TY  - JOUR
AU  - Stamenković, Gorana
AU  - Nikolic, V.
AU  - Blagojević, Jelena
AU  - Bugarski-Stanojević, Vanja
AU  - Adnađević, Tanja
AU  - Stanojevic, M.
AU  - Vujošević, Mladen
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1999
AB  - Dobrava-Belgrade virus (DOBV) is a hantavirus species that causes the
   most severe form of haemorrhagic fever with renal syndrome (HFRS) in
   Europe. DOBV has been detected in three Apodemus rodents: A.flavicollis,
   A.agrarius and A.ponticus. These emerging viruses appear throughout the
   Balkan Peninsula including Serbia as its central part. In this study, we
   examined the seroprevalence, molecular epidemiology and phylogenetics of
   DOBV from A.flavicollis captured at six Serbian localities. Furthermore,
   we applied microsatellite typing of host animal genome to analyse the
   role of host kinship in DOBV animal transmission. The overall IgG
   seropositivity rate over 3years (2008-2010) was 11.9\% (22/185). All
   seropositive samples were subjected to RT-PCR and DNA sequencing for S
   and L genome segments (pos. 291-1079nt and 2999-3316nt, respectively).
   DOBV was genetically detected in three samples from mountain Tara in
   western Serbia, a newly detected DOBV focus in the Balkans. No sequence
   data from human cases from Serbia are available for the studied period.
   However, collected DOBV isolates in this work phylogenetically clustered
   together with isolates from Serbian human cases dating from 2002, with
   1.9\% nucleotide divergence. We determined the level of kinship between
   seropositive and seronegative animal groups and found no significant
   difference, suggesting that horizontal virus transmission in the studied
   population was the same within and among the hatches. Our findings are
   the first genetic detection of DOBV in rodents in Serbia. We confirm
   wide and continuous hantavirus presence in the examined parts of the
   Balkans, underlying the necessity of continual monitoring of hantavirus
   circulation in A.flavicollis.
T2  - Zoonoses and Public Health
T1  - Genetic Analysis of Dobrava-Belgrade Virus from Western Serbia - A Newly
 Detected Focus in the Balkan Peninsula
IS  - 2
VL  - 62
DO  - 10.1111/zph.12136
SP  - 141
EP  - 150
ER  - 

@article{
author = "Stamenković, Gorana and Nikolic, V. and Blagojević, Jelena and Bugarski-Stanojević, Vanja and Adnađević, Tanja and Stanojevic, M. and Vujošević, Mladen",
year = "2015",
abstract = "Dobrava-Belgrade virus (DOBV) is a hantavirus species that causes the
   most severe form of haemorrhagic fever with renal syndrome (HFRS) in
   Europe. DOBV has been detected in three Apodemus rodents: A.flavicollis,
   A.agrarius and A.ponticus. These emerging viruses appear throughout the
   Balkan Peninsula including Serbia as its central part. In this study, we
   examined the seroprevalence, molecular epidemiology and phylogenetics of
   DOBV from A.flavicollis captured at six Serbian localities. Furthermore,
   we applied microsatellite typing of host animal genome to analyse the
   role of host kinship in DOBV animal transmission. The overall IgG
   seropositivity rate over 3years (2008-2010) was 11.9\% (22/185). All
   seropositive samples were subjected to RT-PCR and DNA sequencing for S
   and L genome segments (pos. 291-1079nt and 2999-3316nt, respectively).
   DOBV was genetically detected in three samples from mountain Tara in
   western Serbia, a newly detected DOBV focus in the Balkans. No sequence
   data from human cases from Serbia are available for the studied period.
   However, collected DOBV isolates in this work phylogenetically clustered
   together with isolates from Serbian human cases dating from 2002, with
   1.9\% nucleotide divergence. We determined the level of kinship between
   seropositive and seronegative animal groups and found no significant
   difference, suggesting that horizontal virus transmission in the studied
   population was the same within and among the hatches. Our findings are
   the first genetic detection of DOBV in rodents in Serbia. We confirm
   wide and continuous hantavirus presence in the examined parts of the
   Balkans, underlying the necessity of continual monitoring of hantavirus
   circulation in A.flavicollis.",
journal = "Zoonoses and Public Health",
title = "Genetic Analysis of Dobrava-Belgrade Virus from Western Serbia - A Newly
 Detected Focus in the Balkan Peninsula",
number = "2",
volume = "62",
doi = "10.1111/zph.12136",
pages = "141-150"
}

Stamenković, G., Nikolic, V., Blagojević, J., Bugarski-Stanojević, V., Adnađević, T., Stanojevic, M.,& Vujošević, M.. (2015). Genetic Analysis of Dobrava-Belgrade Virus from Western Serbia - A Newly
 Detected Focus in the Balkan Peninsula. in Zoonoses and Public Health, 62(2), 141-150.
https://doi.org/10.1111/zph.12136

Stamenković G, Nikolic V, Blagojević J, Bugarski-Stanojević V, Adnađević T, Stanojevic M, Vujošević M. Genetic Analysis of Dobrava-Belgrade Virus from Western Serbia - A Newly
 Detected Focus in the Balkan Peninsula. in Zoonoses and Public Health. 2015;62(2):141-150.
doi:10.1111/zph.12136 .

Stamenković, Gorana, Nikolic, V., Blagojević, Jelena, Bugarski-Stanojević, Vanja, Adnađević, Tanja, Stanojevic, M., Vujošević, Mladen, "Genetic Analysis of Dobrava-Belgrade Virus from Western Serbia - A Newly
 Detected Focus in the Balkan Peninsula" in Zoonoses and Public Health, 62, no. 2 (2015):141-150,
https://doi.org/10.1111/zph.12136 . .

TY  - JOUR
AU  - Stanojevic, M.
AU  - Nikolic, V.
AU  - Stajkovic, N.
AU  - Stamenković, Gorana
AU  - Bozovic, B.
AU  - Cekanac, R.
AU  - Marusic, P.
AU  - Gligic, A.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2091
AB  - Hantaviruses are endemic in the Balkans, particularly in Serbia, where
   sporadic cases and/or outbreaks of hantaviral human disease have been
   reported repeatedly, and evidenced serologically. Here, we present
   genetic detection of Dobrava-Belgrade virus (DOBV) hantaviral sequences
   in wild rodents trapped in central Serbia. All the animals were
   pre-screened serologically by indirect immunofluorescence (IF) test and
   only those with a positive finding of hantaviral antigens were further
   tested by polymerase chain reaction. Of the total of 104 trapped
   animals, 20 were found to be IF positive and of those three were
   positive for hantaviral RNA: one Microtus arvalis for Tula virus, and
   one each of Apodemus agrarius and Glis glis for DOBV. Phylogenetic
   analysis of the obtained sequences implies putative DOBV spillover
   infection of A. agrarius and G. glis from Apodemus flavicollis. However,
   future investigations should help to identify the most common natural
   host and geographical distribution of DOBV in its reservoir hosts in
   Serbia.
T2  - Epidemiology and Infection
T1  - Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse
 (Glis glis) in central Serbia
IS  - 2
VL  - 143
DO  - 10.1017/S0950268814001010
SP  - 400
EP  - 404
ER  - 

@article{
author = "Stanojevic, M. and Nikolic, V. and Stajkovic, N. and Stamenković, Gorana and Bozovic, B. and Cekanac, R. and Marusic, P. and Gligic, A.",
year = "2015",
abstract = "Hantaviruses are endemic in the Balkans, particularly in Serbia, where
   sporadic cases and/or outbreaks of hantaviral human disease have been
   reported repeatedly, and evidenced serologically. Here, we present
   genetic detection of Dobrava-Belgrade virus (DOBV) hantaviral sequences
   in wild rodents trapped in central Serbia. All the animals were
   pre-screened serologically by indirect immunofluorescence (IF) test and
   only those with a positive finding of hantaviral antigens were further
   tested by polymerase chain reaction. Of the total of 104 trapped
   animals, 20 were found to be IF positive and of those three were
   positive for hantaviral RNA: one Microtus arvalis for Tula virus, and
   one each of Apodemus agrarius and Glis glis for DOBV. Phylogenetic
   analysis of the obtained sequences implies putative DOBV spillover
   infection of A. agrarius and G. glis from Apodemus flavicollis. However,
   future investigations should help to identify the most common natural
   host and geographical distribution of DOBV in its reservoir hosts in
   Serbia.",
journal = "Epidemiology and Infection",
title = "Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse
 (Glis glis) in central Serbia",
number = "2",
volume = "143",
doi = "10.1017/S0950268814001010",
pages = "400-404"
}

Stanojevic, M., Nikolic, V., Stajkovic, N., Stamenković, G., Bozovic, B., Cekanac, R., Marusic, P.,& Gligic, A.. (2015). Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse
 (Glis glis) in central Serbia. in Epidemiology and Infection, 143(2), 400-404.
https://doi.org/10.1017/S0950268814001010

Stanojevic M, Nikolic V, Stajkovic N, Stamenković G, Bozovic B, Cekanac R, Marusic P, Gligic A. Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse
 (Glis glis) in central Serbia. in Epidemiology and Infection. 2015;143(2):400-404.
doi:10.1017/S0950268814001010 .

Stanojevic, M., Nikolic, V., Stajkovic, N., Stamenković, Gorana, Bozovic, B., Cekanac, R., Marusic, P., Gligic, A., "Genetic detection of Dobrava-Belgrade hantavirus in the edible dormouse
 (Glis glis) in central Serbia" in Epidemiology and Infection, 143, no. 2 (2015):400-404,
https://doi.org/10.1017/S0950268814001010 . .