TY  - JOUR
AU  - Ilić, Marija
AU  - Bugarski-Stanojević, Vanja
AU  - Jovanović, Bogdan
AU  - Stamenković, Gorana
AU  - Zorić, Katarina
AU  - Paunović, Momir
AU  - Crnobrnja-Isailović, Jelka
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6705
AB  - Simple Summary: Rana temporaria is one of the most widespread Palearctic brown frogs, with two
main genetic clades in Europe, geographic spatial pattern of which is insufficiently known. We
analyzed samples from the understudied western and central Balkans to evaluate the haplotype
diversity of widely used 16SrRNA and MT-CYTB mitochondrial gene sequences and to recognize
the contour of a possible contact zone between the main clades. The results revealed a suture
zone between theWestern and Eastern Clades in the central part of the Balkan Peninsula. Overall,
haplotype diversity in the western and central Balkans sample is high. Harboring both main genetic
clades of R. temporaria qualifies the Balkan Peninsula as another important center of species’ genetic
diversity, as well as rich in unique haplotypes.
Abstract: Rana temporaria is one of the most widespread Palearctic brown frogs. We aimed to
clarify distribution pattern of two main genetic clades in the understudied Balkan peninsula by
using 16SrRNA and MT-CYTB sequences, already widely applied in analyses of populations from
other parts of Europe, while focusing on the broad area along the Morava river (central Balkans)
as a known gap in the species distribution. Additionally, we were interested in revealing the
extent of haplotype diversity within the main genetic clades in the Balkans, particularly around
the supposed suture zone. The results revealed a suture zone between the Western and Eastern
Clades in the central part of the Balkan Peninsula. This indicated the existence of a historical
barrier between the Balkan Mountain Belt and geographically close mountains surrounding the
Vlasina Plateau (Rhodope/Serbian–Macedonian Massif). The overall observed haplotype diversity in
populations of R. temporaria from the Balkan Peninsula seems high. Harboring both main genetic
clades of R. temporaria qualifies the Balkan Peninsula as another important center of species’ genetic
diversity, as well as rich in unique haplotypes. This points out the necessity of applying conservation
measures focused on the common European frog populations and habitats in this part of the species’
distribution area.
PB  - Basel: MDPI
T2  - Animals
T1  - Phylogeographic Substructuring in the Southernmost Refugium of the European Common Frog Rana temporaria
IS  - 10
VL  - 14
DO  - 10.3390/ani14101430
SP  - 1430
ER  - 

@article{
author = "Ilić, Marija and Bugarski-Stanojević, Vanja and Jovanović, Bogdan and Stamenković, Gorana and Zorić, Katarina and Paunović, Momir and Crnobrnja-Isailović, Jelka",
year = "2024",
abstract = "Simple Summary: Rana temporaria is one of the most widespread Palearctic brown frogs, with two
main genetic clades in Europe, geographic spatial pattern of which is insufficiently known. We
analyzed samples from the understudied western and central Balkans to evaluate the haplotype
diversity of widely used 16SrRNA and MT-CYTB mitochondrial gene sequences and to recognize
the contour of a possible contact zone between the main clades. The results revealed a suture
zone between theWestern and Eastern Clades in the central part of the Balkan Peninsula. Overall,
haplotype diversity in the western and central Balkans sample is high. Harboring both main genetic
clades of R. temporaria qualifies the Balkan Peninsula as another important center of species’ genetic
diversity, as well as rich in unique haplotypes.
Abstract: Rana temporaria is one of the most widespread Palearctic brown frogs. We aimed to
clarify distribution pattern of two main genetic clades in the understudied Balkan peninsula by
using 16SrRNA and MT-CYTB sequences, already widely applied in analyses of populations from
other parts of Europe, while focusing on the broad area along the Morava river (central Balkans)
as a known gap in the species distribution. Additionally, we were interested in revealing the
extent of haplotype diversity within the main genetic clades in the Balkans, particularly around
the supposed suture zone. The results revealed a suture zone between the Western and Eastern
Clades in the central part of the Balkan Peninsula. This indicated the existence of a historical
barrier between the Balkan Mountain Belt and geographically close mountains surrounding the
Vlasina Plateau (Rhodope/Serbian–Macedonian Massif). The overall observed haplotype diversity in
populations of R. temporaria from the Balkan Peninsula seems high. Harboring both main genetic
clades of R. temporaria qualifies the Balkan Peninsula as another important center of species’ genetic
diversity, as well as rich in unique haplotypes. This points out the necessity of applying conservation
measures focused on the common European frog populations and habitats in this part of the species’
distribution area.",
publisher = "Basel: MDPI",
journal = "Animals",
title = "Phylogeographic Substructuring in the Southernmost Refugium of the European Common Frog Rana temporaria",
number = "10",
volume = "14",
doi = "10.3390/ani14101430",
pages = "1430"
}

Ilić, M., Bugarski-Stanojević, V., Jovanović, B., Stamenković, G., Zorić, K., Paunović, M.,& Crnobrnja-Isailović, J.. (2024). Phylogeographic Substructuring in the Southernmost Refugium of the European Common Frog Rana temporaria. in Animals
Basel: MDPI., 14(10), 1430.
https://doi.org/10.3390/ani14101430

Ilić M, Bugarski-Stanojević V, Jovanović B, Stamenković G, Zorić K, Paunović M, Crnobrnja-Isailović J. Phylogeographic Substructuring in the Southernmost Refugium of the European Common Frog Rana temporaria. in Animals. 2024;14(10):1430.
doi:10.3390/ani14101430 .

Ilić, Marija, Bugarski-Stanojević, Vanja, Jovanović, Bogdan, Stamenković, Gorana, Zorić, Katarina, Paunović, Momir, Crnobrnja-Isailović, Jelka, "Phylogeographic Substructuring in the Southernmost Refugium of the European Common Frog Rana temporaria" in Animals, 14, no. 10 (2024):1430,
https://doi.org/10.3390/ani14101430 . .

TY  - JOUR
AU  - Bugarski-Stanojević, Vanja
AU  - Đokić, Marko
AU  - Stamenković, Gorana
AU  - Barišić Klisarić, Nataša
AU  - Stojković, Oliver
AU  - Jojić, Vida
AU  - Savić, Ivo
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6587
AB  - Blind mole rats (genus Nannospalax) attract a great deal of attention because of their cancer resistance and longevity. Due to the high rate of chromosome rearrangements, 74 Nannospalax chromosomal forms have been discovered. The convergence of their external morphology complicates their taxonomy, and many cryptic species remain unrecognized. Thus, the European N. leucodon supersp. is listed in the IUCN Red List of Threatened Species with “Data Deficient” status. It is crucial for the conservation of biodiversity to clarify its taxonomy, to recognize each cryptic species, and assign to them the correct conservation status. Of the more than 20 chromosomal forms described within N. leucodon, five cryptic species occur in Serbia. The most threatened among them—N. l. syrmiensis, described and named 50 years ago in the regions of Srem, Belgrade and Mačva—has been declared extinct in the literature, which may have negative consequences for the conservation of wildlife genetic diversity. Through five years of fieldwork and comparison of 16SrRNA and MT-CYTB gene segments between old, archived teeth and recently collected material, we show that N. l. syrmiensis is not extinct. However, its habitat has been fragmented and reduced, owing primarily to anthropogenic impact. Therefore, detailed surveillance, population-structure studies, risk assessment, and appropriate conservation measures are needed.
PB  - Basel: MDPI
T2  - Animals
T1  - A Cryptic Subterranean Mammal Species, the Lesser Blind Mole Rat (Nannospalax leucodon syrmiensis)—Retreated but Not Extinct
IS  - 5
VL  - 14
DO  - 10.3390/ani14050774
SP  - 774
ER  - 

@article{
author = "Bugarski-Stanojević, Vanja and Đokić, Marko and Stamenković, Gorana and Barišić Klisarić, Nataša and Stojković, Oliver and Jojić, Vida and Savić, Ivo",
year = "2024",
abstract = "Blind mole rats (genus Nannospalax) attract a great deal of attention because of their cancer resistance and longevity. Due to the high rate of chromosome rearrangements, 74 Nannospalax chromosomal forms have been discovered. The convergence of their external morphology complicates their taxonomy, and many cryptic species remain unrecognized. Thus, the European N. leucodon supersp. is listed in the IUCN Red List of Threatened Species with “Data Deficient” status. It is crucial for the conservation of biodiversity to clarify its taxonomy, to recognize each cryptic species, and assign to them the correct conservation status. Of the more than 20 chromosomal forms described within N. leucodon, five cryptic species occur in Serbia. The most threatened among them—N. l. syrmiensis, described and named 50 years ago in the regions of Srem, Belgrade and Mačva—has been declared extinct in the literature, which may have negative consequences for the conservation of wildlife genetic diversity. Through five years of fieldwork and comparison of 16SrRNA and MT-CYTB gene segments between old, archived teeth and recently collected material, we show that N. l. syrmiensis is not extinct. However, its habitat has been fragmented and reduced, owing primarily to anthropogenic impact. Therefore, detailed surveillance, population-structure studies, risk assessment, and appropriate conservation measures are needed.",
publisher = "Basel: MDPI",
journal = "Animals",
title = "A Cryptic Subterranean Mammal Species, the Lesser Blind Mole Rat (Nannospalax leucodon syrmiensis)—Retreated but Not Extinct",
number = "5",
volume = "14",
doi = "10.3390/ani14050774",
pages = "774"
}

Bugarski-Stanojević, V., Đokić, M., Stamenković, G., Barišić Klisarić, N., Stojković, O., Jojić, V.,& Savić, I.. (2024). A Cryptic Subterranean Mammal Species, the Lesser Blind Mole Rat (Nannospalax leucodon syrmiensis)—Retreated but Not Extinct. in Animals
Basel: MDPI., 14(5), 774.
https://doi.org/10.3390/ani14050774

Bugarski-Stanojević V, Đokić M, Stamenković G, Barišić Klisarić N, Stojković O, Jojić V, Savić I. A Cryptic Subterranean Mammal Species, the Lesser Blind Mole Rat (Nannospalax leucodon syrmiensis)—Retreated but Not Extinct. in Animals. 2024;14(5):774.
doi:10.3390/ani14050774 .

Bugarski-Stanojević, Vanja, Đokić, Marko, Stamenković, Gorana, Barišić Klisarić, Nataša, Stojković, Oliver, Jojić, Vida, Savić, Ivo, "A Cryptic Subterranean Mammal Species, the Lesser Blind Mole Rat (Nannospalax leucodon syrmiensis)—Retreated but Not Extinct" in Animals, 14, no. 5 (2024):774,
https://doi.org/10.3390/ani14050774 . .

TY  - JOUR
AU  - Jojić, Vida
AU  - Bajić, Aleksandar
AU  - Barišić Klisarić, Nataša
AU  - Bugarski-Stanojević, Vanja
AU  - Snoj, Aleš
AU  - Miljanović, Branko
AU  - Askeyev, Oleg
AU  - Askeyev, Igor
AU  - Marić, Saša
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6390
AB  - This is a preliminary and exploratory study of cranial variation in European populations of grayling. We
investigated the correspondence between size/shape variation of the dorsal (dc), ventral (vc) and occipital
(oc) cranium and phylogenetic relationships (inferred from mitochondrial control region – mtDNA cr
and microsatellite dna data) of six grayling populations: three from Balkan phylogenetic clade and two
from Caspian phylogenetic clade of the European grayling Thymallus thymallus and one population of
the Adriatic grayling Thymallus aeliani, which until recently was considered the Adriatic phylogenetic
clade of T. thymallus. Significant size and shape differences were found between populations in all three
cranial views. However, significant size-related shape variation (allometry) was found for dc and vc, but
not for oc. The size variation of each cranial view does not contain phylogenetic signal, but size variation
of oc is consistent with genetic variation inferred from microsatellite dna. Regarding shape variation, a
significant phylogenetic signal was detected only for oc, and only the shape variation of oc is consistent
with the genetic variation inferred from the mtDNA cr. Moreover, the Adriatic grayling T. aeliani (Soča
population) was clearly separated from the three T. thymallus populations of the Balkan phylogenetic
clade and the two T. thymallus populations of the Caspian phylogenetic clade only at the level of oc. Thus,
our results suggest that different cranial regions differ in allometry, reflect phylo(genetic) relationships
differently, and exhibit differences in ecophenotypic plasticity, with oc seeming best suited to represent
the phylogenetic relationships of the grayling populations studied.
PB  - Leiden: Brill
T2  - Contributions to Zoology
T1  - Exploring the phylogenetic signal in the cranial variation of European populations of grayling (Actinopterygii, Salmonidae)
IS  - 5
VL  - 92
DO  - 10.1163/18759866-bja10051
SP  - 510
EP  - 532
ER  - 

@article{
author = "Jojić, Vida and Bajić, Aleksandar and Barišić Klisarić, Nataša and Bugarski-Stanojević, Vanja and Snoj, Aleš and Miljanović, Branko and Askeyev, Oleg and Askeyev, Igor and Marić, Saša",
year = "2023",
abstract = "This is a preliminary and exploratory study of cranial variation in European populations of grayling. We
investigated the correspondence between size/shape variation of the dorsal (dc), ventral (vc) and occipital
(oc) cranium and phylogenetic relationships (inferred from mitochondrial control region – mtDNA cr
and microsatellite dna data) of six grayling populations: three from Balkan phylogenetic clade and two
from Caspian phylogenetic clade of the European grayling Thymallus thymallus and one population of
the Adriatic grayling Thymallus aeliani, which until recently was considered the Adriatic phylogenetic
clade of T. thymallus. Significant size and shape differences were found between populations in all three
cranial views. However, significant size-related shape variation (allometry) was found for dc and vc, but
not for oc. The size variation of each cranial view does not contain phylogenetic signal, but size variation
of oc is consistent with genetic variation inferred from microsatellite dna. Regarding shape variation, a
significant phylogenetic signal was detected only for oc, and only the shape variation of oc is consistent
with the genetic variation inferred from the mtDNA cr. Moreover, the Adriatic grayling T. aeliani (Soča
population) was clearly separated from the three T. thymallus populations of the Balkan phylogenetic
clade and the two T. thymallus populations of the Caspian phylogenetic clade only at the level of oc. Thus,
our results suggest that different cranial regions differ in allometry, reflect phylo(genetic) relationships
differently, and exhibit differences in ecophenotypic plasticity, with oc seeming best suited to represent
the phylogenetic relationships of the grayling populations studied.",
publisher = "Leiden: Brill",
journal = "Contributions to Zoology",
title = "Exploring the phylogenetic signal in the cranial variation of European populations of grayling (Actinopterygii, Salmonidae)",
number = "5",
volume = "92",
doi = "10.1163/18759866-bja10051",
pages = "510-532"
}

Jojić, V., Bajić, A., Barišić Klisarić, N., Bugarski-Stanojević, V., Snoj, A., Miljanović, B., Askeyev, O., Askeyev, I.,& Marić, S.. (2023). Exploring the phylogenetic signal in the cranial variation of European populations of grayling (Actinopterygii, Salmonidae). in Contributions to Zoology
Leiden: Brill., 92(5), 510-532.
https://doi.org/10.1163/18759866-bja10051

Jojić V, Bajić A, Barišić Klisarić N, Bugarski-Stanojević V, Snoj A, Miljanović B, Askeyev O, Askeyev I, Marić S. Exploring the phylogenetic signal in the cranial variation of European populations of grayling (Actinopterygii, Salmonidae). in Contributions to Zoology. 2023;92(5):510-532.
doi:10.1163/18759866-bja10051 .

Jojić, Vida, Bajić, Aleksandar, Barišić Klisarić, Nataša, Bugarski-Stanojević, Vanja, Snoj, Aleš, Miljanović, Branko, Askeyev, Oleg, Askeyev, Igor, Marić, Saša, "Exploring the phylogenetic signal in the cranial variation of European populations of grayling (Actinopterygii, Salmonidae)" in Contributions to Zoology, 92, no. 5 (2023):510-532,
https://doi.org/10.1163/18759866-bja10051 . .

TY  - JOUR
AU  - Penezić, Aleksandra
AU  - Kuručki, Milica
AU  - Bogdanović, Neda
AU  - Pantelić, Ilija
AU  - Bugarski-Stanojević, Vanja
AU  - Ćirović, Duško
PY  - 2022
UR  - https://link.springer.com/10.1007/s11686-022-00567-9
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4986
AB  - PURPOSE The knowledge of heartworm disease in free ranging wild canid populations is limited. As it is very difficult to monitor this disease in live animals, sporadic findings are mostly obtained by examining culled individuals of game species. METHODS As a part of a broader study on jackal (Canis aureus) ecology in Serbia, the necropsy of legally hunted animals was performed in 2020. RESULTS Two cases of heartworm infection with unusually located adult heartworms were diagnosed. The first case is an adult female jackal harvested in the vicinity of the capital city of Belgrade. Three adult specimens of Dirofilaria immitis were found in the right atrium of the heart, and nine adult specimens were located at an atypical site, in the vena cava caudalis. Very similar, the second case was in an adult female jackal harvested in the vicinity of Bački Monoštor. In this case, six adult specimens of D. immitis were found in the right atrium of the heart and six more adult specimens in the vena cava caudalis. CONCLUSION Although this nematode generally resides in the pulmonary arteries and the right ventricle of the heart, rare findings in other large blood vessels can occur. To the best of our knowledge, these are the first and only two confirmed cases of adult D. immitis found in vena cava caudalis in jackals in Europe.
PB  - Cham: Springer
T2  - Acta Parasitologica
T1  - Heartworm Disease in Jackals: Unusual Location of Dirofilaria immitis.
VL  - 67
DO  - 10.1007/s11686-022-00567-9
SP  - 1412
EP  - 1415
ER  - 

@article{
author = "Penezić, Aleksandra and Kuručki, Milica and Bogdanović, Neda and Pantelić, Ilija and Bugarski-Stanojević, Vanja and Ćirović, Duško",
year = "2022",
abstract = "PURPOSE The knowledge of heartworm disease in free ranging wild canid populations is limited. As it is very difficult to monitor this disease in live animals, sporadic findings are mostly obtained by examining culled individuals of game species. METHODS As a part of a broader study on jackal (Canis aureus) ecology in Serbia, the necropsy of legally hunted animals was performed in 2020. RESULTS Two cases of heartworm infection with unusually located adult heartworms were diagnosed. The first case is an adult female jackal harvested in the vicinity of the capital city of Belgrade. Three adult specimens of Dirofilaria immitis were found in the right atrium of the heart, and nine adult specimens were located at an atypical site, in the vena cava caudalis. Very similar, the second case was in an adult female jackal harvested in the vicinity of Bački Monoštor. In this case, six adult specimens of D. immitis were found in the right atrium of the heart and six more adult specimens in the vena cava caudalis. CONCLUSION Although this nematode generally resides in the pulmonary arteries and the right ventricle of the heart, rare findings in other large blood vessels can occur. To the best of our knowledge, these are the first and only two confirmed cases of adult D. immitis found in vena cava caudalis in jackals in Europe.",
publisher = "Cham: Springer",
journal = "Acta Parasitologica",
title = "Heartworm Disease in Jackals: Unusual Location of Dirofilaria immitis.",
volume = "67",
doi = "10.1007/s11686-022-00567-9",
pages = "1412-1415"
}

Penezić, A., Kuručki, M., Bogdanović, N., Pantelić, I., Bugarski-Stanojević, V.,& Ćirović, D.. (2022). Heartworm Disease in Jackals: Unusual Location of Dirofilaria immitis.. in Acta Parasitologica
Cham: Springer., 67, 1412-1415.
https://doi.org/10.1007/s11686-022-00567-9

Penezić A, Kuručki M, Bogdanović N, Pantelić I, Bugarski-Stanojević V, Ćirović D. Heartworm Disease in Jackals: Unusual Location of Dirofilaria immitis.. in Acta Parasitologica. 2022;67:1412-1415.
doi:10.1007/s11686-022-00567-9 .

Penezić, Aleksandra, Kuručki, Milica, Bogdanović, Neda, Pantelić, Ilija, Bugarski-Stanojević, Vanja, Ćirović, Duško, "Heartworm Disease in Jackals: Unusual Location of Dirofilaria immitis." in Acta Parasitologica, 67 (2022):1412-1415,
https://doi.org/10.1007/s11686-022-00567-9 . .

TY  - JOUR
AU  - Bugarski-Stanojević, Vanja
AU  - Stamenković, Gorana
AU  - Jojić, Vida
AU  - Ćosić, Nada
AU  - Ćirović, Duško
AU  - Stojković, Oliver
AU  - Veličković, Jelena
AU  - Savić, Ivo
PY  - 2022
UR  - https://www.mdpi.com/2076-2615/12/9/1097
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9105853
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4964
AB  - We explored the cryptic speciation of the Nannospalax leucodon species complex, characterised by intense karyotype evolution and reduced phenotypic variability that has produced different lineages, out of which 25 are described as chromosomal forms (CFs), so many cryptic species remain unnoticed. Although some of them should be classified as threatened, they lack the official nomenclature necessary to be involved in conservation strategies. Reproductive isolation between seven CFs has previously been demonstrated. To investigate the amount and dynamics of genetic discrepancy that follows chromosomal changes, infer speciation levels, and obtain phylogenetic patterns, we analysed mitochondrial 16S rRNA and MT-CYTB nucleotide polymorphism among 17 CFs-the highest number studied so far. Phylogenetic trees delineated 11 CFs as separate clades. Evolutionary divergence values overlapped with acknowledged higher taxonomic categories, or sometimes exceeded them. The fact that CFs with higher 2n are evolutionary older corresponds to the fusion hypothesis of Nannospalax karyotype evolution. To participate in conservation strategies, N. leucodon classification should follow the biological species concept, and proposed cryptic species should be formally named, despite a lack of classical morphometric discrepancy. We draw attention towards the syrmiensis and montanosyrmiensis CFs, estimated to be endangered/critically endangered, and emphasise the need for detailed monitoring and population survey for other cryptic species.
PB  - Basel: MDPI
T2  - Animals
T1  - Cryptic Diversity of the European Blind Mole Rat Nannospalax leucodon Species Complex: Implications for Conservation.
IS  - 9
VL  - 12
DO  - 10.3390/ani12091097
SP  - 1097
ER  - 

@article{
author = "Bugarski-Stanojević, Vanja and Stamenković, Gorana and Jojić, Vida and Ćosić, Nada and Ćirović, Duško and Stojković, Oliver and Veličković, Jelena and Savić, Ivo",
year = "2022",
abstract = "We explored the cryptic speciation of the Nannospalax leucodon species complex, characterised by intense karyotype evolution and reduced phenotypic variability that has produced different lineages, out of which 25 are described as chromosomal forms (CFs), so many cryptic species remain unnoticed. Although some of them should be classified as threatened, they lack the official nomenclature necessary to be involved in conservation strategies. Reproductive isolation between seven CFs has previously been demonstrated. To investigate the amount and dynamics of genetic discrepancy that follows chromosomal changes, infer speciation levels, and obtain phylogenetic patterns, we analysed mitochondrial 16S rRNA and MT-CYTB nucleotide polymorphism among 17 CFs-the highest number studied so far. Phylogenetic trees delineated 11 CFs as separate clades. Evolutionary divergence values overlapped with acknowledged higher taxonomic categories, or sometimes exceeded them. The fact that CFs with higher 2n are evolutionary older corresponds to the fusion hypothesis of Nannospalax karyotype evolution. To participate in conservation strategies, N. leucodon classification should follow the biological species concept, and proposed cryptic species should be formally named, despite a lack of classical morphometric discrepancy. We draw attention towards the syrmiensis and montanosyrmiensis CFs, estimated to be endangered/critically endangered, and emphasise the need for detailed monitoring and population survey for other cryptic species.",
publisher = "Basel: MDPI",
journal = "Animals",
title = "Cryptic Diversity of the European Blind Mole Rat Nannospalax leucodon Species Complex: Implications for Conservation.",
number = "9",
volume = "12",
doi = "10.3390/ani12091097",
pages = "1097"
}

Bugarski-Stanojević, V., Stamenković, G., Jojić, V., Ćosić, N., Ćirović, D., Stojković, O., Veličković, J.,& Savić, I.. (2022). Cryptic Diversity of the European Blind Mole Rat Nannospalax leucodon Species Complex: Implications for Conservation.. in Animals
Basel: MDPI., 12(9), 1097.
https://doi.org/10.3390/ani12091097

Bugarski-Stanojević V, Stamenković G, Jojić V, Ćosić N, Ćirović D, Stojković O, Veličković J, Savić I. Cryptic Diversity of the European Blind Mole Rat Nannospalax leucodon Species Complex: Implications for Conservation.. in Animals. 2022;12(9):1097.
doi:10.3390/ani12091097 .

Bugarski-Stanojević, Vanja, Stamenković, Gorana, Jojić, Vida, Ćosić, Nada, Ćirović, Duško, Stojković, Oliver, Veličković, Jelena, Savić, Ivo, "Cryptic Diversity of the European Blind Mole Rat Nannospalax leucodon Species Complex: Implications for Conservation." in Animals, 12, no. 9 (2022):1097,
https://doi.org/10.3390/ani12091097 . .

TY  - CONF
AU  - Ilić, Marija
AU  - Jovanović, Bogdan
AU  - Stamenković, Gorana
AU  - Bugarski-Stanojević, Vanja
AU  - Paunović, Momir
AU  - Crnobrnja-Isailović, Jelka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5374
AB  - The common frog (Rana temporaria Linnaeus, 1758) is one of the most
widespread and abundant amphibians in Europe, except in the southern parts of the
continent, where distribution is apparently fragmented. It has the greatest genetic
variability of all western Palearctic brown frogs and it is extremely variable in
morphology and ecological preferences, also. The Republic of Serbia is a country of
particular interest in terms of amphibian conservation because of the genetically and
morphologically diverse populations of several amphibian species.
We analysed nucleotide variability of mitochondrial DNA sequences of partial
MT-CYTB gene (461 bp) of 27 specimens of R. temporaria species. Samples (eggs and
tail tips of adult individuals) were collected in two periods, 1986-2007 and 2013-2017,
in 14 localities in Serbia. The analyses revealed nine different haplotypes separated
into two main clusters: north-eastern and eastern (localities Bela Crkva, Grza, Bigar,
Đerdap, Stara planina) and central, west, south-western, and south-eastern Serbia
(localities Jagodnja, Lučani, Zlatibor, Goč, Kopaonik, Prokletije, Šar planina, Oštrozub,
and Vlasina). Lučani and Grza populations had two different haplotypes each.
Our results confirmed previous findings that the common frog shows
phylogeographic substructuring. The existence of two genetically diverged population
groups on the territory of Serbia suggests that these populations should be adequately
protected in terms of the conservation of their breeding places and suitable terrestrial
habitats. In Serbia, R. temporaria habitats are currently not only fragmented but also
under intensive anthropogenic pressure, due to intensive urbanization, particularly in
the mountain areas and the promotion of non-sustainable tourism, which makes them
highly vulnerable. Therefore, there is a need for more precise data about species’
phylogeny and distribution, both at the national and regional level, to define
conservation priorities in the near future
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Phylogeographic substructuring of the common frog (Rana temporaria) in Serbia
SP  - 145
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5374
ER  - 

@conference{
author = "Ilić, Marija and Jovanović, Bogdan and Stamenković, Gorana and Bugarski-Stanojević, Vanja and Paunović, Momir and Crnobrnja-Isailović, Jelka",
year = "2022",
abstract = "The common frog (Rana temporaria Linnaeus, 1758) is one of the most
widespread and abundant amphibians in Europe, except in the southern parts of the
continent, where distribution is apparently fragmented. It has the greatest genetic
variability of all western Palearctic brown frogs and it is extremely variable in
morphology and ecological preferences, also. The Republic of Serbia is a country of
particular interest in terms of amphibian conservation because of the genetically and
morphologically diverse populations of several amphibian species.
We analysed nucleotide variability of mitochondrial DNA sequences of partial
MT-CYTB gene (461 bp) of 27 specimens of R. temporaria species. Samples (eggs and
tail tips of adult individuals) were collected in two periods, 1986-2007 and 2013-2017,
in 14 localities in Serbia. The analyses revealed nine different haplotypes separated
into two main clusters: north-eastern and eastern (localities Bela Crkva, Grza, Bigar,
Đerdap, Stara planina) and central, west, south-western, and south-eastern Serbia
(localities Jagodnja, Lučani, Zlatibor, Goč, Kopaonik, Prokletije, Šar planina, Oštrozub,
and Vlasina). Lučani and Grza populations had two different haplotypes each.
Our results confirmed previous findings that the common frog shows
phylogeographic substructuring. The existence of two genetically diverged population
groups on the territory of Serbia suggests that these populations should be adequately
protected in terms of the conservation of their breeding places and suitable terrestrial
habitats. In Serbia, R. temporaria habitats are currently not only fragmented but also
under intensive anthropogenic pressure, due to intensive urbanization, particularly in
the mountain areas and the promotion of non-sustainable tourism, which makes them
highly vulnerable. Therefore, there is a need for more precise data about species’
phylogeny and distribution, both at the national and regional level, to define
conservation priorities in the near future",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Phylogeographic substructuring of the common frog (Rana temporaria) in Serbia",
pages = "145",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5374"
}

Ilić, M., Jovanović, B., Stamenković, G., Bugarski-Stanojević, V., Paunović, M.,& Crnobrnja-Isailović, J.. (2022). Phylogeographic substructuring of the common frog (Rana temporaria) in Serbia. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 145.
https://hdl.handle.net/21.15107/rcub_ibiss_5374

Ilić M, Jovanović B, Stamenković G, Bugarski-Stanojević V, Paunović M, Crnobrnja-Isailović J. Phylogeographic substructuring of the common frog (Rana temporaria) in Serbia. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:145.
https://hdl.handle.net/21.15107/rcub_ibiss_5374 .

Ilić, Marija, Jovanović, Bogdan, Stamenković, Gorana, Bugarski-Stanojević, Vanja, Paunović, Momir, Crnobrnja-Isailović, Jelka, "Phylogeographic substructuring of the common frog (Rana temporaria) in Serbia" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):145,
https://hdl.handle.net/21.15107/rcub_ibiss_5374 .

TY  - CONF
AU  - Ilić, Marija
AU  - Jovanović, Bogdan
AU  - Stamenković, Gorana
AU  - Bugarski-Stanojević, Vanja
AU  - Zorić, Katarina
AU  - Paunović, Momir
AU  - Crnobrnja-Isailović, Jelka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5373
AB  - Жаба травњача (Rana temporaria Linnaeus, 1758) у Србији насељава претежно планинске и високопланинске пределе. Међутим, због фрагментисаног ареала и нарушених станишта сматра се ретком врстом. Према националној Црвеној Књизи водоземаца, а на основу IUCN категоризације, има статус скоро угрожене врсте (NT – „Near Threatened“), а према Правилнику о проглашењу и заштити строго заштићених и заштићених дивљих биљака, животиња и гљива на територији Србије је строго заштићена врста.
У овом истраживању анализирани су узорци ткива (јаја, врх репа пуноглавца, врх прста адулта) од укупно 28 јединки травњаче са 14 локалитета на територији Србије сакупљених у периоду 1986-2007. и 2013-17. Парцијалне секвенце 16S rRNK гена митохондријалне ДНК (395 бп) показале су постојање шест хаплотипова, сврстаних у две групе које су и географски јасно раздвојене. Првој припадају јединке са локалитета Бела Црква, Бигар, Грза, Ђердап и Стара планина, а другој оне са подручја централне, западне, југозападне и југоисточне Србије (Лучани, Копаоник, Гоч, Јагодња, Проклетије, Златибор, Власина, Оштрозуб, Шар планина). На локалитету Лучани присутна су два различита хаплотипа.
Анализа гена 16S rRNK се показала као адекватна метода за испитивање генетичке и филогеографске структурираности врсте R. temporaria која се налази под интензивним антропогеним притиском. Због угрожавања генофонда, пре свега губитком повољних станшта и променом климе, постоји опасност драстичног смањења дистрибуције ове врсте у Србији, те је неопходно донешење мера у циљу њене заштите и конзервације.
AB  - Žaba travnjača (Rana temporaria Linnaeus, 1758) u Srbiji naseljava pretežno planinske i visokoplaninske predele. Međutim, zbog fragmentisanog areala i narušenih staništa smatra se retkom vrstom. Prema nacionalnoj Crvenoj Knjizi vodozemaca, a na osnovu IUCN kategorizacije, ima status skoro ugrožene vrste (NT – „Near Threatened“), a prema Pravilniku o proglašenju i zaštiti strogo zaštićenih i zaštićenih divljih biljaka, životinja i gljiva na teritoriji Srbije je strogo zaštićena vrsta. U ovom istraživanju analizirani su uzorci tkiva (jaja, vrh repa punoglavca, vrh prsta adulta) od ukupno 28 jedinki travnjače sa 14 lokaliteta na teritoriji Srbije sakupljenih u periodu 1986-2007. i 2013-17. Parcijalne sekvence 16S rRNK gena mitohondrijalne DNK (395 bp) pokazale su postojanje šest haplotipova, svrstanih u dve grupe koje su i geografski jasno razdvojene. Prvoj pripadaju jedinke sa lokaliteta Bela Crkva, Bigar, Grza, Đerdap i Stara planina, a drugoj one sa područja centralne, zapadne, jugozapadne i jugoistočne Srbije (Lučani, Kopaonik, Goč, Jagodnja, Prokletije, Zlatibor, Vlasina, Oštrozub, Šar planina). Na lokalitetu Lučani prisutna su dva različita haplotipa. Analiza gena 16S rRNK se pokazala kao adekvatna metoda za ispitivanje genetičke i filogeografske strukturiranosti vrste R. temporaria koja se nalazi pod intenzivnim antropogenim pritiskom. Zbog ugrožavanja genofonda, pre svega gubitkom povoljnih stanšta i promenom klime, postoji opasnost drastičnog smanjenja distribucije ove vrste u Srbiji, te je neophodno donešenje mera u cilju njene zaštite i konzervacije.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Polimorfizam 16S rRNK gena vrste Rana temporaria na teritoriji Srbije
T1  - Полиморфизам 16S rRNK гена врсте Rana temporaria на територији Србије
SP  - 104
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5373
ER  - 

@conference{
author = "Ilić, Marija and Jovanović, Bogdan and Stamenković, Gorana and Bugarski-Stanojević, Vanja and Zorić, Katarina and Paunović, Momir and Crnobrnja-Isailović, Jelka",
year = "2022",
abstract = "Жаба травњача (Rana temporaria Linnaeus, 1758) у Србији насељава претежно планинске и високопланинске пределе. Међутим, због фрагментисаног ареала и нарушених станишта сматра се ретком врстом. Према националној Црвеној Књизи водоземаца, а на основу IUCN категоризације, има статус скоро угрожене врсте (NT – „Near Threatened“), а према Правилнику о проглашењу и заштити строго заштићених и заштићених дивљих биљака, животиња и гљива на територији Србије је строго заштићена врста.
У овом истраживању анализирани су узорци ткива (јаја, врх репа пуноглавца, врх прста адулта) од укупно 28 јединки травњаче са 14 локалитета на територији Србије сакупљених у периоду 1986-2007. и 2013-17. Парцијалне секвенце 16S rRNK гена митохондријалне ДНК (395 бп) показале су постојање шест хаплотипова, сврстаних у две групе које су и географски јасно раздвојене. Првој припадају јединке са локалитета Бела Црква, Бигар, Грза, Ђердап и Стара планина, а другој оне са подручја централне, западне, југозападне и југоисточне Србије (Лучани, Копаоник, Гоч, Јагодња, Проклетије, Златибор, Власина, Оштрозуб, Шар планина). На локалитету Лучани присутна су два различита хаплотипа.
Анализа гена 16S rRNK се показала као адекватна метода за испитивање генетичке и филогеографске структурираности врсте R. temporaria која се налази под интензивним антропогеним притиском. Због угрожавања генофонда, пре свега губитком повољних станшта и променом климе, постоји опасност драстичног смањења дистрибуције ове врсте у Србији, те је неопходно донешење мера у циљу њене заштите и конзервације., Žaba travnjača (Rana temporaria Linnaeus, 1758) u Srbiji naseljava pretežno planinske i visokoplaninske predele. Međutim, zbog fragmentisanog areala i narušenih staništa smatra se retkom vrstom. Prema nacionalnoj Crvenoj Knjizi vodozemaca, a na osnovu IUCN kategorizacije, ima status skoro ugrožene vrste (NT – „Near Threatened“), a prema Pravilniku o proglašenju i zaštiti strogo zaštićenih i zaštićenih divljih biljaka, životinja i gljiva na teritoriji Srbije je strogo zaštićena vrsta. U ovom istraživanju analizirani su uzorci tkiva (jaja, vrh repa punoglavca, vrh prsta adulta) od ukupno 28 jedinki travnjače sa 14 lokaliteta na teritoriji Srbije sakupljenih u periodu 1986-2007. i 2013-17. Parcijalne sekvence 16S rRNK gena mitohondrijalne DNK (395 bp) pokazale su postojanje šest haplotipova, svrstanih u dve grupe koje su i geografski jasno razdvojene. Prvoj pripadaju jedinke sa lokaliteta Bela Crkva, Bigar, Grza, Đerdap i Stara planina, a drugoj one sa područja centralne, zapadne, jugozapadne i jugoistočne Srbije (Lučani, Kopaonik, Goč, Jagodnja, Prokletije, Zlatibor, Vlasina, Oštrozub, Šar planina). Na lokalitetu Lučani prisutna su dva različita haplotipa. Analiza gena 16S rRNK se pokazala kao adekvatna metoda za ispitivanje genetičke i filogeografske strukturiranosti vrste R. temporaria koja se nalazi pod intenzivnim antropogenim pritiskom. Zbog ugrožavanja genofonda, pre svega gubitkom povoljnih stanšta i promenom klime, postoji opasnost drastičnog smanjenja distribucije ove vrste u Srbiji, te je neophodno donešenje mera u cilju njene zaštite i konzervacije.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Polimorfizam 16S rRNK gena vrste Rana temporaria na teritoriji Srbije, Полиморфизам 16S rRNK гена врсте Rana temporaria на територији Србије",
pages = "104",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5373"
}

Ilić, M., Jovanović, B., Stamenković, G., Bugarski-Stanojević, V., Zorić, K., Paunović, M.,& Crnobrnja-Isailović, J.. (2022). Polimorfizam 16S rRNK gena vrste Rana temporaria na teritoriji Srbije. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 104.
https://hdl.handle.net/21.15107/rcub_ibiss_5373

Ilić M, Jovanović B, Stamenković G, Bugarski-Stanojević V, Zorić K, Paunović M, Crnobrnja-Isailović J. Polimorfizam 16S rRNK gena vrste Rana temporaria na teritoriji Srbije. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:104.
https://hdl.handle.net/21.15107/rcub_ibiss_5373 .

Ilić, Marija, Jovanović, Bogdan, Stamenković, Gorana, Bugarski-Stanojević, Vanja, Zorić, Katarina, Paunović, Momir, Crnobrnja-Isailović, Jelka, "Polimorfizam 16S rRNK gena vrste Rana temporaria na teritoriji Srbije" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):104,
https://hdl.handle.net/21.15107/rcub_ibiss_5373 .

TY  - CONF
AU  - Đokić, Marko
AU  - Stamenković, Gorana
AU  - Jojić, Vida
AU  - Ćosić, Nada
AU  - Ćirović, Duško
AU  - Savić, Ivo
AU  - Bugarski-Stanojević, Vanja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5312
AB  - Vrste slepog kučeta iz roda Nannospalax se, pored brojnih adaptacija na podzemni način života, karakterišu i izraženom varijabilnošću kariotipa. Do sada su opisane čak 74 različite hromozomske forme (HF), ali su zvanično priznate samo tri vrste.1 Svaka je zapravo kompleks vrsta sa statusom „nedovoljno podataka“ na IUCN Red List of Threatened Species. Evropske vrste ovog roda sačinjavaju kompleks N. leucodon supersp.1 Među njima je više od 20 različitih HF, od kojih je pet (hungaricus, serbicus, montanoserbicus, syrmiensis, montanosyrmiensis) prisutno u Srbiji, a poslednje dve se vode kao ugrožene.2 Iako su međusobno reproduktivno izolovane i filogenetski udaljene, zbog nedostatka morfoloških razlika predstavljaju kriptične vrste. Sa konzervacionog aspekta je veoma važno vršiti monitoring njihovih populacija i utvrditi areale rasprostanjenja. Stoga je neophodno razviti pouzdanu i neinvazivnu metodu za njihovu efikasnu determinaciju. Inter Simple Sequence Repeat ˗ Polymerase Chain Reaction (ISSR-PCR) tehnika, koja produkuje ISSR markere – anonimne sekvence DNK ograničene invertovanim tandemskim ponovcima, se pokazala kao korisna u identifikaciji morfološki sličnih vrsta kod nekoliko sisarskih taksona.3 Od 11 različitih ISSR prajmera testiranih na prisustvo fragmenata DNK specifičnih za vrstu, na ukupnom uzorku od 25 jedinki, tri prajmera (CAA)5GC; (CAG)4AC i (GA)8AC su dala informativne profile DNK, obezbeđujući pouzdano i jasno prepoznavanje svih pet analiziranih kriptičnih vrsta.
AB  - Врсте слепог кучета из рода Nannospalax се, поред бројних адаптација на подземни начин живота, карактеришу и израженом варијабилношћу кариотипа. До сада су описане чак 74 различите хромозомске форме (ХФ), али су званично признате само три врсте.1 Свака је заправо комплекс врста са статусом „недовољно података“ на IUCN Red List of Threatened Species. Европске врсте овог рода сачињавају комплекс N. leucodon supersp.1 Међу њима је више од 20 различитих ХФ, од којих је пет (hungaricus, serbicus, montanoserbicus, syrmiensis, montanosyrmiensis) присутно у Србији, а последње две се воде као угрожене.2 Иако су међусобно репродуктивно изоловане и филогенетски удаљене, због недостатка морфолошких разлика представљају криптичне врсте. Са конзервационог аспекта је веома важно вршити мониторинг њихових популација и утврдити ареале распростањења. Стога је неопходно развити поуздану и неинвазивну методу за њихову ефикасну детерминацију. Inter Simple Sequence Repeat ˗ Polymerase Chain Reaction (ISSR-PCR) техника, која продукује ISSR маркере – анонимне секвенце ДНК ограничене инвертованим тандемским поновцима, се показала као корисна у идентификацији морфолошки сличних врста код неколико сисарских таксона.3 Од 11 различитих ISSR прајмера тестираних на присуство фрагмената ДНК специфичних за врсту, на укупном узорку од 25 јединки, три прајмера (CAA)5GC; (CAG)4AC и (GA)8AC су дала информативне профиле ДНК, обезбеђујући поуздано и јасно препознавање свих пет анализираних криптичних врста.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - ISSR-PCR kao jednostavna dijagnostička metoda za razlikovanje kriptičnih vrsta Nannospalax leucodon (Spalacinae, Rodentia)
T1  - ISSR-PCR као једноставна дијагностичка метода за разликовање криптичних врста Nannospalax leucodon (Spalacinae, Rodentia)
SP  - 106
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5312
ER  - 

@conference{
author = "Đokić, Marko and Stamenković, Gorana and Jojić, Vida and Ćosić, Nada and Ćirović, Duško and Savić, Ivo and Bugarski-Stanojević, Vanja",
year = "2022",
abstract = "Vrste slepog kučeta iz roda Nannospalax se, pored brojnih adaptacija na podzemni način života, karakterišu i izraženom varijabilnošću kariotipa. Do sada su opisane čak 74 različite hromozomske forme (HF), ali su zvanično priznate samo tri vrste.1 Svaka je zapravo kompleks vrsta sa statusom „nedovoljno podataka“ na IUCN Red List of Threatened Species. Evropske vrste ovog roda sačinjavaju kompleks N. leucodon supersp.1 Među njima je više od 20 različitih HF, od kojih je pet (hungaricus, serbicus, montanoserbicus, syrmiensis, montanosyrmiensis) prisutno u Srbiji, a poslednje dve se vode kao ugrožene.2 Iako su međusobno reproduktivno izolovane i filogenetski udaljene, zbog nedostatka morfoloških razlika predstavljaju kriptične vrste. Sa konzervacionog aspekta je veoma važno vršiti monitoring njihovih populacija i utvrditi areale rasprostanjenja. Stoga je neophodno razviti pouzdanu i neinvazivnu metodu za njihovu efikasnu determinaciju. Inter Simple Sequence Repeat ˗ Polymerase Chain Reaction (ISSR-PCR) tehnika, koja produkuje ISSR markere – anonimne sekvence DNK ograničene invertovanim tandemskim ponovcima, se pokazala kao korisna u identifikaciji morfološki sličnih vrsta kod nekoliko sisarskih taksona.3 Od 11 različitih ISSR prajmera testiranih na prisustvo fragmenata DNK specifičnih za vrstu, na ukupnom uzorku od 25 jedinki, tri prajmera (CAA)5GC; (CAG)4AC i (GA)8AC su dala informativne profile DNK, obezbeđujući pouzdano i jasno prepoznavanje svih pet analiziranih kriptičnih vrsta., Врсте слепог кучета из рода Nannospalax се, поред бројних адаптација на подземни начин живота, карактеришу и израженом варијабилношћу кариотипа. До сада су описане чак 74 различите хромозомске форме (ХФ), али су званично признате само три врсте.1 Свака је заправо комплекс врста са статусом „недовољно података“ на IUCN Red List of Threatened Species. Европске врсте овог рода сачињавају комплекс N. leucodon supersp.1 Међу њима је више од 20 различитих ХФ, од којих је пет (hungaricus, serbicus, montanoserbicus, syrmiensis, montanosyrmiensis) присутно у Србији, а последње две се воде као угрожене.2 Иако су међусобно репродуктивно изоловане и филогенетски удаљене, због недостатка морфолошких разлика представљају криптичне врсте. Са конзервационог аспекта је веома важно вршити мониторинг њихових популација и утврдити ареале распростањења. Стога је неопходно развити поуздану и неинвазивну методу за њихову ефикасну детерминацију. Inter Simple Sequence Repeat ˗ Polymerase Chain Reaction (ISSR-PCR) техника, која продукује ISSR маркере – анонимне секвенце ДНК ограничене инвертованим тандемским поновцима, се показала као корисна у идентификацији морфолошки сличних врста код неколико сисарских таксона.3 Од 11 различитих ISSR прајмера тестираних на присуство фрагмената ДНК специфичних за врсту, на укупном узорку од 25 јединки, три прајмера (CAA)5GC; (CAG)4AC и (GA)8AC су дала информативне профиле ДНК, обезбеђујући поуздано и јасно препознавање свих пет анализираних криптичних врста.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "ISSR-PCR kao jednostavna dijagnostička metoda za razlikovanje kriptičnih vrsta Nannospalax leucodon (Spalacinae, Rodentia), ISSR-PCR као једноставна дијагностичка метода за разликовање криптичних врста Nannospalax leucodon (Spalacinae, Rodentia)",
pages = "106",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5312"
}

Đokić, M., Stamenković, G., Jojić, V., Ćosić, N., Ćirović, D., Savić, I.,& Bugarski-Stanojević, V.. (2022). ISSR-PCR kao jednostavna dijagnostička metoda za razlikovanje kriptičnih vrsta Nannospalax leucodon (Spalacinae, Rodentia). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 106.
https://hdl.handle.net/21.15107/rcub_ibiss_5312

Đokić M, Stamenković G, Jojić V, Ćosić N, Ćirović D, Savić I, Bugarski-Stanojević V. ISSR-PCR kao jednostavna dijagnostička metoda za razlikovanje kriptičnih vrsta Nannospalax leucodon (Spalacinae, Rodentia). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:106.
https://hdl.handle.net/21.15107/rcub_ibiss_5312 .

Đokić, Marko, Stamenković, Gorana, Jojić, Vida, Ćosić, Nada, Ćirović, Duško, Savić, Ivo, Bugarski-Stanojević, Vanja, "ISSR-PCR kao jednostavna dijagnostička metoda za razlikovanje kriptičnih vrsta Nannospalax leucodon (Spalacinae, Rodentia)" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):106,
https://hdl.handle.net/21.15107/rcub_ibiss_5312 .

TY  - JOUR
AU  - Bugarski-Stanojević, Vanja
AU  - Stamenković, Gorana
AU  - Ćirović, Duško
AU  - Ćirić, Danica
AU  - Stojković, Oliver
AU  - Veličković, Jelena
AU  - Kataranovski, Dragan
AU  - Savić, Ivo
PY  - 2020
UR  - https://link.springer.com/article/10.1007/s42991-020-00019-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3660
AB  - The role of intraspecific karyotype variability in reproductive isolation and speciation has been widely studied. Among the 26 genera of Palaearctic mammals, the blind mole rats genus Nannospalax has the highest karyotype variability with 74 chromosomal forms (CFs). Although these CFs have been described in detail, taxonomic effects of chromosomal rearrangements are still lacking, especially among 25 recorded CFs of European N. leucodon superspecies. As genetic discrepancies for most of them are missing, we analyze nucleotide sequence polymorphism of the mitochondrial 16S rRNA gene between eight N. leucodon CFs. Here we provide for the first time nucleotide sequence data for three CFs: monticola, montanoserbicus and syrmiensis using 40–57-year-old archived samples from our mammalian collection and thus demonstrate the usefulness of archived/museum samples as starting material for DNA analysis. The topology of the phylogenetic tree is congruent with the traditional taxonomic separation of recent blind mole rats with high support. Diversification of N. leucodon cluster into discrete subclusters—CFs—and the extent of evolutionary divergence among them are in accordance with previous findings of complete reproductive isolation between six CFs analyzed here. Additionally, the level of evolutionary divergence among six N. leucodon CFs resembles those recorded among clearly distinct Spalax species and four proposed species of N. ehrenbergi. These facts suggest that they could be cryptic species and bring attention to their conservation and natural resource protection.
PB  - Springer
T2  - Mammalian Biology
T1  - 16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae)
VL  - 100
DO  - 10.1007/s42991-020-00019-9
SP  - 315
EP  - 324
ER  - 

@article{
author = "Bugarski-Stanojević, Vanja and Stamenković, Gorana and Ćirović, Duško and Ćirić, Danica and Stojković, Oliver and Veličković, Jelena and Kataranovski, Dragan and Savić, Ivo",
year = "2020",
abstract = "The role of intraspecific karyotype variability in reproductive isolation and speciation has been widely studied. Among the 26 genera of Palaearctic mammals, the blind mole rats genus Nannospalax has the highest karyotype variability with 74 chromosomal forms (CFs). Although these CFs have been described in detail, taxonomic effects of chromosomal rearrangements are still lacking, especially among 25 recorded CFs of European N. leucodon superspecies. As genetic discrepancies for most of them are missing, we analyze nucleotide sequence polymorphism of the mitochondrial 16S rRNA gene between eight N. leucodon CFs. Here we provide for the first time nucleotide sequence data for three CFs: monticola, montanoserbicus and syrmiensis using 40–57-year-old archived samples from our mammalian collection and thus demonstrate the usefulness of archived/museum samples as starting material for DNA analysis. The topology of the phylogenetic tree is congruent with the traditional taxonomic separation of recent blind mole rats with high support. Diversification of N. leucodon cluster into discrete subclusters—CFs—and the extent of evolutionary divergence among them are in accordance with previous findings of complete reproductive isolation between six CFs analyzed here. Additionally, the level of evolutionary divergence among six N. leucodon CFs resembles those recorded among clearly distinct Spalax species and four proposed species of N. ehrenbergi. These facts suggest that they could be cryptic species and bring attention to their conservation and natural resource protection.",
publisher = "Springer",
journal = "Mammalian Biology",
title = "16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae)",
volume = "100",
doi = "10.1007/s42991-020-00019-9",
pages = "315-324"
}

Bugarski-Stanojević, V., Stamenković, G., Ćirović, D., Ćirić, D., Stojković, O., Veličković, J., Kataranovski, D.,& Savić, I.. (2020). 16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae). in Mammalian Biology
Springer., 100, 315-324.
https://doi.org/10.1007/s42991-020-00019-9

Bugarski-Stanojević V, Stamenković G, Ćirović D, Ćirić D, Stojković O, Veličković J, Kataranovski D, Savić I. 16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae). in Mammalian Biology. 2020;100:315-324.
doi:10.1007/s42991-020-00019-9 .

Bugarski-Stanojević, Vanja, Stamenković, Gorana, Ćirović, Duško, Ćirić, Danica, Stojković, Oliver, Veličković, Jelena, Kataranovski, Dragan, Savić, Ivo, "16S rRNA gene polymorphism supports cryptic speciation within the lesser blind mole rat Nannospalax leucodon superspecies (Rodentia: Spalacidae)" in Mammalian Biology, 100 (2020):315-324,
https://doi.org/10.1007/s42991-020-00019-9 . .

TY  - CONF
AU  - Bugarski-Stanojević, Vanja
AU  - Stamenković, Gorana
AU  - Ćirović, Duško
AU  - Ćirić, Danica
AU  - Stojković, Oliver
AU  - Veličković, Jelena
AU  - Kataranovski, Dragan
AU  - Savić, Ivo
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5657
AB  - Among 26 genera of Palaearctic mammals, the genus Nannospalax has the highest karyotype
variability with 74 chromosomal forms (CFs). Taxonomic effects i.e. implications to phylogeny
and speciation process of such chromosomal variety are still lacking, especially among 25
reported CFs of South-European N. leucodon superspecies. Many cryptic species are under
serious threat of complete disappearance, with population declines in Europe. As genetic
discrepancies for the majority of them are missing, we analyzed nucleotide sequence
polymorphism of the mitochondrial 16S rRNA gene between eight N. leucodon CFs and also
add for the first time nucleotide sequence data for three CFs: monticola, montanoserbicus
and syrmiensis. Further, including 40-57 years old teeth we evaluate the usefulness of the
archived samples, e.g. from museums and other old collections as starting material for
phylogenetic analysis. The topology of the Bayesian Inference tree is in agreement with the
traditional taxonomic separation of recent blind mole rats. Among the three superspecies, the
genetic diversity was lowest in N. ehrenbergi (0.004-0.031), highest in N. xanthodon (0.009-
0.063) and intermediate in N. leucodon (0.008-0.055). The comparable scale of evolutionary
divergence among N. leucodon CFs and among species from the genus Spalax supports our
previous proposal that seven reproductively isolated CFs should be considered to be cryptic
species and thus protected from extinction in their natural habitat.
PB  - Belgrade: Serbian Genetic Society
C3  - 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
T1  - 16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae)
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5657
ER  - 

@conference{
author = "Bugarski-Stanojević, Vanja and Stamenković, Gorana and Ćirović, Duško and Ćirić, Danica and Stojković, Oliver and Veličković, Jelena and Kataranovski, Dragan and Savić, Ivo",
year = "2019",
abstract = "Among 26 genera of Palaearctic mammals, the genus Nannospalax has the highest karyotype
variability with 74 chromosomal forms (CFs). Taxonomic effects i.e. implications to phylogeny
and speciation process of such chromosomal variety are still lacking, especially among 25
reported CFs of South-European N. leucodon superspecies. Many cryptic species are under
serious threat of complete disappearance, with population declines in Europe. As genetic
discrepancies for the majority of them are missing, we analyzed nucleotide sequence
polymorphism of the mitochondrial 16S rRNA gene between eight N. leucodon CFs and also
add for the first time nucleotide sequence data for three CFs: monticola, montanoserbicus
and syrmiensis. Further, including 40-57 years old teeth we evaluate the usefulness of the
archived samples, e.g. from museums and other old collections as starting material for
phylogenetic analysis. The topology of the Bayesian Inference tree is in agreement with the
traditional taxonomic separation of recent blind mole rats. Among the three superspecies, the
genetic diversity was lowest in N. ehrenbergi (0.004-0.031), highest in N. xanthodon (0.009-
0.063) and intermediate in N. leucodon (0.008-0.055). The comparable scale of evolutionary
divergence among N. leucodon CFs and among species from the genus Spalax supports our
previous proposal that seven reproductively isolated CFs should be considered to be cryptic
species and thus protected from extinction in their natural habitat.",
publisher = "Belgrade: Serbian Genetic Society",
journal = "6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia",
title = "16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae)",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5657"
}

Bugarski-Stanojević, V., Stamenković, G., Ćirović, D., Ćirić, D., Stojković, O., Veličković, J., Kataranovski, D.,& Savić, I.. (2019). 16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae). in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
Belgrade: Serbian Genetic Society..
https://hdl.handle.net/21.15107/rcub_ibiss_5657

Bugarski-Stanojević V, Stamenković G, Ćirović D, Ćirić D, Stojković O, Veličković J, Kataranovski D, Savić I. 16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae). in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia. 2019;.
https://hdl.handle.net/21.15107/rcub_ibiss_5657 .

Bugarski-Stanojević, Vanja, Stamenković, Gorana, Ćirović, Duško, Ćirić, Danica, Stojković, Oliver, Veličković, Jelena, Kataranovski, Dragan, Savić, Ivo, "16s rRNA gene polymorphism supports cryptic speciation within the Lesser Blind Mole Rat Nannospalax leucodon Superspecies (Rodentia: Spalacidae)" in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia (2019),
https://hdl.handle.net/21.15107/rcub_ibiss_5657 .

TY  - JOUR
AU  - Gajić, Bojan
AU  - Bugarski-Stanojević, Vanja
AU  - Penezić, Aleksandra
AU  - Kuručki, Milica
AU  - Bogdanović, Neda
AU  - Ćirović, Duško
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4796
AB  - Thelazia callipaeda, originally known as an “Oriental eyeworm,” is a small nematode parasitizing the conjunctival sacs of
domestic and wild animals and humans. Previous studies conducted in Serbia have reported the eyeworm infections in dogs,
cats, and foxes, as well as in a human patient. As the data regarding thelaziosis from wildlife is still scarce, the aim of this study
was to investigate the presence of T. callipaeda in gray wolf (Canis lupus) from Serbia. All collected nematodes were morphologically
identified as T. callipaeda males (n = 64) or females (n = 225). Molecular characterization, conducted by PCR
amplification followed by sequence analysis of partial cytochrome c oxidase subunit 1 gene (cox1), revealed only haplotype 1
of T. callipaeda. The overall prevalence of thelaziosis was 38.1% (8/21). In all positive animals, both eyes were affected, with a
total parasitic load ranging from four to 132 worms per animal. Our results indicate the important epidemiological role of wolves
as wildlife reservoirs of T. callipaeda, expanding geographic range of infection, as well as intra- and interspecies contact rates,
although the role of other wild carnivore species (i.e., foxes and jackals) should be investigated in future studies.
PB  - New York: Springer Nature
T2  - Parasitology Research
T1  - First report of eyeworm infection by Thelazia callipaeda in gray wolf (Canis lupus) from Serbia
VL  - 118
DO  - 10.1007/s00436-019-06519-z
SP  - 3549
EP  - 3553
ER  - 

@article{
author = "Gajić, Bojan and Bugarski-Stanojević, Vanja and Penezić, Aleksandra and Kuručki, Milica and Bogdanović, Neda and Ćirović, Duško",
year = "2019",
abstract = "Thelazia callipaeda, originally known as an “Oriental eyeworm,” is a small nematode parasitizing the conjunctival sacs of
domestic and wild animals and humans. Previous studies conducted in Serbia have reported the eyeworm infections in dogs,
cats, and foxes, as well as in a human patient. As the data regarding thelaziosis from wildlife is still scarce, the aim of this study
was to investigate the presence of T. callipaeda in gray wolf (Canis lupus) from Serbia. All collected nematodes were morphologically
identified as T. callipaeda males (n = 64) or females (n = 225). Molecular characterization, conducted by PCR
amplification followed by sequence analysis of partial cytochrome c oxidase subunit 1 gene (cox1), revealed only haplotype 1
of T. callipaeda. The overall prevalence of thelaziosis was 38.1% (8/21). In all positive animals, both eyes were affected, with a
total parasitic load ranging from four to 132 worms per animal. Our results indicate the important epidemiological role of wolves
as wildlife reservoirs of T. callipaeda, expanding geographic range of infection, as well as intra- and interspecies contact rates,
although the role of other wild carnivore species (i.e., foxes and jackals) should be investigated in future studies.",
publisher = "New York: Springer Nature",
journal = "Parasitology Research",
title = "First report of eyeworm infection by Thelazia callipaeda in gray wolf (Canis lupus) from Serbia",
volume = "118",
doi = "10.1007/s00436-019-06519-z",
pages = "3549-3553"
}

Gajić, B., Bugarski-Stanojević, V., Penezić, A., Kuručki, M., Bogdanović, N.,& Ćirović, D.. (2019). First report of eyeworm infection by Thelazia callipaeda in gray wolf (Canis lupus) from Serbia. in Parasitology Research
New York: Springer Nature., 118, 3549-3553.
https://doi.org/10.1007/s00436-019-06519-z

Gajić B, Bugarski-Stanojević V, Penezić A, Kuručki M, Bogdanović N, Ćirović D. First report of eyeworm infection by Thelazia callipaeda in gray wolf (Canis lupus) from Serbia. in Parasitology Research. 2019;118:3549-3553.
doi:10.1007/s00436-019-06519-z .

Gajić, Bojan, Bugarski-Stanojević, Vanja, Penezić, Aleksandra, Kuručki, Milica, Bogdanović, Neda, Ćirović, Duško, "First report of eyeworm infection by Thelazia callipaeda in gray wolf (Canis lupus) from Serbia" in Parasitology Research, 118 (2019):3549-3553,
https://doi.org/10.1007/s00436-019-06519-z . .

TY  - JOUR
AU  - Savić, Ivo
AU  - Ćirović, Duško
AU  - Bugarski-Stanojević, Vanja
PY  - 2017
UR  - http://www.mdpi.com/2073-4425/8/11/292
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2895
AB  - Mole rats are exclusively subterranean and highly specialized rodents. Their long lifespans, remarkable anti-cancer mechanisms, and various distinctive adaptive features make them a useful research model. Moreover, opposing convergence of morphological traits, they have developed extremely high karyotype variability. Thus, 74 chromosomal forms have been described so far and new ones are being revealed continuously. These evolved during the process of rapid radiation and occur in different biogeographical regions. During research into their reproductive biology we have already provided substantial evidence for species-level separation of these taxa. Here, we review diverse chromosomal forms of the lesser blind mole rat, Mediterranean Nannospalax leucodon, distributed in South-eastern Europe, their karyotype records, biogeography, origin, and phylogeny from our extensive research. In the light of new data from molecular genetic studies, we question some former valuations and propose a cryptospecies rank for seven reproductively isolated chromosomal forms with sympatric and parapatric distribution and clear ecogeographical discrepances in their habitats, as well as new experimental and theoretical methods for understanding the courses of speciation of these unique fossorial mammals.
T2  - Genes
T1  - Exceptional Chromosomal Evolution and Cryptic Speciation of Blind Mole Rats Nannospalax leucodon (Spalacinae, Rodentia) from South-Eastern Europe
IS  - 11
VL  - 8
DO  - 10.3390/genes8110292
SP  - 292
EP  - 292
ER  - 

@article{
author = "Savić, Ivo and Ćirović, Duško and Bugarski-Stanojević, Vanja",
year = "2017",
abstract = "Mole rats are exclusively subterranean and highly specialized rodents. Their long lifespans, remarkable anti-cancer mechanisms, and various distinctive adaptive features make them a useful research model. Moreover, opposing convergence of morphological traits, they have developed extremely high karyotype variability. Thus, 74 chromosomal forms have been described so far and new ones are being revealed continuously. These evolved during the process of rapid radiation and occur in different biogeographical regions. During research into their reproductive biology we have already provided substantial evidence for species-level separation of these taxa. Here, we review diverse chromosomal forms of the lesser blind mole rat, Mediterranean Nannospalax leucodon, distributed in South-eastern Europe, their karyotype records, biogeography, origin, and phylogeny from our extensive research. In the light of new data from molecular genetic studies, we question some former valuations and propose a cryptospecies rank for seven reproductively isolated chromosomal forms with sympatric and parapatric distribution and clear ecogeographical discrepances in their habitats, as well as new experimental and theoretical methods for understanding the courses of speciation of these unique fossorial mammals.",
journal = "Genes",
title = "Exceptional Chromosomal Evolution and Cryptic Speciation of Blind Mole Rats Nannospalax leucodon (Spalacinae, Rodentia) from South-Eastern Europe",
number = "11",
volume = "8",
doi = "10.3390/genes8110292",
pages = "292-292"
}

Savić, I., Ćirović, D.,& Bugarski-Stanojević, V.. (2017). Exceptional Chromosomal Evolution and Cryptic Speciation of Blind Mole Rats Nannospalax leucodon (Spalacinae, Rodentia) from South-Eastern Europe. in Genes, 8(11), 292-292.
https://doi.org/10.3390/genes8110292

Savić I, Ćirović D, Bugarski-Stanojević V. Exceptional Chromosomal Evolution and Cryptic Speciation of Blind Mole Rats Nannospalax leucodon (Spalacinae, Rodentia) from South-Eastern Europe. in Genes. 2017;8(11):292-292.
doi:10.3390/genes8110292 .

Savić, Ivo, Ćirović, Duško, Bugarski-Stanojević, Vanja, "Exceptional Chromosomal Evolution and Cryptic Speciation of Blind Mole Rats Nannospalax leucodon (Spalacinae, Rodentia) from South-Eastern Europe" in Genes, 8, no. 11 (2017):292-292,
https://doi.org/10.3390/genes8110292 . .

TY  - JOUR
AU  - Bugarski-Stanojević, Vanja
AU  - Stamenković, Gorana
AU  - Blagojević, Jelena
AU  - Liehr, Thomas
AU  - Kosyakova, Nadezda
AU  - Rajičić, Marija
AU  - Vujošević, Mladen
PY  - 2016
UR  - http://dx.plos.org/10.1371/journal.pone.0160946
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2524
AB  - Since the density of simple sequence repeats (SSRs) may vary between different chromosomes of the same species in eukaryotic genomes, we screened SSRs of the whole genome of the yellow necked mouse, Apodemus flavicollis, in order to reveal SSR profiles specific for animals carrying B chromosomes. We found that the 2200 bp band was amplified by primer (CAG)4AC to a highly increased level in samples with B chromosomes. This quantitative difference (B-marker) between animals with (+B) and without (0B) B chromosomes was used to screen 20 populations (387 animals). The presence/absence of Bs was confirmed in 96.5% of 342 non mosaic individuals, which recommends this method for noninvasive B-presence detection. A group of 45 animals with mosaic and micro B (μB) karyotypes was considered separately and showed 55.6% of overall congruence between karyotyping and molecular screening results. Relative quantification by qPCR of two different targeted sequences from B-marker indicated that these B-specific fragments are multiplied on B chromosomes. It also confirms our assumption that different types of Bs with variable molecular composition may exist in the same individual and between individuals of this species. Our results substantiate the origin of Bs from the standard chromosomal complement. The B-marker showed 98% sequence identity with the serine/threonine protein kinase VRK1 gene, similarly to findings reported for Bs from phylogenetically highly distant mammalian species. Evolutionarily conserved protein-coding genes found in Bs, including this one in A. flavicollis, could suggest a common evolutionary pathway.
PB  - Public Library of Science
T2  - PLOS ONE
T1  - Exploring Supernumeraries - A New Marker for Screening of B-Chromosomes Presence in the Yellow Necked Mouse Apodemus flavicollis
IS  - 8
VL  - 11
DO  - 10.1371/journal.pone.0160946
SP  - e0160946
EP  - e0160946
ER  - 

@article{
author = "Bugarski-Stanojević, Vanja and Stamenković, Gorana and Blagojević, Jelena and Liehr, Thomas and Kosyakova, Nadezda and Rajičić, Marija and Vujošević, Mladen",
year = "2016",
abstract = "Since the density of simple sequence repeats (SSRs) may vary between different chromosomes of the same species in eukaryotic genomes, we screened SSRs of the whole genome of the yellow necked mouse, Apodemus flavicollis, in order to reveal SSR profiles specific for animals carrying B chromosomes. We found that the 2200 bp band was amplified by primer (CAG)4AC to a highly increased level in samples with B chromosomes. This quantitative difference (B-marker) between animals with (+B) and without (0B) B chromosomes was used to screen 20 populations (387 animals). The presence/absence of Bs was confirmed in 96.5% of 342 non mosaic individuals, which recommends this method for noninvasive B-presence detection. A group of 45 animals with mosaic and micro B (μB) karyotypes was considered separately and showed 55.6% of overall congruence between karyotyping and molecular screening results. Relative quantification by qPCR of two different targeted sequences from B-marker indicated that these B-specific fragments are multiplied on B chromosomes. It also confirms our assumption that different types of Bs with variable molecular composition may exist in the same individual and between individuals of this species. Our results substantiate the origin of Bs from the standard chromosomal complement. The B-marker showed 98% sequence identity with the serine/threonine protein kinase VRK1 gene, similarly to findings reported for Bs from phylogenetically highly distant mammalian species. Evolutionarily conserved protein-coding genes found in Bs, including this one in A. flavicollis, could suggest a common evolutionary pathway.",
publisher = "Public Library of Science",
journal = "PLOS ONE",
title = "Exploring Supernumeraries - A New Marker for Screening of B-Chromosomes Presence in the Yellow Necked Mouse Apodemus flavicollis",
number = "8",
volume = "11",
doi = "10.1371/journal.pone.0160946",
pages = "e0160946-e0160946"
}

Bugarski-Stanojević, V., Stamenković, G., Blagojević, J., Liehr, T., Kosyakova, N., Rajičić, M.,& Vujošević, M.. (2016). Exploring Supernumeraries - A New Marker for Screening of B-Chromosomes Presence in the Yellow Necked Mouse Apodemus flavicollis. in PLOS ONE
Public Library of Science., 11(8), e0160946-e0160946.
https://doi.org/10.1371/journal.pone.0160946

Bugarski-Stanojević V, Stamenković G, Blagojević J, Liehr T, Kosyakova N, Rajičić M, Vujošević M. Exploring Supernumeraries - A New Marker for Screening of B-Chromosomes Presence in the Yellow Necked Mouse Apodemus flavicollis. in PLOS ONE. 2016;11(8):e0160946-e0160946.
doi:10.1371/journal.pone.0160946 .

Bugarski-Stanojević, Vanja, Stamenković, Gorana, Blagojević, Jelena, Liehr, Thomas, Kosyakova, Nadezda, Rajičić, Marija, Vujošević, Mladen, "Exploring Supernumeraries - A New Marker for Screening of B-Chromosomes Presence in the Yellow Necked Mouse Apodemus flavicollis" in PLOS ONE, 11, no. 8 (2016):e0160946-e0160946,
https://doi.org/10.1371/journal.pone.0160946 . .

TY  - CONF
AU  - Rajičić, Marija
AU  - Adnađević, Tanja
AU  - Bugarski-Stanojević, Vanja
AU  - Stamenković, Gorana
AU  - Jojić, Vida
AU  - Budinski, Ivana
AU  - Jovanović, Vladimir
AU  - Blagojević, Jelena
AU  - Vujošević, Mladen
PY  - 2015
UR  - http://chromosome2015.mcb.nsc.ru/chr_2015_abstracts.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5423
AB  - In genus Apodemus, the presence of B chromosome has been confirmed in six out of
twenty-two species. One of them is yellow-necked field mouse Apodemus flavicollis. The
presence of B chromosomes has been confirmed in almost all studied populations of A. flavicollis
46
in Serbia in a wide range of frequencies (0,07- 0,63). Five B chromosomes per animal was the
highest number of Bs recorded in our analyzed populations.
These extra chromosomes in A. flavicollis are euchromatic and acrocentric, and by size
and distribution of G- and C-bands can be sorted into the group of five smallest chromosomes in
karyotype.
The absence of Bs accumulation in male meiosis, no significant difference in the mean
number of scars and embryos between females with and without Bs, as well as the absence of
significant difference in the presence of Bs among six age categories, speaks in favor of heterotic
model of their maintenance, contrary to the model of parasitic behavior of these additional
genomic elements.
In general, the number of Bs carriers is higher in environment that is not optimal for this
species which indicates that those individuals have higher fitness. Seasonal variation of Bs
frequencies has been recorded, in such a manner that highest frequencies are present before and
after winter, with stable frequency fluctuations during successive years. Different frequency of Bs
carriers is related to adaptive differentiation to diverse habitats mediated by environmental
variables that directly and/or indirectly influence population dynamics of A. flavicollis. Changes
in frequency follow up increase in population density in circumstances without food and space
competition. Positive correlation between the frequency of Bs carriers and the average number of
sub zero days, as well as with altitude increase, has been recorded.
Considering phenotypic effects, higher level of morphological integration of mandible in
animals with Bs in A. flavicollis has been found. Furthermore, studies of the effects of Bs on
nonmetric cranial traits, as well as on three components of cranial variability confirmed that Bs
does not disturb developmental homeostasis in their carriers. Moreover, Bs play a significant role
in structuring cranial variation.
Negative correlation between the expression of Tgf-β gene and the presence of Bs was also
observed. This could be of great importance for B chromosome which has to pass through
different mitotic and meiotic check points, and Tgf-β gene, through cell cycle regulation,
influences development and homeostasis.
The existence of specific DNA profiles and differential expressions of three genes in the
presence of Bs, testify about interaction of Bs with the rest of the genome. Absence of C-KIT
exon 1 in more than one copy in specimens with 1, 2 and 3B chromosomes excludes the
possibility that this gene is present on Bs. On the other hand, presence of S18 gene copy has been
indicated on some Bs.
Considering the results of population studies, euchromatic structure of Bs and molecular
studies that have been conducted so far, we can say that the contribution of Bs to overall genetic
47
diversity in this species is in sight, but there is still need for more specific evidence such as
precise determination of Bs molecular structure, origin and function.
PB  - Institute of molecular and cellular biology SB RAS
C3  - Abstracts of the International conference “Chromosome 2015”; 2015 Aug 24-28; Novosibirsk, Russia
T1  - B chromosomes in Apodemus flavicollis
SP  - 45
EP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5423
ER  - 

@conference{
author = "Rajičić, Marija and Adnađević, Tanja and Bugarski-Stanojević, Vanja and Stamenković, Gorana and Jojić, Vida and Budinski, Ivana and Jovanović, Vladimir and Blagojević, Jelena and Vujošević, Mladen",
year = "2015",
abstract = "In genus Apodemus, the presence of B chromosome has been confirmed in six out of
twenty-two species. One of them is yellow-necked field mouse Apodemus flavicollis. The
presence of B chromosomes has been confirmed in almost all studied populations of A. flavicollis
46
in Serbia in a wide range of frequencies (0,07- 0,63). Five B chromosomes per animal was the
highest number of Bs recorded in our analyzed populations.
These extra chromosomes in A. flavicollis are euchromatic and acrocentric, and by size
and distribution of G- and C-bands can be sorted into the group of five smallest chromosomes in
karyotype.
The absence of Bs accumulation in male meiosis, no significant difference in the mean
number of scars and embryos between females with and without Bs, as well as the absence of
significant difference in the presence of Bs among six age categories, speaks in favor of heterotic
model of their maintenance, contrary to the model of parasitic behavior of these additional
genomic elements.
In general, the number of Bs carriers is higher in environment that is not optimal for this
species which indicates that those individuals have higher fitness. Seasonal variation of Bs
frequencies has been recorded, in such a manner that highest frequencies are present before and
after winter, with stable frequency fluctuations during successive years. Different frequency of Bs
carriers is related to adaptive differentiation to diverse habitats mediated by environmental
variables that directly and/or indirectly influence population dynamics of A. flavicollis. Changes
in frequency follow up increase in population density in circumstances without food and space
competition. Positive correlation between the frequency of Bs carriers and the average number of
sub zero days, as well as with altitude increase, has been recorded.
Considering phenotypic effects, higher level of morphological integration of mandible in
animals with Bs in A. flavicollis has been found. Furthermore, studies of the effects of Bs on
nonmetric cranial traits, as well as on three components of cranial variability confirmed that Bs
does not disturb developmental homeostasis in their carriers. Moreover, Bs play a significant role
in structuring cranial variation.
Negative correlation between the expression of Tgf-β gene and the presence of Bs was also
observed. This could be of great importance for B chromosome which has to pass through
different mitotic and meiotic check points, and Tgf-β gene, through cell cycle regulation,
influences development and homeostasis.
The existence of specific DNA profiles and differential expressions of three genes in the
presence of Bs, testify about interaction of Bs with the rest of the genome. Absence of C-KIT
exon 1 in more than one copy in specimens with 1, 2 and 3B chromosomes excludes the
possibility that this gene is present on Bs. On the other hand, presence of S18 gene copy has been
indicated on some Bs.
Considering the results of population studies, euchromatic structure of Bs and molecular
studies that have been conducted so far, we can say that the contribution of Bs to overall genetic
47
diversity in this species is in sight, but there is still need for more specific evidence such as
precise determination of Bs molecular structure, origin and function.",
publisher = "Institute of molecular and cellular biology SB RAS",
journal = "Abstracts of the International conference “Chromosome 2015”; 2015 Aug 24-28; Novosibirsk, Russia",
title = "B chromosomes in Apodemus flavicollis",
pages = "45-47",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5423"
}

Rajičić, M., Adnađević, T., Bugarski-Stanojević, V., Stamenković, G., Jojić, V., Budinski, I., Jovanović, V., Blagojević, J.,& Vujošević, M.. (2015). B chromosomes in Apodemus flavicollis. in Abstracts of the International conference “Chromosome 2015”; 2015 Aug 24-28; Novosibirsk, Russia
Institute of molecular and cellular biology SB RAS., 45-47.
https://hdl.handle.net/21.15107/rcub_ibiss_5423

Rajičić M, Adnađević T, Bugarski-Stanojević V, Stamenković G, Jojić V, Budinski I, Jovanović V, Blagojević J, Vujošević M. B chromosomes in Apodemus flavicollis. in Abstracts of the International conference “Chromosome 2015”; 2015 Aug 24-28; Novosibirsk, Russia. 2015;:45-47.
https://hdl.handle.net/21.15107/rcub_ibiss_5423 .

Rajičić, Marija, Adnađević, Tanja, Bugarski-Stanojević, Vanja, Stamenković, Gorana, Jojić, Vida, Budinski, Ivana, Jovanović, Vladimir, Blagojević, Jelena, Vujošević, Mladen, "B chromosomes in Apodemus flavicollis" in Abstracts of the International conference “Chromosome 2015”; 2015 Aug 24-28; Novosibirsk, Russia (2015):45-47,
https://hdl.handle.net/21.15107/rcub_ibiss_5423 .

TY  - JOUR
AU  - Stamenković, Gorana
AU  - Nikolic, V.
AU  - Blagojević, Jelena
AU  - Bugarski-Stanojević, Vanja
AU  - Adnađević, Tanja
AU  - Stanojevic, M.
AU  - Vujošević, Mladen
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1999
AB  - Dobrava-Belgrade virus (DOBV) is a hantavirus species that causes the
   most severe form of haemorrhagic fever with renal syndrome (HFRS) in
   Europe. DOBV has been detected in three Apodemus rodents: A.flavicollis,
   A.agrarius and A.ponticus. These emerging viruses appear throughout the
   Balkan Peninsula including Serbia as its central part. In this study, we
   examined the seroprevalence, molecular epidemiology and phylogenetics of
   DOBV from A.flavicollis captured at six Serbian localities. Furthermore,
   we applied microsatellite typing of host animal genome to analyse the
   role of host kinship in DOBV animal transmission. The overall IgG
   seropositivity rate over 3years (2008-2010) was 11.9\% (22/185). All
   seropositive samples were subjected to RT-PCR and DNA sequencing for S
   and L genome segments (pos. 291-1079nt and 2999-3316nt, respectively).
   DOBV was genetically detected in three samples from mountain Tara in
   western Serbia, a newly detected DOBV focus in the Balkans. No sequence
   data from human cases from Serbia are available for the studied period.
   However, collected DOBV isolates in this work phylogenetically clustered
   together with isolates from Serbian human cases dating from 2002, with
   1.9\% nucleotide divergence. We determined the level of kinship between
   seropositive and seronegative animal groups and found no significant
   difference, suggesting that horizontal virus transmission in the studied
   population was the same within and among the hatches. Our findings are
   the first genetic detection of DOBV in rodents in Serbia. We confirm
   wide and continuous hantavirus presence in the examined parts of the
   Balkans, underlying the necessity of continual monitoring of hantavirus
   circulation in A.flavicollis.
T2  - Zoonoses and Public Health
T1  - Genetic Analysis of Dobrava-Belgrade Virus from Western Serbia - A Newly
 Detected Focus in the Balkan Peninsula
IS  - 2
VL  - 62
DO  - 10.1111/zph.12136
SP  - 141
EP  - 150
ER  - 

@article{
author = "Stamenković, Gorana and Nikolic, V. and Blagojević, Jelena and Bugarski-Stanojević, Vanja and Adnađević, Tanja and Stanojevic, M. and Vujošević, Mladen",
year = "2015",
abstract = "Dobrava-Belgrade virus (DOBV) is a hantavirus species that causes the
   most severe form of haemorrhagic fever with renal syndrome (HFRS) in
   Europe. DOBV has been detected in three Apodemus rodents: A.flavicollis,
   A.agrarius and A.ponticus. These emerging viruses appear throughout the
   Balkan Peninsula including Serbia as its central part. In this study, we
   examined the seroprevalence, molecular epidemiology and phylogenetics of
   DOBV from A.flavicollis captured at six Serbian localities. Furthermore,
   we applied microsatellite typing of host animal genome to analyse the
   role of host kinship in DOBV animal transmission. The overall IgG
   seropositivity rate over 3years (2008-2010) was 11.9\% (22/185). All
   seropositive samples were subjected to RT-PCR and DNA sequencing for S
   and L genome segments (pos. 291-1079nt and 2999-3316nt, respectively).
   DOBV was genetically detected in three samples from mountain Tara in
   western Serbia, a newly detected DOBV focus in the Balkans. No sequence
   data from human cases from Serbia are available for the studied period.
   However, collected DOBV isolates in this work phylogenetically clustered
   together with isolates from Serbian human cases dating from 2002, with
   1.9\% nucleotide divergence. We determined the level of kinship between
   seropositive and seronegative animal groups and found no significant
   difference, suggesting that horizontal virus transmission in the studied
   population was the same within and among the hatches. Our findings are
   the first genetic detection of DOBV in rodents in Serbia. We confirm
   wide and continuous hantavirus presence in the examined parts of the
   Balkans, underlying the necessity of continual monitoring of hantavirus
   circulation in A.flavicollis.",
journal = "Zoonoses and Public Health",
title = "Genetic Analysis of Dobrava-Belgrade Virus from Western Serbia - A Newly
 Detected Focus in the Balkan Peninsula",
number = "2",
volume = "62",
doi = "10.1111/zph.12136",
pages = "141-150"
}

Stamenković, G., Nikolic, V., Blagojević, J., Bugarski-Stanojević, V., Adnađević, T., Stanojevic, M.,& Vujošević, M.. (2015). Genetic Analysis of Dobrava-Belgrade Virus from Western Serbia - A Newly
 Detected Focus in the Balkan Peninsula. in Zoonoses and Public Health, 62(2), 141-150.
https://doi.org/10.1111/zph.12136

Stamenković G, Nikolic V, Blagojević J, Bugarski-Stanojević V, Adnađević T, Stanojevic M, Vujošević M. Genetic Analysis of Dobrava-Belgrade Virus from Western Serbia - A Newly
 Detected Focus in the Balkan Peninsula. in Zoonoses and Public Health. 2015;62(2):141-150.
doi:10.1111/zph.12136 .

Stamenković, Gorana, Nikolic, V., Blagojević, Jelena, Bugarski-Stanojević, Vanja, Adnađević, Tanja, Stanojevic, M., Vujošević, Mladen, "Genetic Analysis of Dobrava-Belgrade Virus from Western Serbia - A Newly
 Detected Focus in the Balkan Peninsula" in Zoonoses and Public Health, 62, no. 2 (2015):141-150,
https://doi.org/10.1111/zph.12136 . .

TY  - JOUR
AU  - Jojić, Vida
AU  - Bugarski-Stanojević, Vanja
AU  - Blagojević, Jelena
AU  - Vujošević, Mladen
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2285
AB  - Karyotyping and several molecular methods have allowed successful
   identification of two morphologically similar wide-ranging Western
   Palearctic species, the yellow-necked field mouse Apodemus flavicollis
   (Melchior, 1934) and the long-tailed wood mouse A. sylvaticus (Linnaeus,
   1758), but reliable species diagnosis on the basis of morphometric
   characters is particularly problematic. Although they are easily
   morphologically distinguishable in Central and Northern Europe, this is
   not the case in southern parts of their distribution areas. Despite
   that, we have successfully discriminated A. flavicollis and A.
   sylvaticus from Serbia (Southern Europe) using geometric and traditional
   morphometric methods on a data set for ventral crania of specimens
   previously genotyped by the Inter Simple Sequence Repeat-PCR (ISSR-PCR).
   Discrimination power of applied approaches was more or less similar. The
   majority of our results were consistent with those obtained for
   specimens collected across the Czech Republic (Central Europe).
   Morphological differences observed herein, as well as those already
   reported between A. flavicollis and A. sylvaticus from the central and
   northern parts of their distribution areas, could be the outcome of
   their biology, i.e. ecological discrepancies, different assumed
   evolutionary scenarios considering biogeography, phylogeny, history and
   ontogeny. (C) 2014 Elsevier GmbH. All rights reserved.
T2  - Zoologischer Anzeiger
T1  - Discrimination of the sibling species Apodemus flavicollis and
 A-sylvaticus (Rodentia, Muridae)
IS  - 4
VL  - 253
DO  - 10.1016/j.jcz.2014.02.002
SP  - 261
EP  - 269
ER  - 

@article{
author = "Jojić, Vida and Bugarski-Stanojević, Vanja and Blagojević, Jelena and Vujošević, Mladen",
year = "2014",
abstract = "Karyotyping and several molecular methods have allowed successful
   identification of two morphologically similar wide-ranging Western
   Palearctic species, the yellow-necked field mouse Apodemus flavicollis
   (Melchior, 1934) and the long-tailed wood mouse A. sylvaticus (Linnaeus,
   1758), but reliable species diagnosis on the basis of morphometric
   characters is particularly problematic. Although they are easily
   morphologically distinguishable in Central and Northern Europe, this is
   not the case in southern parts of their distribution areas. Despite
   that, we have successfully discriminated A. flavicollis and A.
   sylvaticus from Serbia (Southern Europe) using geometric and traditional
   morphometric methods on a data set for ventral crania of specimens
   previously genotyped by the Inter Simple Sequence Repeat-PCR (ISSR-PCR).
   Discrimination power of applied approaches was more or less similar. The
   majority of our results were consistent with those obtained for
   specimens collected across the Czech Republic (Central Europe).
   Morphological differences observed herein, as well as those already
   reported between A. flavicollis and A. sylvaticus from the central and
   northern parts of their distribution areas, could be the outcome of
   their biology, i.e. ecological discrepancies, different assumed
   evolutionary scenarios considering biogeography, phylogeny, history and
   ontogeny. (C) 2014 Elsevier GmbH. All rights reserved.",
journal = "Zoologischer Anzeiger",
title = "Discrimination of the sibling species Apodemus flavicollis and
 A-sylvaticus (Rodentia, Muridae)",
number = "4",
volume = "253",
doi = "10.1016/j.jcz.2014.02.002",
pages = "261-269"
}

Jojić, V., Bugarski-Stanojević, V., Blagojević, J.,& Vujošević, M.. (2014). Discrimination of the sibling species Apodemus flavicollis and
 A-sylvaticus (Rodentia, Muridae). in Zoologischer Anzeiger, 253(4), 261-269.
https://doi.org/10.1016/j.jcz.2014.02.002

Jojić V, Bugarski-Stanojević V, Blagojević J, Vujošević M. Discrimination of the sibling species Apodemus flavicollis and
 A-sylvaticus (Rodentia, Muridae). in Zoologischer Anzeiger. 2014;253(4):261-269.
doi:10.1016/j.jcz.2014.02.002 .

Jojić, Vida, Bugarski-Stanojević, Vanja, Blagojević, Jelena, Vujošević, Mladen, "Discrimination of the sibling species Apodemus flavicollis and
 A-sylvaticus (Rodentia, Muridae)" in Zoologischer Anzeiger, 253, no. 4 (2014):261-269,
https://doi.org/10.1016/j.jcz.2014.02.002 . .

TY  - JOUR
AU  - Bugarski-Stanojević, Vanja
AU  - Blagojević, Jelena
AU  - Adnađević, Tanja
AU  - Jovanović, Vladimir
AU  - Vujošević, Mladen
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1053
AB  - The yellow-necked field mouse, Apodemus flavicollis (Melchior, 1934), and the long-tailed field mouse, Apodemus sylvaticus (Linnaeus, 1758) are morphologically similar species, with largely overlapping geographical areas and almost equal ecological requirements. This makes reliable species diagnosis based on external characters a real challenge. When advanced multivariate methods of skull and/or dental morphometrics are employed, specimens can be successfully distinguished in most cases, but not all, making single specimen identification impossible. Application of C-band karyotyping clearly distinguishes between these two species. However, it can be applied only to live animals. Several molecular methods have also allowed successful species diagnosis, but their universality has not been tested. Here we compare the diagnostic power of three molecular approaches and their applicability to populations from a species wide distributional area, as well as their simplicity of use, both for live animals and for samples stored in alcohol for a considerable period, without DNA sequencing. A total of 200 tissue samples from Morocco, France, Belgium, Serbia, Romania, Greece, Russia, Turkey and Kazakhstan, were analyzed by AP-PCR, ISSR-PCR and species-specific primers from the mitochondrial cytochrome b gene. The first two methods gave clear species-specific DNA profiles in complete agreement with previous C-band results. However, only partial agreement was observed between the third, species-specific primer method and the other two approaches and C-banding, as there were 4.5-10.5% false positive results. Hence we propose AP-PCR and ISSR-PCR techniques with particular chosen primers for quick and reliable diagnosis of these two species. A large number of samples can be assayed in a short period, with minimal cost and effort. Moreover, these techniques can be applied to a single specimen. (C) 2012 Elsevier GmbH. All rights reserved.
T2  - Zoologischer Anzeiger
T1  - Identification of the sibling species Apodemus sylvaticus and Apodemus flavicollis (Rodentia, Muridae)-Comparison of molecular methods
IS  - 4
VL  - 252
DO  - 10.1016/j.jcz.2012.11.004
SP  - 329
EP  - 587
ER  - 

@article{
author = "Bugarski-Stanojević, Vanja and Blagojević, Jelena and Adnađević, Tanja and Jovanović, Vladimir and Vujošević, Mladen",
year = "2013",
abstract = "The yellow-necked field mouse, Apodemus flavicollis (Melchior, 1934), and the long-tailed field mouse, Apodemus sylvaticus (Linnaeus, 1758) are morphologically similar species, with largely overlapping geographical areas and almost equal ecological requirements. This makes reliable species diagnosis based on external characters a real challenge. When advanced multivariate methods of skull and/or dental morphometrics are employed, specimens can be successfully distinguished in most cases, but not all, making single specimen identification impossible. Application of C-band karyotyping clearly distinguishes between these two species. However, it can be applied only to live animals. Several molecular methods have also allowed successful species diagnosis, but their universality has not been tested. Here we compare the diagnostic power of three molecular approaches and their applicability to populations from a species wide distributional area, as well as their simplicity of use, both for live animals and for samples stored in alcohol for a considerable period, without DNA sequencing. A total of 200 tissue samples from Morocco, France, Belgium, Serbia, Romania, Greece, Russia, Turkey and Kazakhstan, were analyzed by AP-PCR, ISSR-PCR and species-specific primers from the mitochondrial cytochrome b gene. The first two methods gave clear species-specific DNA profiles in complete agreement with previous C-band results. However, only partial agreement was observed between the third, species-specific primer method and the other two approaches and C-banding, as there were 4.5-10.5% false positive results. Hence we propose AP-PCR and ISSR-PCR techniques with particular chosen primers for quick and reliable diagnosis of these two species. A large number of samples can be assayed in a short period, with minimal cost and effort. Moreover, these techniques can be applied to a single specimen. (C) 2012 Elsevier GmbH. All rights reserved.",
journal = "Zoologischer Anzeiger",
title = "Identification of the sibling species Apodemus sylvaticus and Apodemus flavicollis (Rodentia, Muridae)-Comparison of molecular methods",
number = "4",
volume = "252",
doi = "10.1016/j.jcz.2012.11.004",
pages = "329-587"
}

Bugarski-Stanojević, V., Blagojević, J., Adnađević, T., Jovanović, V.,& Vujošević, M.. (2013). Identification of the sibling species Apodemus sylvaticus and Apodemus flavicollis (Rodentia, Muridae)-Comparison of molecular methods. in Zoologischer Anzeiger, 252(4), 329-587.
https://doi.org/10.1016/j.jcz.2012.11.004

Bugarski-Stanojević V, Blagojević J, Adnađević T, Jovanović V, Vujošević M. Identification of the sibling species Apodemus sylvaticus and Apodemus flavicollis (Rodentia, Muridae)-Comparison of molecular methods. in Zoologischer Anzeiger. 2013;252(4):329-587.
doi:10.1016/j.jcz.2012.11.004 .

Bugarski-Stanojević, Vanja, Blagojević, Jelena, Adnađević, Tanja, Jovanović, Vladimir, Vujošević, Mladen, "Identification of the sibling species Apodemus sylvaticus and Apodemus flavicollis (Rodentia, Muridae)-Comparison of molecular methods" in Zoologischer Anzeiger, 252, no. 4 (2013):329-587,
https://doi.org/10.1016/j.jcz.2012.11.004 . .

TY  - JOUR
AU  - Adnađević, Tanja
AU  - Stamenković, Gorana
AU  - Bugarski-Stanojević, Vanja
AU  - Blagojević, Jelena
AU  - Vujošević, Mladen
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1105
AB  - Two alternative models are used to explain maintenance of polymorphism of B chromosomes (Bs) in populations of a great number of species. The parasitic model suggests deleterious effects of Bs on fitness of carriers, while the heterotic model assumes that, in the absence of drive, equilibrium is produced by beneficial effects of Bs at low numbers. In order to determine the potential contribution of Bs to genetic differentiation and diversity, four populations of Apodemus flavicollis, differing in frequency of Bs (from 0.23 to 0.38) and settled in ecologically different habitats, were analyzed by 471 AFLP markers. Although numerous loci were demonstrated to be population specific, none of them was associated with individuals with Bs. AMOVA showed that the presence of Bs does not affect population differentiation, pointing to greater genetic similarity of Bs to A chromosomes. The greatest genetic diversity (0.241) was found in the population settled in optimal conditions for this species featured by the lowest frequency of animals with Bs (0.23). We found that the majority of loci marked as loci under directional selection, are characteristic of either a population with lower or one with a higher frequency of Bs. Several loci detected as outliers were associated with environmental variables that could directly and/or indirectly influence population dynamics of A. flavicollis. Thus, we suggest that the different frequency of Bs carriers in populations is related to adaptive differentiation to diverse habitats, which is in accordance with the heterotic model of Bs maintenance.
T2  - Population Ecology
T1  - Genetic differentiation in populations of the yellow-necked mouse, Apodemus flavicollis, harbouring B chromosomes in different frequencies
IS  - 4
VL  - 54
DO  - 10.1007/s10144-012-0333-6
SP  - 7
EP  - 548
ER  - 

@article{
author = "Adnađević, Tanja and Stamenković, Gorana and Bugarski-Stanojević, Vanja and Blagojević, Jelena and Vujošević, Mladen",
year = "2012",
abstract = "Two alternative models are used to explain maintenance of polymorphism of B chromosomes (Bs) in populations of a great number of species. The parasitic model suggests deleterious effects of Bs on fitness of carriers, while the heterotic model assumes that, in the absence of drive, equilibrium is produced by beneficial effects of Bs at low numbers. In order to determine the potential contribution of Bs to genetic differentiation and diversity, four populations of Apodemus flavicollis, differing in frequency of Bs (from 0.23 to 0.38) and settled in ecologically different habitats, were analyzed by 471 AFLP markers. Although numerous loci were demonstrated to be population specific, none of them was associated with individuals with Bs. AMOVA showed that the presence of Bs does not affect population differentiation, pointing to greater genetic similarity of Bs to A chromosomes. The greatest genetic diversity (0.241) was found in the population settled in optimal conditions for this species featured by the lowest frequency of animals with Bs (0.23). We found that the majority of loci marked as loci under directional selection, are characteristic of either a population with lower or one with a higher frequency of Bs. Several loci detected as outliers were associated with environmental variables that could directly and/or indirectly influence population dynamics of A. flavicollis. Thus, we suggest that the different frequency of Bs carriers in populations is related to adaptive differentiation to diverse habitats, which is in accordance with the heterotic model of Bs maintenance.",
journal = "Population Ecology",
title = "Genetic differentiation in populations of the yellow-necked mouse, Apodemus flavicollis, harbouring B chromosomes in different frequencies",
number = "4",
volume = "54",
doi = "10.1007/s10144-012-0333-6",
pages = "7-548"
}

Adnađević, T., Stamenković, G., Bugarski-Stanojević, V., Blagojević, J.,& Vujošević, M.. (2012). Genetic differentiation in populations of the yellow-necked mouse, Apodemus flavicollis, harbouring B chromosomes in different frequencies. in Population Ecology, 54(4), 7-548.
https://doi.org/10.1007/s10144-012-0333-6

Adnađević T, Stamenković G, Bugarski-Stanojević V, Blagojević J, Vujošević M. Genetic differentiation in populations of the yellow-necked mouse, Apodemus flavicollis, harbouring B chromosomes in different frequencies. in Population Ecology. 2012;54(4):7-548.
doi:10.1007/s10144-012-0333-6 .

Adnađević, Tanja, Stamenković, Gorana, Bugarski-Stanojević, Vanja, Blagojević, Jelena, Vujošević, Mladen, "Genetic differentiation in populations of the yellow-necked mouse, Apodemus flavicollis, harbouring B chromosomes in different frequencies" in Population Ecology, 54, no. 4 (2012):7-548,
https://doi.org/10.1007/s10144-012-0333-6 . .

TY  - JOUR
AU  - Blagojević, Jelena
AU  - Jovanović, Vladimir
AU  - Stamenković, Gorana
AU  - Jojić, Vida
AU  - Bugarski-Stanojević, Vanja
AU  - Adnađević, Tanja
AU  - Vujošević, Mladen
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4648
AB  - Bioaccumulation of heavy metals in the skulls of black-striped field mice (Apodemusagrarius)
was compared for two localities in Serbia differing in the level of pollution. Eight heavy metals: Fe, Mn, Co,
Cd, Zn, Ni, Pb and Cu, were quantitatively analyzed by atomic absorption spectrophotometry.Four of them
(Mn, Cd, Fe and Ni) showed significantly higher concentrations at the polluted location than in the relatively
unpolluted one. Concentrations of heavy metals in three age categories exhibited opposite patterns depending
on pollution levels. At the unpolluted locality heavy metal concentrations were the highest in the youngest
group and lower in older animals. In contrast, bone metal concentrations increased with age class at the more
polluted site. At the same time, we found that young animals from the polluted location had a statistically
significant lower relative body mass (RBM) than those from the unpolluted area(t = 7.155, p < 0.001), which
may have been caused by heavy metals or other factors associated with pollution. In general, we found that age
is a critical factor for estimating the level of heavy metal pollution, so proper investigations should account for
the age structure of the samples.
PB  - Tehran: University of Tehran, Graduate Faculty of Environment
T2  - International Journal of Environmental Research
T1  - Age Differences in Bioaccumulation of Heavy Metals in Populations of the Black-Striped Field Mouse, Apodemusagrarius(Rodentia, Mammalia)
IS  - 4
VL  - 6
DO  - 10.22059/ijer.2012.575
SP  - 1045
EP  - 1052
ER  - 

@article{
author = "Blagojević, Jelena and Jovanović, Vladimir and Stamenković, Gorana and Jojić, Vida and Bugarski-Stanojević, Vanja and Adnađević, Tanja and Vujošević, Mladen",
year = "2012",
abstract = "Bioaccumulation of heavy metals in the skulls of black-striped field mice (Apodemusagrarius)
was compared for two localities in Serbia differing in the level of pollution. Eight heavy metals: Fe, Mn, Co,
Cd, Zn, Ni, Pb and Cu, were quantitatively analyzed by atomic absorption spectrophotometry.Four of them
(Mn, Cd, Fe and Ni) showed significantly higher concentrations at the polluted location than in the relatively
unpolluted one. Concentrations of heavy metals in three age categories exhibited opposite patterns depending
on pollution levels. At the unpolluted locality heavy metal concentrations were the highest in the youngest
group and lower in older animals. In contrast, bone metal concentrations increased with age class at the more
polluted site. At the same time, we found that young animals from the polluted location had a statistically
significant lower relative body mass (RBM) than those from the unpolluted area(t = 7.155, p < 0.001), which
may have been caused by heavy metals or other factors associated with pollution. In general, we found that age
is a critical factor for estimating the level of heavy metal pollution, so proper investigations should account for
the age structure of the samples.",
publisher = "Tehran: University of Tehran, Graduate Faculty of Environment",
journal = "International Journal of Environmental Research",
title = "Age Differences in Bioaccumulation of Heavy Metals in Populations of the Black-Striped Field Mouse, Apodemusagrarius(Rodentia, Mammalia)",
number = "4",
volume = "6",
doi = "10.22059/ijer.2012.575",
pages = "1045-1052"
}

Blagojević, J., Jovanović, V., Stamenković, G., Jojić, V., Bugarski-Stanojević, V., Adnađević, T.,& Vujošević, M.. (2012). Age Differences in Bioaccumulation of Heavy Metals in Populations of the Black-Striped Field Mouse, Apodemusagrarius(Rodentia, Mammalia). in International Journal of Environmental Research
Tehran: University of Tehran, Graduate Faculty of Environment., 6(4), 1045-1052.
https://doi.org/10.22059/ijer.2012.575

Blagojević J, Jovanović V, Stamenković G, Jojić V, Bugarski-Stanojević V, Adnađević T, Vujošević M. Age Differences in Bioaccumulation of Heavy Metals in Populations of the Black-Striped Field Mouse, Apodemusagrarius(Rodentia, Mammalia). in International Journal of Environmental Research. 2012;6(4):1045-1052.
doi:10.22059/ijer.2012.575 .

Blagojević, Jelena, Jovanović, Vladimir, Stamenković, Gorana, Jojić, Vida, Bugarski-Stanojević, Vanja, Adnađević, Tanja, Vujošević, Mladen, "Age Differences in Bioaccumulation of Heavy Metals in Populations of the Black-Striped Field Mouse, Apodemusagrarius(Rodentia, Mammalia)" in International Journal of Environmental Research, 6, no. 4 (2012):1045-1052,
https://doi.org/10.22059/ijer.2012.575 . .

TY  - JOUR
AU  - Bugarski-Stanojević, Vanja
AU  - Blagojević, Jelena
AU  - Stamenković, Gorana
AU  - Adnađević, Tanja
AU  - Giagia-Athanasopoulou, Eva B
AU  - Vujošević, Mladen
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1316
AB  - The utility of the Inter Simple Sequence Repeat-Polymerase Chain Reaction (ISSR-PCR) was explored in order to determine genetic variation in six species of the genus Apodemus (A. flavicollis, A. sylvaticus, A. uralensis, A. agrarius, A. mystacinus and A. epimelas) at the individual level, population level, in separate geographic samples and in the species as a whole. Six optimized primers produced highly reproducible and polymorphic DNA markers with 98.3% polymorphic bands on a total sample of 91 individuals from 32 localities in Europe and Asia. Moreover, each primer allowed for an exact diagnosis of each of the six Apodemus species and thus provides a simple and reliable tool for the hitherto problematic discrimination of species from the subgenus Sylvaemus. Genetic distances between species ranged from 0.079, among the closely related A. flavicollis and A. sylvaticus, to 0.203 between A. mystacinus and A. agrarius. A. flavicollis, A. uralensis and A. sylvaticus display a strong population substructure. The range of genetic distances among geographic samples within last two species reaches the values obtained for closely related species. ISSR markers proved to be a simple and reliable tool for species diagnosis, as well as for estimating genetic diversity below the species level and for closely related species, but they showed questionable reliability for larger genetic distances.
T2  - Systematics and Biodiversity
T1  - Comparative study of the phylogenetic structure in six Apodemus species (Mammalia, Rodentia) inferred from ISSR-PCR data
IS  - 1
VL  - 9
DO  - 10.1080/14772000.2011.560970
EP  - 106
ER  - 

@article{
author = "Bugarski-Stanojević, Vanja and Blagojević, Jelena and Stamenković, Gorana and Adnađević, Tanja and Giagia-Athanasopoulou, Eva B and Vujošević, Mladen",
year = "2011",
abstract = "The utility of the Inter Simple Sequence Repeat-Polymerase Chain Reaction (ISSR-PCR) was explored in order to determine genetic variation in six species of the genus Apodemus (A. flavicollis, A. sylvaticus, A. uralensis, A. agrarius, A. mystacinus and A. epimelas) at the individual level, population level, in separate geographic samples and in the species as a whole. Six optimized primers produced highly reproducible and polymorphic DNA markers with 98.3% polymorphic bands on a total sample of 91 individuals from 32 localities in Europe and Asia. Moreover, each primer allowed for an exact diagnosis of each of the six Apodemus species and thus provides a simple and reliable tool for the hitherto problematic discrimination of species from the subgenus Sylvaemus. Genetic distances between species ranged from 0.079, among the closely related A. flavicollis and A. sylvaticus, to 0.203 between A. mystacinus and A. agrarius. A. flavicollis, A. uralensis and A. sylvaticus display a strong population substructure. The range of genetic distances among geographic samples within last two species reaches the values obtained for closely related species. ISSR markers proved to be a simple and reliable tool for species diagnosis, as well as for estimating genetic diversity below the species level and for closely related species, but they showed questionable reliability for larger genetic distances.",
journal = "Systematics and Biodiversity",
title = "Comparative study of the phylogenetic structure in six Apodemus species (Mammalia, Rodentia) inferred from ISSR-PCR data",
number = "1",
volume = "9",
doi = "10.1080/14772000.2011.560970",
pages = "106"
}

Bugarski-Stanojević, V., Blagojević, J., Stamenković, G., Adnađević, T., Giagia-Athanasopoulou, E. B.,& Vujošević, M.. (2011). Comparative study of the phylogenetic structure in six Apodemus species (Mammalia, Rodentia) inferred from ISSR-PCR data. in Systematics and Biodiversity, 9(1).
https://doi.org/10.1080/14772000.2011.560970

Bugarski-Stanojević V, Blagojević J, Stamenković G, Adnađević T, Giagia-Athanasopoulou EB, Vujošević M. Comparative study of the phylogenetic structure in six Apodemus species (Mammalia, Rodentia) inferred from ISSR-PCR data. in Systematics and Biodiversity. 2011;9(1):null-106.
doi:10.1080/14772000.2011.560970 .

Bugarski-Stanojević, Vanja, Blagojević, Jelena, Stamenković, Gorana, Adnađević, Tanja, Giagia-Athanasopoulou, Eva B, Vujošević, Mladen, "Comparative study of the phylogenetic structure in six Apodemus species (Mammalia, Rodentia) inferred from ISSR-PCR data" in Systematics and Biodiversity, 9, no. 1 (2011),
https://doi.org/10.1080/14772000.2011.560970 . .

TY  - JOUR
AU  - Vujošević, Mladen
AU  - Blagojević, Jelena
AU  - Jojić, Vida
AU  - Bugarski-Stanojević, Vanja
AU  - Adnađević, Tanja
AU  - Stamenković, Gorana
PY  - 2009
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/236
AB  - The presence of B chromosomes (Bs) is frequently found in populations of yellow-necked mouse, Apodemus flavicollis. Differences in frequencies of Bs in age categories were analyzed in 717 animals in order to clarify the mechanisms of their maintenance in populations of A. flavicollis. The absence of statistically significant differences in the frequency of Bs between six age categories indicates that the maintenance of Bs in populations can be explained by in terms of their contribution to overall genetic diversity of the species rather than by parasitic behavior.
AB  - Prekobrojni hromozomi nazivaju se B hromozomi ako zadovoljavaju tri kriterijuma: nisu neophodni za preživljavanje, ne sparuju se sa hromozomima A seta i ne nasleđuju se mendelovski. B hromozomi su vrlo često prisutni u populacijama žutogrlog miša, Apodemus flavicollis. Njihovo održavanje u populacijama objašnjava se sa dva suprostavljena modela. Model označen kao parazitski tvrdi da se B hromozomi održavaju zahvaljujući mehanizmu akumulacije uprkos štetnim efektima na fitnes nosilaca. S druge strane, heterotički model sugeriše da u odsustvu akumulacije mali broj B hromozoma ima adaptivnu prednost za nosioce, dok veći broj može biti štetan. Ako B hromozomi, koji su označeni kao parazitski, redukuju fitnes kod jedinki koje su njihovi nosioci, onda je očekivano da frekvenca jedinki sa B hromozomima opada sa starošću. Analizirana je razlika u frekvenci B hromozoma u uzrasnim kategorijama kod 717 jedinki sa ciljem da se razjasne mehanizmi njihovog održavanja u populacijama A. flavicollis. Odsustvo statistički značajnih razlika u frekvenci B hromozoma između 6 uzrasnih kategorija ukazuje da se održavanje B hromozoma u populacijama ove vrste može objasniti njihovim doprinosom ukupnoj genetičkoj raznovrsnosti pre nego parazitskim ponašanjem.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Distribucija B hromozoma u uzrasnim kategorijama žutogrlog miša, Apodemus flavicollis (Mammalia, Rodentia)
T1  - Distribution of B chromosomes in age categories of the yellow-necked mouse Apodemus flavicollis (Mammalia, Rodentia)
IS  - 4
VL  - 61
DO  - 10.2298/ABS0904653V
SP  - 653
EP  - 658
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_236
ER  - 

@article{
author = "Vujošević, Mladen and Blagojević, Jelena and Jojić, Vida and Bugarski-Stanojević, Vanja and Adnađević, Tanja and Stamenković, Gorana",
year = "2009, 2009",
abstract = "The presence of B chromosomes (Bs) is frequently found in populations of yellow-necked mouse, Apodemus flavicollis. Differences in frequencies of Bs in age categories were analyzed in 717 animals in order to clarify the mechanisms of their maintenance in populations of A. flavicollis. The absence of statistically significant differences in the frequency of Bs between six age categories indicates that the maintenance of Bs in populations can be explained by in terms of their contribution to overall genetic diversity of the species rather than by parasitic behavior., Prekobrojni hromozomi nazivaju se B hromozomi ako zadovoljavaju tri kriterijuma: nisu neophodni za preživljavanje, ne sparuju se sa hromozomima A seta i ne nasleđuju se mendelovski. B hromozomi su vrlo često prisutni u populacijama žutogrlog miša, Apodemus flavicollis. Njihovo održavanje u populacijama objašnjava se sa dva suprostavljena modela. Model označen kao parazitski tvrdi da se B hromozomi održavaju zahvaljujući mehanizmu akumulacije uprkos štetnim efektima na fitnes nosilaca. S druge strane, heterotički model sugeriše da u odsustvu akumulacije mali broj B hromozoma ima adaptivnu prednost za nosioce, dok veći broj može biti štetan. Ako B hromozomi, koji su označeni kao parazitski, redukuju fitnes kod jedinki koje su njihovi nosioci, onda je očekivano da frekvenca jedinki sa B hromozomima opada sa starošću. Analizirana je razlika u frekvenci B hromozoma u uzrasnim kategorijama kod 717 jedinki sa ciljem da se razjasne mehanizmi njihovog održavanja u populacijama A. flavicollis. Odsustvo statistički značajnih razlika u frekvenci B hromozoma između 6 uzrasnih kategorija ukazuje da se održavanje B hromozoma u populacijama ove vrste može objasniti njihovim doprinosom ukupnoj genetičkoj raznovrsnosti pre nego parazitskim ponašanjem.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Distribucija B hromozoma u uzrasnim kategorijama žutogrlog miša, Apodemus flavicollis (Mammalia, Rodentia), Distribution of B chromosomes in age categories of the yellow-necked mouse Apodemus flavicollis (Mammalia, Rodentia)",
number = "4",
volume = "61",
doi = "10.2298/ABS0904653V",
pages = "653-658",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_236"
}

Vujošević, M., Blagojević, J., Jojić, V., Bugarski-Stanojević, V., Adnađević, T.,& Stamenković, G.. (2009). Distribucija B hromozoma u uzrasnim kategorijama žutogrlog miša, Apodemus flavicollis (Mammalia, Rodentia). in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 61(4), 653-658.
https://doi.org/10.2298/ABS0904653V
https://hdl.handle.net/21.15107/rcub_ibiss_236

Vujošević M, Blagojević J, Jojić V, Bugarski-Stanojević V, Adnađević T, Stamenković G. Distribucija B hromozoma u uzrasnim kategorijama žutogrlog miša, Apodemus flavicollis (Mammalia, Rodentia). in Archives of Biological Sciences. 2009;61(4):653-658.
doi:10.2298/ABS0904653V
https://hdl.handle.net/21.15107/rcub_ibiss_236 .

Vujošević, Mladen, Blagojević, Jelena, Jojić, Vida, Bugarski-Stanojević, Vanja, Adnađević, Tanja, Stamenković, Gorana, "Distribucija B hromozoma u uzrasnim kategorijama žutogrlog miša, Apodemus flavicollis (Mammalia, Rodentia)" in Archives of Biological Sciences, 61, no. 4 (2009):653-658,
https://doi.org/10.2298/ABS0904653V .,
https://hdl.handle.net/21.15107/rcub_ibiss_236 .

TY  - JOUR
AU  - Blagojević, Jelena
AU  - Stamenković, Gorana
AU  - Jojić, Vida
AU  - Bugarski-Stanojević, Vanja
AU  - Adnađević, Tanja
AU  - Vujošević, Mladen
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1480
AB  - B chromosomes (Bs) are supernumerary elements to the standard chromosome set, characterized by a dispensable nature. They are maintained like parasites if they possess any mechanism of accumulation. Otherwise, they must confer some selective advantage to the carriers. Some evidence for a beneficial effect led to the dominating opinion that Bs are genomic parasites. Populations of the yellow-necked mouse, Apodemus flavicollis, are characterized by a frequent presence of Bs, even though no mechanism for their accumulation has been found. Head morphology was compared in three populations of this species with different frequencies of animals with Bs (fB). Two of the localities (Mt. Avala, fB=0.37 and Mt. Cer, fB=0.31) are typical forest habitats and are about 100 km far from each other, while the third locality (Ada, fB=0.19) is a quite different habitat located less than 5 km from Mt. Cer. Discriminant analysis of morphological traits separated the locality Ada from Mt. Avala, while the population from Mt. Cer, besides its internal variation, included variation from both other localities (Ada-like and Mt. Avala-like). The frequency of animals with Bs in the Ada-like group (in the Mt. Cer population) was significantly different from that in the Ada population (0.19 vs. 0.43). Differences in fB, found in phenotypically/genotypically similar groups in different habitats, point to the existence of adaptive effects of Bs at the level of populations.
T2  - Italian Journal of Zoology
T1  - B chromosomes in populations of yellow-necked mice - stowaways or contributing genetic elements?
IS  - 3
VL  - 76
EP  - 257
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1480
ER  - 

@article{
author = "Blagojević, Jelena and Stamenković, Gorana and Jojić, Vida and Bugarski-Stanojević, Vanja and Adnađević, Tanja and Vujošević, Mladen",
year = "2009",
abstract = "B chromosomes (Bs) are supernumerary elements to the standard chromosome set, characterized by a dispensable nature. They are maintained like parasites if they possess any mechanism of accumulation. Otherwise, they must confer some selective advantage to the carriers. Some evidence for a beneficial effect led to the dominating opinion that Bs are genomic parasites. Populations of the yellow-necked mouse, Apodemus flavicollis, are characterized by a frequent presence of Bs, even though no mechanism for their accumulation has been found. Head morphology was compared in three populations of this species with different frequencies of animals with Bs (fB). Two of the localities (Mt. Avala, fB=0.37 and Mt. Cer, fB=0.31) are typical forest habitats and are about 100 km far from each other, while the third locality (Ada, fB=0.19) is a quite different habitat located less than 5 km from Mt. Cer. Discriminant analysis of morphological traits separated the locality Ada from Mt. Avala, while the population from Mt. Cer, besides its internal variation, included variation from both other localities (Ada-like and Mt. Avala-like). The frequency of animals with Bs in the Ada-like group (in the Mt. Cer population) was significantly different from that in the Ada population (0.19 vs. 0.43). Differences in fB, found in phenotypically/genotypically similar groups in different habitats, point to the existence of adaptive effects of Bs at the level of populations.",
journal = "Italian Journal of Zoology",
title = "B chromosomes in populations of yellow-necked mice - stowaways or contributing genetic elements?",
number = "3",
volume = "76",
pages = "257",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1480"
}

Blagojević, J., Stamenković, G., Jojić, V., Bugarski-Stanojević, V., Adnađević, T.,& Vujošević, M.. (2009). B chromosomes in populations of yellow-necked mice - stowaways or contributing genetic elements?. in Italian Journal of Zoology, 76(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1480

Blagojević J, Stamenković G, Jojić V, Bugarski-Stanojević V, Adnađević T, Vujošević M. B chromosomes in populations of yellow-necked mice - stowaways or contributing genetic elements?. in Italian Journal of Zoology. 2009;76(3):null-257.
https://hdl.handle.net/21.15107/rcub_ibiss_1480 .

Blagojević, Jelena, Stamenković, Gorana, Jojić, Vida, Bugarski-Stanojević, Vanja, Adnađević, Tanja, Vujošević, Mladen, "B chromosomes in populations of yellow-necked mice - stowaways or contributing genetic elements?" in Italian Journal of Zoology, 76, no. 3 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1480 .

TY  - JOUR
AU  - Bugarski-Stanojević, Vanja
AU  - Blagojević, Jelena
AU  - Adnađević, Tanja
AU  - Jojić, Vida
AU  - Vujošević, Mladen
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1522
AB  - Phylogenetic relationships among three species of the genus Apodemus (A.. avicollis, A. sylvaticus and A. agrarius) from seven different localities in Serbia were estimated using arbitrarily primed PCR (AP-PCR) pro. ling, which allowed clear distinction between the sibling species A.. avicollis and A. sylvaticus. Eight oligonucleotide primers out of the 22 screened, gained a total of 397 amplified bands. The average pairwise genetic distances within Apodemus species ranged from 0.51 between A.. avicollis and A. sylvaticus, to 0.74 between A. sylvaticus and A. agrarius. The large genetic distances obtained among populations of A.. avicollis (0.13) indicate that this part of the Balkan Peninsula was probably a refuge during the last glaciation. Furthermore, differences obtained between populations south and north from the river Danube suggest that this species survived the Last Glacial Maximum in at least two separate refuges. Although our data for molecular markers are from a limited number of localities, extended analyses of C-band distribution on chromosomes from previously studied localities south of the rivers Danube and Sava, indicate that distribution of A. sylvaticus is limited to northern Serbia. Therefore, it is possible that this part of the Balkan Peninsula was not a refuge for this species during the last glaciation.
T2  - Journal of Zoological Systematics and Evolutionary Research
T1  - Molecular phylogeny and distribution of three Apodemus species (Muridae, Rodentia) in Serbia
IS  - 3
VL  - 46
EP  - 286
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1522
ER  - 

@article{
author = "Bugarski-Stanojević, Vanja and Blagojević, Jelena and Adnađević, Tanja and Jojić, Vida and Vujošević, Mladen",
year = "2008",
abstract = "Phylogenetic relationships among three species of the genus Apodemus (A.. avicollis, A. sylvaticus and A. agrarius) from seven different localities in Serbia were estimated using arbitrarily primed PCR (AP-PCR) pro. ling, which allowed clear distinction between the sibling species A.. avicollis and A. sylvaticus. Eight oligonucleotide primers out of the 22 screened, gained a total of 397 amplified bands. The average pairwise genetic distances within Apodemus species ranged from 0.51 between A.. avicollis and A. sylvaticus, to 0.74 between A. sylvaticus and A. agrarius. The large genetic distances obtained among populations of A.. avicollis (0.13) indicate that this part of the Balkan Peninsula was probably a refuge during the last glaciation. Furthermore, differences obtained between populations south and north from the river Danube suggest that this species survived the Last Glacial Maximum in at least two separate refuges. Although our data for molecular markers are from a limited number of localities, extended analyses of C-band distribution on chromosomes from previously studied localities south of the rivers Danube and Sava, indicate that distribution of A. sylvaticus is limited to northern Serbia. Therefore, it is possible that this part of the Balkan Peninsula was not a refuge for this species during the last glaciation.",
journal = "Journal of Zoological Systematics and Evolutionary Research",
title = "Molecular phylogeny and distribution of three Apodemus species (Muridae, Rodentia) in Serbia",
number = "3",
volume = "46",
pages = "286",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1522"
}

Bugarski-Stanojević, V., Blagojević, J., Adnađević, T., Jojić, V.,& Vujošević, M.. (2008). Molecular phylogeny and distribution of three Apodemus species (Muridae, Rodentia) in Serbia. in Journal of Zoological Systematics and Evolutionary Research, 46(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1522

Bugarski-Stanojević V, Blagojević J, Adnađević T, Jojić V, Vujošević M. Molecular phylogeny and distribution of three Apodemus species (Muridae, Rodentia) in Serbia. in Journal of Zoological Systematics and Evolutionary Research. 2008;46(3):null-286.
https://hdl.handle.net/21.15107/rcub_ibiss_1522 .

Bugarski-Stanojević, Vanja, Blagojević, Jelena, Adnađević, Tanja, Jojić, Vida, Vujošević, Mladen, "Molecular phylogeny and distribution of three Apodemus species (Muridae, Rodentia) in Serbia" in Journal of Zoological Systematics and Evolutionary Research, 46, no. 3 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1522 .

TY  - JOUR
AU  - Vujošević, Mladen
AU  - Bugarski-Stanojević, Vanja
AU  - Jojić, Vida
AU  - Blagojević, Jelena
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1567
AB  - The correlation between frequency of B chromosomes and quality of habitat was analysed in 680 specimens of yellow-necked mice, Apodemus flavicollis, collected at six localities in Serbia. Animals with B chromosomes were present at all localities in frequencies ranging from 0.23 to 0.40. Quality of habitat was judged from an index of overall body size (BSI). At the studied localities, the frequency of animals with Bs was negatively correlated with the index of overall body size (r = -0.93), indicating that B chromosomes in this species are mentioned due to the effects that they exert at the level of populations. This finding supports the heterotic model of maintenance of B chromosomes.
T2  - Italian Journal of Zoology
T1  - Habitat quality and B chromosomes in the yellow-necked mouse Apodemus flavicollis
IS  - 4
VL  - 74
EP  - 316
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1567
ER  - 

@article{
author = "Vujošević, Mladen and Bugarski-Stanojević, Vanja and Jojić, Vida and Blagojević, Jelena",
year = "2007",
abstract = "The correlation between frequency of B chromosomes and quality of habitat was analysed in 680 specimens of yellow-necked mice, Apodemus flavicollis, collected at six localities in Serbia. Animals with B chromosomes were present at all localities in frequencies ranging from 0.23 to 0.40. Quality of habitat was judged from an index of overall body size (BSI). At the studied localities, the frequency of animals with Bs was negatively correlated with the index of overall body size (r = -0.93), indicating that B chromosomes in this species are mentioned due to the effects that they exert at the level of populations. This finding supports the heterotic model of maintenance of B chromosomes.",
journal = "Italian Journal of Zoology",
title = "Habitat quality and B chromosomes in the yellow-necked mouse Apodemus flavicollis",
number = "4",
volume = "74",
pages = "316",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1567"
}

Vujošević, M., Bugarski-Stanojević, V., Jojić, V.,& Blagojević, J.. (2007). Habitat quality and B chromosomes in the yellow-necked mouse Apodemus flavicollis. in Italian Journal of Zoology, 74(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1567

Vujošević M, Bugarski-Stanojević V, Jojić V, Blagojević J. Habitat quality and B chromosomes in the yellow-necked mouse Apodemus flavicollis. in Italian Journal of Zoology. 2007;74(4):null-316.
https://hdl.handle.net/21.15107/rcub_ibiss_1567 .

Vujošević, Mladen, Bugarski-Stanojević, Vanja, Jojić, Vida, Blagojević, Jelena, "Habitat quality and B chromosomes in the yellow-necked mouse Apodemus flavicollis" in Italian Journal of Zoology, 74, no. 4 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1567 .

TY  - JOUR
AU  - Jojić, Vida
AU  - Blagojević, Jelena
AU  - Ivanović, Ana
AU  - Bugarski-Stanojević, Vanja
AU  - Vujošević, Mladen
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1600
UR  - https://academic.oup.com/jmammal/article-lookup/doi/10.1644/06-MAMM-A-019R1.1
AB  - As a complex skeletal organ consisting of 2 functional and developmental units (ascending ramus and alveolar region), the mandible represents a well-established model in morphological integration studies. The concept of morphological integration assumes that developmentally or functionally related traits are more correlated than others and hence evolve together. We compared the level and pattern of mandibular morphological integration between groups of adult yellow-necked field mice (Apodemus flavicollis), with and without B chromosomes (Bs) in a population from Mt. Avala, Serbia. Bs are dispensable supernumerary chromosomes characterized by irregular and non-Mendelian modes of inheritance. The level of morphological integration was higher in animals with Bs. One of the 2 regions of the mandible tested (alveolar region) was significantly more affected by the presence of Bs than the other, with an increase in intensity of integration of 41.61% versus 15.86%. The hypothesis of morphological integration, which postulates disunion of the mandible into 2 distinct functional and developmental modules, was confirmed in animals with Bs. Bs probably have a function because they affect mandible phenotype (although the mechanism is unknown), increase variability within populations, and could lead to selective advantage.
T2  - Journal of Mammalogy
T1  - Morphological integration of the mandible in yellow-necked field mice: The effects of B chromosomes
IS  - 3
VL  - 88
DO  - 10.1644/06-MAMM-A-019R1.1
SP  - 689
EP  - 695
ER  - 

@article{
author = "Jojić, Vida and Blagojević, Jelena and Ivanović, Ana and Bugarski-Stanojević, Vanja and Vujošević, Mladen",
year = "2007",
abstract = "As a complex skeletal organ consisting of 2 functional and developmental units (ascending ramus and alveolar region), the mandible represents a well-established model in morphological integration studies. The concept of morphological integration assumes that developmentally or functionally related traits are more correlated than others and hence evolve together. We compared the level and pattern of mandibular morphological integration between groups of adult yellow-necked field mice (Apodemus flavicollis), with and without B chromosomes (Bs) in a population from Mt. Avala, Serbia. Bs are dispensable supernumerary chromosomes characterized by irregular and non-Mendelian modes of inheritance. The level of morphological integration was higher in animals with Bs. One of the 2 regions of the mandible tested (alveolar region) was significantly more affected by the presence of Bs than the other, with an increase in intensity of integration of 41.61% versus 15.86%. The hypothesis of morphological integration, which postulates disunion of the mandible into 2 distinct functional and developmental modules, was confirmed in animals with Bs. Bs probably have a function because they affect mandible phenotype (although the mechanism is unknown), increase variability within populations, and could lead to selective advantage.",
journal = "Journal of Mammalogy",
title = "Morphological integration of the mandible in yellow-necked field mice: The effects of B chromosomes",
number = "3",
volume = "88",
doi = "10.1644/06-MAMM-A-019R1.1",
pages = "689-695"
}

Jojić, V., Blagojević, J., Ivanović, A., Bugarski-Stanojević, V.,& Vujošević, M.. (2007). Morphological integration of the mandible in yellow-necked field mice: The effects of B chromosomes. in Journal of Mammalogy, 88(3), 689-695.
https://doi.org/10.1644/06-MAMM-A-019R1.1

Jojić V, Blagojević J, Ivanović A, Bugarski-Stanojević V, Vujošević M. Morphological integration of the mandible in yellow-necked field mice: The effects of B chromosomes. in Journal of Mammalogy. 2007;88(3):689-695.
doi:10.1644/06-MAMM-A-019R1.1 .

Jojić, Vida, Blagojević, Jelena, Ivanović, Ana, Bugarski-Stanojević, Vanja, Vujošević, Mladen, "Morphological integration of the mandible in yellow-necked field mice: The effects of B chromosomes" in Journal of Mammalogy, 88, no. 3 (2007):689-695,
https://doi.org/10.1644/06-MAMM-A-019R1.1 . .