TY  - JOUR
AU  - Tagkouli, Dimitra
AU  - Tsiaka, Thalia
AU  - Kritsi, Eftichia
AU  - Soković, Marina
AU  - Sinanoglou, Vassilia J.
AU  - Lantzouraki, Dimitra Z.
AU  - Zoumpoulakis, Panagiotis
PY  - 2022
UR  - https://www.mdpi.com/1420-3049/27/7/2189
UR  - http://www.ncbi.nlm.nih.gov/pubmed/35408586
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9000764
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4951
AB  - Wine lees, a sub-exploited byproduct of vinification, is considered a rich source of bioactive compounds, such as (poly)phenols, anthocyanins and tannins. Thus, the effective and rapid recovery of these biomolecules and the assessment of the bioactive properties of wine lees extracts is of utmost importance. Towards this direction, microwave-assisted extraction (MAE) factors (i.e., extraction time, microwave power and solvent/material ratio) were optimized using experimental design models in order to maximize the (poly)phenolic yield of the extracts. After optimizing the MAE process, the total phenolic content (TPC) as well as the antiradical, antioxidant and antimicrobial activity of the extracts were evaluated. Furthermore, Fourier transform infrared spectroscopy (FTIR) was employed to investigate the chemical profile of wine lees extracts. Red varieties exhibited higher biological activity than white varieties. The geographical origin and fermentation stage were also considered as critical factors. The white variety Moschofilero presented the highest antioxidant, antiradical and antimicrobial activity, while Merlot and Agiorgitiko samples showed noteworthy activities among red varieties. Moreover, IR spectra confirmed the presence of sugars, amino acids, organic acids and aromatic compounds. Thus, an efficient, rapid and eco-friendly process was proposed for further valorization of wine lees extracts.
PB  - Basel: MDPI
T2  - Molecules (Basel, Switzerland)
T1  - Towards the Optimization of Microwave-Assisted Extraction and the Assessment of Chemical Profile, Antioxidant and Antimicrobial Activity of Wine Lees Extracts.
IS  - 7
VL  - 27
DO  - 10.3390/molecules27072189
SP  - 2189
ER  - 

@article{
author = "Tagkouli, Dimitra and Tsiaka, Thalia and Kritsi, Eftichia and Soković, Marina and Sinanoglou, Vassilia J. and Lantzouraki, Dimitra Z. and Zoumpoulakis, Panagiotis",
year = "2022",
abstract = "Wine lees, a sub-exploited byproduct of vinification, is considered a rich source of bioactive compounds, such as (poly)phenols, anthocyanins and tannins. Thus, the effective and rapid recovery of these biomolecules and the assessment of the bioactive properties of wine lees extracts is of utmost importance. Towards this direction, microwave-assisted extraction (MAE) factors (i.e., extraction time, microwave power and solvent/material ratio) were optimized using experimental design models in order to maximize the (poly)phenolic yield of the extracts. After optimizing the MAE process, the total phenolic content (TPC) as well as the antiradical, antioxidant and antimicrobial activity of the extracts were evaluated. Furthermore, Fourier transform infrared spectroscopy (FTIR) was employed to investigate the chemical profile of wine lees extracts. Red varieties exhibited higher biological activity than white varieties. The geographical origin and fermentation stage were also considered as critical factors. The white variety Moschofilero presented the highest antioxidant, antiradical and antimicrobial activity, while Merlot and Agiorgitiko samples showed noteworthy activities among red varieties. Moreover, IR spectra confirmed the presence of sugars, amino acids, organic acids and aromatic compounds. Thus, an efficient, rapid and eco-friendly process was proposed for further valorization of wine lees extracts.",
publisher = "Basel: MDPI",
journal = "Molecules (Basel, Switzerland)",
title = "Towards the Optimization of Microwave-Assisted Extraction and the Assessment of Chemical Profile, Antioxidant and Antimicrobial Activity of Wine Lees Extracts.",
number = "7",
volume = "27",
doi = "10.3390/molecules27072189",
pages = "2189"
}

Tagkouli, D., Tsiaka, T., Kritsi, E., Soković, M., Sinanoglou, V. J., Lantzouraki, D. Z.,& Zoumpoulakis, P.. (2022). Towards the Optimization of Microwave-Assisted Extraction and the Assessment of Chemical Profile, Antioxidant and Antimicrobial Activity of Wine Lees Extracts.. in Molecules (Basel, Switzerland)
Basel: MDPI., 27(7), 2189.
https://doi.org/10.3390/molecules27072189

Tagkouli D, Tsiaka T, Kritsi E, Soković M, Sinanoglou VJ, Lantzouraki DZ, Zoumpoulakis P. Towards the Optimization of Microwave-Assisted Extraction and the Assessment of Chemical Profile, Antioxidant and Antimicrobial Activity of Wine Lees Extracts.. in Molecules (Basel, Switzerland). 2022;27(7):2189.
doi:10.3390/molecules27072189 .

Tagkouli, Dimitra, Tsiaka, Thalia, Kritsi, Eftichia, Soković, Marina, Sinanoglou, Vassilia J., Lantzouraki, Dimitra Z., Zoumpoulakis, Panagiotis, "Towards the Optimization of Microwave-Assisted Extraction and the Assessment of Chemical Profile, Antioxidant and Antimicrobial Activity of Wine Lees Extracts." in Molecules (Basel, Switzerland), 27, no. 7 (2022):2189,
https://doi.org/10.3390/molecules27072189 . .

TY  - JOUR
AU  - Zoidis, Grigoris
AU  - Kritsi, Eftichia
AU  - Lecinska, Paulina
AU  - Ivanov, Marija
AU  - Zoumpoulakis, Panagiotis
AU  - Soković, Marina
AU  - Catto, Marco
PY  - 2021
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/cmdc.202000312
UR  - https://radar.ibiss.bg.ac.rs/123456789/3858
AB  - The significant antifungal activity of a series of novel 1,2,4-triazole derivatives against different strains of Candida albicans, Candida krusei and Aspergillus fumigatus, compared to the commercial fungicides ketoconazole and itraconazole, is reported. Systemic mycosis and invasive fungal infections, whether from immunodeficiency or hospital-acquired infection, have been on an upward trend for several years. The 1,2,4-triazole ring substituted with other aromatic and heteroaromatic systems plays an important role in the field of antifungal drug discovery and development. Thus, an extensive series of 29 triazoles, substituted in different positions with a variety of aromatic rings, has been designed, synthesized, and evaluated for their fungicidal activity. Almost all the agents tested in vitro showed high activity against all examined fungal strains. It is noteworthy that, in the case of A. fumigatus, all the examined compounds achieved equal or higher antifungal activity than ketoconazole, but less activity than itraconazole. Among all the derivatives studied, the dichlorourea analogue and bromo-substituted triazole stand out as the most promising compounds. Quantitative structure-activity relationship (QSAR) models were built for a systematic structure-activity relationship (SAR) profile to explain and potentially explore the potency characteristics of 1,2,4-triazole analogues.
PB  - John Wiley and Sons Ltd
T2  - ChemMedChem
T1  - The Triazole Ring as a Privileged Scaffold for Putative Antifungals: Synthesis and Evaluation of a Series of New Analogues
IS  - 1
VL  - 16
DO  - 10.1002/cmdc.202000312
SP  - cmdc.202000312
SP  - 134
EP  - 144
ER  - 

@article{
author = "Zoidis, Grigoris and Kritsi, Eftichia and Lecinska, Paulina and Ivanov, Marija and Zoumpoulakis, Panagiotis and Soković, Marina and Catto, Marco",
year = "2021",
abstract = "The significant antifungal activity of a series of novel 1,2,4-triazole derivatives against different strains of Candida albicans, Candida krusei and Aspergillus fumigatus, compared to the commercial fungicides ketoconazole and itraconazole, is reported. Systemic mycosis and invasive fungal infections, whether from immunodeficiency or hospital-acquired infection, have been on an upward trend for several years. The 1,2,4-triazole ring substituted with other aromatic and heteroaromatic systems plays an important role in the field of antifungal drug discovery and development. Thus, an extensive series of 29 triazoles, substituted in different positions with a variety of aromatic rings, has been designed, synthesized, and evaluated for their fungicidal activity. Almost all the agents tested in vitro showed high activity against all examined fungal strains. It is noteworthy that, in the case of A. fumigatus, all the examined compounds achieved equal or higher antifungal activity than ketoconazole, but less activity than itraconazole. Among all the derivatives studied, the dichlorourea analogue and bromo-substituted triazole stand out as the most promising compounds. Quantitative structure-activity relationship (QSAR) models were built for a systematic structure-activity relationship (SAR) profile to explain and potentially explore the potency characteristics of 1,2,4-triazole analogues.",
publisher = "John Wiley and Sons Ltd",
journal = "ChemMedChem",
title = "The Triazole Ring as a Privileged Scaffold for Putative Antifungals: Synthesis and Evaluation of a Series of New Analogues",
number = "1",
volume = "16",
doi = "10.1002/cmdc.202000312",
pages = "cmdc.202000312-134-144"
}

Zoidis, G., Kritsi, E., Lecinska, P., Ivanov, M., Zoumpoulakis, P., Soković, M.,& Catto, M.. (2021). The Triazole Ring as a Privileged Scaffold for Putative Antifungals: Synthesis and Evaluation of a Series of New Analogues. in ChemMedChem
John Wiley and Sons Ltd., 16(1), cmdc.202000312-144.
https://doi.org/10.1002/cmdc.202000312

Zoidis G, Kritsi E, Lecinska P, Ivanov M, Zoumpoulakis P, Soković M, Catto M. The Triazole Ring as a Privileged Scaffold for Putative Antifungals: Synthesis and Evaluation of a Series of New Analogues. in ChemMedChem. 2021;16(1):cmdc.202000312-144.
doi:10.1002/cmdc.202000312 .

Zoidis, Grigoris, Kritsi, Eftichia, Lecinska, Paulina, Ivanov, Marija, Zoumpoulakis, Panagiotis, Soković, Marina, Catto, Marco, "The Triazole Ring as a Privileged Scaffold for Putative Antifungals: Synthesis and Evaluation of a Series of New Analogues" in ChemMedChem, 16, no. 1 (2021):cmdc.202000312-144,
https://doi.org/10.1002/cmdc.202000312 . .

TY  - JOUR
AU  - Magoulas, George E.
AU  - Kalopetridou, Lefkothea
AU  - Ćirić, Ana
AU  - Kritsi, Eftichia
AU  - Kouka, Paraskevi
AU  - Zoumpoulakis, Panagiotis
AU  - Chondrogianni, Niki
AU  - Soković, Marina
AU  - Prousis, Kyriakos C.
AU  - Calogeropoulou, Theodora
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4078
AB  - A series of new thieno[2,3-d]pyrimidin-4(3H)-one derivatives were synthesized and evaluated for their activity against four gram-positive and four gram-negative bacterial and eight fungal species. The majority of the compounds exhibited excellent antimicrobial and antifungal activity, being more potent than the control compounds. Compound 22, bearing a m-methoxyphenyl group and an ethylenediamine side chain anchored at C-2 of the thienopyrimidinone core, is the most potent antibacterial compound with broad antimicrobial activity with MIC values in the range of 0.05–0.13 mM, being 6 to 15 fold more potent than the controls, streptomycin and ampicillin. Furthermore, compounds 14 and 15 which bear a p-chlorophenyl and m-methoxyphenyl group, respectively, and share a 2-(2-mercaptoethoxy)ethan-1-ol side chain showed the best antifungal activity, being 10–15 times more potent than ketoconazole or bifonazole with MIC values 0.013–0.026 and 0.027 mM, respectively. Especially in the case of compound 15 the low MIC values were accompanied by excellent MFC values ranging from 0.056 to 0.058 mM. Evaluation of toxicity in vitro on HFL-1 human embryonic primary cells and in vivo in the nematode C. elegans revealed no toxic effects for both compounds 15 and 22 tested at the MIC concentrations. Ligand-based similarity search and molecular docking predicted that the antibacterial activity of analogue 22 is related to inhibition of the topoisomerase II DNA gyrase enzyme and the antifungal activity of compound 15 to CYP51 lanosterol demethylase enzyme. R-Group analysis as a means of computational structure activity relationship tool, highlighted the compounds’ crucial pharmacophore features and their impact on the antibacterial and antifungal activity. The presence of a N-methyl piperidine ring fused to the thienopyrimidinone core plays an important role in both activities.
PB  - Academic Press Inc.
T2  - Bioorganic Chemistry
T1  - Synthesis, biological evaluation and QSAR studies of new thieno[2,3-d]pyrimidin-4(3H)-one derivatives as antimicrobial and antifungal agents
VL  - 106
DO  - 10.1016/j.bioorg.2020.104509
SP  - 104509
ER  - 

@article{
author = "Magoulas, George E. and Kalopetridou, Lefkothea and Ćirić, Ana and Kritsi, Eftichia and Kouka, Paraskevi and Zoumpoulakis, Panagiotis and Chondrogianni, Niki and Soković, Marina and Prousis, Kyriakos C. and Calogeropoulou, Theodora",
year = "2021",
abstract = "A series of new thieno[2,3-d]pyrimidin-4(3H)-one derivatives were synthesized and evaluated for their activity against four gram-positive and four gram-negative bacterial and eight fungal species. The majority of the compounds exhibited excellent antimicrobial and antifungal activity, being more potent than the control compounds. Compound 22, bearing a m-methoxyphenyl group and an ethylenediamine side chain anchored at C-2 of the thienopyrimidinone core, is the most potent antibacterial compound with broad antimicrobial activity with MIC values in the range of 0.05–0.13 mM, being 6 to 15 fold more potent than the controls, streptomycin and ampicillin. Furthermore, compounds 14 and 15 which bear a p-chlorophenyl and m-methoxyphenyl group, respectively, and share a 2-(2-mercaptoethoxy)ethan-1-ol side chain showed the best antifungal activity, being 10–15 times more potent than ketoconazole or bifonazole with MIC values 0.013–0.026 and 0.027 mM, respectively. Especially in the case of compound 15 the low MIC values were accompanied by excellent MFC values ranging from 0.056 to 0.058 mM. Evaluation of toxicity in vitro on HFL-1 human embryonic primary cells and in vivo in the nematode C. elegans revealed no toxic effects for both compounds 15 and 22 tested at the MIC concentrations. Ligand-based similarity search and molecular docking predicted that the antibacterial activity of analogue 22 is related to inhibition of the topoisomerase II DNA gyrase enzyme and the antifungal activity of compound 15 to CYP51 lanosterol demethylase enzyme. R-Group analysis as a means of computational structure activity relationship tool, highlighted the compounds’ crucial pharmacophore features and their impact on the antibacterial and antifungal activity. The presence of a N-methyl piperidine ring fused to the thienopyrimidinone core plays an important role in both activities.",
publisher = "Academic Press Inc.",
journal = "Bioorganic Chemistry",
title = "Synthesis, biological evaluation and QSAR studies of new thieno[2,3-d]pyrimidin-4(3H)-one derivatives as antimicrobial and antifungal agents",
volume = "106",
doi = "10.1016/j.bioorg.2020.104509",
pages = "104509"
}

Magoulas, G. E., Kalopetridou, L., Ćirić, A., Kritsi, E., Kouka, P., Zoumpoulakis, P., Chondrogianni, N., Soković, M., Prousis, K. C.,& Calogeropoulou, T.. (2021). Synthesis, biological evaluation and QSAR studies of new thieno[2,3-d]pyrimidin-4(3H)-one derivatives as antimicrobial and antifungal agents. in Bioorganic Chemistry
Academic Press Inc.., 106, 104509.
https://doi.org/10.1016/j.bioorg.2020.104509

Magoulas GE, Kalopetridou L, Ćirić A, Kritsi E, Kouka P, Zoumpoulakis P, Chondrogianni N, Soković M, Prousis KC, Calogeropoulou T. Synthesis, biological evaluation and QSAR studies of new thieno[2,3-d]pyrimidin-4(3H)-one derivatives as antimicrobial and antifungal agents. in Bioorganic Chemistry. 2021;106:104509.
doi:10.1016/j.bioorg.2020.104509 .

Magoulas, George E., Kalopetridou, Lefkothea, Ćirić, Ana, Kritsi, Eftichia, Kouka, Paraskevi, Zoumpoulakis, Panagiotis, Chondrogianni, Niki, Soković, Marina, Prousis, Kyriakos C., Calogeropoulou, Theodora, "Synthesis, biological evaluation and QSAR studies of new thieno[2,3-d]pyrimidin-4(3H)-one derivatives as antimicrobial and antifungal agents" in Bioorganic Chemistry, 106 (2021):104509,
https://doi.org/10.1016/j.bioorg.2020.104509 . .

TY  - JOUR
AU  - Tzani, Andromachi
AU  - Vaitsis, Christos
AU  - Kritsi, Eftichia
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Zoumpoulakis, Panagiotis
AU  - Detsi, Anastasia
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3662
AB  - In this work the effectiveness of two different reaction media, an Ionic Liquid (IL) and a Deep Eutectic Solvent (DES), as greener, alternative solvents for the synthesis of bioactive bis-(β-dicarbonyl)-methane derivatives is examined. A domino Knoevenagel-Michael reaction between selected aromatic aldehydes and heterocyclic 1,3-dicarbonyl compounds was successfully accomplished, producing the desired compounds in satisfactory yields. The solvents were recycled and reused three times without noticeable decrease in reaction yields. A putative conformation of compound 4g was determined using NMR spectroscopy and an “anti” orientation of the fused aromatic rings was proposed. Moreover, some of the bis-(β-dicarbonyl)-methane derivatives were tested for their antifungal activity against four Candida albicans strains. Biscoumarin 6 and bisquinolinone 4d exhibited promising anticandidial activity. In parallel, in silico ligand-based similarity calculations provided a putative mechanism of action of the examined compounds through CYP51 inhibition.
PB  - Elsevier B.V.
T2  - Journal of Molecular Structure
T1  - Green synthesis of bis-(β-dicarbonyl)-methane derivatives and biological evaluation as putative anticandidial agents
VL  - 1216
DO  - 10.1016/j.molstruc.2020.128276
SP  - 128276
ER  - 

@article{
author = "Tzani, Andromachi and Vaitsis, Christos and Kritsi, Eftichia and Ivanov, Marija and Soković, Marina and Zoumpoulakis, Panagiotis and Detsi, Anastasia",
year = "2020",
abstract = "In this work the effectiveness of two different reaction media, an Ionic Liquid (IL) and a Deep Eutectic Solvent (DES), as greener, alternative solvents for the synthesis of bioactive bis-(β-dicarbonyl)-methane derivatives is examined. A domino Knoevenagel-Michael reaction between selected aromatic aldehydes and heterocyclic 1,3-dicarbonyl compounds was successfully accomplished, producing the desired compounds in satisfactory yields. The solvents were recycled and reused three times without noticeable decrease in reaction yields. A putative conformation of compound 4g was determined using NMR spectroscopy and an “anti” orientation of the fused aromatic rings was proposed. Moreover, some of the bis-(β-dicarbonyl)-methane derivatives were tested for their antifungal activity against four Candida albicans strains. Biscoumarin 6 and bisquinolinone 4d exhibited promising anticandidial activity. In parallel, in silico ligand-based similarity calculations provided a putative mechanism of action of the examined compounds through CYP51 inhibition.",
publisher = "Elsevier B.V.",
journal = "Journal of Molecular Structure",
title = "Green synthesis of bis-(β-dicarbonyl)-methane derivatives and biological evaluation as putative anticandidial agents",
volume = "1216",
doi = "10.1016/j.molstruc.2020.128276",
pages = "128276"
}

Tzani, A., Vaitsis, C., Kritsi, E., Ivanov, M., Soković, M., Zoumpoulakis, P.,& Detsi, A.. (2020). Green synthesis of bis-(β-dicarbonyl)-methane derivatives and biological evaluation as putative anticandidial agents. in Journal of Molecular Structure
Elsevier B.V.., 1216, 128276.
https://doi.org/10.1016/j.molstruc.2020.128276

Tzani A, Vaitsis C, Kritsi E, Ivanov M, Soković M, Zoumpoulakis P, Detsi A. Green synthesis of bis-(β-dicarbonyl)-methane derivatives and biological evaluation as putative anticandidial agents. in Journal of Molecular Structure. 2020;1216:128276.
doi:10.1016/j.molstruc.2020.128276 .

Tzani, Andromachi, Vaitsis, Christos, Kritsi, Eftichia, Ivanov, Marija, Soković, Marina, Zoumpoulakis, Panagiotis, Detsi, Anastasia, "Green synthesis of bis-(β-dicarbonyl)-methane derivatives and biological evaluation as putative anticandidial agents" in Journal of Molecular Structure, 1216 (2020):128276,
https://doi.org/10.1016/j.molstruc.2020.128276 . .

TY  - JOUR
AU  - Kritsi, Eftichia
AU  - Matsoukas, Minos-Timotheos
AU  - Potamitis, Constantinos
AU  - Detsi, Anastasia
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Zoumpoulakis, Panagiotis
PY  - 2019
UR  - https://www.mdpi.com/1420-3049/24/21/3853
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3510
AB  - The prevalence of invasive fungal infections has been dramatically increased as the size of the immunocompromised population worldwide has grown. Aspergillus fumigatus is characterized as one of the most widespread and ubiquitous fungal pathogens. Among antifungal drugs, azoles have been the most widely used category for the treatment of fungal infections. However, increasingly, azole-resistant strains constitute a major problem to be faced. Towards this direction, our study focused on the identification of compounds bearing novel structural motifs which may evolve as a new class of antifungals. To fulfil this scope, a combination of in silico techniques and in vitro assays were implemented. Specifically, a ligand-based pharmacophore model was created and served as a 3D search query to screen the ZINC chemical database. Additionally, molecular docking and molecular dynamics simulations were used to improve the reliability and accuracy of virtual screening results. In total, eight compounds, bearing completely different chemical scaffolds from the commercially available azoles, were proposed and their antifungal activity was evaluated using in vitro assays. Results indicated that all tested compounds exhibit antifungal activity, especially compounds 1, 2, and 4, which presented the most promising minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values and, therefore, could be subjected to further hit to lead optimization.
T2  - Molecules
T1  - Novel Hit Compounds as Putative Antifungals: The Case of Aspergillus fumigatus
IS  - 21
VL  - 24
DO  - 10.3390/molecules24213853
SP  - 3853
ER  - 

@article{
author = "Kritsi, Eftichia and Matsoukas, Minos-Timotheos and Potamitis, Constantinos and Detsi, Anastasia and Ivanov, Marija and Soković, Marina and Zoumpoulakis, Panagiotis",
year = "2019",
abstract = "The prevalence of invasive fungal infections has been dramatically increased as the size of the immunocompromised population worldwide has grown. Aspergillus fumigatus is characterized as one of the most widespread and ubiquitous fungal pathogens. Among antifungal drugs, azoles have been the most widely used category for the treatment of fungal infections. However, increasingly, azole-resistant strains constitute a major problem to be faced. Towards this direction, our study focused on the identification of compounds bearing novel structural motifs which may evolve as a new class of antifungals. To fulfil this scope, a combination of in silico techniques and in vitro assays were implemented. Specifically, a ligand-based pharmacophore model was created and served as a 3D search query to screen the ZINC chemical database. Additionally, molecular docking and molecular dynamics simulations were used to improve the reliability and accuracy of virtual screening results. In total, eight compounds, bearing completely different chemical scaffolds from the commercially available azoles, were proposed and their antifungal activity was evaluated using in vitro assays. Results indicated that all tested compounds exhibit antifungal activity, especially compounds 1, 2, and 4, which presented the most promising minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values and, therefore, could be subjected to further hit to lead optimization.",
journal = "Molecules",
title = "Novel Hit Compounds as Putative Antifungals: The Case of Aspergillus fumigatus",
number = "21",
volume = "24",
doi = "10.3390/molecules24213853",
pages = "3853"
}

Kritsi, E., Matsoukas, M., Potamitis, C., Detsi, A., Ivanov, M., Soković, M.,& Zoumpoulakis, P.. (2019). Novel Hit Compounds as Putative Antifungals: The Case of Aspergillus fumigatus. in Molecules, 24(21), 3853.
https://doi.org/10.3390/molecules24213853

Kritsi E, Matsoukas M, Potamitis C, Detsi A, Ivanov M, Soković M, Zoumpoulakis P. Novel Hit Compounds as Putative Antifungals: The Case of Aspergillus fumigatus. in Molecules. 2019;24(21):3853.
doi:10.3390/molecules24213853 .

Kritsi, Eftichia, Matsoukas, Minos-Timotheos, Potamitis, Constantinos, Detsi, Anastasia, Ivanov, Marija, Soković, Marina, Zoumpoulakis, Panagiotis, "Novel Hit Compounds as Putative Antifungals: The Case of Aspergillus fumigatus" in Molecules, 24, no. 21 (2019):3853,
https://doi.org/10.3390/molecules24213853 . .

TY  - JOUR
AU  - Tratrat, Christophe
AU  - Haroun, Michelyne
AU  - Paparisva, Aliki
AU  - Geronikaki, Athina
AU  - Camoutsis, Charalabos
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Fotakis, Charalmpos
AU  - Zoumpoulakis, Panagiotis
AU  - Bhunia, Shome
AU  - Saxena, Anil
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4306
AB  - As a part of our ongoing studies in developing new derivatives as antimicrobial agents we
describe the synthesis of novel substituted 5-benzylideno-2-adamantylthiazol[3,2-b][1,2,4]triazol-6
(5H)ones.The twenty-five newly synthesized compounds were tested for their antimicrobial and
antifungal activity. All compounds have shown antibacterial properties with compounds 1–9 showing
the lowest activity, followed by compounds 10–14 while compounds 15–25 the highest antibacterial
activity. Specific compounds appeared to be more active than ampicillin in most studied
strains and in some cases more active than streptomycin. Antifungal activity in most cases also
was better than that of reference drugs ketoconazole and bifonazole. Elucidating the relation of molecular properties to antimicrobial activity as well as generation of pharmacophore model for
antifungal activity of two fungal species Aspergillus fumigatus and Candida albicans were performed.
PB  - Elsevier B.V. on behalf of King Saud University
T2  - Arabian Journal of Chemistry
T1  - Design, synthesis and biological evaluation of new substituted 5-benzylideno-2-adamantylthiazol[3,2- b][1,2,4]triazol-6(5H)ones. Pharmacophore models for antifungal activity
IS  - 4
VL  - 11
DO  - 10.1016/j.arabjc.2016.06.007
SP  - 573
EP  - 590
ER  - 

@article{
author = "Tratrat, Christophe and Haroun, Michelyne and Paparisva, Aliki and Geronikaki, Athina and Camoutsis, Charalabos and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina and Fotakis, Charalmpos and Zoumpoulakis, Panagiotis and Bhunia, Shome and Saxena, Anil",
year = "2018",
abstract = "As a part of our ongoing studies in developing new derivatives as antimicrobial agents we
describe the synthesis of novel substituted 5-benzylideno-2-adamantylthiazol[3,2-b][1,2,4]triazol-6
(5H)ones.The twenty-five newly synthesized compounds were tested for their antimicrobial and
antifungal activity. All compounds have shown antibacterial properties with compounds 1–9 showing
the lowest activity, followed by compounds 10–14 while compounds 15–25 the highest antibacterial
activity. Specific compounds appeared to be more active than ampicillin in most studied
strains and in some cases more active than streptomycin. Antifungal activity in most cases also
was better than that of reference drugs ketoconazole and bifonazole. Elucidating the relation of molecular properties to antimicrobial activity as well as generation of pharmacophore model for
antifungal activity of two fungal species Aspergillus fumigatus and Candida albicans were performed.",
publisher = "Elsevier B.V. on behalf of King Saud University",
journal = "Arabian Journal of Chemistry",
title = "Design, synthesis and biological evaluation of new substituted 5-benzylideno-2-adamantylthiazol[3,2- b][1,2,4]triazol-6(5H)ones. Pharmacophore models for antifungal activity",
number = "4",
volume = "11",
doi = "10.1016/j.arabjc.2016.06.007",
pages = "573-590"
}

Tratrat, C., Haroun, M., Paparisva, A., Geronikaki, A., Camoutsis, C., Ćirić, A., Glamočlija, J., Soković, M., Fotakis, C., Zoumpoulakis, P., Bhunia, S.,& Saxena, A.. (2018). Design, synthesis and biological evaluation of new substituted 5-benzylideno-2-adamantylthiazol[3,2- b][1,2,4]triazol-6(5H)ones. Pharmacophore models for antifungal activity. in Arabian Journal of Chemistry
Elsevier B.V. on behalf of King Saud University., 11(4), 573-590.
https://doi.org/10.1016/j.arabjc.2016.06.007

Tratrat C, Haroun M, Paparisva A, Geronikaki A, Camoutsis C, Ćirić A, Glamočlija J, Soković M, Fotakis C, Zoumpoulakis P, Bhunia S, Saxena A. Design, synthesis and biological evaluation of new substituted 5-benzylideno-2-adamantylthiazol[3,2- b][1,2,4]triazol-6(5H)ones. Pharmacophore models for antifungal activity. in Arabian Journal of Chemistry. 2018;11(4):573-590.
doi:10.1016/j.arabjc.2016.06.007 .

Tratrat, Christophe, Haroun, Michelyne, Paparisva, Aliki, Geronikaki, Athina, Camoutsis, Charalabos, Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, Fotakis, Charalmpos, Zoumpoulakis, Panagiotis, Bhunia, Shome, Saxena, Anil, "Design, synthesis and biological evaluation of new substituted 5-benzylideno-2-adamantylthiazol[3,2- b][1,2,4]triazol-6(5H)ones. Pharmacophore models for antifungal activity" in Arabian Journal of Chemistry, 11, no. 4 (2018):573-590,
https://doi.org/10.1016/j.arabjc.2016.06.007 . .

TY  - JOUR
AU  - Ivanov, Marija
AU  - Matsoukas, Minos-Timotheos
AU  - Kritsi, Eftichia
AU  - Zelenko, Urska
AU  - Golic Grdadolnik, Simona
AU  - Calhelha, Ricardo C.
AU  - Ferreira, Isabel C. F. R.
AU  - Sanković-Babić, Snežana
AU  - Glamočlija, Jasmina
AU  - Fotopoulou, Theano
AU  - Koufaki, Maria
AU  - Zoumpoulakis, Panagiotis
AU  - Soković, Marina
PY  - 2018
UR  - http://doi.wiley.com/10.1002/cmdc.201700602
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29235267
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2968
AB  - Four heteroaromatic compounds bearing nitrate esters were selected using a virtual-screening procedure as putative sterol 14α-demethylase (CYP51) Candida albicans inhibitors. Compounds were examined for their inhibition on C. albicans growth and biofilm formation as well as for their toxicity. NMR spectroscopy studies, in silico docking, and molecular dynamics simulations were used to investigate further the selectivity of these compounds to fungal CYP51. All compounds exhibited good antimicrobial properties, indicated with low minimal inhibitory concentrations and ability to inhibit formation of fungal biofilm. Moreover, all of the compounds had the ability to inhibit growth of C. albicans cells. N-(2-Nitrooxyethyl)-1Η-indole-2-carboxamide was the only compound with selectivity on C. albicans CYP51 that did not exhibit cytotoxic effect on cells isolated from liver and should be further investigated for selective application in new leads for the treatment of candidiasis.
T2  - ChemMedChem
T1  - Nitrate Esters of Heteroaromatic Compounds as Candida albicans CYP51 Enzyme Inhibitors.
IS  - 4
VL  - 32
DO  - 10.1002/cmdc.201700602
SP  - 342
EP  - 349
ER  - 

@article{
author = "Ivanov, Marija and Matsoukas, Minos-Timotheos and Kritsi, Eftichia and Zelenko, Urska and Golic Grdadolnik, Simona and Calhelha, Ricardo C. and Ferreira, Isabel C. F. R. and Sanković-Babić, Snežana and Glamočlija, Jasmina and Fotopoulou, Theano and Koufaki, Maria and Zoumpoulakis, Panagiotis and Soković, Marina",
year = "2018",
abstract = "Four heteroaromatic compounds bearing nitrate esters were selected using a virtual-screening procedure as putative sterol 14α-demethylase (CYP51) Candida albicans inhibitors. Compounds were examined for their inhibition on C. albicans growth and biofilm formation as well as for their toxicity. NMR spectroscopy studies, in silico docking, and molecular dynamics simulations were used to investigate further the selectivity of these compounds to fungal CYP51. All compounds exhibited good antimicrobial properties, indicated with low minimal inhibitory concentrations and ability to inhibit formation of fungal biofilm. Moreover, all of the compounds had the ability to inhibit growth of C. albicans cells. N-(2-Nitrooxyethyl)-1Η-indole-2-carboxamide was the only compound with selectivity on C. albicans CYP51 that did not exhibit cytotoxic effect on cells isolated from liver and should be further investigated for selective application in new leads for the treatment of candidiasis.",
journal = "ChemMedChem",
title = "Nitrate Esters of Heteroaromatic Compounds as Candida albicans CYP51 Enzyme Inhibitors.",
number = "4",
volume = "32",
doi = "10.1002/cmdc.201700602",
pages = "342-349"
}

Ivanov, M., Matsoukas, M., Kritsi, E., Zelenko, U., Golic Grdadolnik, S., Calhelha, R. C., Ferreira, I. C. F. R., Sanković-Babić, S., Glamočlija, J., Fotopoulou, T., Koufaki, M., Zoumpoulakis, P.,& Soković, M.. (2018). Nitrate Esters of Heteroaromatic Compounds as Candida albicans CYP51 Enzyme Inhibitors.. in ChemMedChem, 32(4), 342-349.
https://doi.org/10.1002/cmdc.201700602

Ivanov M, Matsoukas M, Kritsi E, Zelenko U, Golic Grdadolnik S, Calhelha RC, Ferreira ICFR, Sanković-Babić S, Glamočlija J, Fotopoulou T, Koufaki M, Zoumpoulakis P, Soković M. Nitrate Esters of Heteroaromatic Compounds as Candida albicans CYP51 Enzyme Inhibitors.. in ChemMedChem. 2018;32(4):342-349.
doi:10.1002/cmdc.201700602 .

Ivanov, Marija, Matsoukas, Minos-Timotheos, Kritsi, Eftichia, Zelenko, Urska, Golic Grdadolnik, Simona, Calhelha, Ricardo C., Ferreira, Isabel C. F. R., Sanković-Babić, Snežana, Glamočlija, Jasmina, Fotopoulou, Theano, Koufaki, Maria, Zoumpoulakis, Panagiotis, Soković, Marina, "Nitrate Esters of Heteroaromatic Compounds as Candida albicans CYP51 Enzyme Inhibitors." in ChemMedChem, 32, no. 4 (2018):342-349,
https://doi.org/10.1002/cmdc.201700602 . .

TY  - CONF
AU  - Petrović, Jovana
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Zoumpoulakis, Panagiotis
AU  - Proestos, Charalampos
AU  - Soković, Marina
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6376
AB  - Laetiporus sulphureus is an edible wood-rooting basidiomycete. The proximate composition, total phenol antioxidant 
capacity and antimicrobial activities of different extracts of L. sulphureus were determined. Different extraction 
methodologies, including high energy techniques, were employed and their effect was examined on the activity of the 
extracts. Optimum extraction methodologies (classical and ultrasound-assisted) provided one fraction containing 
neutral and polar lipids and the other fraction containing fungal carotenoids and pigments. Fatty acid analysis indicated 
a predominant level of polyunsaturated fatty acids followed by saturated and mono-unsaturated fatty acids. Both the 
aqueous methanolic and water extracts contained higher TPC and showed better antioxidant capacity than the ethanolic 
extract. Irrespective of the type of extraction applied, L. sulphureus showed good antimicrobial activity against all the 
tested bacteria and fungi, being in some cases stronger than the used antibiotics and mycotics. Therefore, this edible 
mushroom could be considered as a positive candidate to be utilised by the food industry, not only for obtaining 
bioactive compounds to be used as natural antioxidants/antimicrobial agents, but possibly also for its nutritional value 
and health benefits.
PB  - Belgrade: University of Belgrade
C3  - Unifood conference: Programme & Book of Abstracts; 2018 Oct 5-6; Belgrade, Serbia
T1  - Bioactive compounds of the wild edible mushroom Laetiporus sulphureus (Bull.) Murrill.  Antioxidant, antifungal and antibacterial properties
SP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6376
ER  - 

@conference{
author = "Petrović, Jovana and Glamočlija, Jasmina and Ćirić, Ana and Zoumpoulakis, Panagiotis and Proestos, Charalampos and Soković, Marina",
year = "2018",
abstract = "Laetiporus sulphureus is an edible wood-rooting basidiomycete. The proximate composition, total phenol antioxidant 
capacity and antimicrobial activities of different extracts of L. sulphureus were determined. Different extraction 
methodologies, including high energy techniques, were employed and their effect was examined on the activity of the 
extracts. Optimum extraction methodologies (classical and ultrasound-assisted) provided one fraction containing 
neutral and polar lipids and the other fraction containing fungal carotenoids and pigments. Fatty acid analysis indicated 
a predominant level of polyunsaturated fatty acids followed by saturated and mono-unsaturated fatty acids. Both the 
aqueous methanolic and water extracts contained higher TPC and showed better antioxidant capacity than the ethanolic 
extract. Irrespective of the type of extraction applied, L. sulphureus showed good antimicrobial activity against all the 
tested bacteria and fungi, being in some cases stronger than the used antibiotics and mycotics. Therefore, this edible 
mushroom could be considered as a positive candidate to be utilised by the food industry, not only for obtaining 
bioactive compounds to be used as natural antioxidants/antimicrobial agents, but possibly also for its nutritional value 
and health benefits.",
publisher = "Belgrade: University of Belgrade",
journal = "Unifood conference: Programme & Book of Abstracts; 2018 Oct 5-6; Belgrade, Serbia",
title = "Bioactive compounds of the wild edible mushroom Laetiporus sulphureus (Bull.) Murrill.  Antioxidant, antifungal and antibacterial properties",
pages = "14",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6376"
}

Petrović, J., Glamočlija, J., Ćirić, A., Zoumpoulakis, P., Proestos, C.,& Soković, M.. (2018). Bioactive compounds of the wild edible mushroom Laetiporus sulphureus (Bull.) Murrill.  Antioxidant, antifungal and antibacterial properties. in Unifood conference: Programme & Book of Abstracts; 2018 Oct 5-6; Belgrade, Serbia
Belgrade: University of Belgrade., 14.
https://hdl.handle.net/21.15107/rcub_ibiss_6376

Petrović J, Glamočlija J, Ćirić A, Zoumpoulakis P, Proestos C, Soković M. Bioactive compounds of the wild edible mushroom Laetiporus sulphureus (Bull.) Murrill.  Antioxidant, antifungal and antibacterial properties. in Unifood conference: Programme & Book of Abstracts; 2018 Oct 5-6; Belgrade, Serbia. 2018;:14.
https://hdl.handle.net/21.15107/rcub_ibiss_6376 .

Petrović, Jovana, Glamočlija, Jasmina, Ćirić, Ana, Zoumpoulakis, Panagiotis, Proestos, Charalampos, Soković, Marina, "Bioactive compounds of the wild edible mushroom Laetiporus sulphureus (Bull.) Murrill.  Antioxidant, antifungal and antibacterial properties" in Unifood conference: Programme & Book of Abstracts; 2018 Oct 5-6; Belgrade, Serbia (2018):14,
https://hdl.handle.net/21.15107/rcub_ibiss_6376 .

TY  - JOUR
AU  - Incerti, Matteo
AU  - Vicini, Paola
AU  - Geronikaki, Athina
AU  - Eleftheriou, Phaedra
AU  - Tsagkadouras, Athanasios
AU  - Zoumpoulakis, Panagiotis
AU  - Fotakis, Charalmpos
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2017
UR  - http://xlink.rsc.org/?DOI=C7MD00334J
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2926
AB  - A series of 21 novel N-[2-phenyl-4-oxo-1,3-thiazolidin-3-yl]-1,2-benzothiazole-3-carboxamides/acetamides (4a–4p) as well as a series of N′-(halophenylmethylidene)-1,2-benzothiazole-3-acetohydrazides (3h–3p) have been synthesized and evaluated for their antimicrobial activity against eight bacterial and eight fungal species, among them plant, animal and human pathogens and food contaminating species. All compounds appeared to be potent and the best activity was exhibited by compound 4d with MIC in the range of 10.7–21.4 μmol mL−1 × 10−2 and MBC of 21.4–40.2 μmol mL−1 × 10−2. The best antifungal activity was observed for compounds 4p and 3h. Elucidation of the relationship between the antimicrobial activity and molecular properties of the synthesized compounds was also performed. Synthetic intermediates were also tested with several exhibiting good antimicrobial activities. Docking studies for some compounds were performed.
T2  - MedChemComm
T2  - MedChemComm
T1  - New N -(2-phenyl-4-oxo-1,3-thiazolidin-3-yl)-1,2-benzothiazole-3-carboxamides and acetamides as antimicrobial agents
IS  - 11
VL  - 8
DO  - 10.1039/C7MD00334J
SP  - 2142
EP  - 2154
ER  - 

@article{
author = "Incerti, Matteo and Vicini, Paola and Geronikaki, Athina and Eleftheriou, Phaedra and Tsagkadouras, Athanasios and Zoumpoulakis, Panagiotis and Fotakis, Charalmpos and Ćirić, Ana and Glamočlija, Jasmina and Soković, Marina",
year = "2017",
abstract = "A series of 21 novel N-[2-phenyl-4-oxo-1,3-thiazolidin-3-yl]-1,2-benzothiazole-3-carboxamides/acetamides (4a–4p) as well as a series of N′-(halophenylmethylidene)-1,2-benzothiazole-3-acetohydrazides (3h–3p) have been synthesized and evaluated for their antimicrobial activity against eight bacterial and eight fungal species, among them plant, animal and human pathogens and food contaminating species. All compounds appeared to be potent and the best activity was exhibited by compound 4d with MIC in the range of 10.7–21.4 μmol mL−1 × 10−2 and MBC of 21.4–40.2 μmol mL−1 × 10−2. The best antifungal activity was observed for compounds 4p and 3h. Elucidation of the relationship between the antimicrobial activity and molecular properties of the synthesized compounds was also performed. Synthetic intermediates were also tested with several exhibiting good antimicrobial activities. Docking studies for some compounds were performed.",
journal = "MedChemComm, MedChemComm",
title = "New N -(2-phenyl-4-oxo-1,3-thiazolidin-3-yl)-1,2-benzothiazole-3-carboxamides and acetamides as antimicrobial agents",
number = "11",
volume = "8",
doi = "10.1039/C7MD00334J",
pages = "2142-2154"
}

Incerti, M., Vicini, P., Geronikaki, A., Eleftheriou, P., Tsagkadouras, A., Zoumpoulakis, P., Fotakis, C., Ćirić, A., Glamočlija, J.,& Soković, M.. (2017). New N -(2-phenyl-4-oxo-1,3-thiazolidin-3-yl)-1,2-benzothiazole-3-carboxamides and acetamides as antimicrobial agents. in MedChemComm, 8(11), 2142-2154.
https://doi.org/10.1039/C7MD00334J

Incerti M, Vicini P, Geronikaki A, Eleftheriou P, Tsagkadouras A, Zoumpoulakis P, Fotakis C, Ćirić A, Glamočlija J, Soković M. New N -(2-phenyl-4-oxo-1,3-thiazolidin-3-yl)-1,2-benzothiazole-3-carboxamides and acetamides as antimicrobial agents. in MedChemComm. 2017;8(11):2142-2154.
doi:10.1039/C7MD00334J .

Incerti, Matteo, Vicini, Paola, Geronikaki, Athina, Eleftheriou, Phaedra, Tsagkadouras, Athanasios, Zoumpoulakis, Panagiotis, Fotakis, Charalmpos, Ćirić, Ana, Glamočlija, Jasmina, Soković, Marina, "New N -(2-phenyl-4-oxo-1,3-thiazolidin-3-yl)-1,2-benzothiazole-3-carboxamides and acetamides as antimicrobial agents" in MedChemComm, 8, no. 11 (2017):2142-2154,
https://doi.org/10.1039/C7MD00334J . .

TY  - JOUR
AU  - Fotopoulou, Theano
AU  - Ćirić, Ana
AU  - Kritsi, Eftichia
AU  - Calhelha, Ricardo C.
AU  - Ferreira, Isabel C.F.R.
AU  - Soković, Marina
AU  - Zoumpoulakis, Panagiotis
AU  - Koufaki, Maria
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6747
AB  - Natural b-thujaplicin displays a remarkable array of biological activities for the prevention or
treatment of various disorders while its tropolone scaffold inspired the synthesis of new analogs. The
main goal of the current study was to evaluate the influence of 4-substituted piperazine moieties at
position 7 of the b-thujaplicin scaffold, on the antimicrobial activity. In order to determine the
biological activity of the b-thujaplicin derivatives, a microdilution method was used against a wide
variety of bacteria and fungi. Pseudomonas aeruginosa PAO 1 was used for testing antiquorum and
antibiofilm effects. Four human tumor cell lines (MCF-7, NCI-H460, HeLa, and HepG2) and a porcine
liver derived cell line (PLP2) were used for testing antitumor and cytotoxic activity. The compounds
present better antibacterial and antifungal activity in comparison with approved antimicrobials used
as control agents. b-Thujaplicin showed strong antibacterial and antifungal activities against all
tested species. Further studies of their antibacterial activity revealed that all compounds presented
good antibiofilm and antiquorum effects. Fungi were more susceptible than bacteria to the tested
compounds, with the exception of MK150, which possessed the best antibacterial effect. None of the
tested compounds, at the GI50 values obtained for the tumor cell lines, have shown toxicity for non tumor liver cells (PLP2). The prediction of physicochemical properties of the compounds was
performed to further explain the structure–activity relationship. Finally, in order to explore a possible
mechanism of action of the synthesized compounds, molecular docking studies were performed on
CYP51 (14-a lanosterol demethylase), an important component of the fungal cell membrane.
PB  - Weinheim, Germany: WILEY-VCH Verlag GmbH & Co. KGaA,
T2  - Archiv der Pharmazie
T1  - Antimicrobial/Antibiofilm Activity and Cytotoxic Studies of b-Thujaplicin Derivatives
IS  - 9
VL  - 349
DO  - 10.1002/ardp.201600095
SP  - 698
EP  - 709
ER  - 

@article{
author = "Fotopoulou, Theano and Ćirić, Ana and Kritsi, Eftichia and Calhelha, Ricardo C. and Ferreira, Isabel C.F.R. and Soković, Marina and Zoumpoulakis, Panagiotis and Koufaki, Maria",
year = "2016",
abstract = "Natural b-thujaplicin displays a remarkable array of biological activities for the prevention or
treatment of various disorders while its tropolone scaffold inspired the synthesis of new analogs. The
main goal of the current study was to evaluate the influence of 4-substituted piperazine moieties at
position 7 of the b-thujaplicin scaffold, on the antimicrobial activity. In order to determine the
biological activity of the b-thujaplicin derivatives, a microdilution method was used against a wide
variety of bacteria and fungi. Pseudomonas aeruginosa PAO 1 was used for testing antiquorum and
antibiofilm effects. Four human tumor cell lines (MCF-7, NCI-H460, HeLa, and HepG2) and a porcine
liver derived cell line (PLP2) were used for testing antitumor and cytotoxic activity. The compounds
present better antibacterial and antifungal activity in comparison with approved antimicrobials used
as control agents. b-Thujaplicin showed strong antibacterial and antifungal activities against all
tested species. Further studies of their antibacterial activity revealed that all compounds presented
good antibiofilm and antiquorum effects. Fungi were more susceptible than bacteria to the tested
compounds, with the exception of MK150, which possessed the best antibacterial effect. None of the
tested compounds, at the GI50 values obtained for the tumor cell lines, have shown toxicity for non tumor liver cells (PLP2). The prediction of physicochemical properties of the compounds was
performed to further explain the structure–activity relationship. Finally, in order to explore a possible
mechanism of action of the synthesized compounds, molecular docking studies were performed on
CYP51 (14-a lanosterol demethylase), an important component of the fungal cell membrane.",
publisher = "Weinheim, Germany: WILEY-VCH Verlag GmbH & Co. KGaA,",
journal = "Archiv der Pharmazie",
title = "Antimicrobial/Antibiofilm Activity and Cytotoxic Studies of b-Thujaplicin Derivatives",
number = "9",
volume = "349",
doi = "10.1002/ardp.201600095",
pages = "698-709"
}

Fotopoulou, T., Ćirić, A., Kritsi, E., Calhelha, R. C., Ferreira, I. C.F.R., Soković, M., Zoumpoulakis, P.,& Koufaki, M.. (2016). Antimicrobial/Antibiofilm Activity and Cytotoxic Studies of b-Thujaplicin Derivatives. in Archiv der Pharmazie
Weinheim, Germany: WILEY-VCH Verlag GmbH & Co. KGaA,., 349(9), 698-709.
https://doi.org/10.1002/ardp.201600095

Fotopoulou T, Ćirić A, Kritsi E, Calhelha RC, Ferreira IC, Soković M, Zoumpoulakis P, Koufaki M. Antimicrobial/Antibiofilm Activity and Cytotoxic Studies of b-Thujaplicin Derivatives. in Archiv der Pharmazie. 2016;349(9):698-709.
doi:10.1002/ardp.201600095 .

Fotopoulou, Theano, Ćirić, Ana, Kritsi, Eftichia, Calhelha, Ricardo C., Ferreira, Isabel C.F.R., Soković, Marina, Zoumpoulakis, Panagiotis, Koufaki, Maria, "Antimicrobial/Antibiofilm Activity and Cytotoxic Studies of b-Thujaplicin Derivatives" in Archiv der Pharmazie, 349, no. 9 (2016):698-709,
https://doi.org/10.1002/ardp.201600095 . .

TY  - JOUR
AU  - Sinanoglou, Vassilia
AU  - Zoumpoulakis, Panagiotis
AU  - Heropoulos, George
AU  - Proestos, Charalampos
AU  - Ćirić, Ana
AU  - Petrović, Jovana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6415
AB  - Laetiporus sulphureus is a saprophyte belonging to
a specific group of wood-decomposing Basidiomycetes grow-
ing on deciduous trees. This fungus has been characterized as
a herbal medicine and is also known for its antimicrobial
properties. In the present study, high energy extraction tech-
niques using different solvents were compared to obtain max-
imum yield of the edible fungus Laetiporus sulphureus total
lipids. The lipid classes and fatty acid composition of the
fruiting bodies’ total lipids has been studied using GC-FID
and Iatroscan TLC-FID analysis. Among the lipids, the neu-
tral lipids predominated followed by phospholipids and gly-
colipids. Triglycerides were the most abundant in the neutral
lipid fraction, whereas phosphatidylcholine in phospholipids.
The existence of relatively high amount of sterols may be
correlated to fungus pharmaceutical properties. Total lipids
were found to contain high unsaturated degree fatty acids
(UFA/SFA>3.4) and dominated of C18:2ω-6, C18:1ω-9
and C16:0 fatty acids. Antibacterial and antifungal properties
of mushrooms’ lipid extracts from two different solvents were
also examined. Results indicated that hexane extracts pos-
sessed better antifungal and slightly better antibacterial activ-
ity compared to chloroform extracts though both were less
active than the commercial antimicrobial agents.
PB  - Springer
T2  - Journal of Food Science and Technology
T1  - Lipid and fatty acid profile of the edible fungus Laetiporus sulphurous. Antifungal and antibacterial properties
IS  - 6
VL  - 52
DO  - 10.1007/s13197-014-1377-8
SP  - 3264
EP  - 3272
ER  - 

@article{
author = "Sinanoglou, Vassilia and Zoumpoulakis, Panagiotis and Heropoulos, George and Proestos, Charalampos and Ćirić, Ana and Petrović, Jovana and Glamočlija, Jasmina and Soković, Marina",
year = "2015",
abstract = "Laetiporus sulphureus is a saprophyte belonging to
a specific group of wood-decomposing Basidiomycetes grow-
ing on deciduous trees. This fungus has been characterized as
a herbal medicine and is also known for its antimicrobial
properties. In the present study, high energy extraction tech-
niques using different solvents were compared to obtain max-
imum yield of the edible fungus Laetiporus sulphureus total
lipids. The lipid classes and fatty acid composition of the
fruiting bodies’ total lipids has been studied using GC-FID
and Iatroscan TLC-FID analysis. Among the lipids, the neu-
tral lipids predominated followed by phospholipids and gly-
colipids. Triglycerides were the most abundant in the neutral
lipid fraction, whereas phosphatidylcholine in phospholipids.
The existence of relatively high amount of sterols may be
correlated to fungus pharmaceutical properties. Total lipids
were found to contain high unsaturated degree fatty acids
(UFA/SFA>3.4) and dominated of C18:2ω-6, C18:1ω-9
and C16:0 fatty acids. Antibacterial and antifungal properties
of mushrooms’ lipid extracts from two different solvents were
also examined. Results indicated that hexane extracts pos-
sessed better antifungal and slightly better antibacterial activ-
ity compared to chloroform extracts though both were less
active than the commercial antimicrobial agents.",
publisher = "Springer",
journal = "Journal of Food Science and Technology",
title = "Lipid and fatty acid profile of the edible fungus Laetiporus sulphurous. Antifungal and antibacterial properties",
number = "6",
volume = "52",
doi = "10.1007/s13197-014-1377-8",
pages = "3264-3272"
}

Sinanoglou, V., Zoumpoulakis, P., Heropoulos, G., Proestos, C., Ćirić, A., Petrović, J., Glamočlija, J.,& Soković, M.. (2015). Lipid and fatty acid profile of the edible fungus Laetiporus sulphurous. Antifungal and antibacterial properties. in Journal of Food Science and Technology
Springer., 52(6), 3264-3272.
https://doi.org/10.1007/s13197-014-1377-8

Sinanoglou V, Zoumpoulakis P, Heropoulos G, Proestos C, Ćirić A, Petrović J, Glamočlija J, Soković M. Lipid and fatty acid profile of the edible fungus Laetiporus sulphurous. Antifungal and antibacterial properties. in Journal of Food Science and Technology. 2015;52(6):3264-3272.
doi:10.1007/s13197-014-1377-8 .

Sinanoglou, Vassilia, Zoumpoulakis, Panagiotis, Heropoulos, George, Proestos, Charalampos, Ćirić, Ana, Petrović, Jovana, Glamočlija, Jasmina, Soković, Marina, "Lipid and fatty acid profile of the edible fungus Laetiporus sulphurous. Antifungal and antibacterial properties" in Journal of Food Science and Technology, 52, no. 6 (2015):3264-3272,
https://doi.org/10.1007/s13197-014-1377-8 . .

TY  - JOUR
AU  - Petrović, Jovana
AU  - Papandreou, Magdalini
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Baskakis, Constantinos
AU  - Proestos, Charalampos
AU  - Lamari, Fotini
AU  - Zoumpoulakis, Panagiotis
AU  - Soković, Marina
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2132
AB  - Laetiporus sulphureus is an edible wood-rooting basidiomycete. The
   nutritional and medicinal properties of this mushroom have long been
   known by traditional practitioners. The aim of this study was to
   determine the proximate composition, total phenol antioxidant capacity
   and antimicrobial activities of different extracts of L. sulphureus.
   Different extraction methodologies, including high energy techniques,
   were employed and their effect was examined on the activity of the
   extracts. Optimum extraction methodologies (classical and
   ultrasound-assisted) provided one fraction containing neutral and polar
   lipids and the other fraction containing fungal carotenoids and
   pigments. Fatty acid analysis indicated a predominant level of
   polyunsaturated fatty acids followed by saturated and mono-unsaturated
   fatty acids. Both the aqueous methanolic and water extracts contained
   higher TPC and showed better antioxidant capacity than the ethanolic
   extract. Irrespective of the type of extraction applied, L. sulphureus
   showed good antimicrobial activity against all the tested bacteria and
   fungi, being in some cases stronger than the used antibiotics and
   mycotics. Therefore, this edible mushroom could be considered as a
   positive candidate to be utilised by the food industry, not only for
   obtaining bioactive compounds to be used as natural
   antioxidants/antimicrobial agents, but possibly also for its nutritional
   value and health benefits.
T2  - Food & Function
T1  - Different extraction methodologies and their influence on the
 bioactivity of the wild edible mushroom Laetiporus sulphureus (Bull.)
 Murrill
IS  - 11
VL  - 5
DO  - 10.1039/c4fo00727a
SP  - 2948
EP  - 2960
ER  - 

@article{
author = "Petrović, Jovana and Papandreou, Magdalini and Glamočlija, Jasmina and Ćirić, Ana and Baskakis, Constantinos and Proestos, Charalampos and Lamari, Fotini and Zoumpoulakis, Panagiotis and Soković, Marina",
year = "2014",
abstract = "Laetiporus sulphureus is an edible wood-rooting basidiomycete. The
   nutritional and medicinal properties of this mushroom have long been
   known by traditional practitioners. The aim of this study was to
   determine the proximate composition, total phenol antioxidant capacity
   and antimicrobial activities of different extracts of L. sulphureus.
   Different extraction methodologies, including high energy techniques,
   were employed and their effect was examined on the activity of the
   extracts. Optimum extraction methodologies (classical and
   ultrasound-assisted) provided one fraction containing neutral and polar
   lipids and the other fraction containing fungal carotenoids and
   pigments. Fatty acid analysis indicated a predominant level of
   polyunsaturated fatty acids followed by saturated and mono-unsaturated
   fatty acids. Both the aqueous methanolic and water extracts contained
   higher TPC and showed better antioxidant capacity than the ethanolic
   extract. Irrespective of the type of extraction applied, L. sulphureus
   showed good antimicrobial activity against all the tested bacteria and
   fungi, being in some cases stronger than the used antibiotics and
   mycotics. Therefore, this edible mushroom could be considered as a
   positive candidate to be utilised by the food industry, not only for
   obtaining bioactive compounds to be used as natural
   antioxidants/antimicrobial agents, but possibly also for its nutritional
   value and health benefits.",
journal = "Food & Function",
title = "Different extraction methodologies and their influence on the
 bioactivity of the wild edible mushroom Laetiporus sulphureus (Bull.)
 Murrill",
number = "11",
volume = "5",
doi = "10.1039/c4fo00727a",
pages = "2948-2960"
}

Petrović, J., Papandreou, M., Glamočlija, J., Ćirić, A., Baskakis, C., Proestos, C., Lamari, F., Zoumpoulakis, P.,& Soković, M.. (2014). Different extraction methodologies and their influence on the
 bioactivity of the wild edible mushroom Laetiporus sulphureus (Bull.)
 Murrill. in Food & Function, 5(11), 2948-2960.
https://doi.org/10.1039/c4fo00727a

Petrović J, Papandreou M, Glamočlija J, Ćirić A, Baskakis C, Proestos C, Lamari F, Zoumpoulakis P, Soković M. Different extraction methodologies and their influence on the
 bioactivity of the wild edible mushroom Laetiporus sulphureus (Bull.)
 Murrill. in Food & Function. 2014;5(11):2948-2960.
doi:10.1039/c4fo00727a .

Petrović, Jovana, Papandreou, Magdalini, Glamočlija, Jasmina, Ćirić, Ana, Baskakis, Constantinos, Proestos, Charalampos, Lamari, Fotini, Zoumpoulakis, Panagiotis, Soković, Marina, "Different extraction methodologies and their influence on the
 bioactivity of the wild edible mushroom Laetiporus sulphureus (Bull.)
 Murrill" in Food & Function, 5, no. 11 (2014):2948-2960,
https://doi.org/10.1039/c4fo00727a . .

TY  - JOUR
AU  - Omar, Kouatli
AU  - Geronikaki, Athina
AU  - Zoumpoulakis, Panagiotis
AU  - Camoutsis, Charalabos
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Glamočlija, Jasmina
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3588
AB  - As part of ongoing studies in developing new antimicrobials, a class of structurally novel 4-thiazolidinone derivatives incorporating three known bioactive nuclei such as thiazole, thiazolidinone and adamantane was synthesized by the multi-step reaction protocol, already reported in the literature. NMR and Molecular Modeling techniques were employed for structure elucidation and Z/E potential isomerism configuration of the analogues. Evaluation of antibacterial and antifungal activity showed that almost all compounds exhibited better results than reference drugs thus they could be promising candidates for novel drugs.
PB  - Elsevier BV
T2  - Bioorganic & Medicinal Chemistry
T1  - Novel 4-thiazolidinone derivatives as potential antifungal and antibacterial drugs
IS  - 1
VL  - 18
DO  - 10.1016/j.bmc.2009.10.041
SP  - 426
EP  - 432
ER  - 

@article{
author = "Omar, Kouatli and Geronikaki, Athina and Zoumpoulakis, Panagiotis and Camoutsis, Charalabos and Soković, Marina and Ćirić, Ana and Glamočlija, Jasmina",
year = "2010",
abstract = "As part of ongoing studies in developing new antimicrobials, a class of structurally novel 4-thiazolidinone derivatives incorporating three known bioactive nuclei such as thiazole, thiazolidinone and adamantane was synthesized by the multi-step reaction protocol, already reported in the literature. NMR and Molecular Modeling techniques were employed for structure elucidation and Z/E potential isomerism configuration of the analogues. Evaluation of antibacterial and antifungal activity showed that almost all compounds exhibited better results than reference drugs thus they could be promising candidates for novel drugs.",
publisher = "Elsevier BV",
journal = "Bioorganic & Medicinal Chemistry",
title = "Novel 4-thiazolidinone derivatives as potential antifungal and antibacterial drugs",
number = "1",
volume = "18",
doi = "10.1016/j.bmc.2009.10.041",
pages = "426-432"
}

Omar, K., Geronikaki, A., Zoumpoulakis, P., Camoutsis, C., Soković, M., Ćirić, A.,& Glamočlija, J.. (2010). Novel 4-thiazolidinone derivatives as potential antifungal and antibacterial drugs. in Bioorganic & Medicinal Chemistry
Elsevier BV., 18(1), 426-432.
https://doi.org/10.1016/j.bmc.2009.10.041

Omar K, Geronikaki A, Zoumpoulakis P, Camoutsis C, Soković M, Ćirić A, Glamočlija J. Novel 4-thiazolidinone derivatives as potential antifungal and antibacterial drugs. in Bioorganic & Medicinal Chemistry. 2010;18(1):426-432.
doi:10.1016/j.bmc.2009.10.041 .

Omar, Kouatli, Geronikaki, Athina, Zoumpoulakis, Panagiotis, Camoutsis, Charalabos, Soković, Marina, Ćirić, Ana, Glamočlija, Jasmina, "Novel 4-thiazolidinone derivatives as potential antifungal and antibacterial drugs" in Bioorganic & Medicinal Chemistry, 18, no. 1 (2010):426-432,
https://doi.org/10.1016/j.bmc.2009.10.041 . .

TY  - JOUR
AU  - Ezabadi, Iraj Rahavi
AU  - Camoutsis, Charalabos
AU  - Zoumpoulakis, Panagiotis
AU  - Geronikaki, Athina
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3586
AB  - A series of 10 new 5-[2-(substituted sulfamoyl)-4,5-dimethoxy-benzyl]-4aryl-s-triazole-3-thiones were synthesized and evaluated for in vitro antifungal and antibacterial activity. All compounds tested showed significant antifungal activity against all the micromycetes, compared to the commercial fungicide bifonazole. Differences in their activity depend on the substitution of different reactive groups. More specifically, best antifungal activity among synthetic analogues was shown with N-dimethylsulfamoyl group. All the compounds tested against bacteria showed the same activity as the commercial agent streptomycin, except for Enterobacter cloacce and Salmonella species. Chloramphenicol showed lower bactericidal effect than the synthetic compounds. Furthermore, it is apparent that different compounds reacted in different ways against bacteria. Gram (-) bacteria seem to be more sensitive to these compounds than Gram (+) species. An effort was made to correlate the above-mentioned differences in activity with lipophilicity studies. Furthermore, molecular modeling was used to obtain the main conformational features of this class of molecules for future structure-activity relationship studies.
PB  - Elsevier BV
T2  - Bioorganic & Medicinal Chemistry
T1  - Sulfonamide-1,2,4-triazole derivatives as antifungal and antibacterial agents: Synthesis, biological evaluation, lipophilicity, and conformational studies
IS  - 3
VL  - 16
DO  - 10.1016/j.bmc.2007.10.082
SP  - 1150
EP  - 1161
ER  - 

@article{
author = "Ezabadi, Iraj Rahavi and Camoutsis, Charalabos and Zoumpoulakis, Panagiotis and Geronikaki, Athina and Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana",
year = "2008",
abstract = "A series of 10 new 5-[2-(substituted sulfamoyl)-4,5-dimethoxy-benzyl]-4aryl-s-triazole-3-thiones were synthesized and evaluated for in vitro antifungal and antibacterial activity. All compounds tested showed significant antifungal activity against all the micromycetes, compared to the commercial fungicide bifonazole. Differences in their activity depend on the substitution of different reactive groups. More specifically, best antifungal activity among synthetic analogues was shown with N-dimethylsulfamoyl group. All the compounds tested against bacteria showed the same activity as the commercial agent streptomycin, except for Enterobacter cloacce and Salmonella species. Chloramphenicol showed lower bactericidal effect than the synthetic compounds. Furthermore, it is apparent that different compounds reacted in different ways against bacteria. Gram (-) bacteria seem to be more sensitive to these compounds than Gram (+) species. An effort was made to correlate the above-mentioned differences in activity with lipophilicity studies. Furthermore, molecular modeling was used to obtain the main conformational features of this class of molecules for future structure-activity relationship studies.",
publisher = "Elsevier BV",
journal = "Bioorganic & Medicinal Chemistry",
title = "Sulfonamide-1,2,4-triazole derivatives as antifungal and antibacterial agents: Synthesis, biological evaluation, lipophilicity, and conformational studies",
number = "3",
volume = "16",
doi = "10.1016/j.bmc.2007.10.082",
pages = "1150-1161"
}

Ezabadi, I. R., Camoutsis, C., Zoumpoulakis, P., Geronikaki, A., Soković, M., Glamočlija, J.,& Ćirić, A.. (2008). Sulfonamide-1,2,4-triazole derivatives as antifungal and antibacterial agents: Synthesis, biological evaluation, lipophilicity, and conformational studies. in Bioorganic & Medicinal Chemistry
Elsevier BV., 16(3), 1150-1161.
https://doi.org/10.1016/j.bmc.2007.10.082

Ezabadi IR, Camoutsis C, Zoumpoulakis P, Geronikaki A, Soković M, Glamočlija J, Ćirić A. Sulfonamide-1,2,4-triazole derivatives as antifungal and antibacterial agents: Synthesis, biological evaluation, lipophilicity, and conformational studies. in Bioorganic & Medicinal Chemistry. 2008;16(3):1150-1161.
doi:10.1016/j.bmc.2007.10.082 .

Ezabadi, Iraj Rahavi, Camoutsis, Charalabos, Zoumpoulakis, Panagiotis, Geronikaki, Athina, Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, "Sulfonamide-1,2,4-triazole derivatives as antifungal and antibacterial agents: Synthesis, biological evaluation, lipophilicity, and conformational studies" in Bioorganic & Medicinal Chemistry, 16, no. 3 (2008):1150-1161,
https://doi.org/10.1016/j.bmc.2007.10.082 . .