TY  - JOUR
AU  - Potrebić, Milica
AU  - Pavković, Željko
AU  - Puškaš, Nela
AU  - Pešić, Vesna
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fnbeh.2022.872628/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9113078
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4988
AB  - The fulfillment of belonging needs underlies a variety of behaviors. In order to understand how social needs unmet during maturation shape everyday life, we examined social motivation and cognition in peripubertal rats, as a rodent model of adolescence, subjected to social isolation (SI) during early and early-to-mid adolescence. The behavioral correlates of social orientation (social space preference), sociability (preference for social over non-social novelty), and social novelty preference (SNP) were examined in group-housed (GH) and single-housed (SH) rats in a 3-chamber test. The response to social odors was examined to gain insights into the developmental role of social odors in motivated social behavior. Differentiation between appetitive (number of visits/approaches) and consummatory (exploratory time) aspects of motivated social behavior was done to determine which facet of social motivation characterizes maturation when social needs are met and which aspect dominates when social needs are unsatisfied. The SI-sensitive parvalbumin-expressing interneurons (PVI) in the hippocampus were examined using immunohistochemistry. The main findings are the following: (1) in GH rats, the preference for social space is not evident regardless of animals' age, while sociability becomes apparent in mid-adolescence strictly through consummatory behavior, along with complete SNP (appetitive, consummatory); (2) SH promotes staying in a social chamber/space regardless of animals' age and produces an appetitive preference for it only in early-adolescent animals; (3) SH promotes sociability (appetitive, consummatory) regardless of the animals' age and prevents the SNP; (4) the preference for a social odor is displayed in all the groups through consummatory behavior, while appetitive behavior is evident only in SH rats; (5) the response to social odors does not commensurate directly to the response to conspecifics; (6) SH does not influence PVI in the hippocampus, except in the case of early-adolescence when a transient decrease in the dentate gyrus is observed. These results accentuate the developmental complexity of social motivation and cognition, and the power of SI in adolescence to infringe social maturation at different functional levels, promoting appetitive behavior toward peers overall but harming the interest for social novelty. The findings emphasize the importance of the fulfillment of basic social needs in the navigation through the social world.
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Behavioral Neuroscience
T1  - The Influence of Social Isolation on Social Orientation, Sociability, Social Novelty Preference, and Hippocampal Parvalbumin-Expressing Interneurons in Peripubertal Rats - Understanding the Importance of Meeting Social Needs in Adolescence.
VL  - 16
DO  - 10.3389/fnbeh.2022.872628
SP  - 872628
ER  - 

@article{
author = "Potrebić, Milica and Pavković, Željko and Puškaš, Nela and Pešić, Vesna",
year = "2022",
abstract = "The fulfillment of belonging needs underlies a variety of behaviors. In order to understand how social needs unmet during maturation shape everyday life, we examined social motivation and cognition in peripubertal rats, as a rodent model of adolescence, subjected to social isolation (SI) during early and early-to-mid adolescence. The behavioral correlates of social orientation (social space preference), sociability (preference for social over non-social novelty), and social novelty preference (SNP) were examined in group-housed (GH) and single-housed (SH) rats in a 3-chamber test. The response to social odors was examined to gain insights into the developmental role of social odors in motivated social behavior. Differentiation between appetitive (number of visits/approaches) and consummatory (exploratory time) aspects of motivated social behavior was done to determine which facet of social motivation characterizes maturation when social needs are met and which aspect dominates when social needs are unsatisfied. The SI-sensitive parvalbumin-expressing interneurons (PVI) in the hippocampus were examined using immunohistochemistry. The main findings are the following: (1) in GH rats, the preference for social space is not evident regardless of animals' age, while sociability becomes apparent in mid-adolescence strictly through consummatory behavior, along with complete SNP (appetitive, consummatory); (2) SH promotes staying in a social chamber/space regardless of animals' age and produces an appetitive preference for it only in early-adolescent animals; (3) SH promotes sociability (appetitive, consummatory) regardless of the animals' age and prevents the SNP; (4) the preference for a social odor is displayed in all the groups through consummatory behavior, while appetitive behavior is evident only in SH rats; (5) the response to social odors does not commensurate directly to the response to conspecifics; (6) SH does not influence PVI in the hippocampus, except in the case of early-adolescence when a transient decrease in the dentate gyrus is observed. These results accentuate the developmental complexity of social motivation and cognition, and the power of SI in adolescence to infringe social maturation at different functional levels, promoting appetitive behavior toward peers overall but harming the interest for social novelty. The findings emphasize the importance of the fulfillment of basic social needs in the navigation through the social world.",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Behavioral Neuroscience",
title = "The Influence of Social Isolation on Social Orientation, Sociability, Social Novelty Preference, and Hippocampal Parvalbumin-Expressing Interneurons in Peripubertal Rats - Understanding the Importance of Meeting Social Needs in Adolescence.",
volume = "16",
doi = "10.3389/fnbeh.2022.872628",
pages = "872628"
}

Potrebić, M., Pavković, Ž., Puškaš, N.,& Pešić, V.. (2022). The Influence of Social Isolation on Social Orientation, Sociability, Social Novelty Preference, and Hippocampal Parvalbumin-Expressing Interneurons in Peripubertal Rats - Understanding the Importance of Meeting Social Needs in Adolescence.. in Frontiers in Behavioral Neuroscience
Lausanne: Frontiers Media S.A.., 16, 872628.
https://doi.org/10.3389/fnbeh.2022.872628

Potrebić M, Pavković Ž, Puškaš N, Pešić V. The Influence of Social Isolation on Social Orientation, Sociability, Social Novelty Preference, and Hippocampal Parvalbumin-Expressing Interneurons in Peripubertal Rats - Understanding the Importance of Meeting Social Needs in Adolescence.. in Frontiers in Behavioral Neuroscience. 2022;16:872628.
doi:10.3389/fnbeh.2022.872628 .

Potrebić, Milica, Pavković, Željko, Puškaš, Nela, Pešić, Vesna, "The Influence of Social Isolation on Social Orientation, Sociability, Social Novelty Preference, and Hippocampal Parvalbumin-Expressing Interneurons in Peripubertal Rats - Understanding the Importance of Meeting Social Needs in Adolescence." in Frontiers in Behavioral Neuroscience, 16 (2022):872628,
https://doi.org/10.3389/fnbeh.2022.872628 . .

TY  - JOUR
AU  - Velimirović, Milica
AU  - Jevtić Dožudić, Gordana
AU  - Selaković, Vesna
AU  - Stojković, Tihomir
AU  - Puškaš, Nela
AU  - Zaletel, Ivan
AU  - Živković, Milica
AU  - Dragutinović, Vesna
AU  - Nikolić, Tatjana
AU  - Jelenković, Ankica
AU  - Đorović, Đorđe
AU  - Mirčić, Aleksandar
AU  - Petronijević, Nataša D.
PY  - 2018
UR  - https://www.hindawi.com/journals/omcl/2018/3273654/
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC5932460
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3167
AB  - Decreased blood flow in the brain leads to a rapid increase in reactive oxygen species (ROS). NADPH oxidase (NOX) is an enzyme family that has the physiological function to produce ROS. NOX2 and NOX4 overexpression is associated with aggravated ischemic injury, while NOX2/4-deficient mice had reduced stroke size. Dysregulation of matrix metalloproteinases (MMPs) contributes to tissue damage. The active form of vitamin D3 expresses neuroprotective, immunomodulatory, and anti-inflammatory effects in the CNS. The present study examines the effects of the vitamin D3 pretreatment on the oxidative stress parameters and the expression of NOX subunits, MMP9, microglial marker Iba1, and vitamin D receptor (VDR), in the cortex and hippocampus of Mongolian gerbils subjected to ten minutes of global cerebral ischemia, followed by 24 hours of reperfusion. The ischemia/reperfusion procedure has induced oxidative stress, changes in the expression of NOX2 subunits and MMP9 in the brain, and increased MMP9 activity in the serum of experimental animals. Pretreatment with vitamin D3 was especially effective on NOX2 subunits, MMP9, and the level of malondialdehyde and superoxide anion. These results outline the significance of the NOX and MMP9 investigation in brain ischemia and the importance of adequate vitamin D supplementation in ameliorating the injury caused by I/R.
T2  - Oxidative medicine and cellular longevity
T1  - Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia.
VL  - 2018
DO  - 10.1155/2018/3273654
SP  - 3273654
ER  - 

@article{
author = "Velimirović, Milica and Jevtić Dožudić, Gordana and Selaković, Vesna and Stojković, Tihomir and Puškaš, Nela and Zaletel, Ivan and Živković, Milica and Dragutinović, Vesna and Nikolić, Tatjana and Jelenković, Ankica and Đorović, Đorđe and Mirčić, Aleksandar and Petronijević, Nataša D.",
year = "2018",
abstract = "Decreased blood flow in the brain leads to a rapid increase in reactive oxygen species (ROS). NADPH oxidase (NOX) is an enzyme family that has the physiological function to produce ROS. NOX2 and NOX4 overexpression is associated with aggravated ischemic injury, while NOX2/4-deficient mice had reduced stroke size. Dysregulation of matrix metalloproteinases (MMPs) contributes to tissue damage. The active form of vitamin D3 expresses neuroprotective, immunomodulatory, and anti-inflammatory effects in the CNS. The present study examines the effects of the vitamin D3 pretreatment on the oxidative stress parameters and the expression of NOX subunits, MMP9, microglial marker Iba1, and vitamin D receptor (VDR), in the cortex and hippocampus of Mongolian gerbils subjected to ten minutes of global cerebral ischemia, followed by 24 hours of reperfusion. The ischemia/reperfusion procedure has induced oxidative stress, changes in the expression of NOX2 subunits and MMP9 in the brain, and increased MMP9 activity in the serum of experimental animals. Pretreatment with vitamin D3 was especially effective on NOX2 subunits, MMP9, and the level of malondialdehyde and superoxide anion. These results outline the significance of the NOX and MMP9 investigation in brain ischemia and the importance of adequate vitamin D supplementation in ameliorating the injury caused by I/R.",
journal = "Oxidative medicine and cellular longevity",
title = "Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia.",
volume = "2018",
doi = "10.1155/2018/3273654",
pages = "3273654"
}

Velimirović, M., Jevtić Dožudić, G., Selaković, V., Stojković, T., Puškaš, N., Zaletel, I., Živković, M., Dragutinović, V., Nikolić, T., Jelenković, A., Đorović, Đ., Mirčić, A.,& Petronijević, N. D.. (2018). Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia.. in Oxidative medicine and cellular longevity, 2018, 3273654.
https://doi.org/10.1155/2018/3273654

Velimirović M, Jevtić Dožudić G, Selaković V, Stojković T, Puškaš N, Zaletel I, Živković M, Dragutinović V, Nikolić T, Jelenković A, Đorović Đ, Mirčić A, Petronijević ND. Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia.. in Oxidative medicine and cellular longevity. 2018;2018:3273654.
doi:10.1155/2018/3273654 .

Velimirović, Milica, Jevtić Dožudić, Gordana, Selaković, Vesna, Stojković, Tihomir, Puškaš, Nela, Zaletel, Ivan, Živković, Milica, Dragutinović, Vesna, Nikolić, Tatjana, Jelenković, Ankica, Đorović, Đorđe, Mirčić, Aleksandar, Petronijević, Nataša D., "Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia." in Oxidative medicine and cellular longevity, 2018 (2018):3273654,
https://doi.org/10.1155/2018/3273654 . .

TY  - JOUR
AU  - Zaletel, Ivan
AU  - Schwirtlich, Marija
AU  - Perović, Milka
AU  - Jovanović, Mirna
AU  - Stevanović, Milena
AU  - Kanazir, Selma
AU  - Puškaš, Nela
PY  - 2018
UR  - http://www.medra.org/servlet/aliasResolver?alias=iospress&doi=10.3233/JAD-180277
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3149
AB  - Dysregulation of neurogenesis in the subgranular zone (SGZ) of the hippocampal dentate gyrus has been related to cognitive deficits and memory loss in neurodegenerative diseases, such as Alzheimer's disease (AD). Members of the B group of SOX transcription factors play critical roles in regulating neurogenesis in the embryonic and adult nervous system, including maintaining the multipotency, renewal, and cell fate decision of neural stem/progenitor cells. The aim of the present study was to evaluate the expression patterns of selected SOXB proteins in the SGZ, of 8-week-old male and female 5xFAD mice, which represent a transgenic model of AD with a severe and very early development of amyloid pathology. Immunohistochemical analysis showed a significant decrease in the number of cells expressing SOX1, SOX2, and SOX21 transcription factors within the SGZ of 5xFAD mice in comparison to their non-transgenic counterparts which coincidences with reduced number of doublecortin immunoreactive immature neurons found in Tg males. Despite observed changes in expressional pattern of examined SOXB proteins, the proliferative capacity evaluated by the number of Ki-67 immunoreactive cells remained unaffected in transgenic mice of both genders. Based on our results, we suggest that SOXB proteins might be considered as new biomarkers for the detection of early impairments in adult neurogenesis in different animal models or/and new targets in human regenerative medicine.
T2  - Journal of Alzheimer's Disease : JAD
T1  - Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors.
IS  - 3
VL  - 65
DO  - 10.3233/JAD-180277
SP  - 963
EP  - 976
ER  - 

@article{
author = "Zaletel, Ivan and Schwirtlich, Marija and Perović, Milka and Jovanović, Mirna and Stevanović, Milena and Kanazir, Selma and Puškaš, Nela",
year = "2018",
abstract = "Dysregulation of neurogenesis in the subgranular zone (SGZ) of the hippocampal dentate gyrus has been related to cognitive deficits and memory loss in neurodegenerative diseases, such as Alzheimer's disease (AD). Members of the B group of SOX transcription factors play critical roles in regulating neurogenesis in the embryonic and adult nervous system, including maintaining the multipotency, renewal, and cell fate decision of neural stem/progenitor cells. The aim of the present study was to evaluate the expression patterns of selected SOXB proteins in the SGZ, of 8-week-old male and female 5xFAD mice, which represent a transgenic model of AD with a severe and very early development of amyloid pathology. Immunohistochemical analysis showed a significant decrease in the number of cells expressing SOX1, SOX2, and SOX21 transcription factors within the SGZ of 5xFAD mice in comparison to their non-transgenic counterparts which coincidences with reduced number of doublecortin immunoreactive immature neurons found in Tg males. Despite observed changes in expressional pattern of examined SOXB proteins, the proliferative capacity evaluated by the number of Ki-67 immunoreactive cells remained unaffected in transgenic mice of both genders. Based on our results, we suggest that SOXB proteins might be considered as new biomarkers for the detection of early impairments in adult neurogenesis in different animal models or/and new targets in human regenerative medicine.",
journal = "Journal of Alzheimer's Disease : JAD",
title = "Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors.",
number = "3",
volume = "65",
doi = "10.3233/JAD-180277",
pages = "963-976"
}

Zaletel, I., Schwirtlich, M., Perović, M., Jovanović, M., Stevanović, M., Kanazir, S.,& Puškaš, N.. (2018). Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors.. in Journal of Alzheimer's Disease : JAD, 65(3), 963-976.
https://doi.org/10.3233/JAD-180277

Zaletel I, Schwirtlich M, Perović M, Jovanović M, Stevanović M, Kanazir S, Puškaš N. Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors.. in Journal of Alzheimer's Disease : JAD. 2018;65(3):963-976.
doi:10.3233/JAD-180277 .

Zaletel, Ivan, Schwirtlich, Marija, Perović, Milka, Jovanović, Mirna, Stevanović, Milena, Kanazir, Selma, Puškaš, Nela, "Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors." in Journal of Alzheimer's Disease : JAD, 65, no. 3 (2018):963-976,
https://doi.org/10.3233/JAD-180277 . .

TY  - JOUR
AU  - Tešić, Vesna
AU  - Perović, Milka
AU  - Zaletel, Ivan
AU  - Jovanović, Mirna
AU  - Puškaš, Nela
AU  - Ruždijić, Sabera
AU  - Kanazir, Selma
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0531556517302462
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2839
AB  - The administration of dexamethasone, a synthetic glucocorticoid receptor agonist, has been reported to modulate cognitive performance in both animals and humans. In the present study, we demonstrate the effects of a single high dose of dexamethasone on the expression and distribution of synaptic plasticity-related proteins, growth-associated protein-43 (GAP-43) and synaptophysin, in the hippocampus of 6-, 12-, 18- and 24-month-old rats. Acute dexamethasone treatment significantly altered the expression of GAP-43 at the posttranslational level by modulating the levels of phosphorylated GAP-43 and proteolytic GAP-43-3 fragment. The effect was the most pronounced in the hippocampi of the aged animals. The total GAP-43 protein was increased only in 24-month-old dexamethasone-treated animals, and was concomitant with a decrease in calpain-mediated proteolysis. Moreover, by introducing the gray level co-occurrence matrix method, a form of texture analysis, we were able to reveal the subtle differences in the expression pattern of both GAP-43 and synaptophysin in the hippocampal subfields that were not detected by Western blot analysis alone. Therefore, the current study demonstrates, through a novel combined approach, that dexamethasone treatment significantly affects both GAP-43 and synaptophysin protein expression in the hippocampus of aged rats.
T2  - Experimental Gerontology
T1  - A single high dose of dexamethasone increases GAP-43 and synaptophysin in the hippocampus of aged rats
VL  - 98
DO  - 10.1016/j.exger.2017.08.010
SP  - 62
EP  - 69
ER  - 

@article{
author = "Tešić, Vesna and Perović, Milka and Zaletel, Ivan and Jovanović, Mirna and Puškaš, Nela and Ruždijić, Sabera and Kanazir, Selma",
year = "2017",
abstract = "The administration of dexamethasone, a synthetic glucocorticoid receptor agonist, has been reported to modulate cognitive performance in both animals and humans. In the present study, we demonstrate the effects of a single high dose of dexamethasone on the expression and distribution of synaptic plasticity-related proteins, growth-associated protein-43 (GAP-43) and synaptophysin, in the hippocampus of 6-, 12-, 18- and 24-month-old rats. Acute dexamethasone treatment significantly altered the expression of GAP-43 at the posttranslational level by modulating the levels of phosphorylated GAP-43 and proteolytic GAP-43-3 fragment. The effect was the most pronounced in the hippocampi of the aged animals. The total GAP-43 protein was increased only in 24-month-old dexamethasone-treated animals, and was concomitant with a decrease in calpain-mediated proteolysis. Moreover, by introducing the gray level co-occurrence matrix method, a form of texture analysis, we were able to reveal the subtle differences in the expression pattern of both GAP-43 and synaptophysin in the hippocampal subfields that were not detected by Western blot analysis alone. Therefore, the current study demonstrates, through a novel combined approach, that dexamethasone treatment significantly affects both GAP-43 and synaptophysin protein expression in the hippocampus of aged rats.",
journal = "Experimental Gerontology",
title = "A single high dose of dexamethasone increases GAP-43 and synaptophysin in the hippocampus of aged rats",
volume = "98",
doi = "10.1016/j.exger.2017.08.010",
pages = "62-69"
}

Tešić, V., Perović, M., Zaletel, I., Jovanović, M., Puškaš, N., Ruždijić, S.,& Kanazir, S.. (2017). A single high dose of dexamethasone increases GAP-43 and synaptophysin in the hippocampus of aged rats. in Experimental Gerontology, 98, 62-69.
https://doi.org/10.1016/j.exger.2017.08.010

Tešić V, Perović M, Zaletel I, Jovanović M, Puškaš N, Ruždijić S, Kanazir S. A single high dose of dexamethasone increases GAP-43 and synaptophysin in the hippocampus of aged rats. in Experimental Gerontology. 2017;98:62-69.
doi:10.1016/j.exger.2017.08.010 .

Tešić, Vesna, Perović, Milka, Zaletel, Ivan, Jovanović, Mirna, Puškaš, Nela, Ruždijić, Sabera, Kanazir, Selma, "A single high dose of dexamethasone increases GAP-43 and synaptophysin in the hippocampus of aged rats" in Experimental Gerontology, 98 (2017):62-69,
https://doi.org/10.1016/j.exger.2017.08.010 . .