TY  - CONF
AU  - Jelača, Sanja
AU  - Drača, Dijana
AU  - Dajić-Stevanović, Zora
AU  - Jovanović, Ivan
AU  - Pavlović, Sladjana
AU  - Gajović, Nevena
AU  - Mijatović, Sanja
AU  - Arsenijević, Nebojša
AU  - Maksimović-Ivanić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5297
AB  - Alchemilla vulgaris is well known for its diverse biological properties such as antiinflammatory,
antioxidant, wound healing, neuroprotective as well as for the treatment of female reproductive
system disorders. Our previous results showed that Alchemilla vulgaris agg. ethanol extract suppresses
the growth of melanoma cells in vitro and in vivo. The aim of this research was to evaluate the effect
of this extract on immune response in spleen and tumor microenvironment in syngeneic model of solid
melanoma. The obtained results strongly suggest that treatment with A. vulgaris extract significantly
modulates the systemic, as well as local intratumor immune response. A similar response was observed
in the spleen and tumor microenvironment. Applied treatment significantly increases the accumulation
of cytotoxic lymphocytes, while reducing the percentage of CD8+ cells that express inhibitory molecules
on their surface. On the other hand, treatment increased expression of cytotoxic activity markers of
CD8+ T cells derived from spleen and primary tumor. In line with this, A. vulgaris extract facilitates
maturation of dendritic cells making them efficient initiators as well as regulators of acquired immune
response to growing tumors. All mentioned above suggest that Alchemilla vulgaris agg. ethanol extract
diminishes immunosuppressive branch of immune response and stimulates the antitumor activities of
immune system through accumulation of cytotoxic lymphocytes and DCs maturation.
PB  - EFIS Young Immunologist Network (yEFIS Network)
C3  - Abstract Book: 1st Symposium: Shaping the Future of Immunology in Europe; 2022 Nov 10-11; Berlin, Germany
T1  - Alchemilla vulgaris agg ethanol extract enhances the antitumor immune response in syngeneic mouse melanoma model
DO  - 10.1002/eji.202270200
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5297
ER  - 

@conference{
author = "Jelača, Sanja and Drača, Dijana and Dajić-Stevanović, Zora and Jovanović, Ivan and Pavlović, Sladjana and Gajović, Nevena and Mijatović, Sanja and Arsenijević, Nebojša and Maksimović-Ivanić, Danijela",
year = "2022",
abstract = "Alchemilla vulgaris is well known for its diverse biological properties such as antiinflammatory,
antioxidant, wound healing, neuroprotective as well as for the treatment of female reproductive
system disorders. Our previous results showed that Alchemilla vulgaris agg. ethanol extract suppresses
the growth of melanoma cells in vitro and in vivo. The aim of this research was to evaluate the effect
of this extract on immune response in spleen and tumor microenvironment in syngeneic model of solid
melanoma. The obtained results strongly suggest that treatment with A. vulgaris extract significantly
modulates the systemic, as well as local intratumor immune response. A similar response was observed
in the spleen and tumor microenvironment. Applied treatment significantly increases the accumulation
of cytotoxic lymphocytes, while reducing the percentage of CD8+ cells that express inhibitory molecules
on their surface. On the other hand, treatment increased expression of cytotoxic activity markers of
CD8+ T cells derived from spleen and primary tumor. In line with this, A. vulgaris extract facilitates
maturation of dendritic cells making them efficient initiators as well as regulators of acquired immune
response to growing tumors. All mentioned above suggest that Alchemilla vulgaris agg. ethanol extract
diminishes immunosuppressive branch of immune response and stimulates the antitumor activities of
immune system through accumulation of cytotoxic lymphocytes and DCs maturation.",
publisher = "EFIS Young Immunologist Network (yEFIS Network)",
journal = "Abstract Book: 1st Symposium: Shaping the Future of Immunology in Europe; 2022 Nov 10-11; Berlin, Germany",
title = "Alchemilla vulgaris agg ethanol extract enhances the antitumor immune response in syngeneic mouse melanoma model",
doi = "10.1002/eji.202270200",
pages = "69",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5297"
}

Jelača, S., Drača, D., Dajić-Stevanović, Z., Jovanović, I., Pavlović, S., Gajović, N., Mijatović, S., Arsenijević, N.,& Maksimović-Ivanić, D.. (2022). Alchemilla vulgaris agg ethanol extract enhances the antitumor immune response in syngeneic mouse melanoma model. in Abstract Book: 1st Symposium: Shaping the Future of Immunology in Europe; 2022 Nov 10-11; Berlin, Germany
EFIS Young Immunologist Network (yEFIS Network)., 69.
https://doi.org/10.1002/eji.202270200
https://hdl.handle.net/21.15107/rcub_ibiss_5297

Jelača S, Drača D, Dajić-Stevanović Z, Jovanović I, Pavlović S, Gajović N, Mijatović S, Arsenijević N, Maksimović-Ivanić D. Alchemilla vulgaris agg ethanol extract enhances the antitumor immune response in syngeneic mouse melanoma model. in Abstract Book: 1st Symposium: Shaping the Future of Immunology in Europe; 2022 Nov 10-11; Berlin, Germany. 2022;:69.
doi:10.1002/eji.202270200
https://hdl.handle.net/21.15107/rcub_ibiss_5297 .

Jelača, Sanja, Drača, Dijana, Dajić-Stevanović, Zora, Jovanović, Ivan, Pavlović, Sladjana, Gajović, Nevena, Mijatović, Sanja, Arsenijević, Nebojša, Maksimović-Ivanić, Danijela, "Alchemilla vulgaris agg ethanol extract enhances the antitumor immune response in syngeneic mouse melanoma model" in Abstract Book: 1st Symposium: Shaping the Future of Immunology in Europe; 2022 Nov 10-11; Berlin, Germany (2022):69,
https://doi.org/10.1002/eji.202270200 .,
https://hdl.handle.net/21.15107/rcub_ibiss_5297 .

TY  - CONF
AU  - Jelača, Sanja
AU  - Drača, Dijana
AU  - Dajić Stevanović, Zora
AU  - Jovanović, Ivan
AU  - Pavlović, Slađana
AU  - Gajović, Nevena
AU  - Mijatović, Sanja
AU  - Arsenijević, Nebojša
AU  - Maksimović-Ivanić, Danijela
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4432
UR  - https://onlinelibrary.wiley.com/toc/15214141/2021/51/S1
AB  - Several ethnobotanical reports on Alchemilla vulgaris L. pointed out diverse biological properties against problems such as dysmenorrhea, pruritus vulvae, menopausal complaints 
as  well  as  related  diseases  in  women.  Also  previous  studies  have  shown  that  Alchemilla  vulgaris  L.  extracts  are  exhibiting  antiinflammatory,  antioxidant,  wound  healing  and 
neuroprotective activity. The aim of this study was to evaluate the direct effect of Alchemilla vulgaris L. ethanol extract against melanoma cells in vitro and in vivo, as well as its effect 
on tumor microenvironment ex vivo. This study was performed on two different mouse melanoma cell lines, B16 and B16F10, and on syngeneic mouse melanoma model in vivo. 
Obtained results revealed dose‐dependent decrease of cell viability after 72 h‐ treatment with Alchemilla vulgaris L. extract. The observed effect was followed by loss of dividing 
potential in both tested cell lines. In parallel with this, certain percentage of B16F10 cells was subjected to programmed cell death in a caspase independent manner while in B16 cells 
estimation of the presence of autophagosomes by flow cytometry has shown that autophagy is occurring after the treatment and it is shown to be mechanism of death. Concerning in 
vivo studies Alchemilla vulgaris L. extract significantly reduced tumor growth in B16 melanoma model partly through stimulation of antitumor immune responce. It altered dendritic 
cells phenotype which activated cytotoxic and CD4+ T lymphocytes to successfully destroy tumor cells. In summary, these data indicate that Alchemilla vulgaris L. is valuable of further 
investigation in the field of experimental oncology.
PB  - Wiley‐VCH GmbH
C3  - 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
T1  - Multiple effects of Alchemilla vulgaris L. extract on melanoma cells and tumor microenvironment
IS  - Suppl 1
VL  - 51
DO  - 10.1002/eji.202170200
SP  - 352
ER  - 

@conference{
author = "Jelača, Sanja and Drača, Dijana and Dajić Stevanović, Zora and Jovanović, Ivan and Pavlović, Slađana and Gajović, Nevena and Mijatović, Sanja and Arsenijević, Nebojša and Maksimović-Ivanić, Danijela",
year = "2021",
abstract = "Several ethnobotanical reports on Alchemilla vulgaris L. pointed out diverse biological properties against problems such as dysmenorrhea, pruritus vulvae, menopausal complaints 
as  well  as  related  diseases  in  women.  Also  previous  studies  have  shown  that  Alchemilla  vulgaris  L.  extracts  are  exhibiting  antiinflammatory,  antioxidant,  wound  healing  and 
neuroprotective activity. The aim of this study was to evaluate the direct effect of Alchemilla vulgaris L. ethanol extract against melanoma cells in vitro and in vivo, as well as its effect 
on tumor microenvironment ex vivo. This study was performed on two different mouse melanoma cell lines, B16 and B16F10, and on syngeneic mouse melanoma model in vivo. 
Obtained results revealed dose‐dependent decrease of cell viability after 72 h‐ treatment with Alchemilla vulgaris L. extract. The observed effect was followed by loss of dividing 
potential in both tested cell lines. In parallel with this, certain percentage of B16F10 cells was subjected to programmed cell death in a caspase independent manner while in B16 cells 
estimation of the presence of autophagosomes by flow cytometry has shown that autophagy is occurring after the treatment and it is shown to be mechanism of death. Concerning in 
vivo studies Alchemilla vulgaris L. extract significantly reduced tumor growth in B16 melanoma model partly through stimulation of antitumor immune responce. It altered dendritic 
cells phenotype which activated cytotoxic and CD4+ T lymphocytes to successfully destroy tumor cells. In summary, these data indicate that Alchemilla vulgaris L. is valuable of further 
investigation in the field of experimental oncology.",
publisher = "Wiley‐VCH GmbH",
journal = "6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting",
title = "Multiple effects of Alchemilla vulgaris L. extract on melanoma cells and tumor microenvironment",
number = "Suppl 1",
volume = "51",
doi = "10.1002/eji.202170200",
pages = "352"
}

Jelača, S., Drača, D., Dajić Stevanović, Z., Jovanović, I., Pavlović, S., Gajović, N., Mijatović, S., Arsenijević, N.,& Maksimović-Ivanić, D.. (2021). Multiple effects of Alchemilla vulgaris L. extract on melanoma cells and tumor microenvironment. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
Wiley‐VCH GmbH., 51(Suppl 1), 352.
https://doi.org/10.1002/eji.202170200

Jelača S, Drača D, Dajić Stevanović Z, Jovanović I, Pavlović S, Gajović N, Mijatović S, Arsenijević N, Maksimović-Ivanić D. Multiple effects of Alchemilla vulgaris L. extract on melanoma cells and tumor microenvironment. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting. 2021;51(Suppl 1):352.
doi:10.1002/eji.202170200 .

Jelača, Sanja, Drača, Dijana, Dajić Stevanović, Zora, Jovanović, Ivan, Pavlović, Slađana, Gajović, Nevena, Mijatović, Sanja, Arsenijević, Nebojša, Maksimović-Ivanić, Danijela, "Multiple effects of Alchemilla vulgaris L. extract on melanoma cells and tumor microenvironment" in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting, 51, no. Suppl 1 (2021):352,
https://doi.org/10.1002/eji.202170200 . .