TY  - JOUR
AU  - Stojković, Dejan
AU  - Drakulić, Danijela
AU  - Schwirtlich, Marija
AU  - Rajčević, Nemanja
AU  - Stevanović, Milena
AU  - Soković, Marina
AU  - Gašić, Uroš
PY  - 2021
UR  - https://www.mdpi.com/1424-8247/14/1/55
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4209
AB  - Anthriscus cerefolium (L.) Hoffm. is a plant traditionally used around the globe since antiquity. Although widely used in many traditional medicines in different cultures, from the scientific point of view it is poorly investigated. Glioblastoma, a tumor type with poor prognosis, is the most common and lethal brain tumor in adults. Current therapeutic strategies for glioblastoma include surgery, radiation and chemotherapy. On the other hand, it has been revealed that patients with cancers are highly susceptible to microbial infections due to the invasive nature of cancer treatment approaches. This study was designed to investigate the chemical profile of herba Anthriscii cerefoli methanolic extract by applying UHPLC-LTQ OrbiTrap MS4 analysis and to analyze its anti-glioblastoma and antimicrobial activities. This study revealed that methanolic extract of herba Anthrisc cerefolii contained phenolic acids and flavonoids, with 32 compounds being identified. Anti-glioblastoma activity was investigated in vitro using A172 glioblastoma cell line. The cytotoxic effects of the extract on A172 cells were compared to the same effect on primary human gingival fibroblast (HGF-1) cells. Decreased rate of proliferation and changes in cell morphology were detected upon treatment of A172 cells with the extract. The antimicrobial activity of extract was tested against Staphylococcus aureus and Candida species. The extract was active against the tested bacterium and yeasts, inhibiting free floating cells and microbial biofilms. This study is the first one to provide a detailed description of the chemical profile of A. cerefolium extract dealing with scientific insights into its anti-glioblastoma and antimicrobial activities.
PB  - MDPI AG
T2  - Pharmaceuticals
T1  - Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions
IS  - 1
VL  - 14
DO  - 10.3390/ph14010055
SP  - 55
ER  - 

@article{
author = "Stojković, Dejan and Drakulić, Danijela and Schwirtlich, Marija and Rajčević, Nemanja and Stevanović, Milena and Soković, Marina and Gašić, Uroš",
year = "2021",
abstract = "Anthriscus cerefolium (L.) Hoffm. is a plant traditionally used around the globe since antiquity. Although widely used in many traditional medicines in different cultures, from the scientific point of view it is poorly investigated. Glioblastoma, a tumor type with poor prognosis, is the most common and lethal brain tumor in adults. Current therapeutic strategies for glioblastoma include surgery, radiation and chemotherapy. On the other hand, it has been revealed that patients with cancers are highly susceptible to microbial infections due to the invasive nature of cancer treatment approaches. This study was designed to investigate the chemical profile of herba Anthriscii cerefoli methanolic extract by applying UHPLC-LTQ OrbiTrap MS4 analysis and to analyze its anti-glioblastoma and antimicrobial activities. This study revealed that methanolic extract of herba Anthrisc cerefolii contained phenolic acids and flavonoids, with 32 compounds being identified. Anti-glioblastoma activity was investigated in vitro using A172 glioblastoma cell line. The cytotoxic effects of the extract on A172 cells were compared to the same effect on primary human gingival fibroblast (HGF-1) cells. Decreased rate of proliferation and changes in cell morphology were detected upon treatment of A172 cells with the extract. The antimicrobial activity of extract was tested against Staphylococcus aureus and Candida species. The extract was active against the tested bacterium and yeasts, inhibiting free floating cells and microbial biofilms. This study is the first one to provide a detailed description of the chemical profile of A. cerefolium extract dealing with scientific insights into its anti-glioblastoma and antimicrobial activities.",
publisher = "MDPI AG",
journal = "Pharmaceuticals",
title = "Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions",
number = "1",
volume = "14",
doi = "10.3390/ph14010055",
pages = "55"
}

Stojković, D., Drakulić, D., Schwirtlich, M., Rajčević, N., Stevanović, M., Soković, M.,& Gašić, U.. (2021). Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions. in Pharmaceuticals
MDPI AG., 14(1), 55.
https://doi.org/10.3390/ph14010055

Stojković D, Drakulić D, Schwirtlich M, Rajčević N, Stevanović M, Soković M, Gašić U. Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions. in Pharmaceuticals. 2021;14(1):55.
doi:10.3390/ph14010055 .

Stojković, Dejan, Drakulić, Danijela, Schwirtlich, Marija, Rajčević, Nemanja, Stevanović, Milena, Soković, Marina, Gašić, Uroš, "Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions" in Pharmaceuticals, 14, no. 1 (2021):55,
https://doi.org/10.3390/ph14010055 . .

TY  - JOUR
AU  - Zaletel, Ivan
AU  - Schwirtlich, Marija
AU  - Perović, Milka
AU  - Jovanović, Mirna
AU  - Stevanović, Milena
AU  - Kanazir, Selma
AU  - Puškaš, Nela
PY  - 2018
UR  - http://www.medra.org/servlet/aliasResolver?alias=iospress&doi=10.3233/JAD-180277
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3149
AB  - Dysregulation of neurogenesis in the subgranular zone (SGZ) of the hippocampal dentate gyrus has been related to cognitive deficits and memory loss in neurodegenerative diseases, such as Alzheimer's disease (AD). Members of the B group of SOX transcription factors play critical roles in regulating neurogenesis in the embryonic and adult nervous system, including maintaining the multipotency, renewal, and cell fate decision of neural stem/progenitor cells. The aim of the present study was to evaluate the expression patterns of selected SOXB proteins in the SGZ, of 8-week-old male and female 5xFAD mice, which represent a transgenic model of AD with a severe and very early development of amyloid pathology. Immunohistochemical analysis showed a significant decrease in the number of cells expressing SOX1, SOX2, and SOX21 transcription factors within the SGZ of 5xFAD mice in comparison to their non-transgenic counterparts which coincidences with reduced number of doublecortin immunoreactive immature neurons found in Tg males. Despite observed changes in expressional pattern of examined SOXB proteins, the proliferative capacity evaluated by the number of Ki-67 immunoreactive cells remained unaffected in transgenic mice of both genders. Based on our results, we suggest that SOXB proteins might be considered as new biomarkers for the detection of early impairments in adult neurogenesis in different animal models or/and new targets in human regenerative medicine.
T2  - Journal of Alzheimer's Disease : JAD
T1  - Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors.
IS  - 3
VL  - 65
DO  - 10.3233/JAD-180277
SP  - 963
EP  - 976
ER  - 

@article{
author = "Zaletel, Ivan and Schwirtlich, Marija and Perović, Milka and Jovanović, Mirna and Stevanović, Milena and Kanazir, Selma and Puškaš, Nela",
year = "2018",
abstract = "Dysregulation of neurogenesis in the subgranular zone (SGZ) of the hippocampal dentate gyrus has been related to cognitive deficits and memory loss in neurodegenerative diseases, such as Alzheimer's disease (AD). Members of the B group of SOX transcription factors play critical roles in regulating neurogenesis in the embryonic and adult nervous system, including maintaining the multipotency, renewal, and cell fate decision of neural stem/progenitor cells. The aim of the present study was to evaluate the expression patterns of selected SOXB proteins in the SGZ, of 8-week-old male and female 5xFAD mice, which represent a transgenic model of AD with a severe and very early development of amyloid pathology. Immunohistochemical analysis showed a significant decrease in the number of cells expressing SOX1, SOX2, and SOX21 transcription factors within the SGZ of 5xFAD mice in comparison to their non-transgenic counterparts which coincidences with reduced number of doublecortin immunoreactive immature neurons found in Tg males. Despite observed changes in expressional pattern of examined SOXB proteins, the proliferative capacity evaluated by the number of Ki-67 immunoreactive cells remained unaffected in transgenic mice of both genders. Based on our results, we suggest that SOXB proteins might be considered as new biomarkers for the detection of early impairments in adult neurogenesis in different animal models or/and new targets in human regenerative medicine.",
journal = "Journal of Alzheimer's Disease : JAD",
title = "Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors.",
number = "3",
volume = "65",
doi = "10.3233/JAD-180277",
pages = "963-976"
}

Zaletel, I., Schwirtlich, M., Perović, M., Jovanović, M., Stevanović, M., Kanazir, S.,& Puškaš, N.. (2018). Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors.. in Journal of Alzheimer's Disease : JAD, 65(3), 963-976.
https://doi.org/10.3233/JAD-180277

Zaletel I, Schwirtlich M, Perović M, Jovanović M, Stevanović M, Kanazir S, Puškaš N. Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors.. in Journal of Alzheimer's Disease : JAD. 2018;65(3):963-976.
doi:10.3233/JAD-180277 .

Zaletel, Ivan, Schwirtlich, Marija, Perović, Milka, Jovanović, Mirna, Stevanović, Milena, Kanazir, Selma, Puškaš, Nela, "Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors." in Journal of Alzheimer's Disease : JAD, 65, no. 3 (2018):963-976,
https://doi.org/10.3233/JAD-180277 . .