TY  - CONF
AU  - Pajović, Milica
AU  - Stanković Jeremić, Jovana
AU  - Jovanović Stojanov, Sofija
AU  - Gođevac, Dejan
AU  - Pešić, Milica
AU  - Podolski-Renić, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6483
AB  - Background: Following the traditional Serbian use of cyclamen tubers in the treatment of the most aggressive forms of lung cancer, we performed methanolic extraction of fresh tubers of Cyclamen hederifolium to isolate and identify bioactive constituents. The triterpene saponin deglucocyclamine (SDGC) was identified as a major constituent of cyclamen extract, and its anticancer effects were studied using a panel of NCI-60 cell lines and primary cell cultures obtained from patients with non-small cell lung cancer (NSCLC). Material and Methods: The cyclamen tubers were ground, lyophilized, and extracted with methanol at room temperature with the use of an ultrasonic bath. The part of the methanol extract was further fractionated by dissolving in H2O and then washed with CH2Cl2. The water layer was extracted with n-BuOH. The butanol extract was fractionated by isocratic CC on silica gel with CHCl3−MeOH−H2O eluent. This resulted in the isolation of triterpene (SDGC, C52H84O22) which was identified using 1D and 2D NMR spectra. SDGC was tested at 10 µM against a panel of NCI-60 cancer cell lines and then over a concentration range of 0.01-100 µM using the sulforhodamine B (SRB) assay. SDGC was also tested in the concentration range of 0.01-10 µM against 5 primary patient-derived NSCLC cell cultures (2 stage IB, 2 stage IIA, and 1 stage IIB) using the MTT assay. Cell death analysis was performed in patient-derived NSCLC cells using annexin/propidium iodide staining and flow cytometry. Results: SDGC at 10 µM after 72 h significantly inhibited cell growth of all tested cancer cell lines in the NCI-60 panel. Therefore, SDGC IC50 values were evaluated across the entire NCI-60 panel and ranged from 600 nM to 1 µM. In patient-derived NSCLC cells, SDGC IC50 values were between 1.3 µM and 4.6 µM after 72 h of treatment. SDGC at 10 µM induced late apoptosis and necrosis, significantly reducing the percentage of viable cells to 40% after 48 h. At the same concentration, cisplatin was ineffective against patient-derived NSCLC cells. Conclusions: The triterpene saponin deglucocyclamine (SDGC), whose anticancer effects have not been studied before, showed promising results against NSCLC, melanoma, colon, breast, ovarian, kidney, prostate, and CNS cancer cell lines, as well as patient-derived NSCLC cells. Further more detailed studies of SDGC at the cellular and molecular level are planned.
PB  - Belgrade: Serbian Association for Cancer Research
C3  - Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia
T1  - The anticancer effects of triterpene saponin deglucocyclamine isolated from Cyclamen hederifolium
SP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6483
ER  - 

@conference{
author = "Pajović, Milica and Stanković Jeremić, Jovana and Jovanović Stojanov, Sofija and Gođevac, Dejan and Pešić, Milica and Podolski-Renić, Ana",
year = "2023",
abstract = "Background: Following the traditional Serbian use of cyclamen tubers in the treatment of the most aggressive forms of lung cancer, we performed methanolic extraction of fresh tubers of Cyclamen hederifolium to isolate and identify bioactive constituents. The triterpene saponin deglucocyclamine (SDGC) was identified as a major constituent of cyclamen extract, and its anticancer effects were studied using a panel of NCI-60 cell lines and primary cell cultures obtained from patients with non-small cell lung cancer (NSCLC). Material and Methods: The cyclamen tubers were ground, lyophilized, and extracted with methanol at room temperature with the use of an ultrasonic bath. The part of the methanol extract was further fractionated by dissolving in H2O and then washed with CH2Cl2. The water layer was extracted with n-BuOH. The butanol extract was fractionated by isocratic CC on silica gel with CHCl3−MeOH−H2O eluent. This resulted in the isolation of triterpene (SDGC, C52H84O22) which was identified using 1D and 2D NMR spectra. SDGC was tested at 10 µM against a panel of NCI-60 cancer cell lines and then over a concentration range of 0.01-100 µM using the sulforhodamine B (SRB) assay. SDGC was also tested in the concentration range of 0.01-10 µM against 5 primary patient-derived NSCLC cell cultures (2 stage IB, 2 stage IIA, and 1 stage IIB) using the MTT assay. Cell death analysis was performed in patient-derived NSCLC cells using annexin/propidium iodide staining and flow cytometry. Results: SDGC at 10 µM after 72 h significantly inhibited cell growth of all tested cancer cell lines in the NCI-60 panel. Therefore, SDGC IC50 values were evaluated across the entire NCI-60 panel and ranged from 600 nM to 1 µM. In patient-derived NSCLC cells, SDGC IC50 values were between 1.3 µM and 4.6 µM after 72 h of treatment. SDGC at 10 µM induced late apoptosis and necrosis, significantly reducing the percentage of viable cells to 40% after 48 h. At the same concentration, cisplatin was ineffective against patient-derived NSCLC cells. Conclusions: The triterpene saponin deglucocyclamine (SDGC), whose anticancer effects have not been studied before, showed promising results against NSCLC, melanoma, colon, breast, ovarian, kidney, prostate, and CNS cancer cell lines, as well as patient-derived NSCLC cells. Further more detailed studies of SDGC at the cellular and molecular level are planned.",
publisher = "Belgrade: Serbian Association for Cancer Research",
journal = "Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia",
title = "The anticancer effects of triterpene saponin deglucocyclamine isolated from Cyclamen hederifolium",
pages = "61",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6483"
}

Pajović, M., Stanković Jeremić, J., Jovanović Stojanov, S., Gođevac, D., Pešić, M.,& Podolski-Renić, A.. (2023). The anticancer effects of triterpene saponin deglucocyclamine isolated from Cyclamen hederifolium. in Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia
Belgrade: Serbian Association for Cancer Research., 61.
https://hdl.handle.net/21.15107/rcub_ibiss_6483

Pajović M, Stanković Jeremić J, Jovanović Stojanov S, Gođevac D, Pešić M, Podolski-Renić A. The anticancer effects of triterpene saponin deglucocyclamine isolated from Cyclamen hederifolium. in Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia. 2023;:61.
https://hdl.handle.net/21.15107/rcub_ibiss_6483 .

Pajović, Milica, Stanković Jeremić, Jovana, Jovanović Stojanov, Sofija, Gođevac, Dejan, Pešić, Milica, Podolski-Renić, Ana, "The anticancer effects of triterpene saponin deglucocyclamine isolated from Cyclamen hederifolium" in Proceedings book of The Sixth Congress of The Serbian Association for Cancer Research with international participation: From Collaboration to Innovation in Cancer Research; 2023 Oct 2-4; Belgrade, Serbia (2023):61,
https://hdl.handle.net/21.15107/rcub_ibiss_6483 .

TY  - JOUR
AU  - Zdunić, Gordana
AU  - Alimpić Aradski, Ana
AU  - Gođevac, Dejan
AU  - Živković, Jelena
AU  - Duletić Laušević, Sonja
AU  - Krstić Milošević, Dijana
AU  - Šavikin, Katarina
PY  - 2020
UR  - internal-pdf://Aronija 2020.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3619
AB  - Chokeberries (Aronia melanocarpa (Michx.) Elliot have recently become one of the most popular and widely used berry fruits, and there are numerous studies dealing with the investigation of their chemical composition and biological activities. Unlike berries, chokeberry leaves were far less investigated. Therefore, the aim of this study was to conduct a bioactivity-based analysis of chokeberry leaves collected after berries were harvested. Chemical analysis of hydroethanolic chokeberry extract and its ethyl acetate, n-butanol, and water fractions was performed. Antioxidant, hypoglycemic, and antineurodegenerative activities of the obtained samples were assessed by in vitro tests. The highest total phenolics content was found in n-butanol fraction (221.5 mg gallic acid equivalents/g dw). Fourteen compounds (six phenolic acids and eight flavonoids) were identified in the samples. Caffeoylquinic acid derivatives and quercetin glycosides were dominant phenolic compounds. The highest content of phenolic acids, as well as flavonoids, was recorded in n-butanol fraction (80.75 mg/g dw and 47.79 mg/g dw, respectively). This fraction was the most active in 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assays. Also, the n-butanol fraction was the most effective inhibitor of acetylcholinesterase (AChE) and tyrosinase (TYR) enzymes. All samples showed a better inhibitory effect on α-glucosidase enzyme with inhibition concentrations IC50 = 4.21 - 9.22 μg/mL, than α-amylase enzymes (IC50 = 14.36 - 88.61 μg/mL). Pearson’s correlation between the content of individual phenolics and the results of biological tests was performed. Thus, hyperoside and isoquercitrin showed a significant correlation with DPPH and ABTS tests, caffeoylquinic acids with FRAP test, quercetin 3-O-vicianoside with β-carotene assay, hyperoside and isoquercitrin with α-amylase inhibition activity, and isorhamnetin 3-O-rutinoside with α-glucosidase inhibition assay. Considering the obtained results, chokeberry leaves represent a valuable natural source of phenolic compounds with promising potential for the development of new products with beneficial effects on human health.
T2  - Industrial Crops and Products
T1  - In vitro hypoglycemic, antioxidant and antineurodegenerative activity of chokeberry (Aronia melanocarpa) leaves
VL  - 148
DO  - 10.1016/j.indcrop.2020.112328
SP  - 112328
ER  - 

@article{
author = "Zdunić, Gordana and Alimpić Aradski, Ana and Gođevac, Dejan and Živković, Jelena and Duletić Laušević, Sonja and Krstić Milošević, Dijana and Šavikin, Katarina",
year = "2020",
abstract = "Chokeberries (Aronia melanocarpa (Michx.) Elliot have recently become one of the most popular and widely used berry fruits, and there are numerous studies dealing with the investigation of their chemical composition and biological activities. Unlike berries, chokeberry leaves were far less investigated. Therefore, the aim of this study was to conduct a bioactivity-based analysis of chokeberry leaves collected after berries were harvested. Chemical analysis of hydroethanolic chokeberry extract and its ethyl acetate, n-butanol, and water fractions was performed. Antioxidant, hypoglycemic, and antineurodegenerative activities of the obtained samples were assessed by in vitro tests. The highest total phenolics content was found in n-butanol fraction (221.5 mg gallic acid equivalents/g dw). Fourteen compounds (six phenolic acids and eight flavonoids) were identified in the samples. Caffeoylquinic acid derivatives and quercetin glycosides were dominant phenolic compounds. The highest content of phenolic acids, as well as flavonoids, was recorded in n-butanol fraction (80.75 mg/g dw and 47.79 mg/g dw, respectively). This fraction was the most active in 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assays. Also, the n-butanol fraction was the most effective inhibitor of acetylcholinesterase (AChE) and tyrosinase (TYR) enzymes. All samples showed a better inhibitory effect on α-glucosidase enzyme with inhibition concentrations IC50 = 4.21 - 9.22 μg/mL, than α-amylase enzymes (IC50 = 14.36 - 88.61 μg/mL). Pearson’s correlation between the content of individual phenolics and the results of biological tests was performed. Thus, hyperoside and isoquercitrin showed a significant correlation with DPPH and ABTS tests, caffeoylquinic acids with FRAP test, quercetin 3-O-vicianoside with β-carotene assay, hyperoside and isoquercitrin with α-amylase inhibition activity, and isorhamnetin 3-O-rutinoside with α-glucosidase inhibition assay. Considering the obtained results, chokeberry leaves represent a valuable natural source of phenolic compounds with promising potential for the development of new products with beneficial effects on human health.",
journal = "Industrial Crops and Products",
title = "In vitro hypoglycemic, antioxidant and antineurodegenerative activity of chokeberry (Aronia melanocarpa) leaves",
volume = "148",
doi = "10.1016/j.indcrop.2020.112328",
pages = "112328"
}

Zdunić, G., Alimpić Aradski, A., Gođevac, D., Živković, J., Duletić Laušević, S., Krstić Milošević, D.,& Šavikin, K.. (2020). In vitro hypoglycemic, antioxidant and antineurodegenerative activity of chokeberry (Aronia melanocarpa) leaves. in Industrial Crops and Products, 148, 112328.
https://doi.org/10.1016/j.indcrop.2020.112328

Zdunić G, Alimpić Aradski A, Gođevac D, Živković J, Duletić Laušević S, Krstić Milošević D, Šavikin K. In vitro hypoglycemic, antioxidant and antineurodegenerative activity of chokeberry (Aronia melanocarpa) leaves. in Industrial Crops and Products. 2020;148:112328.
doi:10.1016/j.indcrop.2020.112328 .

Zdunić, Gordana, Alimpić Aradski, Ana, Gođevac, Dejan, Živković, Jelena, Duletić Laušević, Sonja, Krstić Milošević, Dijana, Šavikin, Katarina, "In vitro hypoglycemic, antioxidant and antineurodegenerative activity of chokeberry (Aronia melanocarpa) leaves" in Industrial Crops and Products, 148 (2020):112328,
https://doi.org/10.1016/j.indcrop.2020.112328 . .

TY  - JOUR
AU  - Stanković, Jovana
AU  - Novaković, Miroslav
AU  - Tešević, Vele
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Zdunić, Gordana
AU  - Dajić-Stevanović, Zora
AU  - Gođevac, Dejan
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3613
AB  - This study was performed to determine the main antibacterial com­po­unds of the essential oil (ЕО) of saltmarsh plant Artemisia santonicum (Aster­aceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was deter­mined by 1D- and 2D-NMR and GC–MS techniques. Antibacterial acti­vity of isogeranic acid against all tested bacteria was significantly higher than EO and even than both controls streptomycin and ampicillin. In further inves­tigation of antibiofilm and antiquorum sensing activity EO exhibited the best inhibition of the biofilm formation at 1/8 minimal inhibitory concentration (MIC) and iso­geranic acid at 1/2 MIC. Both EO and isogeranic acid possessed pyocyanin inhibitory activity showing the reduction of pigment at 60.6 and 62.8 %, res­pect­ively, at 1/2 MIC concentrations.
T2  - Journal of the Serbian Chemical Society
T1  - HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil
IS  - 12
VL  - 84
DO  - 10.2298/jsc190513106s
SP  - 1355
EP  - 1365
ER  - 

@article{
author = "Stanković, Jovana and Novaković, Miroslav and Tešević, Vele and Ćirić, Ana and Soković, Marina and Zdunić, Gordana and Dajić-Stevanović, Zora and Gođevac, Dejan",
year = "2019",
abstract = "This study was performed to determine the main antibacterial com­po­unds of the essential oil (ЕО) of saltmarsh plant Artemisia santonicum (Aster­aceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was deter­mined by 1D- and 2D-NMR and GC–MS techniques. Antibacterial acti­vity of isogeranic acid against all tested bacteria was significantly higher than EO and even than both controls streptomycin and ampicillin. In further inves­tigation of antibiofilm and antiquorum sensing activity EO exhibited the best inhibition of the biofilm formation at 1/8 minimal inhibitory concentration (MIC) and iso­geranic acid at 1/2 MIC. Both EO and isogeranic acid possessed pyocyanin inhibitory activity showing the reduction of pigment at 60.6 and 62.8 %, res­pect­ively, at 1/2 MIC concentrations.",
journal = "Journal of the Serbian Chemical Society",
title = "HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil",
number = "12",
volume = "84",
doi = "10.2298/jsc190513106s",
pages = "1355-1365"
}

Stanković, J., Novaković, M., Tešević, V., Ćirić, A., Soković, M., Zdunić, G., Dajić-Stevanović, Z.,& Gođevac, D.. (2019). HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil. in Journal of the Serbian Chemical Society, 84(12), 1355-1365.
https://doi.org/10.2298/jsc190513106s

Stanković J, Novaković M, Tešević V, Ćirić A, Soković M, Zdunić G, Dajić-Stevanović Z, Gođevac D. HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil. in Journal of the Serbian Chemical Society. 2019;84(12):1355-1365.
doi:10.2298/jsc190513106s .

Stanković, Jovana, Novaković, Miroslav, Tešević, Vele, Ćirić, Ana, Soković, Marina, Zdunić, Gordana, Dajić-Stevanović, Zora, Gođevac, Dejan, "HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil" in Journal of the Serbian Chemical Society, 84, no. 12 (2019):1355-1365,
https://doi.org/10.2298/jsc190513106s . .

TY  - JOUR
AU  - Stanković, Jovana
AU  - Gođevac, Dejan
AU  - Tešević, Vele
AU  - Dajić-Stevanović, Zora
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Novaković, Miroslav
PY  - 2019
UR  - http://pubs.acs.org/doi/10.1021/acs.jnatprod.8b00970
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3397
AB  - A new flavonoid glucoside derivative, patuletin 3 -O-(2- O-feruloyl)-β-d-glucuronopyranosyl-(1→2)-β-d-glucopyranoside, named atriplexin IV (1), and three new triterpenoid saponin derivatives, two sulfonylated, β-d-glucopyranosyl-3 -O-(2- O-sulfo-β-d-galactopyranosyl)-(1→2)-α-l-arabinopyranoside-30-alolean-12-en-28-oate (2), named atriplexogenin I, β-d-glucopyranosyl-3- O-(2- O-sulfo-β-d-galactopyranosyl)-(1→2)-α-l-arabinopyranoside)-30-hydroxyolean-12-en-28-oate (3), named atriplexogenin II, and β-d-glucopyranosyl-3 -O-(β-d-glucopyranosyl-(1→2)-β-d-galactopyranosyl-(1→2)-α-l-arabinopyranoside)-30-alolean-12-en-28-oate (4), named atriplexogenin III, were isolated by silica gel column and semipreparative HPLC chromatography from the n-butanol extract of the salt marsh plant Atriplex tatarica. In addition, two known secondary metabolites, patuletin3 -O-β-d-apiofuranosyl-(1‴→2″)-β-d-glucopyranoside (5) and patuletin 3 -O-5‴- O-feruloyl-β-d-apiofuranosyl-(1‴→2″)-β-d-glucopyranoside (6), were isolated for the first time from A. tatarica. The structures of the isolated compounds were elucidated by 1D and 2D NMR, HRESIMS, IR, and UV data. Antibacterial activity by the microdilution method and antibiofilm activity against P. aeruginosa were assessed. Compound 5 possesses significant antibacterial activity, while the most potent antibiofilm agent is compound 2.
PB  - American Chemical Society and American Society of Pharmacognosy
T2  - Journal of Natural Products
T1  - Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa.
IS  - 6
VL  - 82
DO  - 10.1021/acs.jnatprod.8b00970
SP  - 1487
EP  - 1495
ER  - 

@article{
author = "Stanković, Jovana and Gođevac, Dejan and Tešević, Vele and Dajić-Stevanović, Zora and Ćirić, Ana and Soković, Marina and Novaković, Miroslav",
year = "2019",
abstract = "A new flavonoid glucoside derivative, patuletin 3 -O-(2- O-feruloyl)-β-d-glucuronopyranosyl-(1→2)-β-d-glucopyranoside, named atriplexin IV (1), and three new triterpenoid saponin derivatives, two sulfonylated, β-d-glucopyranosyl-3 -O-(2- O-sulfo-β-d-galactopyranosyl)-(1→2)-α-l-arabinopyranoside-30-alolean-12-en-28-oate (2), named atriplexogenin I, β-d-glucopyranosyl-3- O-(2- O-sulfo-β-d-galactopyranosyl)-(1→2)-α-l-arabinopyranoside)-30-hydroxyolean-12-en-28-oate (3), named atriplexogenin II, and β-d-glucopyranosyl-3 -O-(β-d-glucopyranosyl-(1→2)-β-d-galactopyranosyl-(1→2)-α-l-arabinopyranoside)-30-alolean-12-en-28-oate (4), named atriplexogenin III, were isolated by silica gel column and semipreparative HPLC chromatography from the n-butanol extract of the salt marsh plant Atriplex tatarica. In addition, two known secondary metabolites, patuletin3 -O-β-d-apiofuranosyl-(1‴→2″)-β-d-glucopyranoside (5) and patuletin 3 -O-5‴- O-feruloyl-β-d-apiofuranosyl-(1‴→2″)-β-d-glucopyranoside (6), were isolated for the first time from A. tatarica. The structures of the isolated compounds were elucidated by 1D and 2D NMR, HRESIMS, IR, and UV data. Antibacterial activity by the microdilution method and antibiofilm activity against P. aeruginosa were assessed. Compound 5 possesses significant antibacterial activity, while the most potent antibiofilm agent is compound 2.",
publisher = "American Chemical Society and American Society of Pharmacognosy",
journal = "Journal of Natural Products",
title = "Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa.",
number = "6",
volume = "82",
doi = "10.1021/acs.jnatprod.8b00970",
pages = "1487-1495"
}

Stanković, J., Gođevac, D., Tešević, V., Dajić-Stevanović, Z., Ćirić, A., Soković, M.,& Novaković, M.. (2019). Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa.. in Journal of Natural Products
American Chemical Society and American Society of Pharmacognosy., 82(6), 1487-1495.
https://doi.org/10.1021/acs.jnatprod.8b00970

Stanković J, Gođevac D, Tešević V, Dajić-Stevanović Z, Ćirić A, Soković M, Novaković M. Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa.. in Journal of Natural Products. 2019;82(6):1487-1495.
doi:10.1021/acs.jnatprod.8b00970 .

Stanković, Jovana, Gođevac, Dejan, Tešević, Vele, Dajić-Stevanović, Zora, Ćirić, Ana, Soković, Marina, Novaković, Miroslav, "Antibacterial and Antibiofilm Activity of Flavonoid and Saponin Derivatives from Atriplex tatarica against Pseudomonas aeruginosa." in Journal of Natural Products, 82, no. 6 (2019):1487-1495,
https://doi.org/10.1021/acs.jnatprod.8b00970 . .

TY  - JOUR
AU  - Stević, Tatjana
AU  - Stanković, Slaviša
AU  - Šavikin, Katarina
AU  - Gođevac, Dejan
AU  - Dimkić, Ivica
AU  - Soković, Marina
AU  - Berić, Tanja
PY  - 2014
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/412
AB  - In recent years great attention was paid to biological control, the application of natural products in order to protect crops and medicinal plants against contamination with phytopathogenic and saprophytic fungi. Essential oils have been evaluated as a potentially safe replacement for chemicals used for that purpose. In this regard, antifungal potential of six essential oils was examined coupled with determination of the complete qualitative and quantitative chemical composition by GC-FID and GC-MS analysis. While essential oils of rosemary, sage, Spanish sage and black pepper were exclusively composed of monoterpenes, vetiver essential oil was entirely composed of sesquiterpenes. In essential oil of cinnamon dominates (E)-cinnamaldehyde. Applying the in vitro microdilution method, it was found that all essential oils were active in inhibiting the growth of all tested 21 pre- and post-harvest phytopathogenic and saprophytic fungi. MIC and MFC ranged from 1.2 mg ml-1 up to 22.6 mg ml-1 according to the test oil. Rosemary oil showed the best antifungal potential, followed by black pepper and cinnamon oil. Sage and Spanish sage oils also exhibited significant antifungal potential. Vetiver oil demonstrated the lowest antifungal activity. Essential oils that showed considerable antifungal potential are good candidates for further examination of their use in preventing and/or protection of medicinal plants, their seeds and dried drugs against fungal infections, both in the field and in warehouses.
AB  - Poslednjih godina velika pažnja poklanja se biološkoj kontroli to jest primeni prirodnih produkata u zaštiti, kako poljoprivrednih kultura, tako i lekovitog bilja od kontaminacije fitopatogenim i saprofitnim gljivama. U tom smislu, ispitivan je antifungalni potencijal šest etarskih ulja uz određivanje kompletnog kvalitativnog i kvantitativnog hemijskog sastava primenom GC-FID i GC-MS analiza. Dok se korišćeni uzorci etarskih ulja ruzmarina, žalfije, španske žalfije i crnog bibera isključivo sastoje od monoterpena, u ulju vetivera potpuno dominiraju seskviterpeni. U etarskom ulju cimeta najzastupljenija komponneta je bila (E)- cinemaldehid. Primenom in vitro mikrodilucione metode, utvrđeno je da su sva ulja bila aktivna u inhibiciji rasta 21 testirane, pre- i post-žetvene, fitopatogene i saprofitne gljive, sa razlikama u efikasnosti. MIC i MFC vrednosti su varirale u opsegu od 1,2 mg ml-1 do 22,6 mg ml-1, u zavisnosti od ispitivanog ulja. Najbolja antifungalna aktivnost utvrđena je za ulje ruzmarina, nešto slabija za ulja crnog bibera i cimeta, dok je ulje vetivera ispoljilo najslabiju aktivnost. Etarska ulja obe vrste žalfija ispoljila su značajan antifungalni potencijal. Etarska ulja koja poseduju značajan antifungalni potencijal su dobri kandidati za nastavak istraživanja u pravcu njihove primene u prevenciji i zaštiti useva lekovitog bilja, kao i njihovih semena i osušenih biljnih droga od gljivičnih infekcija, i u polju i u skladištima.
T2  - Lekovite sirovine
T1  - Hemijski sastav i inhibitorna aktivnost odabranih etarskih ulja na gljive izolovane sa lekovitog bilja
T1  - Chemical composition and inhibitory activity of selected essential oils against fungi isolated from medicinal plants
IS  - 34
VL  - 34
SP  - 69
EP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_412
ER  - 

@article{
author = "Stević, Tatjana and Stanković, Slaviša and Šavikin, Katarina and Gođevac, Dejan and Dimkić, Ivica and Soković, Marina and Berić, Tanja",
year = "2014, 2014",
abstract = "In recent years great attention was paid to biological control, the application of natural products in order to protect crops and medicinal plants against contamination with phytopathogenic and saprophytic fungi. Essential oils have been evaluated as a potentially safe replacement for chemicals used for that purpose. In this regard, antifungal potential of six essential oils was examined coupled with determination of the complete qualitative and quantitative chemical composition by GC-FID and GC-MS analysis. While essential oils of rosemary, sage, Spanish sage and black pepper were exclusively composed of monoterpenes, vetiver essential oil was entirely composed of sesquiterpenes. In essential oil of cinnamon dominates (E)-cinnamaldehyde. Applying the in vitro microdilution method, it was found that all essential oils were active in inhibiting the growth of all tested 21 pre- and post-harvest phytopathogenic and saprophytic fungi. MIC and MFC ranged from 1.2 mg ml-1 up to 22.6 mg ml-1 according to the test oil. Rosemary oil showed the best antifungal potential, followed by black pepper and cinnamon oil. Sage and Spanish sage oils also exhibited significant antifungal potential. Vetiver oil demonstrated the lowest antifungal activity. Essential oils that showed considerable antifungal potential are good candidates for further examination of their use in preventing and/or protection of medicinal plants, their seeds and dried drugs against fungal infections, both in the field and in warehouses., Poslednjih godina velika pažnja poklanja se biološkoj kontroli to jest primeni prirodnih produkata u zaštiti, kako poljoprivrednih kultura, tako i lekovitog bilja od kontaminacije fitopatogenim i saprofitnim gljivama. U tom smislu, ispitivan je antifungalni potencijal šest etarskih ulja uz određivanje kompletnog kvalitativnog i kvantitativnog hemijskog sastava primenom GC-FID i GC-MS analiza. Dok se korišćeni uzorci etarskih ulja ruzmarina, žalfije, španske žalfije i crnog bibera isključivo sastoje od monoterpena, u ulju vetivera potpuno dominiraju seskviterpeni. U etarskom ulju cimeta najzastupljenija komponneta je bila (E)- cinemaldehid. Primenom in vitro mikrodilucione metode, utvrđeno je da su sva ulja bila aktivna u inhibiciji rasta 21 testirane, pre- i post-žetvene, fitopatogene i saprofitne gljive, sa razlikama u efikasnosti. MIC i MFC vrednosti su varirale u opsegu od 1,2 mg ml-1 do 22,6 mg ml-1, u zavisnosti od ispitivanog ulja. Najbolja antifungalna aktivnost utvrđena je za ulje ruzmarina, nešto slabija za ulja crnog bibera i cimeta, dok je ulje vetivera ispoljilo najslabiju aktivnost. Etarska ulja obe vrste žalfija ispoljila su značajan antifungalni potencijal. Etarska ulja koja poseduju značajan antifungalni potencijal su dobri kandidati za nastavak istraživanja u pravcu njihove primene u prevenciji i zaštiti useva lekovitog bilja, kao i njihovih semena i osušenih biljnih droga od gljivičnih infekcija, i u polju i u skladištima.",
journal = "Lekovite sirovine",
title = "Hemijski sastav i inhibitorna aktivnost odabranih etarskih ulja na gljive izolovane sa lekovitog bilja, Chemical composition and inhibitory activity of selected essential oils against fungi isolated from medicinal plants",
number = "34",
volume = "34",
pages = "69-80",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_412"
}

Stević, T., Stanković, S., Šavikin, K., Gođevac, D., Dimkić, I., Soković, M.,& Berić, T.. (2014). Hemijski sastav i inhibitorna aktivnost odabranih etarskih ulja na gljive izolovane sa lekovitog bilja. in Lekovite sirovine, 34(34), 69-80.
https://hdl.handle.net/21.15107/rcub_ibiss_412

Stević T, Stanković S, Šavikin K, Gođevac D, Dimkić I, Soković M, Berić T. Hemijski sastav i inhibitorna aktivnost odabranih etarskih ulja na gljive izolovane sa lekovitog bilja. in Lekovite sirovine. 2014;34(34):69-80.
https://hdl.handle.net/21.15107/rcub_ibiss_412 .

Stević, Tatjana, Stanković, Slaviša, Šavikin, Katarina, Gođevac, Dejan, Dimkić, Ivica, Soković, Marina, Berić, Tanja, "Hemijski sastav i inhibitorna aktivnost odabranih etarskih ulja na gljive izolovane sa lekovitog bilja" in Lekovite sirovine, 34, no. 34 (2014):69-80,
https://hdl.handle.net/21.15107/rcub_ibiss_412 .

TY  - JOUR
AU  - Gođevac, Dejan
AU  - Soković, Marina
AU  - Vujisić, Ljubodrag
AU  - Vučković, Ivan
AU  - Vajs, Vlatka
AU  - Marin, Petar D.
AU  - Tešević, Vele
PY  - 2012
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/564
AB  - An investigation of the chemical composition and antimicrobial activity of the essential oil of Galatella linosyris is presented. The chemical analysis (GC/MS, NMR) showed that sabinene (40 %), β-pinene (35.5 %), α-pinene (4.5 %), limonene (4 %), γ-muurolene (4 %), and (E)-caryophyllene (3.3 %) were dominant components in this oil. Microdilution assays were used to evaluate the minimum inhibitory concentration (MIC) and the minimum bactericidal/ fungicidal concentrations (MBC/MFC). G. linosyris essential oil exhibited better antibacterial activity against some of the tested bacteria than antifungal activity.
AB  - U ovom radu je prikazano ispitivanje hemijskog sastava i antimikrobne aktivnosti etarskog ulja biljne vrste Galatella linosyris. Hemijskom analizom (GC-MS i NMR) je utvrđeno da su glavni sastojci ovog etarskog ulja sabinen (40 %), β-pinen (35,5 %) , α-pinen (4,5 %), limonen (4 %), γ-muurolen (4 %) i (E)-kariofilen (3,3 %). Za procenu minimalne inhibitorne koncentracije (MIC) i minimalne baktericidne/fungicidne koncentracije (MBC/ /MFC) korišćeni su testovi mikrorazblaženja. Etarsko ulje G. linosyris je pokazalo bolju antibakterijsku nego antifungalnu aktivnost.
T2  - Journal of the Serbian Chemical Society
T1  - Sastav i antimikrobna aktivnost etarskog ulja iz Galatella linosyris (L.) Rchb. f. (Asteraceae)
T1  - Composition and antimicrobial activity of the essential oil from Galatella linosyris (L.) Rchb. f. (Asteraceae)
IS  - 5
VL  - 77
SP  - 619
EP  - 626
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_564
ER  - 

@article{
author = "Gođevac, Dejan and Soković, Marina and Vujisić, Ljubodrag and Vučković, Ivan and Vajs, Vlatka and Marin, Petar D. and Tešević, Vele",
year = "2012, 2012",
abstract = "An investigation of the chemical composition and antimicrobial activity of the essential oil of Galatella linosyris is presented. The chemical analysis (GC/MS, NMR) showed that sabinene (40 %), β-pinene (35.5 %), α-pinene (4.5 %), limonene (4 %), γ-muurolene (4 %), and (E)-caryophyllene (3.3 %) were dominant components in this oil. Microdilution assays were used to evaluate the minimum inhibitory concentration (MIC) and the minimum bactericidal/ fungicidal concentrations (MBC/MFC). G. linosyris essential oil exhibited better antibacterial activity against some of the tested bacteria than antifungal activity., U ovom radu je prikazano ispitivanje hemijskog sastava i antimikrobne aktivnosti etarskog ulja biljne vrste Galatella linosyris. Hemijskom analizom (GC-MS i NMR) je utvrđeno da su glavni sastojci ovog etarskog ulja sabinen (40 %), β-pinen (35,5 %) , α-pinen (4,5 %), limonen (4 %), γ-muurolen (4 %) i (E)-kariofilen (3,3 %). Za procenu minimalne inhibitorne koncentracije (MIC) i minimalne baktericidne/fungicidne koncentracije (MBC/ /MFC) korišćeni su testovi mikrorazblaženja. Etarsko ulje G. linosyris je pokazalo bolju antibakterijsku nego antifungalnu aktivnost.",
journal = "Journal of the Serbian Chemical Society",
title = "Sastav i antimikrobna aktivnost etarskog ulja iz Galatella linosyris (L.) Rchb. f. (Asteraceae), Composition and antimicrobial activity of the essential oil from Galatella linosyris (L.) Rchb. f. (Asteraceae)",
number = "5",
volume = "77",
pages = "619-626",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_564"
}

Gođevac, D., Soković, M., Vujisić, L., Vučković, I., Vajs, V., Marin, P. D.,& Tešević, V.. (2012). Sastav i antimikrobna aktivnost etarskog ulja iz Galatella linosyris (L.) Rchb. f. (Asteraceae). in Journal of the Serbian Chemical Society, 77(5), 619-626.
https://hdl.handle.net/21.15107/rcub_ibiss_564

Gođevac D, Soković M, Vujisić L, Vučković I, Vajs V, Marin PD, Tešević V. Sastav i antimikrobna aktivnost etarskog ulja iz Galatella linosyris (L.) Rchb. f. (Asteraceae). in Journal of the Serbian Chemical Society. 2012;77(5):619-626.
https://hdl.handle.net/21.15107/rcub_ibiss_564 .

Gođevac, Dejan, Soković, Marina, Vujisić, Ljubodrag, Vučković, Ivan, Vajs, Vlatka, Marin, Petar D., Tešević, Vele, "Sastav i antimikrobna aktivnost etarskog ulja iz Galatella linosyris (L.) Rchb. f. (Asteraceae)" in Journal of the Serbian Chemical Society, 77, no. 5 (2012):619-626,
https://hdl.handle.net/21.15107/rcub_ibiss_564 .