Certain ADHD symptoms may correlate with certain hormonal patterns. Our results suggest that the likelihood of suffering from ADHD was lower when FT4 levels were elevated. One biochemical parameter that was significantly and independently associated with the diagnosis of ADHD was the serum TSH level.

Trial registration: On June 26, 2018, at its VI session in 2018, the Ethics Committee of the Institute for Mental Health in Belgrade, Serbia, has considered and unanimously approved the conduct of the research, under the number 1704/1.
PB  - London, United Kingdom: BioMed Central Ltd.
T2  - BMC Neurology
T1  - Serum levels of glucose, thyroid stimulating hormone, and free thyroxine in boys diagnosed with attention deficit hyperactivity disorder: a cross-sectional pilot study
IS  - 1
VL  - 24
DO  - 10.1186/s12883-024-03563-w
SP  - 76
ER  - 

@article{
author = "Lukovac, Tanja and Aleksić Hil, Olivera and Popović, Milka and Savić, Tatjana and Pavlović, Aleksandra and Pavlović, Dragan",
year = "2024",
abstract = "Background: Although attention deficit hyperactivity disorder (ADHD) is a common neurodevelopmental disorder, its aetiology remains unclear. We aimed to establish a relationship between ADHD diagnosis and serum levels of glucose, free thyroxine (FT4), and thyroid stimulating hormone (TSH) in primary school aged boys.

Methods: In a cross-sectional study, we enrolled 133 participants aged 6.5-12.5 years, 67 of whom met DSM-5 criteria for ADHD and 66 healthy age-matched boys. The ADHDT test (ADHDT) was used to assess ADHD symptoms and the Wechsler Intelligence Scale for Children - Revised was used to exclude participants with cognitive deficits. The ADHD participants were tested using the Iowa Conners' Teacher Rating Scale.

Results: The ADHD participants had lower glucose levels, higher TSH values, and significantly lower FT4 values than the control group. The multiple logistic regression analysis showed that TSH is a parameter that is 2.7% more likely to occur in the ADHD group. We found a significant correlation between the TSH level and the symptoms of hyperactivity (r = 0.318, p = 0.009) and impulsivity (r = 0.275, p = 0.024) as well as between the glucose level and the symptoms of hyperactivity (r = 0.312, p = 0.010).

Conclusions: Certain ADHD symptoms may correlate with certain hormonal patterns. Our results suggest that the likelihood of suffering from ADHD was lower when FT4 levels were elevated. One biochemical parameter that was significantly and independently associated with the diagnosis of ADHD was the serum TSH level.

Trial registration: On June 26, 2018, at its VI session in 2018, the Ethics Committee of the Institute for Mental Health in Belgrade, Serbia, has considered and unanimously approved the conduct of the research, under the number 1704/1.",
publisher = "London, United Kingdom: BioMed Central Ltd.",
journal = "BMC Neurology",
title = "Serum levels of glucose, thyroid stimulating hormone, and free thyroxine in boys diagnosed with attention deficit hyperactivity disorder: a cross-sectional pilot study",
number = "1",
volume = "24",
doi = "10.1186/s12883-024-03563-w",
pages = "76"
}

Lukovac, T., Aleksić Hil, O., Popović, M., Savić, T., Pavlović, A.,& Pavlović, D.. (2024). Serum levels of glucose, thyroid stimulating hormone, and free thyroxine in boys diagnosed with attention deficit hyperactivity disorder: a cross-sectional pilot study. in BMC Neurology
London, United Kingdom: BioMed Central Ltd.., 24(1), 76.
https://doi.org/10.1186/s12883-024-03563-w

Lukovac T, Aleksić Hil O, Popović M, Savić T, Pavlović A, Pavlović D. Serum levels of glucose, thyroid stimulating hormone, and free thyroxine in boys diagnosed with attention deficit hyperactivity disorder: a cross-sectional pilot study. in BMC Neurology. 2024;24(1):76.
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Lukovac, Tanja, Aleksić Hil, Olivera, Popović, Milka, Savić, Tatjana, Pavlović, Aleksandra, Pavlović, Dragan, "Serum levels of glucose, thyroid stimulating hormone, and free thyroxine in boys diagnosed with attention deficit hyperactivity disorder: a cross-sectional pilot study" in BMC Neurology, 24, no. 1 (2024):76,
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TY  - JOUR
AU  - Šošić-Jurjević, Branka
AU  - Borković-Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Vlahović, Dragana
AU  - Miler, Marko
AU  - Cesar, Thais
AU  - Ajdžanović, Vladimir
AU  - Milenković, Dragan
AU  - Stellaard, Frans
AU  - Trifunović, Svetlana
AU  - Filipović, Branko
AU  - Dieter, Lutjohann
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6702
AB  - This study aimed to assess the antioxidant capacity of lemon flavonoid extract Eriomin®
(LE) and its impact on cholesterol metabolism in the context of healthy aging. We orally treated 24-
month-old male Wistar rats with an LE (40 mg/kg) suspended in 0.3 mL of sunflower oil. At the
same time, control groups received an equal volume of sunflower oil (CON) or remained untreated
(ICON) daily for 4 weeks. We examined LE’s effects on superoxide dismutase and catalase- and
glutathione-related enzyme activities, the concentration of lipid peroxides and protein carbonyls,
total oxidant status (TOS) and antioxidant status (TAS), and oxidative stress index (OSI) in the liver,
jejunum, and ileum. We also measured total cholesterol, its biosynthetic precursors (lanosterol,
lathosterol, desmosterol), its degradation products (bile acid precursors) in the serum, liver, jejunum, and ileum, and serum phytosterols (intestinal absorption markers). LE reduced TOS, TAS, and
OSI (p < 0.05) compared with control values, indicating its consistent antioxidant action in all examined organs. LE lowered hepatic desmosterol (p < 0.05) while also reducing 7α- and 24-hydroxycholesterol levels in the liver and ileum (p < 0.01). Serum cholesterol, hepatic gene expression, and the
immunostaining intensity of CYP7A1 were unchanged. In conclusion, LE exerted non-enzymatic
antioxidant effects and reduced cholesterol degradation, reducing its biosynthesis products, thereby
maintaining serum cholesterol levels.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats
IS  - 10
VL  - 25
DO  - 10.3390/ijms25105221
SP  - 5221
ER  - 

@article{
author = "Šošić-Jurjević, Branka and Borković-Mitić, Slavica and Pavlović, Slađan and Vlahović, Dragana and Miler, Marko and Cesar, Thais and Ajdžanović, Vladimir and Milenković, Dragan and Stellaard, Frans and Trifunović, Svetlana and Filipović, Branko and Dieter, Lutjohann",
year = "2024",
abstract = "This study aimed to assess the antioxidant capacity of lemon flavonoid extract Eriomin®
(LE) and its impact on cholesterol metabolism in the context of healthy aging. We orally treated 24-
month-old male Wistar rats with an LE (40 mg/kg) suspended in 0.3 mL of sunflower oil. At the
same time, control groups received an equal volume of sunflower oil (CON) or remained untreated
(ICON) daily for 4 weeks. We examined LE’s effects on superoxide dismutase and catalase- and
glutathione-related enzyme activities, the concentration of lipid peroxides and protein carbonyls,
total oxidant status (TOS) and antioxidant status (TAS), and oxidative stress index (OSI) in the liver,
jejunum, and ileum. We also measured total cholesterol, its biosynthetic precursors (lanosterol,
lathosterol, desmosterol), its degradation products (bile acid precursors) in the serum, liver, jejunum, and ileum, and serum phytosterols (intestinal absorption markers). LE reduced TOS, TAS, and
OSI (p < 0.05) compared with control values, indicating its consistent antioxidant action in all examined organs. LE lowered hepatic desmosterol (p < 0.05) while also reducing 7α- and 24-hydroxycholesterol levels in the liver and ileum (p < 0.01). Serum cholesterol, hepatic gene expression, and the
immunostaining intensity of CYP7A1 were unchanged. In conclusion, LE exerted non-enzymatic
antioxidant effects and reduced cholesterol degradation, reducing its biosynthesis products, thereby
maintaining serum cholesterol levels.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats",
number = "10",
volume = "25",
doi = "10.3390/ijms25105221",
pages = "5221"
}

Šošić-Jurjević, B., Borković-Mitić, S., Pavlović, S., Vlahović, D., Miler, M., Cesar, T., Ajdžanović, V., Milenković, D., Stellaard, F., Trifunović, S., Filipović, B.,& Dieter, L.. (2024). Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats. in International Journal of Molecular Sciences
Basel: MDPI., 25(10), 5221.
https://doi.org/10.3390/ijms25105221

Šošić-Jurjević B, Borković-Mitić S, Pavlović S, Vlahović D, Miler M, Cesar T, Ajdžanović V, Milenković D, Stellaard F, Trifunović S, Filipović B, Dieter L. Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats. in International Journal of Molecular Sciences. 2024;25(10):5221.
doi:10.3390/ijms25105221 .

Šošić-Jurjević, Branka, Borković-Mitić, Slavica, Pavlović, Slađan, Vlahović, Dragana, Miler, Marko, Cesar, Thais, Ajdžanović, Vladimir, Milenković, Dragan, Stellaard, Frans, Trifunović, Svetlana, Filipović, Branko, Dieter, Lutjohann, "Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats" in International Journal of Molecular Sciences, 25, no. 10 (2024):5221,
https://doi.org/10.3390/ijms25105221 . .