TY  - CONF
AU  - Pavković, Željko
AU  - Potrebić, Milica
AU  - Dubljević, Olga
AU  - Srbovan, Maja
AU  - Pešić, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5832
AB  - Adolescence is a period when alcohol abuse is more likely to occur. Use of isoflurane
(a volatile anesthetic) in adolescence has been experimentally shown to alter
responses to ethanol, implying that general exposure to anesthetics during adolescence
may be an environmental risk factor contributing to increased susceptibility to alcohol
use disorder (AUD). Our previous work showed that propofol (intravenous anesthetic)
anesthesia exposure (PAE) in adolescence favors risk-taking and preference for
novel/intense stimuli. However, its influence on alcohol consumption hasn’t been
examined. Therefore, we aimed to investigate whether PAE in adolescence alters
alcohol consumption in alcohol-naïve rats.
Male Wistar rats aged 35 and 50 postnatal days (early-to-mid and mid-to-late
adolescence, respectively) were injected with anesthetic dose of propofol emulsion
(75mg/kg) or saline, separated into individual cages and exposed to two-bottle choice
paradigm (allowing free access to food and two bottles, one containing 2,5% ethanol
in 1% sucrose solution and the other containing tap water). Liquid consumption was
measured for 4 constitutive days. Obtained data showed no preference for alcohol in
all analyzed experimental groups. Moreover, the tendency for alcohol intake was
significantly reduced during first 24h after PAE in early-to-mid adolescent rats.
Overall, our study shows that PAE doesn’t increase voluntary alcohol intake in
peripubertal rats used as a rodent model of adolescence. The obtained findings do not
support the view that exposure to general anesthetics in adolescence is a risk factor for
AUD and suggest that a detailed analysis of the range of anesthetics is needed before
generalizing the data.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Propofol reduces the tendency for alcohol consumption in adolescent rats
SP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5832
ER  - 

@conference{
author = "Pavković, Željko and Potrebić, Milica and Dubljević, Olga and Srbovan, Maja and Pešić, Vesna",
year = "2023",
abstract = "Adolescence is a period when alcohol abuse is more likely to occur. Use of isoflurane
(a volatile anesthetic) in adolescence has been experimentally shown to alter
responses to ethanol, implying that general exposure to anesthetics during adolescence
may be an environmental risk factor contributing to increased susceptibility to alcohol
use disorder (AUD). Our previous work showed that propofol (intravenous anesthetic)
anesthesia exposure (PAE) in adolescence favors risk-taking and preference for
novel/intense stimuli. However, its influence on alcohol consumption hasn’t been
examined. Therefore, we aimed to investigate whether PAE in adolescence alters
alcohol consumption in alcohol-naïve rats.
Male Wistar rats aged 35 and 50 postnatal days (early-to-mid and mid-to-late
adolescence, respectively) were injected with anesthetic dose of propofol emulsion
(75mg/kg) or saline, separated into individual cages and exposed to two-bottle choice
paradigm (allowing free access to food and two bottles, one containing 2,5% ethanol
in 1% sucrose solution and the other containing tap water). Liquid consumption was
measured for 4 constitutive days. Obtained data showed no preference for alcohol in
all analyzed experimental groups. Moreover, the tendency for alcohol intake was
significantly reduced during first 24h after PAE in early-to-mid adolescent rats.
Overall, our study shows that PAE doesn’t increase voluntary alcohol intake in
peripubertal rats used as a rodent model of adolescence. The obtained findings do not
support the view that exposure to general anesthetics in adolescence is a risk factor for
AUD and suggest that a detailed analysis of the range of anesthetics is needed before
generalizing the data.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Propofol reduces the tendency for alcohol consumption in adolescent rats",
pages = "70",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5832"
}

Pavković, Ž., Potrebić, M., Dubljević, O., Srbovan, M.,& Pešić, V.. (2023). Propofol reduces the tendency for alcohol consumption in adolescent rats. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 70.
https://hdl.handle.net/21.15107/rcub_ibiss_5832

Pavković Ž, Potrebić M, Dubljević O, Srbovan M, Pešić V. Propofol reduces the tendency for alcohol consumption in adolescent rats. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:70.
https://hdl.handle.net/21.15107/rcub_ibiss_5832 .

Pavković, Željko, Potrebić, Milica, Dubljević, Olga, Srbovan, Maja, Pešić, Vesna, "Propofol reduces the tendency for alcohol consumption in adolescent rats" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):70,
https://hdl.handle.net/21.15107/rcub_ibiss_5832 .

TY  - CONF
AU  - Potrebić, Milica
AU  - Pavković, Željko
AU  - Srbovan, Maja
AU  - Dubljević, Olga
AU  - Pešić, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5831
AB  - Aim: Microglia are macrophages of the brain with a fundamental role in neuronal
homeostasis. Stress-induced activation of microglia leads to rapid morphological
changes and it is implicated that these alternations could mediate different aspects of
early-life stress psychopathologies. We aimed to examine morphological response of
hippocampal microglia to social isolation using peripubertal rats as a rodent model of
adolescence.
Methods: Male peripubertal Wistar rats were randomly selected for group-housing
(GH; n=3 per cage) or single-housing (SH; n=1 per cage) at postnatal day 29. After
1st and 2nd week of defined housing animals῾ brains were sampled, and used for
microglia iba-1 fluorescent labelling and morphology analysis: one filed per section
per animal (n=4 animals per group) in hippocampal dentate gyrus (HDG). Data for
microglia cell shape (FracLac plugin, ImageJ) and cell ramification (Analyze Skeleton
plugin, ImageJ) were collected from single cells in photomicrograph visualized with a
confocal microscope (Leica Microsystems).
Results: Compared to GH peers, SH rats had lower microglial cell density in the HDG
after the 1st week of defined housing, which was not evident after the 2nd week. Other
parameters of microglia shape and ramification showed no difference between the
groups in neither of the two time points examined.
Conclusions: Obtained results showed that change in the cell density in the HDG was
the only and transient change in microglia morphology due to the social isolation
during adolescence. Further examination of other stress-related brain regions is
needed to define in depth the relationship between the microglia phenotype and earlylife
stress conditions.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Microglial morphological response to the lack of direct social contact in periadolescent rats
SP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5831
ER  - 

@conference{
author = "Potrebić, Milica and Pavković, Željko and Srbovan, Maja and Dubljević, Olga and Pešić, Vesna",
year = "2023",
abstract = "Aim: Microglia are macrophages of the brain with a fundamental role in neuronal
homeostasis. Stress-induced activation of microglia leads to rapid morphological
changes and it is implicated that these alternations could mediate different aspects of
early-life stress psychopathologies. We aimed to examine morphological response of
hippocampal microglia to social isolation using peripubertal rats as a rodent model of
adolescence.
Methods: Male peripubertal Wistar rats were randomly selected for group-housing
(GH; n=3 per cage) or single-housing (SH; n=1 per cage) at postnatal day 29. After
1st and 2nd week of defined housing animals῾ brains were sampled, and used for
microglia iba-1 fluorescent labelling and morphology analysis: one filed per section
per animal (n=4 animals per group) in hippocampal dentate gyrus (HDG). Data for
microglia cell shape (FracLac plugin, ImageJ) and cell ramification (Analyze Skeleton
plugin, ImageJ) were collected from single cells in photomicrograph visualized with a
confocal microscope (Leica Microsystems).
Results: Compared to GH peers, SH rats had lower microglial cell density in the HDG
after the 1st week of defined housing, which was not evident after the 2nd week. Other
parameters of microglia shape and ramification showed no difference between the
groups in neither of the two time points examined.
Conclusions: Obtained results showed that change in the cell density in the HDG was
the only and transient change in microglia morphology due to the social isolation
during adolescence. Further examination of other stress-related brain regions is
needed to define in depth the relationship between the microglia phenotype and earlylife
stress conditions.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Microglial morphological response to the lack of direct social contact in periadolescent rats",
pages = "108",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5831"
}

Potrebić, M., Pavković, Ž., Srbovan, M., Dubljević, O.,& Pešić, V.. (2023). Microglial morphological response to the lack of direct social contact in periadolescent rats. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 108.
https://hdl.handle.net/21.15107/rcub_ibiss_5831

Potrebić M, Pavković Ž, Srbovan M, Dubljević O, Pešić V. Microglial morphological response to the lack of direct social contact in periadolescent rats. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:108.
https://hdl.handle.net/21.15107/rcub_ibiss_5831 .

Potrebić, Milica, Pavković, Željko, Srbovan, Maja, Dubljević, Olga, Pešić, Vesna, "Microglial morphological response to the lack of direct social contact in periadolescent rats" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):108,
https://hdl.handle.net/21.15107/rcub_ibiss_5831 .

TY  - CONF
AU  - Dubljević, Olga
AU  - Pavković, Željko
AU  - Srbovan, Maja
AU  - Potrebić, Milica
AU  - Pešić, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5830
AB  - Parkinson's disease (PD) is characterized by both motor and non-motor symptoms.
Current research indicates that neuroinflammation, along with activated glial cells,
represents one of the key factors in the pathogenesis of PD. The aim of this study was
to assess non-motor symptoms of PD as well as the expression of gene markers of
microgliosis, astrogliosis and neuroinflammation in a transgenic A53T mouse model,
which is recommended for studying PD and other synucleinopathies. These transgenic
mice express an A53T missense mutant form of human alpha-synuclein under the
control of the murine prion promoter.
Depression-like symptoms and anhedonia were assessed using Tail Suspension Test
and Sucrose Preference Test respectively, while memory deficits were assessed using
Novel Object Recognition Test along with Object Relocation Test. Gene expression of
ionized calcium-binding adapter molecule (Iba-1), glial fibrillary acidic protein
(GFAP α and δ form) and cytokines associated with the microglial pro-inflammatory
phenotype (TNFα, IL-1β, IL-6) was assessed using RT-PCR. Brain regions of interest
were the prefrontal cortex, hippocampus, and striatum of 6 months old A53T male
mice. Non-transgenic littermates were used as controls. Obtained results suggest that
A53T mice compared to non-transgenic littermates exert 1) the presence of memory
deficits and anhedonia, 2) no difference in the expression of glial activity markers and
pro-inflammatory cytokines in the cortex and striatum, and 3) increased expression of
glial activity markers in the hippocampus, but without significant changes in the
expression of cytokines. Overall, better understanding of the role of active glia in the
model is needed.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Upregulation of glial markers with absence of a typical proinflammatory profile in the hippocampus of A53T mice as a model of Parkinson’s disease
SP  - 109
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5830
ER  - 

@conference{
author = "Dubljević, Olga and Pavković, Željko and Srbovan, Maja and Potrebić, Milica and Pešić, Vesna",
year = "2023",
abstract = "Parkinson's disease (PD) is characterized by both motor and non-motor symptoms.
Current research indicates that neuroinflammation, along with activated glial cells,
represents one of the key factors in the pathogenesis of PD. The aim of this study was
to assess non-motor symptoms of PD as well as the expression of gene markers of
microgliosis, astrogliosis and neuroinflammation in a transgenic A53T mouse model,
which is recommended for studying PD and other synucleinopathies. These transgenic
mice express an A53T missense mutant form of human alpha-synuclein under the
control of the murine prion promoter.
Depression-like symptoms and anhedonia were assessed using Tail Suspension Test
and Sucrose Preference Test respectively, while memory deficits were assessed using
Novel Object Recognition Test along with Object Relocation Test. Gene expression of
ionized calcium-binding adapter molecule (Iba-1), glial fibrillary acidic protein
(GFAP α and δ form) and cytokines associated with the microglial pro-inflammatory
phenotype (TNFα, IL-1β, IL-6) was assessed using RT-PCR. Brain regions of interest
were the prefrontal cortex, hippocampus, and striatum of 6 months old A53T male
mice. Non-transgenic littermates were used as controls. Obtained results suggest that
A53T mice compared to non-transgenic littermates exert 1) the presence of memory
deficits and anhedonia, 2) no difference in the expression of glial activity markers and
pro-inflammatory cytokines in the cortex and striatum, and 3) increased expression of
glial activity markers in the hippocampus, but without significant changes in the
expression of cytokines. Overall, better understanding of the role of active glia in the
model is needed.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Upregulation of glial markers with absence of a typical proinflammatory profile in the hippocampus of A53T mice as a model of Parkinson’s disease",
pages = "109",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5830"
}

Dubljević, O., Pavković, Ž., Srbovan, M., Potrebić, M.,& Pešić, V.. (2023). Upregulation of glial markers with absence of a typical proinflammatory profile in the hippocampus of A53T mice as a model of Parkinson’s disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 109.
https://hdl.handle.net/21.15107/rcub_ibiss_5830

Dubljević O, Pavković Ž, Srbovan M, Potrebić M, Pešić V. Upregulation of glial markers with absence of a typical proinflammatory profile in the hippocampus of A53T mice as a model of Parkinson’s disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:109.
https://hdl.handle.net/21.15107/rcub_ibiss_5830 .

Dubljević, Olga, Pavković, Željko, Srbovan, Maja, Potrebić, Milica, Pešić, Vesna, "Upregulation of glial markers with absence of a typical proinflammatory profile in the hippocampus of A53T mice as a model of Parkinson’s disease" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):109,
https://hdl.handle.net/21.15107/rcub_ibiss_5830 .

TY  - JOUR
AU  - Potrebić, Milica
AU  - Pavković, Željko
AU  - Srbovan, Maja
AU  - Đmura, Goran
AU  - Pešić, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5336
AB  - Changes in housing density, including individual housing, are commonly necessary in animal research. Obtaining reproducibility and translational validity in biomedical research requires an understanding of how animals adapt to changes in housing density. Existing literature mainly addresses acclimatization after transportation. We used a within-subject design to examine changes in behavior and weight gain of 4-mo-old male Wistar Han rats after reduction of their social group (RSG; due to removal of one rat from a cage containing 3 rats) and social isolation (SI; the removed rat) for the subsequent 2 wk. Changes in weight gain and in exploratory and center-avoidance behavior in an inescapable open arena (OA) were measured before (D0) and on days 7 and 14 (D7 and D14, respectively) after social change. The motor response to d-amphetamine (1.5 mg/kg), which stimulates behavioral arousal in response to novelty, was assessed at D14. Within-subject design revealed that RSG rats in OA had less locomotion at D7 but not more center-avoidance behavior and had returned to the D0 activity level at D14; SI rats in OA had consistently less locomotion and more center-avoidance behavior. Rearing behavior during OA exposure did not change in either group. However, SI rats showed more center-avoidance behavior in OA, greater weight gain, and less amphetamine-induced rearing at D14 as compared with RSG rats. These data indicate that after RSG, mature adult male rats require 2 wk to return to their baseline level of OA-related behavior, while after SI they gain weight and acquire maladaptive exploratory and center-avoidance behavior. The finding that SI produces maladaptive behavioral and physiologic alterations in adult male rats deserves attention because these changes could have confounding effects on research findings.
PB  - Memphis: American Association for Laboratory Animal Science
T2  - Journal of the American Association for Laboratory Animal Science
T1  - Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation
IS  - 6
VL  - 61
DO  - 10.30802/AALAS-JAALAS-22-000032
SP  - 615
EP  - 623
ER  - 

@article{
author = "Potrebić, Milica and Pavković, Željko and Srbovan, Maja and Đmura, Goran and Pešić, Vesna",
year = "2022",
abstract = "Changes in housing density, including individual housing, are commonly necessary in animal research. Obtaining reproducibility and translational validity in biomedical research requires an understanding of how animals adapt to changes in housing density. Existing literature mainly addresses acclimatization after transportation. We used a within-subject design to examine changes in behavior and weight gain of 4-mo-old male Wistar Han rats after reduction of their social group (RSG; due to removal of one rat from a cage containing 3 rats) and social isolation (SI; the removed rat) for the subsequent 2 wk. Changes in weight gain and in exploratory and center-avoidance behavior in an inescapable open arena (OA) were measured before (D0) and on days 7 and 14 (D7 and D14, respectively) after social change. The motor response to d-amphetamine (1.5 mg/kg), which stimulates behavioral arousal in response to novelty, was assessed at D14. Within-subject design revealed that RSG rats in OA had less locomotion at D7 but not more center-avoidance behavior and had returned to the D0 activity level at D14; SI rats in OA had consistently less locomotion and more center-avoidance behavior. Rearing behavior during OA exposure did not change in either group. However, SI rats showed more center-avoidance behavior in OA, greater weight gain, and less amphetamine-induced rearing at D14 as compared with RSG rats. These data indicate that after RSG, mature adult male rats require 2 wk to return to their baseline level of OA-related behavior, while after SI they gain weight and acquire maladaptive exploratory and center-avoidance behavior. The finding that SI produces maladaptive behavioral and physiologic alterations in adult male rats deserves attention because these changes could have confounding effects on research findings.",
publisher = "Memphis: American Association for Laboratory Animal Science",
journal = "Journal of the American Association for Laboratory Animal Science",
title = "Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation",
number = "6",
volume = "61",
doi = "10.30802/AALAS-JAALAS-22-000032",
pages = "615-623"
}

Potrebić, M., Pavković, Ž., Srbovan, M., Đmura, G.,& Pešić, V.. (2022). Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation. in Journal of the American Association for Laboratory Animal Science
Memphis: American Association for Laboratory Animal Science., 61(6), 615-623.
https://doi.org/10.30802/AALAS-JAALAS-22-000032

Potrebić M, Pavković Ž, Srbovan M, Đmura G, Pešić V. Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation. in Journal of the American Association for Laboratory Animal Science. 2022;61(6):615-623.
doi:10.30802/AALAS-JAALAS-22-000032 .

Potrebić, Milica, Pavković, Željko, Srbovan, Maja, Đmura, Goran, Pešić, Vesna, "Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation" in Journal of the American Association for Laboratory Animal Science, 61, no. 6 (2022):615-623,
https://doi.org/10.30802/AALAS-JAALAS-22-000032 . .

TY  - JOUR
AU  - Potrebić, Milica
AU  - Pavković, Željko
AU  - Puškaš, Nela
AU  - Pešić, Vesna
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fnbeh.2022.872628/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9113078
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4988
AB  - The fulfillment of belonging needs underlies a variety of behaviors. In order to understand how social needs unmet during maturation shape everyday life, we examined social motivation and cognition in peripubertal rats, as a rodent model of adolescence, subjected to social isolation (SI) during early and early-to-mid adolescence. The behavioral correlates of social orientation (social space preference), sociability (preference for social over non-social novelty), and social novelty preference (SNP) were examined in group-housed (GH) and single-housed (SH) rats in a 3-chamber test. The response to social odors was examined to gain insights into the developmental role of social odors in motivated social behavior. Differentiation between appetitive (number of visits/approaches) and consummatory (exploratory time) aspects of motivated social behavior was done to determine which facet of social motivation characterizes maturation when social needs are met and which aspect dominates when social needs are unsatisfied. The SI-sensitive parvalbumin-expressing interneurons (PVI) in the hippocampus were examined using immunohistochemistry. The main findings are the following: (1) in GH rats, the preference for social space is not evident regardless of animals' age, while sociability becomes apparent in mid-adolescence strictly through consummatory behavior, along with complete SNP (appetitive, consummatory); (2) SH promotes staying in a social chamber/space regardless of animals' age and produces an appetitive preference for it only in early-adolescent animals; (3) SH promotes sociability (appetitive, consummatory) regardless of the animals' age and prevents the SNP; (4) the preference for a social odor is displayed in all the groups through consummatory behavior, while appetitive behavior is evident only in SH rats; (5) the response to social odors does not commensurate directly to the response to conspecifics; (6) SH does not influence PVI in the hippocampus, except in the case of early-adolescence when a transient decrease in the dentate gyrus is observed. These results accentuate the developmental complexity of social motivation and cognition, and the power of SI in adolescence to infringe social maturation at different functional levels, promoting appetitive behavior toward peers overall but harming the interest for social novelty. The findings emphasize the importance of the fulfillment of basic social needs in the navigation through the social world.
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Behavioral Neuroscience
T1  - The Influence of Social Isolation on Social Orientation, Sociability, Social Novelty Preference, and Hippocampal Parvalbumin-Expressing Interneurons in Peripubertal Rats - Understanding the Importance of Meeting Social Needs in Adolescence.
VL  - 16
DO  - 10.3389/fnbeh.2022.872628
SP  - 872628
ER  - 

@article{
author = "Potrebić, Milica and Pavković, Željko and Puškaš, Nela and Pešić, Vesna",
year = "2022",
abstract = "The fulfillment of belonging needs underlies a variety of behaviors. In order to understand how social needs unmet during maturation shape everyday life, we examined social motivation and cognition in peripubertal rats, as a rodent model of adolescence, subjected to social isolation (SI) during early and early-to-mid adolescence. The behavioral correlates of social orientation (social space preference), sociability (preference for social over non-social novelty), and social novelty preference (SNP) were examined in group-housed (GH) and single-housed (SH) rats in a 3-chamber test. The response to social odors was examined to gain insights into the developmental role of social odors in motivated social behavior. Differentiation between appetitive (number of visits/approaches) and consummatory (exploratory time) aspects of motivated social behavior was done to determine which facet of social motivation characterizes maturation when social needs are met and which aspect dominates when social needs are unsatisfied. The SI-sensitive parvalbumin-expressing interneurons (PVI) in the hippocampus were examined using immunohistochemistry. The main findings are the following: (1) in GH rats, the preference for social space is not evident regardless of animals' age, while sociability becomes apparent in mid-adolescence strictly through consummatory behavior, along with complete SNP (appetitive, consummatory); (2) SH promotes staying in a social chamber/space regardless of animals' age and produces an appetitive preference for it only in early-adolescent animals; (3) SH promotes sociability (appetitive, consummatory) regardless of the animals' age and prevents the SNP; (4) the preference for a social odor is displayed in all the groups through consummatory behavior, while appetitive behavior is evident only in SH rats; (5) the response to social odors does not commensurate directly to the response to conspecifics; (6) SH does not influence PVI in the hippocampus, except in the case of early-adolescence when a transient decrease in the dentate gyrus is observed. These results accentuate the developmental complexity of social motivation and cognition, and the power of SI in adolescence to infringe social maturation at different functional levels, promoting appetitive behavior toward peers overall but harming the interest for social novelty. The findings emphasize the importance of the fulfillment of basic social needs in the navigation through the social world.",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Behavioral Neuroscience",
title = "The Influence of Social Isolation on Social Orientation, Sociability, Social Novelty Preference, and Hippocampal Parvalbumin-Expressing Interneurons in Peripubertal Rats - Understanding the Importance of Meeting Social Needs in Adolescence.",
volume = "16",
doi = "10.3389/fnbeh.2022.872628",
pages = "872628"
}

Potrebić, M., Pavković, Ž., Puškaš, N.,& Pešić, V.. (2022). The Influence of Social Isolation on Social Orientation, Sociability, Social Novelty Preference, and Hippocampal Parvalbumin-Expressing Interneurons in Peripubertal Rats - Understanding the Importance of Meeting Social Needs in Adolescence.. in Frontiers in Behavioral Neuroscience
Lausanne: Frontiers Media S.A.., 16, 872628.
https://doi.org/10.3389/fnbeh.2022.872628

Potrebić M, Pavković Ž, Puškaš N, Pešić V. The Influence of Social Isolation on Social Orientation, Sociability, Social Novelty Preference, and Hippocampal Parvalbumin-Expressing Interneurons in Peripubertal Rats - Understanding the Importance of Meeting Social Needs in Adolescence.. in Frontiers in Behavioral Neuroscience. 2022;16:872628.
doi:10.3389/fnbeh.2022.872628 .

Potrebić, Milica, Pavković, Željko, Puškaš, Nela, Pešić, Vesna, "The Influence of Social Isolation on Social Orientation, Sociability, Social Novelty Preference, and Hippocampal Parvalbumin-Expressing Interneurons in Peripubertal Rats - Understanding the Importance of Meeting Social Needs in Adolescence." in Frontiers in Behavioral Neuroscience, 16 (2022):872628,
https://doi.org/10.3389/fnbeh.2022.872628 . .

TY  - CONF
AU  - Pavković, Željko
AU  - Potrebić, Milica
AU  - Dubljević, Olga
AU  - Srbovan, Maja
AU  - Pešić, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5614
AB  - Познато је да су анксиозни поремећаји генерално изражени током адолесценције док специфичности везане за интензитет симптома током различитих фаза адолесценције (рана, средња, касна) још увек нису довољно проучене. Aдолесценција је као период матурације присутна код свих сисара, што оправдава коришћење анималних модела у психобиологији. Имајући у виду дефинисане фазе адолесценције глодара, испитивали смо матурацију експлораторног и анксиозног одговора на новину код мужјака Wistar Han пацова старих 35 ± 1 дан (крај ране/почетак средње адолесценције), 49 ± 1 дан (крај средње/почетак касне адолесценције) и 155 ± 1 дан (адултно доба). Коришћени су тестови у којима животиња слободно истражује нову средину (нова правоугаона арена, црно-бела кутија, уздигнути крстасти лавиринт). Адаптабилност на нову средину је процењивана поређењем бихејвиоралних одговора у првих и последњих 5 минута тестирања. Добијени резултати су показали да код пацова 1) матурациона крива експлораторног одговора на нову средину може имати како линеарни тако и U-профил, у зависности од карактеристика новe средине, 2) понашање налик анксиозном има инвертовани U-профил, док се 3) прилагодљивост независно од срединских услова јавља крајем средње/почетком касне адолесценције. Дискутован је потенцијални значај добијених резултата.
AB  - Poznato je da su anksiozni poremećaji generalno izraženi tokom adolescencije dok specifičnosti vezane za intenzitet simptoma tokom različitih faza adolescencije (rana, srednja, kasna) još uvek nisu dovoljno proučene. Adolescencija je kao period maturacije prisutna kod svih sisara, što opravdava korišćenje animalnih modela u psihobiologiji. Imajući u vidu definisane faze adolescencije glodara, ispitivali smo maturaciju eksploratornog i anksioznog odgovora na novinu kod mužjaka Wistar Han pacova starih 35 ± 1 dan (kraj rane/početak srednje adolescencije), 49 ± 1 dan (kraj srednje/početak kasne adolescencije) i 155 ± 1 dan (adultno doba). Korišćeni su testovi u kojima životinja slobodno istražuje novu sredinu (nova pravougaona arena, crno-bela kutija, uzdignuti krstasti lavirint). Adaptabilnost na novu sredinu je procenjivana poređenjem bihejvioralnih odgovora u prvih i poslednjih 5 minuta testiranja. Dobijeni rezultati su pokazali da kod pacova 1) maturaciona kriva eksploratornog odgovora na novu sredinu može imati kako linearni tako i U-profil, u zavisnosti od karakteristika nove sredine, 2) ponašanje nalik anksioznom ima invertovani U-profil, dok se 3) prilagodljivost nezavisno od sredinskih uslova javlja krajem srednje/početkom kasne adolescencije. Diskutovan je potencijalni značaj dobijenih rezultata.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Матурација експлораторног, анксиозног и адаптивног одговора на нове средине у моделу пацова
T1  - Maturacija eksploratornog, anksioznog i adaptivnog odgovora na nove sredine u modelu pacova
SP  - 339
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5614
ER  - 

@conference{
author = "Pavković, Željko and Potrebić, Milica and Dubljević, Olga and Srbovan, Maja and Pešić, Vesna",
year = "2022",
abstract = "Познато је да су анксиозни поремећаји генерално изражени током адолесценције док специфичности везане за интензитет симптома током различитих фаза адолесценције (рана, средња, касна) још увек нису довољно проучене. Aдолесценција је као период матурације присутна код свих сисара, што оправдава коришћење анималних модела у психобиологији. Имајући у виду дефинисане фазе адолесценције глодара, испитивали смо матурацију експлораторног и анксиозног одговора на новину код мужјака Wistar Han пацова старих 35 ± 1 дан (крај ране/почетак средње адолесценције), 49 ± 1 дан (крај средње/почетак касне адолесценције) и 155 ± 1 дан (адултно доба). Коришћени су тестови у којима животиња слободно истражује нову средину (нова правоугаона арена, црно-бела кутија, уздигнути крстасти лавиринт). Адаптабилност на нову средину је процењивана поређењем бихејвиоралних одговора у првих и последњих 5 минута тестирања. Добијени резултати су показали да код пацова 1) матурациона крива експлораторног одговора на нову средину може имати како линеарни тако и U-профил, у зависности од карактеристика новe средине, 2) понашање налик анксиозном има инвертовани U-профил, док се 3) прилагодљивост независно од срединских услова јавља крајем средње/почетком касне адолесценције. Дискутован је потенцијални значај добијених резултата., Poznato je da su anksiozni poremećaji generalno izraženi tokom adolescencije dok specifičnosti vezane za intenzitet simptoma tokom različitih faza adolescencije (rana, srednja, kasna) još uvek nisu dovoljno proučene. Adolescencija je kao period maturacije prisutna kod svih sisara, što opravdava korišćenje animalnih modela u psihobiologiji. Imajući u vidu definisane faze adolescencije glodara, ispitivali smo maturaciju eksploratornog i anksioznog odgovora na novinu kod mužjaka Wistar Han pacova starih 35 ± 1 dan (kraj rane/početak srednje adolescencije), 49 ± 1 dan (kraj srednje/početak kasne adolescencije) i 155 ± 1 dan (adultno doba). Korišćeni su testovi u kojima životinja slobodno istražuje novu sredinu (nova pravougaona arena, crno-bela kutija, uzdignuti krstasti lavirint). Adaptabilnost na novu sredinu je procenjivana poređenjem bihejvioralnih odgovora u prvih i poslednjih 5 minuta testiranja. Dobijeni rezultati su pokazali da kod pacova 1) maturaciona kriva eksploratornog odgovora na novu sredinu može imati kako linearni tako i U-profil, u zavisnosti od karakteristika nove sredine, 2) ponašanje nalik anksioznom ima invertovani U-profil, dok se 3) prilagodljivost nezavisno od sredinskih uslova javlja krajem srednje/početkom kasne adolescencije. Diskutovan je potencijalni značaj dobijenih rezultata.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Матурација експлораторног, анксиозног и адаптивног одговора на нове средине у моделу пацова, Maturacija eksploratornog, anksioznog i adaptivnog odgovora na nove sredine u modelu pacova",
pages = "339",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5614"
}

Pavković, Ž., Potrebić, M., Dubljević, O., Srbovan, M.,& Pešić, V.. (2022). Матурација експлораторног, анксиозног и адаптивног одговора на нове средине у моделу пацова. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 339.
https://hdl.handle.net/21.15107/rcub_ibiss_5614

Pavković Ž, Potrebić M, Dubljević O, Srbovan M, Pešić V. Матурација експлораторног, анксиозног и адаптивног одговора на нове средине у моделу пацова. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:339.
https://hdl.handle.net/21.15107/rcub_ibiss_5614 .

Pavković, Željko, Potrebić, Milica, Dubljević, Olga, Srbovan, Maja, Pešić, Vesna, "Матурација експлораторног, анксиозног и адаптивног одговора на нове средине у моделу пацова" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):339,
https://hdl.handle.net/21.15107/rcub_ibiss_5614 .

TY  - CONF
AU  - Potrebić, Milica
AU  - Pavković, Željko
AU  - Pešić, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5609
AB  - The influence of parental social experience on the offspring neurodevelopment and its own susceptibility to the sculpting effect of social experience are not well understood. Using rat model we aimed to examine whether and how social experience of adolescent parents impacts psychophysical characteristics of their offspring, during pre-weaning period and later during adolescent growing in defined social conditions. 
Methods: Peripubertal Wistar rats (both sexes) were randomly selected for group housing (GH; n=3 per cage) or single housing (SH; n=1 per cage) at postnatal day (P) 29 and left undisturbed until P55. Thereafter, they were grouped for mating (SH males with SH females, GH males with GH females). Body weight (BW) of pups was monitored at P7, P14, and P21. At P17 motor activity of the offspring was monitored; at P29 the animals were subjected to GH or SH; after 1 week (P36) and 2 weeks (P43) their motor activity was monitored; at P45 they were subjected to sucrose preference test (SPT). 
Results: The male offspring of SH parents had decreased locomotor activity in a novel open arena, at P17. At P36 and P43, adolescent SH offspring showed hypolocomotion in a novel arena and hypohedonic behavior in SPT compared to GH counterparts, regardless of parental experience. BW did not differ between groups. 
Conclusions: Parental social experience influences novelty-exploring behavior of offspring, producing response below expected at P17. With further offspring development, their individual social experience has stronger impact on the appearance of depression-like phenotypes than parental social experience.
PB  - Federation of European Neuroscience Societies
C3  - E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France
T1  - The influence of parental social experience of offspring novelty-exploring and drepression-like behavior
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5609
ER  - 

@conference{
author = "Potrebić, Milica and Pavković, Željko and Pešić, Vesna",
year = "2022",
abstract = "The influence of parental social experience on the offspring neurodevelopment and its own susceptibility to the sculpting effect of social experience are not well understood. Using rat model we aimed to examine whether and how social experience of adolescent parents impacts psychophysical characteristics of their offspring, during pre-weaning period and later during adolescent growing in defined social conditions. 
Methods: Peripubertal Wistar rats (both sexes) were randomly selected for group housing (GH; n=3 per cage) or single housing (SH; n=1 per cage) at postnatal day (P) 29 and left undisturbed until P55. Thereafter, they were grouped for mating (SH males with SH females, GH males with GH females). Body weight (BW) of pups was monitored at P7, P14, and P21. At P17 motor activity of the offspring was monitored; at P29 the animals were subjected to GH or SH; after 1 week (P36) and 2 weeks (P43) their motor activity was monitored; at P45 they were subjected to sucrose preference test (SPT). 
Results: The male offspring of SH parents had decreased locomotor activity in a novel open arena, at P17. At P36 and P43, adolescent SH offspring showed hypolocomotion in a novel arena and hypohedonic behavior in SPT compared to GH counterparts, regardless of parental experience. BW did not differ between groups. 
Conclusions: Parental social experience influences novelty-exploring behavior of offspring, producing response below expected at P17. With further offspring development, their individual social experience has stronger impact on the appearance of depression-like phenotypes than parental social experience.",
publisher = "Federation of European Neuroscience Societies",
journal = "E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France",
title = "The influence of parental social experience of offspring novelty-exploring and drepression-like behavior",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5609"
}

Potrebić, M., Pavković, Ž.,& Pešić, V.. (2022). The influence of parental social experience of offspring novelty-exploring and drepression-like behavior. in E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France
Federation of European Neuroscience Societies..
https://hdl.handle.net/21.15107/rcub_ibiss_5609

Potrebić M, Pavković Ž, Pešić V. The influence of parental social experience of offspring novelty-exploring and drepression-like behavior. in E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5609 .

Potrebić, Milica, Pavković, Željko, Pešić, Vesna, "The influence of parental social experience of offspring novelty-exploring and drepression-like behavior" in E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5609 .

TY  - JOUR
AU  - Potrebić, Milica
AU  - Pavković, Željko
AU  - Lončarević-Vasiljković, Nataša
AU  - Kanazir, Selma
AU  - Pešić, Vesna
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4073
AB  - Reduction in direct social contact with peers during early adolescence is thought to be a risk factor for an increase in depressive symptoms, but there is still no clear evidence to suggest early behavioral manifestations and their association with the later outcome of social distancing during this period. To address this question, we used social isolation paradigm in peripubertal rats as the rodent model of adolescence. The litter was an experimental unit. On postnatal day 29, each litter gave group-housed and single-housed males, which were reared and tested one week and two weeks thereafter. Psychomotor/emotional response to novelty in exploration-based tasks, behavioral and neuronal responses to the drug reward (D-amphetamine), motivation/hedonic behavior, physiological and response to physiological stress were examined. Social isolation in peripubertal rats manifested through: hyper-reactivity/agitation and the state anxiety/risk-taking at an early stage; reduced behavioral response to D-amphetamine and altered neural processing of this stimulus, at a later stage; consummatory hypohedonia that deepened over time without changing the motivation to eat; unchanged body weight gain and resting blood corticosterone, cortisol and glucose levels over time; altered blood biochemistry (silenced corticosterone and increased glucose) due to overnight fasting only at an early stage. Our results highlight that the outcome of reduced direct social contact with peers during peripuberty is dynamic, with the cluster of atypical early symptoms that evolve into the syndrome that is delicate for assessment through routinely measurable behavior and biomarkers of stress, but with progressive consummatory hypohedonia and unaffected motivation to eat as stable marks.
PB  - Elsevier Inc.
T2  - Progress in Neuro-Psychopharmacology and Biological Psychiatry
T1  - Altered hedonic, novelty-, stress- and D-amphetamine-induced response to due to social isolation in peripuberty
VL  - 108
DO  - 10.1016/j.pnpbp.2020.110186
SP  - 110186
ER  - 

@article{
author = "Potrebić, Milica and Pavković, Željko and Lončarević-Vasiljković, Nataša and Kanazir, Selma and Pešić, Vesna",
year = "2021",
abstract = "Reduction in direct social contact with peers during early adolescence is thought to be a risk factor for an increase in depressive symptoms, but there is still no clear evidence to suggest early behavioral manifestations and their association with the later outcome of social distancing during this period. To address this question, we used social isolation paradigm in peripubertal rats as the rodent model of adolescence. The litter was an experimental unit. On postnatal day 29, each litter gave group-housed and single-housed males, which were reared and tested one week and two weeks thereafter. Psychomotor/emotional response to novelty in exploration-based tasks, behavioral and neuronal responses to the drug reward (D-amphetamine), motivation/hedonic behavior, physiological and response to physiological stress were examined. Social isolation in peripubertal rats manifested through: hyper-reactivity/agitation and the state anxiety/risk-taking at an early stage; reduced behavioral response to D-amphetamine and altered neural processing of this stimulus, at a later stage; consummatory hypohedonia that deepened over time without changing the motivation to eat; unchanged body weight gain and resting blood corticosterone, cortisol and glucose levels over time; altered blood biochemistry (silenced corticosterone and increased glucose) due to overnight fasting only at an early stage. Our results highlight that the outcome of reduced direct social contact with peers during peripuberty is dynamic, with the cluster of atypical early symptoms that evolve into the syndrome that is delicate for assessment through routinely measurable behavior and biomarkers of stress, but with progressive consummatory hypohedonia and unaffected motivation to eat as stable marks.",
publisher = "Elsevier Inc.",
journal = "Progress in Neuro-Psychopharmacology and Biological Psychiatry",
title = "Altered hedonic, novelty-, stress- and D-amphetamine-induced response to due to social isolation in peripuberty",
volume = "108",
doi = "10.1016/j.pnpbp.2020.110186",
pages = "110186"
}

Potrebić, M., Pavković, Ž., Lončarević-Vasiljković, N., Kanazir, S.,& Pešić, V.. (2021). Altered hedonic, novelty-, stress- and D-amphetamine-induced response to due to social isolation in peripuberty. in Progress in Neuro-Psychopharmacology and Biological Psychiatry
Elsevier Inc.., 108, 110186.
https://doi.org/10.1016/j.pnpbp.2020.110186

Potrebić M, Pavković Ž, Lončarević-Vasiljković N, Kanazir S, Pešić V. Altered hedonic, novelty-, stress- and D-amphetamine-induced response to due to social isolation in peripuberty. in Progress in Neuro-Psychopharmacology and Biological Psychiatry. 2021;108:110186.
doi:10.1016/j.pnpbp.2020.110186 .

Potrebić, Milica, Pavković, Željko, Lončarević-Vasiljković, Nataša, Kanazir, Selma, Pešić, Vesna, "Altered hedonic, novelty-, stress- and D-amphetamine-induced response to due to social isolation in peripuberty" in Progress in Neuro-Psychopharmacology and Biological Psychiatry, 108 (2021):110186,
https://doi.org/10.1016/j.pnpbp.2020.110186 . .

TY  - JOUR
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Jarić, Ivana
AU  - Šošić-Jurjević, Branka
AU  - Potrebić, Milica
AU  - Milošević, Verica
PY  - 2019
UR  - http://online.journals.tubitak.gov.tr/openDoiPdf.htm?mKodu=vet-1812-39
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3402
AB  - Letter to the Editor
T2  - Turkish Journal of Veterinary and Animal Sciences
T1  - Old age-associated impairment of the rat liver antioxidant defense system: the basis for affirmation of the experimental model
IS  - 3
VL  - 43
DO  - 10.3906/vet-1812-39
SP  - 423
EP  - 426
ER  - 

@article{
author = "Miler, Marko and Ajdžanović, Vladimir and Živanović, Jasmina and Jarić, Ivana and Šošić-Jurjević, Branka and Potrebić, Milica and Milošević, Verica",
year = "2019",
abstract = "Letter to the Editor",
journal = "Turkish Journal of Veterinary and Animal Sciences",
title = "Old age-associated impairment of the rat liver antioxidant defense system: the basis for affirmation of the experimental model",
number = "3",
volume = "43",
doi = "10.3906/vet-1812-39",
pages = "423-426"
}

Miler, M., Ajdžanović, V., Živanović, J., Jarić, I., Šošić-Jurjević, B., Potrebić, M.,& Milošević, V.. (2019). Old age-associated impairment of the rat liver antioxidant defense system: the basis for affirmation of the experimental model. in Turkish Journal of Veterinary and Animal Sciences, 43(3), 423-426.
https://doi.org/10.3906/vet-1812-39

Miler M, Ajdžanović V, Živanović J, Jarić I, Šošić-Jurjević B, Potrebić M, Milošević V. Old age-associated impairment of the rat liver antioxidant defense system: the basis for affirmation of the experimental model. in Turkish Journal of Veterinary and Animal Sciences. 2019;43(3):423-426.
doi:10.3906/vet-1812-39 .

Miler, Marko, Ajdžanović, Vladimir, Živanović, Jasmina, Jarić, Ivana, Šošić-Jurjević, Branka, Potrebić, Milica, Milošević, Verica, "Old age-associated impairment of the rat liver antioxidant defense system: the basis for affirmation of the experimental model" in Turkish Journal of Veterinary and Animal Sciences, 43, no. 3 (2019):423-426,
https://doi.org/10.3906/vet-1812-39 . .

TY  - JOUR
AU  - Pavković, Željko
AU  - Potrebić, Milica
AU  - Kanazir, Selma
AU  - Pešić, Vesna
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0278584619304245?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3455
AB  - Adolescent neurodevelopment confer vulnerability to the actions of treatments that produce adaptations in neurocircuitry underlying motivation, impulsivity and reward. Considering wide usage of a sedative-hypnotic agent propofol in clinical practice, we examined whether propofol is a challenging treatment for peripubertal brain. Motivation/hedonic behavior (sucrose preference test), approach/avoidance behavior (elevated plus maze test) and response to dissociative drug phencyclidine (PCP) were studied in peripubertal rats (the rodent model of periadolescence) after propofol anesthesia exposure (PAE). Neurodegeneration (Fluoro-Jade staining) and the expression of proteins (Western blot) involved in excitatory synaptic transmission and activity-dependent synaptic stabilization in the medial prefrontal cortex (mPFC) and striatum (components of motivation/reward circuitry; process both appetitive and aversive events) were examined as well. In peripubertal rats PAE produced 1) transient brain-region specific changes in the expression of N-methyl-d-aspartate (NMDA) receptor subunits NR2A and NR2B, PSD-95 and N-cadherin, without neurotoxicity, 2) hyperlocomotor response to PCP, 3) no changes in preference for palatable 1% sucrose solution and a decrease in food eaten, 4) preference for 20% sucrose solution without changes in food eaten, 5) stretch-attended postures and open arms entries in the elevated plus maze test. Overall, these novel findings show that PAE leaves transient synaptic trace recognized as early form of synaptic plasticity related to passive drug exposure in the brain systems implicated in motivation/reward, increases drug-responsiveness, favors risk-taking and preference of novel/intense stimuli repairing otherwise present motivational deficiency. These findings accentuate multifaceted response to propofol in peripuberty and the importance of environmental stability for the most favorable neurobehavioral recovery.
T2  - Progress in Neuro-Psychopharmacology and Biological Psychiatry
T1  - Motivation, risk-taking and sensation seeking behavior in propofol anesthesia exposed peripubertal rats.
VL  - 96
DO  - 10.1016/j.pnpbp.2019.109733
SP  - 109733
ER  - 

@article{
author = "Pavković, Željko and Potrebić, Milica and Kanazir, Selma and Pešić, Vesna",
year = "2019",
abstract = "Adolescent neurodevelopment confer vulnerability to the actions of treatments that produce adaptations in neurocircuitry underlying motivation, impulsivity and reward. Considering wide usage of a sedative-hypnotic agent propofol in clinical practice, we examined whether propofol is a challenging treatment for peripubertal brain. Motivation/hedonic behavior (sucrose preference test), approach/avoidance behavior (elevated plus maze test) and response to dissociative drug phencyclidine (PCP) were studied in peripubertal rats (the rodent model of periadolescence) after propofol anesthesia exposure (PAE). Neurodegeneration (Fluoro-Jade staining) and the expression of proteins (Western blot) involved in excitatory synaptic transmission and activity-dependent synaptic stabilization in the medial prefrontal cortex (mPFC) and striatum (components of motivation/reward circuitry; process both appetitive and aversive events) were examined as well. In peripubertal rats PAE produced 1) transient brain-region specific changes in the expression of N-methyl-d-aspartate (NMDA) receptor subunits NR2A and NR2B, PSD-95 and N-cadherin, without neurotoxicity, 2) hyperlocomotor response to PCP, 3) no changes in preference for palatable 1% sucrose solution and a decrease in food eaten, 4) preference for 20% sucrose solution without changes in food eaten, 5) stretch-attended postures and open arms entries in the elevated plus maze test. Overall, these novel findings show that PAE leaves transient synaptic trace recognized as early form of synaptic plasticity related to passive drug exposure in the brain systems implicated in motivation/reward, increases drug-responsiveness, favors risk-taking and preference of novel/intense stimuli repairing otherwise present motivational deficiency. These findings accentuate multifaceted response to propofol in peripuberty and the importance of environmental stability for the most favorable neurobehavioral recovery.",
journal = "Progress in Neuro-Psychopharmacology and Biological Psychiatry",
title = "Motivation, risk-taking and sensation seeking behavior in propofol anesthesia exposed peripubertal rats.",
volume = "96",
doi = "10.1016/j.pnpbp.2019.109733",
pages = "109733"
}

Pavković, Ž., Potrebić, M., Kanazir, S.,& Pešić, V.. (2019). Motivation, risk-taking and sensation seeking behavior in propofol anesthesia exposed peripubertal rats.. in Progress in Neuro-Psychopharmacology and Biological Psychiatry, 96, 109733.
https://doi.org/10.1016/j.pnpbp.2019.109733

Pavković Ž, Potrebić M, Kanazir S, Pešić V. Motivation, risk-taking and sensation seeking behavior in propofol anesthesia exposed peripubertal rats.. in Progress in Neuro-Psychopharmacology and Biological Psychiatry. 2019;96:109733.
doi:10.1016/j.pnpbp.2019.109733 .

Pavković, Željko, Potrebić, Milica, Kanazir, Selma, Pešić, Vesna, "Motivation, risk-taking and sensation seeking behavior in propofol anesthesia exposed peripubertal rats." in Progress in Neuro-Psychopharmacology and Biological Psychiatry, 96 (2019):109733,
https://doi.org/10.1016/j.pnpbp.2019.109733 . .

TY  - CHAP
AU  - Miličić, Dragana
AU  - Savić, Tatjana
AU  - Petković, Branka
AU  - Potrebić, Milica
AU  - Trajković, Jelena
AU  - Pavković-Lučić, Sofija
PY  - 2019
UR  - https://novapublishers.com/shop/the-zoological-guide-to-crustacea/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3814
AB  - In aquatic environments, chemical cues are very important for the perception of danger, especially when visibility is low. It is known that ostracods rely on chemical senses to detect predators, which is essential for survival. Occurrence of alarm signals in the surroundings can affect their activities and behaviour. The present study investigates if the non-marine ostracod Heterocypris incongruens (Crustacea: Ostracoda) can detect and react to chemical compounds derived from a predator (Triturus spp. larvae) and from injured conspecifics. Also, the study aims to investigate whether habitat-substrate selection is at play when the individuals are in potential danger. The obtained results indicate that predator-derived chemicals and conspecific alarm cues induce specific behavioural responses: forming of aggregations, reduction of locomotion or camouflaging. It is possible that H. incongruens rapidly evaluates environmental cues and modifies defensive strategies depending on the type of semiochemicals perceived.
PB  - New York, USA: Nova Science Publishers, Inc.
T2  - The Zoological Guide to Crustacea
T1  - Behavioural Responses of the Non-Marine Ostracod Heterocypris incongruens (Crustacea: Ostracoda) to Natural Chemical Cues: Laboratory Observations
SP  - 89
EP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3814
ER  - 

@inbook{
author = "Miličić, Dragana and Savić, Tatjana and Petković, Branka and Potrebić, Milica and Trajković, Jelena and Pavković-Lučić, Sofija",
year = "2019",
abstract = "In aquatic environments, chemical cues are very important for the perception of danger, especially when visibility is low. It is known that ostracods rely on chemical senses to detect predators, which is essential for survival. Occurrence of alarm signals in the surroundings can affect their activities and behaviour. The present study investigates if the non-marine ostracod Heterocypris incongruens (Crustacea: Ostracoda) can detect and react to chemical compounds derived from a predator (Triturus spp. larvae) and from injured conspecifics. Also, the study aims to investigate whether habitat-substrate selection is at play when the individuals are in potential danger. The obtained results indicate that predator-derived chemicals and conspecific alarm cues induce specific behavioural responses: forming of aggregations, reduction of locomotion or camouflaging. It is possible that H. incongruens rapidly evaluates environmental cues and modifies defensive strategies depending on the type of semiochemicals perceived.",
publisher = "New York, USA: Nova Science Publishers, Inc.",
journal = "The Zoological Guide to Crustacea",
booktitle = "Behavioural Responses of the Non-Marine Ostracod Heterocypris incongruens (Crustacea: Ostracoda) to Natural Chemical Cues: Laboratory Observations",
pages = "89-112",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3814"
}

Miličić, D., Savić, T., Petković, B., Potrebić, M., Trajković, J.,& Pavković-Lučić, S.. (2019). Behavioural Responses of the Non-Marine Ostracod Heterocypris incongruens (Crustacea: Ostracoda) to Natural Chemical Cues: Laboratory Observations. in The Zoological Guide to Crustacea
New York, USA: Nova Science Publishers, Inc.., 89-112.
https://hdl.handle.net/21.15107/rcub_ibiss_3814

Miličić D, Savić T, Petković B, Potrebić M, Trajković J, Pavković-Lučić S. Behavioural Responses of the Non-Marine Ostracod Heterocypris incongruens (Crustacea: Ostracoda) to Natural Chemical Cues: Laboratory Observations. in The Zoological Guide to Crustacea. 2019;:89-112.
https://hdl.handle.net/21.15107/rcub_ibiss_3814 .

Miličić, Dragana, Savić, Tatjana, Petković, Branka, Potrebić, Milica, Trajković, Jelena, Pavković-Lučić, Sofija, "Behavioural Responses of the Non-Marine Ostracod Heterocypris incongruens (Crustacea: Ostracoda) to Natural Chemical Cues: Laboratory Observations" in The Zoological Guide to Crustacea (2019):89-112,
https://hdl.handle.net/21.15107/rcub_ibiss_3814 .

TY  - CONF
AU  - Potrebić, Milica
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Milenković, Dragan
AU  - Pendoski, Laze
AU  - Jakovljević, Vladimir
AU  - Milošević, Verica
PY  - 2018
UR  - http://www.enea2018.com/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6041
AB  - Objective: The complex process of aging can be defined as progressive loss of the ability to maintain
tissue homeostasis, which may cause many pathological issues, like depression, Alzheimer’s diseases
or cognitive deficits. Due to their various established health effects, citrus flavanones, naringenin
(NAR) and hesperetin (HES), could have important protective role against development and progression of many age-related diseases.The aim of this study was to examine the effects of these flavanones on pituitary-adrenocortical axis in the rat model of old age (24 months old Wistar rats), Methods: The experimental groups included control group, and naringenin and hesperetin treated groups. NAR and HES were administrated orally (15 mg/kg b. m.) during 4 weeks, while control group received sunflower oil as a vehicle. Concentrations of adrenocorticotropic (ACTH) hormone in pituitary ACTH cells (reflected by relative intensity of fluorescence) and blood levels of ACTH and corticosterone in experimental rats were determined semi-quantitatively and by using the immunoassays, respectively. Results: Results have shown that both blood concentrations of ACTH and corticosterone were markedly lower in NAR [51% and 39%, respectively (P<0.05)] and HES treated group [69% and 65%, respectively (P<0.05)] in comparison with the control group. The relative intensity of ACTH immunofluorescence signal was the highest in the control group, while in NAR and HES groups was significantly lower [39% and 65%, respectively (P<0.05)]. Conclusion: This study has shown that citrus flavanones affect pituitary-adrenocortical axis by decreasing ACTH levels in adrenocorticotropic cells and blood concentrations of ACTH and corticosterone, as well. Since the increased activation of this axis in elderly is associated with development of many aging-related neurophysiological issues, bioactive flavanones may be used in their prevention or delay.
PB  - Altdorf: EndoScience Endokrinologie Service GmbH
C3  - 18th Congress of the European Neuroendocrine Association: Abstracts; 2018 Oct 17-20; Wrocław, Poland
T1  - Citrus Flavanones, Naringenin and Hesperetin, decrease ACTH and Corticosterone Levels in Old Rats
SP  - 27
EP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6041
ER  - 

@conference{
author = "Potrebić, Milica and Ajdžanović, Vladimir and Miler, Marko and Milenković, Dragan and Pendoski, Laze and Jakovljević, Vladimir and Milošević, Verica",
year = "2018",
abstract = "Objective: The complex process of aging can be defined as progressive loss of the ability to maintain
tissue homeostasis, which may cause many pathological issues, like depression, Alzheimer’s diseases
or cognitive deficits. Due to their various established health effects, citrus flavanones, naringenin
(NAR) and hesperetin (HES), could have important protective role against development and progression of many age-related diseases.The aim of this study was to examine the effects of these flavanones on pituitary-adrenocortical axis in the rat model of old age (24 months old Wistar rats), Methods: The experimental groups included control group, and naringenin and hesperetin treated groups. NAR and HES were administrated orally (15 mg/kg b. m.) during 4 weeks, while control group received sunflower oil as a vehicle. Concentrations of adrenocorticotropic (ACTH) hormone in pituitary ACTH cells (reflected by relative intensity of fluorescence) and blood levels of ACTH and corticosterone in experimental rats were determined semi-quantitatively and by using the immunoassays, respectively. Results: Results have shown that both blood concentrations of ACTH and corticosterone were markedly lower in NAR [51% and 39%, respectively (P<0.05)] and HES treated group [69% and 65%, respectively (P<0.05)] in comparison with the control group. The relative intensity of ACTH immunofluorescence signal was the highest in the control group, while in NAR and HES groups was significantly lower [39% and 65%, respectively (P<0.05)]. Conclusion: This study has shown that citrus flavanones affect pituitary-adrenocortical axis by decreasing ACTH levels in adrenocorticotropic cells and blood concentrations of ACTH and corticosterone, as well. Since the increased activation of this axis in elderly is associated with development of many aging-related neurophysiological issues, bioactive flavanones may be used in their prevention or delay.",
publisher = "Altdorf: EndoScience Endokrinologie Service GmbH",
journal = "18th Congress of the European Neuroendocrine Association: Abstracts; 2018 Oct 17-20; Wrocław, Poland",
title = "Citrus Flavanones, Naringenin and Hesperetin, decrease ACTH and Corticosterone Levels in Old Rats",
pages = "27-28",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6041"
}

Potrebić, M., Ajdžanović, V., Miler, M., Milenković, D., Pendoski, L., Jakovljević, V.,& Milošević, V.. (2018). Citrus Flavanones, Naringenin and Hesperetin, decrease ACTH and Corticosterone Levels in Old Rats. in 18th Congress of the European Neuroendocrine Association: Abstracts; 2018 Oct 17-20; Wrocław, Poland
Altdorf: EndoScience Endokrinologie Service GmbH., 27-28.
https://hdl.handle.net/21.15107/rcub_ibiss_6041

Potrebić M, Ajdžanović V, Miler M, Milenković D, Pendoski L, Jakovljević V, Milošević V. Citrus Flavanones, Naringenin and Hesperetin, decrease ACTH and Corticosterone Levels in Old Rats. in 18th Congress of the European Neuroendocrine Association: Abstracts; 2018 Oct 17-20; Wrocław, Poland. 2018;:27-28.
https://hdl.handle.net/21.15107/rcub_ibiss_6041 .

Potrebić, Milica, Ajdžanović, Vladimir, Miler, Marko, Milenković, Dragan, Pendoski, Laze, Jakovljević, Vladimir, Milošević, Verica, "Citrus Flavanones, Naringenin and Hesperetin, decrease ACTH and Corticosterone Levels in Old Rats" in 18th Congress of the European Neuroendocrine Association: Abstracts; 2018 Oct 17-20; Wrocław, Poland (2018):27-28,
https://hdl.handle.net/21.15107/rcub_ibiss_6041 .

TY  - CONF
AU  - Ajdžanović, Vladimir
AU  - Jakovljević, Vladimir
AU  - Miler, Marko
AU  - Ristić, Nataša
AU  - Potrebić, Milica
AU  - Sretenović, Jasmina
AU  - Popovski Perčinić, Florina
AU  - Pendoski, Laze
AU  - Milošević, Verica
PY  - 2018
UR  - http://physiology.org.rs/4thCPS.php
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6035
AB  - The aim of this study was to investigate the immunohistomorphometric parameters of follicle-stimulating hormone (TSH) and luteinizing hormone (I.H) cells after testosterone application in an animal model of the andropause. Middle-aged Wistar rats were divided into orchidectomized (Or) and testosterone treated orchidectomized (OrxT) groups. Testosterone propionate (5 mg/kg b.m. (day) was administered for three weeks. while Or group received the vehicle alone.
Immunohistochemically stained FSH and I cells were subjected to the morphometric and optical density-related analysis. while circulating concentration of testosterone was measured by the immunoassay. Serum concentration of testosterone was increased (ps0.05) by 24 fold in comparison with Orx group.
Testosterone treatment did not cause significant changes of body mass or absolute and relative pituitary weights compared to Or group. The volume of FSH and LH cells was decreased (ps0.05) by 51.3% and 56.6% respectively, in comparison with Or rats. Relative volume density of FSI and LE cells was also decreased (p<0.05) by 54.0% and 72.8% respectively, compared to Or group. Results related to the optical density of gonadotropic cells were in line with the morphometric findings i.e. this parameter of FSH and LH cells was decreased (P«0.05) by 25.7% and 16.2% respectively, in comparison with Or rats. In conclusion. applied testosterone decreased morphometric parameters and optical density of gonadotropic cells in Or rats.
PB  - Belgrade : Serbian Physiological Society, 2018 (Lukovo : Galaksijanis)
C3  - Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia
T1  - Testosterone effects on the pituitary gonadotropic cells in an animal model of the andropause
SP  - 118
EP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6035
ER  - 

@conference{
author = "Ajdžanović, Vladimir and Jakovljević, Vladimir and Miler, Marko and Ristić, Nataša and Potrebić, Milica and Sretenović, Jasmina and Popovski Perčinić, Florina and Pendoski, Laze and Milošević, Verica",
year = "2018",
abstract = "The aim of this study was to investigate the immunohistomorphometric parameters of follicle-stimulating hormone (TSH) and luteinizing hormone (I.H) cells after testosterone application in an animal model of the andropause. Middle-aged Wistar rats were divided into orchidectomized (Or) and testosterone treated orchidectomized (OrxT) groups. Testosterone propionate (5 mg/kg b.m. (day) was administered for three weeks. while Or group received the vehicle alone.
Immunohistochemically stained FSH and I cells were subjected to the morphometric and optical density-related analysis. while circulating concentration of testosterone was measured by the immunoassay. Serum concentration of testosterone was increased (ps0.05) by 24 fold in comparison with Orx group.
Testosterone treatment did not cause significant changes of body mass or absolute and relative pituitary weights compared to Or group. The volume of FSH and LH cells was decreased (ps0.05) by 51.3% and 56.6% respectively, in comparison with Or rats. Relative volume density of FSI and LE cells was also decreased (p<0.05) by 54.0% and 72.8% respectively, compared to Or group. Results related to the optical density of gonadotropic cells were in line with the morphometric findings i.e. this parameter of FSH and LH cells was decreased (P«0.05) by 25.7% and 16.2% respectively, in comparison with Or rats. In conclusion. applied testosterone decreased morphometric parameters and optical density of gonadotropic cells in Or rats.",
publisher = "Belgrade : Serbian Physiological Society, 2018 (Lukovo : Galaksijanis)",
journal = "Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia",
title = "Testosterone effects on the pituitary gonadotropic cells in an animal model of the andropause",
pages = "118-118",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6035"
}

Ajdžanović, V., Jakovljević, V., Miler, M., Ristić, N., Potrebić, M., Sretenović, J., Popovski Perčinić, F., Pendoski, L.,& Milošević, V.. (2018). Testosterone effects on the pituitary gonadotropic cells in an animal model of the andropause. in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia
Belgrade : Serbian Physiological Society, 2018 (Lukovo : Galaksijanis)., 118-118.
https://hdl.handle.net/21.15107/rcub_ibiss_6035

Ajdžanović V, Jakovljević V, Miler M, Ristić N, Potrebić M, Sretenović J, Popovski Perčinić F, Pendoski L, Milošević V. Testosterone effects on the pituitary gonadotropic cells in an animal model of the andropause. in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia. 2018;:118-118.
https://hdl.handle.net/21.15107/rcub_ibiss_6035 .

Ajdžanović, Vladimir, Jakovljević, Vladimir, Miler, Marko, Ristić, Nataša, Potrebić, Milica, Sretenović, Jasmina, Popovski Perčinić, Florina, Pendoski, Laze, Milošević, Verica, "Testosterone effects on the pituitary gonadotropic cells in an animal model of the andropause" in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia (2018):118-118,
https://hdl.handle.net/21.15107/rcub_ibiss_6035 .

TY  - CONF
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Ajdžanović, Vladimir
AU  - Jarić, Ivana
AU  - Šošić-Jurjević, Branka
AU  - Marković, Zorica
AU  - Potrebić, Milica
AU  - Milošević, Verica
PY  - 2018
UR  - http://physiology.org.rs/4thCPS.php
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6024
AB  - Advanced age is associated with development of dyslipidemia, together with ectopic triglycerides (Tg) accumulation in the liver. Consumption of citrus flavanones, naringenin (NAR) and hesperetin (HES) positively correlates with improvement of dyslipidemia in rats. However, information about this effects of citrus flavanones in old-aged rats still lack. Therefor we analyzed changes in gene expressions of liver X receptor (Lxr), thyroid hormone responsive spot14 protein (Thrsp), fatty acid synthase (Fas) and fatty acid translocase (Fat) in the liver by qPCR and serum Tg concentration biochemically. NAR or HES, suspended in vehicle (sunflower oil) were administrated orally (15 mg/kg) to male 24-month-old Wistar rats (n=5) during 4 weeks. Control groups received vehicle (CON) or stayed physiologically intact (ICON). In comparison to ICON animals, only Fat gene increased after sunflower oil administration. NAR and HES treatment decreased mRNA expression of Thrsp, Lxr and Fat genes in comparison to CON. Only NAR decreased Fas gene expression as well, while Tg concentration remained unchanged after both. All changes were statistically significant (p<0.05). In conclusion, nutritional doses of NAR or HES affect relative mRNA concentration of genes involved in control of Tg metabolism. Serum Tg did not change, but gene expression of Thrsp, Lxr and Fat was lower after both, while Fas was decreased only after NAR. This is of great importance because treatment with NAR or HES could protect liver from fatty acids and Tg de novo biosynthesis and ectopic lipid accumulation, thus preventing liver steatosis and dyslipidemia accompanied with advanced age.
PB  - Belgrade: Serbian Physiological Society
C3  - Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia
T1  - Citrus flavanones alter the expression of genes involved in triglyceride metabolism in old age Wistar rats
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6024
ER  - 

@conference{
author = "Miler, Marko and Živanović, Jasmina and Ajdžanović, Vladimir and Jarić, Ivana and Šošić-Jurjević, Branka and Marković, Zorica and Potrebić, Milica and Milošević, Verica",
year = "2018",
abstract = "Advanced age is associated with development of dyslipidemia, together with ectopic triglycerides (Tg) accumulation in the liver. Consumption of citrus flavanones, naringenin (NAR) and hesperetin (HES) positively correlates with improvement of dyslipidemia in rats. However, information about this effects of citrus flavanones in old-aged rats still lack. Therefor we analyzed changes in gene expressions of liver X receptor (Lxr), thyroid hormone responsive spot14 protein (Thrsp), fatty acid synthase (Fas) and fatty acid translocase (Fat) in the liver by qPCR and serum Tg concentration biochemically. NAR or HES, suspended in vehicle (sunflower oil) were administrated orally (15 mg/kg) to male 24-month-old Wistar rats (n=5) during 4 weeks. Control groups received vehicle (CON) or stayed physiologically intact (ICON). In comparison to ICON animals, only Fat gene increased after sunflower oil administration. NAR and HES treatment decreased mRNA expression of Thrsp, Lxr and Fat genes in comparison to CON. Only NAR decreased Fas gene expression as well, while Tg concentration remained unchanged after both. All changes were statistically significant (p<0.05). In conclusion, nutritional doses of NAR or HES affect relative mRNA concentration of genes involved in control of Tg metabolism. Serum Tg did not change, but gene expression of Thrsp, Lxr and Fat was lower after both, while Fas was decreased only after NAR. This is of great importance because treatment with NAR or HES could protect liver from fatty acids and Tg de novo biosynthesis and ectopic lipid accumulation, thus preventing liver steatosis and dyslipidemia accompanied with advanced age.",
publisher = "Belgrade: Serbian Physiological Society",
journal = "Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia",
title = "Citrus flavanones alter the expression of genes involved in triglyceride metabolism in old age Wistar rats",
pages = "57",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6024"
}

Miler, M., Živanović, J., Ajdžanović, V., Jarić, I., Šošić-Jurjević, B., Marković, Z., Potrebić, M.,& Milošević, V.. (2018). Citrus flavanones alter the expression of genes involved in triglyceride metabolism in old age Wistar rats. in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia
Belgrade: Serbian Physiological Society., 57.
https://hdl.handle.net/21.15107/rcub_ibiss_6024

Miler M, Živanović J, Ajdžanović V, Jarić I, Šošić-Jurjević B, Marković Z, Potrebić M, Milošević V. Citrus flavanones alter the expression of genes involved in triglyceride metabolism in old age Wistar rats. in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia. 2018;:57.
https://hdl.handle.net/21.15107/rcub_ibiss_6024 .

Miler, Marko, Živanović, Jasmina, Ajdžanović, Vladimir, Jarić, Ivana, Šošić-Jurjević, Branka, Marković, Zorica, Potrebić, Milica, Milošević, Verica, "Citrus flavanones alter the expression of genes involved in triglyceride metabolism in old age Wistar rats" in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia (2018):57,
https://hdl.handle.net/21.15107/rcub_ibiss_6024 .

TY  - CONF
AU  - Ajdžanović, Vladimir
AU  - Jarić, Ivana
AU  - Popovska-Perčinić, Florina
AU  - Stojanović, Slobodan
AU  - Potrebić, Milica
AU  - Sretenović, Jasmina
AU  - Jakovljević, Vladimir
AU  - Milošević, Verica
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5992
AB  - Advanced age is frequently entwined with the adrenal cortex function impairment, glucocorticoid excess and following metabolic syndrome.
Also, age-dependent
disturbancies in aldosterone secretion and Na*/K homeostasis at least partly underlie the hypertension issues. Classical therapeutic approach in the treatment of some menopausal symptoms implies administration of estradiol or calcium, or their combination. Herein, we aimed to investigate the effects of chronic treatment with estradiol and calcium on some adrenal gland parameters in the ageing female rat model. Middle-aged (14-month-old) female Wistar rats were treated with estradiol dipropionate (EDP; 0.625 mg/kg b.m.) and calcium glucoheptonate (Ca; 11.4 mg/kg b.m.) daily for two weeks, while control ruts received vehicle alone by the same schedule. Combined treatment caused the decrease (p<0.05) of body mass by 21.5%, while the absolute and relative adrenal gland weights were increased (p<0.05) by16.2% and 43.8% respectively, compared to the adequate control values. The absolute adrenal gland volume, after treatment with EDP and Ca in the combination, was also increased (12.1%; p<0.05), in comparison with control group.
In parallel with that, circulating aldosterone was elevated (p<0.05) by 50.0%, compared to the control levels. However, combined treatment with EDP and Ca insignificantly (P>0.05) affected Na* and K' blood concentrations. It can be concluded that simultaneous estradiol ants calcium administration to middle-aged female rats enlarges the adrenal glands and have stimulatory influence on the mineralocorticoid output, which may represent unwanted effect from the perspective of blood pressure regulation.
PB  - Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine
C3  - Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia
T1  - Combined treatment with estradiol and calcium enlarges the adrenals and elevates circulating aldosterone in middle-aged female rats
SP  - 149
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5992
ER  - 

@conference{
author = "Ajdžanović, Vladimir and Jarić, Ivana and Popovska-Perčinić, Florina and Stojanović, Slobodan and Potrebić, Milica and Sretenović, Jasmina and Jakovljević, Vladimir and Milošević, Verica",
year = "2018",
abstract = "Advanced age is frequently entwined with the adrenal cortex function impairment, glucocorticoid excess and following metabolic syndrome.
Also, age-dependent
disturbancies in aldosterone secretion and Na*/K homeostasis at least partly underlie the hypertension issues. Classical therapeutic approach in the treatment of some menopausal symptoms implies administration of estradiol or calcium, or their combination. Herein, we aimed to investigate the effects of chronic treatment with estradiol and calcium on some adrenal gland parameters in the ageing female rat model. Middle-aged (14-month-old) female Wistar rats were treated with estradiol dipropionate (EDP; 0.625 mg/kg b.m.) and calcium glucoheptonate (Ca; 11.4 mg/kg b.m.) daily for two weeks, while control ruts received vehicle alone by the same schedule. Combined treatment caused the decrease (p<0.05) of body mass by 21.5%, while the absolute and relative adrenal gland weights were increased (p<0.05) by16.2% and 43.8% respectively, compared to the adequate control values. The absolute adrenal gland volume, after treatment with EDP and Ca in the combination, was also increased (12.1%; p<0.05), in comparison with control group.
In parallel with that, circulating aldosterone was elevated (p<0.05) by 50.0%, compared to the control levels. However, combined treatment with EDP and Ca insignificantly (P>0.05) affected Na* and K' blood concentrations. It can be concluded that simultaneous estradiol ants calcium administration to middle-aged female rats enlarges the adrenal glands and have stimulatory influence on the mineralocorticoid output, which may represent unwanted effect from the perspective of blood pressure regulation.",
publisher = "Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine",
journal = "Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia",
title = "Combined treatment with estradiol and calcium enlarges the adrenals and elevates circulating aldosterone in middle-aged female rats",
pages = "149",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5992"
}

Ajdžanović, V., Jarić, I., Popovska-Perčinić, F., Stojanović, S., Potrebić, M., Sretenović, J., Jakovljević, V.,& Milošević, V.. (2018). Combined treatment with estradiol and calcium enlarges the adrenals and elevates circulating aldosterone in middle-aged female rats. in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia
Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine., 149.
https://hdl.handle.net/21.15107/rcub_ibiss_5992

Ajdžanović V, Jarić I, Popovska-Perčinić F, Stojanović S, Potrebić M, Sretenović J, Jakovljević V, Milošević V. Combined treatment with estradiol and calcium enlarges the adrenals and elevates circulating aldosterone in middle-aged female rats. in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia. 2018;:149.
https://hdl.handle.net/21.15107/rcub_ibiss_5992 .

Ajdžanović, Vladimir, Jarić, Ivana, Popovska-Perčinić, Florina, Stojanović, Slobodan, Potrebić, Milica, Sretenović, Jasmina, Jakovljević, Vladimir, Milošević, Verica, "Combined treatment with estradiol and calcium enlarges the adrenals and elevates circulating aldosterone in middle-aged female rats" in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia (2018):149,
https://hdl.handle.net/21.15107/rcub_ibiss_5992 .

TY  - CONF
AU  - Popovska-Perčinić, Florina
AU  - Milošević, Verica
AU  - Pendovski, Lazo
AU  - Potrebić, Milica
AU  - Ušćebrka, Gordana
AU  - Sretenović, Jasmina
AU  - Jarić, Ivana
AU  - Ajdžanović, Vladimir
PY  - 2018
UR  - http://www.enea2018.com/program.html
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5925
PB  - European NeuroEndocrine Association
C3  - 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland
T1  - The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study
SP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5925
ER  - 

@conference{
author = "Popovska-Perčinić, Florina and Milošević, Verica and Pendovski, Lazo and Potrebić, Milica and Ušćebrka, Gordana and Sretenović, Jasmina and Jarić, Ivana and Ajdžanović, Vladimir",
year = "2018",
publisher = "European NeuroEndocrine Association",
journal = "18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland",
title = "The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study",
pages = "8",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5925"
}

Popovska-Perčinić, F., Milošević, V., Pendovski, L., Potrebić, M., Ušćebrka, G., Sretenović, J., Jarić, I.,& Ajdžanović, V.. (2018). The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study. in 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland
European NeuroEndocrine Association., 8.
https://hdl.handle.net/21.15107/rcub_ibiss_5925

Popovska-Perčinić F, Milošević V, Pendovski L, Potrebić M, Ušćebrka G, Sretenović J, Jarić I, Ajdžanović V. The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study. in 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland. 2018;:8.
https://hdl.handle.net/21.15107/rcub_ibiss_5925 .

Popovska-Perčinić, Florina, Milošević, Verica, Pendovski, Lazo, Potrebić, Milica, Ušćebrka, Gordana, Sretenović, Jasmina, Jarić, Ivana, Ajdžanović, Vladimir, "The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study" in 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland (2018):8,
https://hdl.handle.net/21.15107/rcub_ibiss_5925 .