TY  - CONF
AU  - Jonić, Natalija
AU  - Chatzigiannis, Christos M.
AU  - Koprivica, Ivan
AU  - Marinho, Sergio
AU  - Moura-Alves, Pedro
AU  - Pavić, Aleksandar
AU  - Otašević, Vesna
AU  - Pejnović, Nada
AU  - Tzakos, Andreas
AU  - Stojanović, Ivana D.
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6295
AB  - Introduction: The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor which is highly expressed in mucosal tissues - by epithelial cells and immune cells such as Th17 CD4+ and T regulatory cells (Treg). Besides its function of clearing environmental pollutants from the body, it was also revealed that AhR has immunoregulatory effects, thus becoming a potential therapeutic target for modulating the immune response. For that purpose we tested a novel synthetic AhR modulator under the code name C43.
Methods: CYP1A1 (downstream effector of AhR) activation was tested by the EROD assay. Sort-purified CD4+ cells from mesenteric lymph nodes (MLN) were treated with C43 for 24 h. Zebrafish embryos were used to test the toxicity of C43. Male C57BL/6 mice orally received C43 (10 mg/kg) for 5 consecutive days, after which MLN were harvested. Phenotype and function of the cells were analyzed by flow cytometry.
Results: C43 showed mild AhR agonistic activity. After treating the sort-purified CD4+ cells with C43, there was a shift in the Th17/Treg ratio in favour of the latter. C43 showed no signs of toxicity when tested on zebrafish embryos. MLN cells from mice that received C43 revealed a shift in the Th1/Treg ratio in favour of Tregs, with a documented rise of the portion of Tregs that expressed CYP1A1 in comparison with the control group of mice. 
Conclusion: C43 can modulate the immune response through the intestine by promoting the immunosuppressive Treg population.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
C3  - Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
T1  - Novel aryl hydrocarbon receptor modulator promotes immunosupressive immune response by stimulating T regulatory cells in the gut
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6295
ER  - 

@conference{
author = "Jonić, Natalija and Chatzigiannis, Christos M. and Koprivica, Ivan and Marinho, Sergio and Moura-Alves, Pedro and Pavić, Aleksandar and Otašević, Vesna and Pejnović, Nada and Tzakos, Andreas and Stojanović, Ivana D.",
year = "2023",
abstract = "Introduction: The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor which is highly expressed in mucosal tissues - by epithelial cells and immune cells such as Th17 CD4+ and T regulatory cells (Treg). Besides its function of clearing environmental pollutants from the body, it was also revealed that AhR has immunoregulatory effects, thus becoming a potential therapeutic target for modulating the immune response. For that purpose we tested a novel synthetic AhR modulator under the code name C43.
Methods: CYP1A1 (downstream effector of AhR) activation was tested by the EROD assay. Sort-purified CD4+ cells from mesenteric lymph nodes (MLN) were treated with C43 for 24 h. Zebrafish embryos were used to test the toxicity of C43. Male C57BL/6 mice orally received C43 (10 mg/kg) for 5 consecutive days, after which MLN were harvested. Phenotype and function of the cells were analyzed by flow cytometry.
Results: C43 showed mild AhR agonistic activity. After treating the sort-purified CD4+ cells with C43, there was a shift in the Th17/Treg ratio in favour of the latter. C43 showed no signs of toxicity when tested on zebrafish embryos. MLN cells from mice that received C43 revealed a shift in the Th1/Treg ratio in favour of Tregs, with a documented rise of the portion of Tregs that expressed CYP1A1 in comparison with the control group of mice. 
Conclusion: C43 can modulate the immune response through the intestine by promoting the immunosuppressive Treg population.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia",
title = "Novel aryl hydrocarbon receptor modulator promotes immunosupressive immune response by stimulating T regulatory cells in the gut",
pages = "38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6295"
}

Jonić, N., Chatzigiannis, C. M., Koprivica, I., Marinho, S., Moura-Alves, P., Pavić, A., Otašević, V., Pejnović, N., Tzakos, A.,& Stojanović, I. D.. (2023). Novel aryl hydrocarbon receptor modulator promotes immunosupressive immune response by stimulating T regulatory cells in the gut. in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade., 38.
https://hdl.handle.net/21.15107/rcub_ibiss_6295

Jonić N, Chatzigiannis CM, Koprivica I, Marinho S, Moura-Alves P, Pavić A, Otašević V, Pejnović N, Tzakos A, Stojanović ID. Novel aryl hydrocarbon receptor modulator promotes immunosupressive immune response by stimulating T regulatory cells in the gut. in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia. 2023;:38.
https://hdl.handle.net/21.15107/rcub_ibiss_6295 .

Jonić, Natalija, Chatzigiannis, Christos M., Koprivica, Ivan, Marinho, Sergio, Moura-Alves, Pedro, Pavić, Aleksandar, Otašević, Vesna, Pejnović, Nada, Tzakos, Andreas, Stojanović, Ivana D., "Novel aryl hydrocarbon receptor modulator promotes immunosupressive immune response by stimulating T regulatory cells in the gut" in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia (2023):38,
https://hdl.handle.net/21.15107/rcub_ibiss_6295 .

TY  - CONF
AU  - Jonić, Natalija
AU  - Chatzigiannis, Christos M.
AU  - Koprivica, Ivan
AU  - Marinho, Sergio
AU  - Moura-Alves, Pedro
AU  - Pavić, Aleksandar
AU  - Otašević, Vesna
AU  - Pejnović, Nada
AU  - Tzakos, Andreas
AU  - Stojanović, Ivana D.
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6296
AB  - The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has an important role in regulating the immune system, with high expression in Th17 CD4+ T cells and T regulatory cells (Treg). The expression of AhR is especially important at mucosal surfaces where it is involved in balancing the immune response towards external factors. The aim of our research was to evaluate the effect on the gut immune system of a novel fluorescent indole-containing compound that was designed as a putative AhR ligand (encoded C43). By using the EROD assay, we determined that C43 has mild AhR agonistic activity. Sort-purified mesenteric lymph node (MLN) CD4+ cells were treated with C43 for 24 h and flow cytometry analysis (FCM) showed that the Treg/Th17 ratio shifted in favour of Tregs. Zebrafish embryos were used for the evaluation of potential C43 toxicity. No nephrotoxicity, hepatotoxicity or cardiotoxicity was detected, even at the highest concentrations. Next, C43 was orally administered to healthy male C57BL/6 mice for 5 consecutive days, and later its effects on the gut immune system were recorded by analyzing the MLNs. FCM unveiled a higher proportion of Treg cells that expressed CYP1A1 (downstream effector of AhR) and the ratio of Treg/Th1 shifted towards Tregs. The presence of C43 was also visualized by confocal microscopy in the small intestine lamina propria of treated animals. With such results obtained from healthy animals, C43 presents a promising compound for the treatment of inflammatory diseases that generally involve activation of the gut immune system.
PB  - European Federation of Immunological Societies (EFIS)
C3  - Program and Abstracts: 12th EFIS-EJI South Eastern European Immunology School (SEEIS2023); 2023 Oct 20-23; Trogir, Croatia
T1  - Development of a novel compound that upregulates Treg in the gut by modulating aryl hydrocarbon receptor's activity
SP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6296
ER  - 

@conference{
author = "Jonić, Natalija and Chatzigiannis, Christos M. and Koprivica, Ivan and Marinho, Sergio and Moura-Alves, Pedro and Pavić, Aleksandar and Otašević, Vesna and Pejnović, Nada and Tzakos, Andreas and Stojanović, Ivana D.",
year = "2023",
abstract = "The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has an important role in regulating the immune system, with high expression in Th17 CD4+ T cells and T regulatory cells (Treg). The expression of AhR is especially important at mucosal surfaces where it is involved in balancing the immune response towards external factors. The aim of our research was to evaluate the effect on the gut immune system of a novel fluorescent indole-containing compound that was designed as a putative AhR ligand (encoded C43). By using the EROD assay, we determined that C43 has mild AhR agonistic activity. Sort-purified mesenteric lymph node (MLN) CD4+ cells were treated with C43 for 24 h and flow cytometry analysis (FCM) showed that the Treg/Th17 ratio shifted in favour of Tregs. Zebrafish embryos were used for the evaluation of potential C43 toxicity. No nephrotoxicity, hepatotoxicity or cardiotoxicity was detected, even at the highest concentrations. Next, C43 was orally administered to healthy male C57BL/6 mice for 5 consecutive days, and later its effects on the gut immune system were recorded by analyzing the MLNs. FCM unveiled a higher proportion of Treg cells that expressed CYP1A1 (downstream effector of AhR) and the ratio of Treg/Th1 shifted towards Tregs. The presence of C43 was also visualized by confocal microscopy in the small intestine lamina propria of treated animals. With such results obtained from healthy animals, C43 presents a promising compound for the treatment of inflammatory diseases that generally involve activation of the gut immune system.",
publisher = "European Federation of Immunological Societies (EFIS)",
journal = "Program and Abstracts: 12th EFIS-EJI South Eastern European Immunology School (SEEIS2023); 2023 Oct 20-23; Trogir, Croatia",
title = "Development of a novel compound that upregulates Treg in the gut by modulating aryl hydrocarbon receptor's activity",
pages = "17",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6296"
}

Jonić, N., Chatzigiannis, C. M., Koprivica, I., Marinho, S., Moura-Alves, P., Pavić, A., Otašević, V., Pejnović, N., Tzakos, A.,& Stojanović, I. D.. (2023). Development of a novel compound that upregulates Treg in the gut by modulating aryl hydrocarbon receptor's activity. in Program and Abstracts: 12th EFIS-EJI South Eastern European Immunology School (SEEIS2023); 2023 Oct 20-23; Trogir, Croatia
European Federation of Immunological Societies (EFIS)., 17.
https://hdl.handle.net/21.15107/rcub_ibiss_6296

Jonić N, Chatzigiannis CM, Koprivica I, Marinho S, Moura-Alves P, Pavić A, Otašević V, Pejnović N, Tzakos A, Stojanović ID. Development of a novel compound that upregulates Treg in the gut by modulating aryl hydrocarbon receptor's activity. in Program and Abstracts: 12th EFIS-EJI South Eastern European Immunology School (SEEIS2023); 2023 Oct 20-23; Trogir, Croatia. 2023;:17.
https://hdl.handle.net/21.15107/rcub_ibiss_6296 .

Jonić, Natalija, Chatzigiannis, Christos M., Koprivica, Ivan, Marinho, Sergio, Moura-Alves, Pedro, Pavić, Aleksandar, Otašević, Vesna, Pejnović, Nada, Tzakos, Andreas, Stojanović, Ivana D., "Development of a novel compound that upregulates Treg in the gut by modulating aryl hydrocarbon receptor's activity" in Program and Abstracts: 12th EFIS-EJI South Eastern European Immunology School (SEEIS2023); 2023 Oct 20-23; Trogir, Croatia (2023):17,
https://hdl.handle.net/21.15107/rcub_ibiss_6296 .

TY  - CONF
AU  - Jonić, Natalija
AU  - Chatzigiannis, Christos M
AU  - Koprivica, Ivan
AU  - Marinho, Sérgio
AU  - Moura-Alves, Pedro
AU  - Pavić, Aleksandar
AU  - Dimitrijević, Mirjana
AU  - Jovanović, Anđelina
AU  - Jovanović, Milan B
AU  - Otašević, Vesna
AU  - Pejnović, Nada
AU  - Tzakos, Andreas
AU  - Stojanović, Ivana D.
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5731
AB  - Aril ugljovodonični receptor (AhR) je transkripcioni faktor aktiviran ligandom i prevashodno je eksprimiran u imunskom tkivu creva. Kako istraživanja ukazuju na povezanost mukoznog imuniteta i različitih inflamatornih i autoimunskih oboljenja, ispitivali smo modulaciju imunskih ćelija creva pomoću novosintetisanog liganda AhR (šifra C43). Primenjen u kulturi mišjih CD4+ ćelija izolovanih iz mezenteričnih limfnih čvorova (MLČ), kao i na humanim ćelijama izolovanih iz tonzila, C43 je značajno uvećao udeo Treg nakon 24h. Nakon što je pokazano da C43 ne ostvaruje toksičnost (ni pri najvećim koncentracijama) pri razviću embriona zebrica (lat. Danio rerio), gavažom je 5 dana davan zdravim C57BL/6 mužjacima. U odnosu zastupljenosti Th1/Treg u MLČ, uočeno je pomeranje balansa ka Treg, kao i povećanje udela Treg koje eksprimiraju Cyp1a1 (nishodni signalni molekul od AhR) kod tretiranih miševa. Kada je C43 gavažom davan C57BL/6 mužjacima kojima je dijabetes tipa 1 (DT1) indukovan streptozotocinom, glikemijski indeksi su bili niži, a histološka analiza pankreasa je pokazala bolje očuvanje β ćelija i pankreasnih ostrvaca. Analiza lamine proprije tankog creva je pokazala povećanje udela tolerogenih dendritskih ćelija (tolDC), dok je udeo CD11b+MHCII+ ćelija bio snižen. Udeo Treg je takođe bio veći, kao i Cyp1a1+ Treg i IL-10+ Treg. Analizom pankreasnog limfnog čvora uočeno je sniženje udela Th1 i CD8+ ćelija, uz povećanje udela tolDC koje eksprimiraju indolamin 2,3-dioksigenazu, što je zabeleženo i u inflitratima pankreasa. Na osnovu dobijenih rezultata može se zaključiti da C43 ostvaruje antiinflamatorni efekat u DT1 i da pristup stimulacije AhR u mukozi creva može imati povoljan efekat u modulaciji autoimunosti i/ili inflamatornih oboljenja.
PB  - Belgrade: Serbian Academy of Sciences and Arts
C3  - Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia
T1  - Novosintetisani fluorescentni AhR ligand podstiče povećanje udela T regulatornih ćelija i ublažava kliničku sliku dijabetesa tipa 1 kod C57BL/6 miševa
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5731
ER  - 

@conference{
author = "Jonić, Natalija and Chatzigiannis, Christos M and Koprivica, Ivan and Marinho, Sérgio and Moura-Alves, Pedro and Pavić, Aleksandar and Dimitrijević, Mirjana and Jovanović, Anđelina and Jovanović, Milan B and Otašević, Vesna and Pejnović, Nada and Tzakos, Andreas and Stojanović, Ivana D.",
year = "2023",
abstract = "Aril ugljovodonični receptor (AhR) je transkripcioni faktor aktiviran ligandom i prevashodno je eksprimiran u imunskom tkivu creva. Kako istraživanja ukazuju na povezanost mukoznog imuniteta i različitih inflamatornih i autoimunskih oboljenja, ispitivali smo modulaciju imunskih ćelija creva pomoću novosintetisanog liganda AhR (šifra C43). Primenjen u kulturi mišjih CD4+ ćelija izolovanih iz mezenteričnih limfnih čvorova (MLČ), kao i na humanim ćelijama izolovanih iz tonzila, C43 je značajno uvećao udeo Treg nakon 24h. Nakon što je pokazano da C43 ne ostvaruje toksičnost (ni pri najvećim koncentracijama) pri razviću embriona zebrica (lat. Danio rerio), gavažom je 5 dana davan zdravim C57BL/6 mužjacima. U odnosu zastupljenosti Th1/Treg u MLČ, uočeno je pomeranje balansa ka Treg, kao i povećanje udela Treg koje eksprimiraju Cyp1a1 (nishodni signalni molekul od AhR) kod tretiranih miševa. Kada je C43 gavažom davan C57BL/6 mužjacima kojima je dijabetes tipa 1 (DT1) indukovan streptozotocinom, glikemijski indeksi su bili niži, a histološka analiza pankreasa je pokazala bolje očuvanje β ćelija i pankreasnih ostrvaca. Analiza lamine proprije tankog creva je pokazala povećanje udela tolerogenih dendritskih ćelija (tolDC), dok je udeo CD11b+MHCII+ ćelija bio snižen. Udeo Treg je takođe bio veći, kao i Cyp1a1+ Treg i IL-10+ Treg. Analizom pankreasnog limfnog čvora uočeno je sniženje udela Th1 i CD8+ ćelija, uz povećanje udela tolDC koje eksprimiraju indolamin 2,3-dioksigenazu, što je zabeleženo i u inflitratima pankreasa. Na osnovu dobijenih rezultata može se zaključiti da C43 ostvaruje antiinflamatorni efekat u DT1 i da pristup stimulacije AhR u mukozi creva može imati povoljan efekat u modulaciji autoimunosti i/ili inflamatornih oboljenja.",
publisher = "Belgrade: Serbian Academy of Sciences and Arts",
journal = "Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia",
title = "Novosintetisani fluorescentni AhR ligand podstiče povećanje udela T regulatornih ćelija i ublažava kliničku sliku dijabetesa tipa 1 kod C57BL/6 miševa",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5731"
}

Jonić, N., Chatzigiannis, C. M., Koprivica, I., Marinho, S., Moura-Alves, P., Pavić, A., Dimitrijević, M., Jovanović, A., Jovanović, M. B., Otašević, V., Pejnović, N., Tzakos, A.,& Stojanović, I. D.. (2023). Novosintetisani fluorescentni AhR ligand podstiče povećanje udela T regulatornih ćelija i ublažava kliničku sliku dijabetesa tipa 1 kod C57BL/6 miševa. in Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia
Belgrade: Serbian Academy of Sciences and Arts..
https://hdl.handle.net/21.15107/rcub_ibiss_5731

Jonić N, Chatzigiannis CM, Koprivica I, Marinho S, Moura-Alves P, Pavić A, Dimitrijević M, Jovanović A, Jovanović MB, Otašević V, Pejnović N, Tzakos A, Stojanović ID. Novosintetisani fluorescentni AhR ligand podstiče povećanje udela T regulatornih ćelija i ublažava kliničku sliku dijabetesa tipa 1 kod C57BL/6 miševa. in Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_5731 .

Jonić, Natalija, Chatzigiannis, Christos M, Koprivica, Ivan, Marinho, Sérgio, Moura-Alves, Pedro, Pavić, Aleksandar, Dimitrijević, Mirjana, Jovanović, Anđelina, Jovanović, Milan B, Otašević, Vesna, Pejnović, Nada, Tzakos, Andreas, Stojanović, Ivana D., "Novosintetisani fluorescentni AhR ligand podstiče povećanje udela T regulatornih ćelija i ublažava kliničku sliku dijabetesa tipa 1 kod C57BL/6 miševa" in Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5731 .

TY  - JOUR
AU  - Pavić, Aleksandar
AU  - Ilić-Tomić, Tatjana
AU  - Glamočlija, Jasmina
PY  - 2021
UR  - https://www.mdpi.com/2309-608X/7/10/834
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8540621
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4634
AB  - Severe drawbacks associated with the topical use of depigmenting agents in treatments of skin hyperigmentations impose a great demand for novel, effective, and safe melanogenesis inhibitors. Edible and medicinal mushrooms, known for numerous health-promoting properties, represent a rich reservoir of anti-melanogenic compounds, with the potential to be applied in preventing excessive skin pigmentation. Herein, using zebrafish (Danio rerio) as a preclinical animal model, we have demonstrated that ethanol extract of Laetiporus sulphureus (LSE) and Agaricus silvaticus (ASE) are not toxic at high doses up to 400-500 µg/mL while effectively inhibit melanogenesis in a dose-dependent manner. At depigmenting doses, the explored extracts showed no adverse effects on zebrafish embryos melanocytes. Even more, they did not provoke inflammation or neutropenia when applied at the highest dose ensuring almost complete the cells depigmentation. Since LSE and ASE have demonstrated significantly higher the therapeutic potential than kojic acid and hydroquinone, two well-known depigmenting agents, overall results of this study strongly suggest that the explored mushrooms extracts could be used as efficient and safe topical agents in treatments of skin hyperpigmentation disorders.
PB  - Basel: MDPI
T2  - Journal of Fungi
T1  - Unravelling Anti-Melanogenic Potency of Edible Mushrooms Laetiporus sulphureus and Agaricus silvaticus In Vivo Using the Zebrafish Model.
IS  - 10
VL  - 7
DO  - 10.3390/jof7100834
SP  - 834
ER  - 

@article{
author = "Pavić, Aleksandar and Ilić-Tomić, Tatjana and Glamočlija, Jasmina",
year = "2021",
abstract = "Severe drawbacks associated with the topical use of depigmenting agents in treatments of skin hyperigmentations impose a great demand for novel, effective, and safe melanogenesis inhibitors. Edible and medicinal mushrooms, known for numerous health-promoting properties, represent a rich reservoir of anti-melanogenic compounds, with the potential to be applied in preventing excessive skin pigmentation. Herein, using zebrafish (Danio rerio) as a preclinical animal model, we have demonstrated that ethanol extract of Laetiporus sulphureus (LSE) and Agaricus silvaticus (ASE) are not toxic at high doses up to 400-500 µg/mL while effectively inhibit melanogenesis in a dose-dependent manner. At depigmenting doses, the explored extracts showed no adverse effects on zebrafish embryos melanocytes. Even more, they did not provoke inflammation or neutropenia when applied at the highest dose ensuring almost complete the cells depigmentation. Since LSE and ASE have demonstrated significantly higher the therapeutic potential than kojic acid and hydroquinone, two well-known depigmenting agents, overall results of this study strongly suggest that the explored mushrooms extracts could be used as efficient and safe topical agents in treatments of skin hyperpigmentation disorders.",
publisher = "Basel: MDPI",
journal = "Journal of Fungi",
title = "Unravelling Anti-Melanogenic Potency of Edible Mushrooms Laetiporus sulphureus and Agaricus silvaticus In Vivo Using the Zebrafish Model.",
number = "10",
volume = "7",
doi = "10.3390/jof7100834",
pages = "834"
}

Pavić, A., Ilić-Tomić, T.,& Glamočlija, J.. (2021). Unravelling Anti-Melanogenic Potency of Edible Mushrooms Laetiporus sulphureus and Agaricus silvaticus In Vivo Using the Zebrafish Model.. in Journal of Fungi
Basel: MDPI., 7(10), 834.
https://doi.org/10.3390/jof7100834

Pavić A, Ilić-Tomić T, Glamočlija J. Unravelling Anti-Melanogenic Potency of Edible Mushrooms Laetiporus sulphureus and Agaricus silvaticus In Vivo Using the Zebrafish Model.. in Journal of Fungi. 2021;7(10):834.
doi:10.3390/jof7100834 .

Pavić, Aleksandar, Ilić-Tomić, Tatjana, Glamočlija, Jasmina, "Unravelling Anti-Melanogenic Potency of Edible Mushrooms Laetiporus sulphureus and Agaricus silvaticus In Vivo Using the Zebrafish Model." in Journal of Fungi, 7, no. 10 (2021):834,
https://doi.org/10.3390/jof7100834 . .

TY  - CONF
AU  - Petrović, Jovana
AU  - Glamočlija, Jasmina
AU  - Ilić-Tomić, Tatjana
AU  - Soković, Marina
AU  - Robajac, Dragana
AU  - Nedić, Olgica
AU  - Pavić, Aleksandar
PY  - 2020
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6535
AB  - Laetiporus sulphureus, an edible mushroom, for centuries has been used in traditional 
European and Asian ethno-medicine. Its therapeutic properties have been attributed to different 
types of biologically active compounds, such as terpenes, polysaccharides, steroids, proteins 
and lectins. Recently, mushroom lectins emerged as very potent macromolecules with 
antiproliferative, cytotoxic, antiviral and immunostimulatory activities. Therefore, we isolated 
lectin from L. sulphureus (LSL) and evaluated its anti-angiogenic and anticancer activity in 
vivo, using the zebrafish model. We found that LSL is not toxic at high doses up to 400-
500 μg/mL, while it effectively inhibited angiogenesis and cancer development at much lower 
doses. Compared to sunitinib-malate, cardiotoxic and myelosupressive anti-angiogenic drug of 
clinical relevance, therapeutic potential of LSL was 378-fold higher. Wound healing and MTT 
assays were employed to examine antimigratory effect and endothelial cytotoxicity. 
Surprisingly, LSL affected human colorectal carcinoma and mouse melanoma cell lines, by 
almost completely diminishing their growth, neovascularization and metastasis. Moreover, in 
comparison to the used control (cisplatin), LSL showed markedly greater activity, while its 
potency turned out to be 8-fold higher towards colorectal carcinoma than melanoma. These 
encouraging data strongly imply that LSL can be considered for the application as supporting 
agent in chemotherapy against colorectal carcinoma and melanoma or used in pharmaceutical 
industry.
PB  - Belgrade: NutRedox, COST Action CA16112
C3  - WGs Meeting of the NutRedOx COST Action CA16112: Nutraceuticals in balancing redox status in ageing and age-related diseases; 2020 Mar 2-3; Belgrade, Serbia
T1  - Laetiporus sulphureus lectin inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.
SP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6535
ER  - 

@conference{
author = "Petrović, Jovana and Glamočlija, Jasmina and Ilić-Tomić, Tatjana and Soković, Marina and Robajac, Dragana and Nedić, Olgica and Pavić, Aleksandar",
year = "2020",
abstract = "Laetiporus sulphureus, an edible mushroom, for centuries has been used in traditional 
European and Asian ethno-medicine. Its therapeutic properties have been attributed to different 
types of biologically active compounds, such as terpenes, polysaccharides, steroids, proteins 
and lectins. Recently, mushroom lectins emerged as very potent macromolecules with 
antiproliferative, cytotoxic, antiviral and immunostimulatory activities. Therefore, we isolated 
lectin from L. sulphureus (LSL) and evaluated its anti-angiogenic and anticancer activity in 
vivo, using the zebrafish model. We found that LSL is not toxic at high doses up to 400-
500 μg/mL, while it effectively inhibited angiogenesis and cancer development at much lower 
doses. Compared to sunitinib-malate, cardiotoxic and myelosupressive anti-angiogenic drug of 
clinical relevance, therapeutic potential of LSL was 378-fold higher. Wound healing and MTT 
assays were employed to examine antimigratory effect and endothelial cytotoxicity. 
Surprisingly, LSL affected human colorectal carcinoma and mouse melanoma cell lines, by 
almost completely diminishing their growth, neovascularization and metastasis. Moreover, in 
comparison to the used control (cisplatin), LSL showed markedly greater activity, while its 
potency turned out to be 8-fold higher towards colorectal carcinoma than melanoma. These 
encouraging data strongly imply that LSL can be considered for the application as supporting 
agent in chemotherapy against colorectal carcinoma and melanoma or used in pharmaceutical 
industry.",
publisher = "Belgrade: NutRedox, COST Action CA16112",
journal = "WGs Meeting of the NutRedOx COST Action CA16112: Nutraceuticals in balancing redox status in ageing and age-related diseases; 2020 Mar 2-3; Belgrade, Serbia",
title = "Laetiporus sulphureus lectin inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.",
pages = "43",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6535"
}

Petrović, J., Glamočlija, J., Ilić-Tomić, T., Soković, M., Robajac, D., Nedić, O.,& Pavić, A.. (2020). Laetiporus sulphureus lectin inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.. in WGs Meeting of the NutRedOx COST Action CA16112: Nutraceuticals in balancing redox status in ageing and age-related diseases; 2020 Mar 2-3; Belgrade, Serbia
Belgrade: NutRedox, COST Action CA16112., 43.
https://hdl.handle.net/21.15107/rcub_ibiss_6535

Petrović J, Glamočlija J, Ilić-Tomić T, Soković M, Robajac D, Nedić O, Pavić A. Laetiporus sulphureus lectin inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.. in WGs Meeting of the NutRedOx COST Action CA16112: Nutraceuticals in balancing redox status in ageing and age-related diseases; 2020 Mar 2-3; Belgrade, Serbia. 2020;:43.
https://hdl.handle.net/21.15107/rcub_ibiss_6535 .

Petrović, Jovana, Glamočlija, Jasmina, Ilić-Tomić, Tatjana, Soković, Marina, Robajac, Dragana, Nedić, Olgica, Pavić, Aleksandar, "Laetiporus sulphureus lectin inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma." in WGs Meeting of the NutRedOx COST Action CA16112: Nutraceuticals in balancing redox status in ageing and age-related diseases; 2020 Mar 2-3; Belgrade, Serbia (2020):43,
https://hdl.handle.net/21.15107/rcub_ibiss_6535 .

TY  - JOUR
AU  - Petrović, Jovana
AU  - Glamočlija, Jasmina
AU  - Ilić-Tomić, Tatjana
AU  - Soković, Marina
AU  - Robajac, Dragana
AU  - Nedić, Olgica
AU  - Pavić, Aleksandar
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3603
AB  - In spite of extensive usage of Laetiporus sulphureus (sulphur polypore) in traditional European and Asian ethno-medicine for centuries, its anticancer therapeutic potential and toxicity profile remained explored in animal models. Herein, using zebrafish (Danio rerio), as a preclinical animal model, we demonstrated that L. sulphureus lectin (LSL) and ethanol extract (LSE) are non-toxic at high doses up to 400-500 μg/mL, while they effectively inhibited angiogenesis and cancer development at much lower doses. Lectin showed 74-fold higher anti-angiogenic potency than the extract, and even 378-fold higher therapeutic potential than sunitinib-malate, cardiotoxic and myelosupressive anti-angiogenic drug of clinical relevance. Using wound healing and MTT assays, we proved LSL's strong antimigratory effect and selective endothelial cytotoxicity in relation to lung fibroblasts. In addition, employing the zebrafish xenograft models, we demonstrated that LSL almost completely reduced growth, neovascularization and metastasis of human colorectal carcinoma and mouse melanoma. Even more, LSL exerted 8-fold higher potency towards colorectal carcinoma than melanoma, showing markedly higher activity than cisplatin, while LSE failed to express any anticancer activity. Accompanied with non-toxic response, including neutropenia and inflammation, the results of this study strongly imply that LSL could be used as safe adjuvant in chemotherapy against colorectal carcinoma and melanoma.
T2  - International Journal of Biological Macromolecules
T1  - Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.
VL  - 148
DO  - 10.1016/j.ijbiomac.2020.01.033
SP  - 129
EP  - 139
ER  - 

@article{
author = "Petrović, Jovana and Glamočlija, Jasmina and Ilić-Tomić, Tatjana and Soković, Marina and Robajac, Dragana and Nedić, Olgica and Pavić, Aleksandar",
year = "2020",
abstract = "In spite of extensive usage of Laetiporus sulphureus (sulphur polypore) in traditional European and Asian ethno-medicine for centuries, its anticancer therapeutic potential and toxicity profile remained explored in animal models. Herein, using zebrafish (Danio rerio), as a preclinical animal model, we demonstrated that L. sulphureus lectin (LSL) and ethanol extract (LSE) are non-toxic at high doses up to 400-500 μg/mL, while they effectively inhibited angiogenesis and cancer development at much lower doses. Lectin showed 74-fold higher anti-angiogenic potency than the extract, and even 378-fold higher therapeutic potential than sunitinib-malate, cardiotoxic and myelosupressive anti-angiogenic drug of clinical relevance. Using wound healing and MTT assays, we proved LSL's strong antimigratory effect and selective endothelial cytotoxicity in relation to lung fibroblasts. In addition, employing the zebrafish xenograft models, we demonstrated that LSL almost completely reduced growth, neovascularization and metastasis of human colorectal carcinoma and mouse melanoma. Even more, LSL exerted 8-fold higher potency towards colorectal carcinoma than melanoma, showing markedly higher activity than cisplatin, while LSE failed to express any anticancer activity. Accompanied with non-toxic response, including neutropenia and inflammation, the results of this study strongly imply that LSL could be used as safe adjuvant in chemotherapy against colorectal carcinoma and melanoma.",
journal = "International Journal of Biological Macromolecules",
title = "Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.",
volume = "148",
doi = "10.1016/j.ijbiomac.2020.01.033",
pages = "129-139"
}

Petrović, J., Glamočlija, J., Ilić-Tomić, T., Soković, M., Robajac, D., Nedić, O.,& Pavić, A.. (2020). Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.. in International Journal of Biological Macromolecules, 148, 129-139.
https://doi.org/10.1016/j.ijbiomac.2020.01.033

Petrović J, Glamočlija J, Ilić-Tomić T, Soković M, Robajac D, Nedić O, Pavić A. Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma.. in International Journal of Biological Macromolecules. 2020;148:129-139.
doi:10.1016/j.ijbiomac.2020.01.033 .

Petrović, Jovana, Glamočlija, Jasmina, Ilić-Tomić, Tatjana, Soković, Marina, Robajac, Dragana, Nedić, Olgica, Pavić, Aleksandar, "Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma." in International Journal of Biological Macromolecules, 148 (2020):129-139,
https://doi.org/10.1016/j.ijbiomac.2020.01.033 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Kovačević-Grujičić, Nataša
AU  - Reis, Filipa S.
AU  - Davidović, Slobodan
AU  - Barros, Lillian
AU  - Popović, Jelena
AU  - Petrović, Isidora
AU  - Pavić, Aleksandar
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Stevanović, Milena
AU  - Ferreira, Isabel C.F.R.
AU  - Soković, Marina
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0023643817300452
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2580
AB  - Wild Meripilus giganteus Karst belongs to the order Polyporales, in which some members are known to possess a wide range of pharmacological properties. M. giganteus showed to be rich in carbohydrates (74.49 g/100 g) and proteins (15.94 g/100 g), presenting low fat content (1.51 g/100 g). Chemical composition was determined by using chromatographic techniques. Also, various bioactive compounds were detected including all four tocopherol isoforms with δ- and γ-tocopherols being predominant (123.35 and 77.80 μg/100 g, respectively); five organic acids (oxalic, malic, quinic, citric and fumaric acids) with predominant malic acid (3.17 g/100 g); and three phenolic acids and related compounds (p-hydroxybenzoic, p-coumaric and cinnamic acids; 1010, 2420 and 340 μg/100 g, respectively). M. giganteus methanolic extract exhibited antioxidant activity tested by five different assays with the strongest potential in TBARS assay (EC50 0.31 mg/mL); and antimicrobial activities (MIC/MBC 0.0125–5 mg/mL; MIC/MFC 0.025–0.4 mg/mL). Furthermore, treatment of cervical carcinoma cell line (HeLa) led to reduction in cell's viability in MTT assay (IC50 0.41 mg/mL after 48 h), induced process of apoptosis and inhibited cell's migration in vitro. The analysed extract was not toxic for zebrafish embryos (at 0.5 mg/mL), indicating its biosafety and potential application as a dietary supplement in chemoprevention.
T2  - LWT - Food Science and Technology
T1  - Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract
VL  - 79
DO  - 10.1016/j.lwt.2017.01.045
SP  - 454
EP  - 462
ER  - 

@article{
author = "Stojković, Dejan and Kovačević-Grujičić, Nataša and Reis, Filipa S. and Davidović, Slobodan and Barros, Lillian and Popović, Jelena and Petrović, Isidora and Pavić, Aleksandar and Glamočlija, Jasmina and Ćirić, Ana and Stevanović, Milena and Ferreira, Isabel C.F.R. and Soković, Marina",
year = "2017",
abstract = "Wild Meripilus giganteus Karst belongs to the order Polyporales, in which some members are known to possess a wide range of pharmacological properties. M. giganteus showed to be rich in carbohydrates (74.49 g/100 g) and proteins (15.94 g/100 g), presenting low fat content (1.51 g/100 g). Chemical composition was determined by using chromatographic techniques. Also, various bioactive compounds were detected including all four tocopherol isoforms with δ- and γ-tocopherols being predominant (123.35 and 77.80 μg/100 g, respectively); five organic acids (oxalic, malic, quinic, citric and fumaric acids) with predominant malic acid (3.17 g/100 g); and three phenolic acids and related compounds (p-hydroxybenzoic, p-coumaric and cinnamic acids; 1010, 2420 and 340 μg/100 g, respectively). M. giganteus methanolic extract exhibited antioxidant activity tested by five different assays with the strongest potential in TBARS assay (EC50 0.31 mg/mL); and antimicrobial activities (MIC/MBC 0.0125–5 mg/mL; MIC/MFC 0.025–0.4 mg/mL). Furthermore, treatment of cervical carcinoma cell line (HeLa) led to reduction in cell's viability in MTT assay (IC50 0.41 mg/mL after 48 h), induced process of apoptosis and inhibited cell's migration in vitro. The analysed extract was not toxic for zebrafish embryos (at 0.5 mg/mL), indicating its biosafety and potential application as a dietary supplement in chemoprevention.",
journal = "LWT - Food Science and Technology",
title = "Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract",
volume = "79",
doi = "10.1016/j.lwt.2017.01.045",
pages = "454-462"
}

Stojković, D., Kovačević-Grujičić, N., Reis, F. S., Davidović, S., Barros, L., Popović, J., Petrović, I., Pavić, A., Glamočlija, J., Ćirić, A., Stevanović, M., Ferreira, I. C.F.R.,& Soković, M.. (2017). Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract. in LWT - Food Science and Technology, 79, 454-462.
https://doi.org/10.1016/j.lwt.2017.01.045

Stojković D, Kovačević-Grujičić N, Reis FS, Davidović S, Barros L, Popović J, Petrović I, Pavić A, Glamočlija J, Ćirić A, Stevanović M, Ferreira IC, Soković M. Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract. in LWT - Food Science and Technology. 2017;79:454-462.
doi:10.1016/j.lwt.2017.01.045 .

Stojković, Dejan, Kovačević-Grujičić, Nataša, Reis, Filipa S., Davidović, Slobodan, Barros, Lillian, Popović, Jelena, Petrović, Isidora, Pavić, Aleksandar, Glamočlija, Jasmina, Ćirić, Ana, Stevanović, Milena, Ferreira, Isabel C.F.R., Soković, Marina, "Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract" in LWT - Food Science and Technology, 79 (2017):454-462,
https://doi.org/10.1016/j.lwt.2017.01.045 . .