TY  - CONF
AU  - Savić, Danijela
AU  - Lavrnja, Irena
AU  - Peković, Sanja
AU  - Šubašić, Sanja A
AU  - Jovanović, Sasa
AU  - Nikić, Ivana
AU  - Bjelobaba, Ivana
AU  - Mostarica-Stojković, Marija B
AU  - Stošić-Grujičić, Stanislava
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1636
AB  - Experimental autoimmune encephalomyelitis (EAE) is an animal model
of human disease multiple sclerosis (MS). Clinical signs of EAE are result of
an autoaggressive T-cell response against myelin. We have previously
shown that combined treatment with nucleoside analogues (ribavirin — R
+tiazofurin — T), inosine monophosphate dehydrogenase inhibitors,
ameliorates clinical signs and histological lesions of EAE in susceptible
rats, when they are given preventatively. The aim of this study was to
investigate the effect of combined treatment with R+T, given with the
appearance of first EAE clinical sign, on microglia and astrocytes response.
These cells of the target tissue also participate in an autoimmune process.
The disease was induced in Dark Agouti rats with rat spinal cord
homogenate and had acute monophasic course. Ribavirin and tiazofurin
were given at a dosage of 30 mg/kg/day and 10 mg/kg every other day, for
15 days, respectively. Control group was immunized and treated with saline.
Amelioration of clinical signs and faster recovery was shown in group
treated with combination of R and T in comparison to control group.
Immunohistochemical analysis of the spinal cord tissue isolated after
15 days of combined therapy revealed decrease in vimentin positive cells
and microglia compared to control group. Additionally, morphology of
GFAP positive (glial fibrillary acid protein) cells and microglia indicated to
reactive type of these cells in control group. Results of this study revealed that R and T modulate glial response and have EAE protective effects when
they are given from the onset of disease.
C3  - 8th ISNI Congress; 2006 Oct 15-19; Nagoya, Japan
T1  - Nucleoside analogues effect on glial response in experimental autoimmune encephalomyelitis
SP  - 167
EP  - 168
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1636
ER  - 

@conference{
author = "Savić, Danijela and Lavrnja, Irena and Peković, Sanja and Šubašić, Sanja A and Jovanović, Sasa and Nikić, Ivana and Bjelobaba, Ivana and Mostarica-Stojković, Marija B and Stošić-Grujičić, Stanislava and Rakić, Ljubisav and Stojiljković, Mirjana B",
year = "2006",
abstract = "Experimental autoimmune encephalomyelitis (EAE) is an animal model
of human disease multiple sclerosis (MS). Clinical signs of EAE are result of
an autoaggressive T-cell response against myelin. We have previously
shown that combined treatment with nucleoside analogues (ribavirin — R
+tiazofurin — T), inosine monophosphate dehydrogenase inhibitors,
ameliorates clinical signs and histological lesions of EAE in susceptible
rats, when they are given preventatively. The aim of this study was to
investigate the effect of combined treatment with R+T, given with the
appearance of first EAE clinical sign, on microglia and astrocytes response.
These cells of the target tissue also participate in an autoimmune process.
The disease was induced in Dark Agouti rats with rat spinal cord
homogenate and had acute monophasic course. Ribavirin and tiazofurin
were given at a dosage of 30 mg/kg/day and 10 mg/kg every other day, for
15 days, respectively. Control group was immunized and treated with saline.
Amelioration of clinical signs and faster recovery was shown in group
treated with combination of R and T in comparison to control group.
Immunohistochemical analysis of the spinal cord tissue isolated after
15 days of combined therapy revealed decrease in vimentin positive cells
and microglia compared to control group. Additionally, morphology of
GFAP positive (glial fibrillary acid protein) cells and microglia indicated to
reactive type of these cells in control group. Results of this study revealed that R and T modulate glial response and have EAE protective effects when
they are given from the onset of disease.",
journal = "8th ISNI Congress; 2006 Oct 15-19; Nagoya, Japan",
title = "Nucleoside analogues effect on glial response in experimental autoimmune encephalomyelitis",
pages = "167-168",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1636"
}

Savić, D., Lavrnja, I., Peković, S., Šubašić, S. A., Jovanović, S., Nikić, I., Bjelobaba, I., Mostarica-Stojković, M. B., Stošić-Grujičić, S., Rakić, L.,& Stojiljković, M. B.. (2006). Nucleoside analogues effect on glial response in experimental autoimmune encephalomyelitis. in 8th ISNI Congress; 2006 Oct 15-19; Nagoya, Japan, 167-168.
https://hdl.handle.net/21.15107/rcub_ibiss_1636

Savić D, Lavrnja I, Peković S, Šubašić SA, Jovanović S, Nikić I, Bjelobaba I, Mostarica-Stojković MB, Stošić-Grujičić S, Rakić L, Stojiljković MB. Nucleoside analogues effect on glial response in experimental autoimmune encephalomyelitis. in 8th ISNI Congress; 2006 Oct 15-19; Nagoya, Japan. 2006;:167-168.
https://hdl.handle.net/21.15107/rcub_ibiss_1636 .

Savić, Danijela, Lavrnja, Irena, Peković, Sanja, Šubašić, Sanja A, Jovanović, Sasa, Nikić, Ivana, Bjelobaba, Ivana, Mostarica-Stojković, Marija B, Stošić-Grujičić, Stanislava, Rakić, Ljubisav, Stojiljković, Mirjana B, "Nucleoside analogues effect on glial response in experimental autoimmune encephalomyelitis" in 8th ISNI Congress; 2006 Oct 15-19; Nagoya, Japan (2006):167-168,
https://hdl.handle.net/21.15107/rcub_ibiss_1636 .

TY  - JOUR
AU  - Bjelobaba, Ivana
AU  - Nedeljković, Nadežda N.
AU  - Šubašić, Sanja A
AU  - Lavrnja, Irena
AU  - Peković, Sanja
AU  - Savić, Danijela
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1627
AB  - Immunohistochemical study was performed to determine distribution of ecto-nucleotide pyrophosphatase/phosphodiesterase1 (NPP1) in adult rat forebrain. The study revealed widespread distribution of NPP1 in rat forebrain, yet with regional differences in the expression pattern and abundance. Strong NPP1 immunoreaction was detected in pyramidal cell layer of cerebral cortex and hippocampus, and in the midline regions of hypothalamus and thalamus. In many immunopositive forebrain areas, NPP1 was mainly localized at neuronal cell bodies. However, prominent immunoreaction was also detected at ependymal cells, tanycytes, endothelial cells of the capillaries and cells of the choroid plexus, suggesting that NPP1 could be involved in some highly specialized transport process.
PB  - Elsevier
T2  - Brain Research
T1  - Immunolocalization of ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) in the rat forebrain
IS  - 1
VL  - 1120
DO  - 10.1016/j.brainres.2006.08.114
SP  - 54
EP  - 63
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1627
ER  - 

@article{
author = "Bjelobaba, Ivana and Nedeljković, Nadežda N. and Šubašić, Sanja A and Lavrnja, Irena and Peković, Sanja and Savić, Danijela and Rakić, Ljubisav and Stojiljković, Mirjana B",
year = "2006",
abstract = "Immunohistochemical study was performed to determine distribution of ecto-nucleotide pyrophosphatase/phosphodiesterase1 (NPP1) in adult rat forebrain. The study revealed widespread distribution of NPP1 in rat forebrain, yet with regional differences in the expression pattern and abundance. Strong NPP1 immunoreaction was detected in pyramidal cell layer of cerebral cortex and hippocampus, and in the midline regions of hypothalamus and thalamus. In many immunopositive forebrain areas, NPP1 was mainly localized at neuronal cell bodies. However, prominent immunoreaction was also detected at ependymal cells, tanycytes, endothelial cells of the capillaries and cells of the choroid plexus, suggesting that NPP1 could be involved in some highly specialized transport process.",
publisher = "Elsevier",
journal = "Brain Research",
title = "Immunolocalization of ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) in the rat forebrain",
number = "1",
volume = "1120",
doi = "10.1016/j.brainres.2006.08.114",
pages = "54-63",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1627"
}

Bjelobaba, I., Nedeljković, N. N., Šubašić, S. A., Lavrnja, I., Peković, S., Savić, D., Rakić, L.,& Stojiljković, M. B.. (2006). Immunolocalization of ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) in the rat forebrain. in Brain Research
Elsevier., 1120(1), 54-63.
https://doi.org/10.1016/j.brainres.2006.08.114
https://hdl.handle.net/21.15107/rcub_ibiss_1627

Bjelobaba I, Nedeljković NN, Šubašić SA, Lavrnja I, Peković S, Savić D, Rakić L, Stojiljković MB. Immunolocalization of ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) in the rat forebrain. in Brain Research. 2006;1120(1):54-63.
doi:10.1016/j.brainres.2006.08.114
https://hdl.handle.net/21.15107/rcub_ibiss_1627 .

Bjelobaba, Ivana, Nedeljković, Nadežda N., Šubašić, Sanja A, Lavrnja, Irena, Peković, Sanja, Savić, Danijela, Rakić, Ljubisav, Stojiljković, Mirjana B, "Immunolocalization of ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) in the rat forebrain" in Brain Research, 1120, no. 1 (2006):54-63,
https://doi.org/10.1016/j.brainres.2006.08.114 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1627 .

TY  - JOUR
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Peković, Sanja
AU  - Šubašić, Sanja A
AU  - Mostarica-Stojković, Marija B
AU  - Stošić-Grujičić, Stanislava
AU  - Nedeljković, Nadežda N
AU  - Medić-Mijačević, Ljubica
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1714
AB  - The effect of combined treatment with ribavirin and tiazofurin on the development of experimental autoimmune encephalomyelitis, the best characterized animal model for human autoimmune disease multiple sclerosis, was investigated. The disease was induced in highly susceptible Dark Agouti rats with spinal cord homogenate in complete Freund's adjuvant. Although ribavirin or tiazofurin alone reduced the clinical and histopathological signs of experimental autoinmume encephalomyelitis, the combination of drugs achieved the same effect with significantly lower doses.
T2  - Biophysics from Molecules to Brain: In Memory of Radoslav K. (AndjusAnnals of the New York Academy of Sciences; Vol 1048; No. 1).
T1  - Combination of nucleoside analogues tiazofurin and ribavirin downregulates experimental autoimmune encephalomyelitis
DO  - 10.1196/annals.1342.047
SP  - 392
EP  - 395
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1714
ER  - 

@article{
author = "Lavrnja, Irena and Savić, Danijela and Peković, Sanja and Šubašić, Sanja A and Mostarica-Stojković, Marija B and Stošić-Grujičić, Stanislava and Nedeljković, Nadežda N and Medić-Mijačević, Ljubica and Rakić, Ljubisav and Stojiljković, Mirjana",
year = "2005",
abstract = "The effect of combined treatment with ribavirin and tiazofurin on the development of experimental autoimmune encephalomyelitis, the best characterized animal model for human autoimmune disease multiple sclerosis, was investigated. The disease was induced in highly susceptible Dark Agouti rats with spinal cord homogenate in complete Freund's adjuvant. Although ribavirin or tiazofurin alone reduced the clinical and histopathological signs of experimental autoinmume encephalomyelitis, the combination of drugs achieved the same effect with significantly lower doses.",
journal = "Biophysics from Molecules to Brain: In Memory of Radoslav K. (AndjusAnnals of the New York Academy of Sciences; Vol 1048; No. 1).",
title = "Combination of nucleoside analogues tiazofurin and ribavirin downregulates experimental autoimmune encephalomyelitis",
doi = "10.1196/annals.1342.047",
pages = "392-395",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1714"
}

Lavrnja, I., Savić, D., Peković, S., Šubašić, S. A., Mostarica-Stojković, M. B., Stošić-Grujičić, S., Nedeljković, N. N., Medić-Mijačević, L., Rakić, L.,& Stojiljković, M.. (2005). Combination of nucleoside analogues tiazofurin and ribavirin downregulates experimental autoimmune encephalomyelitis. in Biophysics from Molecules to Brain: In Memory of Radoslav K. (AndjusAnnals of the New York Academy of Sciences; Vol 1048; No. 1)., 392-395.
https://doi.org/10.1196/annals.1342.047
https://hdl.handle.net/21.15107/rcub_ibiss_1714

Lavrnja I, Savić D, Peković S, Šubašić SA, Mostarica-Stojković MB, Stošić-Grujičić S, Nedeljković NN, Medić-Mijačević L, Rakić L, Stojiljković M. Combination of nucleoside analogues tiazofurin and ribavirin downregulates experimental autoimmune encephalomyelitis. in Biophysics from Molecules to Brain: In Memory of Radoslav K. (AndjusAnnals of the New York Academy of Sciences; Vol 1048; No. 1).. 2005;:392-395.
doi:10.1196/annals.1342.047
https://hdl.handle.net/21.15107/rcub_ibiss_1714 .

Lavrnja, Irena, Savić, Danijela, Peković, Sanja, Šubašić, Sanja A, Mostarica-Stojković, Marija B, Stošić-Grujičić, Stanislava, Nedeljković, Nadežda N, Medić-Mijačević, Ljubica, Rakić, Ljubisav, Stojiljković, Mirjana, "Combination of nucleoside analogues tiazofurin and ribavirin downregulates experimental autoimmune encephalomyelitis" in Biophysics from Molecules to Brain: In Memory of Radoslav K. (AndjusAnnals of the New York Academy of Sciences; Vol 1048; No. 1). (2005):392-395,
https://doi.org/10.1196/annals.1342.047 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1714 .

TY  - JOUR
AU  - Lavrnja, Irena
AU  - Peković, Sanja
AU  - Subasić, Sanja A
AU  - Mostarica-Stojković, Marija B
AU  - Stošić-Grujičić, Stanislava
AU  - Medić-Mijačević, Ljubica
AU  - Pejanović, V
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B
PY  - 2003
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1768
AB  - The effect of ribavirin on development of experimental autoimmune encephalomyelitis (EAE) was investigated. The disease was induced in genetically susceptible Dark Agouti rats with syngeneic spinal cord homogenate in complete Freund's adjuvant (SCH-CFA). Depending on the amount of mycobacteria in CFA, the animals developed either moderate or severe EAE. Ribavirin (1-beta-Dribofuranosyl-1,2,4-triazole-3-carboxamide) was applied i.p. at a daily dosage of 30 mg/kg in two treatment protocols: from the start of immunization (preventive treatment) or from the onset of the first EAE signs after the induction (therapeutic treatment). Signs of EAE began between 7 and 9 days after induction and peaked at days 11-13. In moderate EAE (mean maximal severity score 3.33 +/- 0.21), the recovery was completed by days 23-26, whereas, in severe EAE (mean maximal severity score 4.5 +/- 0.23), obvious recovery was not detected. Preventive ribavirin treatment significantly decreased clinical signs after both moderate (score 1.75 +/- 0.25, P < 0.05) and severe (score 3.62 +/- 0.31, P < 0.015) immunization. Also, disease manifestations were reduced by therapeutic treatment of ribavirin (mean maximal severity score 2.5 +/- 0.2 vs. 3.33 +/- 0.21 in controls, P < 0.005) but less so in comparison with preventive treatment. Analysis of the effects of ribavirin on histopathologic changes in the spinal cord tissue revealed a reduction of mononuclear cell infiltrates, composed of T cells and macrophages/microglia, and the absence of demyelination, which were pronounced in control EAE animals. Beneficial effects of preventive and therapeutic treatment with ribavirin on development of EAE suggest this nucleoside analogue as a useful candidate for therapy in multiple sclerosis. (C) 2003 Wiley-Liss, Inc.
T2  - Journal of Neuroscience Research
T1  - Ribavirin reduces clinical signs and pathological changes of experimental autoimmune encephalomyelitis in dark Agouti rats
IS  - 2
VL  - 72
EP  - 278
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1768
ER  - 

@article{
author = "Lavrnja, Irena and Peković, Sanja and Subasić, Sanja A and Mostarica-Stojković, Marija B and Stošić-Grujičić, Stanislava and Medić-Mijačević, Ljubica and Pejanović, V and Rakić, Ljubisav and Stojiljković, Mirjana B",
year = "2003",
abstract = "The effect of ribavirin on development of experimental autoimmune encephalomyelitis (EAE) was investigated. The disease was induced in genetically susceptible Dark Agouti rats with syngeneic spinal cord homogenate in complete Freund's adjuvant (SCH-CFA). Depending on the amount of mycobacteria in CFA, the animals developed either moderate or severe EAE. Ribavirin (1-beta-Dribofuranosyl-1,2,4-triazole-3-carboxamide) was applied i.p. at a daily dosage of 30 mg/kg in two treatment protocols: from the start of immunization (preventive treatment) or from the onset of the first EAE signs after the induction (therapeutic treatment). Signs of EAE began between 7 and 9 days after induction and peaked at days 11-13. In moderate EAE (mean maximal severity score 3.33 +/- 0.21), the recovery was completed by days 23-26, whereas, in severe EAE (mean maximal severity score 4.5 +/- 0.23), obvious recovery was not detected. Preventive ribavirin treatment significantly decreased clinical signs after both moderate (score 1.75 +/- 0.25, P < 0.05) and severe (score 3.62 +/- 0.31, P < 0.015) immunization. Also, disease manifestations were reduced by therapeutic treatment of ribavirin (mean maximal severity score 2.5 +/- 0.2 vs. 3.33 +/- 0.21 in controls, P < 0.005) but less so in comparison with preventive treatment. Analysis of the effects of ribavirin on histopathologic changes in the spinal cord tissue revealed a reduction of mononuclear cell infiltrates, composed of T cells and macrophages/microglia, and the absence of demyelination, which were pronounced in control EAE animals. Beneficial effects of preventive and therapeutic treatment with ribavirin on development of EAE suggest this nucleoside analogue as a useful candidate for therapy in multiple sclerosis. (C) 2003 Wiley-Liss, Inc.",
journal = "Journal of Neuroscience Research",
title = "Ribavirin reduces clinical signs and pathological changes of experimental autoimmune encephalomyelitis in dark Agouti rats",
number = "2",
volume = "72",
pages = "278",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1768"
}

Lavrnja, I., Peković, S., Subasić, S. A., Mostarica-Stojković, M. B., Stošić-Grujičić, S., Medić-Mijačević, L., Pejanović, V., Rakić, L.,& Stojiljković, M. B.. (2003). Ribavirin reduces clinical signs and pathological changes of experimental autoimmune encephalomyelitis in dark Agouti rats. in Journal of Neuroscience Research, 72(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1768

Lavrnja I, Peković S, Subasić SA, Mostarica-Stojković MB, Stošić-Grujičić S, Medić-Mijačević L, Pejanović V, Rakić L, Stojiljković MB. Ribavirin reduces clinical signs and pathological changes of experimental autoimmune encephalomyelitis in dark Agouti rats. in Journal of Neuroscience Research. 2003;72(2):null-278.
https://hdl.handle.net/21.15107/rcub_ibiss_1768 .

Lavrnja, Irena, Peković, Sanja, Subasić, Sanja A, Mostarica-Stojković, Marija B, Stošić-Grujičić, Stanislava, Medić-Mijačević, Ljubica, Pejanović, V, Rakić, Ljubisav, Stojiljković, Mirjana B, "Ribavirin reduces clinical signs and pathological changes of experimental autoimmune encephalomyelitis in dark Agouti rats" in Journal of Neuroscience Research, 72, no. 2 (2003),
https://hdl.handle.net/21.15107/rcub_ibiss_1768 .