TY  - CONF
AU  - Vujošević, Mladen
AU  - Rajičić, Marija
AU  - Budinski, Ivana
AU  - Bajić, Branka
AU  - Adnađević, Tanja
AU  - Blagojević, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6429
AB  - B chromosomes (Bs) are uncommon in mammals, featuring less than 2% of species, but six out of 22 species possess them in the genus Apodemus. In Serbia, over 40 populations of yellow-necked mice
(Apodemus flavicollis) with varying habitat quality were studied. The frequency of B carriers (range: 0.09 to 0.67) increased with elevation, correlated with sub-zero days, and inversely with average temperature. B carrier frequency remained stable over eight years despite population density fluctuations, while seasonal variations linked to population size were observed. Overcrowding stress reduced B carriers among pre-reproductive individuals but enhanced survival in suboptimal conditions.
Phenotypic traits and B frequency were correlated, influencing cranial morphometric development. B carriers showed distinct developmental pathways for cranial traits, suggesting environment-specific benefits. Bs’ presence did not affect carrier fecundity or fertility and was evenly distributed across age groups. B chromosomes (up to five) displayed common structures across populations in Serbia and Eastern Europe, possibly originating from sex chromosome pericentromeric regions. B-specific chromatin spatially resembled pericentromeric sex chromosomes, suggesting a similar mechanism to bypass meiotic
checkpoints.
Current data support a heterotic model in A. flavicollis. Bs likely contribute to species adaptability by increasing genetic variability, potentially expanding their distribution.
PB  - Springer Nature
C3  - Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia
T1  - B chromosomes in populations of Apodemus flavicollis – never ending story
DO  - 10.1186/s12919-023-00284-9
SP  - 33
ER  - 

@conference{
author = "Vujošević, Mladen and Rajičić, Marija and Budinski, Ivana and Bajić, Branka and Adnađević, Tanja and Blagojević, Jelena",
year = "2023",
abstract = "B chromosomes (Bs) are uncommon in mammals, featuring less than 2% of species, but six out of 22 species possess them in the genus Apodemus. In Serbia, over 40 populations of yellow-necked mice
(Apodemus flavicollis) with varying habitat quality were studied. The frequency of B carriers (range: 0.09 to 0.67) increased with elevation, correlated with sub-zero days, and inversely with average temperature. B carrier frequency remained stable over eight years despite population density fluctuations, while seasonal variations linked to population size were observed. Overcrowding stress reduced B carriers among pre-reproductive individuals but enhanced survival in suboptimal conditions.
Phenotypic traits and B frequency were correlated, influencing cranial morphometric development. B carriers showed distinct developmental pathways for cranial traits, suggesting environment-specific benefits. Bs’ presence did not affect carrier fecundity or fertility and was evenly distributed across age groups. B chromosomes (up to five) displayed common structures across populations in Serbia and Eastern Europe, possibly originating from sex chromosome pericentromeric regions. B-specific chromatin spatially resembled pericentromeric sex chromosomes, suggesting a similar mechanism to bypass meiotic
checkpoints.
Current data support a heterotic model in A. flavicollis. Bs likely contribute to species adaptability by increasing genetic variability, potentially expanding their distribution.",
publisher = "Springer Nature",
journal = "Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia",
title = "B chromosomes in populations of Apodemus flavicollis – never ending story",
doi = "10.1186/s12919-023-00284-9",
pages = "33"
}

Vujošević, M., Rajičić, M., Budinski, I., Bajić, B., Adnađević, T.,& Blagojević, J.. (2023). B chromosomes in populations of Apodemus flavicollis – never ending story. in Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia
Springer Nature., 33.
https://doi.org/10.1186/s12919-023-00284-9

Vujošević M, Rajičić M, Budinski I, Bajić B, Adnađević T, Blagojević J. B chromosomes in populations of Apodemus flavicollis – never ending story. in Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia. 2023;:33.
doi:10.1186/s12919-023-00284-9 .

Vujošević, Mladen, Rajičić, Marija, Budinski, Ivana, Bajić, Branka, Adnađević, Tanja, Blagojević, Jelena, "B chromosomes in populations of Apodemus flavicollis – never ending story" in Meeting Abstracts from the 5th B Chromosome Conference; 2023 Oct 14-17; Petnica, Serbia (2023):33,
https://doi.org/10.1186/s12919-023-00284-9 . .

TY  - CONF
AU  - Blagojević, Jelena
AU  - Rajičić, Marija
AU  - Adnađević, Tanja
AU  - Budinski, Ivana
AU  - Bajić, Branka
AU  - Miljević, Milan
AU  - Rončević, Aleksa
AU  - Vujošević, Mladen
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5775
AB  - B chromosomes (Bs), or supernumerary chromosomes, are highly polymorphic special chromosome group. Among rodents, 37 species harbour individuals with Bs in their populations. Genus Apodemus is specific, with even six species having Bs. Furthermore, long-term studies in natural populations of the yellow-necked wood mouse Apodemus flavicollis showed that one-third of individuals carry Bs on average. In more than 40 natural populations we analysed in Serbia, the frequency of animals with Bs ranged from 0.11 to 0.63 and correlated negatively with average temperature and positively with attitude. In general, non-Mendelian transmission and frequent heterochromatic composition are characteristics that have classified Bs as selfish genetic elements in the past. However, there are increasing confirmations of active genes in them, which opens the discussion on their adaptive significance. We aim to investigate how urbanisation influences the frequency of Bs in this species. Urbanisation is rapidly changing natural habitats and affecting animal community composition, genetic diversity, and the interrelationship between populations of native species. Habitat fragmentation is the leading course of genetic diversity loss. We analysed the frequency of animals with Bs in four managed city forests on the territory of Belgrade, the capital of Serbia, characterised by different sizes and degrees of isolation. Our study revealed that the frequency of animals with Bs varies significantly among studied sites, from 0.04 to 0.50. We assume that, besides other factors, genetic drift plays a significant role in obtained differences.
PB  - Valladolid: Escuela Técnica Superior de Ingenierías Agrarias, Universidad de Valladolid
C3  - Book of Abstracts: 17th Rodens et spatium; 2023 May 22-26; Valladolid, Spain
T1  - B chromosomes in urban populations of yellow-necked wood mice, Apodemus flavicolis (Mammalia, Rodentia)
SP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5775
ER  - 

@conference{
editor = "Herrero, Cófreces, Larena, Silvia, Larena, Luque, José, Juan",
author = "Blagojević, Jelena and Rajičić, Marija and Adnađević, Tanja and Budinski, Ivana and Bajić, Branka and Miljević, Milan and Rončević, Aleksa and Vujošević, Mladen",
year = "2023",
abstract = "B chromosomes (Bs), or supernumerary chromosomes, are highly polymorphic special chromosome group. Among rodents, 37 species harbour individuals with Bs in their populations. Genus Apodemus is specific, with even six species having Bs. Furthermore, long-term studies in natural populations of the yellow-necked wood mouse Apodemus flavicollis showed that one-third of individuals carry Bs on average. In more than 40 natural populations we analysed in Serbia, the frequency of animals with Bs ranged from 0.11 to 0.63 and correlated negatively with average temperature and positively with attitude. In general, non-Mendelian transmission and frequent heterochromatic composition are characteristics that have classified Bs as selfish genetic elements in the past. However, there are increasing confirmations of active genes in them, which opens the discussion on their adaptive significance. We aim to investigate how urbanisation influences the frequency of Bs in this species. Urbanisation is rapidly changing natural habitats and affecting animal community composition, genetic diversity, and the interrelationship between populations of native species. Habitat fragmentation is the leading course of genetic diversity loss. We analysed the frequency of animals with Bs in four managed city forests on the territory of Belgrade, the capital of Serbia, characterised by different sizes and degrees of isolation. Our study revealed that the frequency of animals with Bs varies significantly among studied sites, from 0.04 to 0.50. We assume that, besides other factors, genetic drift plays a significant role in obtained differences.",
publisher = "Valladolid: Escuela Técnica Superior de Ingenierías Agrarias, Universidad de Valladolid",
journal = "Book of Abstracts: 17th Rodens et spatium; 2023 May 22-26; Valladolid, Spain",
title = "B chromosomes in urban populations of yellow-necked wood mice, Apodemus flavicolis (Mammalia, Rodentia)",
pages = "30",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5775"
}

Herrero, C., Larena, S., Larena, L., José, J., Blagojević, J., Rajičić, M., Adnađević, T., Budinski, I., Bajić, B., Miljević, M., Rončević, A.,& Vujošević, M.. (2023). B chromosomes in urban populations of yellow-necked wood mice, Apodemus flavicolis (Mammalia, Rodentia). in Book of Abstracts: 17th Rodens et spatium; 2023 May 22-26; Valladolid, Spain
Valladolid: Escuela Técnica Superior de Ingenierías Agrarias, Universidad de Valladolid., 30.
https://hdl.handle.net/21.15107/rcub_ibiss_5775

Herrero C, Larena S, Larena L, José J, Blagojević J, Rajičić M, Adnađević T, Budinski I, Bajić B, Miljević M, Rončević A, Vujošević M. B chromosomes in urban populations of yellow-necked wood mice, Apodemus flavicolis (Mammalia, Rodentia). in Book of Abstracts: 17th Rodens et spatium; 2023 May 22-26; Valladolid, Spain. 2023;:30.
https://hdl.handle.net/21.15107/rcub_ibiss_5775 .

Herrero, Cófreces, Larena, Silvia, Larena, Luque, José, Juan, Blagojević, Jelena, Rajičić, Marija, Adnađević, Tanja, Budinski, Ivana, Bajić, Branka, Miljević, Milan, Rončević, Aleksa, Vujošević, Mladen, "B chromosomes in urban populations of yellow-necked wood mice, Apodemus flavicolis (Mammalia, Rodentia)" in Book of Abstracts: 17th Rodens et spatium; 2023 May 22-26; Valladolid, Spain (2023):30,
https://hdl.handle.net/21.15107/rcub_ibiss_5775 .

TY  - CONF
AU  - Miljević, Milan
AU  - Bajić, Branka
AU  - Rajičić, Marija
AU  - Budinski, Ivana
AU  - Adnađević, Tanja
AU  - Blagojević, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4992
AB  - Many species of tapeworms of the family Taeniidae are of great importance for human and veterinary medicine, while rodents are important intermediate hosts for some of the species. The role of rodents in the tapeworm’s transmission dynamics depends on various external and internal factors. This study aimed to determine the presence of larval forms of tapeworms in rodents and molecular detection of the species, as well as to examine the relationship between different morphological, parasitological, and physiological characteristics of the host. In the period from 2013 to 2021, a total of 770 individuals belonging to the species: Apodemus flavicollis, Apodemus agrarius, Apodemus sylvaticus, Myodes glareolus, Microtus arvalis, and Microtus subterraneus, collected from 42 different localities in Serbia, were examined. Cysts and visible lesions in the body cavity and various organs were detected in a total of 47 individuals. Using the mitochondrial marker 12S rDNA, three species were identified: Taenia taeniaeformis, Taenia martis and Taenia crassiceps. In the entire sample, of all host species, the difference in the percentage of infected individuals depending on the sampling site was proven (χ2=66.7, p=0.007). In addition, diference between rodent species in spleen size, body length and weight, and body condition (BCI) were also significant (Kruskal-Walis test, all p <0.001). In A. flavicollis, males showed higher body length (p=0.024), body mass (p=0.003) and BCI (p=0.003), while in other host species sex did not differ in mentioned parameters (Mann-Whitney test, all p>0.05). In A. flavicollis, spleen and BCI were statistically significantly higher in individuals with tapeworm larvae (p<0.005). Regression analysis in this host showed a positive correlation between spleen size and leukocyte count (WBC) (r=0.221; p=0.014). No difference was found between infected and uninfected individuals for WBC. In M. arvalis, females being more infected than males (χ2=3.884, p=0.049). Regression analysis showed a significant positive correlation of spleen size with body mass (A. agrarius: r=0.669; p<0.001, A. flavicollis: r = 0.668; p<0.001, A. sylvaticus: r = 0.753; p<0.001, M. arvalis: r=0.562; p = 0.005, M. glareolus: r = 0.546; p = 0.004) and BCI (A. agrarius: r=0.608; p <0.001, A. flavicollis: r=0.663; p<0.001, A.sylvaticus: r=0.652; p=0.001, M. arvalis: r=0.481; p=0.020, M. glareolus: r=0.550; p=0.004), except in M. subteraneus. This research represent the preliminary results of molecular analysis of taeniid species in rodents in our country and their relationship with morpho-physiological characteristics of host.
AB  - Многе врсте пантљичара из фамилије Taeniidae од великог су значаја за хуману и ветеринарску медицину, док глодари престављају важне интермедијарне домаћине за неке од врста. Улога глодара у динамици трансмисије пантљичара зависи од различитих спољашњих и унутрашњих фактора. Циљ рада био је утврђивање присуства ларвених облика пантљичара код глодара и молекуларна детекција врсте, као и испитивање односа различитих морфолошких, паразитолошких и физиолошких карактеристика домаћина. У периоду од 2013. до 2021. године прегледано је укупно 770 јединки које припадају врстама: Apodemus flavicollis, Apodemus agrarius, Apodemus sylvaticus, Myodes glareolus, Microtus arvalis, и Microtus subterraneus, прикупљених са 42 различита локалитета на територији Србије. Код укупно 47 јединки детектовано је присуство циста и видљивих лезија у телесној дупљи и различитим органима. Применом митохондријалног маркера 12S rDNA идентификоване су три врсте: Taenia taeniaeformis, Taenia martis i Taenia crassiceps. Посматрајући целокупан узорак свих врста домаћина, доказана је разлика у проценту инфицираних јединки у зависности од локалитета узорковања (χ2 =66.7, p=0.007). Поред тога, разлика између врста глодара у величини слезине, дужини и маси тела и телесног индекса (BCI) била је статистички значајна (Kruskal-Walis тест, сви p<0.001). Код врсте A. flavicollis, мужјаци су имали већу дужину тела (p=0.024), масу (p=0.003) и BCI (p=0.003), док код осталих врста домаћина пол није утицао ни на један од поменутих параметара (Mann-Whitney U тест, сви p>0.05). Код врсте A. flavicollis, слезина и BCI били су статистички значајно већи код јединки са ларвама пантљичара (p<0.005). Регресиона анализа код ове врсте домаћина показала је позитивну корелацију између величине слезине и броја леукоцита (WBC) (r=0.221; p=0.014). Није забележена разлика у броју леукоцита између заражених и незаражених јединки. Код врсте M. arvalis женке су била више инфициране у односу на мужјаке (χ2 =3.884, p=0.049). Регресиона анализа показала је значајну позитивну корелацију тежине слезине са масом тела (A. agrarius: r=0.669; p<0.001, A. flavicollis: r=0.668; p<0.001, A. sylvaticus: r=0.753; p<0.001, M. arvalis: r=0.562; p=0.005, M. glareolus: r=0.546; p=0.004) и BCI (A. agrarius: r=0.608; p<0.001, A. flavicollis: r=0.663; p<0.001, A. sylvaticus: r=0.652; p=0.001, M. arvalis: r=0.481; p=0.020, M. glareolus: r=0.550; p=0.004), изузев код M. subteraneus. Ово истраживање представља прелиминарне резултате молекуларне анализе тенија код глодара у нашој земљи и њиховог односа са морфолошко-физиолошким карактеристикама домаћина.
PB  - Belgrade: Veterinary Association of Serbia
C3  - Book of Abstracts 24th Symposium of epozootiologist and epidemiologist 2022
T1  - Morfološko-fiziološke karakteristike glodara i molekularna detekcija pantljičara
T1  - Morpho-physiological characteristics of rodents and molecular detection of tapeworms
SP  - 142
EP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4992
ER  - 

@conference{
author = "Miljević, Milan and Bajić, Branka and Rajičić, Marija and Budinski, Ivana and Adnađević, Tanja and Blagojević, Jelena",
year = "2022",
abstract = "Many species of tapeworms of the family Taeniidae are of great importance for human and veterinary medicine, while rodents are important intermediate hosts for some of the species. The role of rodents in the tapeworm’s transmission dynamics depends on various external and internal factors. This study aimed to determine the presence of larval forms of tapeworms in rodents and molecular detection of the species, as well as to examine the relationship between different morphological, parasitological, and physiological characteristics of the host. In the period from 2013 to 2021, a total of 770 individuals belonging to the species: Apodemus flavicollis, Apodemus agrarius, Apodemus sylvaticus, Myodes glareolus, Microtus arvalis, and Microtus subterraneus, collected from 42 different localities in Serbia, were examined. Cysts and visible lesions in the body cavity and various organs were detected in a total of 47 individuals. Using the mitochondrial marker 12S rDNA, three species were identified: Taenia taeniaeformis, Taenia martis and Taenia crassiceps. In the entire sample, of all host species, the difference in the percentage of infected individuals depending on the sampling site was proven (χ2=66.7, p=0.007). In addition, diference between rodent species in spleen size, body length and weight, and body condition (BCI) were also significant (Kruskal-Walis test, all p <0.001). In A. flavicollis, males showed higher body length (p=0.024), body mass (p=0.003) and BCI (p=0.003), while in other host species sex did not differ in mentioned parameters (Mann-Whitney test, all p>0.05). In A. flavicollis, spleen and BCI were statistically significantly higher in individuals with tapeworm larvae (p<0.005). Regression analysis in this host showed a positive correlation between spleen size and leukocyte count (WBC) (r=0.221; p=0.014). No difference was found between infected and uninfected individuals for WBC. In M. arvalis, females being more infected than males (χ2=3.884, p=0.049). Regression analysis showed a significant positive correlation of spleen size with body mass (A. agrarius: r=0.669; p<0.001, A. flavicollis: r = 0.668; p<0.001, A. sylvaticus: r = 0.753; p<0.001, M. arvalis: r=0.562; p = 0.005, M. glareolus: r = 0.546; p = 0.004) and BCI (A. agrarius: r=0.608; p <0.001, A. flavicollis: r=0.663; p<0.001, A.sylvaticus: r=0.652; p=0.001, M. arvalis: r=0.481; p=0.020, M. glareolus: r=0.550; p=0.004), except in M. subteraneus. This research represent the preliminary results of molecular analysis of taeniid species in rodents in our country and their relationship with morpho-physiological characteristics of host., Многе врсте пантљичара из фамилије Taeniidae од великог су значаја за хуману и ветеринарску медицину, док глодари престављају важне интермедијарне домаћине за неке од врста. Улога глодара у динамици трансмисије пантљичара зависи од различитих спољашњих и унутрашњих фактора. Циљ рада био је утврђивање присуства ларвених облика пантљичара код глодара и молекуларна детекција врсте, као и испитивање односа различитих морфолошких, паразитолошких и физиолошких карактеристика домаћина. У периоду од 2013. до 2021. године прегледано је укупно 770 јединки које припадају врстама: Apodemus flavicollis, Apodemus agrarius, Apodemus sylvaticus, Myodes glareolus, Microtus arvalis, и Microtus subterraneus, прикупљених са 42 различита локалитета на територији Србије. Код укупно 47 јединки детектовано је присуство циста и видљивих лезија у телесној дупљи и различитим органима. Применом митохондријалног маркера 12S rDNA идентификоване су три врсте: Taenia taeniaeformis, Taenia martis i Taenia crassiceps. Посматрајући целокупан узорак свих врста домаћина, доказана је разлика у проценту инфицираних јединки у зависности од локалитета узорковања (χ2 =66.7, p=0.007). Поред тога, разлика између врста глодара у величини слезине, дужини и маси тела и телесног индекса (BCI) била је статистички значајна (Kruskal-Walis тест, сви p<0.001). Код врсте A. flavicollis, мужјаци су имали већу дужину тела (p=0.024), масу (p=0.003) и BCI (p=0.003), док код осталих врста домаћина пол није утицао ни на један од поменутих параметара (Mann-Whitney U тест, сви p>0.05). Код врсте A. flavicollis, слезина и BCI били су статистички значајно већи код јединки са ларвама пантљичара (p<0.005). Регресиона анализа код ове врсте домаћина показала је позитивну корелацију између величине слезине и броја леукоцита (WBC) (r=0.221; p=0.014). Није забележена разлика у броју леукоцита између заражених и незаражених јединки. Код врсте M. arvalis женке су била више инфициране у односу на мужјаке (χ2 =3.884, p=0.049). Регресиона анализа показала је значајну позитивну корелацију тежине слезине са масом тела (A. agrarius: r=0.669; p<0.001, A. flavicollis: r=0.668; p<0.001, A. sylvaticus: r=0.753; p<0.001, M. arvalis: r=0.562; p=0.005, M. glareolus: r=0.546; p=0.004) и BCI (A. agrarius: r=0.608; p<0.001, A. flavicollis: r=0.663; p<0.001, A. sylvaticus: r=0.652; p=0.001, M. arvalis: r=0.481; p=0.020, M. glareolus: r=0.550; p=0.004), изузев код M. subteraneus. Ово истраживање представља прелиминарне резултате молекуларне анализе тенија код глодара у нашој земљи и њиховог односа са морфолошко-физиолошким карактеристикама домаћина.",
publisher = "Belgrade: Veterinary Association of Serbia",
journal = "Book of Abstracts 24th Symposium of epozootiologist and epidemiologist 2022",
title = "Morfološko-fiziološke karakteristike glodara i molekularna detekcija pantljičara, Morpho-physiological characteristics of rodents and molecular detection of tapeworms",
pages = "142-143",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4992"
}

Miljević, M., Bajić, B., Rajičić, M., Budinski, I., Adnađević, T.,& Blagojević, J.. (2022). Morfološko-fiziološke karakteristike glodara i molekularna detekcija pantljičara. in Book of Abstracts 24th Symposium of epozootiologist and epidemiologist 2022
Belgrade: Veterinary Association of Serbia., 142-143.
https://hdl.handle.net/21.15107/rcub_ibiss_4992

Miljević M, Bajić B, Rajičić M, Budinski I, Adnađević T, Blagojević J. Morfološko-fiziološke karakteristike glodara i molekularna detekcija pantljičara. in Book of Abstracts 24th Symposium of epozootiologist and epidemiologist 2022. 2022;:142-143.
https://hdl.handle.net/21.15107/rcub_ibiss_4992 .

Miljević, Milan, Bajić, Branka, Rajičić, Marija, Budinski, Ivana, Adnađević, Tanja, Blagojević, Jelena, "Morfološko-fiziološke karakteristike glodara i molekularna detekcija pantljičara" in Book of Abstracts 24th Symposium of epozootiologist and epidemiologist 2022 (2022):142-143,
https://hdl.handle.net/21.15107/rcub_ibiss_4992 .

TY  - JOUR
AU  - Rajičić, Marija
AU  - Makunin, Alexey
AU  - Adnađević, Tanja
AU  - Trifonov, Vladimir
AU  - Vujošević, Mladen
AU  - Blagojević, Jelena
PY  - 2022
UR  - https://www.mdpi.com/2075-1729/12/1/50
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4764
AB  - B chromosomes (Bs) are highly polymorphic additional chromosomes in the genomes of many species. Due to the dispensability of Bs and the lack of noticeable phenotypic effects in their carriers, they were considered genetically inert for a long time. Recent studies on Bs in Apodemus flavicollis revealed their genetic composition, potential origin, and spatial organization in the interphase nucleus. Surprisingly, the genetic content of Bs in this species is preserved in all studied samples, even in geographically distinct populations, indicating its biological importance. Using RT-PCR we studied the transcription activity of three genes (Rraga, Haus6, and Cenpe) previously identified on Bs in A. flavicollis. We analysed mRNA isolated from spleen tissues of 34 animals harboring different numbers of Bs (0–3).The products of transcriptional activity of the analysed sequences differ in individuals with and without Bs. We recorded B-genes and/or genes from the standard genome in the presence of Bs, showing sex-dependent higher levels of transcriptional activity. Furthermore, the transcriptional activity of Cenpe varied with the age of the animals differently in the group with and without Bs. With aging, the amount of product was only found to significantly decrease in B carriers. The potential biological significance of all these differences is discussed in the paper.
PB  - Basel: MDPI
T2  - Life
T1  - B Chromosomes’ Sequences in Yellow-Necked Mice Apodemus flavicollis—Exploring the Transcription
IS  - 1
VL  - 12
DO  - 10.3390/life12010050
SP  - 50
ER  - 

@article{
author = "Rajičić, Marija and Makunin, Alexey and Adnađević, Tanja and Trifonov, Vladimir and Vujošević, Mladen and Blagojević, Jelena",
year = "2022",
abstract = "B chromosomes (Bs) are highly polymorphic additional chromosomes in the genomes of many species. Due to the dispensability of Bs and the lack of noticeable phenotypic effects in their carriers, they were considered genetically inert for a long time. Recent studies on Bs in Apodemus flavicollis revealed their genetic composition, potential origin, and spatial organization in the interphase nucleus. Surprisingly, the genetic content of Bs in this species is preserved in all studied samples, even in geographically distinct populations, indicating its biological importance. Using RT-PCR we studied the transcription activity of three genes (Rraga, Haus6, and Cenpe) previously identified on Bs in A. flavicollis. We analysed mRNA isolated from spleen tissues of 34 animals harboring different numbers of Bs (0–3).The products of transcriptional activity of the analysed sequences differ in individuals with and without Bs. We recorded B-genes and/or genes from the standard genome in the presence of Bs, showing sex-dependent higher levels of transcriptional activity. Furthermore, the transcriptional activity of Cenpe varied with the age of the animals differently in the group with and without Bs. With aging, the amount of product was only found to significantly decrease in B carriers. The potential biological significance of all these differences is discussed in the paper.",
publisher = "Basel: MDPI",
journal = "Life",
title = "B Chromosomes’ Sequences in Yellow-Necked Mice Apodemus flavicollis—Exploring the Transcription",
number = "1",
volume = "12",
doi = "10.3390/life12010050",
pages = "50"
}

Rajičić, M., Makunin, A., Adnađević, T., Trifonov, V., Vujošević, M.,& Blagojević, J.. (2022). B Chromosomes’ Sequences in Yellow-Necked Mice Apodemus flavicollis—Exploring the Transcription. in Life
Basel: MDPI., 12(1), 50.
https://doi.org/10.3390/life12010050

Rajičić M, Makunin A, Adnađević T, Trifonov V, Vujošević M, Blagojević J. B Chromosomes’ Sequences in Yellow-Necked Mice Apodemus flavicollis—Exploring the Transcription. in Life. 2022;12(1):50.
doi:10.3390/life12010050 .

Rajičić, Marija, Makunin, Alexey, Adnađević, Tanja, Trifonov, Vladimir, Vujošević, Mladen, Blagojević, Jelena, "B Chromosomes’ Sequences in Yellow-Necked Mice Apodemus flavicollis—Exploring the Transcription" in Life, 12, no. 1 (2022):50,
https://doi.org/10.3390/life12010050 . .

TY  - CONF
AU  - Rončević, Aleksa
AU  - Rajičić, Marija
AU  - Budinski, Ivana
AU  - Miljević, Milan
AU  - Bajić, Branka
AU  - Adnađević, Tanja
AU  - Blagojević, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5191
AB  - The global process of urbanization largely affects the natural environment and its wildlife. The main effects of that process are the fragmentation of habitats and their mutual isolation. Urban infrastructure surrounds preserved forest areas in the cities and prevents the migration of small mammals between them. Yellow-necked mice Apodemus flavicollis is a widespread species in Palearctic forests. Due to extensive urbanization processes in Belgrade city, we wanted to explore its effects on morphometric variation using skulls of this species as convenient objects. We chose two urban and two natural forest populations. One of the chosen locations in Belgrade has been isolated for a long time, while the highway relatively recently separated the other. The main goal of this study was to understand how habitat alteration influence populations of A. flavicollis by determining differences in morphological variation among studied populations. A total of 95 skulls of A. flavicollis adult and subadult animals were analysed from four locations in Serbia, two urban (Košutnjak 29; Stepin gaj 22) and two natural (Avala 30; Senokos 15). Animals were collected from 2015 to 2020, sacrificed, and their skulls were cleaned using dermestid beetles. Images of the mandibles and skulls, together with the scale in millimeters, were obtained with a digital camera. We digitized 14 mandible and 30 cranium landmarks using tpsDig software. The size (Centroid size) and shape (Procrustes distances) variations of the left mandibles and cranium’s ventral side were compared between populations and sexes using landmark-based geometric morphometry in MorphoJ software. Skull and mandible shape variations differed significantly among the central urban population on Košutnjak and the other three analyzed populations and between the population of Stepin gaj and the most distant location from Belgrade, Senokos. The same pattern of variations between analyzed populations was observed for the mandible and the cranium regarding the size. Differences between sexes were not observed in terms of locations or the size of the mandible and cranium. Two natural populations did not morphologically differ from each other. Obtained results indicate that the urban populations of A. flavicollis are morphologically affected by urbanization. The urban environment produces a decrease in migration rate between forest patches and thus decreases gene flow. In theory, small populations, highly isolated, are becoming exposed to the strong effect of genetic drift. The most isolated population from Košutnjak shows limited phenotypic variability, otherwise characteristic of the island population. The other urban population, Stepin gaj differs from the closely located Košutnjak but not from the natural population from Avala, which is also closely located but relatively recently separated by highway and regional roads. Using landmark-based geometric morphometry, we obtained that natural and urban (Belgrade) populations of yellow-necked mouse A. flavicollis are divergent in skull traits. We suggest that phenotypic divergence is associated with human activity that leads to the isolation of natural habitats and their conversion into urban forests.
PB  - Skopje: Macedonian Ecological Society
C3  - Abstract book: 6th Congress of Ecologists of the Republic of North Macedonia, with International Participation; 2022 Oct 15-18; Ohrid, North Macedonia
T1  - The effects of urban habitats on morpholological variations of a skull in populations of yellow-necked mice Apodemus flavicollis
SP  - 165
EP  - 165
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5191
ER  - 

@conference{
author = "Rončević, Aleksa and Rajičić, Marija and Budinski, Ivana and Miljević, Milan and Bajić, Branka and Adnađević, Tanja and Blagojević, Jelena",
year = "2022",
abstract = "The global process of urbanization largely affects the natural environment and its wildlife. The main effects of that process are the fragmentation of habitats and their mutual isolation. Urban infrastructure surrounds preserved forest areas in the cities and prevents the migration of small mammals between them. Yellow-necked mice Apodemus flavicollis is a widespread species in Palearctic forests. Due to extensive urbanization processes in Belgrade city, we wanted to explore its effects on morphometric variation using skulls of this species as convenient objects. We chose two urban and two natural forest populations. One of the chosen locations in Belgrade has been isolated for a long time, while the highway relatively recently separated the other. The main goal of this study was to understand how habitat alteration influence populations of A. flavicollis by determining differences in morphological variation among studied populations. A total of 95 skulls of A. flavicollis adult and subadult animals were analysed from four locations in Serbia, two urban (Košutnjak 29; Stepin gaj 22) and two natural (Avala 30; Senokos 15). Animals were collected from 2015 to 2020, sacrificed, and their skulls were cleaned using dermestid beetles. Images of the mandibles and skulls, together with the scale in millimeters, were obtained with a digital camera. We digitized 14 mandible and 30 cranium landmarks using tpsDig software. The size (Centroid size) and shape (Procrustes distances) variations of the left mandibles and cranium’s ventral side were compared between populations and sexes using landmark-based geometric morphometry in MorphoJ software. Skull and mandible shape variations differed significantly among the central urban population on Košutnjak and the other three analyzed populations and between the population of Stepin gaj and the most distant location from Belgrade, Senokos. The same pattern of variations between analyzed populations was observed for the mandible and the cranium regarding the size. Differences between sexes were not observed in terms of locations or the size of the mandible and cranium. Two natural populations did not morphologically differ from each other. Obtained results indicate that the urban populations of A. flavicollis are morphologically affected by urbanization. The urban environment produces a decrease in migration rate between forest patches and thus decreases gene flow. In theory, small populations, highly isolated, are becoming exposed to the strong effect of genetic drift. The most isolated population from Košutnjak shows limited phenotypic variability, otherwise characteristic of the island population. The other urban population, Stepin gaj differs from the closely located Košutnjak but not from the natural population from Avala, which is also closely located but relatively recently separated by highway and regional roads. Using landmark-based geometric morphometry, we obtained that natural and urban (Belgrade) populations of yellow-necked mouse A. flavicollis are divergent in skull traits. We suggest that phenotypic divergence is associated with human activity that leads to the isolation of natural habitats and their conversion into urban forests.",
publisher = "Skopje: Macedonian Ecological Society",
journal = "Abstract book: 6th Congress of Ecologists of the Republic of North Macedonia, with International Participation; 2022 Oct 15-18; Ohrid, North Macedonia",
title = "The effects of urban habitats on morpholological variations of a skull in populations of yellow-necked mice Apodemus flavicollis",
pages = "165-165",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5191"
}

Rončević, A., Rajičić, M., Budinski, I., Miljević, M., Bajić, B., Adnađević, T.,& Blagojević, J.. (2022). The effects of urban habitats on morpholological variations of a skull in populations of yellow-necked mice Apodemus flavicollis. in Abstract book: 6th Congress of Ecologists of the Republic of North Macedonia, with International Participation; 2022 Oct 15-18; Ohrid, North Macedonia
Skopje: Macedonian Ecological Society., 165-165.
https://hdl.handle.net/21.15107/rcub_ibiss_5191

Rončević A, Rajičić M, Budinski I, Miljević M, Bajić B, Adnađević T, Blagojević J. The effects of urban habitats on morpholological variations of a skull in populations of yellow-necked mice Apodemus flavicollis. in Abstract book: 6th Congress of Ecologists of the Republic of North Macedonia, with International Participation; 2022 Oct 15-18; Ohrid, North Macedonia. 2022;:165-165.
https://hdl.handle.net/21.15107/rcub_ibiss_5191 .

Rončević, Aleksa, Rajičić, Marija, Budinski, Ivana, Miljević, Milan, Bajić, Branka, Adnađević, Tanja, Blagojević, Jelena, "The effects of urban habitats on morpholological variations of a skull in populations of yellow-necked mice Apodemus flavicollis" in Abstract book: 6th Congress of Ecologists of the Republic of North Macedonia, with International Participation; 2022 Oct 15-18; Ohrid, North Macedonia (2022):165-165,
https://hdl.handle.net/21.15107/rcub_ibiss_5191 .

TY  - CONF
AU  - Miljević, Milan
AU  - Čabrilo, Borislav
AU  - Tošić, Božana
AU  - Budinski, Ivana
AU  - Rajičić, Marija
AU  - Bajić, Branka
AU  - Adnađević, Tanja
AU  - Bjelić Čabrilo, Olivera
AU  - Blagojević, Jelena
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4503
AB  - Background. In wild populatons of small rodents, different intrinsic and extrinsic factors affect variation in parasite burdens. The energetc status of animals has a great impact on the immune system, while environmental factors can change the relatonship between infecton and the host body conditon. We aimed to analyse the relatonship between relevant morphological traits and parasitological parameters in populatons of yellow-necked mice, Apodemus flavicollis (Melchior, 1834).
Material and Methods. Nematode burdens were assessed in 49 A. flavicollis, from 7 different localities in Serbia. Morphometric data were measured for all captured mice. Spleen mass was used as a proxy of immunocompetence. The host body conditon, individual parasite load (IndPL), individual parasite species richness (IndPSR), prevalence, mean abundance and mean infecton intensity were calculated.
Results. A total of 12 nematode species were recorded in A. flavicollis. The overall prevalence of infection was very high (100%). Individual parasite species richness (IndPSR) was signifcantly positvely correlated with body conditon (R2=0.173; p=0.003), and the same patern was observed in both sexes. Spleen size was also signifcantly positvely correlated with body conditon (R2=0.344, p<0.005) and body mass (R2=0.341, p<0.005). Contrary to expected, no parasitological indices (IndPSR and IndPL) were signifcantly related to spleen mass.
Conclusion. Animals exhibitng beter body conditons are parasitzed simultaneously with a higher number of nematode species (i.e. their parasite infracommunites are richer). This could prevent any of them to become overabundant in host.
PB  - Belgrade: Serbian Society of Parasitology
C3  - Programme & Abstract book: 13th European Multicolloquium of Parasitology; 2021 Oct 12-16; Belgrade, Serbia
T1  - Parasite load of nematode species in Apodemus flavicollis: Effects of host spleen size, body mass, body condition and sex
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4503
ER  - 

@conference{
author = "Miljević, Milan and Čabrilo, Borislav and Tošić, Božana and Budinski, Ivana and Rajičić, Marija and Bajić, Branka and Adnađević, Tanja and Bjelić Čabrilo, Olivera and Blagojević, Jelena",
year = "2021",
abstract = "Background. In wild populatons of small rodents, different intrinsic and extrinsic factors affect variation in parasite burdens. The energetc status of animals has a great impact on the immune system, while environmental factors can change the relatonship between infecton and the host body conditon. We aimed to analyse the relatonship between relevant morphological traits and parasitological parameters in populatons of yellow-necked mice, Apodemus flavicollis (Melchior, 1834).
Material and Methods. Nematode burdens were assessed in 49 A. flavicollis, from 7 different localities in Serbia. Morphometric data were measured for all captured mice. Spleen mass was used as a proxy of immunocompetence. The host body conditon, individual parasite load (IndPL), individual parasite species richness (IndPSR), prevalence, mean abundance and mean infecton intensity were calculated.
Results. A total of 12 nematode species were recorded in A. flavicollis. The overall prevalence of infection was very high (100%). Individual parasite species richness (IndPSR) was signifcantly positvely correlated with body conditon (R2=0.173; p=0.003), and the same patern was observed in both sexes. Spleen size was also signifcantly positvely correlated with body conditon (R2=0.344, p<0.005) and body mass (R2=0.341, p<0.005). Contrary to expected, no parasitological indices (IndPSR and IndPL) were signifcantly related to spleen mass.
Conclusion. Animals exhibitng beter body conditons are parasitzed simultaneously with a higher number of nematode species (i.e. their parasite infracommunites are richer). This could prevent any of them to become overabundant in host.",
publisher = "Belgrade: Serbian Society of Parasitology",
journal = "Programme & Abstract book: 13th European Multicolloquium of Parasitology; 2021 Oct 12-16; Belgrade, Serbia",
title = "Parasite load of nematode species in Apodemus flavicollis: Effects of host spleen size, body mass, body condition and sex",
pages = "81",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4503"
}

Miljević, M., Čabrilo, B., Tošić, B., Budinski, I., Rajičić, M., Bajić, B., Adnađević, T., Bjelić Čabrilo, O.,& Blagojević, J.. (2021). Parasite load of nematode species in Apodemus flavicollis: Effects of host spleen size, body mass, body condition and sex. in Programme & Abstract book: 13th European Multicolloquium of Parasitology; 2021 Oct 12-16; Belgrade, Serbia
Belgrade: Serbian Society of Parasitology., 81.
https://hdl.handle.net/21.15107/rcub_ibiss_4503

Miljević M, Čabrilo B, Tošić B, Budinski I, Rajičić M, Bajić B, Adnađević T, Bjelić Čabrilo O, Blagojević J. Parasite load of nematode species in Apodemus flavicollis: Effects of host spleen size, body mass, body condition and sex. in Programme & Abstract book: 13th European Multicolloquium of Parasitology; 2021 Oct 12-16; Belgrade, Serbia. 2021;:81.
https://hdl.handle.net/21.15107/rcub_ibiss_4503 .

Miljević, Milan, Čabrilo, Borislav, Tošić, Božana, Budinski, Ivana, Rajičić, Marija, Bajić, Branka, Adnađević, Tanja, Bjelić Čabrilo, Olivera, Blagojević, Jelena, "Parasite load of nematode species in Apodemus flavicollis: Effects of host spleen size, body mass, body condition and sex" in Programme & Abstract book: 13th European Multicolloquium of Parasitology; 2021 Oct 12-16; Belgrade, Serbia (2021):81,
https://hdl.handle.net/21.15107/rcub_ibiss_4503 .

TY  - JOUR
AU  - Blagojević, Jelena
AU  - Šekler, Milanko
AU  - Rajičić, Marija
AU  - Bajić, Branka
AU  - Budinski, Ivana
AU  - Jovanović, Vladimir
AU  - Adnađević, Tanja
AU  - Vidanović, Dejan
AU  - Matović, Kazimir
AU  - Vujošević, Mladen
PY  - 2019
UR  - https://www.akademiai.com/doi/10.1556/004.2019.035
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3479
AB  - The greatest epidemiological significance of leptospirosis in Europe comes from the fact that it is the most widespread zoonosis in the world. However, epizootiological data, especially information on maintenance hosts such as small wild mammals, are largely missing. To fill this gap in data in Serbia, we used RT-PCR for the detection of pathogenic Leptospira species and analysed 107 animals belonging to six species of small wild mammals (Apodemus agrarius, Apodemus flavicollis, Microtus arvalis, Myodes glareolus, Microtus subterraneus and Sorex araneus) collected from two localities. The animals from the first locality that was situated in a tourist area, were collected for four consecutive years (2014-2017). We found persistent incidence of infection from year to year ranging from 6.67% to 78.57%. The average frequency of infected animals was 33.3% with the highest frequency in 2014, the year characterised by a very high number of flooding days. All animals proved to be infected with pathogenic Leptospira species that were collected from the second locality situated in an agricultural area in a single year, 2014. The findings show a variable but constant presence of pathogenic Leptospira species in populations of small wild mammals in the studied areas, which indicates the need for constant monitoring.
T2  - Acta Veterinaria Hungarica
T1  - The prevalence of pathogenic forms of Leptospira in natural populations of small wild mammals in Serbia.
IS  - 3
VL  - 67
DO  - 10.1556/004.2019.035
SP  - 338
EP  - 346
ER  - 

@article{
author = "Blagojević, Jelena and Šekler, Milanko and Rajičić, Marija and Bajić, Branka and Budinski, Ivana and Jovanović, Vladimir and Adnađević, Tanja and Vidanović, Dejan and Matović, Kazimir and Vujošević, Mladen",
year = "2019",
abstract = "The greatest epidemiological significance of leptospirosis in Europe comes from the fact that it is the most widespread zoonosis in the world. However, epizootiological data, especially information on maintenance hosts such as small wild mammals, are largely missing. To fill this gap in data in Serbia, we used RT-PCR for the detection of pathogenic Leptospira species and analysed 107 animals belonging to six species of small wild mammals (Apodemus agrarius, Apodemus flavicollis, Microtus arvalis, Myodes glareolus, Microtus subterraneus and Sorex araneus) collected from two localities. The animals from the first locality that was situated in a tourist area, were collected for four consecutive years (2014-2017). We found persistent incidence of infection from year to year ranging from 6.67% to 78.57%. The average frequency of infected animals was 33.3% with the highest frequency in 2014, the year characterised by a very high number of flooding days. All animals proved to be infected with pathogenic Leptospira species that were collected from the second locality situated in an agricultural area in a single year, 2014. The findings show a variable but constant presence of pathogenic Leptospira species in populations of small wild mammals in the studied areas, which indicates the need for constant monitoring.",
journal = "Acta Veterinaria Hungarica",
title = "The prevalence of pathogenic forms of Leptospira in natural populations of small wild mammals in Serbia.",
number = "3",
volume = "67",
doi = "10.1556/004.2019.035",
pages = "338-346"
}

Blagojević, J., Šekler, M., Rajičić, M., Bajić, B., Budinski, I., Jovanović, V., Adnađević, T., Vidanović, D., Matović, K.,& Vujošević, M.. (2019). The prevalence of pathogenic forms of Leptospira in natural populations of small wild mammals in Serbia.. in Acta Veterinaria Hungarica, 67(3), 338-346.
https://doi.org/10.1556/004.2019.035

Blagojević J, Šekler M, Rajičić M, Bajić B, Budinski I, Jovanović V, Adnađević T, Vidanović D, Matović K, Vujošević M. The prevalence of pathogenic forms of Leptospira in natural populations of small wild mammals in Serbia.. in Acta Veterinaria Hungarica. 2019;67(3):338-346.
doi:10.1556/004.2019.035 .

Blagojević, Jelena, Šekler, Milanko, Rajičić, Marija, Bajić, Branka, Budinski, Ivana, Jovanović, Vladimir, Adnađević, Tanja, Vidanović, Dejan, Matović, Kazimir, Vujošević, Mladen, "The prevalence of pathogenic forms of Leptospira in natural populations of small wild mammals in Serbia." in Acta Veterinaria Hungarica, 67, no. 3 (2019):338-346,
https://doi.org/10.1556/004.2019.035 . .

TY  - JOUR
AU  - Budinski, Ivana
AU  - Blagojević, Jelena
AU  - Jovanović, Vladimir
AU  - Bajić, Branka
AU  - Adnađević, Tanja
AU  - Paunović, Milan
AU  - Vujošević, Mladen
PY  - 2019
UR  - http://dx.plos.org/10.1371/journal.pone.0210321
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6353099
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3262
AB  - Migratory behaviour, sociality and roost selection have a great impact on the population structure of one species. Many bat species live in groups, and movements between summer and hibernation sites are common in temperate bats. The Mediterranean horseshoe bat Rhinolophus euryale is a cave-dwelling species that exhibits roost philopatry and undertakes seasonal movements which are usually shorter than 50 km. Its distribution in Serbia is restricted to karstic areas in western and eastern parts of the country, with a lack of known roosts between them. In this study, microsatellite markers were used to evaluate genetic variation in this species in the Central Balkans. Specifically, spatial genetic structuring between geographic regions and relatedness within different colony types were assessed. All analysed loci were polymorphic, and there was no significant inbreeding coefficient recorded. A moderate degree of genetic differentiation among the sampled colonies was found, and significant isolation by distance was recorded. Our results revealed that populations show a tendency to segregate into three clusters. Unexpectedly, populations from Montenegro and Eastern Serbia tended to group into one cluster, while populations from Western Serbia and Slovenia represented second and third cluster, respectively. The majority of variance was partitioned within colonies, and only a small but significant portion among clusters. Average relatedness within colony members was close to zero, did not differ significantly between the different colony types, and kinship is unlikely to be a major grouping mechanism in this species.
T2  - PloS One
T1  - Population genetic structure of the Mediterranean horseshoe bat Rhinolophus euryale in the central Balkans.
IS  - 1
VL  - 14
DO  - 10.1371/journal.pone.0210321
SP  - e0210321
ER  - 

@article{
author = "Budinski, Ivana and Blagojević, Jelena and Jovanović, Vladimir and Bajić, Branka and Adnađević, Tanja and Paunović, Milan and Vujošević, Mladen",
year = "2019",
abstract = "Migratory behaviour, sociality and roost selection have a great impact on the population structure of one species. Many bat species live in groups, and movements between summer and hibernation sites are common in temperate bats. The Mediterranean horseshoe bat Rhinolophus euryale is a cave-dwelling species that exhibits roost philopatry and undertakes seasonal movements which are usually shorter than 50 km. Its distribution in Serbia is restricted to karstic areas in western and eastern parts of the country, with a lack of known roosts between them. In this study, microsatellite markers were used to evaluate genetic variation in this species in the Central Balkans. Specifically, spatial genetic structuring between geographic regions and relatedness within different colony types were assessed. All analysed loci were polymorphic, and there was no significant inbreeding coefficient recorded. A moderate degree of genetic differentiation among the sampled colonies was found, and significant isolation by distance was recorded. Our results revealed that populations show a tendency to segregate into three clusters. Unexpectedly, populations from Montenegro and Eastern Serbia tended to group into one cluster, while populations from Western Serbia and Slovenia represented second and third cluster, respectively. The majority of variance was partitioned within colonies, and only a small but significant portion among clusters. Average relatedness within colony members was close to zero, did not differ significantly between the different colony types, and kinship is unlikely to be a major grouping mechanism in this species.",
journal = "PloS One",
title = "Population genetic structure of the Mediterranean horseshoe bat Rhinolophus euryale in the central Balkans.",
number = "1",
volume = "14",
doi = "10.1371/journal.pone.0210321",
pages = "e0210321"
}

Budinski, I., Blagojević, J., Jovanović, V., Bajić, B., Adnađević, T., Paunović, M.,& Vujošević, M.. (2019). Population genetic structure of the Mediterranean horseshoe bat Rhinolophus euryale in the central Balkans.. in PloS One, 14(1), e0210321.
https://doi.org/10.1371/journal.pone.0210321

Budinski I, Blagojević J, Jovanović V, Bajić B, Adnađević T, Paunović M, Vujošević M. Population genetic structure of the Mediterranean horseshoe bat Rhinolophus euryale in the central Balkans.. in PloS One. 2019;14(1):e0210321.
doi:10.1371/journal.pone.0210321 .

Budinski, Ivana, Blagojević, Jelena, Jovanović, Vladimir, Bajić, Branka, Adnađević, Tanja, Paunović, Milan, Vujošević, Mladen, "Population genetic structure of the Mediterranean horseshoe bat Rhinolophus euryale in the central Balkans." in PloS One, 14, no. 1 (2019):e0210321,
https://doi.org/10.1371/journal.pone.0210321 . .

TY  - CONF
AU  - Blagojević, Jelena
AU  - Jovanović, Vladimir
AU  - Budinski, Ivana
AU  - Rajičić, Marija
AU  - Adnađević, Tanja
AU  - Vujošević, Mladen
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4850
AB  - Water, being of critical importance for the survival of life on the planet, requires our permanent care and attention. There is a growing concern about the genotoxicity of complex environmental mixtures present in surface waters, due to the risk of genetic damage and cancer, both in aquatic organisms and humans. The present study focuses on exploring the status of water pollution in areas with combined industrial and agricultural activities in order to estimate the magnitude of toxicity and genotoxicity using the Allium test. We collected samples from the Sava and Danube rivers, upstream and downstream of Šabac (Sava River) and Smederevo (Danube River). In both rivers sampling was done in periods of low and high water levels. Levels of toxicity were low in both rivers, although the presence of organic pollution was seen from higher root mean length in all sample groups than in negative controls. The highest values of genotoxicity at locations upstream of both Šabac and Smederevo were obtained in samples collected in a period of high water level, but only the upstream sample from the Sava River had reached a significant level of genotoxicity. This can be explained as a consequence of intensive agricultural activities. Our results indicate that communal town and industrial wastewaters influence river water quality more significantly during low level regimes, while high water levels increase the risk of exposure to chemicals used in agriculture.
PB  - Belgrade: Serbian Genetic Society
C3  - Book of Proceedings 6th Congress of Serbian Genetic Society
T1  - Influence of town wastewaters and agricultural activities on water genotoxicity during different river level regimes
SP  - 7
EP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4850
ER  - 

@conference{
author = "Blagojević, Jelena and Jovanović, Vladimir and Budinski, Ivana and Rajičić, Marija and Adnađević, Tanja and Vujošević, Mladen",
year = "2019",
abstract = "Water, being of critical importance for the survival of life on the planet, requires our permanent care and attention. There is a growing concern about the genotoxicity of complex environmental mixtures present in surface waters, due to the risk of genetic damage and cancer, both in aquatic organisms and humans. The present study focuses on exploring the status of water pollution in areas with combined industrial and agricultural activities in order to estimate the magnitude of toxicity and genotoxicity using the Allium test. We collected samples from the Sava and Danube rivers, upstream and downstream of Šabac (Sava River) and Smederevo (Danube River). In both rivers sampling was done in periods of low and high water levels. Levels of toxicity were low in both rivers, although the presence of organic pollution was seen from higher root mean length in all sample groups than in negative controls. The highest values of genotoxicity at locations upstream of both Šabac and Smederevo were obtained in samples collected in a period of high water level, but only the upstream sample from the Sava River had reached a significant level of genotoxicity. This can be explained as a consequence of intensive agricultural activities. Our results indicate that communal town and industrial wastewaters influence river water quality more significantly during low level regimes, while high water levels increase the risk of exposure to chemicals used in agriculture.",
publisher = "Belgrade: Serbian Genetic Society",
journal = "Book of Proceedings 6th Congress of Serbian Genetic Society",
title = "Influence of town wastewaters and agricultural activities on water genotoxicity during different river level regimes",
pages = "7-14",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4850"
}

Blagojević, J., Jovanović, V., Budinski, I., Rajičić, M., Adnađević, T.,& Vujošević, M.. (2019). Influence of town wastewaters and agricultural activities on water genotoxicity during different river level regimes. in Book of Proceedings 6th Congress of Serbian Genetic Society
Belgrade: Serbian Genetic Society., 7-14.
https://hdl.handle.net/21.15107/rcub_ibiss_4850

Blagojević J, Jovanović V, Budinski I, Rajičić M, Adnađević T, Vujošević M. Influence of town wastewaters and agricultural activities on water genotoxicity during different river level regimes. in Book of Proceedings 6th Congress of Serbian Genetic Society. 2019;:7-14.
https://hdl.handle.net/21.15107/rcub_ibiss_4850 .

Blagojević, Jelena, Jovanović, Vladimir, Budinski, Ivana, Rajičić, Marija, Adnađević, Tanja, Vujošević, Mladen, "Influence of town wastewaters and agricultural activities on water genotoxicity during different river level regimes" in Book of Proceedings 6th Congress of Serbian Genetic Society (2019):7-14,
https://hdl.handle.net/21.15107/rcub_ibiss_4850 .

TY  - CONF
AU  - Blagojević, Jelena
AU  - Jovanović, Vladimir
AU  - Budinski, Ivana
AU  - Rajičić, Marija
AU  - Adnađević, Tanja
AU  - Vujošević, Mladen
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4846
AB  - Water, being of critical importance for the survival of life on the planet, requires our
permanent care and attention. There is a growing concern about the genotoxicity of complex
environmental mixtures present in surface waters, due to the risk of genetic damage and
cancer, both in aquatic organisms and humans. Allium anaphase-telophase test has been
accepted as a promising tool to detect, among other things, toxicity and genotoxicity of river
water. The present study has focused on exploring the status of water pollution in areas
with combined industrial and agricultural activities in order to estimate the magnitude
of toxicity and genotoxicity using the Allium test. We collected samples of surface water
from the Sava River, upstream and downstream from the town of Šabaca nd at the Danube
River upstream and downstream from the town Smederevo. We analyzed using the Allium
anaphase-telophase test. In both rivers sampling was done in periods of low and high water
level. While at low water level genotoxicity was higher at points downstream of Šabaca nd
Smederevo, samples collected in periods of highwater level were more genotoxic upstream
from the towns. This can be explained as a consequence of agricultural activities.
PB  - Belgrade: Serbian Genetic Society
C3  - 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
T1  - Influence of town pollutions on levels of water genotoxicity during different water level regimes
SP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4846
ER  - 

@conference{
author = "Blagojević, Jelena and Jovanović, Vladimir and Budinski, Ivana and Rajičić, Marija and Adnađević, Tanja and Vujošević, Mladen",
year = "2019",
abstract = "Water, being of critical importance for the survival of life on the planet, requires our
permanent care and attention. There is a growing concern about the genotoxicity of complex
environmental mixtures present in surface waters, due to the risk of genetic damage and
cancer, both in aquatic organisms and humans. Allium anaphase-telophase test has been
accepted as a promising tool to detect, among other things, toxicity and genotoxicity of river
water. The present study has focused on exploring the status of water pollution in areas
with combined industrial and agricultural activities in order to estimate the magnitude
of toxicity and genotoxicity using the Allium test. We collected samples of surface water
from the Sava River, upstream and downstream from the town of Šabaca nd at the Danube
River upstream and downstream from the town Smederevo. We analyzed using the Allium
anaphase-telophase test. In both rivers sampling was done in periods of low and high water
level. While at low water level genotoxicity was higher at points downstream of Šabaca nd
Smederevo, samples collected in periods of highwater level were more genotoxic upstream
from the towns. This can be explained as a consequence of agricultural activities.",
publisher = "Belgrade: Serbian Genetic Society",
journal = "6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia",
title = "Influence of town pollutions on levels of water genotoxicity during different water level regimes",
pages = "129",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4846"
}

Blagojević, J., Jovanović, V., Budinski, I., Rajičić, M., Adnađević, T.,& Vujošević, M.. (2019). Influence of town pollutions on levels of water genotoxicity during different water level regimes. in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
Belgrade: Serbian Genetic Society., 129.
https://hdl.handle.net/21.15107/rcub_ibiss_4846

Blagojević J, Jovanović V, Budinski I, Rajičić M, Adnađević T, Vujošević M. Influence of town pollutions on levels of water genotoxicity during different water level regimes. in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia. 2019;:129.
https://hdl.handle.net/21.15107/rcub_ibiss_4846 .

Blagojević, Jelena, Jovanović, Vladimir, Budinski, Ivana, Rajičić, Marija, Adnađević, Tanja, Vujošević, Mladen, "Influence of town pollutions on levels of water genotoxicity during different water level regimes" in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia (2019):129,
https://hdl.handle.net/21.15107/rcub_ibiss_4846 .

TY  - JOUR
AU  - Blagojević, Jelena
AU  - Rajičić, Marija
AU  - Jovanović, Vladimir
AU  - Adnađević, Tanja
AU  - Budinski, Ivana
AU  - Bajić, Branka
AU  - Vujošević, Mladen
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641800004B
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/2364
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3283
AB  - Arvicoline voles are known as a karyotypically extremely polymorphic group in which the genus Microtus leads with the highest rate of karyotype change. A member of this genus, the European pine vole Microtus subterraneus (de Selys-Longchamps, 1836), is widely distributed in Europe and parts of Asia. There are two cytotypes differing in diploid chromosome number, 2n=54 and 52, each showing different chromosomal polymorphisms. At two localities in southeastern Serbia, Mt. Jastrebac and Vlasina, we found the 2n=52 cytotype. Meiotic preparations from males revealed the presence of asynaptic sex chromosomes. Although asynaptic sex chromosomes are frequent in Microtus, this is the first finding for M. subterraneus. From summarized data it appears that two-thirds of the studied species, mainly from Microtus and Terricola subgenera, possess asynaptic sex chromosomes.
T2  - Archives of Biological Sciences
T1  - One more Microtus species with asynaptic sex chromosomes
IS  - 3
VL  - 70
DO  - 10.2298/ABS171113004B
SP  - 443
EP  - 447
ER  - 

@article{
author = "Blagojević, Jelena and Rajičić, Marija and Jovanović, Vladimir and Adnađević, Tanja and Budinski, Ivana and Bajić, Branka and Vujošević, Mladen",
year = "2018",
abstract = "Arvicoline voles are known as a karyotypically extremely polymorphic group in which the genus Microtus leads with the highest rate of karyotype change. A member of this genus, the European pine vole Microtus subterraneus (de Selys-Longchamps, 1836), is widely distributed in Europe and parts of Asia. There are two cytotypes differing in diploid chromosome number, 2n=54 and 52, each showing different chromosomal polymorphisms. At two localities in southeastern Serbia, Mt. Jastrebac and Vlasina, we found the 2n=52 cytotype. Meiotic preparations from males revealed the presence of asynaptic sex chromosomes. Although asynaptic sex chromosomes are frequent in Microtus, this is the first finding for M. subterraneus. From summarized data it appears that two-thirds of the studied species, mainly from Microtus and Terricola subgenera, possess asynaptic sex chromosomes.",
journal = "Archives of Biological Sciences",
title = "One more Microtus species with asynaptic sex chromosomes",
number = "3",
volume = "70",
doi = "10.2298/ABS171113004B",
pages = "443-447"
}

Blagojević, J., Rajičić, M., Jovanović, V., Adnađević, T., Budinski, I., Bajić, B.,& Vujošević, M.. (2018). One more Microtus species with asynaptic sex chromosomes. in Archives of Biological Sciences, 70(3), 443-447.
https://doi.org/10.2298/ABS171113004B

Blagojević J, Rajičić M, Jovanović V, Adnađević T, Budinski I, Bajić B, Vujošević M. One more Microtus species with asynaptic sex chromosomes. in Archives of Biological Sciences. 2018;70(3):443-447.
doi:10.2298/ABS171113004B .

Blagojević, Jelena, Rajičić, Marija, Jovanović, Vladimir, Adnađević, Tanja, Budinski, Ivana, Bajić, Branka, Vujošević, Mladen, "One more Microtus species with asynaptic sex chromosomes" in Archives of Biological Sciences, 70, no. 3 (2018):443-447,
https://doi.org/10.2298/ABS171113004B . .

TY  - JOUR
AU  - Jovanović, Vladimir
AU  - Čabrilo, Borislav
AU  - Budinski, Ivana
AU  - Bjelić-Čabrilo, Olivera
AU  - Adnađević, Tanja
AU  - Blagojević, Jelena
AU  - Vujošević, Mladen
PY  - 2018
UR  - https://www.cambridge.org/core/product/identifier/S0022149X18000548/type/journal_article
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3111
AB  - The yellow-necked mouse, Apodemus flavicollis, can be considered as a model for genetic polymorphism produced by the frequent presence of supernumerary or B chromosomes (Bs). Host genetic background is rarely taken into account in studies of parasite sex ratio. The main aim of this study was to investigate the range of infrapopulation sex ratios for nematode parasites of the yellow-necked mouse and to determine which factors most influence variation in parasite sex ratios. Six nematode species found in the collected yellow-necked mice were analysed. We confirmed the predominant pattern of female-biased sex ratios in vertebrate parasite infrapopulations. The presence of B chromosomes in host genomes played an important role in infrapopulations of Heligmosomoides polygyrus, Syphacia stroma and Trichuris muris, as hosts with B chromosomes carried a higher proportion of males. The relative increase of males in infrapopulations could result from a shift in parasite life history strategy, induced by adaptation to the specific host genotypes (Bs present). In a meta-analysis with previously published data, the sex determination system was demonstrated to play a significant role in nematode sex ratio variation, as well as specific life history patterns, such as the place of egg hatching.
T2  - Journal of Helminthology
T1  - Host B chromosomes as potential sex ratio distorters of intestinal nematode infrapopulations in the yellow-necked mouse (Apodemus flavicollis).
DO  - 10.1017/S0022149X18000548
ER  - 

@article{
author = "Jovanović, Vladimir and Čabrilo, Borislav and Budinski, Ivana and Bjelić-Čabrilo, Olivera and Adnađević, Tanja and Blagojević, Jelena and Vujošević, Mladen",
year = "2018",
abstract = "The yellow-necked mouse, Apodemus flavicollis, can be considered as a model for genetic polymorphism produced by the frequent presence of supernumerary or B chromosomes (Bs). Host genetic background is rarely taken into account in studies of parasite sex ratio. The main aim of this study was to investigate the range of infrapopulation sex ratios for nematode parasites of the yellow-necked mouse and to determine which factors most influence variation in parasite sex ratios. Six nematode species found in the collected yellow-necked mice were analysed. We confirmed the predominant pattern of female-biased sex ratios in vertebrate parasite infrapopulations. The presence of B chromosomes in host genomes played an important role in infrapopulations of Heligmosomoides polygyrus, Syphacia stroma and Trichuris muris, as hosts with B chromosomes carried a higher proportion of males. The relative increase of males in infrapopulations could result from a shift in parasite life history strategy, induced by adaptation to the specific host genotypes (Bs present). In a meta-analysis with previously published data, the sex determination system was demonstrated to play a significant role in nematode sex ratio variation, as well as specific life history patterns, such as the place of egg hatching.",
journal = "Journal of Helminthology",
title = "Host B chromosomes as potential sex ratio distorters of intestinal nematode infrapopulations in the yellow-necked mouse (Apodemus flavicollis).",
doi = "10.1017/S0022149X18000548"
}

Jovanović, V., Čabrilo, B., Budinski, I., Bjelić-Čabrilo, O., Adnađević, T., Blagojević, J.,& Vujošević, M.. (2018). Host B chromosomes as potential sex ratio distorters of intestinal nematode infrapopulations in the yellow-necked mouse (Apodemus flavicollis).. in Journal of Helminthology.
https://doi.org/10.1017/S0022149X18000548

Jovanović V, Čabrilo B, Budinski I, Bjelić-Čabrilo O, Adnađević T, Blagojević J, Vujošević M. Host B chromosomes as potential sex ratio distorters of intestinal nematode infrapopulations in the yellow-necked mouse (Apodemus flavicollis).. in Journal of Helminthology. 2018;.
doi:10.1017/S0022149X18000548 .

Jovanović, Vladimir, Čabrilo, Borislav, Budinski, Ivana, Bjelić-Čabrilo, Olivera, Adnađević, Tanja, Blagojević, Jelena, Vujošević, Mladen, "Host B chromosomes as potential sex ratio distorters of intestinal nematode infrapopulations in the yellow-necked mouse (Apodemus flavicollis)." in Journal of Helminthology (2018),
https://doi.org/10.1017/S0022149X18000548 . .

TY  - CONF
AU  - Bajić, Branka
AU  - Rajičić, Marija
AU  - Jovanović, Vladimir
AU  - Budinski, Ivana
AU  - Adnađević, Tanja
AU  - Vujošević, Mladen
AU  - Blagojević, Jelena
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4272
AB  - Although polymorphism of the Y chromosome in bank vole, Myodes glareoulus, is a phenomenon
known for more than 40 years, few studies just pointing to the presence of different types of Y
chromosome, have been done since. Total of 113 males have been caught by live traps at 8 localities
from Serbia. Chromosome preparations were done from bone marrow and karyotypes were analyzed
for the presence of different types of Y chromosome. Two types of Y, metacentric and acrocentric, were
detected. Metacentric Y was prevalent (62.83%). Additionally, differential cytogenetic staining was
done to obtain further information about differences between noticed types of Y. In order to check is
there any dependence of variation in frequencies of metacentric Y and geographic parameters (latitude
and longitude) multiple regression analysis was done. Coeffcient of regression was signifcant (R2 =
0.589; F
(2,5)=6.021, p<0.046) but only latitude showed negative signifcant correlation with frequency
of metacentric Y. Its frequency is increasing from North to South in a range from 0.11 to 1.0.
PB  - Skopje: Macedonian Ecological Society
C3  - 5th Congress of ecologists of the Republic of Macedonia with international participation; 2016 Oct 19-22; Ohrid Republic of Macedonia
T1  - Spatial variability and morphology of Y chromosome in bank vole, Myodes glareolus (Mammalia, Rodentia)
SP  - 23
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4272
ER  - 

@conference{
author = "Bajić, Branka and Rajičić, Marija and Jovanović, Vladimir and Budinski, Ivana and Adnađević, Tanja and Vujošević, Mladen and Blagojević, Jelena",
year = "2016",
abstract = "Although polymorphism of the Y chromosome in bank vole, Myodes glareoulus, is a phenomenon
known for more than 40 years, few studies just pointing to the presence of different types of Y
chromosome, have been done since. Total of 113 males have been caught by live traps at 8 localities
from Serbia. Chromosome preparations were done from bone marrow and karyotypes were analyzed
for the presence of different types of Y chromosome. Two types of Y, metacentric and acrocentric, were
detected. Metacentric Y was prevalent (62.83%). Additionally, differential cytogenetic staining was
done to obtain further information about differences between noticed types of Y. In order to check is
there any dependence of variation in frequencies of metacentric Y and geographic parameters (latitude
and longitude) multiple regression analysis was done. Coeffcient of regression was signifcant (R2 =
0.589; F
(2,5)=6.021, p<0.046) but only latitude showed negative signifcant correlation with frequency
of metacentric Y. Its frequency is increasing from North to South in a range from 0.11 to 1.0.",
publisher = "Skopje: Macedonian Ecological Society",
journal = "5th Congress of ecologists of the Republic of Macedonia with international participation; 2016 Oct 19-22; Ohrid Republic of Macedonia",
title = "Spatial variability and morphology of Y chromosome in bank vole, Myodes glareolus (Mammalia, Rodentia)",
pages = "23",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4272"
}

Bajić, B., Rajičić, M., Jovanović, V., Budinski, I., Adnađević, T., Vujošević, M.,& Blagojević, J.. (2016). Spatial variability and morphology of Y chromosome in bank vole, Myodes glareolus (Mammalia, Rodentia). in 5th Congress of ecologists of the Republic of Macedonia with international participation; 2016 Oct 19-22; Ohrid Republic of Macedonia
Skopje: Macedonian Ecological Society., 23.
https://hdl.handle.net/21.15107/rcub_ibiss_4272

Bajić B, Rajičić M, Jovanović V, Budinski I, Adnađević T, Vujošević M, Blagojević J. Spatial variability and morphology of Y chromosome in bank vole, Myodes glareolus (Mammalia, Rodentia). in 5th Congress of ecologists of the Republic of Macedonia with international participation; 2016 Oct 19-22; Ohrid Republic of Macedonia. 2016;:23.
https://hdl.handle.net/21.15107/rcub_ibiss_4272 .

Bajić, Branka, Rajičić, Marija, Jovanović, Vladimir, Budinski, Ivana, Adnađević, Tanja, Vujošević, Mladen, Blagojević, Jelena, "Spatial variability and morphology of Y chromosome in bank vole, Myodes glareolus (Mammalia, Rodentia)" in 5th Congress of ecologists of the Republic of Macedonia with international participation; 2016 Oct 19-22; Ohrid Republic of Macedonia (2016):23,
https://hdl.handle.net/21.15107/rcub_ibiss_4272 .

TY  - CONF
AU  - Cvijanović, Gorčin
AU  - Adnađević, Tanja
AU  - Jarić, Ivan
AU  - Jojić, Vida
AU  - Marić, Saša
AU  - Lenhardt, Mirjana
PY  - 2016
UR  - https://www.fitfish.eu/upload_mm/e/8/5/c5305e7e-0f23-4833-a068-4b4fa8e5c505_FITFISH%20annual%20conference%202016.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5424
AB  - Despite sterlet (Acipenser ruthenus L.) being a less important resource regarding caviar
production, their populations have experienced a decline during the 20th century
throughout its range, mainly due to poorly regulated fishery, pollution, habitat
fragmentation and habitat loss. Stocking programs are implemented throughout Danube
River basin, with Upper Danube populations being dependent on continuous stocking
efforts, while commercial exploitation of wild stocks in the Middle and Lower Danube
has to be compensated with stocking of larvae, fingerlings and juveniles. Selection of
proper specimens for stocking programs should be carefully conducted, since it can lead
to deleterious impact, such as reduction of effective population size, inbreeding and
outbreeding depression, and loss of locally adapted alleles. Therefore, natural
populations should be examined genetically both before and after release of hatcheryreared
juveniles. Our research on Middle and Lower Danube sterlet, suggests that
genetic variability should be attributed almost entirely to individual variability, with a
weak population structure and no clear evidence of a bottleneck and inbreeding within
populations. Also, specimens used for the supportive stocking in the Tisza River in
Hungary originated from the Danube River, so the information about gene flow between
these rivers should be carefully considered. Additionally, most of breeding programs are
focused on genetic diversity and do not acknowledge complexities of wild populations
fitness architecture. Although Middle and Lower Danube dams are recent, in regard to
sterlet population time, they create more lentic conditions that do not suit sterlet
rheophilous nature. Both our previous and current morphometric research suggests that
hatchery-reared sterlet specimens are not necessarily suited for stocking of certain
Danube River sections. Lower Danube section have a different water flow regime and
suspended sediment discharge than those in Middle Danube and in Lower Tisza River,
so the sterlet specimens are differently adapted to their environment, which is in
concordance with our findings. We suggest that both shape analysis and genetic analysis
should be applied in restocking programs.
PB  - Belgrade: Institute for Multidisciplinary Research, University of Belgrade
C3  - Fitfish Annual Conference; 2016 Apr 22; Belgrade, Serbia
T1  - Danube sterlet morphometrics and genetic – guidelines for restocking programs
SP  - 50
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5424
ER  - 

@conference{
author = "Cvijanović, Gorčin and Adnađević, Tanja and Jarić, Ivan and Jojić, Vida and Marić, Saša and Lenhardt, Mirjana",
year = "2016",
abstract = "Despite sterlet (Acipenser ruthenus L.) being a less important resource regarding caviar
production, their populations have experienced a decline during the 20th century
throughout its range, mainly due to poorly regulated fishery, pollution, habitat
fragmentation and habitat loss. Stocking programs are implemented throughout Danube
River basin, with Upper Danube populations being dependent on continuous stocking
efforts, while commercial exploitation of wild stocks in the Middle and Lower Danube
has to be compensated with stocking of larvae, fingerlings and juveniles. Selection of
proper specimens for stocking programs should be carefully conducted, since it can lead
to deleterious impact, such as reduction of effective population size, inbreeding and
outbreeding depression, and loss of locally adapted alleles. Therefore, natural
populations should be examined genetically both before and after release of hatcheryreared
juveniles. Our research on Middle and Lower Danube sterlet, suggests that
genetic variability should be attributed almost entirely to individual variability, with a
weak population structure and no clear evidence of a bottleneck and inbreeding within
populations. Also, specimens used for the supportive stocking in the Tisza River in
Hungary originated from the Danube River, so the information about gene flow between
these rivers should be carefully considered. Additionally, most of breeding programs are
focused on genetic diversity and do not acknowledge complexities of wild populations
fitness architecture. Although Middle and Lower Danube dams are recent, in regard to
sterlet population time, they create more lentic conditions that do not suit sterlet
rheophilous nature. Both our previous and current morphometric research suggests that
hatchery-reared sterlet specimens are not necessarily suited for stocking of certain
Danube River sections. Lower Danube section have a different water flow regime and
suspended sediment discharge than those in Middle Danube and in Lower Tisza River,
so the sterlet specimens are differently adapted to their environment, which is in
concordance with our findings. We suggest that both shape analysis and genetic analysis
should be applied in restocking programs.",
publisher = "Belgrade: Institute for Multidisciplinary Research, University of Belgrade",
journal = "Fitfish Annual Conference; 2016 Apr 22; Belgrade, Serbia",
title = "Danube sterlet morphometrics and genetic – guidelines for restocking programs",
pages = "50",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5424"
}

Cvijanović, G., Adnađević, T., Jarić, I., Jojić, V., Marić, S.,& Lenhardt, M.. (2016). Danube sterlet morphometrics and genetic – guidelines for restocking programs. in Fitfish Annual Conference; 2016 Apr 22; Belgrade, Serbia
Belgrade: Institute for Multidisciplinary Research, University of Belgrade., 50.
https://hdl.handle.net/21.15107/rcub_ibiss_5424

Cvijanović G, Adnađević T, Jarić I, Jojić V, Marić S, Lenhardt M. Danube sterlet morphometrics and genetic – guidelines for restocking programs. in Fitfish Annual Conference; 2016 Apr 22; Belgrade, Serbia. 2016;:50.
https://hdl.handle.net/21.15107/rcub_ibiss_5424 .

Cvijanović, Gorčin, Adnađević, Tanja, Jarić, Ivan, Jojić, Vida, Marić, Saša, Lenhardt, Mirjana, "Danube sterlet morphometrics and genetic – guidelines for restocking programs" in Fitfish Annual Conference; 2016 Apr 22; Belgrade, Serbia (2016):50,
https://hdl.handle.net/21.15107/rcub_ibiss_5424 .

TY  - CONF
AU  - Rajičić, Marija
AU  - Adnađević, Tanja
AU  - Bugarski-Stanojević, Vanja
AU  - Stamenković, Gorana
AU  - Jojić, Vida
AU  - Budinski, Ivana
AU  - Jovanović, Vladimir
AU  - Blagojević, Jelena
AU  - Vujošević, Mladen
PY  - 2015
UR  - http://chromosome2015.mcb.nsc.ru/chr_2015_abstracts.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5423
AB  - In genus Apodemus, the presence of B chromosome has been confirmed in six out of
twenty-two species. One of them is yellow-necked field mouse Apodemus flavicollis. The
presence of B chromosomes has been confirmed in almost all studied populations of A. flavicollis
46
in Serbia in a wide range of frequencies (0,07- 0,63). Five B chromosomes per animal was the
highest number of Bs recorded in our analyzed populations.
These extra chromosomes in A. flavicollis are euchromatic and acrocentric, and by size
and distribution of G- and C-bands can be sorted into the group of five smallest chromosomes in
karyotype.
The absence of Bs accumulation in male meiosis, no significant difference in the mean
number of scars and embryos between females with and without Bs, as well as the absence of
significant difference in the presence of Bs among six age categories, speaks in favor of heterotic
model of their maintenance, contrary to the model of parasitic behavior of these additional
genomic elements.
In general, the number of Bs carriers is higher in environment that is not optimal for this
species which indicates that those individuals have higher fitness. Seasonal variation of Bs
frequencies has been recorded, in such a manner that highest frequencies are present before and
after winter, with stable frequency fluctuations during successive years. Different frequency of Bs
carriers is related to adaptive differentiation to diverse habitats mediated by environmental
variables that directly and/or indirectly influence population dynamics of A. flavicollis. Changes
in frequency follow up increase in population density in circumstances without food and space
competition. Positive correlation between the frequency of Bs carriers and the average number of
sub zero days, as well as with altitude increase, has been recorded.
Considering phenotypic effects, higher level of morphological integration of mandible in
animals with Bs in A. flavicollis has been found. Furthermore, studies of the effects of Bs on
nonmetric cranial traits, as well as on three components of cranial variability confirmed that Bs
does not disturb developmental homeostasis in their carriers. Moreover, Bs play a significant role
in structuring cranial variation.
Negative correlation between the expression of Tgf-β gene and the presence of Bs was also
observed. This could be of great importance for B chromosome which has to pass through
different mitotic and meiotic check points, and Tgf-β gene, through cell cycle regulation,
influences development and homeostasis.
The existence of specific DNA profiles and differential expressions of three genes in the
presence of Bs, testify about interaction of Bs with the rest of the genome. Absence of C-KIT
exon 1 in more than one copy in specimens with 1, 2 and 3B chromosomes excludes the
possibility that this gene is present on Bs. On the other hand, presence of S18 gene copy has been
indicated on some Bs.
Considering the results of population studies, euchromatic structure of Bs and molecular
studies that have been conducted so far, we can say that the contribution of Bs to overall genetic
47
diversity in this species is in sight, but there is still need for more specific evidence such as
precise determination of Bs molecular structure, origin and function.
PB  - Institute of molecular and cellular biology SB RAS
C3  - Abstracts of the International conference “Chromosome 2015”; 2015 Aug 24-28; Novosibirsk, Russia
T1  - B chromosomes in Apodemus flavicollis
SP  - 45
EP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5423
ER  - 

@conference{
author = "Rajičić, Marija and Adnađević, Tanja and Bugarski-Stanojević, Vanja and Stamenković, Gorana and Jojić, Vida and Budinski, Ivana and Jovanović, Vladimir and Blagojević, Jelena and Vujošević, Mladen",
year = "2015",
abstract = "In genus Apodemus, the presence of B chromosome has been confirmed in six out of
twenty-two species. One of them is yellow-necked field mouse Apodemus flavicollis. The
presence of B chromosomes has been confirmed in almost all studied populations of A. flavicollis
46
in Serbia in a wide range of frequencies (0,07- 0,63). Five B chromosomes per animal was the
highest number of Bs recorded in our analyzed populations.
These extra chromosomes in A. flavicollis are euchromatic and acrocentric, and by size
and distribution of G- and C-bands can be sorted into the group of five smallest chromosomes in
karyotype.
The absence of Bs accumulation in male meiosis, no significant difference in the mean
number of scars and embryos between females with and without Bs, as well as the absence of
significant difference in the presence of Bs among six age categories, speaks in favor of heterotic
model of their maintenance, contrary to the model of parasitic behavior of these additional
genomic elements.
In general, the number of Bs carriers is higher in environment that is not optimal for this
species which indicates that those individuals have higher fitness. Seasonal variation of Bs
frequencies has been recorded, in such a manner that highest frequencies are present before and
after winter, with stable frequency fluctuations during successive years. Different frequency of Bs
carriers is related to adaptive differentiation to diverse habitats mediated by environmental
variables that directly and/or indirectly influence population dynamics of A. flavicollis. Changes
in frequency follow up increase in population density in circumstances without food and space
competition. Positive correlation between the frequency of Bs carriers and the average number of
sub zero days, as well as with altitude increase, has been recorded.
Considering phenotypic effects, higher level of morphological integration of mandible in
animals with Bs in A. flavicollis has been found. Furthermore, studies of the effects of Bs on
nonmetric cranial traits, as well as on three components of cranial variability confirmed that Bs
does not disturb developmental homeostasis in their carriers. Moreover, Bs play a significant role
in structuring cranial variation.
Negative correlation between the expression of Tgf-β gene and the presence of Bs was also
observed. This could be of great importance for B chromosome which has to pass through
different mitotic and meiotic check points, and Tgf-β gene, through cell cycle regulation,
influences development and homeostasis.
The existence of specific DNA profiles and differential expressions of three genes in the
presence of Bs, testify about interaction of Bs with the rest of the genome. Absence of C-KIT
exon 1 in more than one copy in specimens with 1, 2 and 3B chromosomes excludes the
possibility that this gene is present on Bs. On the other hand, presence of S18 gene copy has been
indicated on some Bs.
Considering the results of population studies, euchromatic structure of Bs and molecular
studies that have been conducted so far, we can say that the contribution of Bs to overall genetic
47
diversity in this species is in sight, but there is still need for more specific evidence such as
precise determination of Bs molecular structure, origin and function.",
publisher = "Institute of molecular and cellular biology SB RAS",
journal = "Abstracts of the International conference “Chromosome 2015”; 2015 Aug 24-28; Novosibirsk, Russia",
title = "B chromosomes in Apodemus flavicollis",
pages = "45-47",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5423"
}

Rajičić, M., Adnađević, T., Bugarski-Stanojević, V., Stamenković, G., Jojić, V., Budinski, I., Jovanović, V., Blagojević, J.,& Vujošević, M.. (2015). B chromosomes in Apodemus flavicollis. in Abstracts of the International conference “Chromosome 2015”; 2015 Aug 24-28; Novosibirsk, Russia
Institute of molecular and cellular biology SB RAS., 45-47.
https://hdl.handle.net/21.15107/rcub_ibiss_5423

Rajičić M, Adnađević T, Bugarski-Stanojević V, Stamenković G, Jojić V, Budinski I, Jovanović V, Blagojević J, Vujošević M. B chromosomes in Apodemus flavicollis. in Abstracts of the International conference “Chromosome 2015”; 2015 Aug 24-28; Novosibirsk, Russia. 2015;:45-47.
https://hdl.handle.net/21.15107/rcub_ibiss_5423 .

Rajičić, Marija, Adnađević, Tanja, Bugarski-Stanojević, Vanja, Stamenković, Gorana, Jojić, Vida, Budinski, Ivana, Jovanović, Vladimir, Blagojević, Jelena, Vujošević, Mladen, "B chromosomes in Apodemus flavicollis" in Abstracts of the International conference “Chromosome 2015”; 2015 Aug 24-28; Novosibirsk, Russia (2015):45-47,
https://hdl.handle.net/21.15107/rcub_ibiss_5423 .

TY  - JOUR
AU  - Stamenković, Gorana
AU  - Nikolic, V.
AU  - Blagojević, Jelena
AU  - Bugarski-Stanojević, Vanja
AU  - Adnađević, Tanja
AU  - Stanojevic, M.
AU  - Vujošević, Mladen
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1999
AB  - Dobrava-Belgrade virus (DOBV) is a hantavirus species that causes the
   most severe form of haemorrhagic fever with renal syndrome (HFRS) in
   Europe. DOBV has been detected in three Apodemus rodents: A.flavicollis,
   A.agrarius and A.ponticus. These emerging viruses appear throughout the
   Balkan Peninsula including Serbia as its central part. In this study, we
   examined the seroprevalence, molecular epidemiology and phylogenetics of
   DOBV from A.flavicollis captured at six Serbian localities. Furthermore,
   we applied microsatellite typing of host animal genome to analyse the
   role of host kinship in DOBV animal transmission. The overall IgG
   seropositivity rate over 3years (2008-2010) was 11.9\% (22/185). All
   seropositive samples were subjected to RT-PCR and DNA sequencing for S
   and L genome segments (pos. 291-1079nt and 2999-3316nt, respectively).
   DOBV was genetically detected in three samples from mountain Tara in
   western Serbia, a newly detected DOBV focus in the Balkans. No sequence
   data from human cases from Serbia are available for the studied period.
   However, collected DOBV isolates in this work phylogenetically clustered
   together with isolates from Serbian human cases dating from 2002, with
   1.9\% nucleotide divergence. We determined the level of kinship between
   seropositive and seronegative animal groups and found no significant
   difference, suggesting that horizontal virus transmission in the studied
   population was the same within and among the hatches. Our findings are
   the first genetic detection of DOBV in rodents in Serbia. We confirm
   wide and continuous hantavirus presence in the examined parts of the
   Balkans, underlying the necessity of continual monitoring of hantavirus
   circulation in A.flavicollis.
T2  - Zoonoses and Public Health
T1  - Genetic Analysis of Dobrava-Belgrade Virus from Western Serbia - A Newly
 Detected Focus in the Balkan Peninsula
IS  - 2
VL  - 62
DO  - 10.1111/zph.12136
SP  - 141
EP  - 150
ER  - 

@article{
author = "Stamenković, Gorana and Nikolic, V. and Blagojević, Jelena and Bugarski-Stanojević, Vanja and Adnađević, Tanja and Stanojevic, M. and Vujošević, Mladen",
year = "2015",
abstract = "Dobrava-Belgrade virus (DOBV) is a hantavirus species that causes the
   most severe form of haemorrhagic fever with renal syndrome (HFRS) in
   Europe. DOBV has been detected in three Apodemus rodents: A.flavicollis,
   A.agrarius and A.ponticus. These emerging viruses appear throughout the
   Balkan Peninsula including Serbia as its central part. In this study, we
   examined the seroprevalence, molecular epidemiology and phylogenetics of
   DOBV from A.flavicollis captured at six Serbian localities. Furthermore,
   we applied microsatellite typing of host animal genome to analyse the
   role of host kinship in DOBV animal transmission. The overall IgG
   seropositivity rate over 3years (2008-2010) was 11.9\% (22/185). All
   seropositive samples were subjected to RT-PCR and DNA sequencing for S
   and L genome segments (pos. 291-1079nt and 2999-3316nt, respectively).
   DOBV was genetically detected in three samples from mountain Tara in
   western Serbia, a newly detected DOBV focus in the Balkans. No sequence
   data from human cases from Serbia are available for the studied period.
   However, collected DOBV isolates in this work phylogenetically clustered
   together with isolates from Serbian human cases dating from 2002, with
   1.9\% nucleotide divergence. We determined the level of kinship between
   seropositive and seronegative animal groups and found no significant
   difference, suggesting that horizontal virus transmission in the studied
   population was the same within and among the hatches. Our findings are
   the first genetic detection of DOBV in rodents in Serbia. We confirm
   wide and continuous hantavirus presence in the examined parts of the
   Balkans, underlying the necessity of continual monitoring of hantavirus
   circulation in A.flavicollis.",
journal = "Zoonoses and Public Health",
title = "Genetic Analysis of Dobrava-Belgrade Virus from Western Serbia - A Newly
 Detected Focus in the Balkan Peninsula",
number = "2",
volume = "62",
doi = "10.1111/zph.12136",
pages = "141-150"
}

Stamenković, G., Nikolic, V., Blagojević, J., Bugarski-Stanojević, V., Adnađević, T., Stanojevic, M.,& Vujošević, M.. (2015). Genetic Analysis of Dobrava-Belgrade Virus from Western Serbia - A Newly
 Detected Focus in the Balkan Peninsula. in Zoonoses and Public Health, 62(2), 141-150.
https://doi.org/10.1111/zph.12136

Stamenković G, Nikolic V, Blagojević J, Bugarski-Stanojević V, Adnađević T, Stanojevic M, Vujošević M. Genetic Analysis of Dobrava-Belgrade Virus from Western Serbia - A Newly
 Detected Focus in the Balkan Peninsula. in Zoonoses and Public Health. 2015;62(2):141-150.
doi:10.1111/zph.12136 .

Stamenković, Gorana, Nikolic, V., Blagojević, Jelena, Bugarski-Stanojević, Vanja, Adnađević, Tanja, Stanojevic, M., Vujošević, Mladen, "Genetic Analysis of Dobrava-Belgrade Virus from Western Serbia - A Newly
 Detected Focus in the Balkan Peninsula" in Zoonoses and Public Health, 62, no. 2 (2015):141-150,
https://doi.org/10.1111/zph.12136 . .

TY  - JOUR
AU  - Cvijanovic, Gorcin
AU  - Adnađević, Tanja
AU  - Lenhardt, Mirjana
AU  - Maric, Sasa
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2026
AB  - Poor regulated fishery, pollution, fragmentation and loss of habitat are
   most important factors influencing decline of sterlet population
   worldwide. In Middle and Lower Danube region, this species still have
   significant economic importance since wilde populations are commercially
   exploited, while Upper Danube populations are dependent on stocking
   efforts in order to maintain their presence in open waters. Aim of
   present study is to analyze genetic diversity of sterlet populations
   from the Middle and Lower Danube and Lower Tisza rivers, as a
   prerequisite for their effective conservation and management. Analysis
   of a highly variable D-loop fragment of mitochondrial DNA detected five
   new haplotypes, while the eight previously identified haplotypes had
   extended their previous range. Genetic variability could be attributed
   almost entirely to individuals, with observed lack of population
   structure. Negative values of neutrality test indicate recent expansion
   on some sampling locations. Adittionaly, gene flow analysis between
   Lower and Middle Danube region showed intensive exchange of speciemens.
   At the same time analysis showed some influence of Tisza dam on gene
   flow between samples from Tisza and Middle Danube section. Our study
   indicated the need for a careful planning of sterlet stocking programmes
   and inclusion of demographic data or catch time-series.
T2  - Genetika-Belgrade
T1  - New data on sterlet (Acipenser ruthenus L.) genetic diversity in the middle and lower Danube sections, based on mitochondrial DNA analyses
IS  - 3
VL  - 47
DO  - 10.2298/GENSR1503051C
SP  - 1051
EP  - 1062
ER  - 

@article{
author = "Cvijanovic, Gorcin and Adnađević, Tanja and Lenhardt, Mirjana and Maric, Sasa",
year = "2015",
abstract = "Poor regulated fishery, pollution, fragmentation and loss of habitat are
   most important factors influencing decline of sterlet population
   worldwide. In Middle and Lower Danube region, this species still have
   significant economic importance since wilde populations are commercially
   exploited, while Upper Danube populations are dependent on stocking
   efforts in order to maintain their presence in open waters. Aim of
   present study is to analyze genetic diversity of sterlet populations
   from the Middle and Lower Danube and Lower Tisza rivers, as a
   prerequisite for their effective conservation and management. Analysis
   of a highly variable D-loop fragment of mitochondrial DNA detected five
   new haplotypes, while the eight previously identified haplotypes had
   extended their previous range. Genetic variability could be attributed
   almost entirely to individuals, with observed lack of population
   structure. Negative values of neutrality test indicate recent expansion
   on some sampling locations. Adittionaly, gene flow analysis between
   Lower and Middle Danube region showed intensive exchange of speciemens.
   At the same time analysis showed some influence of Tisza dam on gene
   flow between samples from Tisza and Middle Danube section. Our study
   indicated the need for a careful planning of sterlet stocking programmes
   and inclusion of demographic data or catch time-series.",
journal = "Genetika-Belgrade",
title = "New data on sterlet (Acipenser ruthenus L.) genetic diversity in the middle and lower Danube sections, based on mitochondrial DNA analyses",
number = "3",
volume = "47",
doi = "10.2298/GENSR1503051C",
pages = "1051-1062"
}

Cvijanovic, G., Adnađević, T., Lenhardt, M.,& Maric, S.. (2015). New data on sterlet (Acipenser ruthenus L.) genetic diversity in the middle and lower Danube sections, based on mitochondrial DNA analyses. in Genetika-Belgrade, 47(3), 1051-1062.
https://doi.org/10.2298/GENSR1503051C

Cvijanovic G, Adnađević T, Lenhardt M, Maric S. New data on sterlet (Acipenser ruthenus L.) genetic diversity in the middle and lower Danube sections, based on mitochondrial DNA analyses. in Genetika-Belgrade. 2015;47(3):1051-1062.
doi:10.2298/GENSR1503051C .

Cvijanovic, Gorcin, Adnađević, Tanja, Lenhardt, Mirjana, Maric, Sasa, "New data on sterlet (Acipenser ruthenus L.) genetic diversity in the middle and lower Danube sections, based on mitochondrial DNA analyses" in Genetika-Belgrade, 47, no. 3 (2015):1051-1062,
https://doi.org/10.2298/GENSR1503051C . .

TY  - JOUR
AU  - Rajičić, Marija
AU  - Adnađević, Tanja
AU  - Stamenković, Gorana
AU  - Blagojević, Jelena
AU  - Vujošević, Mladen
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2065
AB  - B chromosomes (Bs) are a very heterogeneous group of extra chromosomes.
   In various species Bs occur with different nucleotide sequences ranging
   from repetitive to protein coding. In yellow-necked field mice, Apodemus
   flavicollis Bs are small euchromatic chromosomes and untill now, only
   few molecular analyses have been conducted. In this study we examined A.
   flavicollis individuals with different number of Bs for presence of two
   genes, C-KIT and 18S rRNA. The C-KIT proto-oncogene was found on Bs in
   three Canidae species and one Cervidae species. This gene is a coding
   receptor critical for proliferation and cell differentiation of
   hematopoietic, melanoblast and primordial germ cells, and is highly
   conserved within mammals. While using semiquantitative PCR, we did not
   notice any difference in the C-KIT band intensity among animals with
   different number of Bs (0-3). The presence of only one copy of C-KIT
   gene was confirmed using real time-PCR on genomic DNA of A. flavicollis
   specimens with different number of Bs. rRNA genes in eukaryotes' genome
   are organized like units of tandem repeated sequences. The units form
   distinct clusters on one to several chromosome pairs. rRNA genes were
   found on Bs in different species including two species of genus
   Apodemus. One particular sample with 2 Bs showed the number of 18S rRNA
   gene about three times that of the calibrator 0 B sample. This result
   can indicate the presence of 18S rRNA gene on Bs, but its confirmation
   requires the implementation of other methods. Still, we can neither
   confirm nor deny the existence of pseudogen of tested target genes, or
   lose of exon 1 of C-KIT protooncogen in Bs of A. flavicollis. Our
   findings are further discussed.
T2  - Genetika-Belgrade
T1  - Screening of B chromosomes for presence of two genes in yellow-necked mice, Apodemus flavicollis (Mammalia, Rodentia)
IS  - 1
VL  - 47
DO  - 10.2298/GENSR1501311R
SP  - 311
EP  - 321
ER  - 

@article{
author = "Rajičić, Marija and Adnađević, Tanja and Stamenković, Gorana and Blagojević, Jelena and Vujošević, Mladen",
year = "2015",
abstract = "B chromosomes (Bs) are a very heterogeneous group of extra chromosomes.
   In various species Bs occur with different nucleotide sequences ranging
   from repetitive to protein coding. In yellow-necked field mice, Apodemus
   flavicollis Bs are small euchromatic chromosomes and untill now, only
   few molecular analyses have been conducted. In this study we examined A.
   flavicollis individuals with different number of Bs for presence of two
   genes, C-KIT and 18S rRNA. The C-KIT proto-oncogene was found on Bs in
   three Canidae species and one Cervidae species. This gene is a coding
   receptor critical for proliferation and cell differentiation of
   hematopoietic, melanoblast and primordial germ cells, and is highly
   conserved within mammals. While using semiquantitative PCR, we did not
   notice any difference in the C-KIT band intensity among animals with
   different number of Bs (0-3). The presence of only one copy of C-KIT
   gene was confirmed using real time-PCR on genomic DNA of A. flavicollis
   specimens with different number of Bs. rRNA genes in eukaryotes' genome
   are organized like units of tandem repeated sequences. The units form
   distinct clusters on one to several chromosome pairs. rRNA genes were
   found on Bs in different species including two species of genus
   Apodemus. One particular sample with 2 Bs showed the number of 18S rRNA
   gene about three times that of the calibrator 0 B sample. This result
   can indicate the presence of 18S rRNA gene on Bs, but its confirmation
   requires the implementation of other methods. Still, we can neither
   confirm nor deny the existence of pseudogen of tested target genes, or
   lose of exon 1 of C-KIT protooncogen in Bs of A. flavicollis. Our
   findings are further discussed.",
journal = "Genetika-Belgrade",
title = "Screening of B chromosomes for presence of two genes in yellow-necked mice, Apodemus flavicollis (Mammalia, Rodentia)",
number = "1",
volume = "47",
doi = "10.2298/GENSR1501311R",
pages = "311-321"
}

Rajičić, M., Adnađević, T., Stamenković, G., Blagojević, J.,& Vujošević, M.. (2015). Screening of B chromosomes for presence of two genes in yellow-necked mice, Apodemus flavicollis (Mammalia, Rodentia). in Genetika-Belgrade, 47(1), 311-321.
https://doi.org/10.2298/GENSR1501311R

Rajičić M, Adnađević T, Stamenković G, Blagojević J, Vujošević M. Screening of B chromosomes for presence of two genes in yellow-necked mice, Apodemus flavicollis (Mammalia, Rodentia). in Genetika-Belgrade. 2015;47(1):311-321.
doi:10.2298/GENSR1501311R .

Rajičić, Marija, Adnađević, Tanja, Stamenković, Gorana, Blagojević, Jelena, Vujošević, Mladen, "Screening of B chromosomes for presence of two genes in yellow-necked mice, Apodemus flavicollis (Mammalia, Rodentia)" in Genetika-Belgrade, 47, no. 1 (2015):311-321,
https://doi.org/10.2298/GENSR1501311R . .

TY  - JOUR
AU  - Adnađević, Tanja
AU  - Jovanović, Vladimir
AU  - Blagojević, Jelena
AU  - Budinski, Ivana
AU  - Cabrilo, Borislav
AU  - Bijelic-Cabrilo, Olivera
AU  - Vujošević, Mladen
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2124
AB  - Genetic background underlying wild populations immune response to
   different parasites is still not well understood. We studied immune
   response to multiple infections and to competition between different
   parasite species at different developmental stages in population of
   yellow-necked mouse, Apodemus flavicollis. Quantitative real-time PCR
   was used to investigate associations of MHC II-DRB, IL-10 and Tgf-beta
   genes expressions with presence of intestinal parasites at different
   developmental stages. Furthermore, we were interested whether the host
   related characteristics (sex, age, body condition, presence of B
   chromosomes or expression of other genes) or characteristics of present
   parasites (number of adult parasites of each identified species, egg
   count of each parasite genus, total number of nematode individuals)
   affect differential expression of the studied genes. A significant
   invert association between the expression of MHC II-DRB and Tgf-beta
   gene was found, which together with absence of IL-10 association
   confirmed modified Th2 as the main type of immune response to nematode
   infections. Effect of recorded parasites and parasite life-cycle stage
   on expression levels of MHC II-DRB gene was detected only through
   interactions with host-related characteristics such as sex, age, and the
   presence of B chromosomes. The presence of B chromosomes is associated
   with lower expression level of Tgf-beta gene. Although the influence of
   host genetic background on parasite infection has already been well
   documented, this is the first study in mammals that gave presence of B
   chromosomes on immune response full consideration.
T2  - Plos One
T1  - Possible Influence of B Chromosomes on Genes Included in Immune Response
 and Parasite Burden in Apodemus flavicollis
IS  - e112260
VL  - 9
DO  - 10.1371/journal.pone.0112260
ER  - 

@article{
author = "Adnađević, Tanja and Jovanović, Vladimir and Blagojević, Jelena and Budinski, Ivana and Cabrilo, Borislav and Bijelic-Cabrilo, Olivera and Vujošević, Mladen",
year = "2014",
abstract = "Genetic background underlying wild populations immune response to
   different parasites is still not well understood. We studied immune
   response to multiple infections and to competition between different
   parasite species at different developmental stages in population of
   yellow-necked mouse, Apodemus flavicollis. Quantitative real-time PCR
   was used to investigate associations of MHC II-DRB, IL-10 and Tgf-beta
   genes expressions with presence of intestinal parasites at different
   developmental stages. Furthermore, we were interested whether the host
   related characteristics (sex, age, body condition, presence of B
   chromosomes or expression of other genes) or characteristics of present
   parasites (number of adult parasites of each identified species, egg
   count of each parasite genus, total number of nematode individuals)
   affect differential expression of the studied genes. A significant
   invert association between the expression of MHC II-DRB and Tgf-beta
   gene was found, which together with absence of IL-10 association
   confirmed modified Th2 as the main type of immune response to nematode
   infections. Effect of recorded parasites and parasite life-cycle stage
   on expression levels of MHC II-DRB gene was detected only through
   interactions with host-related characteristics such as sex, age, and the
   presence of B chromosomes. The presence of B chromosomes is associated
   with lower expression level of Tgf-beta gene. Although the influence of
   host genetic background on parasite infection has already been well
   documented, this is the first study in mammals that gave presence of B
   chromosomes on immune response full consideration.",
journal = "Plos One",
title = "Possible Influence of B Chromosomes on Genes Included in Immune Response
 and Parasite Burden in Apodemus flavicollis",
number = "e112260",
volume = "9",
doi = "10.1371/journal.pone.0112260"
}

Adnađević, T., Jovanović, V., Blagojević, J., Budinski, I., Cabrilo, B., Bijelic-Cabrilo, O.,& Vujošević, M.. (2014). Possible Influence of B Chromosomes on Genes Included in Immune Response
 and Parasite Burden in Apodemus flavicollis. in Plos One, 9(e112260).
https://doi.org/10.1371/journal.pone.0112260

Adnađević T, Jovanović V, Blagojević J, Budinski I, Cabrilo B, Bijelic-Cabrilo O, Vujošević M. Possible Influence of B Chromosomes on Genes Included in Immune Response
 and Parasite Burden in Apodemus flavicollis. in Plos One. 2014;9(e112260).
doi:10.1371/journal.pone.0112260 .

Adnađević, Tanja, Jovanović, Vladimir, Blagojević, Jelena, Budinski, Ivana, Cabrilo, Borislav, Bijelic-Cabrilo, Olivera, Vujošević, Mladen, "Possible Influence of B Chromosomes on Genes Included in Immune Response
 and Parasite Burden in Apodemus flavicollis" in Plos One, 9, no. e112260 (2014),
https://doi.org/10.1371/journal.pone.0112260 . .

TY  - JOUR
AU  - Bugarski-Stanojević, Vanja
AU  - Blagojević, Jelena
AU  - Adnađević, Tanja
AU  - Jovanović, Vladimir
AU  - Vujošević, Mladen
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1053
AB  - The yellow-necked field mouse, Apodemus flavicollis (Melchior, 1934), and the long-tailed field mouse, Apodemus sylvaticus (Linnaeus, 1758) are morphologically similar species, with largely overlapping geographical areas and almost equal ecological requirements. This makes reliable species diagnosis based on external characters a real challenge. When advanced multivariate methods of skull and/or dental morphometrics are employed, specimens can be successfully distinguished in most cases, but not all, making single specimen identification impossible. Application of C-band karyotyping clearly distinguishes between these two species. However, it can be applied only to live animals. Several molecular methods have also allowed successful species diagnosis, but their universality has not been tested. Here we compare the diagnostic power of three molecular approaches and their applicability to populations from a species wide distributional area, as well as their simplicity of use, both for live animals and for samples stored in alcohol for a considerable period, without DNA sequencing. A total of 200 tissue samples from Morocco, France, Belgium, Serbia, Romania, Greece, Russia, Turkey and Kazakhstan, were analyzed by AP-PCR, ISSR-PCR and species-specific primers from the mitochondrial cytochrome b gene. The first two methods gave clear species-specific DNA profiles in complete agreement with previous C-band results. However, only partial agreement was observed between the third, species-specific primer method and the other two approaches and C-banding, as there were 4.5-10.5% false positive results. Hence we propose AP-PCR and ISSR-PCR techniques with particular chosen primers for quick and reliable diagnosis of these two species. A large number of samples can be assayed in a short period, with minimal cost and effort. Moreover, these techniques can be applied to a single specimen. (C) 2012 Elsevier GmbH. All rights reserved.
T2  - Zoologischer Anzeiger
T1  - Identification of the sibling species Apodemus sylvaticus and Apodemus flavicollis (Rodentia, Muridae)-Comparison of molecular methods
IS  - 4
VL  - 252
DO  - 10.1016/j.jcz.2012.11.004
SP  - 329
EP  - 587
ER  - 

@article{
author = "Bugarski-Stanojević, Vanja and Blagojević, Jelena and Adnađević, Tanja and Jovanović, Vladimir and Vujošević, Mladen",
year = "2013",
abstract = "The yellow-necked field mouse, Apodemus flavicollis (Melchior, 1934), and the long-tailed field mouse, Apodemus sylvaticus (Linnaeus, 1758) are morphologically similar species, with largely overlapping geographical areas and almost equal ecological requirements. This makes reliable species diagnosis based on external characters a real challenge. When advanced multivariate methods of skull and/or dental morphometrics are employed, specimens can be successfully distinguished in most cases, but not all, making single specimen identification impossible. Application of C-band karyotyping clearly distinguishes between these two species. However, it can be applied only to live animals. Several molecular methods have also allowed successful species diagnosis, but their universality has not been tested. Here we compare the diagnostic power of three molecular approaches and their applicability to populations from a species wide distributional area, as well as their simplicity of use, both for live animals and for samples stored in alcohol for a considerable period, without DNA sequencing. A total of 200 tissue samples from Morocco, France, Belgium, Serbia, Romania, Greece, Russia, Turkey and Kazakhstan, were analyzed by AP-PCR, ISSR-PCR and species-specific primers from the mitochondrial cytochrome b gene. The first two methods gave clear species-specific DNA profiles in complete agreement with previous C-band results. However, only partial agreement was observed between the third, species-specific primer method and the other two approaches and C-banding, as there were 4.5-10.5% false positive results. Hence we propose AP-PCR and ISSR-PCR techniques with particular chosen primers for quick and reliable diagnosis of these two species. A large number of samples can be assayed in a short period, with minimal cost and effort. Moreover, these techniques can be applied to a single specimen. (C) 2012 Elsevier GmbH. All rights reserved.",
journal = "Zoologischer Anzeiger",
title = "Identification of the sibling species Apodemus sylvaticus and Apodemus flavicollis (Rodentia, Muridae)-Comparison of molecular methods",
number = "4",
volume = "252",
doi = "10.1016/j.jcz.2012.11.004",
pages = "329-587"
}

Bugarski-Stanojević, V., Blagojević, J., Adnađević, T., Jovanović, V.,& Vujošević, M.. (2013). Identification of the sibling species Apodemus sylvaticus and Apodemus flavicollis (Rodentia, Muridae)-Comparison of molecular methods. in Zoologischer Anzeiger, 252(4), 329-587.
https://doi.org/10.1016/j.jcz.2012.11.004

Bugarski-Stanojević V, Blagojević J, Adnađević T, Jovanović V, Vujošević M. Identification of the sibling species Apodemus sylvaticus and Apodemus flavicollis (Rodentia, Muridae)-Comparison of molecular methods. in Zoologischer Anzeiger. 2013;252(4):329-587.
doi:10.1016/j.jcz.2012.11.004 .

Bugarski-Stanojević, Vanja, Blagojević, Jelena, Adnađević, Tanja, Jovanović, Vladimir, Vujošević, Mladen, "Identification of the sibling species Apodemus sylvaticus and Apodemus flavicollis (Rodentia, Muridae)-Comparison of molecular methods" in Zoologischer Anzeiger, 252, no. 4 (2013):329-587,
https://doi.org/10.1016/j.jcz.2012.11.004 . .

TY  - JOUR
AU  - Blagojević, Jelena
AU  - Jovanović, Vladimir
AU  - Adnađević, Tanja
AU  - Budinski, Ivana
AU  - Vujošević, Mladen
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/451
AB  - Marsh marigold, Caltha palustris is distributed in the moist, temperate and cold regions of the Northern Hemisphere. This species exhibits considerable amount of intraspecific chromosomal diversity involving hybridization, polyploidy, aneuploidy and B chromosomes. Karyotype analyses of three mountain populations from Serbia were done for the first time. All samples were tetraploid (based number x=8) with 2n=32. In population from mountain Tara presence of one B chromosomes was detected. Tetraploid karyotype consists of 17 median-centromeric (m), 8 submedian-centromeric (sm), 7 subterminal-centromeric (st) chromosomes and one terminal-centromeric (t) B chromosome (2n= 17m+8sm+7st+1B). Studied populations in Serbia belong to the most common cytotype for this species in Europe.
AB  - Kaljužnica, Caltha palustris, naseljava vlažne, umerene i hladne predele severne hemisfere. Ovu vrstu karakteriše znatna količina intraspecijske hromozomske varijabilnosti uključujući hibridizaciju, poliploidiju, aneuploidiju i B hromozome. Po prvi put je urađena kariotipska analiza tri planinske populacije iz Srbije. Svi analizirani uzorci su bili tetraploidi (osnovni broj n=8) sa 2n=32. U populaciji sa planine Tare detektovano je prisustvo jednog B hromozoma. Tetraploidni kariotip se sastojao od 17 metacentričnih (m), 8 submetacentričnih (sm), 7 subtelocentričnih (st) i jednog telocentričnog (t) B hromozoma (2n=17m+8sm+7st+1B). Analizirane populacije iz Srbije pripadaju citotipu koji je najčešći za ovu vrstu u Evropi.
T2  - Genetika
T1  - Hromozomski status kaljužnice, Caltha palustris L. (Ranunculaceae) iz Srbije
T1  - Chromosome status of marsh marigold, Caltha palustris L. (Ranunculaceae) from Serbia
IS  - 3
VL  - 45
SP  - 793
EP  - 798
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_451
ER  - 

@article{
author = "Blagojević, Jelena and Jovanović, Vladimir and Adnađević, Tanja and Budinski, Ivana and Vujošević, Mladen",
year = "2013, 2013",
abstract = "Marsh marigold, Caltha palustris is distributed in the moist, temperate and cold regions of the Northern Hemisphere. This species exhibits considerable amount of intraspecific chromosomal diversity involving hybridization, polyploidy, aneuploidy and B chromosomes. Karyotype analyses of three mountain populations from Serbia were done for the first time. All samples were tetraploid (based number x=8) with 2n=32. In population from mountain Tara presence of one B chromosomes was detected. Tetraploid karyotype consists of 17 median-centromeric (m), 8 submedian-centromeric (sm), 7 subterminal-centromeric (st) chromosomes and one terminal-centromeric (t) B chromosome (2n= 17m+8sm+7st+1B). Studied populations in Serbia belong to the most common cytotype for this species in Europe., Kaljužnica, Caltha palustris, naseljava vlažne, umerene i hladne predele severne hemisfere. Ovu vrstu karakteriše znatna količina intraspecijske hromozomske varijabilnosti uključujući hibridizaciju, poliploidiju, aneuploidiju i B hromozome. Po prvi put je urađena kariotipska analiza tri planinske populacije iz Srbije. Svi analizirani uzorci su bili tetraploidi (osnovni broj n=8) sa 2n=32. U populaciji sa planine Tare detektovano je prisustvo jednog B hromozoma. Tetraploidni kariotip se sastojao od 17 metacentričnih (m), 8 submetacentričnih (sm), 7 subtelocentričnih (st) i jednog telocentričnog (t) B hromozoma (2n=17m+8sm+7st+1B). Analizirane populacije iz Srbije pripadaju citotipu koji je najčešći za ovu vrstu u Evropi.",
journal = "Genetika",
title = "Hromozomski status kaljužnice, Caltha palustris L. (Ranunculaceae) iz Srbije, Chromosome status of marsh marigold, Caltha palustris L. (Ranunculaceae) from Serbia",
number = "3",
volume = "45",
pages = "793-798",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_451"
}

Blagojević, J., Jovanović, V., Adnađević, T., Budinski, I.,& Vujošević, M.. (2013). Hromozomski status kaljužnice, Caltha palustris L. (Ranunculaceae) iz Srbije. in Genetika, 45(3), 793-798.
https://hdl.handle.net/21.15107/rcub_ibiss_451

Blagojević J, Jovanović V, Adnađević T, Budinski I, Vujošević M. Hromozomski status kaljužnice, Caltha palustris L. (Ranunculaceae) iz Srbije. in Genetika. 2013;45(3):793-798.
https://hdl.handle.net/21.15107/rcub_ibiss_451 .

Blagojević, Jelena, Jovanović, Vladimir, Adnađević, Tanja, Budinski, Ivana, Vujošević, Mladen, "Hromozomski status kaljužnice, Caltha palustris L. (Ranunculaceae) iz Srbije" in Genetika, 45, no. 3 (2013):793-798,
https://hdl.handle.net/21.15107/rcub_ibiss_451 .

TY  - JOUR
AU  - Adnađević, Tanja
AU  - Stamenković, Gorana
AU  - Bugarski-Stanojević, Vanja
AU  - Blagojević, Jelena
AU  - Vujošević, Mladen
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1105
AB  - Two alternative models are used to explain maintenance of polymorphism of B chromosomes (Bs) in populations of a great number of species. The parasitic model suggests deleterious effects of Bs on fitness of carriers, while the heterotic model assumes that, in the absence of drive, equilibrium is produced by beneficial effects of Bs at low numbers. In order to determine the potential contribution of Bs to genetic differentiation and diversity, four populations of Apodemus flavicollis, differing in frequency of Bs (from 0.23 to 0.38) and settled in ecologically different habitats, were analyzed by 471 AFLP markers. Although numerous loci were demonstrated to be population specific, none of them was associated with individuals with Bs. AMOVA showed that the presence of Bs does not affect population differentiation, pointing to greater genetic similarity of Bs to A chromosomes. The greatest genetic diversity (0.241) was found in the population settled in optimal conditions for this species featured by the lowest frequency of animals with Bs (0.23). We found that the majority of loci marked as loci under directional selection, are characteristic of either a population with lower or one with a higher frequency of Bs. Several loci detected as outliers were associated with environmental variables that could directly and/or indirectly influence population dynamics of A. flavicollis. Thus, we suggest that the different frequency of Bs carriers in populations is related to adaptive differentiation to diverse habitats, which is in accordance with the heterotic model of Bs maintenance.
T2  - Population Ecology
T1  - Genetic differentiation in populations of the yellow-necked mouse, Apodemus flavicollis, harbouring B chromosomes in different frequencies
IS  - 4
VL  - 54
DO  - 10.1007/s10144-012-0333-6
SP  - 7
EP  - 548
ER  - 

@article{
author = "Adnađević, Tanja and Stamenković, Gorana and Bugarski-Stanojević, Vanja and Blagojević, Jelena and Vujošević, Mladen",
year = "2012",
abstract = "Two alternative models are used to explain maintenance of polymorphism of B chromosomes (Bs) in populations of a great number of species. The parasitic model suggests deleterious effects of Bs on fitness of carriers, while the heterotic model assumes that, in the absence of drive, equilibrium is produced by beneficial effects of Bs at low numbers. In order to determine the potential contribution of Bs to genetic differentiation and diversity, four populations of Apodemus flavicollis, differing in frequency of Bs (from 0.23 to 0.38) and settled in ecologically different habitats, were analyzed by 471 AFLP markers. Although numerous loci were demonstrated to be population specific, none of them was associated with individuals with Bs. AMOVA showed that the presence of Bs does not affect population differentiation, pointing to greater genetic similarity of Bs to A chromosomes. The greatest genetic diversity (0.241) was found in the population settled in optimal conditions for this species featured by the lowest frequency of animals with Bs (0.23). We found that the majority of loci marked as loci under directional selection, are characteristic of either a population with lower or one with a higher frequency of Bs. Several loci detected as outliers were associated with environmental variables that could directly and/or indirectly influence population dynamics of A. flavicollis. Thus, we suggest that the different frequency of Bs carriers in populations is related to adaptive differentiation to diverse habitats, which is in accordance with the heterotic model of Bs maintenance.",
journal = "Population Ecology",
title = "Genetic differentiation in populations of the yellow-necked mouse, Apodemus flavicollis, harbouring B chromosomes in different frequencies",
number = "4",
volume = "54",
doi = "10.1007/s10144-012-0333-6",
pages = "7-548"
}

Adnađević, T., Stamenković, G., Bugarski-Stanojević, V., Blagojević, J.,& Vujošević, M.. (2012). Genetic differentiation in populations of the yellow-necked mouse, Apodemus flavicollis, harbouring B chromosomes in different frequencies. in Population Ecology, 54(4), 7-548.
https://doi.org/10.1007/s10144-012-0333-6

Adnađević T, Stamenković G, Bugarski-Stanojević V, Blagojević J, Vujošević M. Genetic differentiation in populations of the yellow-necked mouse, Apodemus flavicollis, harbouring B chromosomes in different frequencies. in Population Ecology. 2012;54(4):7-548.
doi:10.1007/s10144-012-0333-6 .

Adnađević, Tanja, Stamenković, Gorana, Bugarski-Stanojević, Vanja, Blagojević, Jelena, Vujošević, Mladen, "Genetic differentiation in populations of the yellow-necked mouse, Apodemus flavicollis, harbouring B chromosomes in different frequencies" in Population Ecology, 54, no. 4 (2012):7-548,
https://doi.org/10.1007/s10144-012-0333-6 . .

TY  - JOUR
AU  - Blagojević, Jelena
AU  - Jovanović, Vladimir
AU  - Stamenković, Gorana
AU  - Jojić, Vida
AU  - Bugarski-Stanojević, Vanja
AU  - Adnađević, Tanja
AU  - Vujošević, Mladen
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4648
AB  - Bioaccumulation of heavy metals in the skulls of black-striped field mice (Apodemusagrarius)
was compared for two localities in Serbia differing in the level of pollution. Eight heavy metals: Fe, Mn, Co,
Cd, Zn, Ni, Pb and Cu, were quantitatively analyzed by atomic absorption spectrophotometry.Four of them
(Mn, Cd, Fe and Ni) showed significantly higher concentrations at the polluted location than in the relatively
unpolluted one. Concentrations of heavy metals in three age categories exhibited opposite patterns depending
on pollution levels. At the unpolluted locality heavy metal concentrations were the highest in the youngest
group and lower in older animals. In contrast, bone metal concentrations increased with age class at the more
polluted site. At the same time, we found that young animals from the polluted location had a statistically
significant lower relative body mass (RBM) than those from the unpolluted area(t = 7.155, p < 0.001), which
may have been caused by heavy metals or other factors associated with pollution. In general, we found that age
is a critical factor for estimating the level of heavy metal pollution, so proper investigations should account for
the age structure of the samples.
PB  - Tehran: University of Tehran, Graduate Faculty of Environment
T2  - International Journal of Environmental Research
T1  - Age Differences in Bioaccumulation of Heavy Metals in Populations of the Black-Striped Field Mouse, Apodemusagrarius(Rodentia, Mammalia)
IS  - 4
VL  - 6
DO  - 10.22059/ijer.2012.575
SP  - 1045
EP  - 1052
ER  - 

@article{
author = "Blagojević, Jelena and Jovanović, Vladimir and Stamenković, Gorana and Jojić, Vida and Bugarski-Stanojević, Vanja and Adnađević, Tanja and Vujošević, Mladen",
year = "2012",
abstract = "Bioaccumulation of heavy metals in the skulls of black-striped field mice (Apodemusagrarius)
was compared for two localities in Serbia differing in the level of pollution. Eight heavy metals: Fe, Mn, Co,
Cd, Zn, Ni, Pb and Cu, were quantitatively analyzed by atomic absorption spectrophotometry.Four of them
(Mn, Cd, Fe and Ni) showed significantly higher concentrations at the polluted location than in the relatively
unpolluted one. Concentrations of heavy metals in three age categories exhibited opposite patterns depending
on pollution levels. At the unpolluted locality heavy metal concentrations were the highest in the youngest
group and lower in older animals. In contrast, bone metal concentrations increased with age class at the more
polluted site. At the same time, we found that young animals from the polluted location had a statistically
significant lower relative body mass (RBM) than those from the unpolluted area(t = 7.155, p < 0.001), which
may have been caused by heavy metals or other factors associated with pollution. In general, we found that age
is a critical factor for estimating the level of heavy metal pollution, so proper investigations should account for
the age structure of the samples.",
publisher = "Tehran: University of Tehran, Graduate Faculty of Environment",
journal = "International Journal of Environmental Research",
title = "Age Differences in Bioaccumulation of Heavy Metals in Populations of the Black-Striped Field Mouse, Apodemusagrarius(Rodentia, Mammalia)",
number = "4",
volume = "6",
doi = "10.22059/ijer.2012.575",
pages = "1045-1052"
}

Blagojević, J., Jovanović, V., Stamenković, G., Jojić, V., Bugarski-Stanojević, V., Adnađević, T.,& Vujošević, M.. (2012). Age Differences in Bioaccumulation of Heavy Metals in Populations of the Black-Striped Field Mouse, Apodemusagrarius(Rodentia, Mammalia). in International Journal of Environmental Research
Tehran: University of Tehran, Graduate Faculty of Environment., 6(4), 1045-1052.
https://doi.org/10.22059/ijer.2012.575

Blagojević J, Jovanović V, Stamenković G, Jojić V, Bugarski-Stanojević V, Adnađević T, Vujošević M. Age Differences in Bioaccumulation of Heavy Metals in Populations of the Black-Striped Field Mouse, Apodemusagrarius(Rodentia, Mammalia). in International Journal of Environmental Research. 2012;6(4):1045-1052.
doi:10.22059/ijer.2012.575 .

Blagojević, Jelena, Jovanović, Vladimir, Stamenković, Gorana, Jojić, Vida, Bugarski-Stanojević, Vanja, Adnađević, Tanja, Vujošević, Mladen, "Age Differences in Bioaccumulation of Heavy Metals in Populations of the Black-Striped Field Mouse, Apodemusagrarius(Rodentia, Mammalia)" in International Journal of Environmental Research, 6, no. 4 (2012):1045-1052,
https://doi.org/10.22059/ijer.2012.575 . .

TY  - JOUR
AU  - Bugarski-Stanojević, Vanja
AU  - Blagojević, Jelena
AU  - Stamenković, Gorana
AU  - Adnađević, Tanja
AU  - Giagia-Athanasopoulou, Eva B
AU  - Vujošević, Mladen
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1316
AB  - The utility of the Inter Simple Sequence Repeat-Polymerase Chain Reaction (ISSR-PCR) was explored in order to determine genetic variation in six species of the genus Apodemus (A. flavicollis, A. sylvaticus, A. uralensis, A. agrarius, A. mystacinus and A. epimelas) at the individual level, population level, in separate geographic samples and in the species as a whole. Six optimized primers produced highly reproducible and polymorphic DNA markers with 98.3% polymorphic bands on a total sample of 91 individuals from 32 localities in Europe and Asia. Moreover, each primer allowed for an exact diagnosis of each of the six Apodemus species and thus provides a simple and reliable tool for the hitherto problematic discrimination of species from the subgenus Sylvaemus. Genetic distances between species ranged from 0.079, among the closely related A. flavicollis and A. sylvaticus, to 0.203 between A. mystacinus and A. agrarius. A. flavicollis, A. uralensis and A. sylvaticus display a strong population substructure. The range of genetic distances among geographic samples within last two species reaches the values obtained for closely related species. ISSR markers proved to be a simple and reliable tool for species diagnosis, as well as for estimating genetic diversity below the species level and for closely related species, but they showed questionable reliability for larger genetic distances.
T2  - Systematics and Biodiversity
T1  - Comparative study of the phylogenetic structure in six Apodemus species (Mammalia, Rodentia) inferred from ISSR-PCR data
IS  - 1
VL  - 9
DO  - 10.1080/14772000.2011.560970
EP  - 106
ER  - 

@article{
author = "Bugarski-Stanojević, Vanja and Blagojević, Jelena and Stamenković, Gorana and Adnađević, Tanja and Giagia-Athanasopoulou, Eva B and Vujošević, Mladen",
year = "2011",
abstract = "The utility of the Inter Simple Sequence Repeat-Polymerase Chain Reaction (ISSR-PCR) was explored in order to determine genetic variation in six species of the genus Apodemus (A. flavicollis, A. sylvaticus, A. uralensis, A. agrarius, A. mystacinus and A. epimelas) at the individual level, population level, in separate geographic samples and in the species as a whole. Six optimized primers produced highly reproducible and polymorphic DNA markers with 98.3% polymorphic bands on a total sample of 91 individuals from 32 localities in Europe and Asia. Moreover, each primer allowed for an exact diagnosis of each of the six Apodemus species and thus provides a simple and reliable tool for the hitherto problematic discrimination of species from the subgenus Sylvaemus. Genetic distances between species ranged from 0.079, among the closely related A. flavicollis and A. sylvaticus, to 0.203 between A. mystacinus and A. agrarius. A. flavicollis, A. uralensis and A. sylvaticus display a strong population substructure. The range of genetic distances among geographic samples within last two species reaches the values obtained for closely related species. ISSR markers proved to be a simple and reliable tool for species diagnosis, as well as for estimating genetic diversity below the species level and for closely related species, but they showed questionable reliability for larger genetic distances.",
journal = "Systematics and Biodiversity",
title = "Comparative study of the phylogenetic structure in six Apodemus species (Mammalia, Rodentia) inferred from ISSR-PCR data",
number = "1",
volume = "9",
doi = "10.1080/14772000.2011.560970",
pages = "106"
}

Bugarski-Stanojević, V., Blagojević, J., Stamenković, G., Adnađević, T., Giagia-Athanasopoulou, E. B.,& Vujošević, M.. (2011). Comparative study of the phylogenetic structure in six Apodemus species (Mammalia, Rodentia) inferred from ISSR-PCR data. in Systematics and Biodiversity, 9(1).
https://doi.org/10.1080/14772000.2011.560970

Bugarski-Stanojević V, Blagojević J, Stamenković G, Adnađević T, Giagia-Athanasopoulou EB, Vujošević M. Comparative study of the phylogenetic structure in six Apodemus species (Mammalia, Rodentia) inferred from ISSR-PCR data. in Systematics and Biodiversity. 2011;9(1):null-106.
doi:10.1080/14772000.2011.560970 .

Bugarski-Stanojević, Vanja, Blagojević, Jelena, Stamenković, Gorana, Adnađević, Tanja, Giagia-Athanasopoulou, Eva B, Vujošević, Mladen, "Comparative study of the phylogenetic structure in six Apodemus species (Mammalia, Rodentia) inferred from ISSR-PCR data" in Systematics and Biodiversity, 9, no. 1 (2011),
https://doi.org/10.1080/14772000.2011.560970 . .

TY  - JOUR
AU  - Vujošević, Mladen
AU  - Blagojević, Jelena
AU  - Jojić, Vida
AU  - Bugarski-Stanojević, Vanja
AU  - Adnađević, Tanja
AU  - Stamenković, Gorana
PY  - 2009
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/236
AB  - The presence of B chromosomes (Bs) is frequently found in populations of yellow-necked mouse, Apodemus flavicollis. Differences in frequencies of Bs in age categories were analyzed in 717 animals in order to clarify the mechanisms of their maintenance in populations of A. flavicollis. The absence of statistically significant differences in the frequency of Bs between six age categories indicates that the maintenance of Bs in populations can be explained by in terms of their contribution to overall genetic diversity of the species rather than by parasitic behavior.
AB  - Prekobrojni hromozomi nazivaju se B hromozomi ako zadovoljavaju tri kriterijuma: nisu neophodni za preživljavanje, ne sparuju se sa hromozomima A seta i ne nasleđuju se mendelovski. B hromozomi su vrlo često prisutni u populacijama žutogrlog miša, Apodemus flavicollis. Njihovo održavanje u populacijama objašnjava se sa dva suprostavljena modela. Model označen kao parazitski tvrdi da se B hromozomi održavaju zahvaljujući mehanizmu akumulacije uprkos štetnim efektima na fitnes nosilaca. S druge strane, heterotički model sugeriše da u odsustvu akumulacije mali broj B hromozoma ima adaptivnu prednost za nosioce, dok veći broj može biti štetan. Ako B hromozomi, koji su označeni kao parazitski, redukuju fitnes kod jedinki koje su njihovi nosioci, onda je očekivano da frekvenca jedinki sa B hromozomima opada sa starošću. Analizirana je razlika u frekvenci B hromozoma u uzrasnim kategorijama kod 717 jedinki sa ciljem da se razjasne mehanizmi njihovog održavanja u populacijama A. flavicollis. Odsustvo statistički značajnih razlika u frekvenci B hromozoma između 6 uzrasnih kategorija ukazuje da se održavanje B hromozoma u populacijama ove vrste može objasniti njihovim doprinosom ukupnoj genetičkoj raznovrsnosti pre nego parazitskim ponašanjem.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Distribucija B hromozoma u uzrasnim kategorijama žutogrlog miša, Apodemus flavicollis (Mammalia, Rodentia)
T1  - Distribution of B chromosomes in age categories of the yellow-necked mouse Apodemus flavicollis (Mammalia, Rodentia)
IS  - 4
VL  - 61
DO  - 10.2298/ABS0904653V
SP  - 653
EP  - 658
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_236
ER  - 

@article{
author = "Vujošević, Mladen and Blagojević, Jelena and Jojić, Vida and Bugarski-Stanojević, Vanja and Adnađević, Tanja and Stamenković, Gorana",
year = "2009, 2009",
abstract = "The presence of B chromosomes (Bs) is frequently found in populations of yellow-necked mouse, Apodemus flavicollis. Differences in frequencies of Bs in age categories were analyzed in 717 animals in order to clarify the mechanisms of their maintenance in populations of A. flavicollis. The absence of statistically significant differences in the frequency of Bs between six age categories indicates that the maintenance of Bs in populations can be explained by in terms of their contribution to overall genetic diversity of the species rather than by parasitic behavior., Prekobrojni hromozomi nazivaju se B hromozomi ako zadovoljavaju tri kriterijuma: nisu neophodni za preživljavanje, ne sparuju se sa hromozomima A seta i ne nasleđuju se mendelovski. B hromozomi su vrlo često prisutni u populacijama žutogrlog miša, Apodemus flavicollis. Njihovo održavanje u populacijama objašnjava se sa dva suprostavljena modela. Model označen kao parazitski tvrdi da se B hromozomi održavaju zahvaljujući mehanizmu akumulacije uprkos štetnim efektima na fitnes nosilaca. S druge strane, heterotički model sugeriše da u odsustvu akumulacije mali broj B hromozoma ima adaptivnu prednost za nosioce, dok veći broj može biti štetan. Ako B hromozomi, koji su označeni kao parazitski, redukuju fitnes kod jedinki koje su njihovi nosioci, onda je očekivano da frekvenca jedinki sa B hromozomima opada sa starošću. Analizirana je razlika u frekvenci B hromozoma u uzrasnim kategorijama kod 717 jedinki sa ciljem da se razjasne mehanizmi njihovog održavanja u populacijama A. flavicollis. Odsustvo statistički značajnih razlika u frekvenci B hromozoma između 6 uzrasnih kategorija ukazuje da se održavanje B hromozoma u populacijama ove vrste može objasniti njihovim doprinosom ukupnoj genetičkoj raznovrsnosti pre nego parazitskim ponašanjem.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Distribucija B hromozoma u uzrasnim kategorijama žutogrlog miša, Apodemus flavicollis (Mammalia, Rodentia), Distribution of B chromosomes in age categories of the yellow-necked mouse Apodemus flavicollis (Mammalia, Rodentia)",
number = "4",
volume = "61",
doi = "10.2298/ABS0904653V",
pages = "653-658",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_236"
}

Vujošević, M., Blagojević, J., Jojić, V., Bugarski-Stanojević, V., Adnađević, T.,& Stamenković, G.. (2009). Distribucija B hromozoma u uzrasnim kategorijama žutogrlog miša, Apodemus flavicollis (Mammalia, Rodentia). in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 61(4), 653-658.
https://doi.org/10.2298/ABS0904653V
https://hdl.handle.net/21.15107/rcub_ibiss_236

Vujošević M, Blagojević J, Jojić V, Bugarski-Stanojević V, Adnađević T, Stamenković G. Distribucija B hromozoma u uzrasnim kategorijama žutogrlog miša, Apodemus flavicollis (Mammalia, Rodentia). in Archives of Biological Sciences. 2009;61(4):653-658.
doi:10.2298/ABS0904653V
https://hdl.handle.net/21.15107/rcub_ibiss_236 .

Vujošević, Mladen, Blagojević, Jelena, Jojić, Vida, Bugarski-Stanojević, Vanja, Adnađević, Tanja, Stamenković, Gorana, "Distribucija B hromozoma u uzrasnim kategorijama žutogrlog miša, Apodemus flavicollis (Mammalia, Rodentia)" in Archives of Biological Sciences, 61, no. 4 (2009):653-658,
https://doi.org/10.2298/ABS0904653V .,
https://hdl.handle.net/21.15107/rcub_ibiss_236 .