TY  - JOUR
AU  - Ristic, Aleksandar J.
AU  - Savić, Danijela
AU  - Sokic, Dragoslav
AU  - Pristov, Jelena Bogdanovic
AU  - Brkljačić, Jelena
AU  - Bascarevic, Vladimir
AU  - Raicevic, Savo
AU  - Savic, Slobodan
AU  - Spasojevic, Ivan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1960
AB  - ObjectiveTo examine antioxidative system in hippocampi of patients with
   mesial temporal lobe epilepsy associated with hippocampal sclerosis
   (mTLE-HS).
   MethodsActivity and levels of antioxidative enzymescatalase (CAT),
   glutathione peroxidase (GPx), glutathione reductase (GR), manganese
   superoxide dismutase (MnSOD), and copper-zinc superoxide dismutase
   (CuZnSOD)were assessed in hippocampi of nine pharmacoresistant mTLE-HS
   patients (mean age 37.7{[}standard deviation] 6.6years) who underwent
   amygdalohippocampectomy, and in 10 hippocampi obtained via autopsy from
   five neurologically intact controls (mean age 34.49.0years). Subfield
   and cellular (neuron/astrocyte) distribution of CAT, GPx, and MnSOD was
   analyzed in detail using immunohistochemical staining.
   ResultsSclerotic hippocampi showed drastically increased activity of
   hydrogen peroxide-removing enzymes, CAT (p<0.001), GPx (p<0.001), and GR
   (p<0.001), and significantly higher protein levels of CAT (p=0.006), GPx
   (p=0.040), GR (p=0.024), and MnSOD (p=0.004), compared to controls. CAT
   immunofluorescence was located mainly in neurons in both controls and
   HS. Control hippocampi showed GPx staining in blood vessels and CA
   neurons. In HS, GPx-rich loci, representing bundles of astrocytes,
   emerged in different hippocampal regions, whereas the number of
   GPx-positive vessels was drastically decreased. Neurons with abnormal
   morphology and strong MnSOD immunofluorescence were present in all
   neuronal layers in HS. Small autofluorescent deposits, most likely
   lipofuscin, were observed, along with astrogliosis, in CA1 in HS.
   SignificanceAntioxidative system is upregulated in HS. This documents,
   for the first time, that epileptogenic hippocampi are exposed to
   oxidative stress. Our findings provide a basis for understanding the
   potential involvement of redox alterations in the pathology of epilepsy,
   and may open new pharmacologic perspectives for mTLE-HS treatment.
T2  - Epilepsia
T1  - Hippocampal antioxidative system in mesial temporal lobe epilepsy
IS  - 5
VL  - 56
DO  - 10.1111/epi.12981
SP  - 789
EP  - 799
ER  - 

@article{
author = "Ristic, Aleksandar J. and Savić, Danijela and Sokic, Dragoslav and Pristov, Jelena Bogdanovic and Brkljačić, Jelena and Bascarevic, Vladimir and Raicevic, Savo and Savic, Slobodan and Spasojevic, Ivan",
year = "2015",
abstract = "ObjectiveTo examine antioxidative system in hippocampi of patients with
   mesial temporal lobe epilepsy associated with hippocampal sclerosis
   (mTLE-HS).
   MethodsActivity and levels of antioxidative enzymescatalase (CAT),
   glutathione peroxidase (GPx), glutathione reductase (GR), manganese
   superoxide dismutase (MnSOD), and copper-zinc superoxide dismutase
   (CuZnSOD)were assessed in hippocampi of nine pharmacoresistant mTLE-HS
   patients (mean age 37.7{[}standard deviation] 6.6years) who underwent
   amygdalohippocampectomy, and in 10 hippocampi obtained via autopsy from
   five neurologically intact controls (mean age 34.49.0years). Subfield
   and cellular (neuron/astrocyte) distribution of CAT, GPx, and MnSOD was
   analyzed in detail using immunohistochemical staining.
   ResultsSclerotic hippocampi showed drastically increased activity of
   hydrogen peroxide-removing enzymes, CAT (p<0.001), GPx (p<0.001), and GR
   (p<0.001), and significantly higher protein levels of CAT (p=0.006), GPx
   (p=0.040), GR (p=0.024), and MnSOD (p=0.004), compared to controls. CAT
   immunofluorescence was located mainly in neurons in both controls and
   HS. Control hippocampi showed GPx staining in blood vessels and CA
   neurons. In HS, GPx-rich loci, representing bundles of astrocytes,
   emerged in different hippocampal regions, whereas the number of
   GPx-positive vessels was drastically decreased. Neurons with abnormal
   morphology and strong MnSOD immunofluorescence were present in all
   neuronal layers in HS. Small autofluorescent deposits, most likely
   lipofuscin, were observed, along with astrogliosis, in CA1 in HS.
   SignificanceAntioxidative system is upregulated in HS. This documents,
   for the first time, that epileptogenic hippocampi are exposed to
   oxidative stress. Our findings provide a basis for understanding the
   potential involvement of redox alterations in the pathology of epilepsy,
   and may open new pharmacologic perspectives for mTLE-HS treatment.",
journal = "Epilepsia",
title = "Hippocampal antioxidative system in mesial temporal lobe epilepsy",
number = "5",
volume = "56",
doi = "10.1111/epi.12981",
pages = "789-799"
}

Ristic, A. J., Savić, D., Sokic, D., Pristov, J. B., Brkljačić, J., Bascarevic, V., Raicevic, S., Savic, S.,& Spasojevic, I.. (2015). Hippocampal antioxidative system in mesial temporal lobe epilepsy. in Epilepsia, 56(5), 789-799.
https://doi.org/10.1111/epi.12981

Ristic AJ, Savić D, Sokic D, Pristov JB, Brkljačić J, Bascarevic V, Raicevic S, Savic S, Spasojevic I. Hippocampal antioxidative system in mesial temporal lobe epilepsy. in Epilepsia. 2015;56(5):789-799.
doi:10.1111/epi.12981 .

Ristic, Aleksandar J., Savić, Danijela, Sokic, Dragoslav, Pristov, Jelena Bogdanovic, Brkljačić, Jelena, Bascarevic, Vladimir, Raicevic, Savo, Savic, Slobodan, Spasojevic, Ivan, "Hippocampal antioxidative system in mesial temporal lobe epilepsy" in Epilepsia, 56, no. 5 (2015):789-799,
https://doi.org/10.1111/epi.12981 . .

TY  - JOUR
AU  - Ristic, Aleksandar J.
AU  - Sokic, Dragoslav
AU  - Bascarevic, Vladimir
AU  - Spasic, Snezana
AU  - Vojvodic, Nikola
AU  - Savic, Slobodan
AU  - Raicevic, Savo
AU  - Kovacevic, Masa
AU  - Savić, Danijela
AU  - Spasojevic, Ivan
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2214
AB  - An altered metal and electrolyte profile has been implicated in the
   pathologic mechanisms of chronic epilepsy; however, no study has
   comprehensively measured hippocampal concentrations of these elements in
   patients with mesial temporal lobe epilepsy and hippocampal sclerosis
   (mTLE-HS). We therefore analyzed hippocampi of 24 patients with
   drug-resistant mTLE-HS (mean age 35.69.4years) who underwent anterior
   temporal lobe resection and amygdalohippocampectomy and 17 hippocampi
   obtained by autopsy from 13 controls (mean age 40.5 +/- 12.9years),
   using inductively coupled plasma optical emission spectrometry
   (ICP-OES). Epileptic hippocampi showed significantly lower
   concentrations (g/g of tissue) of copper (HS: 2.34 +/- 0.12; control
   {[}C]: 3.57 +/- 0.33; p<0.001), manganese (HS: 0.205 +/- 0.030; C: 0.409
   +/- 0.064; p=0.004), and potassium (HS: 2,001 +/- 59; C: 2,322 +/- 61;
   p<0.001), and increased sodium levels (HS: 1,131 +/- 22; C: 1,040 +/-
   25; p=0.010). Zinc, iron, calcium, and magnesium levels did not differ
   in HS and controls. In summary, copper and manganese levels are
   deficient, whereas iron level is unchanged in hippocampi from patients
   with mTLE-HS. Our results provide a basis for understanding the
   potential involvement of different metals and electrolytes in the
   pathology of HS. A PowerPoint slide summarizing this article is
   available for download in the Supporting Information section .
T2  - Epilepsia
T1  - Metals and electrolytes in sclerotic hippocampi in patients with
 drug-resistant mesial temporal lobe epilepsy
IS  - 5
VL  - 55
DO  - 10.1111/epi.12593
SP  - E34
EP  - E37
ER  - 

@article{
author = "Ristic, Aleksandar J. and Sokic, Dragoslav and Bascarevic, Vladimir and Spasic, Snezana and Vojvodic, Nikola and Savic, Slobodan and Raicevic, Savo and Kovacevic, Masa and Savić, Danijela and Spasojevic, Ivan",
year = "2014",
abstract = "An altered metal and electrolyte profile has been implicated in the
   pathologic mechanisms of chronic epilepsy; however, no study has
   comprehensively measured hippocampal concentrations of these elements in
   patients with mesial temporal lobe epilepsy and hippocampal sclerosis
   (mTLE-HS). We therefore analyzed hippocampi of 24 patients with
   drug-resistant mTLE-HS (mean age 35.69.4years) who underwent anterior
   temporal lobe resection and amygdalohippocampectomy and 17 hippocampi
   obtained by autopsy from 13 controls (mean age 40.5 +/- 12.9years),
   using inductively coupled plasma optical emission spectrometry
   (ICP-OES). Epileptic hippocampi showed significantly lower
   concentrations (g/g of tissue) of copper (HS: 2.34 +/- 0.12; control
   {[}C]: 3.57 +/- 0.33; p<0.001), manganese (HS: 0.205 +/- 0.030; C: 0.409
   +/- 0.064; p=0.004), and potassium (HS: 2,001 +/- 59; C: 2,322 +/- 61;
   p<0.001), and increased sodium levels (HS: 1,131 +/- 22; C: 1,040 +/-
   25; p=0.010). Zinc, iron, calcium, and magnesium levels did not differ
   in HS and controls. In summary, copper and manganese levels are
   deficient, whereas iron level is unchanged in hippocampi from patients
   with mTLE-HS. Our results provide a basis for understanding the
   potential involvement of different metals and electrolytes in the
   pathology of HS. A PowerPoint slide summarizing this article is
   available for download in the Supporting Information section .",
journal = "Epilepsia",
title = "Metals and electrolytes in sclerotic hippocampi in patients with
 drug-resistant mesial temporal lobe epilepsy",
number = "5",
volume = "55",
doi = "10.1111/epi.12593",
pages = "E34-E37"
}

Ristic, A. J., Sokic, D., Bascarevic, V., Spasic, S., Vojvodic, N., Savic, S., Raicevic, S., Kovacevic, M., Savić, D.,& Spasojevic, I.. (2014). Metals and electrolytes in sclerotic hippocampi in patients with
 drug-resistant mesial temporal lobe epilepsy. in Epilepsia, 55(5), E34-E37.
https://doi.org/10.1111/epi.12593

Ristic AJ, Sokic D, Bascarevic V, Spasic S, Vojvodic N, Savic S, Raicevic S, Kovacevic M, Savić D, Spasojevic I. Metals and electrolytes in sclerotic hippocampi in patients with
 drug-resistant mesial temporal lobe epilepsy. in Epilepsia. 2014;55(5):E34-E37.
doi:10.1111/epi.12593 .

Ristic, Aleksandar J., Sokic, Dragoslav, Bascarevic, Vladimir, Spasic, Snezana, Vojvodic, Nikola, Savic, Slobodan, Raicevic, Savo, Kovacevic, Masa, Savić, Danijela, Spasojevic, Ivan, "Metals and electrolytes in sclerotic hippocampi in patients with
 drug-resistant mesial temporal lobe epilepsy" in Epilepsia, 55, no. 5 (2014):E34-E37,
https://doi.org/10.1111/epi.12593 . .