TY  - JOUR
AU  - Grković, Ivana
AU  - Bjelobaba, Ivana
AU  - Mitrović, Nataša
AU  - Lavrnja, Irena
AU  - Drakulić, Dunja
AU  - Martinović, Jelena
AU  - Stanojlović, Miloš
AU  - Horvat, Anica
AU  - Nedeljković, Nadežda
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5977
AB  - Nucleoside triphosphate diphosphohydrolase3 (NTPDase3) is membrane-bound ectoenzyme which hydrolyzes extracellular ATP, thus modulating the function of purinergicreceptors and the pattern of purinergic signaling. Here we analyzed the developmentalexpression of NTPDase3 in female hypothalamus, cerebral cortex and hippocampal formationat different postnatal ages (PD7–PD90) by qRT-PCR and immunohistochemistry. Inhypothalamus and hippocampus, a similar developmental profile was seen: NTPDase3 geneexpression was stable during postnatal development and increased in adults. In the cortex,upregulation of NTPDase3 mRNA expression was seen at PD15 and further increase wasevidenced in adults. Immunohistochemical analysis at PD7 revealed faint neuronal NTPDase3localization, in dorsal hypothalamus. The immunoreactivity (ir) gradually increased in PD15and PD20, in clusters of cells in the lateral, ventral and dorsomedial hypothalamus.Furthermore, in PD20 animals, NTPDase3-ir was detected on short fibers in the posteriorhypothalamic area, while in PD30 the fibers appeared progressively longer and markedlyvaricose. In adults, the strongest NTPDase3-ir was observed in collections of cells indorsomedial hypothalamic nucleus, dorsal and lateral hypothalamus and in several thalamicareas, whereas the varicose fibers traversed entire diencephalon, particularly paraventricularthalamic nucleus, ventromedial and dorsomedial hypothalamic nuclei, the arcuate nucleus andthe prefornical part of the lateral hypothalamus. The presumably ascending NTPDase3-irfibers were first observed in PD20; their density and the varicose appearance increased untilthe adulthood. Prominent enhancement of NTPDase3-ir in the hypothalamus coincides withage when animals acquire diurnal rhythms of sleeping and feeding, supporting the hypothesisthat this enzyme may be involved in regulation of homeostatic functions.
PB  - Amsterdam: Elsevier Science Publisher
T2  - Journal of chemical neuroanatomy
T1  - Expression of ecto-nucleoside triphosphate diphosphohydrolase3 (NTPDase3) in the female rat brain during postnatal development
VL  - 77
DO  - 10.1016/j.jchemneu.2016.04.001
SP  - 10
EP  - 18
ER  - 

@article{
author = "Grković, Ivana and Bjelobaba, Ivana and Mitrović, Nataša and Lavrnja, Irena and Drakulić, Dunja and Martinović, Jelena and Stanojlović, Miloš and Horvat, Anica and Nedeljković, Nadežda",
year = "2016",
abstract = "Nucleoside triphosphate diphosphohydrolase3 (NTPDase3) is membrane-bound ectoenzyme which hydrolyzes extracellular ATP, thus modulating the function of purinergicreceptors and the pattern of purinergic signaling. Here we analyzed the developmentalexpression of NTPDase3 in female hypothalamus, cerebral cortex and hippocampal formationat different postnatal ages (PD7–PD90) by qRT-PCR and immunohistochemistry. Inhypothalamus and hippocampus, a similar developmental profile was seen: NTPDase3 geneexpression was stable during postnatal development and increased in adults. In the cortex,upregulation of NTPDase3 mRNA expression was seen at PD15 and further increase wasevidenced in adults. Immunohistochemical analysis at PD7 revealed faint neuronal NTPDase3localization, in dorsal hypothalamus. The immunoreactivity (ir) gradually increased in PD15and PD20, in clusters of cells in the lateral, ventral and dorsomedial hypothalamus.Furthermore, in PD20 animals, NTPDase3-ir was detected on short fibers in the posteriorhypothalamic area, while in PD30 the fibers appeared progressively longer and markedlyvaricose. In adults, the strongest NTPDase3-ir was observed in collections of cells indorsomedial hypothalamic nucleus, dorsal and lateral hypothalamus and in several thalamicareas, whereas the varicose fibers traversed entire diencephalon, particularly paraventricularthalamic nucleus, ventromedial and dorsomedial hypothalamic nuclei, the arcuate nucleus andthe prefornical part of the lateral hypothalamus. The presumably ascending NTPDase3-irfibers were first observed in PD20; their density and the varicose appearance increased untilthe adulthood. Prominent enhancement of NTPDase3-ir in the hypothalamus coincides withage when animals acquire diurnal rhythms of sleeping and feeding, supporting the hypothesisthat this enzyme may be involved in regulation of homeostatic functions.",
publisher = "Amsterdam: Elsevier Science Publisher",
journal = "Journal of chemical neuroanatomy",
title = "Expression of ecto-nucleoside triphosphate diphosphohydrolase3 (NTPDase3) in the female rat brain during postnatal development",
volume = "77",
doi = "10.1016/j.jchemneu.2016.04.001",
pages = "10-18"
}

Grković, I., Bjelobaba, I., Mitrović, N., Lavrnja, I., Drakulić, D., Martinović, J., Stanojlović, M., Horvat, A.,& Nedeljković, N.. (2016). Expression of ecto-nucleoside triphosphate diphosphohydrolase3 (NTPDase3) in the female rat brain during postnatal development. in Journal of chemical neuroanatomy
Amsterdam: Elsevier Science Publisher., 77, 10-18.
https://doi.org/10.1016/j.jchemneu.2016.04.001

Grković I, Bjelobaba I, Mitrović N, Lavrnja I, Drakulić D, Martinović J, Stanojlović M, Horvat A, Nedeljković N. Expression of ecto-nucleoside triphosphate diphosphohydrolase3 (NTPDase3) in the female rat brain during postnatal development. in Journal of chemical neuroanatomy. 2016;77:10-18.
doi:10.1016/j.jchemneu.2016.04.001 .

Grković, Ivana, Bjelobaba, Ivana, Mitrović, Nataša, Lavrnja, Irena, Drakulić, Dunja, Martinović, Jelena, Stanojlović, Miloš, Horvat, Anica, Nedeljković, Nadežda, "Expression of ecto-nucleoside triphosphate diphosphohydrolase3 (NTPDase3) in the female rat brain during postnatal development" in Journal of chemical neuroanatomy, 77 (2016):10-18,
https://doi.org/10.1016/j.jchemneu.2016.04.001 . .

TY  - JOUR
AU  - Grković, Ivana
AU  - Bjelobaba, Ivana
AU  - Mitrović, Nataša
AU  - Lavrnja, Irena
AU  - Drakulić, Dunja
AU  - Martinović, Jelena
AU  - Stanojlović, Miloš
AU  - Horvat, Anica
AU  - Nedeljković, Nadežda
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5976
AB  - Nucleoside triphosphate diphosphohydrolase3 (NTPDase3) is membrane-bound ectoenzyme which hydrolyzes extracellular ATP, thus modulating the function of purinergic
receptors and the pattern of purinergic signaling. Here we analyzed the developmental
expression of NTPDase3 in female hypothalamus, cerebral cortex and hippocampal formation
at different postnatal ages (PD7–PD90) by qRT-PCR and immunohistochemistry. In
hypothalamus and hippocampus, a similar developmental profile was seen: NTPDase3 gene
expression was stable during postnatal development and increased in adults. In the cortex,
upregulation of NTPDase3 mRNA expression was seen at PD15 and further increase was
evidenced in adults. Immunohistochemical analysis at PD7 revealed faint neuronal NTPDase3
localization, in dorsal hypothalamus. The immunoreactivity (ir) gradually increased in PD15
and PD20, in clusters of cells in the lateral, ventral and dorsomedial hypothalamus.
Furthermore, in PD20 animals, NTPDase3-ir was detected on short fibers in the posterior
hypothalamic area, while in PD30 the fibers appeared progressively longer and markedly
varicose. In adults, the strongest NTPDase3-ir was observed in collections of cells in
dorsomedial hypothalamic nucleus, dorsal and lateral hypothalamus and in several thalamic
areas, whereas the varicose fibers traversed entire diencephalon, particularly paraventricular
thalamic nucleus, ventromedial and dorsomedial hypothalamic nuclei, the arcuate nucleus and
the prefornical part of the lateral hypothalamus. The presumably ascending NTPDase3-ir
fibers were first observed in PD20; their density and the varicose appearance increased until
the adulthood. Prominent enhancement of NTPDase3-ir in the hypothalamus coincides with
age when animals acquire diurnal rhythms of sleeping and feeding, supporting the hypothesis
that this enzyme may be involved in regulation of homeostatic functions.
PB  - Amsterdam: Elsevier Science Publisher
T2  - Journal of chemical neuroanatomy
T1  - Expression of ecto-nucleoside triphosphate diphosphohydrolase3 (NTPDase3) in the female rat brain during postnatal development
VL  - 77
DO  - 10.1016/j.jchemneu.2016.04.001
SP  - 10
EP  - 18
ER  - 

@article{
author = "Grković, Ivana and Bjelobaba, Ivana and Mitrović, Nataša and Lavrnja, Irena and Drakulić, Dunja and Martinović, Jelena and Stanojlović, Miloš and Horvat, Anica and Nedeljković, Nadežda",
year = "2016",
abstract = "Nucleoside triphosphate diphosphohydrolase3 (NTPDase3) is membrane-bound ectoenzyme which hydrolyzes extracellular ATP, thus modulating the function of purinergic
receptors and the pattern of purinergic signaling. Here we analyzed the developmental
expression of NTPDase3 in female hypothalamus, cerebral cortex and hippocampal formation
at different postnatal ages (PD7–PD90) by qRT-PCR and immunohistochemistry. In
hypothalamus and hippocampus, a similar developmental profile was seen: NTPDase3 gene
expression was stable during postnatal development and increased in adults. In the cortex,
upregulation of NTPDase3 mRNA expression was seen at PD15 and further increase was
evidenced in adults. Immunohistochemical analysis at PD7 revealed faint neuronal NTPDase3
localization, in dorsal hypothalamus. The immunoreactivity (ir) gradually increased in PD15
and PD20, in clusters of cells in the lateral, ventral and dorsomedial hypothalamus.
Furthermore, in PD20 animals, NTPDase3-ir was detected on short fibers in the posterior
hypothalamic area, while in PD30 the fibers appeared progressively longer and markedly
varicose. In adults, the strongest NTPDase3-ir was observed in collections of cells in
dorsomedial hypothalamic nucleus, dorsal and lateral hypothalamus and in several thalamic
areas, whereas the varicose fibers traversed entire diencephalon, particularly paraventricular
thalamic nucleus, ventromedial and dorsomedial hypothalamic nuclei, the arcuate nucleus and
the prefornical part of the lateral hypothalamus. The presumably ascending NTPDase3-ir
fibers were first observed in PD20; their density and the varicose appearance increased until
the adulthood. Prominent enhancement of NTPDase3-ir in the hypothalamus coincides with
age when animals acquire diurnal rhythms of sleeping and feeding, supporting the hypothesis
that this enzyme may be involved in regulation of homeostatic functions.",
publisher = "Amsterdam: Elsevier Science Publisher",
journal = "Journal of chemical neuroanatomy",
title = "Expression of ecto-nucleoside triphosphate diphosphohydrolase3 (NTPDase3) in the female rat brain during postnatal development",
volume = "77",
doi = "10.1016/j.jchemneu.2016.04.001",
pages = "10-18"
}

Grković, I., Bjelobaba, I., Mitrović, N., Lavrnja, I., Drakulić, D., Martinović, J., Stanojlović, M., Horvat, A.,& Nedeljković, N.. (2016). Expression of ecto-nucleoside triphosphate diphosphohydrolase3 (NTPDase3) in the female rat brain during postnatal development. in Journal of chemical neuroanatomy
Amsterdam: Elsevier Science Publisher., 77, 10-18.
https://doi.org/10.1016/j.jchemneu.2016.04.001

Grković I, Bjelobaba I, Mitrović N, Lavrnja I, Drakulić D, Martinović J, Stanojlović M, Horvat A, Nedeljković N. Expression of ecto-nucleoside triphosphate diphosphohydrolase3 (NTPDase3) in the female rat brain during postnatal development. in Journal of chemical neuroanatomy. 2016;77:10-18.
doi:10.1016/j.jchemneu.2016.04.001 .

Grković, Ivana, Bjelobaba, Ivana, Mitrović, Nataša, Lavrnja, Irena, Drakulić, Dunja, Martinović, Jelena, Stanojlović, Miloš, Horvat, Anica, Nedeljković, Nadežda, "Expression of ecto-nucleoside triphosphate diphosphohydrolase3 (NTPDase3) in the female rat brain during postnatal development" in Journal of chemical neuroanatomy, 77 (2016):10-18,
https://doi.org/10.1016/j.jchemneu.2016.04.001 . .

TY  - JOUR
AU  - Drakulic, Dunja
AU  - Stanojlovic, Milos
AU  - Nedeljkovic, Nadezda
AU  - Grkovic, Ivana
AU  - Velickovic, Natasa
AU  - Gusevac, Ivana
AU  - Mitrovic, Natasa
AU  - Buzadzic, Ivana
AU  - Horvat, Anica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1982
AB  - Although dexamethasone (DEX), a synthetic glucocorticoid receptor (GR)
   analog with profound effects on energy metabolism, immune system, and
   hypothalamic-pituitary-adrenal axis, is widely used therapeutically, its
   impact on the brain is poorly understood. The aim of the present study
   was to explore the effect of repeated low-dose DEX administration on the
   activity and expression of the ectonucleotidase enzymes which hydrolyze
   and therefore control extracellular ATP and adenosine concentrations in
   the synaptic cleft. Ectonucleotidases tested were ectonucleoside
   triphosphate diphosphohydrolase 1-3 (NTPDase1-3) and
   ecto-5'-nucleotidase (eN), whereas the effects were evaluated in two
   brain areas that show different sensitivity to glucocorticoid action,
   hippocampus, and cerebral cortex. In the hippocampus, but not in
   cerebral cortex, modest level of neurodegenerative changes as well as
   increase in ATP, ADP, and AMP hydrolysis and upregulation of NTPDase1
   and eN mRNA expression ensued under the influence of DEX. The observed
   pattern of ectonucleotidase activation, which creates tissue volume with
   enhanced capacity for adenosine formation, is the hallmark of the
   response after different insults to the brain.
T2  - Journal of Molecular Neuroscience
T1  - Upregulation of Nucleoside Triphosphate Diphosphohydrolase-1 and
 Ecto-5'-Nucleotidase in Rat Hippocampus after Repeated Low-Dose
 Dexamethasone Administration
IS  - 4
VL  - 55
DO  - 10.1007/s12031-014-0452-y
SP  - 959
EP  - 967
ER  - 

@article{
author = "Drakulic, Dunja and Stanojlovic, Milos and Nedeljkovic, Nadezda and Grkovic, Ivana and Velickovic, Natasa and Gusevac, Ivana and Mitrovic, Natasa and Buzadzic, Ivana and Horvat, Anica",
year = "2015",
abstract = "Although dexamethasone (DEX), a synthetic glucocorticoid receptor (GR)
   analog with profound effects on energy metabolism, immune system, and
   hypothalamic-pituitary-adrenal axis, is widely used therapeutically, its
   impact on the brain is poorly understood. The aim of the present study
   was to explore the effect of repeated low-dose DEX administration on the
   activity and expression of the ectonucleotidase enzymes which hydrolyze
   and therefore control extracellular ATP and adenosine concentrations in
   the synaptic cleft. Ectonucleotidases tested were ectonucleoside
   triphosphate diphosphohydrolase 1-3 (NTPDase1-3) and
   ecto-5'-nucleotidase (eN), whereas the effects were evaluated in two
   brain areas that show different sensitivity to glucocorticoid action,
   hippocampus, and cerebral cortex. In the hippocampus, but not in
   cerebral cortex, modest level of neurodegenerative changes as well as
   increase in ATP, ADP, and AMP hydrolysis and upregulation of NTPDase1
   and eN mRNA expression ensued under the influence of DEX. The observed
   pattern of ectonucleotidase activation, which creates tissue volume with
   enhanced capacity for adenosine formation, is the hallmark of the
   response after different insults to the brain.",
journal = "Journal of Molecular Neuroscience",
title = "Upregulation of Nucleoside Triphosphate Diphosphohydrolase-1 and
 Ecto-5'-Nucleotidase in Rat Hippocampus after Repeated Low-Dose
 Dexamethasone Administration",
number = "4",
volume = "55",
doi = "10.1007/s12031-014-0452-y",
pages = "959-967"
}

Drakulic, D., Stanojlovic, M., Nedeljkovic, N., Grkovic, I., Velickovic, N., Gusevac, I., Mitrovic, N., Buzadzic, I.,& Horvat, A.. (2015). Upregulation of Nucleoside Triphosphate Diphosphohydrolase-1 and
 Ecto-5'-Nucleotidase in Rat Hippocampus after Repeated Low-Dose
 Dexamethasone Administration. in Journal of Molecular Neuroscience, 55(4), 959-967.
https://doi.org/10.1007/s12031-014-0452-y

Drakulic D, Stanojlovic M, Nedeljkovic N, Grkovic I, Velickovic N, Gusevac I, Mitrovic N, Buzadzic I, Horvat A. Upregulation of Nucleoside Triphosphate Diphosphohydrolase-1 and
 Ecto-5'-Nucleotidase in Rat Hippocampus after Repeated Low-Dose
 Dexamethasone Administration. in Journal of Molecular Neuroscience. 2015;55(4):959-967.
doi:10.1007/s12031-014-0452-y .

Drakulic, Dunja, Stanojlovic, Milos, Nedeljkovic, Nadezda, Grkovic, Ivana, Velickovic, Natasa, Gusevac, Ivana, Mitrovic, Natasa, Buzadzic, Ivana, Horvat, Anica, "Upregulation of Nucleoside Triphosphate Diphosphohydrolase-1 and
 Ecto-5'-Nucleotidase in Rat Hippocampus after Repeated Low-Dose
 Dexamethasone Administration" in Journal of Molecular Neuroscience, 55, no. 4 (2015):959-967,
https://doi.org/10.1007/s12031-014-0452-y . .

TY  - JOUR
AU  - Petrovic, Snjezana
AU  - Milosevic, Maja
AU  - Ristic-Medic, Danijela
AU  - Velickovic, Natasa
AU  - Drakulic, Dunja
AU  - Grkovic, Ivana
AU  - Horvat, Anica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2077
AB  - Our earlier studies found that in vitro estradiol modulates
   mitochondrial Ca2+ transport in discrete brain regions. The present
   study examined the role of estradiol receptors (ERs) in
   estradiol-induced inhibition of Ca2+ efflux from synaptosomal
   mitochondria isolated from rat caudate nuclei and brain stems.
   Radioactively labeled CaCl2 (0.6-0.75 mu Ci (CaCl2)-Ca-45) was used for
   Ca2+ transport monitoring. The results revealed that in the presence of
   ER antagonist 7 alpha, 17 beta-{[}9{[}(4,4,5,5,5-pentafluoropentyl)
   sulfinyl] nonyl] estra-1,3,5( 10)-triene-3,17-diol (ICI 182,780) (1 mu
   mol/L), the inhibitory effect of estradiol on mitochondrial Ca2+ efflux
   was more than 60\% decreased, suggesting the involvement of ER in this
   mode of estradiol neuromodulatory action. When particular contributions
   of ER alpha and ER beta were tested, it was found that ER beta agonist
   2,3-bis(4-hydroxy phenyl)-propionitrile (10 nmol/L) inhibited Ca2+
   efflux more than 20\%, while the inhibition with ER alpha agonist 4,4',
   4''-(4-propyl-{[}1H]-pyrazole-1,3,5-triyl) trisphenol (10 nmol/L) was
   about 10\%, both compared to the control. Both agonists demonstrated
   attenuation of Ca2+ efflux decrease in the presence of mitochondrial
   Na+/Ca2+ exchanger antagonist
   7-chloro-5-(2-chlorophenyl)-1,5-dihyhdro-4,1-benzothiazepin-2(3H)-one
   (10 mu mol/L), showing interference with the inhibitory action of that
   agent. Our results strongly indicate ERs as the mediators of
   estradiol-induced mitochondrial Ca2+ efflux inhibition in rat caudate
   nucleus and brain stem synaptosomes.
T2  - Turkish Journal of Biology
T1  - Estradiol receptors mediate estradiol-induced inhibition of
 mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem
IS  - 2
VL  - 39
DO  - 10.3906/biy-1408-62
SP  - 328
EP  - 334
ER  - 

@article{
author = "Petrovic, Snjezana and Milosevic, Maja and Ristic-Medic, Danijela and Velickovic, Natasa and Drakulic, Dunja and Grkovic, Ivana and Horvat, Anica",
year = "2015",
abstract = "Our earlier studies found that in vitro estradiol modulates
   mitochondrial Ca2+ transport in discrete brain regions. The present
   study examined the role of estradiol receptors (ERs) in
   estradiol-induced inhibition of Ca2+ efflux from synaptosomal
   mitochondria isolated from rat caudate nuclei and brain stems.
   Radioactively labeled CaCl2 (0.6-0.75 mu Ci (CaCl2)-Ca-45) was used for
   Ca2+ transport monitoring. The results revealed that in the presence of
   ER antagonist 7 alpha, 17 beta-{[}9{[}(4,4,5,5,5-pentafluoropentyl)
   sulfinyl] nonyl] estra-1,3,5( 10)-triene-3,17-diol (ICI 182,780) (1 mu
   mol/L), the inhibitory effect of estradiol on mitochondrial Ca2+ efflux
   was more than 60\% decreased, suggesting the involvement of ER in this
   mode of estradiol neuromodulatory action. When particular contributions
   of ER alpha and ER beta were tested, it was found that ER beta agonist
   2,3-bis(4-hydroxy phenyl)-propionitrile (10 nmol/L) inhibited Ca2+
   efflux more than 20\%, while the inhibition with ER alpha agonist 4,4',
   4''-(4-propyl-{[}1H]-pyrazole-1,3,5-triyl) trisphenol (10 nmol/L) was
   about 10\%, both compared to the control. Both agonists demonstrated
   attenuation of Ca2+ efflux decrease in the presence of mitochondrial
   Na+/Ca2+ exchanger antagonist
   7-chloro-5-(2-chlorophenyl)-1,5-dihyhdro-4,1-benzothiazepin-2(3H)-one
   (10 mu mol/L), showing interference with the inhibitory action of that
   agent. Our results strongly indicate ERs as the mediators of
   estradiol-induced mitochondrial Ca2+ efflux inhibition in rat caudate
   nucleus and brain stem synaptosomes.",
journal = "Turkish Journal of Biology",
title = "Estradiol receptors mediate estradiol-induced inhibition of
 mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem",
number = "2",
volume = "39",
doi = "10.3906/biy-1408-62",
pages = "328-334"
}

Petrovic, S., Milosevic, M., Ristic-Medic, D., Velickovic, N., Drakulic, D., Grkovic, I.,& Horvat, A.. (2015). Estradiol receptors mediate estradiol-induced inhibition of
 mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem. in Turkish Journal of Biology, 39(2), 328-334.
https://doi.org/10.3906/biy-1408-62

Petrovic S, Milosevic M, Ristic-Medic D, Velickovic N, Drakulic D, Grkovic I, Horvat A. Estradiol receptors mediate estradiol-induced inhibition of
 mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem. in Turkish Journal of Biology. 2015;39(2):328-334.
doi:10.3906/biy-1408-62 .

Petrovic, Snjezana, Milosevic, Maja, Ristic-Medic, Danijela, Velickovic, Natasa, Drakulic, Dunja, Grkovic, Ivana, Horvat, Anica, "Estradiol receptors mediate estradiol-induced inhibition of
 mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem" in Turkish Journal of Biology, 39, no. 2 (2015):328-334,
https://doi.org/10.3906/biy-1408-62 . .

TY  - JOUR
AU  - Grkovic, Ivana
AU  - Bjelobaba, Ivana
AU  - Nedeljkovic, Nadezda
AU  - Mitrovic, Natasa
AU  - Drakulic, Dunja
AU  - Stanojlovic, Milos
AU  - Horvat, Anica
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2170
AB  - Ecto-5'-nucleotidase (e-5NT), a glycosylphosphatidylinositol-linked
   membrane protein, catalyzes a conversion of AMP to adenosine, which
   influences nearly every aspect of brain physiology, including embryonic
   and postnatal brain development. The present study aimed to investigate
   a pattern of expression, activity and kinetic properties of e-5NT in the
   hippocampal formation and synaptic plasma membrane (SPM) preparations in
   rats at postnatal days (PDs) 7, 15, 20, 30 and 90. By combining gene
   expression analysis and enzyme histochemistry, we observed that e-5NT
   mRNA reached the adult level at PD20, while the enzyme activity
   continued to increase beyond this age. Further analysis revealed that
   hippocampal layers rich in synapses expressed the highest levels of
   e-5NT activity, while in layers populated with neuronal cell bodies, the
   enzyme activity was weak or absent. Therefore, activity and expression
   of e-5NT were analyzed in SPM preparations isolated from rats at
   different ages. The presence of two protein bands of about 65 and 68 kDa
   was determined by immunoblot analysis. The 65-kDa band was present at
   all ages, and its abundance increased from PD7 to PD20. The 68-kDa band
   appeared at PD15 and increased until PD30, coinciding with the increase
   of e-5NT activity, substrate affinity and enzymatic efficiency. Since
   distinct e-5NT isoforms may derive from different patterns of the enzyme
   protein N-glycosylation, we speculate that long-term regulation of e-5NT
   activity in adulthood may be effectuated at posttranslational level and
   without overall change in the gene and protein expression.
T2  - Journal of Molecular Neuroscience
T1  - Developmental Increase in Ecto-5'-Nucleotidase Activity Overlaps with
 Appearance of Two Immunologically Distinct Enzyme Isoforms in Rat
 Hippocampal Synaptic Plasma Membranes
IS  - 1
VL  - 54
DO  - 10.1007/s12031-014-0256-0
SP  - 109
EP  - 118
ER  - 

@article{
author = "Grkovic, Ivana and Bjelobaba, Ivana and Nedeljkovic, Nadezda and Mitrovic, Natasa and Drakulic, Dunja and Stanojlovic, Milos and Horvat, Anica",
year = "2014",
abstract = "Ecto-5'-nucleotidase (e-5NT), a glycosylphosphatidylinositol-linked
   membrane protein, catalyzes a conversion of AMP to adenosine, which
   influences nearly every aspect of brain physiology, including embryonic
   and postnatal brain development. The present study aimed to investigate
   a pattern of expression, activity and kinetic properties of e-5NT in the
   hippocampal formation and synaptic plasma membrane (SPM) preparations in
   rats at postnatal days (PDs) 7, 15, 20, 30 and 90. By combining gene
   expression analysis and enzyme histochemistry, we observed that e-5NT
   mRNA reached the adult level at PD20, while the enzyme activity
   continued to increase beyond this age. Further analysis revealed that
   hippocampal layers rich in synapses expressed the highest levels of
   e-5NT activity, while in layers populated with neuronal cell bodies, the
   enzyme activity was weak or absent. Therefore, activity and expression
   of e-5NT were analyzed in SPM preparations isolated from rats at
   different ages. The presence of two protein bands of about 65 and 68 kDa
   was determined by immunoblot analysis. The 65-kDa band was present at
   all ages, and its abundance increased from PD7 to PD20. The 68-kDa band
   appeared at PD15 and increased until PD30, coinciding with the increase
   of e-5NT activity, substrate affinity and enzymatic efficiency. Since
   distinct e-5NT isoforms may derive from different patterns of the enzyme
   protein N-glycosylation, we speculate that long-term regulation of e-5NT
   activity in adulthood may be effectuated at posttranslational level and
   without overall change in the gene and protein expression.",
journal = "Journal of Molecular Neuroscience",
title = "Developmental Increase in Ecto-5'-Nucleotidase Activity Overlaps with
 Appearance of Two Immunologically Distinct Enzyme Isoforms in Rat
 Hippocampal Synaptic Plasma Membranes",
number = "1",
volume = "54",
doi = "10.1007/s12031-014-0256-0",
pages = "109-118"
}

Grkovic, I., Bjelobaba, I., Nedeljkovic, N., Mitrovic, N., Drakulic, D., Stanojlovic, M.,& Horvat, A.. (2014). Developmental Increase in Ecto-5'-Nucleotidase Activity Overlaps with
 Appearance of Two Immunologically Distinct Enzyme Isoforms in Rat
 Hippocampal Synaptic Plasma Membranes. in Journal of Molecular Neuroscience, 54(1), 109-118.
https://doi.org/10.1007/s12031-014-0256-0

Grkovic I, Bjelobaba I, Nedeljkovic N, Mitrovic N, Drakulic D, Stanojlovic M, Horvat A. Developmental Increase in Ecto-5'-Nucleotidase Activity Overlaps with
 Appearance of Two Immunologically Distinct Enzyme Isoforms in Rat
 Hippocampal Synaptic Plasma Membranes. in Journal of Molecular Neuroscience. 2014;54(1):109-118.
doi:10.1007/s12031-014-0256-0 .

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