TY  - JOUR
AU  - Aleksić, Marija
AU  - Golić, Igor
AU  - Janković, Aleksandra
AU  - Čvoro, Aleksandra
AU  - Korać, Aleksandra
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6529
AB  - We previously demonstrated that hypothyroidism increases peroxisomal biogenesis in rat brown adipose tissue (BAT). We also showed heterogeneity in peroxisomal origin and their unique structural association with mitochondria and/or lipid bodies to carry out β-oxidation, contributing thus to BAT thermogenesis. Distinctive heterogeneity creates structural compartmentalization within peroxisomal population, raising the question of whether it is followed by their functional compartmentalization regarding localization/colocalization of two main acyl-CoA oxidase (ACOX) isoforms, ACOX1 and ACOX3. ACOX is the first and rate-limiting enzyme of peroxisomal β-oxidation, and, to date, their protein expression patterns in BAT have not been fully defined. Therefore, we used methimazole-induced hypothyroidism to study ACOX1 and ACOX3 protein expression and their tissue immunolocalization. Additionally, we analysed their specific peroxisomal localization and colocalization in parallel with peroxisomal structural compartmentalization in brown adipocytes. Hypothyroidism caused a linear increase in ACOX1 expression, while a temporary decrease in ACOX3 levels is only recovered to the control level at day 21. Peroxisomal ACOX1 and ACOX3 localization and colocalization patterns entirely mirrored heterogeneous peroxisomal biogenesis pathways and structural compartmentalization, e.g. associations with mitochondria and/or lipid bodies. Hence, different ACOX isoforms localization/colocalization creates distinct functional heterogeneity of peroxisomes and drives their functional compartmentalization in rat brown adipocytes.
PB  - London: Royal society publishing
T2  - Royal Society Open Science
T1  - ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats
VL  - 10
DO  - 10.1098/rsos.230109
SP  - 230109
ER  - 

@article{
author = "Aleksić, Marija and Golić, Igor and Janković, Aleksandra and Čvoro, Aleksandra and Korać, Aleksandra",
year = "2023",
abstract = "We previously demonstrated that hypothyroidism increases peroxisomal biogenesis in rat brown adipose tissue (BAT). We also showed heterogeneity in peroxisomal origin and their unique structural association with mitochondria and/or lipid bodies to carry out β-oxidation, contributing thus to BAT thermogenesis. Distinctive heterogeneity creates structural compartmentalization within peroxisomal population, raising the question of whether it is followed by their functional compartmentalization regarding localization/colocalization of two main acyl-CoA oxidase (ACOX) isoforms, ACOX1 and ACOX3. ACOX is the first and rate-limiting enzyme of peroxisomal β-oxidation, and, to date, their protein expression patterns in BAT have not been fully defined. Therefore, we used methimazole-induced hypothyroidism to study ACOX1 and ACOX3 protein expression and their tissue immunolocalization. Additionally, we analysed their specific peroxisomal localization and colocalization in parallel with peroxisomal structural compartmentalization in brown adipocytes. Hypothyroidism caused a linear increase in ACOX1 expression, while a temporary decrease in ACOX3 levels is only recovered to the control level at day 21. Peroxisomal ACOX1 and ACOX3 localization and colocalization patterns entirely mirrored heterogeneous peroxisomal biogenesis pathways and structural compartmentalization, e.g. associations with mitochondria and/or lipid bodies. Hence, different ACOX isoforms localization/colocalization creates distinct functional heterogeneity of peroxisomes and drives their functional compartmentalization in rat brown adipocytes.",
publisher = "London: Royal society publishing",
journal = "Royal Society Open Science",
title = "ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats",
volume = "10",
doi = "10.1098/rsos.230109",
pages = "230109"
}

Aleksić, M., Golić, I., Janković, A., Čvoro, A.,& Korać, A.. (2023). ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats. in Royal Society Open Science
London: Royal society publishing., 10, 230109.
https://doi.org/10.1098/rsos.230109

Aleksić M, Golić I, Janković A, Čvoro A, Korać A. ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats. in Royal Society Open Science. 2023;10:230109.
doi:10.1098/rsos.230109 .

Aleksić, Marija, Golić, Igor, Janković, Aleksandra, Čvoro, Aleksandra, Korać, Aleksandra, "ACOX-driven peroxisomal heterogeneity and functional compartmentalization in brown adipocytes of hypothyroid rats" in Royal Society Open Science, 10 (2023):230109,
https://doi.org/10.1098/rsos.230109 . .

TY  - JOUR
AU  - Protić, Isidora
AU  - Golić, Igor
AU  - Vidaković, Snežana
AU  - Korać, Bato
AU  - Korać, Aleksandra
PY  - 2022
UR  - https://www.mdpi.com/1467-3045/44/8/237
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9406340
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5110
AB  - Zinc (in the form of Zn2+) is necessary for male fertility. Both Zn2+ quantity and its localisation have been detected in seminal plasma and ejaculated spermatozoa, suggesting its active uptake via zinc import transporters (ZIPs). Immunofluorescence was used to characterise the expression and localisation of three distinct types of ZIP transporters in ejaculated spermatozoa of normo- and asthenozoospermic sperm samples. ZIP6, ZIP10 and ZIP14 showed heterogeneous sperm cell expression and different compartmental distribution. In both types of sperm samples, ZIP6 and ZIP14 were predominantly localised in the sperm head, while ZIP10 was found along the sperm tail. Compartmental localisation of ZIPs in asthenozoospermia was not changed. However, regarding sub-compartmental localisation in sperm head regions, for ZIP6 asthenozoospermia only decreased its acorn/crescent-like pattern. In contrast, ZIP14 immunostaining was altered in favour of crescent-like, as opposed to acorn-like and acorn/crescent-like patterns. The specific ZIPs localisation may reflect their different roles in sperm cell integrity and motility and may change over time. This is the first report of their specific compartmental and sub-compartmental localisation in ejaculated human sperm cells. Further research will lead to a greater understanding of the roles of ZIPs in sperm cell biology, which could positively influence procedures for human infertility therapy.
PB  - Basel: MDPI
T2  - Current Issues in Molecular Biology
T1  - Heterogeneous Immunolocalisation of Zinc Transporters ZIP6, ZIP10 and ZIP14 in Human Normo- and Asthenozoospermic Spermatozoa.
IS  - 8
VL  - 44
DO  - 10.3390/cimb44080237
SP  - 3444
EP  - 3454
ER  - 

@article{
author = "Protić, Isidora and Golić, Igor and Vidaković, Snežana and Korać, Bato and Korać, Aleksandra",
year = "2022",
abstract = "Zinc (in the form of Zn2+) is necessary for male fertility. Both Zn2+ quantity and its localisation have been detected in seminal plasma and ejaculated spermatozoa, suggesting its active uptake via zinc import transporters (ZIPs). Immunofluorescence was used to characterise the expression and localisation of three distinct types of ZIP transporters in ejaculated spermatozoa of normo- and asthenozoospermic sperm samples. ZIP6, ZIP10 and ZIP14 showed heterogeneous sperm cell expression and different compartmental distribution. In both types of sperm samples, ZIP6 and ZIP14 were predominantly localised in the sperm head, while ZIP10 was found along the sperm tail. Compartmental localisation of ZIPs in asthenozoospermia was not changed. However, regarding sub-compartmental localisation in sperm head regions, for ZIP6 asthenozoospermia only decreased its acorn/crescent-like pattern. In contrast, ZIP14 immunostaining was altered in favour of crescent-like, as opposed to acorn-like and acorn/crescent-like patterns. The specific ZIPs localisation may reflect their different roles in sperm cell integrity and motility and may change over time. This is the first report of their specific compartmental and sub-compartmental localisation in ejaculated human sperm cells. Further research will lead to a greater understanding of the roles of ZIPs in sperm cell biology, which could positively influence procedures for human infertility therapy.",
publisher = "Basel: MDPI",
journal = "Current Issues in Molecular Biology",
title = "Heterogeneous Immunolocalisation of Zinc Transporters ZIP6, ZIP10 and ZIP14 in Human Normo- and Asthenozoospermic Spermatozoa.",
number = "8",
volume = "44",
doi = "10.3390/cimb44080237",
pages = "3444-3454"
}

Protić, I., Golić, I., Vidaković, S., Korać, B.,& Korać, A.. (2022). Heterogeneous Immunolocalisation of Zinc Transporters ZIP6, ZIP10 and ZIP14 in Human Normo- and Asthenozoospermic Spermatozoa.. in Current Issues in Molecular Biology
Basel: MDPI., 44(8), 3444-3454.
https://doi.org/10.3390/cimb44080237

Protić I, Golić I, Vidaković S, Korać B, Korać A. Heterogeneous Immunolocalisation of Zinc Transporters ZIP6, ZIP10 and ZIP14 in Human Normo- and Asthenozoospermic Spermatozoa.. in Current Issues in Molecular Biology. 2022;44(8):3444-3454.
doi:10.3390/cimb44080237 .

Protić, Isidora, Golić, Igor, Vidaković, Snežana, Korać, Bato, Korać, Aleksandra, "Heterogeneous Immunolocalisation of Zinc Transporters ZIP6, ZIP10 and ZIP14 in Human Normo- and Asthenozoospermic Spermatozoa." in Current Issues in Molecular Biology, 44, no. 8 (2022):3444-3454,
https://doi.org/10.3390/cimb44080237 . .

TY  - JOUR
AU  - Protić, Isidora
AU  - Golić, Igor
AU  - Aleksić, Marija
AU  - Vidaković, Snežana
AU  - Korać, Bato
AU  - Korać, Aleksandra
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0306987722000408
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4862
AB  - The global rise in male infertility necessitates a constant search for underlying causes, diagnostics and corrective treatments. Sperm cells are irreplaceable cells for the process of reproduction. Intrauterine insemination (IUI) is a simple and non-invasive technique, most commonly used to overcome both unexplained and mild male-factor infertility. Even though spermatozoa may be classified as “normal” in terms of morphology, motility and concentration, they could be defective at the subcellular level. Protein lysine acetylation within human sperm, a major post-translational modification of tubulin, is suspected to be a cause of sperm abnormality but is not fully understood. An examination of α-tubulin and acetylated α-tubulin immunopresence, spatial distribution and co-localisation in normozoospermic and asthenozoospermic semen samples was conducted to determine if acetylated α-tubulin could be a biomarker of sperm heterogeneity to better predict sperm fertility potential for IUI outcome. Acetylated α-tubulin was present in both sperm subcompartments, tail and nucleus of normozoospermic samples. We found more immunopositivity for acetylated α-tubulin in the sperm tail and less in nucleus of asthenozoospermic compared to normozoospermic samples. Hence, the acetylated α-tubulin in sperm may contribute to sperm nuclei heterogeneity and serve as a novel molecular biomarker.
PB  - Edinburgh: Churchill Livingstone
T2  - Medical Hypotheses
T1  - Presence of acetylated α-tubulin in human sperm nuclei: A contributor to sperm heterogeneity
VL  - 161
DO  - 10.1016/j.mehy.2022.110800
SP  - 110800
ER  - 

@article{
author = "Protić, Isidora and Golić, Igor and Aleksić, Marija and Vidaković, Snežana and Korać, Bato and Korać, Aleksandra",
year = "2022",
abstract = "The global rise in male infertility necessitates a constant search for underlying causes, diagnostics and corrective treatments. Sperm cells are irreplaceable cells for the process of reproduction. Intrauterine insemination (IUI) is a simple and non-invasive technique, most commonly used to overcome both unexplained and mild male-factor infertility. Even though spermatozoa may be classified as “normal” in terms of morphology, motility and concentration, they could be defective at the subcellular level. Protein lysine acetylation within human sperm, a major post-translational modification of tubulin, is suspected to be a cause of sperm abnormality but is not fully understood. An examination of α-tubulin and acetylated α-tubulin immunopresence, spatial distribution and co-localisation in normozoospermic and asthenozoospermic semen samples was conducted to determine if acetylated α-tubulin could be a biomarker of sperm heterogeneity to better predict sperm fertility potential for IUI outcome. Acetylated α-tubulin was present in both sperm subcompartments, tail and nucleus of normozoospermic samples. We found more immunopositivity for acetylated α-tubulin in the sperm tail and less in nucleus of asthenozoospermic compared to normozoospermic samples. Hence, the acetylated α-tubulin in sperm may contribute to sperm nuclei heterogeneity and serve as a novel molecular biomarker.",
publisher = "Edinburgh: Churchill Livingstone",
journal = "Medical Hypotheses",
title = "Presence of acetylated α-tubulin in human sperm nuclei: A contributor to sperm heterogeneity",
volume = "161",
doi = "10.1016/j.mehy.2022.110800",
pages = "110800"
}

Protić, I., Golić, I., Aleksić, M., Vidaković, S., Korać, B.,& Korać, A.. (2022). Presence of acetylated α-tubulin in human sperm nuclei: A contributor to sperm heterogeneity. in Medical Hypotheses
Edinburgh: Churchill Livingstone., 161, 110800.
https://doi.org/10.1016/j.mehy.2022.110800

Protić I, Golić I, Aleksić M, Vidaković S, Korać B, Korać A. Presence of acetylated α-tubulin in human sperm nuclei: A contributor to sperm heterogeneity. in Medical Hypotheses. 2022;161:110800.
doi:10.1016/j.mehy.2022.110800 .

Protić, Isidora, Golić, Igor, Aleksić, Marija, Vidaković, Snežana, Korać, Bato, Korać, Aleksandra, "Presence of acetylated α-tubulin in human sperm nuclei: A contributor to sperm heterogeneity" in Medical Hypotheses, 161 (2022):110800,
https://doi.org/10.1016/j.mehy.2022.110800 . .

TY  - CONF
AU  - Golić, Igor
AU  - Krajnović, Tamara
AU  - Jelača, Sanja
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Koruga, Đuro
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5296
AB  - Fullerene C60 is the third allotropic modification of carbon in nature. Exceptional physical and
structural features of C60 molecule make it attractive for biomedical research in a wide spectrum of
applications from imaging, drug delivery, to therapy of different disorders. However, its low
solubility in water or polar solvents, and genotoxic potential represent serious barriers for its usage.
To overcome mentioned limits the second derivative of fullerene C60 (Hyper harmonized
hydroxylated fullerene water complex - 3HFWC) is created through functionalization of the first
fullerene C60 derivative (fullerol - C60(OH)x) by the addition of paired OH groups in water layers
surrounding the solid phase of substance. The solid phase consists of the C60 molecule, covalent OH
groups and 3-6 water layers with strong hydrogen bonds. The liquid phase consists of additional
water layers bonded by moderate to weak hydrogen bonds. In addition, the third derivative of
fullerene is made when 3HFWC was integrated with nano-gold particles (nAu@3HFWC). The aim
of this study was to evaluate fullerene C60 derivatives uptake and their intracellular distribution in
mouse 3T3 fibroblasts. Cells were exposed to 30 μg/ml C60(OH)x, or 30 μg/ml 3HFWC, or 5μg/ml
nAu@3HFWC during 30 min, 1h or 2h and analysis was done by Philips FEI CM12 transmission
electron microscope. Ultrastructural analysis showed mainly cytoplasmic localization of C60(OH)x
and its derivatives, and also a sporadic presence in mitochondria and nuclei. The concentration of
fullerene derivatives in these cells was time-dependent, i.e., more concentration was observed in
cells with longer exposition to these fullerenes. Furthermore, C60(OH)x is also localized in lysosomes
in higher concentrations. These results suggest that novel C60 fullerene derivatives show better
biocompatibility in mouse 3T3 fibroblasts compared to C60(OH)x, which mainly ends in lysosomes,
the waste disposal system of the cell.
PB  - Belgrade: Serbian Academy of Sciences and Arts
C3  - Program and Book of Abstracts: Second International Conference ELMINA; 2022 Aug 22-26. Belgrade, Serbia
T1  - Investigation of the Action of the Fullerene (C60) Derivatives on Mouse 3T3 Fibroblast Cell Lines
SP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5296
ER  - 

@conference{
author = "Golić, Igor and Krajnović, Tamara and Jelača, Sanja and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Koruga, Đuro",
year = "2022",
abstract = "Fullerene C60 is the third allotropic modification of carbon in nature. Exceptional physical and
structural features of C60 molecule make it attractive for biomedical research in a wide spectrum of
applications from imaging, drug delivery, to therapy of different disorders. However, its low
solubility in water or polar solvents, and genotoxic potential represent serious barriers for its usage.
To overcome mentioned limits the second derivative of fullerene C60 (Hyper harmonized
hydroxylated fullerene water complex - 3HFWC) is created through functionalization of the first
fullerene C60 derivative (fullerol - C60(OH)x) by the addition of paired OH groups in water layers
surrounding the solid phase of substance. The solid phase consists of the C60 molecule, covalent OH
groups and 3-6 water layers with strong hydrogen bonds. The liquid phase consists of additional
water layers bonded by moderate to weak hydrogen bonds. In addition, the third derivative of
fullerene is made when 3HFWC was integrated with nano-gold particles (nAu@3HFWC). The aim
of this study was to evaluate fullerene C60 derivatives uptake and their intracellular distribution in
mouse 3T3 fibroblasts. Cells were exposed to 30 μg/ml C60(OH)x, or 30 μg/ml 3HFWC, or 5μg/ml
nAu@3HFWC during 30 min, 1h or 2h and analysis was done by Philips FEI CM12 transmission
electron microscope. Ultrastructural analysis showed mainly cytoplasmic localization of C60(OH)x
and its derivatives, and also a sporadic presence in mitochondria and nuclei. The concentration of
fullerene derivatives in these cells was time-dependent, i.e., more concentration was observed in
cells with longer exposition to these fullerenes. Furthermore, C60(OH)x is also localized in lysosomes
in higher concentrations. These results suggest that novel C60 fullerene derivatives show better
biocompatibility in mouse 3T3 fibroblasts compared to C60(OH)x, which mainly ends in lysosomes,
the waste disposal system of the cell.",
publisher = "Belgrade: Serbian Academy of Sciences and Arts",
journal = "Program and Book of Abstracts: Second International Conference ELMINA; 2022 Aug 22-26. Belgrade, Serbia",
title = "Investigation of the Action of the Fullerene (C60) Derivatives on Mouse 3T3 Fibroblast Cell Lines",
pages = "84",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5296"
}

Golić, I., Krajnović, T., Jelača, S., Mijatović, S., Maksimović-Ivanić, D.,& Koruga, Đ.. (2022). Investigation of the Action of the Fullerene (C60) Derivatives on Mouse 3T3 Fibroblast Cell Lines. in Program and Book of Abstracts: Second International Conference ELMINA; 2022 Aug 22-26. Belgrade, Serbia
Belgrade: Serbian Academy of Sciences and Arts., 84.
https://hdl.handle.net/21.15107/rcub_ibiss_5296

Golić I, Krajnović T, Jelača S, Mijatović S, Maksimović-Ivanić D, Koruga Đ. Investigation of the Action of the Fullerene (C60) Derivatives on Mouse 3T3 Fibroblast Cell Lines. in Program and Book of Abstracts: Second International Conference ELMINA; 2022 Aug 22-26. Belgrade, Serbia. 2022;:84.
https://hdl.handle.net/21.15107/rcub_ibiss_5296 .

Golić, Igor, Krajnović, Tamara, Jelača, Sanja, Mijatović, Sanja, Maksimović-Ivanić, Danijela, Koruga, Đuro, "Investigation of the Action of the Fullerene (C60) Derivatives on Mouse 3T3 Fibroblast Cell Lines" in Program and Book of Abstracts: Second International Conference ELMINA; 2022 Aug 22-26. Belgrade, Serbia (2022):84,
https://hdl.handle.net/21.15107/rcub_ibiss_5296 .

TY  - JOUR
AU  - Aleksić, Marija
AU  - Golić, Igor
AU  - Kalezić, Anđelika
AU  - Janković, Aleksandra
AU  - Korać, Bato
AU  - Korać, Aleksandra
PY  - 2021
UR  - https://www.mdpi.com/2073-4409/10/9/2248
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8472630
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4491
AB  - Despite peroxisomes being important partners of mitochondria by carrying out fatty acid oxidation in brown adipocytes, no clear evidence concerning peroxisome origin and way(s) of biogenesis exists. Herein we used methimazole-induced hypothyroidism for 7, 15, and 21 days to study peroxisomal remodeling and origin in rat brown adipocytes. We found that peroxisomes originated via both canonic, and de novo pathways. Each pathway operates in euthyroid control and over the course of hypothyroidism, in a time-dependent manner. Hypothyroidism increased the peroxisomal number by 1.8-, 3.6- and 5.8-fold on days 7, 15, and 21. Peroxisomal presence, their distribution, and their degree of maturation were heterogeneous in brown adipocytes in a Harlequin-like manner, reflecting differences in their origin. The canonic pathway, through numerous dumbbell-like and "pearls on strings" structures, supported by high levels of Pex11β and Drp1, prevailed on day 7. The de novo pathway of peroxisomal biogenesis started on day 15 and became dominant by day 21. The transition of peroxisomal biogenesis from canonic to the de novo pathway was driven by increased levels of Pex19, PMP70, Pex5S, and Pex26 and characterized by numerous tubular structures. Furthermore, specific peroxisomal origin from mitochondria, regardless of thyroid status, indicates their mutual regulation in rat brown adipocytes.
PB  - Basel: MDPI
T2  - Cells
T1  - Hypothyroidism Intensifies Both Canonic and the De Novo Pathway of Peroxisomal Biogenesis in Rat Brown Adipocytes in a Time-Dependent Manner.
IS  - 9
VL  - 10
DO  - 10.3390/cells10092248
SP  - 2248
ER  - 

@article{
author = "Aleksić, Marija and Golić, Igor and Kalezić, Anđelika and Janković, Aleksandra and Korać, Bato and Korać, Aleksandra",
year = "2021",
abstract = "Despite peroxisomes being important partners of mitochondria by carrying out fatty acid oxidation in brown adipocytes, no clear evidence concerning peroxisome origin and way(s) of biogenesis exists. Herein we used methimazole-induced hypothyroidism for 7, 15, and 21 days to study peroxisomal remodeling and origin in rat brown adipocytes. We found that peroxisomes originated via both canonic, and de novo pathways. Each pathway operates in euthyroid control and over the course of hypothyroidism, in a time-dependent manner. Hypothyroidism increased the peroxisomal number by 1.8-, 3.6- and 5.8-fold on days 7, 15, and 21. Peroxisomal presence, their distribution, and their degree of maturation were heterogeneous in brown adipocytes in a Harlequin-like manner, reflecting differences in their origin. The canonic pathway, through numerous dumbbell-like and "pearls on strings" structures, supported by high levels of Pex11β and Drp1, prevailed on day 7. The de novo pathway of peroxisomal biogenesis started on day 15 and became dominant by day 21. The transition of peroxisomal biogenesis from canonic to the de novo pathway was driven by increased levels of Pex19, PMP70, Pex5S, and Pex26 and characterized by numerous tubular structures. Furthermore, specific peroxisomal origin from mitochondria, regardless of thyroid status, indicates their mutual regulation in rat brown adipocytes.",
publisher = "Basel: MDPI",
journal = "Cells",
title = "Hypothyroidism Intensifies Both Canonic and the De Novo Pathway of Peroxisomal Biogenesis in Rat Brown Adipocytes in a Time-Dependent Manner.",
number = "9",
volume = "10",
doi = "10.3390/cells10092248",
pages = "2248"
}

Aleksić, M., Golić, I., Kalezić, A., Janković, A., Korać, B.,& Korać, A.. (2021). Hypothyroidism Intensifies Both Canonic and the De Novo Pathway of Peroxisomal Biogenesis in Rat Brown Adipocytes in a Time-Dependent Manner.. in Cells
Basel: MDPI., 10(9), 2248.
https://doi.org/10.3390/cells10092248

Aleksić M, Golić I, Kalezić A, Janković A, Korać B, Korać A. Hypothyroidism Intensifies Both Canonic and the De Novo Pathway of Peroxisomal Biogenesis in Rat Brown Adipocytes in a Time-Dependent Manner.. in Cells. 2021;10(9):2248.
doi:10.3390/cells10092248 .

Aleksić, Marija, Golić, Igor, Kalezić, Anđelika, Janković, Aleksandra, Korać, Bato, Korać, Aleksandra, "Hypothyroidism Intensifies Both Canonic and the De Novo Pathway of Peroxisomal Biogenesis in Rat Brown Adipocytes in a Time-Dependent Manner." in Cells, 10, no. 9 (2021):2248,
https://doi.org/10.3390/cells10092248 . .

TY  - CONF
AU  - Golić, Igor
AU  - Aleksić, Marija
AU  - Kalezić, Anđelika
AU  - Janković, Aleksandra
AU  - Korać, Bato
AU  - Korać, Aleksandra
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4803
AB  - The disequilibrium of reactive oxygen/nitrogen species (ROS/RNS) is one of the causes of male infertility. To examine the role of ROS/RNS in reproduction, we modulated the cell concentration of О2•– and NO using the superoxide dismutase (SOD) mimic, M40403, which selectively removes О2•– and consequently increases the NO bioavailability. The fine chromatin changes are difficult to observe via routine light/electron microscopy, therefore we used fractal analysis to analyze DNA compaction. We used normospermic semen samples from ten human subjects. After purification in Cook density gradient, the sperm-rich fraction was rinsed in modified Tyrod medium (TM). Purified samples were divided into three groups. One group was evaluated immediately after resuspension in TM and served as the control. For the other two groups, untreated and SOD mimic-treated group (50 μM), the sperm was resuspended in TM and evaluated after incubation at 37 ºC / 6% CO2 for 3 hours. Air-dried and methanol fixed sperm smears were stained with toluidine blue and analyzed on Leica DMLB microscope. Images from ten randomly selected fields were analyzed in ImageJ plugin FracLac to get fractal dimension defined as a measure of complexity. Average values of fractal dimensions (mean ± SEM) are: control group – 1.016 ± 0.553; untreated group – 0.955 ± 0.291; treated group – 1.146 ± 0.096. Also, average values of lacunarity are: control group – 0.734 ± 0.129; untreated group – 0.727 ± 0.129; treated group – 0.901 ± 0.297. Results show that SOD mimic remodels chromatin condensation by increasing the euchromatin area, potentially leading to a resume in transcriptional activity. This hypothesis would be consistent with our published findings of up-regulated mRNA expression of eNOS, MnSOD, and catalase in SOD mimic-treated spermatozoa ex vivo (Otasevic et al., 2013). Therefore, SOD mimic modulates the redox environment via direct chromatin remodeling in spermatozoa. Fractal analysis has proven to be a good method for the analysis of chromatin condensation in human sperm nuclei. Ref: Otasevic V, Korac A, Vucetic M, Macanovic B, Garalejic E, Ivanovic-Burmazovic I, Filipovic MR, Buzadzic B, Stancic A, Jankovic A, Velickovic K, Golic I, Markelic M, Korac B. Is manganese (II) pentaazamacrocyclic superoxide dismutase mimic beneficial for human sperm mitochondria function and motility? Antioxid Redox Signal. 2013 Jan 10;18(2):170-8. doi: 10.1089/ars.2012.4684. Epub 2012 Jun 12. PMID: 22563824; PMCID: PMC3513981
PB  - Elsevier Inc.
C3  - Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia
T1  - Fractal analysis of chromatin condensation in the human sperm nuclei
DO  - 10.1016/j.freeradbiomed.2021.08.178
SP  - S114
ER  - 

@conference{
author = "Golić, Igor and Aleksić, Marija and Kalezić, Anđelika and Janković, Aleksandra and Korać, Bato and Korać, Aleksandra",
year = "2021",
abstract = "The disequilibrium of reactive oxygen/nitrogen species (ROS/RNS) is one of the causes of male infertility. To examine the role of ROS/RNS in reproduction, we modulated the cell concentration of О2•– and NO using the superoxide dismutase (SOD) mimic, M40403, which selectively removes О2•– and consequently increases the NO bioavailability. The fine chromatin changes are difficult to observe via routine light/electron microscopy, therefore we used fractal analysis to analyze DNA compaction. We used normospermic semen samples from ten human subjects. After purification in Cook density gradient, the sperm-rich fraction was rinsed in modified Tyrod medium (TM). Purified samples were divided into three groups. One group was evaluated immediately after resuspension in TM and served as the control. For the other two groups, untreated and SOD mimic-treated group (50 μM), the sperm was resuspended in TM and evaluated after incubation at 37 ºC / 6% CO2 for 3 hours. Air-dried and methanol fixed sperm smears were stained with toluidine blue and analyzed on Leica DMLB microscope. Images from ten randomly selected fields were analyzed in ImageJ plugin FracLac to get fractal dimension defined as a measure of complexity. Average values of fractal dimensions (mean ± SEM) are: control group – 1.016 ± 0.553; untreated group – 0.955 ± 0.291; treated group – 1.146 ± 0.096. Also, average values of lacunarity are: control group – 0.734 ± 0.129; untreated group – 0.727 ± 0.129; treated group – 0.901 ± 0.297. Results show that SOD mimic remodels chromatin condensation by increasing the euchromatin area, potentially leading to a resume in transcriptional activity. This hypothesis would be consistent with our published findings of up-regulated mRNA expression of eNOS, MnSOD, and catalase in SOD mimic-treated spermatozoa ex vivo (Otasevic et al., 2013). Therefore, SOD mimic modulates the redox environment via direct chromatin remodeling in spermatozoa. Fractal analysis has proven to be a good method for the analysis of chromatin condensation in human sperm nuclei. Ref: Otasevic V, Korac A, Vucetic M, Macanovic B, Garalejic E, Ivanovic-Burmazovic I, Filipovic MR, Buzadzic B, Stancic A, Jankovic A, Velickovic K, Golic I, Markelic M, Korac B. Is manganese (II) pentaazamacrocyclic superoxide dismutase mimic beneficial for human sperm mitochondria function and motility? Antioxid Redox Signal. 2013 Jan 10;18(2):170-8. doi: 10.1089/ars.2012.4684. Epub 2012 Jun 12. PMID: 22563824; PMCID: PMC3513981",
publisher = "Elsevier Inc.",
journal = "Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia",
title = "Fractal analysis of chromatin condensation in the human sperm nuclei",
doi = "10.1016/j.freeradbiomed.2021.08.178",
pages = "S114"
}

Golić, I., Aleksić, M., Kalezić, A., Janković, A., Korać, B.,& Korać, A.. (2021). Fractal analysis of chromatin condensation in the human sperm nuclei. in Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia
Elsevier Inc.., S114.
https://doi.org/10.1016/j.freeradbiomed.2021.08.178

Golić I, Aleksić M, Kalezić A, Janković A, Korać B, Korać A. Fractal analysis of chromatin condensation in the human sperm nuclei. in Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia. 2021;:S114.
doi:10.1016/j.freeradbiomed.2021.08.178 .

Golić, Igor, Aleksić, Marija, Kalezić, Anđelika, Janković, Aleksandra, Korać, Bato, Korać, Aleksandra, "Fractal analysis of chromatin condensation in the human sperm nuclei" in Free Radical Research Europe (SFRR-E) Annual Meeting Abstracts “Redox biology in the 21st century: a new scientific discipline” 15-18 June 2021, Belgrade, Serbia (2021):S114,
https://doi.org/10.1016/j.freeradbiomed.2021.08.178 . .

TY  - JOUR
AU  - Golić, Igor
AU  - Kalezić, Anđelika
AU  - Janković, Aleksandra
AU  - Jonić, Slavica
AU  - Korać, Bato
AU  - Korać, Aleksandra
PY  - 2020
UR  - www.mdpi.com/journal/ijms
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4077
AB  - The effects of insulin on the bioenergetic and thermogenic capacity of brown adipocyte mitochondria were investigated by focusing on key mitochondrial proteins. Two-month-old male Wistar rats were treated acutely or chronically with a low or high dose of insulin. Acute low insulin dose increased expression of all electron transport chain complexes and complex IV activity, whereas high dose increased complex II expression. Chronic low insulin dose decreased complex I and cyt c expression while increasing complex II and IV expression and complex IV activity. Chronic high insulin dose decreased complex II, III, cyt c, and increased complex IV expression. Uncoupling protein (UCP) 1 expression was decreased after acute high insulin but increased following chronic insulin treatment. ATP synthase expression was increased after acute and decreased after chronic insulin treatment. Only a high dose of insulin increased ATP synthase activity in acute and decreased it in chronic treatment. ATPase inhibitory factor protein expression was increased in all treated groups. Confocal microscopy showed that key mitochondrial proteins colocalize differently in different mitochondria within a single brown adipocyte, indicating mitochondrial mosaicism. These results suggest that insulin modulates the bioenergetic and thermogenic capacity of rat brown adipocytes in vivo by modulating mitochondrial mosaicism.
PB  - MDPI AG
T2  - International Journal of Molecular Sciences
T1  - Insulin modulates the bioenergetic and thermogenic capacity of rat brown adipocytes in vivo by modulating mitochondrial mosaicism
IS  - 23
VL  - 21
DO  - 10.3390/IJMS21239204
SP  - 1
EP  - 20
ER  - 

@article{
author = "Golić, Igor and Kalezić, Anđelika and Janković, Aleksandra and Jonić, Slavica and Korać, Bato and Korać, Aleksandra",
year = "2020",
abstract = "The effects of insulin on the bioenergetic and thermogenic capacity of brown adipocyte mitochondria were investigated by focusing on key mitochondrial proteins. Two-month-old male Wistar rats were treated acutely or chronically with a low or high dose of insulin. Acute low insulin dose increased expression of all electron transport chain complexes and complex IV activity, whereas high dose increased complex II expression. Chronic low insulin dose decreased complex I and cyt c expression while increasing complex II and IV expression and complex IV activity. Chronic high insulin dose decreased complex II, III, cyt c, and increased complex IV expression. Uncoupling protein (UCP) 1 expression was decreased after acute high insulin but increased following chronic insulin treatment. ATP synthase expression was increased after acute and decreased after chronic insulin treatment. Only a high dose of insulin increased ATP synthase activity in acute and decreased it in chronic treatment. ATPase inhibitory factor protein expression was increased in all treated groups. Confocal microscopy showed that key mitochondrial proteins colocalize differently in different mitochondria within a single brown adipocyte, indicating mitochondrial mosaicism. These results suggest that insulin modulates the bioenergetic and thermogenic capacity of rat brown adipocytes in vivo by modulating mitochondrial mosaicism.",
publisher = "MDPI AG",
journal = "International Journal of Molecular Sciences",
title = "Insulin modulates the bioenergetic and thermogenic capacity of rat brown adipocytes in vivo by modulating mitochondrial mosaicism",
number = "23",
volume = "21",
doi = "10.3390/IJMS21239204",
pages = "1-20"
}

Golić, I., Kalezić, A., Janković, A., Jonić, S., Korać, B.,& Korać, A.. (2020). Insulin modulates the bioenergetic and thermogenic capacity of rat brown adipocytes in vivo by modulating mitochondrial mosaicism. in International Journal of Molecular Sciences
MDPI AG., 21(23), 1-20.
https://doi.org/10.3390/IJMS21239204

Golić I, Kalezić A, Janković A, Jonić S, Korać B, Korać A. Insulin modulates the bioenergetic and thermogenic capacity of rat brown adipocytes in vivo by modulating mitochondrial mosaicism. in International Journal of Molecular Sciences. 2020;21(23):1-20.
doi:10.3390/IJMS21239204 .

Golić, Igor, Kalezić, Anđelika, Janković, Aleksandra, Jonić, Slavica, Korać, Bato, Korać, Aleksandra, "Insulin modulates the bioenergetic and thermogenic capacity of rat brown adipocytes in vivo by modulating mitochondrial mosaicism" in International Journal of Molecular Sciences, 21, no. 23 (2020):1-20,
https://doi.org/10.3390/IJMS21239204 . .

TY  - JOUR
AU  - Maksimović-Ivanić, Danijela
AU  - Bulatović, Mirna
AU  - Edeler, David
AU  - Bensing, Christian
AU  - Golić, Igor
AU  - Korać, Aleksandra
AU  - Kaluđerović, Goran N.
AU  - Mijatović, Sanja
PY  - 2019
UR  - http://link.springer.com/10.1007/s00775-019-01640-x
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3276
AB  - Extraordinary progress in medicinal inorganic chemistry in the past few years led to the rational design of novel platinum compounds, as well as nonplatinum metal-based antitumor agents, including organotin compounds, whose activity is not based on unrepairable interaction with DNA. To overcome poor solubility and toxicity problems that limited the application of these compounds numerous delivering systems were used (Lila et al. in Biol Pharm Bull 37:206–211, 2014; Yue and Cao in Curr Cancer Drug Targets 16:480–488, 2016; Duan et al. in WIREs Nanomed Nanobiotechnol 8:776–791, 2016). Regarding high drug loading capacity, mesoporous silica nanoparticles like SBA-15 became more important for targeted drug delivery. In this study, cellular uptake and biological activities responsible for organotin(IV) compound Ph3Sn(CH2)6OH (Sn6) grafted into (3-chloropropyl)triethoxysilane functionalized SBA-15 (SBA-15p → SBA-15p|Sn6) were evaluated in human melanoma A375 cell line. Moreover, the influence of SBA-15p grafted with organotin(IV) compound on the stemness of A375 cell was tested. Given the fact that SBA-15p|Sn6 nanoparticles are nonspherical and relatively large, their internalization efficiently started even after 15 min with stable adhesion to the cell membrane. After only 2 h of incubation of A375 cells with SBA-15p|Sn6 passive fluid-phase uptake and macropinocytosis were observed. Inside of the cell, treatment with SBA-15p loaded with Sn6 promoted caspase-dependent apoptosis in parallel with senescence development. The subpopulation of cells expressing Schwann-like phenotype arose upon the treatment, while the signaling pathway responsible for maintenance of pluripotency and invasiveness, Wnt, Notch1, and Oct3/4 were modulated towards less aggressive signature. In summary, SBA-15p enhances the efficacy of free Sn6 compound through efficient uptake and well profiled intracellular response followed with decreased stem characteristics of highly invasive A375 melanoma cells.
T2  - JBIC Journal of Biological Inorganic Chemistry
T1  - The interaction between SBA-15 derivative loaded with Ph3Sn(CH2)6OH and human melanoma A375 cell line: uptake and stem phenotype loss
DO  - 10.1007/s00775-019-01640-x
ER  - 

@article{
author = "Maksimović-Ivanić, Danijela and Bulatović, Mirna and Edeler, David and Bensing, Christian and Golić, Igor and Korać, Aleksandra and Kaluđerović, Goran N. and Mijatović, Sanja",
year = "2019",
abstract = "Extraordinary progress in medicinal inorganic chemistry in the past few years led to the rational design of novel platinum compounds, as well as nonplatinum metal-based antitumor agents, including organotin compounds, whose activity is not based on unrepairable interaction with DNA. To overcome poor solubility and toxicity problems that limited the application of these compounds numerous delivering systems were used (Lila et al. in Biol Pharm Bull 37:206–211, 2014; Yue and Cao in Curr Cancer Drug Targets 16:480–488, 2016; Duan et al. in WIREs Nanomed Nanobiotechnol 8:776–791, 2016). Regarding high drug loading capacity, mesoporous silica nanoparticles like SBA-15 became more important for targeted drug delivery. In this study, cellular uptake and biological activities responsible for organotin(IV) compound Ph3Sn(CH2)6OH (Sn6) grafted into (3-chloropropyl)triethoxysilane functionalized SBA-15 (SBA-15p → SBA-15p|Sn6) were evaluated in human melanoma A375 cell line. Moreover, the influence of SBA-15p grafted with organotin(IV) compound on the stemness of A375 cell was tested. Given the fact that SBA-15p|Sn6 nanoparticles are nonspherical and relatively large, their internalization efficiently started even after 15 min with stable adhesion to the cell membrane. After only 2 h of incubation of A375 cells with SBA-15p|Sn6 passive fluid-phase uptake and macropinocytosis were observed. Inside of the cell, treatment with SBA-15p loaded with Sn6 promoted caspase-dependent apoptosis in parallel with senescence development. The subpopulation of cells expressing Schwann-like phenotype arose upon the treatment, while the signaling pathway responsible for maintenance of pluripotency and invasiveness, Wnt, Notch1, and Oct3/4 were modulated towards less aggressive signature. In summary, SBA-15p enhances the efficacy of free Sn6 compound through efficient uptake and well profiled intracellular response followed with decreased stem characteristics of highly invasive A375 melanoma cells.",
journal = "JBIC Journal of Biological Inorganic Chemistry",
title = "The interaction between SBA-15 derivative loaded with Ph3Sn(CH2)6OH and human melanoma A375 cell line: uptake and stem phenotype loss",
doi = "10.1007/s00775-019-01640-x"
}

Maksimović-Ivanić, D., Bulatović, M., Edeler, D., Bensing, C., Golić, I., Korać, A., Kaluđerović, G. N.,& Mijatović, S.. (2019). The interaction between SBA-15 derivative loaded with Ph3Sn(CH2)6OH and human melanoma A375 cell line: uptake and stem phenotype loss. in JBIC Journal of Biological Inorganic Chemistry.
https://doi.org/10.1007/s00775-019-01640-x

Maksimović-Ivanić D, Bulatović M, Edeler D, Bensing C, Golić I, Korać A, Kaluđerović GN, Mijatović S. The interaction between SBA-15 derivative loaded with Ph3Sn(CH2)6OH and human melanoma A375 cell line: uptake and stem phenotype loss. in JBIC Journal of Biological Inorganic Chemistry. 2019;.
doi:10.1007/s00775-019-01640-x .

Maksimović-Ivanić, Danijela, Bulatović, Mirna, Edeler, David, Bensing, Christian, Golić, Igor, Korać, Aleksandra, Kaluđerović, Goran N., Mijatović, Sanja, "The interaction between SBA-15 derivative loaded with Ph3Sn(CH2)6OH and human melanoma A375 cell line: uptake and stem phenotype loss" in JBIC Journal of Biological Inorganic Chemistry (2019),
https://doi.org/10.1007/s00775-019-01640-x . .

TY  - JOUR
AU  - Petrović, Anja
AU  - Bogojević, Desanka
AU  - Korać, Aleksandra
AU  - Golić, Igor
AU  - Jovanović Stojanov, Sofija
AU  - Martinović, Vesna
AU  - Ivanović Matić, Svetlana
AU  - Stevanović, Jelena
AU  - Poznanović, Goran
AU  - Grigorov, Ilijana
PY  - 2017
UR  - http://link.springer.com/10.1007/s13105-017-0574-0
UR  - http://www.ncbi.nlm.nih.gov/pubmed/28695466
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2787
AB  - The progression of oxidative stress, resulting cell damage, and cell death underlies the etiology of liver damage/dysfunction as a complication of diabetes. High-mobility group box 1 (HMGB1) protein, a chromatin-binding nuclear protein and damage-associated molecular pattern molecule, is integral to oxidative stress and signaling pathways regulating cell death and cell survival. We previously found that in streptozotocin (STZ)-induced diabetic rats, reduction of oxidative stress after melatonin administration lowered necrotic cell death and increased expression of HMGB1 and hepatocellular damage. In the present study, we examined whether alleviation of diabetes-attendant oxidative stress and ensuing change in HMGB1 expression influence the dynamic equilibrium between apoptosis/autophagy and liver damage. We observed that elevated HMGB1 protein levels in diabetic rat liver accompanied increased interactions of HMGB1 with TLR4 and RAGE, and activation of the intrinsic apoptotic pathway and Beclin 1-dependent autophagy. The absence of p62 degradation in diabetic rat liver pointed to defective autophagy which was responsible for lower autophagosome/autophagolysosome formation and an increased apoptosis/autophagy ratio. Compared to diabetic rats, in melatonin-treated diabetic rats, the structure of liver cells was preserved, HMGB1/TLR4 interaction and downstream apoptotic signaling were significantly reduced, HMGB1/Beclin 1 colocalization and interactions were augmented and Beclin 1-mediated autophagy, mithophagy in particular, were increased. We concluded that in mild oxidative stress, HMGB1 is cytoprotective, whereas in intense oxidative stress, HMGB1 actions promote cell death and liver damage. Since reduced HMGB1 binds to RAGE but not to TLR4, redox modification of HMGB1 as a mechanism regulating the cross-talk between apoptosis and autophagy in diabetes is discussed.
T2  - Journal of Physiology and Biochemistry
T1  - Oxidative stress-dependent contribution of HMGB1 to the interplay between apoptosis and autophagy in diabetic rat liver.
DO  - 10.1007/s13105-017-0574-0
ER  - 

@article{
author = "Petrović, Anja and Bogojević, Desanka and Korać, Aleksandra and Golić, Igor and Jovanović Stojanov, Sofija and Martinović, Vesna and Ivanović Matić, Svetlana and Stevanović, Jelena and Poznanović, Goran and Grigorov, Ilijana",
year = "2017",
abstract = "The progression of oxidative stress, resulting cell damage, and cell death underlies the etiology of liver damage/dysfunction as a complication of diabetes. High-mobility group box 1 (HMGB1) protein, a chromatin-binding nuclear protein and damage-associated molecular pattern molecule, is integral to oxidative stress and signaling pathways regulating cell death and cell survival. We previously found that in streptozotocin (STZ)-induced diabetic rats, reduction of oxidative stress after melatonin administration lowered necrotic cell death and increased expression of HMGB1 and hepatocellular damage. In the present study, we examined whether alleviation of diabetes-attendant oxidative stress and ensuing change in HMGB1 expression influence the dynamic equilibrium between apoptosis/autophagy and liver damage. We observed that elevated HMGB1 protein levels in diabetic rat liver accompanied increased interactions of HMGB1 with TLR4 and RAGE, and activation of the intrinsic apoptotic pathway and Beclin 1-dependent autophagy. The absence of p62 degradation in diabetic rat liver pointed to defective autophagy which was responsible for lower autophagosome/autophagolysosome formation and an increased apoptosis/autophagy ratio. Compared to diabetic rats, in melatonin-treated diabetic rats, the structure of liver cells was preserved, HMGB1/TLR4 interaction and downstream apoptotic signaling were significantly reduced, HMGB1/Beclin 1 colocalization and interactions were augmented and Beclin 1-mediated autophagy, mithophagy in particular, were increased. We concluded that in mild oxidative stress, HMGB1 is cytoprotective, whereas in intense oxidative stress, HMGB1 actions promote cell death and liver damage. Since reduced HMGB1 binds to RAGE but not to TLR4, redox modification of HMGB1 as a mechanism regulating the cross-talk between apoptosis and autophagy in diabetes is discussed.",
journal = "Journal of Physiology and Biochemistry",
title = "Oxidative stress-dependent contribution of HMGB1 to the interplay between apoptosis and autophagy in diabetic rat liver.",
doi = "10.1007/s13105-017-0574-0"
}

Petrović, A., Bogojević, D., Korać, A., Golić, I., Jovanović Stojanov, S., Martinović, V., Ivanović Matić, S., Stevanović, J., Poznanović, G.,& Grigorov, I.. (2017). Oxidative stress-dependent contribution of HMGB1 to the interplay between apoptosis and autophagy in diabetic rat liver.. in Journal of Physiology and Biochemistry.
https://doi.org/10.1007/s13105-017-0574-0

Petrović A, Bogojević D, Korać A, Golić I, Jovanović Stojanov S, Martinović V, Ivanović Matić S, Stevanović J, Poznanović G, Grigorov I. Oxidative stress-dependent contribution of HMGB1 to the interplay between apoptosis and autophagy in diabetic rat liver.. in Journal of Physiology and Biochemistry. 2017;.
doi:10.1007/s13105-017-0574-0 .

Petrović, Anja, Bogojević, Desanka, Korać, Aleksandra, Golić, Igor, Jovanović Stojanov, Sofija, Martinović, Vesna, Ivanović Matić, Svetlana, Stevanović, Jelena, Poznanović, Goran, Grigorov, Ilijana, "Oxidative stress-dependent contribution of HMGB1 to the interplay between apoptosis and autophagy in diabetic rat liver." in Journal of Physiology and Biochemistry (2017),
https://doi.org/10.1007/s13105-017-0574-0 . .

TY  - JOUR
AU  - Otašević, Vesna
AU  - Šurlan, Lela
AU  - Vučetić, Milica
AU  - Tulić, Ivan
AU  - Buzadžić, Biljana
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Veličković, Ksenija
AU  - Golić, Igor
AU  - Markelić, Milica
AU  - Korać, Aleksandra
AU  - Korać, Bato
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6213
AB  - Developmental dysfunction in embryos, such as a lethal level of fragmentation, is assumed to be mitochondrial in origin. This study investigated the molecular basis of mitochondrial impairment in embryo fragmentation. Transcription patterns of factors that determine mitochondrial functionality: (i) components of the oxidative phosphorylation (OXPHOS) - complex I, cytochrome b, complex IV and ATP synthase; (ii) mitochondrial membrane potential (MMP); (iii) mitochondrial DNA (mtDNA) content and (iv) proteins involved in mitochondrial dynamics, mitofusin 1 (Mfn1) and dynamin related protein 1 (Drp1) were examined in six-cells Day 3 non-fragmented (control), low-fragmented (LF) and high-fragmented (HF) human embryos. Gene expression of mitochondria-encoded components of complex I and IV, cytochrome b and mtDNA were increased in HF embryos compared with control and LF embryos. In LF embryos, expression of these molecules was decreased compared with control and HF embryos. Both classes of fragmented embryos had decreased MMP compared with control. LF embryos had increased gene expression of Mfn1 accompanied by decreased expression of Drp1, while HF embryos had decreased Mfn1 expression but increased Drp1 expression. The study revealed that each improper transcriptional (in)activation of mitochondria-encoded components of the OXPHOS during early in vitro embryo development is associated with a decrease in MMP and with embryo fragmentation. The results also showed the importance of mitochondrial dynamics in fragmentation, at least in the extent of this process.
PB  - CSIRO Publishing
T2  - Reproduction, Fertility and Development
T1  - Expression patterns of mitochondrial OXPHOS components, mitofusin 1 and dynamin-related protein 1 are associated with human embryo fragmentation
IS  - 3
VL  - 28
DO  - 10.1071/RD13415
SP  - 319
EP  - 327
ER  - 

@article{
author = "Otašević, Vesna and Šurlan, Lela and Vučetić, Milica and Tulić, Ivan and Buzadžić, Biljana and Stančić, Ana and Janković, Aleksandra and Veličković, Ksenija and Golić, Igor and Markelić, Milica and Korać, Aleksandra and Korać, Bato",
year = "2016",
abstract = "Developmental dysfunction in embryos, such as a lethal level of fragmentation, is assumed to be mitochondrial in origin. This study investigated the molecular basis of mitochondrial impairment in embryo fragmentation. Transcription patterns of factors that determine mitochondrial functionality: (i) components of the oxidative phosphorylation (OXPHOS) - complex I, cytochrome b, complex IV and ATP synthase; (ii) mitochondrial membrane potential (MMP); (iii) mitochondrial DNA (mtDNA) content and (iv) proteins involved in mitochondrial dynamics, mitofusin 1 (Mfn1) and dynamin related protein 1 (Drp1) were examined in six-cells Day 3 non-fragmented (control), low-fragmented (LF) and high-fragmented (HF) human embryos. Gene expression of mitochondria-encoded components of complex I and IV, cytochrome b and mtDNA were increased in HF embryos compared with control and LF embryos. In LF embryos, expression of these molecules was decreased compared with control and HF embryos. Both classes of fragmented embryos had decreased MMP compared with control. LF embryos had increased gene expression of Mfn1 accompanied by decreased expression of Drp1, while HF embryos had decreased Mfn1 expression but increased Drp1 expression. The study revealed that each improper transcriptional (in)activation of mitochondria-encoded components of the OXPHOS during early in vitro embryo development is associated with a decrease in MMP and with embryo fragmentation. The results also showed the importance of mitochondrial dynamics in fragmentation, at least in the extent of this process.",
publisher = "CSIRO Publishing",
journal = "Reproduction, Fertility and Development",
title = "Expression patterns of mitochondrial OXPHOS components, mitofusin 1 and dynamin-related protein 1 are associated with human embryo fragmentation",
number = "3",
volume = "28",
doi = "10.1071/RD13415",
pages = "319-327"
}

Otašević, V., Šurlan, L., Vučetić, M., Tulić, I., Buzadžić, B., Stančić, A., Janković, A., Veličković, K., Golić, I., Markelić, M., Korać, A.,& Korać, B.. (2016). Expression patterns of mitochondrial OXPHOS components, mitofusin 1 and dynamin-related protein 1 are associated with human embryo fragmentation. in Reproduction, Fertility and Development
CSIRO Publishing., 28(3), 319-327.
https://doi.org/10.1071/RD13415

Otašević V, Šurlan L, Vučetić M, Tulić I, Buzadžić B, Stančić A, Janković A, Veličković K, Golić I, Markelić M, Korać A, Korać B. Expression patterns of mitochondrial OXPHOS components, mitofusin 1 and dynamin-related protein 1 are associated with human embryo fragmentation. in Reproduction, Fertility and Development. 2016;28(3):319-327.
doi:10.1071/RD13415 .

Otašević, Vesna, Šurlan, Lela, Vučetić, Milica, Tulić, Ivan, Buzadžić, Biljana, Stančić, Ana, Janković, Aleksandra, Veličković, Ksenija, Golić, Igor, Markelić, Milica, Korać, Aleksandra, Korać, Bato, "Expression patterns of mitochondrial OXPHOS components, mitofusin 1 and dynamin-related protein 1 are associated with human embryo fragmentation" in Reproduction, Fertility and Development, 28, no. 3 (2016):319-327,
https://doi.org/10.1071/RD13415 . .

TY  - CONF
AU  - Surlan, L
AU  - Otašević, Vesna
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Vučetić, Milica
AU  - Stanković, V
AU  - Stojnić, J
AU  - Radunović, Nebojsa V
AU  - Tulić, Ivan
AU  - Korać, Bato
AU  - Korac, Aleksandra B
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1002
C3  - Human Reproduction
T1  - Exosomes as bioactive messengers in a critical time for preimplantation embryos n an ultrastructural study
IS  - null
VL  - 28
SP  - 69
EP  - 186
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1002
ER  - 

@conference{
author = "Surlan, L and Otašević, Vesna and Velicković, Ksenija D and Golić, Igor and Vučetić, Milica and Stanković, V and Stojnić, J and Radunović, Nebojsa V and Tulić, Ivan and Korać, Bato and Korac, Aleksandra B",
year = "2013",
journal = "Human Reproduction",
title = "Exosomes as bioactive messengers in a critical time for preimplantation embryos n an ultrastructural study",
number = "null",
volume = "28",
pages = "69-186",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1002"
}

Surlan, L., Otašević, V., Velicković, K. D., Golić, I., Vučetić, M., Stanković, V., Stojnić, J., Radunović, N. V., Tulić, I., Korać, B.,& Korac, A. B.. (2013). Exosomes as bioactive messengers in a critical time for preimplantation embryos n an ultrastructural study. in Human Reproduction, 28(null), 69-186.
https://hdl.handle.net/21.15107/rcub_ibiss_1002

Surlan L, Otašević V, Velicković KD, Golić I, Vučetić M, Stanković V, Stojnić J, Radunović NV, Tulić I, Korać B, Korac AB. Exosomes as bioactive messengers in a critical time for preimplantation embryos n an ultrastructural study. in Human Reproduction. 2013;28(null):69-186.
https://hdl.handle.net/21.15107/rcub_ibiss_1002 .

Surlan, L, Otašević, Vesna, Velicković, Ksenija D, Golić, Igor, Vučetić, Milica, Stanković, V, Stojnić, J, Radunović, Nebojsa V, Tulić, Ivan, Korać, Bato, Korac, Aleksandra B, "Exosomes as bioactive messengers in a critical time for preimplantation embryos n an ultrastructural study" in Human Reproduction, 28, no. null (2013):69-186,
https://hdl.handle.net/21.15107/rcub_ibiss_1002 .

TY  - JOUR
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Klepal, Waltraud
AU  - Otašević, Vesna
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Buzadžić, Biljana J.
AU  - Korać, Bato
AU  - Korac, Aleksandra B
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1030
AB  - The aim of this study was to investigate lipofuscin origin in brown adipocytes of hyperinsulinaemic rats and the possible role of lipid peroxidation and iron in this process. Ultrastructural examination revealed hyperinsulinaemia-induced enhancement in the lipofuscin production, accompanied by an increase of mitochondrial damage in brown adipocytes. Extensive fusions of lipid droplets and mitochondria with lysosomes were also observed. Confocal microscopy showed lipofuscin autofluorescence emission in brown adipose tissue (BAT) after excitation at 488 nm and 633 nm, particularly in the insulin-treated groups. The presence and distribution of lipid peroxidation product, 4-hydroxy-2-nonenal (4-HNE), in brown adipocytes was assessed by immunohistochemical examination revealing its higher content after treatment with insulin. The iron content was quantified by electron dispersive X-ray analysis (EDX) showing its higher content in the hyperinsulinaemic groups. The ultrastucture of the majority of lipofuscin granules suggests their mitochondrial origin, which was additionally confirmed by their co-localization with ATP synthase. In conclusion, our results suggest that increased lipofuscinogenesis in the brown adipocytes of hyperinsulinaemic rats is a consequence of lipid peroxidation, mitochondrial damage and iron accumulation.
T2  - Histology and Histopathology
T1  - The origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: the role of lipid peroxidation and iron
IS  - 4
VL  - 28
SP  - 435
EP  - 503
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1030
ER  - 

@article{
author = "Markelić, Milica B and Velicković, Ksenija D and Golić, Igor and Klepal, Waltraud and Otašević, Vesna and Stančić, Ana and Janković, Aleksandra and Vučetić, Milica and Buzadžić, Biljana J. and Korać, Bato and Korac, Aleksandra B",
year = "2013",
abstract = "The aim of this study was to investigate lipofuscin origin in brown adipocytes of hyperinsulinaemic rats and the possible role of lipid peroxidation and iron in this process. Ultrastructural examination revealed hyperinsulinaemia-induced enhancement in the lipofuscin production, accompanied by an increase of mitochondrial damage in brown adipocytes. Extensive fusions of lipid droplets and mitochondria with lysosomes were also observed. Confocal microscopy showed lipofuscin autofluorescence emission in brown adipose tissue (BAT) after excitation at 488 nm and 633 nm, particularly in the insulin-treated groups. The presence and distribution of lipid peroxidation product, 4-hydroxy-2-nonenal (4-HNE), in brown adipocytes was assessed by immunohistochemical examination revealing its higher content after treatment with insulin. The iron content was quantified by electron dispersive X-ray analysis (EDX) showing its higher content in the hyperinsulinaemic groups. The ultrastucture of the majority of lipofuscin granules suggests their mitochondrial origin, which was additionally confirmed by their co-localization with ATP synthase. In conclusion, our results suggest that increased lipofuscinogenesis in the brown adipocytes of hyperinsulinaemic rats is a consequence of lipid peroxidation, mitochondrial damage and iron accumulation.",
journal = "Histology and Histopathology",
title = "The origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: the role of lipid peroxidation and iron",
number = "4",
volume = "28",
pages = "435-503",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1030"
}

Markelić, M. B., Velicković, K. D., Golić, I., Klepal, W., Otašević, V., Stančić, A., Janković, A., Vučetić, M., Buzadžić, B. J., Korać, B.,& Korac, A. B.. (2013). The origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: the role of lipid peroxidation and iron. in Histology and Histopathology, 28(4), 435-503.
https://hdl.handle.net/21.15107/rcub_ibiss_1030

Markelić MB, Velicković KD, Golić I, Klepal W, Otašević V, Stančić A, Janković A, Vučetić M, Buzadžić BJ, Korać B, Korac AB. The origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: the role of lipid peroxidation and iron. in Histology and Histopathology. 2013;28(4):435-503.
https://hdl.handle.net/21.15107/rcub_ibiss_1030 .

Markelić, Milica B, Velicković, Ksenija D, Golić, Igor, Klepal, Waltraud, Otašević, Vesna, Stančić, Ana, Janković, Aleksandra, Vučetić, Milica, Buzadžić, Biljana J., Korać, Bato, Korac, Aleksandra B, "The origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: the role of lipid peroxidation and iron" in Histology and Histopathology, 28, no. 4 (2013):435-503,
https://hdl.handle.net/21.15107/rcub_ibiss_1030 .

TY  - JOUR
AU  - Otašević, Vesna
AU  - Korac, Aleksandra B
AU  - Vučetić, Milica
AU  - Macanović, Biljana
AU  - Garalejić, Eliana
AU  - Ivanović-Burmazović, Ivana S
AU  - Filipović, Milos R
AU  - Buzadžić, Biljana J.
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Markelić, Milica B
AU  - Korać, Bato
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1067
AB  - Mitochondria play an important role in sperm cell maturation and function. Here, we examined whether (and how) changes in sperm redox milieu affect the functional status of sperm mitochondria, that is, sperm functionality. Compared with the control, incubation in Tyrode's medium for 3 h, under noncapacitating conditions, decreased sperm motility, the amount of nitric oxide ((NO)-N-center dot), the number of MitoTracker (R) Green FM (MT-G) positive mitochondria, and the expression of complexes I and IV of the mitochondrial respiratory chain. In turn, superoxide dismutase (SOD) mimic (M40403) treatment restored/increased these parameters, as well as the expression of endothelial nitric oxide synthase, manganese SOD, and catalase. These data lead to the hypothesis that M40403 improves mitochondrial functional state and motility of spermatozoa, as well as (NO)-N-center dot might be involved in the observed effects of the mimic. Antioxid. Redox Signal. 18, 170-178.
T2  - Antioxidants & Redox Signaling
T1  - Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?
IS  - 2
VL  - 18
SP  - 23
EP  - 178
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1067
ER  - 

@article{
author = "Otašević, Vesna and Korac, Aleksandra B and Vučetić, Milica and Macanović, Biljana and Garalejić, Eliana and Ivanović-Burmazović, Ivana S and Filipović, Milos R and Buzadžić, Biljana J. and Stančić, Ana and Janković, Aleksandra and Velicković, Ksenija D and Golić, Igor and Markelić, Milica B and Korać, Bato",
year = "2013",
abstract = "Mitochondria play an important role in sperm cell maturation and function. Here, we examined whether (and how) changes in sperm redox milieu affect the functional status of sperm mitochondria, that is, sperm functionality. Compared with the control, incubation in Tyrode's medium for 3 h, under noncapacitating conditions, decreased sperm motility, the amount of nitric oxide ((NO)-N-center dot), the number of MitoTracker (R) Green FM (MT-G) positive mitochondria, and the expression of complexes I and IV of the mitochondrial respiratory chain. In turn, superoxide dismutase (SOD) mimic (M40403) treatment restored/increased these parameters, as well as the expression of endothelial nitric oxide synthase, manganese SOD, and catalase. These data lead to the hypothesis that M40403 improves mitochondrial functional state and motility of spermatozoa, as well as (NO)-N-center dot might be involved in the observed effects of the mimic. Antioxid. Redox Signal. 18, 170-178.",
journal = "Antioxidants & Redox Signaling",
title = "Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?",
number = "2",
volume = "18",
pages = "23-178",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1067"
}

Otašević, V., Korac, A. B., Vučetić, M., Macanović, B., Garalejić, E., Ivanović-Burmazović, I. S., Filipović, M. R., Buzadžić, B. J., Stančić, A., Janković, A., Velicković, K. D., Golić, I., Markelić, M. B.,& Korać, B.. (2013). Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?. in Antioxidants & Redox Signaling, 18(2), 23-178.
https://hdl.handle.net/21.15107/rcub_ibiss_1067

Otašević V, Korac AB, Vučetić M, Macanović B, Garalejić E, Ivanović-Burmazović IS, Filipović MR, Buzadžić BJ, Stančić A, Janković A, Velicković KD, Golić I, Markelić MB, Korać B. Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?. in Antioxidants & Redox Signaling. 2013;18(2):23-178.
https://hdl.handle.net/21.15107/rcub_ibiss_1067 .

Otašević, Vesna, Korac, Aleksandra B, Vučetić, Milica, Macanović, Biljana, Garalejić, Eliana, Ivanović-Burmazović, Ivana S, Filipović, Milos R, Buzadžić, Biljana J., Stančić, Ana, Janković, Aleksandra, Velicković, Ksenija D, Golić, Igor, Markelić, Milica B, Korać, Bato, "Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?" in Antioxidants & Redox Signaling, 18, no. 2 (2013):23-178,
https://hdl.handle.net/21.15107/rcub_ibiss_1067 .

TY  - JOUR
AU  - Stančić, Ana
AU  - Otašević, Vesna
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Ivanović-Burmazović, Ivana S
AU  - Filipović, Milos R
AU  - Korac, Aleksandra B
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Buzadžić, Biljana J.
AU  - Korać, Bato
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/960
AB  - Hippocampal structural changes associated with diabetes-related cognitive impairments are well described, but their molecular background remained vague. We examined whether/how diabetes alters molecular basis of energy metabolism in hippocampus readily after diabetes onset, with special emphasis on its redox-sensitivity. To induce diabetes, adult Mill Hill hybrid hooded rats received a single alloxan dose (120 mg/kg). Both non-diabetic and diabetic groups were further divided in two subgroups receiving (i) or not (ii) superoxide dismutase (SOD) mimic, [Mn(II)(pyane)Cl-2] for 7 days, i.p. Treatment of the diabetic animals started after blood glucose level >= 12 mM. Diabetes decreased protein levels of oxidative phosphorylation components: complex III and ATP synthase. In contrast, protein amounts of glyceraldehyde-3-phosphate dehydrogenase, pyruvate dehydrogenase, and hypoxia-inducible factor-1 alpha - the key regulator of energy metabolism in stress conditions, were higher in diabetic animals. Treatment with SOD mimic restored/increased the levels of oxidative phosphorylation components and returned hypoxia-inducible factor-1 alpha to control level, while diabetes-induced up-regulation of glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase, was additionally stimulated. To conclude, our results provide insight into the earliest molecular changes of energy-producing pathways in diabetes that may account for structural/functional disturbance of hippocampus, seen during disease progression. Also, data suggest [Mn(II)(pyane)Cl-2] as potential therapeutic agent in cutting-edge approaches to threat this widespread metabolic disorder. (C) 2013 Elsevier Inc. All rights reserved.
T2  - Brain Research Bulletin
T1  - Molecular basis of hippocampal energy metabolism in diabetic rats: The effects of SOD mimic
IS  - null
VL  - 99
SP  - 153
EP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_960
ER  - 

@article{
author = "Stančić, Ana and Otašević, Vesna and Janković, Aleksandra and Vučetić, Milica and Ivanović-Burmazović, Ivana S and Filipović, Milos R and Korac, Aleksandra B and Markelić, Milica B and Velicković, Ksenija D and Golić, Igor and Buzadžić, Biljana J. and Korać, Bato",
year = "2013",
abstract = "Hippocampal structural changes associated with diabetes-related cognitive impairments are well described, but their molecular background remained vague. We examined whether/how diabetes alters molecular basis of energy metabolism in hippocampus readily after diabetes onset, with special emphasis on its redox-sensitivity. To induce diabetes, adult Mill Hill hybrid hooded rats received a single alloxan dose (120 mg/kg). Both non-diabetic and diabetic groups were further divided in two subgroups receiving (i) or not (ii) superoxide dismutase (SOD) mimic, [Mn(II)(pyane)Cl-2] for 7 days, i.p. Treatment of the diabetic animals started after blood glucose level >= 12 mM. Diabetes decreased protein levels of oxidative phosphorylation components: complex III and ATP synthase. In contrast, protein amounts of glyceraldehyde-3-phosphate dehydrogenase, pyruvate dehydrogenase, and hypoxia-inducible factor-1 alpha - the key regulator of energy metabolism in stress conditions, were higher in diabetic animals. Treatment with SOD mimic restored/increased the levels of oxidative phosphorylation components and returned hypoxia-inducible factor-1 alpha to control level, while diabetes-induced up-regulation of glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase, was additionally stimulated. To conclude, our results provide insight into the earliest molecular changes of energy-producing pathways in diabetes that may account for structural/functional disturbance of hippocampus, seen during disease progression. Also, data suggest [Mn(II)(pyane)Cl-2] as potential therapeutic agent in cutting-edge approaches to threat this widespread metabolic disorder. (C) 2013 Elsevier Inc. All rights reserved.",
journal = "Brain Research Bulletin",
title = "Molecular basis of hippocampal energy metabolism in diabetic rats: The effects of SOD mimic",
number = "null",
volume = "99",
pages = "153-33",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_960"
}

Stančić, A., Otašević, V., Janković, A., Vučetić, M., Ivanović-Burmazović, I. S., Filipović, M. R., Korac, A. B., Markelić, M. B., Velicković, K. D., Golić, I., Buzadžić, B. J.,& Korać, B.. (2013). Molecular basis of hippocampal energy metabolism in diabetic rats: The effects of SOD mimic. in Brain Research Bulletin, 99(null), 153-33.
https://hdl.handle.net/21.15107/rcub_ibiss_960

Stančić A, Otašević V, Janković A, Vučetić M, Ivanović-Burmazović IS, Filipović MR, Korac AB, Markelić MB, Velicković KD, Golić I, Buzadžić BJ, Korać B. Molecular basis of hippocampal energy metabolism in diabetic rats: The effects of SOD mimic. in Brain Research Bulletin. 2013;99(null):153-33.
https://hdl.handle.net/21.15107/rcub_ibiss_960 .

Stančić, Ana, Otašević, Vesna, Janković, Aleksandra, Vučetić, Milica, Ivanović-Burmazović, Ivana S, Filipović, Milos R, Korac, Aleksandra B, Markelić, Milica B, Velicković, Ksenija D, Golić, Igor, Buzadžić, Biljana J., Korać, Bato, "Molecular basis of hippocampal energy metabolism in diabetic rats: The effects of SOD mimic" in Brain Research Bulletin, 99, no. null (2013):153-33,
https://hdl.handle.net/21.15107/rcub_ibiss_960 .

TY  - JOUR
AU  - Stančić, Ana
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
AU  - Otašević, Vesna
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Korać, Bato
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/951
AB  - This study examined the molecular basis of energy-related regulatory mechanisms underlying metabolic recruitment of skeletal muscle during cold acclimation and possible involvement of the L-arginine/nitric oxide-producing pathway. Rats exposed to cold (4 +/- 1 degrees C) for periods of 1, 3, 7, 12, 21 and 45 days were divided into three groups: untreated, L-arginine treated and N-omega-nitro-L-arginine methyl ester (L-NAME) treated. Compared with controls (22 +/- 1 degrees C), there was an initial increase in the protein level of 5'-AMP-activated protein kinase alpha (day 1), followed by an increase in peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1 alpha) and peroxisome proliferator-activated receptors (PPARs): PPAR alpha and PPAR gamma from day 1 and PPAR delta from day 7 of cold acclimation. Activation of the PGC-1 alpha/PPAR transcription program was accompanied by increased protein expression of the key metabolic enzymes in beta-oxidation, the tricarboxylic acid cycle and oxidative phosphorylation, with the exceptions in complex I (no changes) and ATP synthase (decreased at day 1). Cold did not affect hexokinase and GAPDH protein levels, but increased lactate dehydrogenase activity compared with controls (1-45 days). L-arginine sustained, accelerated and/or intensified cold-induced molecular remodeling throughout cold acclimation. L-NAME exerted phase-dependent effects: similar to L-arginine in early cold acclimation and opposite after prolonged cold exposure (from day 21). It seems that upregulation of the PGC-1 alpha/PPAR transcription program early during cold acclimation triggers the molecular recruitment of skeletal muscle underlying the shift to more oxidative metabolism during prolonged cold acclimation. Our results suggest that nitric oxide has a role in maintaining the skeletal muscle oxidative phenotype in late cold acclimation but question its role early in cold acclimation.
T2  - Journal of Experimental Biology
T1  - Regulatory role of PGC-1 alpha/PPAR signaling in skeletal muscle metabolic recruitment during cold acclimation
IS  - 22
VL  - 216
SP  - 73
EP  - 4241
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_951
ER  - 

@article{
author = "Stančić, Ana and Buzadžić, Biljana J. and Korac, Aleksandra B and Otašević, Vesna and Janković, Aleksandra and Vučetić, Milica and Markelić, Milica B and Velicković, Ksenija D and Golić, Igor and Korać, Bato",
year = "2013",
abstract = "This study examined the molecular basis of energy-related regulatory mechanisms underlying metabolic recruitment of skeletal muscle during cold acclimation and possible involvement of the L-arginine/nitric oxide-producing pathway. Rats exposed to cold (4 +/- 1 degrees C) for periods of 1, 3, 7, 12, 21 and 45 days were divided into three groups: untreated, L-arginine treated and N-omega-nitro-L-arginine methyl ester (L-NAME) treated. Compared with controls (22 +/- 1 degrees C), there was an initial increase in the protein level of 5'-AMP-activated protein kinase alpha (day 1), followed by an increase in peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1 alpha) and peroxisome proliferator-activated receptors (PPARs): PPAR alpha and PPAR gamma from day 1 and PPAR delta from day 7 of cold acclimation. Activation of the PGC-1 alpha/PPAR transcription program was accompanied by increased protein expression of the key metabolic enzymes in beta-oxidation, the tricarboxylic acid cycle and oxidative phosphorylation, with the exceptions in complex I (no changes) and ATP synthase (decreased at day 1). Cold did not affect hexokinase and GAPDH protein levels, but increased lactate dehydrogenase activity compared with controls (1-45 days). L-arginine sustained, accelerated and/or intensified cold-induced molecular remodeling throughout cold acclimation. L-NAME exerted phase-dependent effects: similar to L-arginine in early cold acclimation and opposite after prolonged cold exposure (from day 21). It seems that upregulation of the PGC-1 alpha/PPAR transcription program early during cold acclimation triggers the molecular recruitment of skeletal muscle underlying the shift to more oxidative metabolism during prolonged cold acclimation. Our results suggest that nitric oxide has a role in maintaining the skeletal muscle oxidative phenotype in late cold acclimation but question its role early in cold acclimation.",
journal = "Journal of Experimental Biology",
title = "Regulatory role of PGC-1 alpha/PPAR signaling in skeletal muscle metabolic recruitment during cold acclimation",
number = "22",
volume = "216",
pages = "73-4241",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_951"
}

Stančić, A., Buzadžić, B. J., Korac, A. B., Otašević, V., Janković, A., Vučetić, M., Markelić, M. B., Velicković, K. D., Golić, I.,& Korać, B.. (2013). Regulatory role of PGC-1 alpha/PPAR signaling in skeletal muscle metabolic recruitment during cold acclimation. in Journal of Experimental Biology, 216(22), 73-4241.
https://hdl.handle.net/21.15107/rcub_ibiss_951

Stančić A, Buzadžić BJ, Korac AB, Otašević V, Janković A, Vučetić M, Markelić MB, Velicković KD, Golić I, Korać B. Regulatory role of PGC-1 alpha/PPAR signaling in skeletal muscle metabolic recruitment during cold acclimation. in Journal of Experimental Biology. 2013;216(22):73-4241.
https://hdl.handle.net/21.15107/rcub_ibiss_951 .

Stančić, Ana, Buzadžić, Biljana J., Korac, Aleksandra B, Otašević, Vesna, Janković, Aleksandra, Vučetić, Milica, Markelić, Milica B, Velicković, Ksenija D, Golić, Igor, Korać, Bato, "Regulatory role of PGC-1 alpha/PPAR signaling in skeletal muscle metabolic recruitment during cold acclimation" in Journal of Experimental Biology, 216, no. 22 (2013):73-4241,
https://hdl.handle.net/21.15107/rcub_ibiss_951 .

TY  - JOUR
AU  - Vučetić, Milica
AU  - Stančić, Ana
AU  - Otašević, Vesna
AU  - Janković, Aleksandra
AU  - Korac, Aleksandra B
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Buzadžić, Biljana J.
AU  - Storey, Kenneth B
AU  - Korać, Bato
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/944
AB  - Any alteration in oxidative metabolism is coupled with a corresponding response by an antioxidant defense (AD) in appropriate subcellular compartments. Seasonal hibernators pass through circannual metabolic adaptations that allow them to either maintain euthermy (cold acclimation) or enter winter torpor with body temperature falling to low values. The present study aimed to investigate the corresponding pattern of AD enzyme protein expressions associated with these strategies in the main tissues involved in whole animal energy homeostasis: brown and white adipose tissues (BAT and WAT, respectively), liver, and skeletal muscle. European ground squirrels (Spermophilus citellus) were exposed to low temperature (4 +/- 1 C) and then divided into two groups: (1) animals fell into torpor (hibernating group) and (2) animals stayed active and euthermic for 1, 3, 7, 12, or 21 days (cold-exposed group). We examined the effects of cold acclimation and hibernation on the tissue-dependent protein expression of four enzymes which catalyze the two-step detoxification of superoxide to water: superoxide dismutase 1 and 2 (SOD 1 and 2), catalase (CAT), and glutathione peroxidase (GSH-Px). The results showed that hibernation induced an increase of AD enzyme protein expressions in BAT and skeletal muscle. However, AD enzyme contents in liver were largely unaffected during torpor. Under these conditions, different WAT depots responded by elevating the amounts of specific enzymes, as follows: SOD 1 in retroperitoneal WAT, GSH-Px in gonadal WAT, and CAT in subcutaneous WAT. Similar perturbations of AD enzymes contents were seen in all tissues during cold acclimation, often in a time-dependent manner. It can be concluded that BAT and muscle AD capacity undergo the most dramatic changes during both cold acclimation and hibernation, while liver is relatively unaffected by either condition. Additionally, this study provides a basis for further metabolic study that will illuminate the causes of these tissue-specific AD responses, particularly the novel finding of distinct responses by different WAT depots in hibernators. (C) 2013 Elsevier Inc. All rights reserved.
T2  - Free Radical Biology and Medicine
T1  - The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update
IS  - null
VL  - 65
SP  - 47
EP  - 924
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_944
ER  - 

@article{
author = "Vučetić, Milica and Stančić, Ana and Otašević, Vesna and Janković, Aleksandra and Korac, Aleksandra B and Markelić, Milica B and Velicković, Ksenija D and Golić, Igor and Buzadžić, Biljana J. and Storey, Kenneth B and Korać, Bato",
year = "2013",
abstract = "Any alteration in oxidative metabolism is coupled with a corresponding response by an antioxidant defense (AD) in appropriate subcellular compartments. Seasonal hibernators pass through circannual metabolic adaptations that allow them to either maintain euthermy (cold acclimation) or enter winter torpor with body temperature falling to low values. The present study aimed to investigate the corresponding pattern of AD enzyme protein expressions associated with these strategies in the main tissues involved in whole animal energy homeostasis: brown and white adipose tissues (BAT and WAT, respectively), liver, and skeletal muscle. European ground squirrels (Spermophilus citellus) were exposed to low temperature (4 +/- 1 C) and then divided into two groups: (1) animals fell into torpor (hibernating group) and (2) animals stayed active and euthermic for 1, 3, 7, 12, or 21 days (cold-exposed group). We examined the effects of cold acclimation and hibernation on the tissue-dependent protein expression of four enzymes which catalyze the two-step detoxification of superoxide to water: superoxide dismutase 1 and 2 (SOD 1 and 2), catalase (CAT), and glutathione peroxidase (GSH-Px). The results showed that hibernation induced an increase of AD enzyme protein expressions in BAT and skeletal muscle. However, AD enzyme contents in liver were largely unaffected during torpor. Under these conditions, different WAT depots responded by elevating the amounts of specific enzymes, as follows: SOD 1 in retroperitoneal WAT, GSH-Px in gonadal WAT, and CAT in subcutaneous WAT. Similar perturbations of AD enzymes contents were seen in all tissues during cold acclimation, often in a time-dependent manner. It can be concluded that BAT and muscle AD capacity undergo the most dramatic changes during both cold acclimation and hibernation, while liver is relatively unaffected by either condition. Additionally, this study provides a basis for further metabolic study that will illuminate the causes of these tissue-specific AD responses, particularly the novel finding of distinct responses by different WAT depots in hibernators. (C) 2013 Elsevier Inc. All rights reserved.",
journal = "Free Radical Biology and Medicine",
title = "The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update",
number = "null",
volume = "65",
pages = "47-924",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_944"
}

Vučetić, M., Stančić, A., Otašević, V., Janković, A., Korac, A. B., Markelić, M. B., Velicković, K. D., Golić, I., Buzadžić, B. J., Storey, K. B.,& Korać, B.. (2013). The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update. in Free Radical Biology and Medicine, 65(null), 47-924.
https://hdl.handle.net/21.15107/rcub_ibiss_944

Vučetić M, Stančić A, Otašević V, Janković A, Korac AB, Markelić MB, Velicković KD, Golić I, Buzadžić BJ, Storey KB, Korać B. The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update. in Free Radical Biology and Medicine. 2013;65(null):47-924.
https://hdl.handle.net/21.15107/rcub_ibiss_944 .

Vučetić, Milica, Stančić, Ana, Otašević, Vesna, Janković, Aleksandra, Korac, Aleksandra B, Markelić, Milica B, Velicković, Ksenija D, Golić, Igor, Buzadžić, Biljana J., Storey, Kenneth B, Korać, Bato, "The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update" in Free Radical Biology and Medicine, 65, no. null (2013):47-924,
https://hdl.handle.net/21.15107/rcub_ibiss_944 .

TY  - JOUR
AU  - Vučetić, Milica
AU  - Otašević, Vesna
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Markelić, Milica B
AU  - Golić, Igor
AU  - Velicković, Ksenija D
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
AU  - Korać, Bato
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1148
AB  - In this study, the effects of l-arginine-nitric-oxide ((NO)-N-a (TM))-producing pathway on protein content of ubiquitin, as an important component of ubiquitin-proteasome system for protein removal, were investigated. We showed that l-arginine markedly decreased ubiquitin protein content in interscapular brown adipose tissue, both in thermogenic inactive (at room temperature) and thermogenic active (on cold) states; while in l-NAME-treated groups this effect was abolished. This result suggests that nitric oxide ((NO)-N-a (TM)), besides well established roles, is involved in this aspect of structure remodeling, as well.
T2  - Molecular and Cellular Biochemistry
T1  - Protein expression of ubiquitin in interscapular brown adipose tissue during acclimation of rats to cold: the impact of (NO)-N-center dot
IS  - 1-2
VL  - 368
SP  - 503
EP  - 193
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1148
ER  - 

@article{
author = "Vučetić, Milica and Otašević, Vesna and Stančić, Ana and Janković, Aleksandra and Markelić, Milica B and Golić, Igor and Velicković, Ksenija D and Buzadžić, Biljana J. and Korac, Aleksandra B and Korać, Bato",
year = "2012",
abstract = "In this study, the effects of l-arginine-nitric-oxide ((NO)-N-a (TM))-producing pathway on protein content of ubiquitin, as an important component of ubiquitin-proteasome system for protein removal, were investigated. We showed that l-arginine markedly decreased ubiquitin protein content in interscapular brown adipose tissue, both in thermogenic inactive (at room temperature) and thermogenic active (on cold) states; while in l-NAME-treated groups this effect was abolished. This result suggests that nitric oxide ((NO)-N-a (TM)), besides well established roles, is involved in this aspect of structure remodeling, as well.",
journal = "Molecular and Cellular Biochemistry",
title = "Protein expression of ubiquitin in interscapular brown adipose tissue during acclimation of rats to cold: the impact of (NO)-N-center dot",
number = "1-2",
volume = "368",
pages = "503-193",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1148"
}

Vučetić, M., Otašević, V., Stančić, A., Janković, A., Markelić, M. B., Golić, I., Velicković, K. D., Buzadžić, B. J., Korac, A. B.,& Korać, B.. (2012). Protein expression of ubiquitin in interscapular brown adipose tissue during acclimation of rats to cold: the impact of (NO)-N-center dot. in Molecular and Cellular Biochemistry, 368(1-2), 503-193.
https://hdl.handle.net/21.15107/rcub_ibiss_1148

Vučetić M, Otašević V, Stančić A, Janković A, Markelić MB, Golić I, Velicković KD, Buzadžić BJ, Korac AB, Korać B. Protein expression of ubiquitin in interscapular brown adipose tissue during acclimation of rats to cold: the impact of (NO)-N-center dot. in Molecular and Cellular Biochemistry. 2012;368(1-2):503-193.
https://hdl.handle.net/21.15107/rcub_ibiss_1148 .

Vučetić, Milica, Otašević, Vesna, Stančić, Ana, Janković, Aleksandra, Markelić, Milica B, Golić, Igor, Velicković, Ksenija D, Buzadžić, Biljana J., Korac, Aleksandra B, Korać, Bato, "Protein expression of ubiquitin in interscapular brown adipose tissue during acclimation of rats to cold: the impact of (NO)-N-center dot" in Molecular and Cellular Biochemistry, 368, no. 1-2 (2012):503-193,
https://hdl.handle.net/21.15107/rcub_ibiss_1148 .

TY  - JOUR
AU  - Jović, Miomir Đ
AU  - Stančić, Ana
AU  - Nenadić, Dragan
AU  - Cekić, Olivera
AU  - Nezić, Dusko G
AU  - Milojević, Predrag S
AU  - Micović, Slobodan V
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
AU  - Otašević, Vesna
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Korać, Bato
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1138
AB  - Background/Aims: Study elucidates and compares the mitochondrial bioenergetic-related molecular basis of sevoflurane and propofol cardioprotection during aortic valve replacement surgery due to aortic valve stenosis. Methods: Twenty-two patients were prospectively randomized in two groups regarding the anesthetic regime: sevoflurane and propofol. Hemodynamic parameters, biomarkers of cardiac injury and brain natriuretic peptide (BNP) were measured preoperatively and postoperatively. In tissue samples, taken from the interventricular septum, key mitochondrial molecules were determined by Western blot, real time PCR, as well as confocal microscopy and immunohisto- and immunocyto-chemical analysis. Results: The protein levels of cytochrome c oxidase and ATP synthase were higher in sevoflurane than in propofol group. Nevertheless, cytochrome c protein content was higher in propofol than sevoflurane receiving patients. Propofol group also showed higher protein level of connexin 43 (Cx43) than sevoflurane group. Besides, immunogold analysis showed its mitochondrial localization. The mRNA level of mtDNA and uncoupling protein (UCP2) were higher in propofol than sevoflurane patients, as well. On the other hand, there were no significant differences between groups in hemodynamic assessment, intensive care unit length of stay, troponin I and BNP level. Conclusions: Our data indicate that sevoflurane and propofol lead to cardiac protection via different mitochondrially related molecular mechanisms. It appears that sevoflurane acts regulating cytochrome c oxidase and ATP synthase, while the effects of propofol occur through regulation of cytochrome c, Cx43, mtDNA transcription and UCP2. Copyright (C) 2012 S. Karger AG, Basel
T2  - Cellular Physiology and Biochemistry
T1  - Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass
IS  - 1-2
VL  - 29
SP  - 973
EP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1138
ER  - 

@article{
author = "Jović, Miomir Đ and Stančić, Ana and Nenadić, Dragan and Cekić, Olivera and Nezić, Dusko G and Milojević, Predrag S and Micović, Slobodan V and Buzadžić, Biljana J. and Korac, Aleksandra B and Otašević, Vesna and Janković, Aleksandra and Vučetić, Milica and Velicković, Ksenija D and Golić, Igor and Korać, Bato",
year = "2012",
abstract = "Background/Aims: Study elucidates and compares the mitochondrial bioenergetic-related molecular basis of sevoflurane and propofol cardioprotection during aortic valve replacement surgery due to aortic valve stenosis. Methods: Twenty-two patients were prospectively randomized in two groups regarding the anesthetic regime: sevoflurane and propofol. Hemodynamic parameters, biomarkers of cardiac injury and brain natriuretic peptide (BNP) were measured preoperatively and postoperatively. In tissue samples, taken from the interventricular septum, key mitochondrial molecules were determined by Western blot, real time PCR, as well as confocal microscopy and immunohisto- and immunocyto-chemical analysis. Results: The protein levels of cytochrome c oxidase and ATP synthase were higher in sevoflurane than in propofol group. Nevertheless, cytochrome c protein content was higher in propofol than sevoflurane receiving patients. Propofol group also showed higher protein level of connexin 43 (Cx43) than sevoflurane group. Besides, immunogold analysis showed its mitochondrial localization. The mRNA level of mtDNA and uncoupling protein (UCP2) were higher in propofol than sevoflurane patients, as well. On the other hand, there were no significant differences between groups in hemodynamic assessment, intensive care unit length of stay, troponin I and BNP level. Conclusions: Our data indicate that sevoflurane and propofol lead to cardiac protection via different mitochondrially related molecular mechanisms. It appears that sevoflurane acts regulating cytochrome c oxidase and ATP synthase, while the effects of propofol occur through regulation of cytochrome c, Cx43, mtDNA transcription and UCP2. Copyright (C) 2012 S. Karger AG, Basel",
journal = "Cellular Physiology and Biochemistry",
title = "Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass",
number = "1-2",
volume = "29",
pages = "973-142",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1138"
}

Jović, M. Đ., Stančić, A., Nenadić, D., Cekić, O., Nezić, D. G., Milojević, P. S., Micović, S. V., Buzadžić, B. J., Korac, A. B., Otašević, V., Janković, A., Vučetić, M., Velicković, K. D., Golić, I.,& Korać, B.. (2012). Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass. in Cellular Physiology and Biochemistry, 29(1-2), 973-142.
https://hdl.handle.net/21.15107/rcub_ibiss_1138

Jović MĐ, Stančić A, Nenadić D, Cekić O, Nezić DG, Milojević PS, Micović SV, Buzadžić BJ, Korac AB, Otašević V, Janković A, Vučetić M, Velicković KD, Golić I, Korać B. Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass. in Cellular Physiology and Biochemistry. 2012;29(1-2):973-142.
https://hdl.handle.net/21.15107/rcub_ibiss_1138 .

Jović, Miomir Đ, Stančić, Ana, Nenadić, Dragan, Cekić, Olivera, Nezić, Dusko G, Milojević, Predrag S, Micović, Slobodan V, Buzadžić, Biljana J., Korac, Aleksandra B, Otašević, Vesna, Janković, Aleksandra, Vučetić, Milica, Velicković, Ksenija D, Golić, Igor, Korać, Bato, "Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass" in Cellular Physiology and Biochemistry, 29, no. 1-2 (2012):973-142,
https://hdl.handle.net/21.15107/rcub_ibiss_1138 .

TY  - CONF
AU  - Vučetić, Milica
AU  - Stančić, Ana
AU  - Filipović, Milos R
AU  - Ivanović-Burmazović, Ivana S
AU  - Otašević, Vesna
AU  - Korac, Aleksandra B
AU  - Janković, Aleksandra
AU  - Buzadžić, Biljana J.
AU  - Velicković, Ksenija D
AU  - Markelić, Milica B
AU  - Golić, Igor
AU  - Korać, Bato
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1137
C3  - European Journal of Clinical Investigation
T1  - The effects of superoxide dismutase mimic on energy metabolism in hippocampus of diabetic rats
IS  - null
VL  - 42
SP  - 969
EP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1137
ER  - 

@conference{
author = "Vučetić, Milica and Stančić, Ana and Filipović, Milos R and Ivanović-Burmazović, Ivana S and Otašević, Vesna and Korac, Aleksandra B and Janković, Aleksandra and Buzadžić, Biljana J. and Velicković, Ksenija D and Markelić, Milica B and Golić, Igor and Korać, Bato",
year = "2012",
journal = "European Journal of Clinical Investigation",
title = "The effects of superoxide dismutase mimic on energy metabolism in hippocampus of diabetic rats",
number = "null",
volume = "42",
pages = "969-76",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1137"
}

Vučetić, M., Stančić, A., Filipović, M. R., Ivanović-Burmazović, I. S., Otašević, V., Korac, A. B., Janković, A., Buzadžić, B. J., Velicković, K. D., Markelić, M. B., Golić, I.,& Korać, B.. (2012). The effects of superoxide dismutase mimic on energy metabolism in hippocampus of diabetic rats. in European Journal of Clinical Investigation, 42(null), 969-76.
https://hdl.handle.net/21.15107/rcub_ibiss_1137

Vučetić M, Stančić A, Filipović MR, Ivanović-Burmazović IS, Otašević V, Korac AB, Janković A, Buzadžić BJ, Velicković KD, Markelić MB, Golić I, Korać B. The effects of superoxide dismutase mimic on energy metabolism in hippocampus of diabetic rats. in European Journal of Clinical Investigation. 2012;42(null):969-76.
https://hdl.handle.net/21.15107/rcub_ibiss_1137 .

Vučetić, Milica, Stančić, Ana, Filipović, Milos R, Ivanović-Burmazović, Ivana S, Otašević, Vesna, Korac, Aleksandra B, Janković, Aleksandra, Buzadžić, Biljana J., Velicković, Ksenija D, Markelić, Milica B, Golić, Igor, Korać, Bato, "The effects of superoxide dismutase mimic on energy metabolism in hippocampus of diabetic rats" in European Journal of Clinical Investigation, 42, no. null (2012):969-76,
https://hdl.handle.net/21.15107/rcub_ibiss_1137 .

TY  - CONF
AU  - Macanović, Biljana
AU  - Otašević, Vesna
AU  - Korac, Aleksandra B
AU  - Vučetić, Milica
AU  - Garalejić, Eliana
AU  - Ivanović-Burmazović, Ivana S
AU  - Filipović, Milos R
AU  - Buzadžić, Biljana J.
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Markelić, Milica B
AU  - Korać, Bato
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1212
C3  - Human Reproduction
T1  - Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO
IS  - null
VL  - 27
EP  - na
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1212
ER  - 

@conference{
author = "Macanović, Biljana and Otašević, Vesna and Korac, Aleksandra B and Vučetić, Milica and Garalejić, Eliana and Ivanović-Burmazović, Ivana S and Filipović, Milos R and Buzadžić, Biljana J. and Stančić, Ana and Janković, Aleksandra and Velicković, Ksenija D and Golić, Igor and Markelić, Milica B and Korać, Bato",
year = "2012",
journal = "Human Reproduction",
title = "Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO",
number = "null",
volume = "27",
pages = "na",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1212"
}

Macanović, B., Otašević, V., Korac, A. B., Vučetić, M., Garalejić, E., Ivanović-Burmazović, I. S., Filipović, M. R., Buzadžić, B. J., Stančić, A., Janković, A., Velicković, K. D., Golić, I., Markelić, M. B.,& Korać, B.. (2012). Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO. in Human Reproduction, 27(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1212

Macanović B, Otašević V, Korac AB, Vučetić M, Garalejić E, Ivanović-Burmazović IS, Filipović MR, Buzadžić BJ, Stančić A, Janković A, Velicković KD, Golić I, Markelić MB, Korać B. Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO. in Human Reproduction. 2012;27(null):null-na.
https://hdl.handle.net/21.15107/rcub_ibiss_1212 .

Macanović, Biljana, Otašević, Vesna, Korac, Aleksandra B, Vučetić, Milica, Garalejić, Eliana, Ivanović-Burmazović, Ivana S, Filipović, Milos R, Buzadžić, Biljana J., Stančić, Ana, Janković, Aleksandra, Velicković, Ksenija D, Golić, Igor, Markelić, Milica B, Korać, Bato, "Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO" in Human Reproduction, 27, no. null (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1212 .

TY  - JOUR
AU  - Vučetić, Milica
AU  - Otašević, Vesna
AU  - Korac, Aleksandra B
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Markelić, Milica B
AU  - Golić, Igor
AU  - Velicković, Ksenija D
AU  - Buzadžić, Biljana J.
AU  - Korać, Bato
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1243
AB  - Background: Brown adipose tissue thermogenic program includes complex molecular and structural changes. However, energetic aspects of this process are poorly depicted. Methods: We investigated time-dependent reprogramming of interscapular brown adipose tissue (IBAT) energy metabolism during cold-acclimation, as well as the effects of nitric oxide ((center dot)NO) on those changes. Rats were exposed to cold (4 +/- 1 degrees C) for periods of 1, 3, 7, 12, 21. and 45 days, and divided into three groups: control, treated with L-arginine, and treated with N(omega)-nitro-L-arginine methyl ester (L-NAME). Results: In the early phase of cold-acclimation (up to 7 days), the protein levels of all metabolic parameters and oxidative phosphorylation components were below the control. However, metabolic parameters and respiratory chain components entered a new homeostatic level in the late phase of cold-acclimation. These changes were accompanied with increased protein levels of phospho-AMP-dependent protein kinase-alpha (phospho-AMPK alpha) on the first day of cold-acclimation, and hypoxia-inducible factor-1 alpha (HIF-1 alpha) throughout early cold-acclimation. L-arginine positively affected protein expression of enzymes involved in glucose metabolism and beta-oxidation of fatty acids in the early phase of cold-acclimation, and oxidative phosphorylation components throughout cold-acclimation. In contrast, L-NAME had the opposite effects. Conclusion: Results suggest that IBAT structural remodeling is followed by energy metabolism reprogramming, which control might be orchestrated by the action of AMPK alpha and HIF-1 alpha. Data also indicated the involvement of L-arginine-(center dot)NO in the regulation of IBAT metabolism. General significance: Results obtained in this study might be of great importance for elucidating regulatory pathways governing energy metabolism in both physiological and pathophysiological states. (C) 2011 Elsevier B.V. All rights reserved.
T2  - Biochimica et Biophysica Acta-General Subjects
T1  - Interscapular brown adipose tissue metabolic reprogramming during cold acclimation: Interplay of HIF-1 alpha and AMPK alpha
IS  - 12
VL  - 1810
EP  - 1261
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1243
ER  - 

@article{
author = "Vučetić, Milica and Otašević, Vesna and Korac, Aleksandra B and Stančić, Ana and Janković, Aleksandra and Markelić, Milica B and Golić, Igor and Velicković, Ksenija D and Buzadžić, Biljana J. and Korać, Bato",
year = "2011",
abstract = "Background: Brown adipose tissue thermogenic program includes complex molecular and structural changes. However, energetic aspects of this process are poorly depicted. Methods: We investigated time-dependent reprogramming of interscapular brown adipose tissue (IBAT) energy metabolism during cold-acclimation, as well as the effects of nitric oxide ((center dot)NO) on those changes. Rats were exposed to cold (4 +/- 1 degrees C) for periods of 1, 3, 7, 12, 21. and 45 days, and divided into three groups: control, treated with L-arginine, and treated with N(omega)-nitro-L-arginine methyl ester (L-NAME). Results: In the early phase of cold-acclimation (up to 7 days), the protein levels of all metabolic parameters and oxidative phosphorylation components were below the control. However, metabolic parameters and respiratory chain components entered a new homeostatic level in the late phase of cold-acclimation. These changes were accompanied with increased protein levels of phospho-AMP-dependent protein kinase-alpha (phospho-AMPK alpha) on the first day of cold-acclimation, and hypoxia-inducible factor-1 alpha (HIF-1 alpha) throughout early cold-acclimation. L-arginine positively affected protein expression of enzymes involved in glucose metabolism and beta-oxidation of fatty acids in the early phase of cold-acclimation, and oxidative phosphorylation components throughout cold-acclimation. In contrast, L-NAME had the opposite effects. Conclusion: Results suggest that IBAT structural remodeling is followed by energy metabolism reprogramming, which control might be orchestrated by the action of AMPK alpha and HIF-1 alpha. Data also indicated the involvement of L-arginine-(center dot)NO in the regulation of IBAT metabolism. General significance: Results obtained in this study might be of great importance for elucidating regulatory pathways governing energy metabolism in both physiological and pathophysiological states. (C) 2011 Elsevier B.V. All rights reserved.",
journal = "Biochimica et Biophysica Acta-General Subjects",
title = "Interscapular brown adipose tissue metabolic reprogramming during cold acclimation: Interplay of HIF-1 alpha and AMPK alpha",
number = "12",
volume = "1810",
pages = "1261",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1243"
}

Vučetić, M., Otašević, V., Korac, A. B., Stančić, A., Janković, A., Markelić, M. B., Golić, I., Velicković, K. D., Buzadžić, B. J.,& Korać, B.. (2011). Interscapular brown adipose tissue metabolic reprogramming during cold acclimation: Interplay of HIF-1 alpha and AMPK alpha. in Biochimica et Biophysica Acta-General Subjects, 1810(12).
https://hdl.handle.net/21.15107/rcub_ibiss_1243

Vučetić M, Otašević V, Korac AB, Stančić A, Janković A, Markelić MB, Golić I, Velicković KD, Buzadžić BJ, Korać B. Interscapular brown adipose tissue metabolic reprogramming during cold acclimation: Interplay of HIF-1 alpha and AMPK alpha. in Biochimica et Biophysica Acta-General Subjects. 2011;1810(12):null-1261.
https://hdl.handle.net/21.15107/rcub_ibiss_1243 .

Vučetić, Milica, Otašević, Vesna, Korac, Aleksandra B, Stančić, Ana, Janković, Aleksandra, Markelić, Milica B, Golić, Igor, Velicković, Ksenija D, Buzadžić, Biljana J., Korać, Bato, "Interscapular brown adipose tissue metabolic reprogramming during cold acclimation: Interplay of HIF-1 alpha and AMPK alpha" in Biochimica et Biophysica Acta-General Subjects, 1810, no. 12 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1243 .

TY  - JOUR
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Otašević, Vesna
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Buzadžić, Biljana J.
AU  - Korać, Bato
AU  - Korac, Aleksandra B
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1324
AB  - The aim of the present study was to investigate whether hyperinsulinaemia, which frequently precedes insulin resistance syndrome (obesity, diabetes), induces apoptosis of endothelial cells (ECs) in brown adipose tissue (BAT) and causes BAT atrophy and also, to investigate the possible mechanisms underlying ECs death. In order to induce hyperinsulinaemia, adult male rats of Wistar strain were treated with high dose of insulin (4 U/kg, intraperitonely) for one or three days. Examinations at ultrastructural level showed apoptotic changes of ECs, allowing us to point out that changes mainly but not exclusively, occur in nuclei. Besides different stages of condensation and alterations of the chromatin, nuclear fragmentation was also observed. Higher number of ECs apoptotic nuclei in the BAT of hyperinsulinaemic rats was also confirmed by propidium iodide staining. Immunohistochemical localization of tumor necrosis factor-alpha (TNF-alpha) revealed increased expression in ECs of BAT of hyperinsulinaemic animals, indicating its possible role in insulin-induced apoptotic changes. These results suggest that BAT atrophy in hyperinsulinaemia is a result of endothelial and adipocyte apoptosis combined, rather than any of functional components alone.
T2  - European Journal of Histochemistry
T1  - Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-alpha
IS  - 4
VL  - 55
EP  - 193
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1324
ER  - 

@article{
author = "Markelić, Milica B and Velicković, Ksenija D and Golić, Igor and Otašević, Vesna and Stančić, Ana and Janković, Aleksandra and Vučetić, Milica and Buzadžić, Biljana J. and Korać, Bato and Korac, Aleksandra B",
year = "2011",
abstract = "The aim of the present study was to investigate whether hyperinsulinaemia, which frequently precedes insulin resistance syndrome (obesity, diabetes), induces apoptosis of endothelial cells (ECs) in brown adipose tissue (BAT) and causes BAT atrophy and also, to investigate the possible mechanisms underlying ECs death. In order to induce hyperinsulinaemia, adult male rats of Wistar strain were treated with high dose of insulin (4 U/kg, intraperitonely) for one or three days. Examinations at ultrastructural level showed apoptotic changes of ECs, allowing us to point out that changes mainly but not exclusively, occur in nuclei. Besides different stages of condensation and alterations of the chromatin, nuclear fragmentation was also observed. Higher number of ECs apoptotic nuclei in the BAT of hyperinsulinaemic rats was also confirmed by propidium iodide staining. Immunohistochemical localization of tumor necrosis factor-alpha (TNF-alpha) revealed increased expression in ECs of BAT of hyperinsulinaemic animals, indicating its possible role in insulin-induced apoptotic changes. These results suggest that BAT atrophy in hyperinsulinaemia is a result of endothelial and adipocyte apoptosis combined, rather than any of functional components alone.",
journal = "European Journal of Histochemistry",
title = "Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-alpha",
number = "4",
volume = "55",
pages = "193",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1324"
}

Markelić, M. B., Velicković, K. D., Golić, I., Otašević, V., Stančić, A., Janković, A., Vučetić, M., Buzadžić, B. J., Korać, B.,& Korac, A. B.. (2011). Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-alpha. in European Journal of Histochemistry, 55(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1324

Markelić MB, Velicković KD, Golić I, Otašević V, Stančić A, Janković A, Vučetić M, Buzadžić BJ, Korać B, Korac AB. Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-alpha. in European Journal of Histochemistry. 2011;55(4):null-193.
https://hdl.handle.net/21.15107/rcub_ibiss_1324 .

Markelić, Milica B, Velicković, Ksenija D, Golić, Igor, Otašević, Vesna, Stančić, Ana, Janković, Aleksandra, Vučetić, Milica, Buzadžić, Biljana J., Korać, Bato, Korac, Aleksandra B, "Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-alpha" in European Journal of Histochemistry, 55, no. 4 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1324 .

TY  - CONF
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Otašević, Vesna
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Korać, Bato
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1314
C3  - Journal of Vascular Research
T1  - Insulin-induced microcirculation Remodelling in the rat brown adipose tissue
IS  - null
VL  - 48
EP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1314
ER  - 

@conference{
author = "Markelić, Milica B and Velicković, Ksenija D and Golić, Igor and Otašević, Vesna and Stančić, Ana and Janković, Aleksandra and Vučetić, Milica and Korać, Bato and Buzadžić, Biljana J. and Korac, Aleksandra B",
year = "2011",
journal = "Journal of Vascular Research",
title = "Insulin-induced microcirculation Remodelling in the rat brown adipose tissue",
number = "null",
volume = "48",
pages = "135",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1314"
}

Markelić, M. B., Velicković, K. D., Golić, I., Otašević, V., Stančić, A., Janković, A., Vučetić, M., Korać, B., Buzadžić, B. J.,& Korac, A. B.. (2011). Insulin-induced microcirculation Remodelling in the rat brown adipose tissue. in Journal of Vascular Research, 48(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1314

Markelić MB, Velicković KD, Golić I, Otašević V, Stančić A, Janković A, Vučetić M, Korać B, Buzadžić BJ, Korac AB. Insulin-induced microcirculation Remodelling in the rat brown adipose tissue. in Journal of Vascular Research. 2011;48(null):null-135.
https://hdl.handle.net/21.15107/rcub_ibiss_1314 .

Markelić, Milica B, Velicković, Ksenija D, Golić, Igor, Otašević, Vesna, Stančić, Ana, Janković, Aleksandra, Vučetić, Milica, Korać, Bato, Buzadžić, Biljana J., Korac, Aleksandra B, "Insulin-induced microcirculation Remodelling in the rat brown adipose tissue" in Journal of Vascular Research, 48, no. null (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1314 .