TY  - JOUR
AU  - Popović, Dušanka
AU  - Kulaš, Jelena
AU  - Tucović, Dina
AU  - Popov Aleksandrov, Aleksandra
AU  - Glamočlija, Jasmina
AU  - Soković Bajić, Svetlana
AU  - Tolinacki, Maja
AU  - Golić, Nataša
AU  - Mirkov, Ivana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6162
AB  - Since the realization that the lungs are not sterile but are normally inhabited by various bacterial species, studies have been conducted to define healthy lung microbiota and to investigate whether it changes during lung diseases, infections, and inflammation. Using next-generation sequencing, we investigated
bacterial microbiota from whole lungs in two rat strains (previously shown to differ in gut microbiota
composition) in a healthy state and during pulmonary infection caused by the opportunistic fungus
Aspergillus fumigatus. No differences in alpha diversity indices and microbial composition between DA
and AO rats before infection were noted. Fungal infection caused dysbiosis in both rat strains, characterized
by increased alpha diversity indices and unchanged beta diversity. The relative abundance of
genera and species was increased in DA but decreased in AO rats during infection. Changes in lung
microbiota coincided with inflammation (in both rat strains) and oxidative stress (in DA rats). Disparate
response of lung microbiota in DA and AO rats to pulmonary fungal infection might render these two rat
strains differentially susceptible to a subsequent inflammatory insult.
PB  - Paris: Elsevier Masson SAS
T2  - Microbes and Infection
T1  - Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats
DO  - 10.1016/j.micinf.2023.105186
SP  - 105186
ER  - 

@article{
author = "Popović, Dušanka and Kulaš, Jelena and Tucović, Dina and Popov Aleksandrov, Aleksandra and Glamočlija, Jasmina and Soković Bajić, Svetlana and Tolinacki, Maja and Golić, Nataša and Mirkov, Ivana",
year = "2023",
abstract = "Since the realization that the lungs are not sterile but are normally inhabited by various bacterial species, studies have been conducted to define healthy lung microbiota and to investigate whether it changes during lung diseases, infections, and inflammation. Using next-generation sequencing, we investigated
bacterial microbiota from whole lungs in two rat strains (previously shown to differ in gut microbiota
composition) in a healthy state and during pulmonary infection caused by the opportunistic fungus
Aspergillus fumigatus. No differences in alpha diversity indices and microbial composition between DA
and AO rats before infection were noted. Fungal infection caused dysbiosis in both rat strains, characterized
by increased alpha diversity indices and unchanged beta diversity. The relative abundance of
genera and species was increased in DA but decreased in AO rats during infection. Changes in lung
microbiota coincided with inflammation (in both rat strains) and oxidative stress (in DA rats). Disparate
response of lung microbiota in DA and AO rats to pulmonary fungal infection might render these two rat
strains differentially susceptible to a subsequent inflammatory insult.",
publisher = "Paris: Elsevier Masson SAS",
journal = "Microbes and Infection",
title = "Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats",
doi = "10.1016/j.micinf.2023.105186",
pages = "105186"
}

Popović, D., Kulaš, J., Tucović, D., Popov Aleksandrov, A., Glamočlija, J., Soković Bajić, S., Tolinacki, M., Golić, N.,& Mirkov, I.. (2023). Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats. in Microbes and Infection
Paris: Elsevier Masson SAS., 105186.
https://doi.org/10.1016/j.micinf.2023.105186

Popović D, Kulaš J, Tucović D, Popov Aleksandrov A, Glamočlija J, Soković Bajić S, Tolinacki M, Golić N, Mirkov I. Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats. in Microbes and Infection. 2023;:105186.
doi:10.1016/j.micinf.2023.105186 .

Popović, Dušanka, Kulaš, Jelena, Tucović, Dina, Popov Aleksandrov, Aleksandra, Glamočlija, Jasmina, Soković Bajić, Svetlana, Tolinacki, Maja, Golić, Nataša, Mirkov, Ivana, "Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats" in Microbes and Infection (2023):105186,
https://doi.org/10.1016/j.micinf.2023.105186 . .

TY  - CONF
AU  - Popović, Dušanka
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Malešević, Anastasija
AU  - Glamočlija, Jasmina
AU  - Soković Bajić, Svetlana
AU  - Brdarić, Emilija
AU  - Tolinački, Maja
AU  - Golić, Nataša
PY  - 2023
UR  - https://probiotics-prebiotics-newfood.com/wp-content/uploads/2023/09/Pogramme-web-07_09_2023.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6166
AB  - Objective:
The little data of the effect of pulmonary fungal infection on lung and
gut microbiota exist, thus aim of our study was to examine the effect of
Aspergillus fumigatus infection on bacterial homestasis in these organs in
Dark Agouti (DA) and Albino Oxford (AO) rats known to develop different
immune responce to the fungus.
Methods:
16S rRNA amplicon sequencing of the V3-V4 hypervariable region.
Enzyme-linked immunosorbent assay for cytokine content (IL-10, IL-6, TNF,
IL-1beta, IL-17 i IFN-gamma) in lung and gut homogenats, and plasma (IL-
6, IL-17, TNF). Myeloperoxidase, catalase, glutathione S-transferase and
content of reduced glutathione in lung homogenates.
Results:
No initial differences in lung bacterial microbiota between healthy DA
and AO rats exists, but A. fumigatus infection resulted in increase in
alpha diversity (without effect on bacterial composition) in both strains.
Changes in relative abundance at genus and species level were expressed
differently (increased in DA, decreased in AO). Dysbiosis in lung coincided
with increase of IFN-gamma, IL-17 and MPO in both strains, and increase
oxidative stress solely in DA. Gut inflammation was noted in both strains,
while bacterial dysbiosis only in DA.
Conclusions:
Pulmonary fungal infection resulted in bacterial dysbiosis in lung (in both
rat strains), and gut (solely in DA). Noted lung and gut inflammation in
these two rat strains, coexisted with different effects on bacterial
homeostasis, which could be due to different genetic background or
bacterial composition prior infection.
PB  - Roma: EMEC
C3  - 12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy
T1  - The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats
SP  - 90
EP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6166
ER  - 

@conference{
author = "Popović, Dušanka and Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Tucović, Dina and Kulaš, Jelena and Malešević, Anastasija and Glamočlija, Jasmina and Soković Bajić, Svetlana and Brdarić, Emilija and Tolinački, Maja and Golić, Nataša",
year = "2023",
abstract = "Objective:
The little data of the effect of pulmonary fungal infection on lung and
gut microbiota exist, thus aim of our study was to examine the effect of
Aspergillus fumigatus infection on bacterial homestasis in these organs in
Dark Agouti (DA) and Albino Oxford (AO) rats known to develop different
immune responce to the fungus.
Methods:
16S rRNA amplicon sequencing of the V3-V4 hypervariable region.
Enzyme-linked immunosorbent assay for cytokine content (IL-10, IL-6, TNF,
IL-1beta, IL-17 i IFN-gamma) in lung and gut homogenats, and plasma (IL-
6, IL-17, TNF). Myeloperoxidase, catalase, glutathione S-transferase and
content of reduced glutathione in lung homogenates.
Results:
No initial differences in lung bacterial microbiota between healthy DA
and AO rats exists, but A. fumigatus infection resulted in increase in
alpha diversity (without effect on bacterial composition) in both strains.
Changes in relative abundance at genus and species level were expressed
differently (increased in DA, decreased in AO). Dysbiosis in lung coincided
with increase of IFN-gamma, IL-17 and MPO in both strains, and increase
oxidative stress solely in DA. Gut inflammation was noted in both strains,
while bacterial dysbiosis only in DA.
Conclusions:
Pulmonary fungal infection resulted in bacterial dysbiosis in lung (in both
rat strains), and gut (solely in DA). Noted lung and gut inflammation in
these two rat strains, coexisted with different effects on bacterial
homeostasis, which could be due to different genetic background or
bacterial composition prior infection.",
publisher = "Roma: EMEC",
journal = "12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy",
title = "The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats",
pages = "90-90",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6166"
}

Popović, D., Mirkov, I., Popov Aleksandrov, A., Tucović, D., Kulaš, J., Malešević, A., Glamočlija, J., Soković Bajić, S., Brdarić, E., Tolinački, M.,& Golić, N.. (2023). The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats. in 12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy
Roma: EMEC., 90-90.
https://hdl.handle.net/21.15107/rcub_ibiss_6166

Popović D, Mirkov I, Popov Aleksandrov A, Tucović D, Kulaš J, Malešević A, Glamočlija J, Soković Bajić S, Brdarić E, Tolinački M, Golić N. The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats. in 12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy. 2023;:90-90.
https://hdl.handle.net/21.15107/rcub_ibiss_6166 .

Popović, Dušanka, Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Tucović, Dina, Kulaš, Jelena, Malešević, Anastasija, Glamočlija, Jasmina, Soković Bajić, Svetlana, Brdarić, Emilija, Tolinački, Maja, Golić, Nataša, "The effect of pulmonary infection with Aspergillus fumigatus on lung and gut microbiota in Dark Agouti and Albino Oxford rats" in 12th Probiotics, Prebiotics and New Foods: Nutraceuticals, Botanicals and Phytochemicals for Nutrition and Human, ANimal and Microbiota Health: 3rd Science and Business Symposium; 2023  Sep 16-19; Rome, Italy (2023):90-90,
https://hdl.handle.net/21.15107/rcub_ibiss_6166 .