TY  - JOUR
AU  - Cingel, Aleksandar
AU  - Savic, Jelena
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan
AU  - Kostic, Miroslav
AU  - Šešlija Jovanović, Darka
AU  - Smigocki, Ann
AU  - Ninković, Slavica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1923
AB  - Plant proteinase inhibitors (PIs) are attractive tools for crop
   improvement and their heterologous expression can enhance insect
   resistance in transgenic plants. PI oryzacystatin II (OCII), isolated
   from rice, showed potential in controlling pests that utilize cysteine
   proteinases for protein digestion. To evaluate the applicability of the
   OCII gene in enhancing plant defence, OCII-transformed potatoes were
   bioassayed for resistance to Colorado potato beetle (Leptinotarsa
   decemlineata Say). Feeding on transformed leaves of potato cultivars
   Desiree and Jelica significantly affected larval growth and development,
   but did not change mortality rates. During the L2 and L3 developmental
   stages larvae consumed the OCII-transformed foliage faster as compared
   to the nontransformed control. Also these larvae reached the prepupal
   stage (end of L4 stage) 2 days earlier than those fed on control leaves.
   However, the total amounts of consumed OCII-transformed leaves were up
   to 23 \% lower than of control, and the maximal weights of prepupal
   larvae were reduced by up to 18 \% as compared to larvae fed on
   nontransformed leaves. The reduction in insect fitness reported in this
   study in combination with other control measures, could lead to improved
   CPB resistance management in potato.
T2  - Transgenic Research
T1  - Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor
IS  - 4
VL  - 24
DO  - 10.1007/s11248-015-9873-9
SP  - 729
EP  - 740
ER  - 

@article{
author = "Cingel, Aleksandar and Savic, Jelena and Vinterhalter, Branka and Vinterhalter, Dragan and Kostic, Miroslav and Šešlija Jovanović, Darka and Smigocki, Ann and Ninković, Slavica",
year = "2015",
abstract = "Plant proteinase inhibitors (PIs) are attractive tools for crop
   improvement and their heterologous expression can enhance insect
   resistance in transgenic plants. PI oryzacystatin II (OCII), isolated
   from rice, showed potential in controlling pests that utilize cysteine
   proteinases for protein digestion. To evaluate the applicability of the
   OCII gene in enhancing plant defence, OCII-transformed potatoes were
   bioassayed for resistance to Colorado potato beetle (Leptinotarsa
   decemlineata Say). Feeding on transformed leaves of potato cultivars
   Desiree and Jelica significantly affected larval growth and development,
   but did not change mortality rates. During the L2 and L3 developmental
   stages larvae consumed the OCII-transformed foliage faster as compared
   to the nontransformed control. Also these larvae reached the prepupal
   stage (end of L4 stage) 2 days earlier than those fed on control leaves.
   However, the total amounts of consumed OCII-transformed leaves were up
   to 23 \% lower than of control, and the maximal weights of prepupal
   larvae were reduced by up to 18 \% as compared to larvae fed on
   nontransformed leaves. The reduction in insect fitness reported in this
   study in combination with other control measures, could lead to improved
   CPB resistance management in potato.",
journal = "Transgenic Research",
title = "Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor",
number = "4",
volume = "24",
doi = "10.1007/s11248-015-9873-9",
pages = "729-740"
}

Cingel, A., Savic, J., Vinterhalter, B., Vinterhalter, D., Kostic, M., Šešlija Jovanović, D., Smigocki, A.,& Ninković, S.. (2015). Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor. in Transgenic Research, 24(4), 729-740.
https://doi.org/10.1007/s11248-015-9873-9

Cingel A, Savic J, Vinterhalter B, Vinterhalter D, Kostic M, Šešlija Jovanović D, Smigocki A, Ninković S. Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor. in Transgenic Research. 2015;24(4):729-740.
doi:10.1007/s11248-015-9873-9 .

Cingel, Aleksandar, Savic, Jelena, Vinterhalter, Branka, Vinterhalter, Dragan, Kostic, Miroslav, Šešlija Jovanović, Darka, Smigocki, Ann, Ninković, Slavica, "Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor" in Transgenic Research, 24, no. 4 (2015):729-740,
https://doi.org/10.1007/s11248-015-9873-9 . .