@article{
author = "Mihailović, Vladimir and Mišić, Danijela and Matić, Sanja and Mihailović, Mirjana and Stanić, Snežana and Vrvić, Miroslav M. and Katanić, Jelena and Mladenović, Milan and Stanković, Nevena and Boroja, Tatjana and Stanković, Milan S.",
year = "2015",
abstract = "The purpose of this study was to evaluate the antioxidant potential of
methanol extracts of Gentiana crudata L. aerial parts and roots, as well
as the stability of the phenolic compounds and antioxidant capacity of
extracts during heating, at different pHs and after an in vitro
digestion procedure. Also, their genotoxicity and antigenotoxicity
against carbon tetrachloride in the liver of albino Wistar rats using
the comet assay were evaluated. Three secoiridoid glycosides
(swertiamarin, gentiopicrin, and sweroside) and four phenolic compounds
(orientin, vitexin and two isovitexin-glucosides) were identified as the
major constituents in aerial parts and roots of G. cruciata, using
UHPLC-DAD/+/- HESI-MS/MS analysis. The results of antioxidant assays
showed that aerial parts displayed higher antioxidant activity compared
to the roots, which could be related to higher phenolics content,
especially flavonoids. In general, extracts showed pH and thermal
stability, while duodenal condition had more influence on total phenolic
condition and antioxidant activity of extracts. Both extracts showed a
protective effect against CCl4 in comet assays. The roots extract showed
no genotoxic activity, while aerial parts extract showed slight
genotoxicity at concentrations of 400 mg/kg b.w. (C) 2015 Elsevier B.V.
All rights reserved.",
publisher = "Elsevier",
journal = "Industrial Crops and Products",
title = "Comparative phytochemical analysis of Gentiana cruciata L. roots and
aerial parts, and their biological activities",
volume = "73",
doi = "10.1016/j.indcrop.2015.04.013",
pages = "49-62"
}