TY  - CONF
AU  - Puač, Nevena
AU  - Jovanović, Olivera
AU  - Petrović, Anđelija
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Malović, Gordana
AU  - Škoro, Nikola
PY  - 2022
UR  - https://meetings.aps.org/Meeting/GEC22/Session/EF3.1
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6323
AB  - The atmospheric pressure plasmas (APPs) are known to be rich in Reactive Oxygen and Nitrogen Species (RONS) and this rich chemistry is responsible for triggering of cell mechanisms in case of plant or human/animal cells. We can divide this influence in two groups: (1) RONS in gas phase; (2) RONS in liquid phase. Therefore, in order to better understand the reasons for triggered mechanisms and outcomes (better germination percentage and speed, breakout of dormancy, creation of embryos etc.) we need to know and be able to tailor the plasma chemistry both in gas and liquid phase. We have used several APPs for gas phase treatments of plant cells and also for production of Plasma Activated Water (PAW) in order to investigate the influence of liquid RONS chemistry on plant cells. Here we will present different APP sources that are used for production of PAW used for seed imbibition in germination process and direct treatment of meristematic plant cells. Dielectric Barrier Discharge type of APPJ was used for production of PAW, while the plasma needle type was used for direct treatment of meristematic plant cells. The main idea was to check if plasma treatments can be used as for breaking of dormancy and trigger mechanisms in cells even in a normally non-permissive conditions.
PB  - American Physical Society
C3  - The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan
T1  - Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells
SP  - EF3.00001
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6323
ER  - 

@conference{
author = "Puač, Nevena and Jovanović, Olivera and Petrović, Anđelija and Živković, Suzana and Milutinović, Milica and Malović, Gordana and Škoro, Nikola",
year = "2022",
abstract = "The atmospheric pressure plasmas (APPs) are known to be rich in Reactive Oxygen and Nitrogen Species (RONS) and this rich chemistry is responsible for triggering of cell mechanisms in case of plant or human/animal cells. We can divide this influence in two groups: (1) RONS in gas phase; (2) RONS in liquid phase. Therefore, in order to better understand the reasons for triggered mechanisms and outcomes (better germination percentage and speed, breakout of dormancy, creation of embryos etc.) we need to know and be able to tailor the plasma chemistry both in gas and liquid phase. We have used several APPs for gas phase treatments of plant cells and also for production of Plasma Activated Water (PAW) in order to investigate the influence of liquid RONS chemistry on plant cells. Here we will present different APP sources that are used for production of PAW used for seed imbibition in germination process and direct treatment of meristematic plant cells. Dielectric Barrier Discharge type of APPJ was used for production of PAW, while the plasma needle type was used for direct treatment of meristematic plant cells. The main idea was to check if plasma treatments can be used as for breaking of dormancy and trigger mechanisms in cells even in a normally non-permissive conditions.",
publisher = "American Physical Society",
journal = "The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan",
title = "Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells",
pages = "EF3.00001",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6323"
}

Puač, N., Jovanović, O., Petrović, A., Živković, S., Milutinović, M., Malović, G.,& Škoro, N.. (2022). Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells. in The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan
American Physical Society., EF3.00001.
https://hdl.handle.net/21.15107/rcub_ibiss_6323

Puač N, Jovanović O, Petrović A, Živković S, Milutinović M, Malović G, Škoro N. Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells. in The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan. 2022;:EF3.00001.
https://hdl.handle.net/21.15107/rcub_ibiss_6323 .

Puač, Nevena, Jovanović, Olivera, Petrović, Anđelija, Živković, Suzana, Milutinović, Milica, Malović, Gordana, Škoro, Nikola, "Role of atmospheric pressure plasma in triggering of cell mechanisms in plant cells" in The 75th Annual Gaseous Electronics Conference (GEC); 2011 Oct 3-7; Sendai, Japan (2022):EF3.00001,
https://hdl.handle.net/21.15107/rcub_ibiss_6323 .

TY  - CONF
AU  - Puač, Nevena
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Jovanović, Olivera
AU  - Petrović, Anđelija
AU  - Malović, Gordana
AU  - Škoro, Nikola
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5271
AB  - The population growth together with constant climate changes represent a serious challenge for humankind. Additionally, the usage of the pesticides have created adverse effect on environment,
which in return impact even more agricultural production. In order to comply with the demands and
to adapt to the new conditions the farmers need to change or upgrade existing practices by employing
new technologies. As being a promising tools in application in medicine, non-thermal (cold) plasmas
(NTPs) are seen as a green alternative to conventional fertilizers in agriculture to improve yields,
increase size and robustness of plants and to reduce (or eliminate) the need for pesticides [1, 2].
NTPs have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are formed in gas
phase and, in case of water treatment, in gas/liquid interface in liquid [2, 3]. We can use NTPs in
direct treatments of seeds or plant cells where samples are in contact with plasma gas phase chemistry
or indirectly when treated water is applied to the biological samples. In both cases RONS (short or
long-living) are responsible for triggering various mechanisms and effects in plant cells. To better
understand the reasons for triggered mechanisms and outcomes (better germination percentage and
speed, breakout of dormancy, creation of embryos etc.) it is important to characterize the plasma
chemistry both in gas and liquid phase. We have used several atmospheric pressure plasma systems
in treatments of seeds and plant cells in order to investigate the mechanisms responsible for better
germination in seeds, enzyme response and formation of somatic embryos in non-permissive
conditions. The mechanisms investigated were linked with the chemistry of RONS created in gas
phase and/or deposited in liquid phase. Thus, we were able to acquire the data that can be used in
optimization of plasma treatment processes.
C3  - Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands
T1  - Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions
SP  - 16
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5271
ER  - 

@conference{
author = "Puač, Nevena and Živković, Suzana and Milutinović, Milica and Jovanović, Olivera and Petrović, Anđelija and Malović, Gordana and Škoro, Nikola",
year = "2022",
abstract = "The population growth together with constant climate changes represent a serious challenge for humankind. Additionally, the usage of the pesticides have created adverse effect on environment,
which in return impact even more agricultural production. In order to comply with the demands and
to adapt to the new conditions the farmers need to change or upgrade existing practices by employing
new technologies. As being a promising tools in application in medicine, non-thermal (cold) plasmas
(NTPs) are seen as a green alternative to conventional fertilizers in agriculture to improve yields,
increase size and robustness of plants and to reduce (or eliminate) the need for pesticides [1, 2].
NTPs have rich chemistry of Reactive Oxygen and Nitrogen Species (RONS) that are formed in gas
phase and, in case of water treatment, in gas/liquid interface in liquid [2, 3]. We can use NTPs in
direct treatments of seeds or plant cells where samples are in contact with plasma gas phase chemistry
or indirectly when treated water is applied to the biological samples. In both cases RONS (short or
long-living) are responsible for triggering various mechanisms and effects in plant cells. To better
understand the reasons for triggered mechanisms and outcomes (better germination percentage and
speed, breakout of dormancy, creation of embryos etc.) it is important to characterize the plasma
chemistry both in gas and liquid phase. We have used several atmospheric pressure plasma systems
in treatments of seeds and plant cells in order to investigate the mechanisms responsible for better
germination in seeds, enzyme response and formation of somatic embryos in non-permissive
conditions. The mechanisms investigated were linked with the chemistry of RONS created in gas
phase and/or deposited in liquid phase. Thus, we were able to acquire the data that can be used in
optimization of plasma treatment processes.",
journal = "Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands",
title = "Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions",
pages = "16",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5271"
}

Puač, N., Živković, S., Milutinović, M., Jovanović, O., Petrović, A., Malović, G.,& Škoro, N.. (2022). Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions. in Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands, 16.
https://hdl.handle.net/21.15107/rcub_ibiss_5271

Puač N, Živković S, Milutinović M, Jovanović O, Petrović A, Malović G, Škoro N. Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions. in Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands. 2022;:16.
https://hdl.handle.net/21.15107/rcub_ibiss_5271 .

Puač, Nevena, Živković, Suzana, Milutinović, Milica, Jovanović, Olivera, Petrović, Anđelija, Malović, Gordana, Škoro, Nikola, "Plasma treatment of seeds and plant cells: role of reactive oxygen and nitrogen species in formation of plantlets and embryos in non-permissive conditions" in Book of Abstracts: 9th International Conference on Plasma Medicine (ICPM9);  2022 Jun 27 - Jul 1; Utrecht, The Netherlands (2022):16,
https://hdl.handle.net/21.15107/rcub_ibiss_5271 .

TY  - CONF
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Maletić, Dejan
AU  - Živković, Suzana
AU  - Selaković, Nenad
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj
PY  - 2019
UR  - http://neon.dpp.fmph.uniba.sk/sappxxii/download/SAPP_XXII_JSPP_XI_Book_of_Contributed_Papers.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6199
AB  - Atmospheric pressure plasmas (APPs) have been intensively studied in the last decade due to their high
potential in the applications in medicine, biology and, lately, in agriculture. They are generated in plasma sources of various geometries and configurations and with different electrode material in order to make them efficient for large variety of applications. The types of sources used are mainly atmospheric pressure plasma jets (APPJs), but since some applications require large areas to be treated plan parallel dielectric barrier discharges [3-6] are also utilized. Typical configurations of APPJs consist of a tube for conducting the flow of the buffer gas and set of electrodes where powered one can be in the contact with the plasma or covered by dielectric. The power supplies that are most commonly used can be divided by the type of signal as continuous or pulsed and they can operate in the large range of frequencies (from several kHz to GHz). The choice of the geometry as well as the type of the power supply used is governed by the application of the APP. Regardless of the system choice and configuration, one needs to perform detailed diagnostics of the plasma system to obtain the data that could be linked to the processes significant for the particular treatment. Then, the efficiency of desired application can be assessed and, which is also important in many cases, there is an opportunity up to some extent to compare the achieved effects with the other systems used for the same application. At the same time, the detailed diagnostics allows the studies of fundamental questions of APP behaviour. For instance, in the case of APPJs an interesting feature of formation of pulsed atmospheric-pressure streamers (PAPS) was observed and investigated. The fast ICCD imaging of this phenomena revealed that PAPS have a speed of several kilometres per second and their formation and propagation still needs to be explained in more detail. However, from the point of applications the most important feature of the APP is that they create chemically highly active media (both in gas and liquid) with the properties that can be generally tuned according to the application’s demands. The active chemistry of the plasma’s gas phase can directly modify the treated surface or activate specific mechanisms inside the treated target, e.g. plasma
treatment of cells leading to the improved differentiation or cancer cell death. Also, since APP can operate in direct contact with a liquid, the chemically active environment produced in the gas phase above the liquid can modify the physical and chemical properties of the treated liquid. Extensive research in this type of experimental configurations has led to the development of the applications of
APP in the field of agriculture. Two examples of such applications are direct treatment of seeds and
treatment of water in order to create plasma activated water. It was shown that in both cases plasma
treatment induces higher germination percentage, less contamination of the seedlings, higher water
uptake and faster plant development. These and other observed effects are the result of the interaction
of plasma formed reactive oxygen and nitrogen species (RONS) with the seed and plant cells. RONS
can trigger various biochemical mechanisms that can be observed also at molecular level through the
activity of enzymes or hormones in the seeds and plants. Here we will try to give the overview of the detailed characterization of the APP systems that were used for both medical applications and applications in agriculture. Results of time-resolved plasma imaging using fast ICCD camera will show the development of plasma structure within one period of the power signal and provide an insight in kinetic effects such as PAPS. Moreover, by using optical emission spectroscopy spectra of excited species in the gas phase will be obtained allowing the qualitative assessment of excited species above the treated liquid. The results of electrical measurements of APP sources, as another important diagnostics tool, will be presented. Comparison of the two different APP (in electrode geometry and applied voltage frequency) will be done by comparing both the results of plasma diagnostics and the response of the biological system treated by these plasma sources. Also, investigations of the applications of APP in the agriculture will be shown featuring the idea of plasma decontamination of water polluted by pesticides and its influence on germination of commercial plants.
PB  - Bratislava: Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava
C3  - Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia
T1  - Diagnostics of atmospheric pressure plasmas and their application in agriculture
SP  - 68
EP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6199
ER  - 

@conference{
author = "Puač, Nevena and Škoro, Nikola and Maletić, Dejan and Živković, Suzana and Selaković, Nenad and Malović, Gordana and Petrović, Zoran Lj",
year = "2019",
abstract = "Atmospheric pressure plasmas (APPs) have been intensively studied in the last decade due to their high
potential in the applications in medicine, biology and, lately, in agriculture. They are generated in plasma sources of various geometries and configurations and with different electrode material in order to make them efficient for large variety of applications. The types of sources used are mainly atmospheric pressure plasma jets (APPJs), but since some applications require large areas to be treated plan parallel dielectric barrier discharges [3-6] are also utilized. Typical configurations of APPJs consist of a tube for conducting the flow of the buffer gas and set of electrodes where powered one can be in the contact with the plasma or covered by dielectric. The power supplies that are most commonly used can be divided by the type of signal as continuous or pulsed and they can operate in the large range of frequencies (from several kHz to GHz). The choice of the geometry as well as the type of the power supply used is governed by the application of the APP. Regardless of the system choice and configuration, one needs to perform detailed diagnostics of the plasma system to obtain the data that could be linked to the processes significant for the particular treatment. Then, the efficiency of desired application can be assessed and, which is also important in many cases, there is an opportunity up to some extent to compare the achieved effects with the other systems used for the same application. At the same time, the detailed diagnostics allows the studies of fundamental questions of APP behaviour. For instance, in the case of APPJs an interesting feature of formation of pulsed atmospheric-pressure streamers (PAPS) was observed and investigated. The fast ICCD imaging of this phenomena revealed that PAPS have a speed of several kilometres per second and their formation and propagation still needs to be explained in more detail. However, from the point of applications the most important feature of the APP is that they create chemically highly active media (both in gas and liquid) with the properties that can be generally tuned according to the application’s demands. The active chemistry of the plasma’s gas phase can directly modify the treated surface or activate specific mechanisms inside the treated target, e.g. plasma
treatment of cells leading to the improved differentiation or cancer cell death. Also, since APP can operate in direct contact with a liquid, the chemically active environment produced in the gas phase above the liquid can modify the physical and chemical properties of the treated liquid. Extensive research in this type of experimental configurations has led to the development of the applications of
APP in the field of agriculture. Two examples of such applications are direct treatment of seeds and
treatment of water in order to create plasma activated water. It was shown that in both cases plasma
treatment induces higher germination percentage, less contamination of the seedlings, higher water
uptake and faster plant development. These and other observed effects are the result of the interaction
of plasma formed reactive oxygen and nitrogen species (RONS) with the seed and plant cells. RONS
can trigger various biochemical mechanisms that can be observed also at molecular level through the
activity of enzymes or hormones in the seeds and plants. Here we will try to give the overview of the detailed characterization of the APP systems that were used for both medical applications and applications in agriculture. Results of time-resolved plasma imaging using fast ICCD camera will show the development of plasma structure within one period of the power signal and provide an insight in kinetic effects such as PAPS. Moreover, by using optical emission spectroscopy spectra of excited species in the gas phase will be obtained allowing the qualitative assessment of excited species above the treated liquid. The results of electrical measurements of APP sources, as another important diagnostics tool, will be presented. Comparison of the two different APP (in electrode geometry and applied voltage frequency) will be done by comparing both the results of plasma diagnostics and the response of the biological system treated by these plasma sources. Also, investigations of the applications of APP in the agriculture will be shown featuring the idea of plasma decontamination of water polluted by pesticides and its influence on germination of commercial plants.",
publisher = "Bratislava: Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava",
journal = "Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia",
title = "Diagnostics of atmospheric pressure plasmas and their application in agriculture",
pages = "68-69",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6199"
}

Puač, N., Škoro, N., Maletić, D., Živković, S., Selaković, N., Malović, G.,& Petrović, Z. L.. (2019). Diagnostics of atmospheric pressure plasmas and their application in agriculture. in Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia
Bratislava: Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava., 68-69.
https://hdl.handle.net/21.15107/rcub_ibiss_6199

Puač N, Škoro N, Maletić D, Živković S, Selaković N, Malović G, Petrović ZL. Diagnostics of atmospheric pressure plasmas and their application in agriculture. in Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia. 2019;:68-69.
https://hdl.handle.net/21.15107/rcub_ibiss_6199 .

Puač, Nevena, Škoro, Nikola, Maletić, Dejan, Živković, Suzana, Selaković, Nenad, Malović, Gordana, Petrović, Zoran Lj, "Diagnostics of atmospheric pressure plasmas and their application in agriculture" in Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia (2019):68-69,
https://hdl.handle.net/21.15107/rcub_ibiss_6199 .

TY  - JOUR
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Spasić, Kosta
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4395
AB  - In this work we present results of two significantly different types of plasma treatment on Paulownia tomentosa Steud. seeds. In the first type, seeds were directly treated in low-pressure plasma and then imbibed with distilled water. In the second type, an atmospheric pressure plasma was used for obtaining plasma activated water (PAW) which is then used for imbibition of seeds. The CAT activity and protein content is evaluated during 4 d following the imbibition process, i.e., immediately after the phytochrome activation and in the 3 subsequent days. Comparison of results of treated seeds to the control group allows to correlate the enzyme activity and protein content during the initial stages of germination with plasma treatment types and treatment conditions.
PB  - Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA
T2  - Plasma Processes and Polymers
T1  - Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water
IS  - 2
VL  - 15
DO  - 10.1002/ppap.201700082
SP  - e1700082
ER  - 

@article{
author = "Puač, Nevena and Škoro, Nikola and Spasić, Kosta and Živković, Suzana and Milutinović, Milica and Malović, Gordana and Petrović, Zoran Lj",
year = "2018",
abstract = "In this work we present results of two significantly different types of plasma treatment on Paulownia tomentosa Steud. seeds. In the first type, seeds were directly treated in low-pressure plasma and then imbibed with distilled water. In the second type, an atmospheric pressure plasma was used for obtaining plasma activated water (PAW) which is then used for imbibition of seeds. The CAT activity and protein content is evaluated during 4 d following the imbibition process, i.e., immediately after the phytochrome activation and in the 3 subsequent days. Comparison of results of treated seeds to the control group allows to correlate the enzyme activity and protein content during the initial stages of germination with plasma treatment types and treatment conditions.",
publisher = "Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA",
journal = "Plasma Processes and Polymers",
title = "Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water",
number = "2",
volume = "15",
doi = "10.1002/ppap.201700082",
pages = "e1700082"
}

Puač, N., Škoro, N., Spasić, K., Živković, S., Milutinović, M., Malović, G.,& Petrović, Z. L.. (2018). Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water. in Plasma Processes and Polymers
Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA., 15(2), e1700082.
https://doi.org/10.1002/ppap.201700082

Puač N, Škoro N, Spasić K, Živković S, Milutinović M, Malović G, Petrović ZL. Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water. in Plasma Processes and Polymers. 2018;15(2):e1700082.
doi:10.1002/ppap.201700082 .

Puač, Nevena, Škoro, Nikola, Spasić, Kosta, Živković, Suzana, Milutinović, Milica, Malović, Gordana, Petrović, Zoran Lj, "Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water" in Plasma Processes and Polymers, 15, no. 2 (2018):e1700082,
https://doi.org/10.1002/ppap.201700082 . .

TY  - JOUR
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Spasić, Kosta
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj.
PY  - 2018
UR  - http://doi.wiley.com/10.1002/ppap.201700082
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3538
AB  - In this work we present results of two significantly different types of plasma treatment on Paulownia tomentosa Steud. seeds. In the first type, seeds were directly treated in low‐pressure plasma and then imbibed with distilled water. In the second type, an atmospheric pressure plasma was used for obtaining plasma activated water (PAW) which is then used for imbibition of seeds. The CAT activity and protein content is evaluated during 4 d following the imbibition process, i.e., immediately after the phytochrome activation and in the 3 subsequent days. Comparison of results of treated seeds to the control group allows to correlate the enzyme activity and protein content during the initial stages of germination with plasma treatment types and treatment
T2  - Plasma Processes and Polymers
T1  - Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water
IS  - 2
VL  - 15
DO  - 10.1002/ppap.201700082
SP  - 1700082
ER  - 

@article{
author = "Puač, Nevena and Škoro, Nikola and Spasić, Kosta and Živković, Suzana and Milutinović, Milica and Malović, Gordana and Petrović, Zoran Lj.",
year = "2018",
abstract = "In this work we present results of two significantly different types of plasma treatment on Paulownia tomentosa Steud. seeds. In the first type, seeds were directly treated in low‐pressure plasma and then imbibed with distilled water. In the second type, an atmospheric pressure plasma was used for obtaining plasma activated water (PAW) which is then used for imbibition of seeds. The CAT activity and protein content is evaluated during 4 d following the imbibition process, i.e., immediately after the phytochrome activation and in the 3 subsequent days. Comparison of results of treated seeds to the control group allows to correlate the enzyme activity and protein content during the initial stages of germination with plasma treatment types and treatment",
journal = "Plasma Processes and Polymers",
title = "Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water",
number = "2",
volume = "15",
doi = "10.1002/ppap.201700082",
pages = "1700082"
}

Puač, N., Škoro, N., Spasić, K., Živković, S., Milutinović, M., Malović, G.,& Petrović, Z. Lj.. (2018). Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water. in Plasma Processes and Polymers, 15(2), 1700082.
https://doi.org/10.1002/ppap.201700082

Puač N, Škoro N, Spasić K, Živković S, Milutinović M, Malović G, Petrović ZL. Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water. in Plasma Processes and Polymers. 2018;15(2):1700082.
doi:10.1002/ppap.201700082 .

Puač, Nevena, Škoro, Nikola, Spasić, Kosta, Živković, Suzana, Milutinović, Milica, Malović, Gordana, Petrović, Zoran Lj., "Activity of catalase enzyme in Paulownia tomentosa seeds during the process of germination after treatments with low pressure plasma and plasma activated water" in Plasma Processes and Polymers, 15, no. 2 (2018):1700082,
https://doi.org/10.1002/ppap.201700082 . .

TY  - JOUR
AU  - Škoro, Nikola
AU  - Puač, Nevena
AU  - Živković, Suzana
AU  - Krstić Milošević, Dijana
AU  - Cvelbar, Uroš
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj.
PY  - 2018
UR  - http://link.springer.com/10.1140/epjd/e2017-80329-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3030
AB  - Today’s reality is connected with mitigation of threats from the new chemical and biological warfare agents. A novel investigation of cold plasmas in contact with liquids presented in this paper demonstrated that the chemically reactive environment produced by atmospheric pressure plasma jet (APPJ) is potentially capable of rapid destruction of chemical warfare agents in a broad spectrum. The decontamination of three different chemical warfare agent surrogates dissolved in liquid is investigated by using an easily transportable APPJ. The jet is powered by a kHz signal source connected to a low-voltage DC source and with He as working gas. The detailed investigation of electrical properties is performed for various plasmas at different distances from the sample. The measurements of plasma properties in situ are supported by the optical spectrometry measurements, whereas the high performance liquid chromatography measurements before and after the treatment of aqueous solutions of Malathion, Fenitrothion and Dimethyl Methylphosphonate. These solutions are used to evaluate destruction and its efficiency for specific neural agent simulants. The particular removal rates are found to be from 56% up to 96% during 10 min treatment. The data obtained provide basis to evaluate APPJ’s efficiency at different operating conditions. The presented results are promising and could be improved with different operating conditions and optimization of the decontamination process.
T2  - The European Physical Journal D
T1  - Destruction of chemical warfare surrogates using a portable atmospheric pressure plasma jet
IS  - 1
VL  - 72
DO  - 10.1140/epjd/e2017-80329-9
SP  - 2
ER  - 

@article{
author = "Škoro, Nikola and Puač, Nevena and Živković, Suzana and Krstić Milošević, Dijana and Cvelbar, Uroš and Malović, Gordana and Petrović, Zoran Lj.",
year = "2018",
abstract = "Today’s reality is connected with mitigation of threats from the new chemical and biological warfare agents. A novel investigation of cold plasmas in contact with liquids presented in this paper demonstrated that the chemically reactive environment produced by atmospheric pressure plasma jet (APPJ) is potentially capable of rapid destruction of chemical warfare agents in a broad spectrum. The decontamination of three different chemical warfare agent surrogates dissolved in liquid is investigated by using an easily transportable APPJ. The jet is powered by a kHz signal source connected to a low-voltage DC source and with He as working gas. The detailed investigation of electrical properties is performed for various plasmas at different distances from the sample. The measurements of plasma properties in situ are supported by the optical spectrometry measurements, whereas the high performance liquid chromatography measurements before and after the treatment of aqueous solutions of Malathion, Fenitrothion and Dimethyl Methylphosphonate. These solutions are used to evaluate destruction and its efficiency for specific neural agent simulants. The particular removal rates are found to be from 56% up to 96% during 10 min treatment. The data obtained provide basis to evaluate APPJ’s efficiency at different operating conditions. The presented results are promising and could be improved with different operating conditions and optimization of the decontamination process.",
journal = "The European Physical Journal D",
title = "Destruction of chemical warfare surrogates using a portable atmospheric pressure plasma jet",
number = "1",
volume = "72",
doi = "10.1140/epjd/e2017-80329-9",
pages = "2"
}

Škoro, N., Puač, N., Živković, S., Krstić Milošević, D., Cvelbar, U., Malović, G.,& Petrović, Z. Lj.. (2018). Destruction of chemical warfare surrogates using a portable atmospheric pressure plasma jet. in The European Physical Journal D, 72(1), 2.
https://doi.org/10.1140/epjd/e2017-80329-9

Škoro N, Puač N, Živković S, Krstić Milošević D, Cvelbar U, Malović G, Petrović ZL. Destruction of chemical warfare surrogates using a portable atmospheric pressure plasma jet. in The European Physical Journal D. 2018;72(1):2.
doi:10.1140/epjd/e2017-80329-9 .

Škoro, Nikola, Puač, Nevena, Živković, Suzana, Krstić Milošević, Dijana, Cvelbar, Uroš, Malović, Gordana, Petrović, Zoran Lj., "Destruction of chemical warfare surrogates using a portable atmospheric pressure plasma jet" in The European Physical Journal D, 72, no. 1 (2018):2,
https://doi.org/10.1140/epjd/e2017-80329-9 . .

TY  - CONF
AU  - Škoro, Nikola
AU  - Puač, Nevena
AU  - Živković, Suzana
AU  - Cvelbar, Uroš
AU  - Malović, Gordana
AU  - Petrović, Zoran
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6222
AB  - Non-equilibrium atmospheric pressure plasmas (APP) are chemically active media with properties adjustable to comply with various application demands. ln biomedical field, so far APP have demonstrated excellent applicability in sterilization and cleaning, i.e. for treatment of organic materials and microorganisms. Atmospheric pressure plasma jets (APPJ) proved to be technically simple and efficient sources of cold APP which are suitable for different application setups. Recently, a new research field-plasmas in agriculture emerged, widening number of applications. Since APPJs can also operate in contact with liquids, they are suitable for new applications in plasma agriculture. Initial investigations show that chemically reactive environment produced by these sources can influence and modify physical and chemical properties of liquids. As one of the main surface water contaminants are pesticides used in agriculture our idea is to use APP for water detoxification. Namely, we conducted a study on decontamination of water samples polluted with different pesticides, i.e. organophosphate compounds, by using APPJs. We used two different jet configurations powered by a kHz signal source with He as working gas and treated liquid samples at different distances between the jet and the sample surface, for different duration times and for different liquid sample volumes. Optical and electrical characterization of the APPJs is performed in order to obtain information about the plasma properties and stability of the treatment conditions. Liquid sample analysis before and after the treatment are executed by using high performance liquid chromatography (HPLC) and liquid chromatography coupled with mass spectrometry (LC-MS) in order to follow degradation of organophosphates. Significant and efficient degradation of both pesticides is noticed and appearance of degradation products is observed in the liquid sample. Dependence of the decontamination efficiency on treatment time is determined. We also investigated toxicity of degradation products. Thus, from the data obtained we could assess the decontamination efficiency for different APPJ types and also to provide information about mechanisms governing the plasma destruction of organophosphates.
PB  - Zagreb: Institute of Physics
C3  - Scientific Program and Book of abstracts: 7th Central European Symposium on Plasma Chemistry; 2017 Sep 3-7; Sveti Martin na Muri, Croatia
T1  - Use of atmospheric pressure plasmas for decontamination of water containing organophosphates
SP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6222
ER  - 

@conference{
author = "Škoro, Nikola and Puač, Nevena and Živković, Suzana and Cvelbar, Uroš and Malović, Gordana and Petrović, Zoran",
year = "2017",
abstract = "Non-equilibrium atmospheric pressure plasmas (APP) are chemically active media with properties adjustable to comply with various application demands. ln biomedical field, so far APP have demonstrated excellent applicability in sterilization and cleaning, i.e. for treatment of organic materials and microorganisms. Atmospheric pressure plasma jets (APPJ) proved to be technically simple and efficient sources of cold APP which are suitable for different application setups. Recently, a new research field-plasmas in agriculture emerged, widening number of applications. Since APPJs can also operate in contact with liquids, they are suitable for new applications in plasma agriculture. Initial investigations show that chemically reactive environment produced by these sources can influence and modify physical and chemical properties of liquids. As one of the main surface water contaminants are pesticides used in agriculture our idea is to use APP for water detoxification. Namely, we conducted a study on decontamination of water samples polluted with different pesticides, i.e. organophosphate compounds, by using APPJs. We used two different jet configurations powered by a kHz signal source with He as working gas and treated liquid samples at different distances between the jet and the sample surface, for different duration times and for different liquid sample volumes. Optical and electrical characterization of the APPJs is performed in order to obtain information about the plasma properties and stability of the treatment conditions. Liquid sample analysis before and after the treatment are executed by using high performance liquid chromatography (HPLC) and liquid chromatography coupled with mass spectrometry (LC-MS) in order to follow degradation of organophosphates. Significant and efficient degradation of both pesticides is noticed and appearance of degradation products is observed in the liquid sample. Dependence of the decontamination efficiency on treatment time is determined. We also investigated toxicity of degradation products. Thus, from the data obtained we could assess the decontamination efficiency for different APPJ types and also to provide information about mechanisms governing the plasma destruction of organophosphates.",
publisher = "Zagreb: Institute of Physics",
journal = "Scientific Program and Book of abstracts: 7th Central European Symposium on Plasma Chemistry; 2017 Sep 3-7; Sveti Martin na Muri, Croatia",
title = "Use of atmospheric pressure plasmas for decontamination of water containing organophosphates",
pages = "28",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6222"
}

Škoro, N., Puač, N., Živković, S., Cvelbar, U., Malović, G.,& Petrović, Z.. (2017). Use of atmospheric pressure plasmas for decontamination of water containing organophosphates. in Scientific Program and Book of abstracts: 7th Central European Symposium on Plasma Chemistry; 2017 Sep 3-7; Sveti Martin na Muri, Croatia
Zagreb: Institute of Physics., 28.
https://hdl.handle.net/21.15107/rcub_ibiss_6222

Škoro N, Puač N, Živković S, Cvelbar U, Malović G, Petrović Z. Use of atmospheric pressure plasmas for decontamination of water containing organophosphates. in Scientific Program and Book of abstracts: 7th Central European Symposium on Plasma Chemistry; 2017 Sep 3-7; Sveti Martin na Muri, Croatia. 2017;:28.
https://hdl.handle.net/21.15107/rcub_ibiss_6222 .

Škoro, Nikola, Puač, Nevena, Živković, Suzana, Cvelbar, Uroš, Malović, Gordana, Petrović, Zoran, "Use of atmospheric pressure plasmas for decontamination of water containing organophosphates" in Scientific Program and Book of abstracts: 7th Central European Symposium on Plasma Chemistry; 2017 Sep 3-7; Sveti Martin na Muri, Croatia (2017):28,
https://hdl.handle.net/21.15107/rcub_ibiss_6222 .

TY  - CONF
AU  - Škoro, Nikola
AU  - Puač, Nevena
AU  - Živković, Suzana
AU  - Mišić, Danijela
AU  - Cvelbar, Uroš
AU  - Malović, Gordana
AU  - Petrović, Zoran
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6221
AB  - Nonequilibrium atmospheric pressure plasma proved to produce reactive chemistry environment at gas temperatures as low as room temperature which is perfect for treatments of different kind of surfaces. Over the past several years cold atmospheric pressure plasma applications in surface sterilization and cleaning have been extensively studied. Recently, the research has been directed to utilization of this type of plasma far agriculture. Plasma is used directly for treatment of seeds, but, also, indirectly through plasma activated water. In the case of decontamination of water contaminated with conventional pesticides and insecticides plasma can play important role. One of suitable plasma sources for these treatments are atmospheric pressure plasma jets (APPJ), since they are small-size (easily scalable to larger systems), relatively technically simple and cost-effective devices. In our case, we investigated treatments of water contaminated with three types of pesticides. Decontamination is achieved by applying the jet on the liquid surface generating high-energy electrons, reactive oxygen and nitrogen species, radicals and ultraviolet radiation. Reactive species created al the gas-liquid interface effectively degrade organic pollutants in the water. However, due to the complexity of the system, key mechanisms important for destruction of particular contaminant are not clearly known. Moreover, optimisation of the system is recondite by reciprocal influence of the jet and surface. Thus, we have performed detailed and systematic investigation of the APPJ-liquid sample system properties at different conditions and far different pollutants dissolved in water. Electrical and optical measurements provided information about the discharge running at different powers and distances from the sample. We could relate electrical characteristics with spatial profiles of the discharge. Moreover, optical spectrometry provided data on emission lines characteristic far the plasma used far decontamination. By using liquid chromatography measurements of contaminated water samples before and after the treatment we acquired evidence on destruction of specific pollutants and found decontamination rates. AII this data is valuable far evaluation of APPJ efficiency at different operating conditions and optimisation of the decontamination process.
PB  - lnstitute of Plasma Physics CAS
C3  - Conference Program and Book of Abstract of the International Conference on Plasmas with Liquids (ICPL 2017); 2017 Mar 5-9; Prague, Czech Republic
T1  - Application of atmospheric pressure plasmas in agriculture for wastewater cleaning
SP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6221
ER  - 

@conference{
author = "Škoro, Nikola and Puač, Nevena and Živković, Suzana and Mišić, Danijela and Cvelbar, Uroš and Malović, Gordana and Petrović, Zoran",
year = "2017",
abstract = "Nonequilibrium atmospheric pressure plasma proved to produce reactive chemistry environment at gas temperatures as low as room temperature which is perfect for treatments of different kind of surfaces. Over the past several years cold atmospheric pressure plasma applications in surface sterilization and cleaning have been extensively studied. Recently, the research has been directed to utilization of this type of plasma far agriculture. Plasma is used directly for treatment of seeds, but, also, indirectly through plasma activated water. In the case of decontamination of water contaminated with conventional pesticides and insecticides plasma can play important role. One of suitable plasma sources for these treatments are atmospheric pressure plasma jets (APPJ), since they are small-size (easily scalable to larger systems), relatively technically simple and cost-effective devices. In our case, we investigated treatments of water contaminated with three types of pesticides. Decontamination is achieved by applying the jet on the liquid surface generating high-energy electrons, reactive oxygen and nitrogen species, radicals and ultraviolet radiation. Reactive species created al the gas-liquid interface effectively degrade organic pollutants in the water. However, due to the complexity of the system, key mechanisms important for destruction of particular contaminant are not clearly known. Moreover, optimisation of the system is recondite by reciprocal influence of the jet and surface. Thus, we have performed detailed and systematic investigation of the APPJ-liquid sample system properties at different conditions and far different pollutants dissolved in water. Electrical and optical measurements provided information about the discharge running at different powers and distances from the sample. We could relate electrical characteristics with spatial profiles of the discharge. Moreover, optical spectrometry provided data on emission lines characteristic far the plasma used far decontamination. By using liquid chromatography measurements of contaminated water samples before and after the treatment we acquired evidence on destruction of specific pollutants and found decontamination rates. AII this data is valuable far evaluation of APPJ efficiency at different operating conditions and optimisation of the decontamination process.",
publisher = "lnstitute of Plasma Physics CAS",
journal = "Conference Program and Book of Abstract of the International Conference on Plasmas with Liquids (ICPL 2017); 2017 Mar 5-9; Prague, Czech Republic",
title = "Application of atmospheric pressure plasmas in agriculture for wastewater cleaning",
pages = "13",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6221"
}

Škoro, N., Puač, N., Živković, S., Mišić, D., Cvelbar, U., Malović, G.,& Petrović, Z.. (2017). Application of atmospheric pressure plasmas in agriculture for wastewater cleaning. in Conference Program and Book of Abstract of the International Conference on Plasmas with Liquids (ICPL 2017); 2017 Mar 5-9; Prague, Czech Republic
lnstitute of Plasma Physics CAS., 13.
https://hdl.handle.net/21.15107/rcub_ibiss_6221

Škoro N, Puač N, Živković S, Mišić D, Cvelbar U, Malović G, Petrović Z. Application of atmospheric pressure plasmas in agriculture for wastewater cleaning. in Conference Program and Book of Abstract of the International Conference on Plasmas with Liquids (ICPL 2017); 2017 Mar 5-9; Prague, Czech Republic. 2017;:13.
https://hdl.handle.net/21.15107/rcub_ibiss_6221 .

Škoro, Nikola, Puač, Nevena, Živković, Suzana, Mišić, Danijela, Cvelbar, Uroš, Malović, Gordana, Petrović, Zoran, "Application of atmospheric pressure plasmas in agriculture for wastewater cleaning" in Conference Program and Book of Abstract of the International Conference on Plasmas with Liquids (ICPL 2017); 2017 Mar 5-9; Prague, Czech Republic (2017):13,
https://hdl.handle.net/21.15107/rcub_ibiss_6221 .

TY  - CONF
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Spasić, Kosta
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Šašić, Vuk
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6198
AB  - In this abstract we report on influence of direct and indirect plasma treatments on catalase enzyme activity in Paulownia tomentosa seeds. The direct treatment of the seeds was performed in low-pressure RF plasma system for different treatment times. After treatments these seeds were imbibed with distilled water. The other set of P. tomentosa seeds was imbibed with plasma activated water (PAW). PAW was produced by using atmospheric pressure plasma source in treatments with different durations. Seeds from both sets were exposed to the same conditions and after 5 days activity of catalase enzyme was measured. In comparison to the control sample, differences in the activity was observed both regarding direct and PAW treated seeds and regarding duration of treatments.
PB  - Lisboa: Instituto de Plasmas e Fusão Nuclear, Instituto Superior Técnico, Universidade de Lisboa
C3  - Proceedings of the 23rd International Conference on Phenomena in Ionized Gases (ICPIG); 2017 Jul 9-14; Estoril, Portugal
T1  - Activity of catalase enzyme in P. tomentosa seeds after direct plasma treatments and treatments with plasma activated water
SP  - 180
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6198
ER  - 

@conference{
author = "Puač, Nevena and Škoro, Nikola and Spasić, Kosta and Živković, Suzana and Milutinović, Milica and Šašić, Vuk and Malović, Gordana and Petrović, Zoran Lj",
year = "2017",
abstract = "In this abstract we report on influence of direct and indirect plasma treatments on catalase enzyme activity in Paulownia tomentosa seeds. The direct treatment of the seeds was performed in low-pressure RF plasma system for different treatment times. After treatments these seeds were imbibed with distilled water. The other set of P. tomentosa seeds was imbibed with plasma activated water (PAW). PAW was produced by using atmospheric pressure plasma source in treatments with different durations. Seeds from both sets were exposed to the same conditions and after 5 days activity of catalase enzyme was measured. In comparison to the control sample, differences in the activity was observed both regarding direct and PAW treated seeds and regarding duration of treatments.",
publisher = "Lisboa: Instituto de Plasmas e Fusão Nuclear, Instituto Superior Técnico, Universidade de Lisboa",
journal = "Proceedings of the 23rd International Conference on Phenomena in Ionized Gases (ICPIG); 2017 Jul 9-14; Estoril, Portugal",
title = "Activity of catalase enzyme in P. tomentosa seeds after direct plasma treatments and treatments with plasma activated water",
pages = "180",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6198"
}

Puač, N., Škoro, N., Spasić, K., Živković, S., Milutinović, M., Šašić, V., Malović, G.,& Petrović, Z. L.. (2017). Activity of catalase enzyme in P. tomentosa seeds after direct plasma treatments and treatments with plasma activated water. in Proceedings of the 23rd International Conference on Phenomena in Ionized Gases (ICPIG); 2017 Jul 9-14; Estoril, Portugal
Lisboa: Instituto de Plasmas e Fusão Nuclear, Instituto Superior Técnico, Universidade de Lisboa., 180.
https://hdl.handle.net/21.15107/rcub_ibiss_6198

Puač N, Škoro N, Spasić K, Živković S, Milutinović M, Šašić V, Malović G, Petrović ZL. Activity of catalase enzyme in P. tomentosa seeds after direct plasma treatments and treatments with plasma activated water. in Proceedings of the 23rd International Conference on Phenomena in Ionized Gases (ICPIG); 2017 Jul 9-14; Estoril, Portugal. 2017;:180.
https://hdl.handle.net/21.15107/rcub_ibiss_6198 .

Puač, Nevena, Škoro, Nikola, Spasić, Kosta, Živković, Suzana, Milutinović, Milica, Šašić, Vuk, Malović, Gordana, Petrović, Zoran Lj, "Activity of catalase enzyme in P. tomentosa seeds after direct plasma treatments and treatments with plasma activated water" in Proceedings of the 23rd International Conference on Phenomena in Ionized Gases (ICPIG); 2017 Jul 9-14; Estoril, Portugal (2017):180,
https://hdl.handle.net/21.15107/rcub_ibiss_6198 .

TY  - CONF
AU  - Živković, Suzana
AU  - Skorić, Marijana
AU  - Ristić, Mihailo
AU  - Perišić, Mirjana
AU  - Puač, Nevena
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6190
AB  - Volatile organic compounds (VOC) emitted by fronds of desiccation tolerant fern Asplenium ceterach L. during the rehydration process were investigated. Head-space GC-MS analysis was used in order to determine the volatile compounds in dry and rehydrated sample of golden fern. It was found that the VOC profile of golden fern is based mainly on isomeric heptadienals (over 25%) and decadienals (over 20%), other linear aldehydes, alcohols and related compounds. Aerial parts of fresh and dry fronds do not contain monoterpene-, sesquiterpene- and diterpene-type hydrocarbons or corresponding terpenoids. In order to determine the composition of the VOC during the process of rehydration, we have applied proton-transfer reaction mass spectrometry (PTR-MS). PTR-MS is a sensitive technique that allows real-time detection of VOCs emitted from plants. We have used dry plants to establish a base line and then distilled water was added to induce hydration process of the golden fern plant. Masses in the range from m/z 21 to m/z 300 were measured with dwell time of 200 ms and for the time period of 24 hours. The experiment was repeated for five plants. PTR-MS measurements revealed that the amounts of compounds, determined by head-space GC-MS, generally exhibit a significant increase after addition of water. After about 20 hours the values measured by PTR-MS for these masses are reduced to a new base line that corresponds to the rehydrated plant.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Volatile compounds of golden fern (Asplenium ceterach L.) detected during the process of rehydration
SP  - 187
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6190
ER  - 

@conference{
author = "Živković, Suzana and Skorić, Marijana and Ristić, Mihailo and Perišić, Mirjana and Puač, Nevena and Malović, Gordana and Petrović, Zoran Lj",
year = "2015",
abstract = "Volatile organic compounds (VOC) emitted by fronds of desiccation tolerant fern Asplenium ceterach L. during the rehydration process were investigated. Head-space GC-MS analysis was used in order to determine the volatile compounds in dry and rehydrated sample of golden fern. It was found that the VOC profile of golden fern is based mainly on isomeric heptadienals (over 25%) and decadienals (over 20%), other linear aldehydes, alcohols and related compounds. Aerial parts of fresh and dry fronds do not contain monoterpene-, sesquiterpene- and diterpene-type hydrocarbons or corresponding terpenoids. In order to determine the composition of the VOC during the process of rehydration, we have applied proton-transfer reaction mass spectrometry (PTR-MS). PTR-MS is a sensitive technique that allows real-time detection of VOCs emitted from plants. We have used dry plants to establish a base line and then distilled water was added to induce hydration process of the golden fern plant. Masses in the range from m/z 21 to m/z 300 were measured with dwell time of 200 ms and for the time period of 24 hours. The experiment was repeated for five plants. PTR-MS measurements revealed that the amounts of compounds, determined by head-space GC-MS, generally exhibit a significant increase after addition of water. After about 20 hours the values measured by PTR-MS for these masses are reduced to a new base line that corresponds to the rehydrated plant.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Volatile compounds of golden fern (Asplenium ceterach L.) detected during the process of rehydration",
pages = "187",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6190"
}

Živković, S., Skorić, M., Ristić, M., Perišić, M., Puač, N., Malović, G.,& Petrović, Z. L.. (2015). Volatile compounds of golden fern (Asplenium ceterach L.) detected during the process of rehydration. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society., 187.
https://hdl.handle.net/21.15107/rcub_ibiss_6190

Živković S, Skorić M, Ristić M, Perišić M, Puač N, Malović G, Petrović ZL. Volatile compounds of golden fern (Asplenium ceterach L.) detected during the process of rehydration. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:187.
https://hdl.handle.net/21.15107/rcub_ibiss_6190 .

Živković, Suzana, Skorić, Marijana, Ristić, Mihailo, Perišić, Mirjana, Puač, Nevena, Malović, Gordana, Petrović, Zoran Lj, "Volatile compounds of golden fern (Asplenium ceterach L.) detected during the process of rehydration" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):187,
https://hdl.handle.net/21.15107/rcub_ibiss_6190 .

TY  - CONF
AU  - Selaković, Nenad
AU  - Jevremović, Slađana
AU  - Živković, Suzana
AU  - Maletić, Dejan
AU  - Puač, Nevena
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6396
AB  - The effects of atmospheric pressure plasmas on living cells and tissues have been studied on numerous occasions in recent literature. lt appears that plasma treatment may find many biomedical applications. There are many types of plasmas that can be generated under ambient pressure and temperature conditions. Plas­ma needle is one of the atmospheric pressure devices that meet the requirements of precise and localized treatment necessary for treatment of plant cells. We have investigated the effect of atmospheric pressure plasma generated by plasma needle device, under ambient pressure and temperature conditions, on carrot somatic embryogenesis. The embryogenic calli cul­tures of carrot (Daucus carrota) were established from storage root explants and maintained on Murashige and Skoog medium (MS) supplemented with 2,4-dichlorphenoxyacetic acid (2,4-D, 1.0 mg L·1) and cultured in dark. Calli samples (10-30 mg) were placed in 96 wells micro-titer plate before the tip of the plasma needle was placed at the edge of the well when plasma was covering whole surface of the samples. The treatment times were 1 O, 30, 60 and 120 s at two powers, namely 0.4 W and 1.4 W. After plasma treatment, calli samples were cultured on basal MS medium without plant growth regulators or on MS medium supplemented with 2,4-D or 2,4-D and kinetin (1.0 mg L-1, each) and cultured in dark for six weeks. Calli number, fresh weight in­crease and developmental stage of formed somatic embryos were estimated. lt was shown that plasma treat­ment notably stimulated growth and somatic embryo formation of calli cultured on basal MS medium. The highest fresh weight increase (-40 fold) was observed after 30 s of plasma needle treatment at 0.4 W. The number and developmental stage of formed somatic embryos depended on duration and power of plasma treatment, as well as the type of culture media. The highest number of somatic embryos was observed when calli samples were grown on basal MS medium after 60 s of plasma treatment at 1.4 W. Furthermore, plasma treatment significantly increased the number of formed somatic embryos on MS media supplemented with plant growth regulators. Our results show that plasma strongly affects growth and somatic embryo forma­tion and development of carrot calli.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
T1  - The effects of atmospheric pressure plasma on somatic embryogenesis  of carrot (Daucus carrota)
SP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6396
ER  - 

@conference{
author = "Selaković, Nenad and Jevremović, Slađana and Živković, Suzana and Maletić, Dejan and Puač, Nevena and Malović, Gordana and Petrović, Zoran Lj",
year = "2013",
abstract = "The effects of atmospheric pressure plasmas on living cells and tissues have been studied on numerous occasions in recent literature. lt appears that plasma treatment may find many biomedical applications. There are many types of plasmas that can be generated under ambient pressure and temperature conditions. Plas­ma needle is one of the atmospheric pressure devices that meet the requirements of precise and localized treatment necessary for treatment of plant cells. We have investigated the effect of atmospheric pressure plasma generated by plasma needle device, under ambient pressure and temperature conditions, on carrot somatic embryogenesis. The embryogenic calli cul­tures of carrot (Daucus carrota) were established from storage root explants and maintained on Murashige and Skoog medium (MS) supplemented with 2,4-dichlorphenoxyacetic acid (2,4-D, 1.0 mg L·1) and cultured in dark. Calli samples (10-30 mg) were placed in 96 wells micro-titer plate before the tip of the plasma needle was placed at the edge of the well when plasma was covering whole surface of the samples. The treatment times were 1 O, 30, 60 and 120 s at two powers, namely 0.4 W and 1.4 W. After plasma treatment, calli samples were cultured on basal MS medium without plant growth regulators or on MS medium supplemented with 2,4-D or 2,4-D and kinetin (1.0 mg L-1, each) and cultured in dark for six weeks. Calli number, fresh weight in­crease and developmental stage of formed somatic embryos were estimated. lt was shown that plasma treat­ment notably stimulated growth and somatic embryo formation of calli cultured on basal MS medium. The highest fresh weight increase (-40 fold) was observed after 30 s of plasma needle treatment at 0.4 W. The number and developmental stage of formed somatic embryos depended on duration and power of plasma treatment, as well as the type of culture media. The highest number of somatic embryos was observed when calli samples were grown on basal MS medium after 60 s of plasma treatment at 1.4 W. Furthermore, plasma treatment significantly increased the number of formed somatic embryos on MS media supplemented with plant growth regulators. Our results show that plasma strongly affects growth and somatic embryo forma­tion and development of carrot calli.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia",
title = "The effects of atmospheric pressure plasma on somatic embryogenesis  of carrot (Daucus carrota)",
pages = "36",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6396"
}

Selaković, N., Jevremović, S., Živković, S., Maletić, D., Puač, N., Malović, G.,& Petrović, Z. L.. (2013). The effects of atmospheric pressure plasma on somatic embryogenesis  of carrot (Daucus carrota). in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
Belgrade: Serbian Plant Physiology Society., 36.
https://hdl.handle.net/21.15107/rcub_ibiss_6396

Selaković N, Jevremović S, Živković S, Maletić D, Puač N, Malović G, Petrović ZL. The effects of atmospheric pressure plasma on somatic embryogenesis  of carrot (Daucus carrota). in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia. 2013;:36.
https://hdl.handle.net/21.15107/rcub_ibiss_6396 .

Selaković, Nenad, Jevremović, Slađana, Živković, Suzana, Maletić, Dejan, Puač, Nevena, Malović, Gordana, Petrović, Zoran Lj, "The effects of atmospheric pressure plasma on somatic embryogenesis  of carrot (Daucus carrota)" in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia (2013):36,
https://hdl.handle.net/21.15107/rcub_ibiss_6396 .

TY  - JOUR
AU  - Puac, Nevena
AU  - Petrović, Zoran Lj
AU  - Malović, Gordana N
AU  - Đorđević, Antonije R
AU  - Živković, Suzana
AU  - Giba, Zlatko
AU  - Grubišić, Dragoljub
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1638
AB  - In this paper we present voltage-current-power characteristics of a plasma needle operating in the flow of helium at atmospheric pressure. In addition, we show some examples of how such a plasma affects plant tissues. In the characterization of the plasma needle, current and voltage waveforms were recorded by two derivative probes. These two probes are similar to the probes previously used by Puac et al for measuring transmitted power in low pressure CCP rf discharge. The instantaneous power was calculated from current and voltage waveforms and U-I characteristics of the discharge were determined. Regimes of operation with and without the grounding ring at the tip of the needle were considered. We have chosen two model systems to study the effect of the plasma needle on plant cells and tissues: sweet fern gametophyte (prothallus) and calli produced in vitro. Since the prothallus consists of a single layer of cells, the cytological effects could be easily examined. In addition, calli and prothallus are easy to manipulate and in vitro culture provides a possibility to work under constant and controlled conditions.
T2  - Journal of Physics D-Applied Physics
T1  - Measurements of voltage-current characteristics of a plasma needle and its effect on plant cells
IS  - 16
VL  - 39
EP  - 3519
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1638
ER  - 

@article{
author = "Puac, Nevena and Petrović, Zoran Lj and Malović, Gordana N and Đorđević, Antonije R and Živković, Suzana and Giba, Zlatko and Grubišić, Dragoljub",
year = "2006",
abstract = "In this paper we present voltage-current-power characteristics of a plasma needle operating in the flow of helium at atmospheric pressure. In addition, we show some examples of how such a plasma affects plant tissues. In the characterization of the plasma needle, current and voltage waveforms were recorded by two derivative probes. These two probes are similar to the probes previously used by Puac et al for measuring transmitted power in low pressure CCP rf discharge. The instantaneous power was calculated from current and voltage waveforms and U-I characteristics of the discharge were determined. Regimes of operation with and without the grounding ring at the tip of the needle were considered. We have chosen two model systems to study the effect of the plasma needle on plant cells and tissues: sweet fern gametophyte (prothallus) and calli produced in vitro. Since the prothallus consists of a single layer of cells, the cytological effects could be easily examined. In addition, calli and prothallus are easy to manipulate and in vitro culture provides a possibility to work under constant and controlled conditions.",
journal = "Journal of Physics D-Applied Physics",
title = "Measurements of voltage-current characteristics of a plasma needle and its effect on plant cells",
number = "16",
volume = "39",
pages = "3519",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1638"
}

Puac, N., Petrović, Z. L., Malović, G. N., Đorđević, A. R., Živković, S., Giba, Z.,& Grubišić, D.. (2006). Measurements of voltage-current characteristics of a plasma needle and its effect on plant cells. in Journal of Physics D-Applied Physics, 39(16).
https://hdl.handle.net/21.15107/rcub_ibiss_1638

Puac N, Petrović ZL, Malović GN, Đorđević AR, Živković S, Giba Z, Grubišić D. Measurements of voltage-current characteristics of a plasma needle and its effect on plant cells. in Journal of Physics D-Applied Physics. 2006;39(16):null-3519.
https://hdl.handle.net/21.15107/rcub_ibiss_1638 .

Puac, Nevena, Petrović, Zoran Lj, Malović, Gordana N, Đorđević, Antonije R, Živković, Suzana, Giba, Zlatko, Grubišić, Dragoljub, "Measurements of voltage-current characteristics of a plasma needle and its effect on plant cells" in Journal of Physics D-Applied Physics, 39, no. 16 (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1638 .