TY  - JOUR
AU  - Popović, Dušanka
AU  - Kulaš, Jelena
AU  - Tucović, Dina
AU  - Popov Aleksandrov, Aleksandra
AU  - Malešević, Anastasija
AU  - Glamočlija, Jasmina
AU  - Brdarić, Emilija
AU  - Soković Bajić, Svetlana
AU  - Golić, Nataša
AU  - Mirkov, Ivana
AU  - Tolinački, Maja
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6084
AB  - While the effect of gut microbiota and/or inflammation on a distant body site, including the lungs (gut–lung axis), has been well characterized, data about the influence of lung microbiota and lung inflammation on gut homeostasis (lung–gut axis) are scarce. Using a well-characterized model of pulmonary infection with the fungus Aspergillus fumigatus, we investigated alterations in the lung and gut microbiota by next-generation sequencing of the V3–V4 regions of total bacterial DNA. Pulmonary inflammation due to the fungus A. fumigatus caused bacterial dysbiosis in both lungs and gut, but with different characteristics. While increased alpha diversity and unchanged bacterial composition were noted in the lungs, dysbiosis in the gut was characterized by decreased alpha diversity indices and modified bacterial composition. The altered homeostasis in the lungs allows the immigration of new bacterial species of which 41.8% were found in the feces, indicating that some degree of bacterial migration from the gut to the lungs occurs. On the contrary, the dysbiosis occurring in the gut during pulmonary infection was a consequence of the local activity of the immune system. In addition, the alteration of gut microbiota in response to pulmonary infection depends on the bacterial composition before infection, as no changes in gut bacterial microbiota were detected in a rat strain with diverse gut bacteria. The data presented support the existence of the lung–gut axis and provide additional insight into this mechanism.
PB  - American Society for Microbiology
T2  - Microbiology Spectrum
T1  - Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition
IS  - 5
VL  - 11
DO  - 10.1128/spectrum.01990-23
SP  - e0199023
ER  - 

@article{
author = "Popović, Dušanka and Kulaš, Jelena and Tucović, Dina and Popov Aleksandrov, Aleksandra and Malešević, Anastasija and Glamočlija, Jasmina and Brdarić, Emilija and Soković Bajić, Svetlana and Golić, Nataša and Mirkov, Ivana and Tolinački, Maja",
year = "2023",
abstract = "While the effect of gut microbiota and/or inflammation on a distant body site, including the lungs (gut–lung axis), has been well characterized, data about the influence of lung microbiota and lung inflammation on gut homeostasis (lung–gut axis) are scarce. Using a well-characterized model of pulmonary infection with the fungus Aspergillus fumigatus, we investigated alterations in the lung and gut microbiota by next-generation sequencing of the V3–V4 regions of total bacterial DNA. Pulmonary inflammation due to the fungus A. fumigatus caused bacterial dysbiosis in both lungs and gut, but with different characteristics. While increased alpha diversity and unchanged bacterial composition were noted in the lungs, dysbiosis in the gut was characterized by decreased alpha diversity indices and modified bacterial composition. The altered homeostasis in the lungs allows the immigration of new bacterial species of which 41.8% were found in the feces, indicating that some degree of bacterial migration from the gut to the lungs occurs. On the contrary, the dysbiosis occurring in the gut during pulmonary infection was a consequence of the local activity of the immune system. In addition, the alteration of gut microbiota in response to pulmonary infection depends on the bacterial composition before infection, as no changes in gut bacterial microbiota were detected in a rat strain with diverse gut bacteria. The data presented support the existence of the lung–gut axis and provide additional insight into this mechanism.",
publisher = "American Society for Microbiology",
journal = "Microbiology Spectrum",
title = "Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition",
number = "5",
volume = "11",
doi = "10.1128/spectrum.01990-23",
pages = "e0199023"
}

Popović, D., Kulaš, J., Tucović, D., Popov Aleksandrov, A., Malešević, A., Glamočlija, J., Brdarić, E., Soković Bajić, S., Golić, N., Mirkov, I.,& Tolinački, M.. (2023). Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition. in Microbiology Spectrum
American Society for Microbiology., 11(5), e0199023.
https://doi.org/10.1128/spectrum.01990-23

Popović D, Kulaš J, Tucović D, Popov Aleksandrov A, Malešević A, Glamočlija J, Brdarić E, Soković Bajić S, Golić N, Mirkov I, Tolinački M. Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition. in Microbiology Spectrum. 2023;11(5):e0199023.
doi:10.1128/spectrum.01990-23 .

Popović, Dušanka, Kulaš, Jelena, Tucović, Dina, Popov Aleksandrov, Aleksandra, Malešević, Anastasija, Glamočlija, Jasmina, Brdarić, Emilija, Soković Bajić, Svetlana, Golić, Nataša, Mirkov, Ivana, Tolinački, Maja, "Gut microbial dysbiosis occurring during pulmonary fungal infection in rats is linked to inflammation and depends on healthy microbiota composition" in Microbiology Spectrum, 11, no. 5 (2023):e0199023,
https://doi.org/10.1128/spectrum.01990-23 . .

TY  - CONF
AU  - Brdarić, Emilija
AU  - Popović, Dušanka
AU  - Kulaš, Jelena
AU  - Soković Bajić, Svetlana
AU  - Golić, Nataša
AU  - Mirkov, Ivana
AU  - Živković, Milica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6113
AB  - Crevna mikrobiota ima važnu ulogu u održavanju homeostaze koju ostvaruje preko interakcija sa imunskim
sistemom i gastrointestinalnim traktom. Toksične supstance ostvaruju direktan štetni efekat na sastav
i funkcionalnost crevne mikrobiote. Kadmijum (Cd) je metal sa izraženom toksičnošću, koji se povezuje
sa brojnim oboljenjima. U našoj studiji je predložen mehanizam zaštite koji bi se ostvario posredstvom
bakterijskih egzopolisaharida (EPS) izolovanih iz soja Lactiplantibacillus plantarum BGAN8, a bazira se
na visokom afinitetu adsorpcije kadmijumovih jona. Ciljevi rada su bili utvrđivanje efekata produženog
delovanja kadmijuma na sastav crevne mikrobiote Dark agouti (DA) pacova, kao i izučavanje efekta
EPS-AN8 na ublažavanje takvih štetnih efekata.
Pacovi su kroz vodu unosili nižu (5 ppm) i višu (50 ppm) dozu kadmijuma, dok je EPS-AN8 (100 μg/ml) dat
kroz hranu za životinje. Totalna DNK je izolovana iz lumena duodenuma i 16S rDNK amplikon je sekvenciran
strategijom uparenih krajeva na Illumina NovaSeq platformi. Dodatno, praćene su promene u
inflamaciji i oksidativnom stresu homogenata duodenuma. Kadmijum je favorizovao rast oportunističkih
patogenih bakterija poput pripadnika rodova Blautia, Prevotella, Alloprevotela, Bacteroides, dok je
relativna zastupljenost laktobacila bila značajno smanjena. Obe doze kadmijuma su stimulisale produkciju
proinflamatornih citokina (IL-1β, TNF-α i IFN-γ) i povisile nivo oksidativnog stresa (MDA, CAT i
GST). Oralni unos EPS-AN8 je značajno ublažio sve navedene promene. Oralna izloženost kadmijumu
uzrokuje nedvosmislene promene u sastavu mikrobiote, koje mogu biti praćene i pojačanim nivoima
inflamacije i oksidativnog stresa. Nasuprot tome, istovremeni unos EPS-AN8 redukuje pojavu takvih
promena.
AB  - The gut microbiota has important role in maintaining homeostasis via its effect on the immune system
and healthy gastrointestinal tract. Environmental toxicants directly influenced the changes in gut microbiota
composition. Cadmium (Cd) is a hazard, world widely distributed metal. We have proposed
the putative mechanism of protection against Cd harmful effect in the organism by using exopolysaccharides
derived from Lactiplantibacillus plantarum BGAN8 (EPS-AN8) which has great ability to sequester Cd ions.
Aims of our study were to examine Cd related changes in the gut microbiota of Dark agouti (DA) rats after
prolonged oral exposure (30 days) to this metal and to determine impact of EPS-AN8 in preventing
those changes.
Rats were given via water lower (5 ppm) and higher (50 ppm) Cd’s dose. In paralel, EPS-AN8 was administrated
through food (100 μg/ml). Total DNA from duodenum was isolated and PCR amplicon for 16SrRNA was
sequenced on Illumina NovaSeq paired end platform. Moreover, we have followed changes in
oxidative stress and inflammation in duodenum. Cd favored growth of opportunistic pathogen bacteria
genera (Blautia, Prevotella, Alloprevotela, Bacteroides) while the relative abundance of lactobacilli was
decreased. The both doses of Cd increased production of inflammatory cytokines (IL-1β, TNF-α, and
IFN-γ). Also, parameters of oxidative stress were significantly upregulated (MDA, CAT, and GST).
All the mentioned changes were alleviated by EPS-AN8. Oral exposure to Cd lead to inevitable changes
in the microbiota structure followed by inflammation and oxidative stress of surrounding tissue, while
administration of EPS-AN8 reversed those changes.
PB  - Belgrade: Serbian Society of Toxicology
C3  - Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia
T1  - Gut microbiota as a target of toxic compounds
T1  - Crevna mikrobiota kao osetljiva meta delovanja toksičnih supstanci
SP  - 92
EP  - 93
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6113
ER  - 

@conference{
author = "Brdarić, Emilija and Popović, Dušanka and Kulaš, Jelena and Soković Bajić, Svetlana and Golić, Nataša and Mirkov, Ivana and Živković, Milica",
year = "2023",
abstract = "Crevna mikrobiota ima važnu ulogu u održavanju homeostaze koju ostvaruje preko interakcija sa imunskim
sistemom i gastrointestinalnim traktom. Toksične supstance ostvaruju direktan štetni efekat na sastav
i funkcionalnost crevne mikrobiote. Kadmijum (Cd) je metal sa izraženom toksičnošću, koji se povezuje
sa brojnim oboljenjima. U našoj studiji je predložen mehanizam zaštite koji bi se ostvario posredstvom
bakterijskih egzopolisaharida (EPS) izolovanih iz soja Lactiplantibacillus plantarum BGAN8, a bazira se
na visokom afinitetu adsorpcije kadmijumovih jona. Ciljevi rada su bili utvrđivanje efekata produženog
delovanja kadmijuma na sastav crevne mikrobiote Dark agouti (DA) pacova, kao i izučavanje efekta
EPS-AN8 na ublažavanje takvih štetnih efekata.
Pacovi su kroz vodu unosili nižu (5 ppm) i višu (50 ppm) dozu kadmijuma, dok je EPS-AN8 (100 μg/ml) dat
kroz hranu za životinje. Totalna DNK je izolovana iz lumena duodenuma i 16S rDNK amplikon je sekvenciran
strategijom uparenih krajeva na Illumina NovaSeq platformi. Dodatno, praćene su promene u
inflamaciji i oksidativnom stresu homogenata duodenuma. Kadmijum je favorizovao rast oportunističkih
patogenih bakterija poput pripadnika rodova Blautia, Prevotella, Alloprevotela, Bacteroides, dok je
relativna zastupljenost laktobacila bila značajno smanjena. Obe doze kadmijuma su stimulisale produkciju
proinflamatornih citokina (IL-1β, TNF-α i IFN-γ) i povisile nivo oksidativnog stresa (MDA, CAT i
GST). Oralni unos EPS-AN8 je značajno ublažio sve navedene promene. Oralna izloženost kadmijumu
uzrokuje nedvosmislene promene u sastavu mikrobiote, koje mogu biti praćene i pojačanim nivoima
inflamacije i oksidativnog stresa. Nasuprot tome, istovremeni unos EPS-AN8 redukuje pojavu takvih
promena., The gut microbiota has important role in maintaining homeostasis via its effect on the immune system
and healthy gastrointestinal tract. Environmental toxicants directly influenced the changes in gut microbiota
composition. Cadmium (Cd) is a hazard, world widely distributed metal. We have proposed
the putative mechanism of protection against Cd harmful effect in the organism by using exopolysaccharides
derived from Lactiplantibacillus plantarum BGAN8 (EPS-AN8) which has great ability to sequester Cd ions.
Aims of our study were to examine Cd related changes in the gut microbiota of Dark agouti (DA) rats after
prolonged oral exposure (30 days) to this metal and to determine impact of EPS-AN8 in preventing
those changes.
Rats were given via water lower (5 ppm) and higher (50 ppm) Cd’s dose. In paralel, EPS-AN8 was administrated
through food (100 μg/ml). Total DNA from duodenum was isolated and PCR amplicon for 16SrRNA was
sequenced on Illumina NovaSeq paired end platform. Moreover, we have followed changes in
oxidative stress and inflammation in duodenum. Cd favored growth of opportunistic pathogen bacteria
genera (Blautia, Prevotella, Alloprevotela, Bacteroides) while the relative abundance of lactobacilli was
decreased. The both doses of Cd increased production of inflammatory cytokines (IL-1β, TNF-α, and
IFN-γ). Also, parameters of oxidative stress were significantly upregulated (MDA, CAT, and GST).
All the mentioned changes were alleviated by EPS-AN8. Oral exposure to Cd lead to inevitable changes
in the microbiota structure followed by inflammation and oxidative stress of surrounding tissue, while
administration of EPS-AN8 reversed those changes.",
publisher = "Belgrade: Serbian Society of Toxicology",
journal = "Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia",
title = "Gut microbiota as a target of toxic compounds, Crevna mikrobiota kao osetljiva meta delovanja toksičnih supstanci",
pages = "92-93",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6113"
}

Brdarić, E., Popović, D., Kulaš, J., Soković Bajić, S., Golić, N., Mirkov, I.,& Živković, M.. (2023). Gut microbiota as a target of toxic compounds. in Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia
Belgrade: Serbian Society of Toxicology., 92-93.
https://hdl.handle.net/21.15107/rcub_ibiss_6113

Brdarić E, Popović D, Kulaš J, Soković Bajić S, Golić N, Mirkov I, Živković M. Gut microbiota as a target of toxic compounds. in Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia. 2023;:92-93.
https://hdl.handle.net/21.15107/rcub_ibiss_6113 .

Brdarić, Emilija, Popović, Dušanka, Kulaš, Jelena, Soković Bajić, Svetlana, Golić, Nataša, Mirkov, Ivana, Živković, Milica, "Gut microbiota as a target of toxic compounds" in Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia (2023):92-93,
https://hdl.handle.net/21.15107/rcub_ibiss_6113 .

TY  - CONF
AU  - Popović, Dušanka
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Malešević, Anastasija
AU  - Glamočlija, Jasmina
AU  - Soković Bajić, Svetlana
AU  - Brdarić, Emilija
AU  - Tolinački, Maja
AU  - Golić, Nataša
PY  - 2023
UR  - https://probiotics-prebiotics-newfood.com/wp-content/uploads/2023/09/Pogramme-web-07_09_2023.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6166
AB  - Objective:
The little data of the effect of pulmonary fungal infection on lung and
gut microbiota exist, thus aim of our study was to examine the effect of
Aspergillus fumigatus infection on bacterial homestasis in these organs in
Dark Agouti (DA) and Albino Oxford (AO) rats known to develop different
immune responce to the fungus.
Methods:
16S rRNA amplicon sequencing of the V3-V4 hypervariable region.
Enzyme-linked immunosorbent assay for cytokine content (IL-10, IL-6, TNF,
IL-1beta, IL-17 i IFN-gamma) in lung and gut homogenats, and plasma (IL-
6, IL-17, TNF). Myeloperoxidase, catalase, glutathione S-transferase and
content of reduced glutathione in lung homogenates.
Results:
No initial differences in lung bacterial microbiota between healthy DA
and AO rats exists, but A. fumigatus infection resulted in increase in
alpha diversity (without effect on bacterial composition) in both strains.
Changes in relative abundance at genus and species level were expressed
differently (increased in DA, decreased in AO). Dysbiosis in lung coincided
with increase of IFN-gamma, IL-17 and MPO in both strains, and increase
oxidative stress solely in DA. Gut inflammation was noted in both strains,
while bacterial dysbiosis only in DA.
Conclusions:
Pulmonary fungal infection resulted in bacterial dysbiosis in lung (in both
rat strains), and gut (solely in DA). Noted lung and gut inflammation in
these two rat strains, coexisted with different effects on bacterial
homeostasis, which could be due to different genetic background or
bacterial composition prior infection.
PB  - Roma: EMEC
C3  - 12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy
T1  - The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats
SP  - 90
EP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6166
ER  - 

@conference{
author = "Popović, Dušanka and Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Tucović, Dina and Kulaš, Jelena and Malešević, Anastasija and Glamočlija, Jasmina and Soković Bajić, Svetlana and Brdarić, Emilija and Tolinački, Maja and Golić, Nataša",
year = "2023",
abstract = "Objective:
The little data of the effect of pulmonary fungal infection on lung and
gut microbiota exist, thus aim of our study was to examine the effect of
Aspergillus fumigatus infection on bacterial homestasis in these organs in
Dark Agouti (DA) and Albino Oxford (AO) rats known to develop different
immune responce to the fungus.
Methods:
16S rRNA amplicon sequencing of the V3-V4 hypervariable region.
Enzyme-linked immunosorbent assay for cytokine content (IL-10, IL-6, TNF,
IL-1beta, IL-17 i IFN-gamma) in lung and gut homogenats, and plasma (IL-
6, IL-17, TNF). Myeloperoxidase, catalase, glutathione S-transferase and
content of reduced glutathione in lung homogenates.
Results:
No initial differences in lung bacterial microbiota between healthy DA
and AO rats exists, but A. fumigatus infection resulted in increase in
alpha diversity (without effect on bacterial composition) in both strains.
Changes in relative abundance at genus and species level were expressed
differently (increased in DA, decreased in AO). Dysbiosis in lung coincided
with increase of IFN-gamma, IL-17 and MPO in both strains, and increase
oxidative stress solely in DA. Gut inflammation was noted in both strains,
while bacterial dysbiosis only in DA.
Conclusions:
Pulmonary fungal infection resulted in bacterial dysbiosis in lung (in both
rat strains), and gut (solely in DA). Noted lung and gut inflammation in
these two rat strains, coexisted with different effects on bacterial
homeostasis, which could be due to different genetic background or
bacterial composition prior infection.",
publisher = "Roma: EMEC",
journal = "12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy",
title = "The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats",
pages = "90-90",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6166"
}

Popović, D., Mirkov, I., Popov Aleksandrov, A., Tucović, D., Kulaš, J., Malešević, A., Glamočlija, J., Soković Bajić, S., Brdarić, E., Tolinački, M.,& Golić, N.. (2023). The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats. in 12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy
Roma: EMEC., 90-90.
https://hdl.handle.net/21.15107/rcub_ibiss_6166

Popović D, Mirkov I, Popov Aleksandrov A, Tucović D, Kulaš J, Malešević A, Glamočlija J, Soković Bajić S, Brdarić E, Tolinački M, Golić N. The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats. in 12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy. 2023;:90-90.
https://hdl.handle.net/21.15107/rcub_ibiss_6166 .

Popović, Dušanka, Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Tucović, Dina, Kulaš, Jelena, Malešević, Anastasija, Glamočlija, Jasmina, Soković Bajić, Svetlana, Brdarić, Emilija, Tolinački, Maja, Golić, Nataša, "The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats" in 12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy (2023):90-90,
https://hdl.handle.net/21.15107/rcub_ibiss_6166 .