TY  - JOUR
AU  - Jadranin, Milka
AU  - Savić, Danica
AU  - Lupšić, Ema
AU  - Podolski-Renić, Ana
AU  - Pešić, Milica
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
AU  - Krstić, Gordana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6557
AB  - Euphorbia seguieriana ssp. seguieriana Necker (ES) and Euphorbia cyparissias (EC) with a habitat in the Deliblato Sands were the subject of this examination. The latexes of these so far insufficiently investigated species of the Euphorbia genus are used in traditional medicine for the treatment of wounds and warts on the skin. To determine their chemical composition, non-targeted screening of the latexes' chloroform extracts was performed using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry employing an electrospray ionization source (LC-ESI QTOF MS). The analysis of the obtained results showed that the latexes of ES and EC represent rich sources of diterpenes, tentatively identified as jatrophanes, ingenanes, tiglianes, myrsinanes, premyrsinanes, and others. Examination of the anticancer activity of the ES and EC latex extracts showed that both extracts significantly inhibited the growth of the non-small cell lung carcinoma NCI-H460 and glioblastoma U87 cell lines as well as of their corresponding multi-drug resistant (MDR) cell lines, NCI-H460/R and U87-TxR. The obtained results also revealed that the ES and EC extracts inhibited the function of P-glycoprotein (P-gp) in MDR cancer cells, whose overexpression is one of the main mechanisms underlying MDR.
PB  - Basel: MDPI
T2  - Plants
T1  - LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity
IS  - 24
VL  - 12
DO  - 10.3390/plants12244181
SP  - 4181
ER  - 

@article{
author = "Jadranin, Milka and Savić, Danica and Lupšić, Ema and Podolski-Renić, Ana and Pešić, Milica and Tešević, Vele and Milosavljević, Slobodan and Krstić, Gordana",
year = "2023",
abstract = "Euphorbia seguieriana ssp. seguieriana Necker (ES) and Euphorbia cyparissias (EC) with a habitat in the Deliblato Sands were the subject of this examination. The latexes of these so far insufficiently investigated species of the Euphorbia genus are used in traditional medicine for the treatment of wounds and warts on the skin. To determine their chemical composition, non-targeted screening of the latexes' chloroform extracts was performed using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry employing an electrospray ionization source (LC-ESI QTOF MS). The analysis of the obtained results showed that the latexes of ES and EC represent rich sources of diterpenes, tentatively identified as jatrophanes, ingenanes, tiglianes, myrsinanes, premyrsinanes, and others. Examination of the anticancer activity of the ES and EC latex extracts showed that both extracts significantly inhibited the growth of the non-small cell lung carcinoma NCI-H460 and glioblastoma U87 cell lines as well as of their corresponding multi-drug resistant (MDR) cell lines, NCI-H460/R and U87-TxR. The obtained results also revealed that the ES and EC extracts inhibited the function of P-glycoprotein (P-gp) in MDR cancer cells, whose overexpression is one of the main mechanisms underlying MDR.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity",
number = "24",
volume = "12",
doi = "10.3390/plants12244181",
pages = "4181"
}

Jadranin, M., Savić, D., Lupšić, E., Podolski-Renić, A., Pešić, M., Tešević, V., Milosavljević, S.,& Krstić, G.. (2023). LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity. in Plants
Basel: MDPI., 12(24), 4181.
https://doi.org/10.3390/plants12244181

Jadranin M, Savić D, Lupšić E, Podolski-Renić A, Pešić M, Tešević V, Milosavljević S, Krstić G. LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity. in Plants. 2023;12(24):4181.
doi:10.3390/plants12244181 .

Jadranin, Milka, Savić, Danica, Lupšić, Ema, Podolski-Renić, Ana, Pešić, Milica, Tešević, Vele, Milosavljević, Slobodan, Krstić, Gordana, "LC-ESI QToF MS Non-Targeted Screening of Latex Extracts of Euphorbia seguieriana ssp. seguieriana Necker and Euphorbia cyparissias and Determination of Their Potential Anticancer Activity" in Plants, 12, no. 24 (2023):4181,
https://doi.org/10.3390/plants12244181 . .

TY  - CONF
AU  - Krstić, Gordana
AU  - Jadranin, Milka
AU  - Jovanović Stojanov, Sofija
AU  - Pešić, Milica
AU  - Tešević, Vele
AU  - Milosavljević, Slobodan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6594
AB  - In this research, from two species, E. palustris and E. lucida, four ingenane derivatives were isolated. Their anticancer effects were evaluated in the human melanoma – 518A2 cell line and compared with the effects of ingenolmebutate. Selectivity towards human melanoma cells was determined using normal human keratinocytes – HaCaT.
PB  - Skopje: Macedonian Pharmaceutical Association
C3  - 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia
T1  - Anti-melanoma effects of ingenanes isolated from Euphorbia species
DO  - 10.33320/maced.pharm.bull.2022.68.04.006
SP  - 23
EP  - 24
ER  - 

@conference{
author = "Krstić, Gordana and Jadranin, Milka and Jovanović Stojanov, Sofija and Pešić, Milica and Tešević, Vele and Milosavljević, Slobodan",
year = "2022",
abstract = "In this research, from two species, E. palustris and E. lucida, four ingenane derivatives were isolated. Their anticancer effects were evaluated in the human melanoma – 518A2 cell line and compared with the effects of ingenolmebutate. Selectivity towards human melanoma cells was determined using normal human keratinocytes – HaCaT.",
publisher = "Skopje: Macedonian Pharmaceutical Association",
journal = "11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia",
title = "Anti-melanoma effects of ingenanes isolated from Euphorbia species",
doi = "10.33320/maced.pharm.bull.2022.68.04.006",
pages = "23-24"
}

Krstić, G., Jadranin, M., Jovanović Stojanov, S., Pešić, M., Tešević, V.,& Milosavljević, S.. (2022). Anti-melanoma effects of ingenanes isolated from Euphorbia species. in 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia
Skopje: Macedonian Pharmaceutical Association., 23-24.
https://doi.org/10.33320/maced.pharm.bull.2022.68.04.006

Krstić G, Jadranin M, Jovanović Stojanov S, Pešić M, Tešević V, Milosavljević S. Anti-melanoma effects of ingenanes isolated from Euphorbia species. in 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia. 2022;:23-24.
doi:10.33320/maced.pharm.bull.2022.68.04.006 .

Krstić, Gordana, Jadranin, Milka, Jovanović Stojanov, Sofija, Pešić, Milica, Tešević, Vele, Milosavljević, Slobodan, "Anti-melanoma effects of ingenanes isolated from Euphorbia species" in 11th Conference on Medicinal and Aromatic Plants of Southeast European Countries 2022: CMAPSEEC 2022; 2022 Oct 6-10; Ohrid, Republic of North Macedonia (2022):23-24,
https://doi.org/10.33320/maced.pharm.bull.2022.68.04.006 . .

TY  - CONF
AU  - Marković, Marija
AU  - Skorić, Marijana
AU  - Živković, Suzana
AU  - Jadranin, Milka
AU  - Dragićević, Milan
AU  - Tešević, Vele
AU  - Gligorijević, Nevenka
AU  - Janković, Radmila
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Todorović, Slađana
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6184
AB  - Inula oculus-christi L. is a perennial species distributed in Asia, Central Europe and the Balkan Peninsula. In this study a potential cytotoxic and antimicrobial activity of ethanol extracts obtained from different parts of I. oculus-christi plants was investigated. Cytotoxic activity of extracts was determined on three tumor cell lines (HeLa, FemX and LS-174) by MTT assay. The extracts of leaves and flowers showed significant cytotoxic activity against all investigated cell lines, while rhizome, root and trunk extracts had no cytotoxic activity. The IC50 values for flower extract were 68.70 ± 2.79 μg mL-1 for HeLa cells, 88.91 ± 1.66 μg mL-1 for FemX cells and 81.46 ± 8.45 μg mL-1 for LS-174 cells, whereas the IC50 values for leaf extract were 73.69 ± 5.35 μg mL-1 for He-La cells, 71.58 ± 4.25 μg mL-1 for FemX cells and 96.37 ± 4.92 μg mL-1 for LS-174 cells. The observed cytotoxic activity could be attributed to several bioactive sesquiterpenoids and flavonoids which were more abundant in leaf and flower extracts according to LC-DAD/MS non-targeted screening. In the antimicrobial bioassays against eight bacteria and microfungi, all tested extracts exhibited higher antimicrobial activity in comparison with commercial antimicrobial agents used as control. These results indicate the possibility of application of I. oculus-christi extracts as valuable natural products in medicine, agronomy and food industry.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Cytotoxic and antimicrobial activities of different plant part extracts of Inula oculus-christi
SP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6184
ER  - 

@conference{
author = "Marković, Marija and Skorić, Marijana and Živković, Suzana and Jadranin, Milka and Dragićević, Milan and Tešević, Vele and Gligorijević, Nevenka and Janković, Radmila and Glamočlija, Jasmina and Soković, Marina and Todorović, Slađana",
year = "2015",
abstract = "Inula oculus-christi L. is a perennial species distributed in Asia, Central Europe and the Balkan Peninsula. In this study a potential cytotoxic and antimicrobial activity of ethanol extracts obtained from different parts of I. oculus-christi plants was investigated. Cytotoxic activity of extracts was determined on three tumor cell lines (HeLa, FemX and LS-174) by MTT assay. The extracts of leaves and flowers showed significant cytotoxic activity against all investigated cell lines, while rhizome, root and trunk extracts had no cytotoxic activity. The IC50 values for flower extract were 68.70 ± 2.79 μg mL-1 for HeLa cells, 88.91 ± 1.66 μg mL-1 for FemX cells and 81.46 ± 8.45 μg mL-1 for LS-174 cells, whereas the IC50 values for leaf extract were 73.69 ± 5.35 μg mL-1 for He-La cells, 71.58 ± 4.25 μg mL-1 for FemX cells and 96.37 ± 4.92 μg mL-1 for LS-174 cells. The observed cytotoxic activity could be attributed to several bioactive sesquiterpenoids and flavonoids which were more abundant in leaf and flower extracts according to LC-DAD/MS non-targeted screening. In the antimicrobial bioassays against eight bacteria and microfungi, all tested extracts exhibited higher antimicrobial activity in comparison with commercial antimicrobial agents used as control. These results indicate the possibility of application of I. oculus-christi extracts as valuable natural products in medicine, agronomy and food industry.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Cytotoxic and antimicrobial activities of different plant part extracts of Inula oculus-christi",
pages = "85",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6184"
}

Marković, M., Skorić, M., Živković, S., Jadranin, M., Dragićević, M., Tešević, V., Gligorijević, N., Janković, R., Glamočlija, J., Soković, M.,& Todorović, S.. (2015). Cytotoxic and antimicrobial activities of different plant part extracts of Inula oculus-christi. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society., 85.
https://hdl.handle.net/21.15107/rcub_ibiss_6184

Marković M, Skorić M, Živković S, Jadranin M, Dragićević M, Tešević V, Gligorijević N, Janković R, Glamočlija J, Soković M, Todorović S. Cytotoxic and antimicrobial activities of different plant part extracts of Inula oculus-christi. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:85.
https://hdl.handle.net/21.15107/rcub_ibiss_6184 .

Marković, Marija, Skorić, Marijana, Živković, Suzana, Jadranin, Milka, Dragićević, Milan, Tešević, Vele, Gligorijević, Nevenka, Janković, Radmila, Glamočlija, Jasmina, Soković, Marina, Todorović, Slađana, "Cytotoxic and antimicrobial activities of different plant part extracts of Inula oculus-christi" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):85,
https://hdl.handle.net/21.15107/rcub_ibiss_6184 .