TY  - CONF
AU  - Potrebić, Milica
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Milenković, Dragan
AU  - Pendoski, Laze
AU  - Jakovljević, Vladimir
AU  - Milošević, Verica
PY  - 2018
UR  - http://www.enea2018.com/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6041
AB  - Objective: The complex process of aging can be defined as progressive loss of the ability to maintain
tissue homeostasis, which may cause many pathological issues, like depression, Alzheimer’s diseases
or cognitive deficits. Due to their various established health effects, citrus flavanones, naringenin
(NAR) and hesperetin (HES), could have important protective role against development and progression of many age-related diseases.The aim of this study was to examine the effects of these flavanones on pituitary-adrenocortical axis in the rat model of old age (24 months old Wistar rats), Methods: The experimental groups included control group, and naringenin and hesperetin treated groups. NAR and HES were administrated orally (15 mg/kg b. m.) during 4 weeks, while control group received sunflower oil as a vehicle. Concentrations of adrenocorticotropic (ACTH) hormone in pituitary ACTH cells (reflected by relative intensity of fluorescence) and blood levels of ACTH and corticosterone in experimental rats were determined semi-quantitatively and by using the immunoassays, respectively. Results: Results have shown that both blood concentrations of ACTH and corticosterone were markedly lower in NAR [51% and 39%, respectively (P<0.05)] and HES treated group [69% and 65%, respectively (P<0.05)] in comparison with the control group. The relative intensity of ACTH immunofluorescence signal was the highest in the control group, while in NAR and HES groups was significantly lower [39% and 65%, respectively (P<0.05)]. Conclusion: This study has shown that citrus flavanones affect pituitary-adrenocortical axis by decreasing ACTH levels in adrenocorticotropic cells and blood concentrations of ACTH and corticosterone, as well. Since the increased activation of this axis in elderly is associated with development of many aging-related neurophysiological issues, bioactive flavanones may be used in their prevention or delay.
PB  - Altdorf: EndoScience Endokrinologie Service GmbH
C3  - 18th Congress of the European Neuroendocrine Association: Abstracts; 2018 Oct 17-20; Wrocław, Poland
T1  - Citrus Flavanones, Naringenin and Hesperetin, decrease ACTH and Corticosterone Levels in Old Rats
SP  - 27
EP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6041
ER  - 

@conference{
author = "Potrebić, Milica and Ajdžanović, Vladimir and Miler, Marko and Milenković, Dragan and Pendoski, Laze and Jakovljević, Vladimir and Milošević, Verica",
year = "2018",
abstract = "Objective: The complex process of aging can be defined as progressive loss of the ability to maintain
tissue homeostasis, which may cause many pathological issues, like depression, Alzheimer’s diseases
or cognitive deficits. Due to their various established health effects, citrus flavanones, naringenin
(NAR) and hesperetin (HES), could have important protective role against development and progression of many age-related diseases.The aim of this study was to examine the effects of these flavanones on pituitary-adrenocortical axis in the rat model of old age (24 months old Wistar rats), Methods: The experimental groups included control group, and naringenin and hesperetin treated groups. NAR and HES were administrated orally (15 mg/kg b. m.) during 4 weeks, while control group received sunflower oil as a vehicle. Concentrations of adrenocorticotropic (ACTH) hormone in pituitary ACTH cells (reflected by relative intensity of fluorescence) and blood levels of ACTH and corticosterone in experimental rats were determined semi-quantitatively and by using the immunoassays, respectively. Results: Results have shown that both blood concentrations of ACTH and corticosterone were markedly lower in NAR [51% and 39%, respectively (P<0.05)] and HES treated group [69% and 65%, respectively (P<0.05)] in comparison with the control group. The relative intensity of ACTH immunofluorescence signal was the highest in the control group, while in NAR and HES groups was significantly lower [39% and 65%, respectively (P<0.05)]. Conclusion: This study has shown that citrus flavanones affect pituitary-adrenocortical axis by decreasing ACTH levels in adrenocorticotropic cells and blood concentrations of ACTH and corticosterone, as well. Since the increased activation of this axis in elderly is associated with development of many aging-related neurophysiological issues, bioactive flavanones may be used in their prevention or delay.",
publisher = "Altdorf: EndoScience Endokrinologie Service GmbH",
journal = "18th Congress of the European Neuroendocrine Association: Abstracts; 2018 Oct 17-20; Wrocław, Poland",
title = "Citrus Flavanones, Naringenin and Hesperetin, decrease ACTH and Corticosterone Levels in Old Rats",
pages = "27-28",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6041"
}

Potrebić, M., Ajdžanović, V., Miler, M., Milenković, D., Pendoski, L., Jakovljević, V.,& Milošević, V.. (2018). Citrus Flavanones, Naringenin and Hesperetin, decrease ACTH and Corticosterone Levels in Old Rats. in 18th Congress of the European Neuroendocrine Association: Abstracts; 2018 Oct 17-20; Wrocław, Poland
Altdorf: EndoScience Endokrinologie Service GmbH., 27-28.
https://hdl.handle.net/21.15107/rcub_ibiss_6041

Potrebić M, Ajdžanović V, Miler M, Milenković D, Pendoski L, Jakovljević V, Milošević V. Citrus Flavanones, Naringenin and Hesperetin, decrease ACTH and Corticosterone Levels in Old Rats. in 18th Congress of the European Neuroendocrine Association: Abstracts; 2018 Oct 17-20; Wrocław, Poland. 2018;:27-28.
https://hdl.handle.net/21.15107/rcub_ibiss_6041 .

Potrebić, Milica, Ajdžanović, Vladimir, Miler, Marko, Milenković, Dragan, Pendoski, Laze, Jakovljević, Vladimir, Milošević, Verica, "Citrus Flavanones, Naringenin and Hesperetin, decrease ACTH and Corticosterone Levels in Old Rats" in 18th Congress of the European Neuroendocrine Association: Abstracts; 2018 Oct 17-20; Wrocław, Poland (2018):27-28,
https://hdl.handle.net/21.15107/rcub_ibiss_6041 .

TY  - CONF
AU  - Jarić, Ivana
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Pendoski, Laze
AU  - Žikić, Dragan
AU  - Ušćebrka, Gordana
AU  - Milošević, Verica
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6037
AB  - Diosgenin is bioactive, steroidal saponin from Dioscorea viliosa (Wild Yam) and appears to be novel promising substance for the treatment of menopausal symptoms. In addition, diosgenin may manipulate the endogenous steroid hormone levels and may affect the numerous oxidative stress pathways/molecules. Since decline in the steroid hormone levels as well as increase in oxidative stress in the ovaries play an integral part of the menopause, this study aimed to investigate the effects of diosgenin on production of estradiol and progesterone, as well as to investigated the effects of diosgenin on the expression of genes encoding the antioxidant enzymes (Cu, Zn-superoxide dismutase (CuZnSOD), Mn-superoxide dismutase (MnSOD), glutathione peroxidase (GPX), and catalase (CAD) in ovary of acyclic-middle aged female rats. Middle-aged (l3-months-old) acyclic female Wistar rats in constant diestrus stage, which represent tractable model to human menopause, were divided into control and diosgenin treated groups.Diosgenin (100 mgfl<g b.w.lday) was orally applied for 4 weeks, while C group received the vehicle alone. In our experimental model, diosgenin administration to acyclic middle-aged rats significantly increased estradiol and progesterone serum concentrations when compared to the corresponding control. Gene expression analysis revealed that diosgenin significantly down-regulate all examined enzymes involved in antioxidative defense. The down-regulation were especially pronounced in CuZnSOD and CAT, indicating that the diosgenin may decrease the pool of reactive oxygen species in the ovary directly due and may act as free radical scavengers. Therefore, our results indicate beneficial potential of diosgenin in the middle-aged rat ovary.
PB  - Skopje: Faculty of Veterinary Medicine
C3  - Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia
T1  - Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats
SP  - 147
EP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6037
ER  - 

@conference{
author = "Jarić, Ivana and Miler, Marko and Ajdžanović, Vladimir and Živanović, Jasmina and Pendoski, Laze and Žikić, Dragan and Ušćebrka, Gordana and Milošević, Verica",
year = "2018",
abstract = "Diosgenin is bioactive, steroidal saponin from Dioscorea viliosa (Wild Yam) and appears to be novel promising substance for the treatment of menopausal symptoms. In addition, diosgenin may manipulate the endogenous steroid hormone levels and may affect the numerous oxidative stress pathways/molecules. Since decline in the steroid hormone levels as well as increase in oxidative stress in the ovaries play an integral part of the menopause, this study aimed to investigate the effects of diosgenin on production of estradiol and progesterone, as well as to investigated the effects of diosgenin on the expression of genes encoding the antioxidant enzymes (Cu, Zn-superoxide dismutase (CuZnSOD), Mn-superoxide dismutase (MnSOD), glutathione peroxidase (GPX), and catalase (CAD) in ovary of acyclic-middle aged female rats. Middle-aged (l3-months-old) acyclic female Wistar rats in constant diestrus stage, which represent tractable model to human menopause, were divided into control and diosgenin treated groups.Diosgenin (100 mgfl<g b.w.lday) was orally applied for 4 weeks, while C group received the vehicle alone. In our experimental model, diosgenin administration to acyclic middle-aged rats significantly increased estradiol and progesterone serum concentrations when compared to the corresponding control. Gene expression analysis revealed that diosgenin significantly down-regulate all examined enzymes involved in antioxidative defense. The down-regulation were especially pronounced in CuZnSOD and CAT, indicating that the diosgenin may decrease the pool of reactive oxygen species in the ovary directly due and may act as free radical scavengers. Therefore, our results indicate beneficial potential of diosgenin in the middle-aged rat ovary.",
publisher = "Skopje: Faculty of Veterinary Medicine",
journal = "Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia",
title = "Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats",
pages = "147-147",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6037"
}

Jarić, I., Miler, M., Ajdžanović, V., Živanović, J., Pendoski, L., Žikić, D., Ušćebrka, G.,& Milošević, V.. (2018). Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats. in Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia
Skopje: Faculty of Veterinary Medicine., 147-147.
https://hdl.handle.net/21.15107/rcub_ibiss_6037

Jarić I, Miler M, Ajdžanović V, Živanović J, Pendoski L, Žikić D, Ušćebrka G, Milošević V. Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats. in Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia. 2018;:147-147.
https://hdl.handle.net/21.15107/rcub_ibiss_6037 .

Jarić, Ivana, Miler, Marko, Ajdžanović, Vladimir, Živanović, Jasmina, Pendoski, Laze, Žikić, Dragan, Ušćebrka, Gordana, Milošević, Verica, "Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats" in Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia (2018):147-147,
https://hdl.handle.net/21.15107/rcub_ibiss_6037 .

TY  - CONF
AU  - Ajdžanović, Vladimir
AU  - Jakovljević, Vladimir
AU  - Miler, Marko
AU  - Ristić, Nataša
AU  - Potrebić, Milica
AU  - Sretenović, Jasmina
AU  - Popovski Perčinić, Florina
AU  - Pendoski, Laze
AU  - Milošević, Verica
PY  - 2018
UR  - http://physiology.org.rs/4thCPS.php
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6035
AB  - The aim of this study was to investigate the immunohistomorphometric parameters of follicle-stimulating hormone (TSH) and luteinizing hormone (I.H) cells after testosterone application in an animal model of the andropause. Middle-aged Wistar rats were divided into orchidectomized (Or) and testosterone treated orchidectomized (OrxT) groups. Testosterone propionate (5 mg/kg b.m. (day) was administered for three weeks. while Or group received the vehicle alone.
Immunohistochemically stained FSH and I cells were subjected to the morphometric and optical density-related analysis. while circulating concentration of testosterone was measured by the immunoassay. Serum concentration of testosterone was increased (ps0.05) by 24 fold in comparison with Orx group.
Testosterone treatment did not cause significant changes of body mass or absolute and relative pituitary weights compared to Or group. The volume of FSH and LH cells was decreased (ps0.05) by 51.3% and 56.6% respectively, in comparison with Or rats. Relative volume density of FSI and LE cells was also decreased (p<0.05) by 54.0% and 72.8% respectively, compared to Or group. Results related to the optical density of gonadotropic cells were in line with the morphometric findings i.e. this parameter of FSH and LH cells was decreased (P«0.05) by 25.7% and 16.2% respectively, in comparison with Or rats. In conclusion. applied testosterone decreased morphometric parameters and optical density of gonadotropic cells in Or rats.
PB  - Belgrade : Serbian Physiological Society, 2018 (Lukovo : Galaksijanis)
C3  - Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia
T1  - Testosterone effects on the pituitary gonadotropic cells in an animal model of the andropause
SP  - 118
EP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6035
ER  - 

@conference{
author = "Ajdžanović, Vladimir and Jakovljević, Vladimir and Miler, Marko and Ristić, Nataša and Potrebić, Milica and Sretenović, Jasmina and Popovski Perčinić, Florina and Pendoski, Laze and Milošević, Verica",
year = "2018",
abstract = "The aim of this study was to investigate the immunohistomorphometric parameters of follicle-stimulating hormone (TSH) and luteinizing hormone (I.H) cells after testosterone application in an animal model of the andropause. Middle-aged Wistar rats were divided into orchidectomized (Or) and testosterone treated orchidectomized (OrxT) groups. Testosterone propionate (5 mg/kg b.m. (day) was administered for three weeks. while Or group received the vehicle alone.
Immunohistochemically stained FSH and I cells were subjected to the morphometric and optical density-related analysis. while circulating concentration of testosterone was measured by the immunoassay. Serum concentration of testosterone was increased (ps0.05) by 24 fold in comparison with Orx group.
Testosterone treatment did not cause significant changes of body mass or absolute and relative pituitary weights compared to Or group. The volume of FSH and LH cells was decreased (ps0.05) by 51.3% and 56.6% respectively, in comparison with Or rats. Relative volume density of FSI and LE cells was also decreased (p<0.05) by 54.0% and 72.8% respectively, compared to Or group. Results related to the optical density of gonadotropic cells were in line with the morphometric findings i.e. this parameter of FSH and LH cells was decreased (P«0.05) by 25.7% and 16.2% respectively, in comparison with Or rats. In conclusion. applied testosterone decreased morphometric parameters and optical density of gonadotropic cells in Or rats.",
publisher = "Belgrade : Serbian Physiological Society, 2018 (Lukovo : Galaksijanis)",
journal = "Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia",
title = "Testosterone effects on the pituitary gonadotropic cells in an animal model of the andropause",
pages = "118-118",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6035"
}

Ajdžanović, V., Jakovljević, V., Miler, M., Ristić, N., Potrebić, M., Sretenović, J., Popovski Perčinić, F., Pendoski, L.,& Milošević, V.. (2018). Testosterone effects on the pituitary gonadotropic cells in an animal model of the andropause. in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia
Belgrade : Serbian Physiological Society, 2018 (Lukovo : Galaksijanis)., 118-118.
https://hdl.handle.net/21.15107/rcub_ibiss_6035

Ajdžanović V, Jakovljević V, Miler M, Ristić N, Potrebić M, Sretenović J, Popovski Perčinić F, Pendoski L, Milošević V. Testosterone effects on the pituitary gonadotropic cells in an animal model of the andropause. in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia. 2018;:118-118.
https://hdl.handle.net/21.15107/rcub_ibiss_6035 .

Ajdžanović, Vladimir, Jakovljević, Vladimir, Miler, Marko, Ristić, Nataša, Potrebić, Milica, Sretenović, Jasmina, Popovski Perčinić, Florina, Pendoski, Laze, Milošević, Verica, "Testosterone effects on the pituitary gonadotropic cells in an animal model of the andropause" in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia (2018):118-118,
https://hdl.handle.net/21.15107/rcub_ibiss_6035 .