TY  - JOUR
AU  - Ajduković, Maja
AU  - Ukropina, Mirela
AU  - Cvijanović, Milena
AU  - Vučić, Tijana
AU  - Ivanović, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5579
AB  - Background: Amphibian skin has been studied for many decades, especially the metamorphic changes in
the skin of frogs. Less attention has been paid to salamander skin. Here, we describe changes in the skin
structure during postembryonic development in a salamandrid species, the Balkan crested newt Triturus
ivanbureschi.
Method: Using traditional histological techniques we examined the skin in the trunk region of three premetamorphic
larval stages (hatchling, mid larval and late larval) and two postmetamorphic stages (juvenile,
just after metamorphosis, and adult).
Results: In larval stages, skin consists only of the epidermis, which gradually develops from the single
epithelial cell layer in hatchlings, to a stratified epidermis with gland nests and characteristic Leydig cells at
the late larval stage. During metamorphosis, Leydig cells disappear, and the dermal layer develops. In
postmetamorphic stages, skin is differentiated on stratified epidermis and the dermis with well-developed
glands. Three types of glands were observed in the skin of the postmetamorphic stages: mucous, granular
and mixed. Gland composition appears to be stage- and sex-specific, with juveniles and adult female being
more similar to each other. In juveniles and adult female, there are a similar proportion of glands in both
dorsal and ventral skin, whereas in adult male granular glands dominated the dorsal skin, while mixed
glands dominated the ventral skin.
Conclusion: Our results provide a baseline for future comparative research of skin anatomy in salamanders.
PB  - Elsevier GmbH.
T2  - Annals of Anatomy - Anatomischer Anzeiger
T1  - Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae)
VL  - 249
DO  - 10.1016/j.aanat.2023.152097
SP  - 152097
ER  - 

@article{
author = "Ajduković, Maja and Ukropina, Mirela and Cvijanović, Milena and Vučić, Tijana and Ivanović, Ana",
year = "2023",
abstract = "Background: Amphibian skin has been studied for many decades, especially the metamorphic changes in
the skin of frogs. Less attention has been paid to salamander skin. Here, we describe changes in the skin
structure during postembryonic development in a salamandrid species, the Balkan crested newt Triturus
ivanbureschi.
Method: Using traditional histological techniques we examined the skin in the trunk region of three premetamorphic
larval stages (hatchling, mid larval and late larval) and two postmetamorphic stages (juvenile,
just after metamorphosis, and adult).
Results: In larval stages, skin consists only of the epidermis, which gradually develops from the single
epithelial cell layer in hatchlings, to a stratified epidermis with gland nests and characteristic Leydig cells at
the late larval stage. During metamorphosis, Leydig cells disappear, and the dermal layer develops. In
postmetamorphic stages, skin is differentiated on stratified epidermis and the dermis with well-developed
glands. Three types of glands were observed in the skin of the postmetamorphic stages: mucous, granular
and mixed. Gland composition appears to be stage- and sex-specific, with juveniles and adult female being
more similar to each other. In juveniles and adult female, there are a similar proportion of glands in both
dorsal and ventral skin, whereas in adult male granular glands dominated the dorsal skin, while mixed
glands dominated the ventral skin.
Conclusion: Our results provide a baseline for future comparative research of skin anatomy in salamanders.",
publisher = "Elsevier GmbH.",
journal = "Annals of Anatomy - Anatomischer Anzeiger",
title = "Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae)",
volume = "249",
doi = "10.1016/j.aanat.2023.152097",
pages = "152097"
}

Ajduković, M., Ukropina, M., Cvijanović, M., Vučić, T.,& Ivanović, A.. (2023). Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae). in Annals of Anatomy - Anatomischer Anzeiger
Elsevier GmbH.., 249, 152097.
https://doi.org/10.1016/j.aanat.2023.152097

Ajduković M, Ukropina M, Cvijanović M, Vučić T, Ivanović A. Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae). in Annals of Anatomy - Anatomischer Anzeiger. 2023;249:152097.
doi:10.1016/j.aanat.2023.152097 .

Ajduković, Maja, Ukropina, Mirela, Cvijanović, Milena, Vučić, Tijana, Ivanović, Ana, "Histological changes of the skin during postembryonic development of the crested newt Triturus ivanbureschi (Urodela, Salamandridae)" in Annals of Anatomy - Anatomischer Anzeiger, 249 (2023):152097,
https://doi.org/10.1016/j.aanat.2023.152097 . .

TY  - JOUR
AU  - Urošević, Aleksandar
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Scholtes, Stefan
AU  - Arntzen, Jan, W
AU  - Ivanović, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5817
AB  - Serially homologous structures may have complex patterns of regionalization and
morphological integration, influenced by developmental Hox gene expression and
functional constraints. The vertebral column, consisting of a number of repeated,
developmentally constrained, and highly integrated units—vertebrae—is such a
complex serially homologous structure. Functional diversification increases regionalization
and modularity of the vertebral column, particularly in mammals. For
salamanders, three concepts of regionalization of the vertebral column have been
proposed, recognizing one, two, or three presacral regions. Using three‐dimensional
geometric morphometrics on vertebra models acquired with microcomputerized
tomography scanning, we explored the covariation of vertebrae in four closely
related taxa of small‐bodied newts in the genus Lissotriton. The data were analyzed
by segmented linear regression to explore patterns of vertebral regionalization and
by a two‐block partial least squares method to test for morphological integration. All
taxa show a morphological shift posterior to the fifth trunk vertebra, which
corresponds to the two‐region concept. However, morphological integration is
found to be strongest in the mid‐trunk. Taken jointly, these results indicate a highly
integrated presacral vertebral column with a subtle two‐region differentiation. The
results are discussed in relation to specific functional requirements, developmental
and phylogenetic constraints, and specific requirements posed by a biphasic life
cycle and different locomotor modes (swimming vs. walking). Further research
should be conducted on different ontogenetic stages and closely related but
ecologically differentiated species.
PB  - John Wiley and Sons Inc
T2  - Journal of Experimental Zoology Part B - Molecular and Developmental Evolution
T1  - Regionalization and morphological integration in the vertebral column of Eurasian small‐bodied newts (Salamandridae: Lissotriton)
VL  - 2023
DO  - 10.1002/jez.b.23205
ER  - 

@article{
author = "Urošević, Aleksandar and Ajduković, Maja and Vučić, Tijana and Scholtes, Stefan and Arntzen, Jan, W and Ivanović, Ana",
year = "2023",
abstract = "Serially homologous structures may have complex patterns of regionalization and
morphological integration, influenced by developmental Hox gene expression and
functional constraints. The vertebral column, consisting of a number of repeated,
developmentally constrained, and highly integrated units—vertebrae—is such a
complex serially homologous structure. Functional diversification increases regionalization
and modularity of the vertebral column, particularly in mammals. For
salamanders, three concepts of regionalization of the vertebral column have been
proposed, recognizing one, two, or three presacral regions. Using three‐dimensional
geometric morphometrics on vertebra models acquired with microcomputerized
tomography scanning, we explored the covariation of vertebrae in four closely
related taxa of small‐bodied newts in the genus Lissotriton. The data were analyzed
by segmented linear regression to explore patterns of vertebral regionalization and
by a two‐block partial least squares method to test for morphological integration. All
taxa show a morphological shift posterior to the fifth trunk vertebra, which
corresponds to the two‐region concept. However, morphological integration is
found to be strongest in the mid‐trunk. Taken jointly, these results indicate a highly
integrated presacral vertebral column with a subtle two‐region differentiation. The
results are discussed in relation to specific functional requirements, developmental
and phylogenetic constraints, and specific requirements posed by a biphasic life
cycle and different locomotor modes (swimming vs. walking). Further research
should be conducted on different ontogenetic stages and closely related but
ecologically differentiated species.",
publisher = "John Wiley and Sons Inc",
journal = "Journal of Experimental Zoology Part B - Molecular and Developmental Evolution",
title = "Regionalization and morphological integration in the vertebral column of Eurasian small‐bodied newts (Salamandridae: Lissotriton)",
volume = "2023",
doi = "10.1002/jez.b.23205"
}

Urošević, A., Ajduković, M., Vučić, T., Scholtes, S., Arntzen, J. W.,& Ivanović, A.. (2023). Regionalization and morphological integration in the vertebral column of Eurasian small‐bodied newts (Salamandridae: Lissotriton). in Journal of Experimental Zoology Part B - Molecular and Developmental Evolution
John Wiley and Sons Inc., 2023.
https://doi.org/10.1002/jez.b.23205

Urošević A, Ajduković M, Vučić T, Scholtes S, Arntzen JW, Ivanović A. Regionalization and morphological integration in the vertebral column of Eurasian small‐bodied newts (Salamandridae: Lissotriton). in Journal of Experimental Zoology Part B - Molecular and Developmental Evolution. 2023;2023.
doi:10.1002/jez.b.23205 .

Urošević, Aleksandar, Ajduković, Maja, Vučić, Tijana, Scholtes, Stefan, Arntzen, Jan, W, Ivanović, Ana, "Regionalization and morphological integration in the vertebral column of Eurasian small‐bodied newts (Salamandridae: Lissotriton)" in Journal of Experimental Zoology Part B - Molecular and Developmental Evolution, 2023 (2023),
https://doi.org/10.1002/jez.b.23205 . .

TY  - JOUR
AU  - Ivanović, Ana
AU  - Cvijanović, Milena
AU  - Vučić, Tijana
AU  - Arntzen, Jan W.
PY  - 2023
UR  - https://link.springer.com/10.1007/s13127-022-00585-5
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5167
AB  - We examined the cranial morphology and cranial kinesis of the common toads Bufo bufo and B. spinosus with micro-computed tomography and geometric morphometrics and compared the results with published data for related species in a phylogenetic context. The species significantly diverge in skull shape. The skull of B. spinosus is shorter and higher, with a ventral arm of the squamosal bone and the jaw articulation point positioned perpendicular to the braincase, in comparison with a more lateral position in B. bufo . In either species, females have a shorter snout and a higher and wider skull at the jaw articulation point that is positioned more posteriorly, in comparison with conspecific males. High variation in the amount of bone ossification was recorded in both species, ranging from scarcely ossified and loosely connected bones to highly ossified and firmly connected bones. We also found that skull shape and inferred kinetic properties of the skull are highly variable across the Bufonini tribe. However, sample sizes are mostly small and intraspecific variation is high, which might compromise the analyses. Overall, the results suggest that developmental plasticity produces high variation in ossification and cranial kinesis, affecting individuals’ feeding performances. At the population level, this variation supports an efficient exploitation of the habitat and may promote morphological adaptation in a changing environment.
PB  - Heidelberg: Springer Heidelberg
T2  - Organisms Diversity & Evolution
T1  - Differentiation of skull morphology and cranial kinesis in common toads
VL  - 23
DO  - 10.1007/s13127-022-00585-5
SP  - 209
EP  - 219
ER  - 

@article{
author = "Ivanović, Ana and Cvijanović, Milena and Vučić, Tijana and Arntzen, Jan W.",
year = "2023",
abstract = "We examined the cranial morphology and cranial kinesis of the common toads Bufo bufo and B. spinosus with micro-computed tomography and geometric morphometrics and compared the results with published data for related species in a phylogenetic context. The species significantly diverge in skull shape. The skull of B. spinosus is shorter and higher, with a ventral arm of the squamosal bone and the jaw articulation point positioned perpendicular to the braincase, in comparison with a more lateral position in B. bufo . In either species, females have a shorter snout and a higher and wider skull at the jaw articulation point that is positioned more posteriorly, in comparison with conspecific males. High variation in the amount of bone ossification was recorded in both species, ranging from scarcely ossified and loosely connected bones to highly ossified and firmly connected bones. We also found that skull shape and inferred kinetic properties of the skull are highly variable across the Bufonini tribe. However, sample sizes are mostly small and intraspecific variation is high, which might compromise the analyses. Overall, the results suggest that developmental plasticity produces high variation in ossification and cranial kinesis, affecting individuals’ feeding performances. At the population level, this variation supports an efficient exploitation of the habitat and may promote morphological adaptation in a changing environment.",
publisher = "Heidelberg: Springer Heidelberg",
journal = "Organisms Diversity & Evolution",
title = "Differentiation of skull morphology and cranial kinesis in common toads",
volume = "23",
doi = "10.1007/s13127-022-00585-5",
pages = "209-219"
}

Ivanović, A., Cvijanović, M., Vučić, T.,& Arntzen, J. W.. (2023). Differentiation of skull morphology and cranial kinesis in common toads. in Organisms Diversity & Evolution
Heidelberg: Springer Heidelberg., 23, 209-219.
https://doi.org/10.1007/s13127-022-00585-5

Ivanović A, Cvijanović M, Vučić T, Arntzen JW. Differentiation of skull morphology and cranial kinesis in common toads. in Organisms Diversity & Evolution. 2023;23:209-219.
doi:10.1007/s13127-022-00585-5 .

Ivanović, Ana, Cvijanović, Milena, Vučić, Tijana, Arntzen, Jan W., "Differentiation of skull morphology and cranial kinesis in common toads" in Organisms Diversity & Evolution, 23 (2023):209-219,
https://doi.org/10.1007/s13127-022-00585-5 . .

TY  - JOUR
AU  - Petrović, Tamara
AU  - Vučić, Tijana
AU  - Burraco, Pablo
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Radovanović, Tijana
AU  - Šajkunić, Sanja
AU  - Ivanović, Ana
AU  - Prokić, Marko
PY  - 2023
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0306456523000153
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5463
AB  - Ectotherms are particularly sensitive to global warming due to their limited capacity to thermoregulate, which can impact their performance and fitness. From a physiological standpoint, higher temperatures often enhance biological processes that can induce the production of reactive oxygen species and result in a state of cellular oxidative stress. Temperature alters interspecific interactions, including species hybridization. Hybridization under different thermal conditions could amplify parental (genetic) incompatibilities, thus affecting a hybrid's development and distribution. Understanding the impact of global warming on the physiology of hybrids and particularly their oxidative status could help in predicting future scenarios in ecosystems and in hybrids. In the present study, we investigated the effect of water temperature on the development, growth and oxidative stress of two crested newt species and their reciprocal hybrids. Larvae of Triturus macedonicus and T. ivanbureschi, and their T. macedonicus-mothered and T. ivanbureschi-mothered hybrids were exposed for 30 days to temperatures of 19°C and 24°C. Under the higher temperature, the hybrids experienced increases in both growth and developmental rates, while parental species exhibited accelerated growth (T. macedonicus) or development (T. ivanbureschi). Warm conditions also had different effects on the oxidative status of hybrid and parental species. Parental species had enhanced antioxidant responses (catalase, glutathione peroxidase, glutathione S-transferase and SH groups), which allowed them to alleviate temperature-induced stress (revealed by the absence of oxidative damage). However, warming induced an antioxidant response in the hybrids, including oxidative damage in the form of lipid peroxidation. These findings point to a greater disruption of redox regulation and metabolic machinery in hybrid newts, which can be interpreted as the cost of hybridization that is likely linked to parental incompatibilities expressed under a higher temperature. Our study aims to improve mechanistic understanding of the resilience and distribution of hybrid species that cope with climate-driven changes.
PB  - Elsevier Ltd
T2  - Journal of Thermal Biology
T1  - Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts
VL  - 112
DO  - 10.1016/j.jtherbio.2023.103474
SP  - 103474
ER  - 

@article{
author = "Petrović, Tamara and Vučić, Tijana and Burraco, Pablo and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Radovanović, Tijana and Šajkunić, Sanja and Ivanović, Ana and Prokić, Marko",
year = "2023",
abstract = "Ectotherms are particularly sensitive to global warming due to their limited capacity to thermoregulate, which can impact their performance and fitness. From a physiological standpoint, higher temperatures often enhance biological processes that can induce the production of reactive oxygen species and result in a state of cellular oxidative stress. Temperature alters interspecific interactions, including species hybridization. Hybridization under different thermal conditions could amplify parental (genetic) incompatibilities, thus affecting a hybrid's development and distribution. Understanding the impact of global warming on the physiology of hybrids and particularly their oxidative status could help in predicting future scenarios in ecosystems and in hybrids. In the present study, we investigated the effect of water temperature on the development, growth and oxidative stress of two crested newt species and their reciprocal hybrids. Larvae of Triturus macedonicus and T. ivanbureschi, and their T. macedonicus-mothered and T. ivanbureschi-mothered hybrids were exposed for 30 days to temperatures of 19°C and 24°C. Under the higher temperature, the hybrids experienced increases in both growth and developmental rates, while parental species exhibited accelerated growth (T. macedonicus) or development (T. ivanbureschi). Warm conditions also had different effects on the oxidative status of hybrid and parental species. Parental species had enhanced antioxidant responses (catalase, glutathione peroxidase, glutathione S-transferase and SH groups), which allowed them to alleviate temperature-induced stress (revealed by the absence of oxidative damage). However, warming induced an antioxidant response in the hybrids, including oxidative damage in the form of lipid peroxidation. These findings point to a greater disruption of redox regulation and metabolic machinery in hybrid newts, which can be interpreted as the cost of hybridization that is likely linked to parental incompatibilities expressed under a higher temperature. Our study aims to improve mechanistic understanding of the resilience and distribution of hybrid species that cope with climate-driven changes.",
publisher = "Elsevier Ltd",
journal = "Journal of Thermal Biology",
title = "Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts",
volume = "112",
doi = "10.1016/j.jtherbio.2023.103474",
pages = "103474"
}

Petrović, T., Vučić, T., Burraco, P., Gavrilović, B., Despotović, S., Gavrić, J., Radovanović, T., Šajkunić, S., Ivanović, A.,& Prokić, M.. (2023). Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts. in Journal of Thermal Biology
Elsevier Ltd., 112, 103474.
https://doi.org/10.1016/j.jtherbio.2023.103474

Petrović T, Vučić T, Burraco P, Gavrilović B, Despotović S, Gavrić J, Radovanović T, Šajkunić S, Ivanović A, Prokić M. Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts. in Journal of Thermal Biology. 2023;112:103474.
doi:10.1016/j.jtherbio.2023.103474 .

Petrović, Tamara, Vučić, Tijana, Burraco, Pablo, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Radovanović, Tijana, Šajkunić, Sanja, Ivanović, Ana, Prokić, Marko, "Higher temperature induces oxidative stress in hybrids but not in parental species: A case study of crested newts" in Journal of Thermal Biology, 112 (2023):103474,
https://doi.org/10.1016/j.jtherbio.2023.103474 . .

TY  - JOUR
AU  - Bugarčić, Marko
AU  - Ivanović, Ana
AU  - Cvijanović, Milena
AU  - Vučić, Tijana
PY  - 2022
UR  - https://brill.com/view/journals/amre/aop/article-10.1163-15685381-bja10082/article-10.1163-15685381-bja10082.xml
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4873
AB  - The Macedonian crested newt T. macedonicus forms a complex hybrid zone with the Balkan crested newt T. ivanbureschi in the central part of the Balkan Peninsula. In this study, we compared life history parameters (sex ratio, survival rate and age of sexual maturation) and growth parameters (body length and mass) of T. macedonicus and T. macedonicus × T. ivanbureschi hybrids over the first three postmetamorphic years. Survival rates were high and similar for both genotypes. Sexual dimorphism in body size (length and mass) was evident at the beginning of the third postmetamorphic year, after the first breeding year, for both T. macedonicus and hybrids. We did not find clear and consistent difference in any of the analysed traits suggesting that hybridization with T. ivabureschi does not affect postmetamorphic growth and survival of T. macedonicus .
PB  - Brill Academic Publishers
T2  - Amphibia-Reptilia
T1  - Consequences of hybridization on life history and growth in postmetamorphic Triturus macedonicus
IS  - 5
VL  - 10
DO  - 10.1163/15685381-bja10082
SP  - 1
EP  - 7
ER  - 

@article{
author = "Bugarčić, Marko and Ivanović, Ana and Cvijanović, Milena and Vučić, Tijana",
year = "2022",
abstract = "The Macedonian crested newt T. macedonicus forms a complex hybrid zone with the Balkan crested newt T. ivanbureschi in the central part of the Balkan Peninsula. In this study, we compared life history parameters (sex ratio, survival rate and age of sexual maturation) and growth parameters (body length and mass) of T. macedonicus and T. macedonicus × T. ivanbureschi hybrids over the first three postmetamorphic years. Survival rates were high and similar for both genotypes. Sexual dimorphism in body size (length and mass) was evident at the beginning of the third postmetamorphic year, after the first breeding year, for both T. macedonicus and hybrids. We did not find clear and consistent difference in any of the analysed traits suggesting that hybridization with T. ivabureschi does not affect postmetamorphic growth and survival of T. macedonicus .",
publisher = "Brill Academic Publishers",
journal = "Amphibia-Reptilia",
title = "Consequences of hybridization on life history and growth in postmetamorphic Triturus macedonicus",
number = "5",
volume = "10",
doi = "10.1163/15685381-bja10082",
pages = "1-7"
}

Bugarčić, M., Ivanović, A., Cvijanović, M.,& Vučić, T.. (2022). Consequences of hybridization on life history and growth in postmetamorphic Triturus macedonicus. in Amphibia-Reptilia
Brill Academic Publishers., 10(5), 1-7.
https://doi.org/10.1163/15685381-bja10082

Bugarčić M, Ivanović A, Cvijanović M, Vučić T. Consequences of hybridization on life history and growth in postmetamorphic Triturus macedonicus. in Amphibia-Reptilia. 2022;10(5):1-7.
doi:10.1163/15685381-bja10082 .

Bugarčić, Marko, Ivanović, Ana, Cvijanović, Milena, Vučić, Tijana, "Consequences of hybridization on life history and growth in postmetamorphic Triturus macedonicus" in Amphibia-Reptilia, 10, no. 5 (2022):1-7,
https://doi.org/10.1163/15685381-bja10082 . .

TY  - JOUR
AU  - Tomović, Ljiljana
AU  - Vučić, Tijana
AU  - Anđelković, Marko
AU  - Urošević, Aleksandar
AU  - Bjelica, Vukašin
AU  - Maričić, Marko
AU  - Lakušić, Margareta
AU  - Danon, Gorana
AU  - Ivanović, Ana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5548
AB  - We present the updated distribution of batracho- and herpetofauna in southern
and south-eastern Serbia, based on literature and new field records for 16
amphibian and 22 reptile species. In these two biogeographic regions, already
recognized as biodiversity hotspots, we discovered two new amphibian species
with limited distribution: Hyla orientalis and Pelobates balcanicus. Also, one
species (Triturus ivanbureshi) has restricted distribution, while one (Bombina
bombina) is rare. Four reptile species have limited distributions and six are rare in
these regions. The presence of hybrid zones and the influence of the Mediterranean
climate make southern and south-eastern Serbia areas of great importance and the
target for future ecological and conservation studies in Serbia.
AB  - У овом раду дато је ажурирано и систематизовано распрострањење
батрахо и херепетофауне јужне и југоисточне Србије, на основу
публикованих литературних, као и нових теренских података за 16
врста водоземаца и 22 врсте гмизаваца. У ова два биогеографска
региона, претходно препознатих као подручја високог специјског
диверзитета, забележене су две нове врсте водоземаца са ограниченим
распрострањењем: Hyla orientalis и Pelobates balcanicus. Такође, једна
врста репатих водоземца (Triturus ivanbureshi) има ограничено распрострањење, док је Bombina bombina веома ретка. Четири врсте
гмизаваца су са ограниченом дистрибуцијом, а још шест су веома
ретке у ова два биогеографска региона.
Од укупно 38 врста водоземаца и гмизаваца потврђених у регионима јужне и југоисточне Србије, шест је рањиво/готово угрожено према
критеријумима IUCN-а, осам су на анексу II Директиве о стаништима,
а две су на апендиксу II CITES-а. На националном нивоу, 10 врста се
сматра критично угроженим, угроженим или рањивим по критеријумима IUCN-а, три врсте су заштићене, а 29 строго заштићене законом.
Као резултат ових истраживања, две локације у јужној Србији:
Левосоје (Бујановац) и Славујевац (Прешево), предложене су за
заштиту у окивиру успостављања националних еколошких мрежа у
Србији.
Присуство хибридних зона и утицаја Медитеранске климе чине
регионе јужне и југоисточне Србије подручјима од великог значаја за
диверзитет наше земље, и фокусом за будуће еколошке и конзервационе студије.
PB  - Belgrade: Natural History Museum
T2  - Bulletin of the Natural History Museum
T1  - Contribution to knowledge of batracho- and herpetofauna of southern and south-eastern Serbia
T1  - Прилог познавању батрахо- и херпетофауне југоисточне и јужне Србије
VL  - 15
DO  - 10.5937/bnhmb2215171T
SP  - 171
EP  - 189
ER  - 

@article{
author = "Tomović, Ljiljana and Vučić, Tijana and Anđelković, Marko and Urošević, Aleksandar and Bjelica, Vukašin and Maričić, Marko and Lakušić, Margareta and Danon, Gorana and Ivanović, Ana",
year = "2022",
abstract = "We present the updated distribution of batracho- and herpetofauna in southern
and south-eastern Serbia, based on literature and new field records for 16
amphibian and 22 reptile species. In these two biogeographic regions, already
recognized as biodiversity hotspots, we discovered two new amphibian species
with limited distribution: Hyla orientalis and Pelobates balcanicus. Also, one
species (Triturus ivanbureshi) has restricted distribution, while one (Bombina
bombina) is rare. Four reptile species have limited distributions and six are rare in
these regions. The presence of hybrid zones and the influence of the Mediterranean
climate make southern and south-eastern Serbia areas of great importance and the
target for future ecological and conservation studies in Serbia., У овом раду дато је ажурирано и систематизовано распрострањење
батрахо и херепетофауне јужне и југоисточне Србије, на основу
публикованих литературних, као и нових теренских података за 16
врста водоземаца и 22 врсте гмизаваца. У ова два биогеографска
региона, претходно препознатих као подручја високог специјског
диверзитета, забележене су две нове врсте водоземаца са ограниченим
распрострањењем: Hyla orientalis и Pelobates balcanicus. Такође, једна
врста репатих водоземца (Triturus ivanbureshi) има ограничено распрострањење, док је Bombina bombina веома ретка. Четири врсте
гмизаваца су са ограниченом дистрибуцијом, а још шест су веома
ретке у ова два биогеографска региона.
Од укупно 38 врста водоземаца и гмизаваца потврђених у регионима јужне и југоисточне Србије, шест је рањиво/готово угрожено према
критеријумима IUCN-а, осам су на анексу II Директиве о стаништима,
а две су на апендиксу II CITES-а. На националном нивоу, 10 врста се
сматра критично угроженим, угроженим или рањивим по критеријумима IUCN-а, три врсте су заштићене, а 29 строго заштићене законом.
Као резултат ових истраживања, две локације у јужној Србији:
Левосоје (Бујановац) и Славујевац (Прешево), предложене су за
заштиту у окивиру успостављања националних еколошких мрежа у
Србији.
Присуство хибридних зона и утицаја Медитеранске климе чине
регионе јужне и југоисточне Србије подручјима од великог значаја за
диверзитет наше земље, и фокусом за будуће еколошке и конзервационе студије.",
publisher = "Belgrade: Natural History Museum",
journal = "Bulletin of the Natural History Museum",
title = "Contribution to knowledge of batracho- and herpetofauna of southern and south-eastern Serbia, Прилог познавању батрахо- и херпетофауне југоисточне и јужне Србије",
volume = "15",
doi = "10.5937/bnhmb2215171T",
pages = "171-189"
}

Tomović, L., Vučić, T., Anđelković, M., Urošević, A., Bjelica, V., Maričić, M., Lakušić, M., Danon, G.,& Ivanović, A.. (2022). Contribution to knowledge of batracho- and herpetofauna of southern and south-eastern Serbia. in Bulletin of the Natural History Museum
Belgrade: Natural History Museum., 15, 171-189.
https://doi.org/10.5937/bnhmb2215171T

Tomović L, Vučić T, Anđelković M, Urošević A, Bjelica V, Maričić M, Lakušić M, Danon G, Ivanović A. Contribution to knowledge of batracho- and herpetofauna of southern and south-eastern Serbia. in Bulletin of the Natural History Museum. 2022;15:171-189.
doi:10.5937/bnhmb2215171T .

Tomović, Ljiljana, Vučić, Tijana, Anđelković, Marko, Urošević, Aleksandar, Bjelica, Vukašin, Maričić, Marko, Lakušić, Margareta, Danon, Gorana, Ivanović, Ana, "Contribution to knowledge of batracho- and herpetofauna of southern and south-eastern Serbia" in Bulletin of the Natural History Museum, 15 (2022):171-189,
https://doi.org/10.5937/bnhmb2215171T . .

TY  - CONF
AU  - Tomović, Ljiljana
AU  - Vučić, Tijana
AU  - Anđelković, Marko
AU  - Bjelica, Vukašin
AU  - Maričić, Marko
AU  - Lakušić, Margareta
AU  - Danon, Gorana
AU  - Urošević, Aleksandar
AU  - Ivanović, Ana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5272
AB  - The establishment of ecological networks (including Natura 2000) is one of the priorities in the conservation of important species and habitats in the Republic of Serbia. During the last three years (2020-2022), we conducted field research on amphibian and reptile species listed in the Annexes II & IV of the Habitat Directive. The main goals were to obtain data about precise geographic distribution, estimate population statuses and conservation threats, as well as to propose conservation measures for the studied populations and their habitats. Field studies lasted more than 70 days and included over 60 locations across the country. Focal groups were species listed in Annex II: Triturus cristatus, T. dobrogicus, T. ivanbureschi, T. macedonicus, Testudo graeca, T. hermanni, Emys orbicularis, Elaphe quatuorlineata and Vipera ursinii. Results of these studies provided a more precise geographic distribution of crested newts and their hybrid zones in Serbia. For two species, we proposed the protection of two locations at the national level: one in eastern Serbia (for T. ivanbureschi) and one in southern Serbia (for T. macedonicus). The most threatened regions, where massive habitat losses and changes were detected, are the valleys of Velika and Zapadna Morava rivers, as well as wetlands along the Danube and Sava rivers. Populations of crested newts in Serbia are endangered by several threatening factors: habitat destructions/alterations, agriculture, water pollution and climatic changes. For reptiles, the most important locations for conservation of species listed in Annex II were detected in southeastern, southern and southwestern Serbia. For three reptile species with limited distribution and small populations, we proposed two locations in southern and southwestern Serbia for protection at the national level. The main threats for those species are habitat destruction/alternation and climatic changes. Results of these studies pointed out inconsistencies in conservation priorities between the national and European levels.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Establishment of ecological networks in Serbia – field research of batracho- and herpetofauna
SP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5272
ER  - 

@conference{
author = "Tomović, Ljiljana and Vučić, Tijana and Anđelković, Marko and Bjelica, Vukašin and Maričić, Marko and Lakušić, Margareta and Danon, Gorana and Urošević, Aleksandar and Ivanović, Ana",
year = "2022",
abstract = "The establishment of ecological networks (including Natura 2000) is one of the priorities in the conservation of important species and habitats in the Republic of Serbia. During the last three years (2020-2022), we conducted field research on amphibian and reptile species listed in the Annexes II & IV of the Habitat Directive. The main goals were to obtain data about precise geographic distribution, estimate population statuses and conservation threats, as well as to propose conservation measures for the studied populations and their habitats. Field studies lasted more than 70 days and included over 60 locations across the country. Focal groups were species listed in Annex II: Triturus cristatus, T. dobrogicus, T. ivanbureschi, T. macedonicus, Testudo graeca, T. hermanni, Emys orbicularis, Elaphe quatuorlineata and Vipera ursinii. Results of these studies provided a more precise geographic distribution of crested newts and their hybrid zones in Serbia. For two species, we proposed the protection of two locations at the national level: one in eastern Serbia (for T. ivanbureschi) and one in southern Serbia (for T. macedonicus). The most threatened regions, where massive habitat losses and changes were detected, are the valleys of Velika and Zapadna Morava rivers, as well as wetlands along the Danube and Sava rivers. Populations of crested newts in Serbia are endangered by several threatening factors: habitat destructions/alterations, agriculture, water pollution and climatic changes. For reptiles, the most important locations for conservation of species listed in Annex II were detected in southeastern, southern and southwestern Serbia. For three reptile species with limited distribution and small populations, we proposed two locations in southern and southwestern Serbia for protection at the national level. The main threats for those species are habitat destruction/alternation and climatic changes. Results of these studies pointed out inconsistencies in conservation priorities between the national and European levels.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Establishment of ecological networks in Serbia – field research of batracho- and herpetofauna",
pages = "71",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5272"
}

Tomović, L., Vučić, T., Anđelković, M., Bjelica, V., Maričić, M., Lakušić, M., Danon, G., Urošević, A.,& Ivanović, A.. (2022). Establishment of ecological networks in Serbia – field research of batracho- and herpetofauna. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 71.
https://hdl.handle.net/21.15107/rcub_ibiss_5272

Tomović L, Vučić T, Anđelković M, Bjelica V, Maričić M, Lakušić M, Danon G, Urošević A, Ivanović A. Establishment of ecological networks in Serbia – field research of batracho- and herpetofauna. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:71.
https://hdl.handle.net/21.15107/rcub_ibiss_5272 .

Tomović, Ljiljana, Vučić, Tijana, Anđelković, Marko, Bjelica, Vukašin, Maričić, Marko, Lakušić, Margareta, Danon, Gorana, Urošević, Aleksandar, Ivanović, Ana, "Establishment of ecological networks in Serbia – field research of batracho- and herpetofauna" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):71,
https://hdl.handle.net/21.15107/rcub_ibiss_5272 .

TY  - CONF
AU  - Urošević, Aleksandar
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Scholtes, Stefan
AU  - Arntzen, Jan
AU  - Ivanović, Ana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5231
AB  - Serially homologous structures, such as the vertebral column, often undergo 
functional and evolutionary diversification and are a good model-system for studies of 
regionalization and morphological integration. We studied these topics in the vertebral 
column (atlas, trunk and sacral vertebrae) of the closely related taxa of small-bodied 
newts – Lissotriton schmidtleri, L. vulgaris ampelensis, L. v. meridionalis and L. v. 
vulgaris, using 3D geometric morphometrics on models that were acquired with micro CT scanning. Two different statistical approaches were employed to test for vertebral 
regionalization and overall morphological integration, namely segmented linear 
regression (SLR) and a partial least squares method (PLS) We observed a common 
pattern of regionalization, with a transition point after the 5th trunk vertebra. It 
corresponds with the antero-posterior transition common for tetrapods. 
Morphological integration, accessed via PLS analysis, is strongest at the 6th and 7th
trunk vertebrae, while the anterior and distal parts of the vertebral column are less 
integrated. The PLS analysis of the asymmetric component of shape variation revealed 
a weak integration, statistically significant only among subsequent trunk vertebrae. In 
summary, the vertebral column of the closely related Lissotriton newts is subtly 
regionalized, while being morphologically integrated overall. There is a complex 
relationship between regionalization and morphological integration of the vertebral 
column, most likely influenced by the newt’s bi-phasic life cycle that instigates different 
functional constraints in the aquatic and terrestrial life stages
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Vertebral regionalization vs. morphological integration in Lissotriton newts
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5231
ER  - 

@conference{
author = "Urošević, Aleksandar and Ajduković, Maja and Vučić, Tijana and Scholtes, Stefan and Arntzen, Jan and Ivanović, Ana",
year = "2022",
abstract = "Serially homologous structures, such as the vertebral column, often undergo 
functional and evolutionary diversification and are a good model-system for studies of 
regionalization and morphological integration. We studied these topics in the vertebral 
column (atlas, trunk and sacral vertebrae) of the closely related taxa of small-bodied 
newts – Lissotriton schmidtleri, L. vulgaris ampelensis, L. v. meridionalis and L. v. 
vulgaris, using 3D geometric morphometrics on models that were acquired with micro CT scanning. Two different statistical approaches were employed to test for vertebral 
regionalization and overall morphological integration, namely segmented linear 
regression (SLR) and a partial least squares method (PLS) We observed a common 
pattern of regionalization, with a transition point after the 5th trunk vertebra. It 
corresponds with the antero-posterior transition common for tetrapods. 
Morphological integration, accessed via PLS analysis, is strongest at the 6th and 7th
trunk vertebrae, while the anterior and distal parts of the vertebral column are less 
integrated. The PLS analysis of the asymmetric component of shape variation revealed 
a weak integration, statistically significant only among subsequent trunk vertebrae. In 
summary, the vertebral column of the closely related Lissotriton newts is subtly 
regionalized, while being morphologically integrated overall. There is a complex 
relationship between regionalization and morphological integration of the vertebral 
column, most likely influenced by the newt’s bi-phasic life cycle that instigates different 
functional constraints in the aquatic and terrestrial life stages",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Vertebral regionalization vs. morphological integration in Lissotriton newts",
pages = "38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5231"
}

Urošević, A., Ajduković, M., Vučić, T., Scholtes, S., Arntzen, J.,& Ivanović, A.. (2022). Vertebral regionalization vs. morphological integration in Lissotriton newts. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 38.
https://hdl.handle.net/21.15107/rcub_ibiss_5231

Urošević A, Ajduković M, Vučić T, Scholtes S, Arntzen J, Ivanović A. Vertebral regionalization vs. morphological integration in Lissotriton newts. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:38.
https://hdl.handle.net/21.15107/rcub_ibiss_5231 .

Urošević, Aleksandar, Ajduković, Maja, Vučić, Tijana, Scholtes, Stefan, Arntzen, Jan, Ivanović, Ana, "Vertebral regionalization vs. morphological integration in Lissotriton newts" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):38,
https://hdl.handle.net/21.15107/rcub_ibiss_5231 .

TY  - CONF
AU  - Bugarčić, Marko
AU  - Ivanović, Ana
AU  - Cvijanović, Milena
AU  - Ajduković, Maja
AU  - Wielstra, Ben
AU  - Vučić, Tijana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5228
AB  - In developmental biology a plethora of studies support the existence of a
conserved stage during the ontogeny of vertebrates – the pharyngula. The
developmental hourglass model predicts that the most conserved morphological
pattern occurs in the middle of embryonic development. We explored external
morphology of the craniofacial region of Triturus newts using 3D geometric
morphometrics. The craniofacial region was selected because it experiences
pleiotropic developmental constrains due to its major role in feeding, respiration and
the housing of substantial parts of nervous and sensory systems. We aimed to uncover
differences in variance between successive developmental stages, because it is
proposed that the more constrained stages should have the lowest variance. In
addition, we explored differences in the craniofacial shape between successive
developmental stages. Stages included start from the moment after the completion of
neurulation and span the mid-tailbud phase. The least amount of variance in shape was
recorded at stage 24 (according to D’Amen and colleagues). In general, this stage is
characterized by changes in overall head shape, the distinction of optical vesicles,
intensive somitogenesis and formation of the tailbud which overgrows the blastopore,
indicating that these processes could be highly constrained. Only significant
craniofacial shape change occurred between this and successive stage. The most
pronounced differences were due to growing of the head region and gill bud balancers.
Optic vesicles became concave due to inductive processes preceding eye formation.
These preliminary results indicate that stage 24 could be the most conserved one
during early ontogeny as it is preceded and followed by stages exhibiting more
variance in shape. Our results indicate that the craniofacial development represents a
suitable model system for testing the hourglass model of development. Larger sample
size, exploration of the entire embryonic development and ontogeny of additional newt
species is needed to confirm our preliminary findings.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model
SP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5228
ER  - 

@conference{
author = "Bugarčić, Marko and Ivanović, Ana and Cvijanović, Milena and Ajduković, Maja and Wielstra, Ben and Vučić, Tijana",
year = "2022",
abstract = "In developmental biology a plethora of studies support the existence of a
conserved stage during the ontogeny of vertebrates – the pharyngula. The
developmental hourglass model predicts that the most conserved morphological
pattern occurs in the middle of embryonic development. We explored external
morphology of the craniofacial region of Triturus newts using 3D geometric
morphometrics. The craniofacial region was selected because it experiences
pleiotropic developmental constrains due to its major role in feeding, respiration and
the housing of substantial parts of nervous and sensory systems. We aimed to uncover
differences in variance between successive developmental stages, because it is
proposed that the more constrained stages should have the lowest variance. In
addition, we explored differences in the craniofacial shape between successive
developmental stages. Stages included start from the moment after the completion of
neurulation and span the mid-tailbud phase. The least amount of variance in shape was
recorded at stage 24 (according to D’Amen and colleagues). In general, this stage is
characterized by changes in overall head shape, the distinction of optical vesicles,
intensive somitogenesis and formation of the tailbud which overgrows the blastopore,
indicating that these processes could be highly constrained. Only significant
craniofacial shape change occurred between this and successive stage. The most
pronounced differences were due to growing of the head region and gill bud balancers.
Optic vesicles became concave due to inductive processes preceding eye formation.
These preliminary results indicate that stage 24 could be the most conserved one
during early ontogeny as it is preceded and followed by stages exhibiting more
variance in shape. Our results indicate that the craniofacial development represents a
suitable model system for testing the hourglass model of development. Larger sample
size, exploration of the entire embryonic development and ontogeny of additional newt
species is needed to confirm our preliminary findings.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model",
pages = "42",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5228"
}

Bugarčić, M., Ivanović, A., Cvijanović, M., Ajduković, M., Wielstra, B.,& Vučić, T.. (2022). Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 42.
https://hdl.handle.net/21.15107/rcub_ibiss_5228

Bugarčić M, Ivanović A, Cvijanović M, Ajduković M, Wielstra B, Vučić T. Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:42.
https://hdl.handle.net/21.15107/rcub_ibiss_5228 .

Bugarčić, Marko, Ivanović, Ana, Cvijanović, Milena, Ajduković, Maja, Wielstra, Ben, Vučić, Tijana, "Craniofacial development of Triturus newts – a suitable model system for  testing the developmental hourglass model" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):42,
https://hdl.handle.net/21.15107/rcub_ibiss_5228 .

TY  - CONF
AU  - Cvijanović, Milena
AU  - Ajduković, Maja
AU  - Ivanović, Ana
AU  - Arntzen, Jan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5134
AB  - In the skin of many terrestrial anuran taxa, a specific acellular mineralized tissue
layer (the Eberth-Katschenko layer) has been documented. This layer is generally
positioned in between the stratum spongiosum and the stratum compactum of the
dermis and has a role in reducing water loss. Here, we document and compare the
amount of calcium deposition in the skin of the head and the parotoids (the external
skin glands) in males and females of the common toad Bufo bufo and the spined toad B.
spinosus. Bufo bufo and B. spinosus are morphologically similar and both show a
conspicuous sexual dimorphism. Using standard histological techniques, we detected
calcium as an amorphous ‘ground substance’ located in the stratum spongiosum, just
above the Eberth-Katschenko layer. We observed large variability in the number of
calcium deposits between the species and the sexes. Using micro-computed
tomography (micro-CT) we were able to quantify the level of the toad skin calcification.
Bufo spinosus females stand out compared to conspecific males and B. bufo on account
of a strong calcification of the dorsal and ventral skin and the parotoid glands. Species
and sexes significantly differed in the amount of calcium deposits (Fisher’s exact test,
p < 0.001) and pairwise comparisons showed that groups differed from one another (p
< 0.05), suggesting size and sexual dimorphism in these traits. We conclude that microCT scanning is useful for the quantification of calcified structures in the anuran skin,
and keeps a promise for further studies on taxonomic and geographic variation.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - What is hiding in the Bufo skin? Revealing of the structures in the skin of European toads using standard histological and micro-CT techniques
SP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5134
ER  - 

@conference{
author = "Cvijanović, Milena and Ajduković, Maja and Ivanović, Ana and Arntzen, Jan",
year = "2022",
abstract = "In the skin of many terrestrial anuran taxa, a specific acellular mineralized tissue
layer (the Eberth-Katschenko layer) has been documented. This layer is generally
positioned in between the stratum spongiosum and the stratum compactum of the
dermis and has a role in reducing water loss. Here, we document and compare the
amount of calcium deposition in the skin of the head and the parotoids (the external
skin glands) in males and females of the common toad Bufo bufo and the spined toad B.
spinosus. Bufo bufo and B. spinosus are morphologically similar and both show a
conspicuous sexual dimorphism. Using standard histological techniques, we detected
calcium as an amorphous ‘ground substance’ located in the stratum spongiosum, just
above the Eberth-Katschenko layer. We observed large variability in the number of
calcium deposits between the species and the sexes. Using micro-computed
tomography (micro-CT) we were able to quantify the level of the toad skin calcification.
Bufo spinosus females stand out compared to conspecific males and B. bufo on account
of a strong calcification of the dorsal and ventral skin and the parotoid glands. Species
and sexes significantly differed in the amount of calcium deposits (Fisher’s exact test,
p < 0.001) and pairwise comparisons showed that groups differed from one another (p
< 0.05), suggesting size and sexual dimorphism in these traits. We conclude that microCT scanning is useful for the quantification of calcified structures in the anuran skin,
and keeps a promise for further studies on taxonomic and geographic variation.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "What is hiding in the Bufo skin? Revealing of the structures in the skin of European toads using standard histological and micro-CT techniques",
pages = "32",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5134"
}

Cvijanović, M., Ajduković, M., Ivanović, A.,& Arntzen, J.. (2022). What is hiding in the Bufo skin? Revealing of the structures in the skin of European toads using standard histological and micro-CT techniques. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 32.
https://hdl.handle.net/21.15107/rcub_ibiss_5134

Cvijanović M, Ajduković M, Ivanović A, Arntzen J. What is hiding in the Bufo skin? Revealing of the structures in the skin of European toads using standard histological and micro-CT techniques. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:32.
https://hdl.handle.net/21.15107/rcub_ibiss_5134 .

Cvijanović, Milena, Ajduković, Maja, Ivanović, Ana, Arntzen, Jan, "What is hiding in the Bufo skin? Revealing of the structures in the skin of European toads using standard histological and micro-CT techniques" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):32,
https://hdl.handle.net/21.15107/rcub_ibiss_5134 .

TY  - DATA
AU  - Scholtes, Stefan
AU  - Willem, Jan
AU  - Ivanović, Ana
AU  - Ajduković, Maja
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4661
AB  - Raw landmark coordinates
Each trunk vertebrae (T1 to T13) per species obtained from landmarking 3D surface models of the specimens used in this study can be found in the zip file "Raw landmark coordinates.rar". Each file is labelled as follows: Vertebrae-"Species"-"Collection label"-"Vertebrae number". For example "Vertebrae-Ampelensis-OZ-62-G22595-T1". Collection labels can be found in Appendix table S1 and individual landmark descriptions in Appendix table S2.

Appendix table S1
Collection material analyzed and type of preservation: ethanol preserved and whole-mounts glycerine stored skeletons. Museum codes are: IBISS – University of Belgrade, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, RMNH.RENA and ZMA.RENA – Naturalis Biodiversity Center, Leiden, The Netherlands. N - sample size.

Appendix table S2
Brief anatomical descriptions of 27 landmarks configured to Lissotriton trunk vertebrae.

Appendix table S3
Frequency (%) of morphotypes over Lissotriton taxon / population and vertebra number.

Appendix table S4
Results on the phenotypic trajectory analysis with P-values for pairwise statistical assessment of a) the amount of species-specific shape changes along trunk region, i.e., trajectory size, b) the general orientation of shape changes in multivariate shape space, i.e., trajectory direction and c) the pattern of shape changes of vertebrae along the trunk region, i.e., trajectory shape.

Supplementary figure S1

Geographical distribution of the (sub-)species of the genus Lissotriton after Arntzen et al., 2009, Ianella et al., 2017 and Wielstra et al.,2018. Taxa are coded by colours as shown in the legend. Numbered dots indicate the localities of the populations that were studied, as also listed in Supplementary table S1. Note that no material was available for L. italicus and L. lantzi.


Supplementary figure S2

Phenotypic trajectories of shape changes along the trunk region of the newt genus Lissotriton expressed over the first (PC1) and third principal component axis (PC3). Trajectories were calculated for nine (sub-)species. The three populations of the L. helveticus were analysed separately. Taxa are coded by colours as shown in the legend. Connected dots represent the mean vertebrae (T) shape from T1 to T12, as is highlighted for L. helveticus from Marcillé la Ville, France. Three-dimensional surface models visualize the corresponding shape changes.


Supplementary figure S2_3D.

Three-dimensional graph showing phenotypic trajectories of shape changes along the trunk region in nine (sub-)species of the newt genus Lissotriton. Open the file with a web browser such as Google Chrome or Mozilla Firefox to visualize the trajectories in three dimensional morphospace that as defined by the first, second and third principal component axis (PC1, PC2 and PC3).
PB  - Hoboken: Wiley
T2  - Journal of Anatomy
T1  - Supplementary material "Variation in vertebrae shape across small-bodied newts reveals functional and developmental constraints acting upon the trunk region"
DO  - 10.6084/m9.figshare.14254406
ER  - 

@misc{
author = "Scholtes, Stefan and Willem, Jan and Ivanović, Ana and Ajduković, Maja",
year = "2021",
abstract = "Raw landmark coordinates
Each trunk vertebrae (T1 to T13) per species obtained from landmarking 3D surface models of the specimens used in this study can be found in the zip file "Raw landmark coordinates.rar". Each file is labelled as follows: Vertebrae-"Species"-"Collection label"-"Vertebrae number". For example "Vertebrae-Ampelensis-OZ-62-G22595-T1". Collection labels can be found in Appendix table S1 and individual landmark descriptions in Appendix table S2.

Appendix table S1
Collection material analyzed and type of preservation: ethanol preserved and whole-mounts glycerine stored skeletons. Museum codes are: IBISS – University of Belgrade, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, RMNH.RENA and ZMA.RENA – Naturalis Biodiversity Center, Leiden, The Netherlands. N - sample size.

Appendix table S2
Brief anatomical descriptions of 27 landmarks configured to Lissotriton trunk vertebrae.

Appendix table S3
Frequency (%) of morphotypes over Lissotriton taxon / population and vertebra number.

Appendix table S4
Results on the phenotypic trajectory analysis with P-values for pairwise statistical assessment of a) the amount of species-specific shape changes along trunk region, i.e., trajectory size, b) the general orientation of shape changes in multivariate shape space, i.e., trajectory direction and c) the pattern of shape changes of vertebrae along the trunk region, i.e., trajectory shape.

Supplementary figure S1

Geographical distribution of the (sub-)species of the genus Lissotriton after Arntzen et al., 2009, Ianella et al., 2017 and Wielstra et al.,2018. Taxa are coded by colours as shown in the legend. Numbered dots indicate the localities of the populations that were studied, as also listed in Supplementary table S1. Note that no material was available for L. italicus and L. lantzi.


Supplementary figure S2

Phenotypic trajectories of shape changes along the trunk region of the newt genus Lissotriton expressed over the first (PC1) and third principal component axis (PC3). Trajectories were calculated for nine (sub-)species. The three populations of the L. helveticus were analysed separately. Taxa are coded by colours as shown in the legend. Connected dots represent the mean vertebrae (T) shape from T1 to T12, as is highlighted for L. helveticus from Marcillé la Ville, France. Three-dimensional surface models visualize the corresponding shape changes.


Supplementary figure S2_3D.

Three-dimensional graph showing phenotypic trajectories of shape changes along the trunk region in nine (sub-)species of the newt genus Lissotriton. Open the file with a web browser such as Google Chrome or Mozilla Firefox to visualize the trajectories in three dimensional morphospace that as defined by the first, second and third principal component axis (PC1, PC2 and PC3).",
publisher = "Hoboken: Wiley",
journal = "Journal of Anatomy",
title = "Supplementary material "Variation in vertebrae shape across small-bodied newts reveals functional and developmental constraints acting upon the trunk region"",
doi = "10.6084/m9.figshare.14254406"
}

Scholtes, S., Willem, J., Ivanović, A.,& Ajduković, M.. (2021). Supplementary material "Variation in vertebrae shape across small-bodied newts reveals functional and developmental constraints acting upon the trunk region". in Journal of Anatomy
Hoboken: Wiley..
https://doi.org/10.6084/m9.figshare.14254406

Scholtes S, Willem J, Ivanović A, Ajduković M. Supplementary material "Variation in vertebrae shape across small-bodied newts reveals functional and developmental constraints acting upon the trunk region". in Journal of Anatomy. 2021;.
doi:10.6084/m9.figshare.14254406 .

Scholtes, Stefan, Willem, Jan, Ivanović, Ana, Ajduković, Maja, "Supplementary material "Variation in vertebrae shape across small-bodied newts reveals functional and developmental constraints acting upon the trunk region"" in Journal of Anatomy (2021),
https://doi.org/10.6084/m9.figshare.14254406 . .

TY  - JOUR
AU  - Urošević, Aleksandar
AU  - Ajduković, Maja
AU  - Arntzen, Jan W.
AU  - Ivanović, Ana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3617
AB  - Serial homology or the repetition of equivalent developmental units and their derivatives is a phenomenon encountered in a variety of organisms, with the vertebrate axial skeleton as one of the most notable examples. Serially homologous structures can be viewed as an appropriate model system for studying morphological integration and modularity, due to the strong impact of development on their covariation. Here, we explored the pattern of morphological integration of the cranium and the first three serially homologous structures (atlas, first, and second trunk vertebrae) in salamandrid salamanders, using micro‐CT scanning and three‐dimensional geometric morphometrics. We explored the integration between structures at static and evolutionary levels. Effects of allometry on patterns of modularity were also taken into account. At the static level (within species), we analyzed inter‐individual variation in shape to detect functional modules and intra‐individual variation to detect developmental modules. Significant integration (based on inter‐individual variation) among all structures was detected and allometry is shown to be an important integrating factor. The pattern of intra‐individual, asymmetric variation indicates statistically significant developmental integration between the cranium and the atlas and between the first two trunk vertebrae. At the evolutionary level (among species), the cranium, atlas, and trunk vertebrae separate as different modules. Our results show that morphological integration at the evolutionary level coincides with morphological and functional differentiation of the axial skeleton, allowing the more or less independent evolutionary changes of the cranial skeleton and the vertebral column, regardless of the relatively strong integration at the static level. The observed patterns of morphological integration differ across levels, indicating different impacts of developmental and phylogenetic constraints and functional demands.
PB  - Wiley
T2  - Journal of Zoological Systematics and Evolutionary Research
T1  - Morphological integration and serial homology: A case study of the cranium and anterior vertebrae in salamanders
IS  - 4
VL  - 58
DO  - 10.1111/jzs.12374
SP  - 1206
EP  - 1219
ER  - 

@article{
author = "Urošević, Aleksandar and Ajduković, Maja and Arntzen, Jan W. and Ivanović, Ana",
year = "2020",
abstract = "Serial homology or the repetition of equivalent developmental units and their derivatives is a phenomenon encountered in a variety of organisms, with the vertebrate axial skeleton as one of the most notable examples. Serially homologous structures can be viewed as an appropriate model system for studying morphological integration and modularity, due to the strong impact of development on their covariation. Here, we explored the pattern of morphological integration of the cranium and the first three serially homologous structures (atlas, first, and second trunk vertebrae) in salamandrid salamanders, using micro‐CT scanning and three‐dimensional geometric morphometrics. We explored the integration between structures at static and evolutionary levels. Effects of allometry on patterns of modularity were also taken into account. At the static level (within species), we analyzed inter‐individual variation in shape to detect functional modules and intra‐individual variation to detect developmental modules. Significant integration (based on inter‐individual variation) among all structures was detected and allometry is shown to be an important integrating factor. The pattern of intra‐individual, asymmetric variation indicates statistically significant developmental integration between the cranium and the atlas and between the first two trunk vertebrae. At the evolutionary level (among species), the cranium, atlas, and trunk vertebrae separate as different modules. Our results show that morphological integration at the evolutionary level coincides with morphological and functional differentiation of the axial skeleton, allowing the more or less independent evolutionary changes of the cranial skeleton and the vertebral column, regardless of the relatively strong integration at the static level. The observed patterns of morphological integration differ across levels, indicating different impacts of developmental and phylogenetic constraints and functional demands.",
publisher = "Wiley",
journal = "Journal of Zoological Systematics and Evolutionary Research",
title = "Morphological integration and serial homology: A case study of the cranium and anterior vertebrae in salamanders",
number = "4",
volume = "58",
doi = "10.1111/jzs.12374",
pages = "1206-1219"
}

Urošević, A., Ajduković, M., Arntzen, J. W.,& Ivanović, A.. (2020). Morphological integration and serial homology: A case study of the cranium and anterior vertebrae in salamanders. in Journal of Zoological Systematics and Evolutionary Research
Wiley., 58(4), 1206-1219.
https://doi.org/10.1111/jzs.12374

Urošević A, Ajduković M, Arntzen JW, Ivanović A. Morphological integration and serial homology: A case study of the cranium and anterior vertebrae in salamanders. in Journal of Zoological Systematics and Evolutionary Research. 2020;58(4):1206-1219.
doi:10.1111/jzs.12374 .

Urošević, Aleksandar, Ajduković, Maja, Arntzen, Jan W., Ivanović, Ana, "Morphological integration and serial homology: A case study of the cranium and anterior vertebrae in salamanders" in Journal of Zoological Systematics and Evolutionary Research, 58, no. 4 (2020):1206-1219,
https://doi.org/10.1111/jzs.12374 . .

TY  - JOUR
AU  - Vučić, Tijana
AU  - Ivanović, Ana
AU  - Nikolić, Sonja
AU  - Jovanović, Jovana
AU  - Cvijanović, Milena
PY  - 2020
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46642000026V
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4036
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/5220
AB  - During three consecutive years, we compared the reproductive characteristics and oviposition dynamics of two crested newt species, Triturus ivanbureschi and T. macedonicus. These two well-defined species are of special interest because of complex interactions at their contact zone, which include hybridization, species replacement and asymmetric mitochondrial DNA introgression. In common garden experiments, females were introduced to conspecific males, to males of other species and to hybrid males. We monitored the total number and size of the deposited eggs, as well as the dynamics and duration of oviposition during three consecutive years. The number of deposited eggs of Triturus species was much higher than previously reported. Triturus macedonicus lay more eggs that were of larger size in comparison to T. ivanbureschi. Also, the onset of T. macedonicus oviposition was considerably delayed compared to T. ivanbureschi. These are fundamental data, important for understanding complex species interactions in their contact zones.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Reproductive characteristics of two Triturus species (Amphibia: Caudata)
IS  - 3
VL  - 72
DO  - 10.2298/ABS200328026V
SP  - 321
EP  - 328
ER  - 

@article{
author = "Vučić, Tijana and Ivanović, Ana and Nikolić, Sonja and Jovanović, Jovana and Cvijanović, Milena",
year = "2020",
abstract = "During three consecutive years, we compared the reproductive characteristics and oviposition dynamics of two crested newt species, Triturus ivanbureschi and T. macedonicus. These two well-defined species are of special interest because of complex interactions at their contact zone, which include hybridization, species replacement and asymmetric mitochondrial DNA introgression. In common garden experiments, females were introduced to conspecific males, to males of other species and to hybrid males. We monitored the total number and size of the deposited eggs, as well as the dynamics and duration of oviposition during three consecutive years. The number of deposited eggs of Triturus species was much higher than previously reported. Triturus macedonicus lay more eggs that were of larger size in comparison to T. ivanbureschi. Also, the onset of T. macedonicus oviposition was considerably delayed compared to T. ivanbureschi. These are fundamental data, important for understanding complex species interactions in their contact zones.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Reproductive characteristics of two Triturus species (Amphibia: Caudata)",
number = "3",
volume = "72",
doi = "10.2298/ABS200328026V",
pages = "321-328"
}

Vučić, T., Ivanović, A., Nikolić, S., Jovanović, J.,& Cvijanović, M.. (2020). Reproductive characteristics of two Triturus species (Amphibia: Caudata). in Archives of Biological Sciences
Serbian Biological Society., 72(3), 321-328.
https://doi.org/10.2298/ABS200328026V

Vučić T, Ivanović A, Nikolić S, Jovanović J, Cvijanović M. Reproductive characteristics of two Triturus species (Amphibia: Caudata). in Archives of Biological Sciences. 2020;72(3):321-328.
doi:10.2298/ABS200328026V .

Vučić, Tijana, Ivanović, Ana, Nikolić, Sonja, Jovanović, Jovana, Cvijanović, Milena, "Reproductive characteristics of two Triturus species (Amphibia: Caudata)" in Archives of Biological Sciences, 72, no. 3 (2020):321-328,
https://doi.org/10.2298/ABS200328026V . .

TY  - JOUR
AU  - Vučić, Tijana
AU  - Sibinović, Maša
AU  - Vukov, Tanja
AU  - Tomašević Kolarov, Nataša
AU  - Cvijanović, Milena
AU  - Ivanović, Ana
PY  - 2019
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/evo.13743
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3348
AB  - In vertebrates with complex, biphasic, life cycles, larvae have a distinct morphology and ecological preferences compared to metamorphosed juveniles and adults. In amphibians, abrupt and rapid metamorphic changes transform aquatic larvae to terrestrial juveniles. The main aim of this study is to test whether, relative to larval stages, metamorphosis (1) resets the pattern of variation between ontogenetic stages and species, (2) constrains intraspecific morphological variability, and (3) similar to the "hour-glass" model reduces morphological disparity. We explore postembryonic ontogenetic trajectories of head shape (from hatching to completed metamorphosis) of two well-defined, morphologically distinct Triturus newts species and their F1 hybrids. Variation in head shape is quantified and compared on two levels: dynamic (across ontogenetic stages) and static (at a particular stage). Our results show that the ontogenetic trajectories diverge early during development and continue to diverge throughout larval stages and metamorphosis. The high within-group variance and the largest disparity level (between-group variance) characterize the metamorphosed stage. Hence, our results indicate that metamorphosis does not canalize head shape variation generated during larval development and that metamorphosed phenotype is not more constrained relative to larval ones. Therefore, metamorphosis cannot be regarded as a developmental constraint, at least not for salamander head shape.
T2  - Evolution; International Journal of Organic Evolution
T1  - Testing the evolutionary constraints of metamorphosis: The ontogeny of head shape in Triturus newts.
DO  - 10.1111/evo.13743
ER  - 

@article{
author = "Vučić, Tijana and Sibinović, Maša and Vukov, Tanja and Tomašević Kolarov, Nataša and Cvijanović, Milena and Ivanović, Ana",
year = "2019",
abstract = "In vertebrates with complex, biphasic, life cycles, larvae have a distinct morphology and ecological preferences compared to metamorphosed juveniles and adults. In amphibians, abrupt and rapid metamorphic changes transform aquatic larvae to terrestrial juveniles. The main aim of this study is to test whether, relative to larval stages, metamorphosis (1) resets the pattern of variation between ontogenetic stages and species, (2) constrains intraspecific morphological variability, and (3) similar to the "hour-glass" model reduces morphological disparity. We explore postembryonic ontogenetic trajectories of head shape (from hatching to completed metamorphosis) of two well-defined, morphologically distinct Triturus newts species and their F1 hybrids. Variation in head shape is quantified and compared on two levels: dynamic (across ontogenetic stages) and static (at a particular stage). Our results show that the ontogenetic trajectories diverge early during development and continue to diverge throughout larval stages and metamorphosis. The high within-group variance and the largest disparity level (between-group variance) characterize the metamorphosed stage. Hence, our results indicate that metamorphosis does not canalize head shape variation generated during larval development and that metamorphosed phenotype is not more constrained relative to larval ones. Therefore, metamorphosis cannot be regarded as a developmental constraint, at least not for salamander head shape.",
journal = "Evolution; International Journal of Organic Evolution",
title = "Testing the evolutionary constraints of metamorphosis: The ontogeny of head shape in Triturus newts.",
doi = "10.1111/evo.13743"
}

Vučić, T., Sibinović, M., Vukov, T., Tomašević Kolarov, N., Cvijanović, M.,& Ivanović, A.. (2019). Testing the evolutionary constraints of metamorphosis: The ontogeny of head shape in Triturus newts.. in Evolution; International Journal of Organic Evolution.
https://doi.org/10.1111/evo.13743

Vučić T, Sibinović M, Vukov T, Tomašević Kolarov N, Cvijanović M, Ivanović A. Testing the evolutionary constraints of metamorphosis: The ontogeny of head shape in Triturus newts.. in Evolution; International Journal of Organic Evolution. 2019;.
doi:10.1111/evo.13743 .

Vučić, Tijana, Sibinović, Maša, Vukov, Tanja, Tomašević Kolarov, Nataša, Cvijanović, Milena, Ivanović, Ana, "Testing the evolutionary constraints of metamorphosis: The ontogeny of head shape in Triturus newts." in Evolution; International Journal of Organic Evolution (2019),
https://doi.org/10.1111/evo.13743 . .

TY  - CONF
AU  - Vučić, Tijana
AU  - Nikolić, Sonja
AU  - Jovanović, Jovana
AU  - Ivanović, Ana
AU  - Cvijanović, Milena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5589
AB  - We explored some of the life history traits of Triturus ivanbureschi and T. macedonicus. These two species
hybridize and form a large introgressive zone in central Balkan Peninsula which follows the specific species
displacement scenario: Triturus macedonicus widens its range and transects the range of T. ivanbureschi. One of
the important questions is whether, in the hybrid zone, one species has advantage over the other in reproduction
or survival. During three consecutive years, we compared reproductive potentials of the two species in common
garden experiments. The females of both species were introduced both to conspecific males and males of the other
species. We monitored their reproductive success: proportions of egg-laying females, the total number of deposited
eggs, and dynamics and duration of oviposition. Our results indicate that fecundity is largely influenced by
environmental factors as well as females’ affinities towards males, but specific differences in reproductive
potential between the species were not found.
PB  - Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca
C3  - XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy
T1  - Reproductive potentials of two Triturus species from their hybrid zone
SP  - 303
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5589
ER  - 

@conference{
author = "Vučić, Tijana and Nikolić, Sonja and Jovanović, Jovana and Ivanović, Ana and Cvijanović, Milena",
year = "2019",
abstract = "We explored some of the life history traits of Triturus ivanbureschi and T. macedonicus. These two species
hybridize and form a large introgressive zone in central Balkan Peninsula which follows the specific species
displacement scenario: Triturus macedonicus widens its range and transects the range of T. ivanbureschi. One of
the important questions is whether, in the hybrid zone, one species has advantage over the other in reproduction
or survival. During three consecutive years, we compared reproductive potentials of the two species in common
garden experiments. The females of both species were introduced both to conspecific males and males of the other
species. We monitored their reproductive success: proportions of egg-laying females, the total number of deposited
eggs, and dynamics and duration of oviposition. Our results indicate that fecundity is largely influenced by
environmental factors as well as females’ affinities towards males, but specific differences in reproductive
potential between the species were not found.",
publisher = "Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca",
journal = "XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy",
title = "Reproductive potentials of two Triturus species from their hybrid zone",
pages = "303",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5589"
}

Vučić, T., Nikolić, S., Jovanović, J., Ivanović, A.,& Cvijanović, M.. (2019). Reproductive potentials of two Triturus species from their hybrid zone. in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy
Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca., 303.
https://hdl.handle.net/21.15107/rcub_ibiss_5589

Vučić T, Nikolić S, Jovanović J, Ivanović A, Cvijanović M. Reproductive potentials of two Triturus species from their hybrid zone. in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy. 2019;:303.
https://hdl.handle.net/21.15107/rcub_ibiss_5589 .

Vučić, Tijana, Nikolić, Sonja, Jovanović, Jovana, Ivanović, Ana, Cvijanović, Milena, "Reproductive potentials of two Triturus species from their hybrid zone" in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy (2019):303,
https://hdl.handle.net/21.15107/rcub_ibiss_5589 .

TY  - CONF
AU  - Urošević, Aleksandar
AU  - Ajduković, Maja
AU  - Arntzen, Jan W
AU  - Ivanović, Ana
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5527
AB  - Using micro-CT scanning and 3D geometric morphometrics of newt craniums and axial skeletons (first
three vertebrae) we explored the pattern of morphological integration. We tested if i) directly connected serially
homologous structures are more integrated than separated ones and ii) morphological integration coincides with
regional differentiation. We applied a multilevel approach by analyzing patterns of integration at static and
evolutionary levels, i.e., within and between species respectively. At the static level we choose the genus Triturus
as a representative monophyletic group. We analysed between-individual variation in shape to detect functional
modules and within-individuals the asymmetric component of variation in shape to detect developmental modules.
At the evolutionary level, 17 species from five genera were analysed in phylogenetic context and taking effects of
allometry on modularity and integration into account. We found that allometry is an important integrating factor
in serially homologous structures. At the static level and after the correction for allometry, functional integration
between the cranium and first vertebrae was weak but statistically significant between all elements, and
developmental integration was significant between the cranium and the atlas and first and second trunk vertebrae.
At the evolutionary level, the cranium, atlas and trunk vertebrae separate as three different modules. Our results
suggest that, at the evolutionary level, morphological integration coincide with regional and functional
differentiation of the axial skeleton. This allows the relatively independent evolution of the cranial skeleton and
the vertebral column, separate of the significant functional and developmental integration at the static level.
PB  - Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca
C3  - XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy
T1  - Мorphological integration of the cranium and axial skeleton in european newts
SP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5527
ER  - 

@conference{
author = "Urošević, Aleksandar and Ajduković, Maja and Arntzen, Jan W and Ivanović, Ana",
year = "2019",
abstract = "Using micro-CT scanning and 3D geometric morphometrics of newt craniums and axial skeletons (first
three vertebrae) we explored the pattern of morphological integration. We tested if i) directly connected serially
homologous structures are more integrated than separated ones and ii) morphological integration coincides with
regional differentiation. We applied a multilevel approach by analyzing patterns of integration at static and
evolutionary levels, i.e., within and between species respectively. At the static level we choose the genus Triturus
as a representative monophyletic group. We analysed between-individual variation in shape to detect functional
modules and within-individuals the asymmetric component of variation in shape to detect developmental modules.
At the evolutionary level, 17 species from five genera were analysed in phylogenetic context and taking effects of
allometry on modularity and integration into account. We found that allometry is an important integrating factor
in serially homologous structures. At the static level and after the correction for allometry, functional integration
between the cranium and first vertebrae was weak but statistically significant between all elements, and
developmental integration was significant between the cranium and the atlas and first and second trunk vertebrae.
At the evolutionary level, the cranium, atlas and trunk vertebrae separate as three different modules. Our results
suggest that, at the evolutionary level, morphological integration coincide with regional and functional
differentiation of the axial skeleton. This allows the relatively independent evolution of the cranial skeleton and
the vertebral column, separate of the significant functional and developmental integration at the static level.",
publisher = "Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca",
journal = "XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy",
title = "Мorphological integration of the cranium and axial skeleton in european newts",
pages = "142",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5527"
}

Urošević, A., Ajduković, M., Arntzen, J. W.,& Ivanović, A.. (2019). Мorphological integration of the cranium and axial skeleton in european newts. in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy
Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca., 142.
https://hdl.handle.net/21.15107/rcub_ibiss_5527

Urošević A, Ajduković M, Arntzen JW, Ivanović A. Мorphological integration of the cranium and axial skeleton in european newts. in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy. 2019;:142.
https://hdl.handle.net/21.15107/rcub_ibiss_5527 .

Urošević, Aleksandar, Ajduković, Maja, Arntzen, Jan W, Ivanović, Ana, "Мorphological integration of the cranium and axial skeleton in european newts" in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy (2019):142,
https://hdl.handle.net/21.15107/rcub_ibiss_5527 .

TY  - JOUR
AU  - Urošević, Aleksandar
AU  - Ljubisavljević, Katarina
AU  - Ivanović, Ana
PY  - 2019
UR  - http://doi.wiley.com/10.1111/jzs.12245
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3237
AB  - Different factors and processes that produce phenotypic variation at the individual, population, or interspecific level can influence or alter the covariance structure among morphological traits. Therefore, studies of the patterns of integration and modularity at multiple levels—static, ontogenetic, and evolutionary, can provide invaluable data on underlying factors and processes that structured morphological variation, directed, or constrained evolutionary changes. Our dataset, consisting of cranium shape data for 14 lizard species from the family Lacertidae, with substantial samples of hatchlings and adults along with their inferred evolutionary relationships, enabled us to assess modularity and morphological integration at all three levels. Five, not mutually exclusive modularity hypotheses of lizard cranium, were tested, and the effects of allometry on intensity and the pattern of integration and modularity were estimated. We used geometric morphometrics to extract symmetric and asymmetric, as well as allometric and nonallometric, components of shape variation. At the static level, firm confirmation of cranial modularity was found for hypotheses which separate anterior and posterior functional compartments of the skull. At the ontogenetic level, two alternative hypotheses (the “anteroposterior” and “neurodermatocranial” hypotheses) of ventral cranial modularity were confirmed. At the evolutionary level, the “neurodermatocranial” hypothesis was confirmed for the ventral cranium, which is in accordance with the pattern observed at the ontogenetic level. The observed pattern of static modularity could be driven by functional demands and can be regarded as adaptive. Ontogenetic modularity and evolutionary modularity show the same developmental origin, indicating conservatism of modularity patterns driven by developmental constraints.
T2  - Journal of Zoological Systematics and Evolutionary Research
T2  - Journal of Zoological Systematics and Evolutionary Research
T1  - Multilevel assessment of the Lacertid lizard cranial modularity
IS  - 1
VL  - 57
DO  - 10.1111/jzs.12245
SP  - 145
EP  - 158
ER  - 

@article{
author = "Urošević, Aleksandar and Ljubisavljević, Katarina and Ivanović, Ana",
year = "2019",
abstract = "Different factors and processes that produce phenotypic variation at the individual, population, or interspecific level can influence or alter the covariance structure among morphological traits. Therefore, studies of the patterns of integration and modularity at multiple levels—static, ontogenetic, and evolutionary, can provide invaluable data on underlying factors and processes that structured morphological variation, directed, or constrained evolutionary changes. Our dataset, consisting of cranium shape data for 14 lizard species from the family Lacertidae, with substantial samples of hatchlings and adults along with their inferred evolutionary relationships, enabled us to assess modularity and morphological integration at all three levels. Five, not mutually exclusive modularity hypotheses of lizard cranium, were tested, and the effects of allometry on intensity and the pattern of integration and modularity were estimated. We used geometric morphometrics to extract symmetric and asymmetric, as well as allometric and nonallometric, components of shape variation. At the static level, firm confirmation of cranial modularity was found for hypotheses which separate anterior and posterior functional compartments of the skull. At the ontogenetic level, two alternative hypotheses (the “anteroposterior” and “neurodermatocranial” hypotheses) of ventral cranial modularity were confirmed. At the evolutionary level, the “neurodermatocranial” hypothesis was confirmed for the ventral cranium, which is in accordance with the pattern observed at the ontogenetic level. The observed pattern of static modularity could be driven by functional demands and can be regarded as adaptive. Ontogenetic modularity and evolutionary modularity show the same developmental origin, indicating conservatism of modularity patterns driven by developmental constraints.",
journal = "Journal of Zoological Systematics and Evolutionary Research, Journal of Zoological Systematics and Evolutionary Research",
title = "Multilevel assessment of the Lacertid lizard cranial modularity",
number = "1",
volume = "57",
doi = "10.1111/jzs.12245",
pages = "145-158"
}

Urošević, A., Ljubisavljević, K.,& Ivanović, A.. (2019). Multilevel assessment of the Lacertid lizard cranial modularity. in Journal of Zoological Systematics and Evolutionary Research, 57(1), 145-158.
https://doi.org/10.1111/jzs.12245

Urošević A, Ljubisavljević K, Ivanović A. Multilevel assessment of the Lacertid lizard cranial modularity. in Journal of Zoological Systematics and Evolutionary Research. 2019;57(1):145-158.
doi:10.1111/jzs.12245 .

Urošević, Aleksandar, Ljubisavljević, Katarina, Ivanović, Ana, "Multilevel assessment of the Lacertid lizard cranial modularity" in Journal of Zoological Systematics and Evolutionary Research, 57, no. 1 (2019):145-158,
https://doi.org/10.1111/jzs.12245 . .

TY  - CONF
AU  - Urošević, Aleksandar
AU  - Ajduković, Maja
AU  - Jan Willem, Arntzen
AU  - Ivanović, Ana
PY  - 2019
UR  - http://seh-congress-2019.unipv.it/wp-content/uploads/2019/08/SEHXX-abstract-book-3.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3496
AB  - Using micro-CT scanning and 3D geometric morphometrics of newt craniums and axial skeletons (first three vertebrae) we explored the pattern of morphological integration. We tested if i) directly connected serially homologous structures are more integrated than separated ones and ii) morphological integration coincides with regional differentiation. We applied a multilevel approach by analyzing patterns of integration at static and evolutionary levels, i.e., within and between species respectively. At the static level we choose the genus Triturus as a representative monophyletic group. We analysed between-individual variation in shape to detect functional modules and within-individuals the asymmetric component of variation in shape to detect developmental modules. At the evolutionary level, 17 species from five genera were analysed in phylogenetic context and taking effects of allometry on modularity and integration into account. We found that allometry is an important integrating factor in serially homologous structures. At the static level and after the correction for allometry, functional integration between the cranium and first vertebrae was weak but statistically significant between all elements, and developmental integration was significant between the cranium and the atlas and first and second trunk vertebrae. At the evolutionary level, the cranium, atlas and trunk vertebrae separate as three different modules. Our results suggest that, at the evolutionary level, morphological integration coincide with regional and functional differentiation of the axial skeleton. This allows the relatively independent evolution of the cranial skeleton and the vertebral column, separate of the significant functional and developmental integration at the static level.
PB  - Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca
C3  - XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy
T1  - Morphological integration of the cranium and axial skeleton In European newts
SP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3496
ER  - 

@conference{
author = "Urošević, Aleksandar and Ajduković, Maja and Jan Willem, Arntzen and Ivanović, Ana",
year = "2019",
abstract = "Using micro-CT scanning and 3D geometric morphometrics of newt craniums and axial skeletons (first three vertebrae) we explored the pattern of morphological integration. We tested if i) directly connected serially homologous structures are more integrated than separated ones and ii) morphological integration coincides with regional differentiation. We applied a multilevel approach by analyzing patterns of integration at static and evolutionary levels, i.e., within and between species respectively. At the static level we choose the genus Triturus as a representative monophyletic group. We analysed between-individual variation in shape to detect functional modules and within-individuals the asymmetric component of variation in shape to detect developmental modules. At the evolutionary level, 17 species from five genera were analysed in phylogenetic context and taking effects of allometry on modularity and integration into account. We found that allometry is an important integrating factor in serially homologous structures. At the static level and after the correction for allometry, functional integration between the cranium and first vertebrae was weak but statistically significant between all elements, and developmental integration was significant between the cranium and the atlas and first and second trunk vertebrae. At the evolutionary level, the cranium, atlas and trunk vertebrae separate as three different modules. Our results suggest that, at the evolutionary level, morphological integration coincide with regional and functional differentiation of the axial skeleton. This allows the relatively independent evolution of the cranial skeleton and the vertebral column, separate of the significant functional and developmental integration at the static level.",
publisher = "Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca",
journal = "XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy",
title = "Morphological integration of the cranium and axial skeleton In European newts",
pages = "142",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3496"
}

Urošević, A., Ajduković, M., Jan Willem, A.,& Ivanović, A.. (2019). Morphological integration of the cranium and axial skeleton In European newts. in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy
Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca., 142.
https://hdl.handle.net/21.15107/rcub_ibiss_3496

Urošević A, Ajduković M, Jan Willem A, Ivanović A. Morphological integration of the cranium and axial skeleton In European newts. in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy. 2019;:142.
https://hdl.handle.net/21.15107/rcub_ibiss_3496 .

Urošević, Aleksandar, Ajduković, Maja, Jan Willem, Arntzen, Ivanović, Ana, "Morphological integration of the cranium and axial skeleton In European newts" in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy (2019):142,
https://hdl.handle.net/21.15107/rcub_ibiss_3496 .

TY  - JOUR
AU  - Arntzen, Jan W.
AU  - Üzüm, Nazan
AU  - Ajduković, Maja
AU  - Ivanović, Ana
AU  - Wielstra, Ben
PY  - 2018
UR  - https://peerj.com/articles/5317
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3120
AB  - Relationships between phylogenetic relatedness, hybrid zone spatial structure, the amount of interspecific gene flow and population demography were investigated, with the newt genus Triturus as a model system. In earlier work, a bimodal hybrid zone of two distantly related species combined low interspecific gene flow with hybrid sterility and heterosis was documented. Apart from that, a suite of unimodal hybrid zones in closely related Triturus showed more or less extensive introgressive hybridization with no evidence for heterosis. We here report on population demography and interspecific gene flow in two Triturus species ( T. macedonicus and T. ivanbureschi in Serbia). These are two that are moderately related, engage in a heterogeneous uni-/bimodal hybrid zone and hence represent an intermediate situation. This study used 13 diagnostic nuclear genetic markers in a population at the species contact zone. This showed that all individuals were hybrids, with no parentals detected. Age, size and longevity and the estimated growth curves are not exceeding that of the parental species, so that we conclude the absence of heterosis in T. macedonicus – T. ivanbureschi . Observations across the genus support the hypothesis that fertile hybrids allocate resources to reproduction and infertile hybrids allocate resources to growth. Several Triturus species hybrid zones not yet studied allow the testing of this hypothesis.
T2  - PeerJ
T1  - Absence of heterosis in hybrid crested newts
VL  - 6
DO  - 10.7717/peerj.5317
SP  - e5317
ER  - 

@article{
author = "Arntzen, Jan W. and Üzüm, Nazan and Ajduković, Maja and Ivanović, Ana and Wielstra, Ben",
year = "2018",
abstract = "Relationships between phylogenetic relatedness, hybrid zone spatial structure, the amount of interspecific gene flow and population demography were investigated, with the newt genus Triturus as a model system. In earlier work, a bimodal hybrid zone of two distantly related species combined low interspecific gene flow with hybrid sterility and heterosis was documented. Apart from that, a suite of unimodal hybrid zones in closely related Triturus showed more or less extensive introgressive hybridization with no evidence for heterosis. We here report on population demography and interspecific gene flow in two Triturus species ( T. macedonicus and T. ivanbureschi in Serbia). These are two that are moderately related, engage in a heterogeneous uni-/bimodal hybrid zone and hence represent an intermediate situation. This study used 13 diagnostic nuclear genetic markers in a population at the species contact zone. This showed that all individuals were hybrids, with no parentals detected. Age, size and longevity and the estimated growth curves are not exceeding that of the parental species, so that we conclude the absence of heterosis in T. macedonicus – T. ivanbureschi . Observations across the genus support the hypothesis that fertile hybrids allocate resources to reproduction and infertile hybrids allocate resources to growth. Several Triturus species hybrid zones not yet studied allow the testing of this hypothesis.",
journal = "PeerJ",
title = "Absence of heterosis in hybrid crested newts",
volume = "6",
doi = "10.7717/peerj.5317",
pages = "e5317"
}

Arntzen, J. W., Üzüm, N., Ajduković, M., Ivanović, A.,& Wielstra, B.. (2018). Absence of heterosis in hybrid crested newts. in PeerJ, 6, e5317.
https://doi.org/10.7717/peerj.5317

Arntzen JW, Üzüm N, Ajduković M, Ivanović A, Wielstra B. Absence of heterosis in hybrid crested newts. in PeerJ. 2018;6:e5317.
doi:10.7717/peerj.5317 .

Arntzen, Jan W., Üzüm, Nazan, Ajduković, Maja, Ivanović, Ana, Wielstra, Ben, "Absence of heterosis in hybrid crested newts" in PeerJ, 6 (2018):e5317,
https://doi.org/10.7717/peerj.5317 . .

TY  - CONF
AU  - Danon, Gorana
AU  - Urošević, Aleksandar
AU  - Anđelković, Marko
AU  - Ivanović, Ana
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5213
AB  - We employed methods of landmark based geometric morphometrics to explore ontogeny of sexual dimorphism in pileus shape, as an approximation of sexual dimorphism in head shape of Balkan wall lizard Podarcis tauricus. To estimate the changes in the level and pattern of shape dimorphism over ontogeny, the differences in pileus shape were calculated for subadults and adults. Sex in both subadults and adults was determined according to the presence of the hemipenal bulges. All individuals with snout-vent length less than 52 mm for females and 53 mm for males were classified as subadults. To estimate ontogenetic trajectories of shape changes, the multivariate regression of shape variables on pileus size was performed. The trajectories of shape changes for females and males were compared. We found no significant sexual dimorphism in pileus shape for subadults, while for adults, pronounced sexual dimorphism was found.
In comparison to females, males have relatively narrower pileus and shorter and narrower rostrum. In males, pileus elongation is especially pronounced in the parietal and anterior part of frontoparietal scales. Frontoparietal scales overlay the frontoparietal suture and observed elongation likely helps to compensate for the increased mechanical stress at the important mesokinetic joint. The calculated ontogenetic trajectories of pileus shape in females and males are homogenous, indicating that the main factor leading to sexual dimorphism in pileus shape of Podarcis tauricus are allometric, size related, changes in shape.
PB  - Tel Aviv: The Zoological Society of Israel
C3  - Book of Abstracts: 10th Symposium on the lacertid lizards of the Mediterranean Basin and 2nd Symposium on lizards of the Mediterranean Basin; 2018 Jun 18-21; Tel Aviv, Israel
T1  - Late ontogeny of sexual dimorphism in pileus shape: a case study of Podarcis tauricus
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5213
ER  - 

@conference{
author = "Danon, Gorana and Urošević, Aleksandar and Anđelković, Marko and Ivanović, Ana",
year = "2018",
abstract = "We employed methods of landmark based geometric morphometrics to explore ontogeny of sexual dimorphism in pileus shape, as an approximation of sexual dimorphism in head shape of Balkan wall lizard Podarcis tauricus. To estimate the changes in the level and pattern of shape dimorphism over ontogeny, the differences in pileus shape were calculated for subadults and adults. Sex in both subadults and adults was determined according to the presence of the hemipenal bulges. All individuals with snout-vent length less than 52 mm for females and 53 mm for males were classified as subadults. To estimate ontogenetic trajectories of shape changes, the multivariate regression of shape variables on pileus size was performed. The trajectories of shape changes for females and males were compared. We found no significant sexual dimorphism in pileus shape for subadults, while for adults, pronounced sexual dimorphism was found.
In comparison to females, males have relatively narrower pileus and shorter and narrower rostrum. In males, pileus elongation is especially pronounced in the parietal and anterior part of frontoparietal scales. Frontoparietal scales overlay the frontoparietal suture and observed elongation likely helps to compensate for the increased mechanical stress at the important mesokinetic joint. The calculated ontogenetic trajectories of pileus shape in females and males are homogenous, indicating that the main factor leading to sexual dimorphism in pileus shape of Podarcis tauricus are allometric, size related, changes in shape.",
publisher = "Tel Aviv: The Zoological Society of Israel",
journal = "Book of Abstracts: 10th Symposium on the lacertid lizards of the Mediterranean Basin and 2nd Symposium on lizards of the Mediterranean Basin; 2018 Jun 18-21; Tel Aviv, Israel",
title = "Late ontogeny of sexual dimorphism in pileus shape: a case study of Podarcis tauricus",
pages = "19",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5213"
}

Danon, G., Urošević, A., Anđelković, M.,& Ivanović, A.. (2018). Late ontogeny of sexual dimorphism in pileus shape: a case study of Podarcis tauricus. in Book of Abstracts: 10th Symposium on the lacertid lizards of the Mediterranean Basin and 2nd Symposium on lizards of the Mediterranean Basin; 2018 Jun 18-21; Tel Aviv, Israel
Tel Aviv: The Zoological Society of Israel., 19.
https://hdl.handle.net/21.15107/rcub_ibiss_5213

Danon G, Urošević A, Anđelković M, Ivanović A. Late ontogeny of sexual dimorphism in pileus shape: a case study of Podarcis tauricus. in Book of Abstracts: 10th Symposium on the lacertid lizards of the Mediterranean Basin and 2nd Symposium on lizards of the Mediterranean Basin; 2018 Jun 18-21; Tel Aviv, Israel. 2018;:19.
https://hdl.handle.net/21.15107/rcub_ibiss_5213 .

Danon, Gorana, Urošević, Aleksandar, Anđelković, Marko, Ivanović, Ana, "Late ontogeny of sexual dimorphism in pileus shape: a case study of Podarcis tauricus" in Book of Abstracts: 10th Symposium on the lacertid lizards of the Mediterranean Basin and 2nd Symposium on lizards of the Mediterranean Basin; 2018 Jun 18-21; Tel Aviv, Israel (2018):19,
https://hdl.handle.net/21.15107/rcub_ibiss_5213 .

TY  - JOUR
AU  - Vučić, Tijana
AU  - Vukov, Tanja
AU  - Tomašević Kolarov, Nataša
AU  - Cvijanović, Milena
AU  - Ivanović, Ana
PY  - 2018
UR  - http://booksandjournals.brillonline.com/content/journals/10.1163/15685381-17000190
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3013
AB  - In amphibians, morphological differentiation and disparity at the larval and post-metamorphic ontogenetic stages can diverge, owing to various contrasting environments and different selective pressures. In the monophyletic clade of nine Triturus newt species, five different morphotypes can be recognized, but information on larval morphology is limited. Here we explore divergence of larval morphology in Triturus ivanbureschi, T. macedonicus, and their F1 hybrids. These two genetically and morphologically distinct crested newt species hybridize in nature and form a relatively wide hybrid zone in the central part of the Balkan Peninsula. Using a geometric morphometric approach and multivariate statistics, we evaluated differences of tail size and shape, colouration pattern, and the presence of a tail filament at the mid-larval stage in larvae reared under controlled laboratory conditions. We chose the tail as the main propulsive organ crucial for locomotion, feeding, and escaping predators. We found that Triturus ivanbureschi and T. macedonicus larvae differ in tail shape, but not in tail size. Two groups of F1 hybrid larvae (obtained from reciprocal crossing) were similar to each other, but differed from the parental species in size and shape of the tail, colouration pattern, and the presence of a tail filament. Our results indicate that, like adults, larvae diverge morphologically and hybrid larvae do not exhibit intermediate morphology of the parental species.
T2  - Amphibia-Reptilia
T1  - The study of larval tail morphology reveals differentiation between two Triturus species and their hybrids
IS  - 1
VL  - 39
DO  - 10.1163/15685381-17000190
SP  - 87
EP  - 97
ER  - 

@article{
author = "Vučić, Tijana and Vukov, Tanja and Tomašević Kolarov, Nataša and Cvijanović, Milena and Ivanović, Ana",
year = "2018",
abstract = "In amphibians, morphological differentiation and disparity at the larval and post-metamorphic ontogenetic stages can diverge, owing to various contrasting environments and different selective pressures. In the monophyletic clade of nine Triturus newt species, five different morphotypes can be recognized, but information on larval morphology is limited. Here we explore divergence of larval morphology in Triturus ivanbureschi, T. macedonicus, and their F1 hybrids. These two genetically and morphologically distinct crested newt species hybridize in nature and form a relatively wide hybrid zone in the central part of the Balkan Peninsula. Using a geometric morphometric approach and multivariate statistics, we evaluated differences of tail size and shape, colouration pattern, and the presence of a tail filament at the mid-larval stage in larvae reared under controlled laboratory conditions. We chose the tail as the main propulsive organ crucial for locomotion, feeding, and escaping predators. We found that Triturus ivanbureschi and T. macedonicus larvae differ in tail shape, but not in tail size. Two groups of F1 hybrid larvae (obtained from reciprocal crossing) were similar to each other, but differed from the parental species in size and shape of the tail, colouration pattern, and the presence of a tail filament. Our results indicate that, like adults, larvae diverge morphologically and hybrid larvae do not exhibit intermediate morphology of the parental species.",
journal = "Amphibia-Reptilia",
title = "The study of larval tail morphology reveals differentiation between two Triturus species and their hybrids",
number = "1",
volume = "39",
doi = "10.1163/15685381-17000190",
pages = "87-97"
}

Vučić, T., Vukov, T., Tomašević Kolarov, N., Cvijanović, M.,& Ivanović, A.. (2018). The study of larval tail morphology reveals differentiation between two Triturus species and their hybrids. in Amphibia-Reptilia, 39(1), 87-97.
https://doi.org/10.1163/15685381-17000190

Vučić T, Vukov T, Tomašević Kolarov N, Cvijanović M, Ivanović A. The study of larval tail morphology reveals differentiation between two Triturus species and their hybrids. in Amphibia-Reptilia. 2018;39(1):87-97.
doi:10.1163/15685381-17000190 .

Vučić, Tijana, Vukov, Tanja, Tomašević Kolarov, Nataša, Cvijanović, Milena, Ivanović, Ana, "The study of larval tail morphology reveals differentiation between two Triturus species and their hybrids" in Amphibia-Reptilia, 39, no. 1 (2018):87-97,
https://doi.org/10.1163/15685381-17000190 . .

TY  - JOUR
AU  - Ajduković, Maja
AU  - Ukropina, Mirela
AU  - Filipović, Branko
AU  - Ivanović, Ana
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0944200617300521
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2900
AB  - Vertebral morphology, development, and evolution have been investigated for many decades, especially in the recent evo-devo era. Nevertheless, comparative data on development and ossification modes within the major tetrapod groups are scarce and frequently suffer from the use of a simplistic approach, resulting in simplistic generalizations about the formation of tetrapod vertebrae. Here, we describe the development and ossification of trunk vertebrae in Triturus ivanbureschi (Salamandridae, Caudata) and compare the results with published data on other related taxa. In so doing, we focus on the modes of ossification and development of the centrum and neural arches by analysing three developmental stages defined by the degree of limb development: stages 47, 52, and 62 according to Glu¨cksohn (1932). Our examination of histological sections through trunk vertebrae enabled us to identify three modes of ossification within single trunk vertebrae: (i) perichordal (direct ossification of the connective tissue surrounding the notochord); (ii) perichondrial (direct ossification of the perichondrium, consisting of cartilage-covering connective tissue), and (iii) endochondral (ossification within the preformed cartilage template). We also noted the presence of intravertebral or notochordal cartilage. Although our results indicate that this cartilage develops within the notochord surrounded by the continuous notochordal sheath, more detailed further studies could shed light on its origin and development.
T2  - Zoology
T1  - Ossification and development of vertebrae in the Balkan crested newt Triturus ivanbureschi (Salamandridae, Caudata)
VL  - 128
DO  - 10.1016/j.zool.2017.10.001
SP  - 164
EP  - 171
ER  - 

@article{
author = "Ajduković, Maja and Ukropina, Mirela and Filipović, Branko and Ivanović, Ana",
year = "2018",
abstract = "Vertebral morphology, development, and evolution have been investigated for many decades, especially in the recent evo-devo era. Nevertheless, comparative data on development and ossification modes within the major tetrapod groups are scarce and frequently suffer from the use of a simplistic approach, resulting in simplistic generalizations about the formation of tetrapod vertebrae. Here, we describe the development and ossification of trunk vertebrae in Triturus ivanbureschi (Salamandridae, Caudata) and compare the results with published data on other related taxa. In so doing, we focus on the modes of ossification and development of the centrum and neural arches by analysing three developmental stages defined by the degree of limb development: stages 47, 52, and 62 according to Glu¨cksohn (1932). Our examination of histological sections through trunk vertebrae enabled us to identify three modes of ossification within single trunk vertebrae: (i) perichordal (direct ossification of the connective tissue surrounding the notochord); (ii) perichondrial (direct ossification of the perichondrium, consisting of cartilage-covering connective tissue), and (iii) endochondral (ossification within the preformed cartilage template). We also noted the presence of intravertebral or notochordal cartilage. Although our results indicate that this cartilage develops within the notochord surrounded by the continuous notochordal sheath, more detailed further studies could shed light on its origin and development.",
journal = "Zoology",
title = "Ossification and development of vertebrae in the Balkan crested newt Triturus ivanbureschi (Salamandridae, Caudata)",
volume = "128",
doi = "10.1016/j.zool.2017.10.001",
pages = "164-171"
}

Ajduković, M., Ukropina, M., Filipović, B.,& Ivanović, A.. (2018). Ossification and development of vertebrae in the Balkan crested newt Triturus ivanbureschi (Salamandridae, Caudata). in Zoology, 128, 164-171.
https://doi.org/10.1016/j.zool.2017.10.001

Ajduković M, Ukropina M, Filipović B, Ivanović A. Ossification and development of vertebrae in the Balkan crested newt Triturus ivanbureschi (Salamandridae, Caudata). in Zoology. 2018;128:164-171.
doi:10.1016/j.zool.2017.10.001 .

Ajduković, Maja, Ukropina, Mirela, Filipović, Branko, Ivanović, Ana, "Ossification and development of vertebrae in the Balkan crested newt Triturus ivanbureschi (Salamandridae, Caudata)" in Zoology, 128 (2018):164-171,
https://doi.org/10.1016/j.zool.2017.10.001 . .

TY  - JOUR
AU  - Cvijanović, Milena
AU  - Üzüm, Nazan
AU  - Ivanović, Ana
AU  - Avci, Aziz
AU  - Özcan, Çiçek Gümüş
AU  - Olgun, Kurtuluş
PY  - 2017
UR  - https://www.thebhs.org/publications/the-herpetological-journal/volume-27-number-1-january-2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2804
AB  - According to Rensch’s rule, sexual size dimorphism (SSD) increases with body size in groups where males are the larger sex and decreases when females are larger. The genus Ommatotriton represents a well-defined lineage with male-biased SSD within a group of Eurasian newts otherwise characterised by females being larger than males. In the present paper, we explore sexual dimorphism in skull size and shape for populations of the banded newt Ommatotriton ophryticus, applying geometric morphometrics to investigate size-dependent allometric shape variation. Sexual dimorphism in skull size was not correlated with the size of males, rejecting Rensch’s rule. Sexual dimorphism in skull shape of O. ophryticus is entirely due to allometric, size-related shape changes between sexes.
T2  - Herpetological Journal
T1  - Variation in skull size and shape in a newt species with male-biased sexual dimorphism
IS  - 1
VL  - 27
SP  - 41
EP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2804
ER  - 

@article{
author = "Cvijanović, Milena and Üzüm, Nazan and Ivanović, Ana and Avci, Aziz and Özcan, Çiçek Gümüş and Olgun, Kurtuluş",
year = "2017",
abstract = "According to Rensch’s rule, sexual size dimorphism (SSD) increases with body size in groups where males are the larger sex and decreases when females are larger. The genus Ommatotriton represents a well-defined lineage with male-biased SSD within a group of Eurasian newts otherwise characterised by females being larger than males. In the present paper, we explore sexual dimorphism in skull size and shape for populations of the banded newt Ommatotriton ophryticus, applying geometric morphometrics to investigate size-dependent allometric shape variation. Sexual dimorphism in skull size was not correlated with the size of males, rejecting Rensch’s rule. Sexual dimorphism in skull shape of O. ophryticus is entirely due to allometric, size-related shape changes between sexes.",
journal = "Herpetological Journal",
title = "Variation in skull size and shape in a newt species with male-biased sexual dimorphism",
number = "1",
volume = "27",
pages = "41-46",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2804"
}

Cvijanović, M., Üzüm, N., Ivanović, A., Avci, A., Özcan, Ç. G.,& Olgun, K.. (2017). Variation in skull size and shape in a newt species with male-biased sexual dimorphism. in Herpetological Journal, 27(1), 41-46.
https://hdl.handle.net/21.15107/rcub_ibiss_2804

Cvijanović M, Üzüm N, Ivanović A, Avci A, Özcan ÇG, Olgun K. Variation in skull size and shape in a newt species with male-biased sexual dimorphism. in Herpetological Journal. 2017;27(1):41-46.
https://hdl.handle.net/21.15107/rcub_ibiss_2804 .

Cvijanović, Milena, Üzüm, Nazan, Ivanović, Ana, Avci, Aziz, Özcan, Çiçek Gümüş, Olgun, Kurtuluş, "Variation in skull size and shape in a newt species with male-biased sexual dimorphism" in Herpetological Journal, 27, no. 1 (2017):41-46,
https://hdl.handle.net/21.15107/rcub_ibiss_2804 .

TY  - GEN
AU  - Tomašević Kolarov, Nataša
AU  - Cvijanović, Milena
AU  - Denoël, Mathieu
AU  - Ivanović, Ana
PY  - 2017
UR  - http://doi.wiley.com/10.1002/jez.b.22758
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2795
AB  - Tetrapod limbs are serially homologous structures that represent a particularly interesting model for studies on morphological integration, i.e. the tendency of developmental systems to produce correlated variation. In newts, limbs develop at an early larval stage and grow continuously, including after the habitat transition from water to land following metamorphosis. However, aquatic and terrestrial environments impose different constraints and locomotor modes that could affect patterns of morphological integration and evolvability. We hypothesize that this would be the case for alternative heterochronic morphs in newts, i.e. aquatic paedomorphs that keep gills at the adult stage and adult metamorphs that are able to disperse on land. To this end, we analyzed patterns and strengths of correlations between homologous skeletal elements of the fore- and hindlimbs as well as among skeletal elements within limbs in both phenotypes in the alpine newt, Ichthyosaura alpestris. Our results showed that metamorphs and paedomorphs had similar, general patterns of limb integration. Partial correlations between homologous limb elements and within limb elements were higher in paedomorphs when compared to metamorphs. A decrease in partial correlation between homologous limb elements in metamorphs is accompanied with a higher evolvability of the terrestrial morph. All these results indicate that environmental demands shaped the patterns of morphological integration of alpine newt limbs and that the observed diversity in correlation structure could be related to a qualitative difference in the modes of locomotion between the morphs.
T2  - Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
T1  - Morphological Integration and Alternative Life History Strategies: A Case Study in a Facultatively Paedomorphic Newt
IS  - 8
VL  - 328
DO  - 10.1002/jez.b.22758
SP  - 737
EP  - 748
ER  - 

@misc{
author = "Tomašević Kolarov, Nataša and Cvijanović, Milena and Denoël, Mathieu and Ivanović, Ana",
year = "2017",
abstract = "Tetrapod limbs are serially homologous structures that represent a particularly interesting model for studies on morphological integration, i.e. the tendency of developmental systems to produce correlated variation. In newts, limbs develop at an early larval stage and grow continuously, including after the habitat transition from water to land following metamorphosis. However, aquatic and terrestrial environments impose different constraints and locomotor modes that could affect patterns of morphological integration and evolvability. We hypothesize that this would be the case for alternative heterochronic morphs in newts, i.e. aquatic paedomorphs that keep gills at the adult stage and adult metamorphs that are able to disperse on land. To this end, we analyzed patterns and strengths of correlations between homologous skeletal elements of the fore- and hindlimbs as well as among skeletal elements within limbs in both phenotypes in the alpine newt, Ichthyosaura alpestris. Our results showed that metamorphs and paedomorphs had similar, general patterns of limb integration. Partial correlations between homologous limb elements and within limb elements were higher in paedomorphs when compared to metamorphs. A decrease in partial correlation between homologous limb elements in metamorphs is accompanied with a higher evolvability of the terrestrial morph. All these results indicate that environmental demands shaped the patterns of morphological integration of alpine newt limbs and that the observed diversity in correlation structure could be related to a qualitative difference in the modes of locomotion between the morphs.",
journal = "Journal of Experimental Zoology Part B: Molecular and Developmental Evolution",
title = "Morphological Integration and Alternative Life History Strategies: A Case Study in a Facultatively Paedomorphic Newt",
number = "8",
volume = "328",
doi = "10.1002/jez.b.22758",
pages = "737-748"
}

Tomašević Kolarov, N., Cvijanović, M., Denoël, M.,& Ivanović, A.. (2017). Morphological Integration and Alternative Life History Strategies: A Case Study in a Facultatively Paedomorphic Newt. in Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, 328(8), 737-748.
https://doi.org/10.1002/jez.b.22758

Tomašević Kolarov N, Cvijanović M, Denoël M, Ivanović A. Morphological Integration and Alternative Life History Strategies: A Case Study in a Facultatively Paedomorphic Newt. in Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. 2017;328(8):737-748.
doi:10.1002/jez.b.22758 .

Tomašević Kolarov, Nataša, Cvijanović, Milena, Denoël, Mathieu, Ivanović, Ana, "Morphological Integration and Alternative Life History Strategies: A Case Study in a Facultatively Paedomorphic Newt" in Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, 328, no. 8 (2017):737-748,
https://doi.org/10.1002/jez.b.22758 . .

TY  - JOUR
AU  - Anđelković, Marko
AU  - Tomović, Ljiljana
AU  - Ivanović, Ana
PY  - 2017
UR  - http://doi.wiley.com/10.1111/jzo.12477
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2776
AB  - Morphological integration, the covariation among phenotypic traits generated by common development and function, has been in the scope of evolutionary research for decades. As a morphological structure with complex development and various functions, the cranial skeleton represents a particularly interesting model for studies on morphological integration. However, most of the empirical investigations were done on akinetic and compact cranial skeletons of mammals. Here, we explore the pattern of integration in the extremely kinetic cranial skeleton of two closely related snake species, Natrix natrix and N. tessellata (Natricinae, Colubridae). In snakes, elements of jaws and palates on the left and right side are not spatially connected or firmly fused, allowing independent motion. Spatial independence of skeletal elements on the left and right side and their functional interconnections with extreme kinetic abilities, provide unique feeding performance in this group of tetrapods. By comparing patterns of symmetric and asymmetric components of variation we analysed cavariation patterns between kinetic and akinetic cranial elements. We tested whether the functionally and spatially connected bones are more integrated than disconnected ones and we examine impact of development and function on the morphological integration. We also explored whether and how allometry affects morphological integration in the snake's skull. Using micro-CT scanning 3D geometric morphometrics we showed strong covariation between the braincase and elements of the feeding apparatus, and that spatially disconnected elements are not more integrated than the connected ones. We also showed that function is the main factor that generates the pattern of morphological integration, because the signal of developmental integration is very weak and probably masked by strong functional integration of skeletal elements. Allometry has a significant impact on the morphological integration, by increasing integration of the skull, particularly integration of the lower jaw bones (compound and dentary), prefrontal, palatine and quadrate with the other skeletal elements. © 2017 The Zoological Society of London.
T2  - Journal of Zoology
T1  - Morphological integration of the kinetic skull in Natrix snakes
DO  - 10.1111/jzo.12477
ER  - 

@article{
author = "Anđelković, Marko and Tomović, Ljiljana and Ivanović, Ana",
year = "2017",
abstract = "Morphological integration, the covariation among phenotypic traits generated by common development and function, has been in the scope of evolutionary research for decades. As a morphological structure with complex development and various functions, the cranial skeleton represents a particularly interesting model for studies on morphological integration. However, most of the empirical investigations were done on akinetic and compact cranial skeletons of mammals. Here, we explore the pattern of integration in the extremely kinetic cranial skeleton of two closely related snake species, Natrix natrix and N. tessellata (Natricinae, Colubridae). In snakes, elements of jaws and palates on the left and right side are not spatially connected or firmly fused, allowing independent motion. Spatial independence of skeletal elements on the left and right side and their functional interconnections with extreme kinetic abilities, provide unique feeding performance in this group of tetrapods. By comparing patterns of symmetric and asymmetric components of variation we analysed cavariation patterns between kinetic and akinetic cranial elements. We tested whether the functionally and spatially connected bones are more integrated than disconnected ones and we examine impact of development and function on the morphological integration. We also explored whether and how allometry affects morphological integration in the snake's skull. Using micro-CT scanning 3D geometric morphometrics we showed strong covariation between the braincase and elements of the feeding apparatus, and that spatially disconnected elements are not more integrated than the connected ones. We also showed that function is the main factor that generates the pattern of morphological integration, because the signal of developmental integration is very weak and probably masked by strong functional integration of skeletal elements. Allometry has a significant impact on the morphological integration, by increasing integration of the skull, particularly integration of the lower jaw bones (compound and dentary), prefrontal, palatine and quadrate with the other skeletal elements. © 2017 The Zoological Society of London.",
journal = "Journal of Zoology",
title = "Morphological integration of the kinetic skull in Natrix snakes",
doi = "10.1111/jzo.12477"
}

Anđelković, M., Tomović, L.,& Ivanović, A.. (2017). Morphological integration of the kinetic skull in Natrix snakes. in Journal of Zoology.
https://doi.org/10.1111/jzo.12477

Anđelković M, Tomović L, Ivanović A. Morphological integration of the kinetic skull in Natrix snakes. in Journal of Zoology. 2017;.
doi:10.1111/jzo.12477 .

Anđelković, Marko, Tomović, Ljiljana, Ivanović, Ana, "Morphological integration of the kinetic skull in Natrix snakes" in Journal of Zoology (2017),
https://doi.org/10.1111/jzo.12477 . .