TY  - JOUR
AU  - Savić, Jelena
AU  - Mačukanović-Jocić, Marina
AU  - Jarić, Snežana
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S1876382018307522?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3288
AB  - INTRODUCTION The study provides significant ethnobotanical information on plant resources traditionally used in the Javor Mountain region (eastern Bosnia and Herzegovina), aiming at identifying medicinal plants and collecting information concerning the specific methods of their therapeutic application. METHODS A qualitative anthropological method using a semi-structured questionnaire was used to interview 70 people. The relative importance of wild plant species, as reported by the informants, was assessed by the quantitative methods: use-value (UV), cultural importance value (CIV) and Shannon-Wiener index. Also, the level of homogeneity among information provided by different informants was calculated by the Informants’ Consensus Factor (FIC). RESULTS 73 plant species with medicinal properties were collected, identified and their methods of preparation and uses were recorded. Asteraceae, Lamiaceae and Rosaceae have the greatest species diversity. The most commonly used are Hypericum perforatum, Mentha x piperita, Sambucus nigra and Аchillea millefolium, with UV over 0.9. The most frequently used plant parts are the aerial part (29%) and the leaf (26%), while infusion being the most common way of preparing herbal remedies (57%). The plant most appreciated by the people interviewed was Hypericum perforatum used to treat skin complaints, haemorrhoids, moderate depression, gastrointestinal ailments and respiratory infections. Also, phytotherapeutic use of Veronica officinalis rhizome in jaundice treatment, as well as utilization of Prunus domestica bark for cavity protection were noted. CONCLUSION The results of the current study may be significant in rural development programs in the Javor Mountain region, in aiming to foster community-based strategies of management of natural resources.
T2  - European Journal of Integrative Medicine
T1  - Medical ethnobotany on the Javor Mountain (Bosnia and Herzegovina)
VL  - 27
DO  - 10.1016/J.EUJIM.2019.02.007
SP  - 52
EP  - 64
ER  - 

@article{
author = "Savić, Jelena and Mačukanović-Jocić, Marina and Jarić, Snežana",
year = "2019",
abstract = "INTRODUCTION The study provides significant ethnobotanical information on plant resources traditionally used in the Javor Mountain region (eastern Bosnia and Herzegovina), aiming at identifying medicinal plants and collecting information concerning the specific methods of their therapeutic application. METHODS A qualitative anthropological method using a semi-structured questionnaire was used to interview 70 people. The relative importance of wild plant species, as reported by the informants, was assessed by the quantitative methods: use-value (UV), cultural importance value (CIV) and Shannon-Wiener index. Also, the level of homogeneity among information provided by different informants was calculated by the Informants’ Consensus Factor (FIC). RESULTS 73 plant species with medicinal properties were collected, identified and their methods of preparation and uses were recorded. Asteraceae, Lamiaceae and Rosaceae have the greatest species diversity. The most commonly used are Hypericum perforatum, Mentha x piperita, Sambucus nigra and Аchillea millefolium, with UV over 0.9. The most frequently used plant parts are the aerial part (29%) and the leaf (26%), while infusion being the most common way of preparing herbal remedies (57%). The plant most appreciated by the people interviewed was Hypericum perforatum used to treat skin complaints, haemorrhoids, moderate depression, gastrointestinal ailments and respiratory infections. Also, phytotherapeutic use of Veronica officinalis rhizome in jaundice treatment, as well as utilization of Prunus domestica bark for cavity protection were noted. CONCLUSION The results of the current study may be significant in rural development programs in the Javor Mountain region, in aiming to foster community-based strategies of management of natural resources.",
journal = "European Journal of Integrative Medicine",
title = "Medical ethnobotany on the Javor Mountain (Bosnia and Herzegovina)",
volume = "27",
doi = "10.1016/J.EUJIM.2019.02.007",
pages = "52-64"
}

Savić, J., Mačukanović-Jocić, M.,& Jarić, S.. (2019). Medical ethnobotany on the Javor Mountain (Bosnia and Herzegovina). in European Journal of Integrative Medicine, 27, 52-64.
https://doi.org/10.1016/J.EUJIM.2019.02.007

Savić J, Mačukanović-Jocić M, Jarić S. Medical ethnobotany on the Javor Mountain (Bosnia and Herzegovina). in European Journal of Integrative Medicine. 2019;27:52-64.
doi:10.1016/J.EUJIM.2019.02.007 .

Savić, Jelena, Mačukanović-Jocić, Marina, Jarić, Snežana, "Medical ethnobotany on the Javor Mountain (Bosnia and Herzegovina)" in European Journal of Integrative Medicine, 27 (2019):52-64,
https://doi.org/10.1016/J.EUJIM.2019.02.007 . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan V
AU  - Banjac, Nevena
AU  - Cingel, Aleksandar
AU  - Savić, Jelena M.
AU  - Bohanec, Borut
AU  - Ninković, Slavica
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1007
AB  - A simple and efficient protocol for direct somatic embryogenesis and plant regeneration of kohlrabi (Brassica oleracea var. gongylodes) was developed. Somatic embryos were induced from immature zygotic embryos at different developmental stages cultured on Murashige and Skoog medium supplemented with 0, 0.5, 1.0, or 1.5 mg/l 2,4-dichlorophenoxyacetic acid. Zygotic embryos at the early cotyledonary stage, which were cultured for 4 wk on plant growth regulator-free (PGR-free) medium, displayed the highest percentage of somatic embryogenesis (80.7%). Embryogenic tissue could be subcultured on the same medium for over 1 yr. Embryogenic lines derived from early cotyledonary stage zygotic embryos displayed the highest intensity of secondary embryogenesis (highest mean number of new somatic embryos per responsive somatic embryo explant). Histological analyses confirmed the direct origin of the secondary somatic embryos. Prolonged culturing of embryogenic tissue on PGR-free medium led to somatic embryo development into plantlets that were successfully acclimated in the greenhouse with a survival rate of 72.5%. Flow cytometry analysis showed no ploidy variation in 96.7% of the acclimated plants.
T2  - In Vitro Cellular & Developmental Biology-Plant
T1  - In vitro plant regeneration from immature zygotic embryos and repetitive somatic embryogenesis in kohlrabi (Brassica oleracea var. gongylodes)
IS  - 3
VL  - 49
SP  - 294
EP  - 303
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1007
ER  - 

@article{
author = "Ćosić, Tatjana and Vinterhalter, Branka and Vinterhalter, Dragan V and Banjac, Nevena and Cingel, Aleksandar and Savić, Jelena M. and Bohanec, Borut and Ninković, Slavica",
year = "2013",
abstract = "A simple and efficient protocol for direct somatic embryogenesis and plant regeneration of kohlrabi (Brassica oleracea var. gongylodes) was developed. Somatic embryos were induced from immature zygotic embryos at different developmental stages cultured on Murashige and Skoog medium supplemented with 0, 0.5, 1.0, or 1.5 mg/l 2,4-dichlorophenoxyacetic acid. Zygotic embryos at the early cotyledonary stage, which were cultured for 4 wk on plant growth regulator-free (PGR-free) medium, displayed the highest percentage of somatic embryogenesis (80.7%). Embryogenic tissue could be subcultured on the same medium for over 1 yr. Embryogenic lines derived from early cotyledonary stage zygotic embryos displayed the highest intensity of secondary embryogenesis (highest mean number of new somatic embryos per responsive somatic embryo explant). Histological analyses confirmed the direct origin of the secondary somatic embryos. Prolonged culturing of embryogenic tissue on PGR-free medium led to somatic embryo development into plantlets that were successfully acclimated in the greenhouse with a survival rate of 72.5%. Flow cytometry analysis showed no ploidy variation in 96.7% of the acclimated plants.",
journal = "In Vitro Cellular & Developmental Biology-Plant",
title = "In vitro plant regeneration from immature zygotic embryos and repetitive somatic embryogenesis in kohlrabi (Brassica oleracea var. gongylodes)",
number = "3",
volume = "49",
pages = "294-303",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1007"
}

Ćosić, T., Vinterhalter, B., Vinterhalter, D. V., Banjac, N., Cingel, A., Savić, J. M., Bohanec, B.,& Ninković, S.. (2013). In vitro plant regeneration from immature zygotic embryos and repetitive somatic embryogenesis in kohlrabi (Brassica oleracea var. gongylodes). in In Vitro Cellular & Developmental Biology-Plant, 49(3), 294-303.
https://hdl.handle.net/21.15107/rcub_ibiss_1007

Ćosić T, Vinterhalter B, Vinterhalter DV, Banjac N, Cingel A, Savić JM, Bohanec B, Ninković S. In vitro plant regeneration from immature zygotic embryos and repetitive somatic embryogenesis in kohlrabi (Brassica oleracea var. gongylodes). in In Vitro Cellular & Developmental Biology-Plant. 2013;49(3):294-303.
https://hdl.handle.net/21.15107/rcub_ibiss_1007 .

Ćosić, Tatjana, Vinterhalter, Branka, Vinterhalter, Dragan V, Banjac, Nevena, Cingel, Aleksandar, Savić, Jelena M., Bohanec, Borut, Ninković, Slavica, "In vitro plant regeneration from immature zygotic embryos and repetitive somatic embryogenesis in kohlrabi (Brassica oleracea var. gongylodes)" in In Vitro Cellular & Developmental Biology-Plant, 49, no. 3 (2013):294-303,
https://hdl.handle.net/21.15107/rcub_ibiss_1007 .

TY  - JOUR
AU  - Savić, Jelena M.
AU  - Smigocki, Ann
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1087
AB  - Expression pattern of a sugar beet serine proteinase inhibitor gene, BvSTI, was characterized in response to mechanical and fall armyworm (FAW; Spodoptera frugiperda J.E. Smith) induced wounding. BvSTI expression was analyzed in three breeding lines moderately resistant to sugar beet root maggot (Tetanops myopaeformis Roder) and in a susceptible line, F1010. Increased mechanical wound induced levels of BvSTI expression were observed in all resistant lines as compared to F1010 during the first 24 h. The most intensive response to wounding was observed in one of the resistant lines, F1016, with a maximum 5- and 2.5-fold increase of BvSTI transcript levels over non-wounded roots and leaves, respectively. In contrast, slight increase of BvSTI transcript levels in leaves and even an initial decrease in roots were observed in F1010. BvSTI transcript accumulation in F1016 and F1010 tissues wounded by FAW showed a similar gene expression pattern, but it was delayed and less intense than the response incited by abiotic wounding. On the protein level, BvSTI specific antibody confirmed increased accumulation of the 30 kDa BvSTI protein in wounded leaves but not in roots of F1016 and F1010. Using trypsin inhibition assays, the activity of BvSTI was confirmed in F1016 roots and leaves and F1010 leaves. In F1010 roots BvSTI activity was completely lacking. We conclude that BvSTI gene expression was wound induced in the insect resistant germplasm suggesting that BvSTI can be used in biotechnological approaches or in breeding programs for improving insect resistance.
T2  - Euphytica
T1  - Beta vulgaris L. serine proteinase inhibitor gene expression in insect resistant sugar beet
IS  - 2
VL  - 188
SP  - 34
EP  - 198
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1087
ER  - 

@article{
author = "Savić, Jelena M. and Smigocki, Ann",
year = "2012",
abstract = "Expression pattern of a sugar beet serine proteinase inhibitor gene, BvSTI, was characterized in response to mechanical and fall armyworm (FAW; Spodoptera frugiperda J.E. Smith) induced wounding. BvSTI expression was analyzed in three breeding lines moderately resistant to sugar beet root maggot (Tetanops myopaeformis Roder) and in a susceptible line, F1010. Increased mechanical wound induced levels of BvSTI expression were observed in all resistant lines as compared to F1010 during the first 24 h. The most intensive response to wounding was observed in one of the resistant lines, F1016, with a maximum 5- and 2.5-fold increase of BvSTI transcript levels over non-wounded roots and leaves, respectively. In contrast, slight increase of BvSTI transcript levels in leaves and even an initial decrease in roots were observed in F1010. BvSTI transcript accumulation in F1016 and F1010 tissues wounded by FAW showed a similar gene expression pattern, but it was delayed and less intense than the response incited by abiotic wounding. On the protein level, BvSTI specific antibody confirmed increased accumulation of the 30 kDa BvSTI protein in wounded leaves but not in roots of F1016 and F1010. Using trypsin inhibition assays, the activity of BvSTI was confirmed in F1016 roots and leaves and F1010 leaves. In F1010 roots BvSTI activity was completely lacking. We conclude that BvSTI gene expression was wound induced in the insect resistant germplasm suggesting that BvSTI can be used in biotechnological approaches or in breeding programs for improving insect resistance.",
journal = "Euphytica",
title = "Beta vulgaris L. serine proteinase inhibitor gene expression in insect resistant sugar beet",
number = "2",
volume = "188",
pages = "34-198",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1087"
}

Savić, J. M.,& Smigocki, A.. (2012). Beta vulgaris L. serine proteinase inhibitor gene expression in insect resistant sugar beet. in Euphytica, 188(2), 34-198.
https://hdl.handle.net/21.15107/rcub_ibiss_1087

Savić JM, Smigocki A. Beta vulgaris L. serine proteinase inhibitor gene expression in insect resistant sugar beet. in Euphytica. 2012;188(2):34-198.
https://hdl.handle.net/21.15107/rcub_ibiss_1087 .

Savić, Jelena M., Smigocki, Ann, "Beta vulgaris L. serine proteinase inhibitor gene expression in insect resistant sugar beet" in Euphytica, 188, no. 2 (2012):34-198,
https://hdl.handle.net/21.15107/rcub_ibiss_1087 .

TY  - JOUR
AU  - Stanisavljević, Nemanja S
AU  - Savić, Jelena M.
AU  - Jovanović, Zivko S
AU  - Miljuš-Đukić, Jovanka D.
AU  - Radović, Svetlana R
AU  - Vinterhalter, Dragan V
AU  - Vinterhalter, Branka
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1142
AB  - Shoot cultures of rare Balkan hyperaccumulating species Alyssum markgrafii were subjected to high nickel concentrations of 1, 3, 5, and 8 mM. The effects of graded nickel concentrations on toxicity, pigments, and several components of plant antioxidative defense system were characterized. Toxic effects of excess nickel were observed through slower growth and biomass decrease, together with increased reactive oxygen species (ROS) production and lipid peroxidation. Nickel exposure decreased chlorophyll a, b, total chlorophyll as well as carotenoid concentration. Addition of sodium benzoate, potent ROS scavenger, showed concentration-dependent disturbing effect on nickel hyperaccumulation, lowering the content of accumulated nickel in A. markgrafii shoots. General reducing power represented by low molecular weight antioxidants and phenols was inversely correlated with nickel concentration. Among the investigated antioxidative enzymes, POD seems to play important role in ROS level regulation upon excessive nickel in medium.
T2  - Acta Physiologiae Plantarum
T1  - Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level
IS  - 5
VL  - 34
SP  - 339
EP  - 2006
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1142
ER  - 

@article{
author = "Stanisavljević, Nemanja S and Savić, Jelena M. and Jovanović, Zivko S and Miljuš-Đukić, Jovanka D. and Radović, Svetlana R and Vinterhalter, Dragan V and Vinterhalter, Branka",
year = "2012",
abstract = "Shoot cultures of rare Balkan hyperaccumulating species Alyssum markgrafii were subjected to high nickel concentrations of 1, 3, 5, and 8 mM. The effects of graded nickel concentrations on toxicity, pigments, and several components of plant antioxidative defense system were characterized. Toxic effects of excess nickel were observed through slower growth and biomass decrease, together with increased reactive oxygen species (ROS) production and lipid peroxidation. Nickel exposure decreased chlorophyll a, b, total chlorophyll as well as carotenoid concentration. Addition of sodium benzoate, potent ROS scavenger, showed concentration-dependent disturbing effect on nickel hyperaccumulation, lowering the content of accumulated nickel in A. markgrafii shoots. General reducing power represented by low molecular weight antioxidants and phenols was inversely correlated with nickel concentration. Among the investigated antioxidative enzymes, POD seems to play important role in ROS level regulation upon excessive nickel in medium.",
journal = "Acta Physiologiae Plantarum",
title = "Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level",
number = "5",
volume = "34",
pages = "339-2006",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1142"
}

Stanisavljević, N. S., Savić, J. M., Jovanović, Z. S., Miljuš-Đukić, J. D., Radović, S. R., Vinterhalter, D. V.,& Vinterhalter, B.. (2012). Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level. in Acta Physiologiae Plantarum, 34(5), 339-2006.
https://hdl.handle.net/21.15107/rcub_ibiss_1142

Stanisavljević NS, Savić JM, Jovanović ZS, Miljuš-Đukić JD, Radović SR, Vinterhalter DV, Vinterhalter B. Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level. in Acta Physiologiae Plantarum. 2012;34(5):339-2006.
https://hdl.handle.net/21.15107/rcub_ibiss_1142 .

Stanisavljević, Nemanja S, Savić, Jelena M., Jovanović, Zivko S, Miljuš-Đukić, Jovanka D., Radović, Svetlana R, Vinterhalter, Dragan V, Vinterhalter, Branka, "Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level" in Acta Physiologiae Plantarum, 34, no. 5 (2012):339-2006,
https://hdl.handle.net/21.15107/rcub_ibiss_1142 .

TY  - CONF
AU  - Ninković, Slavica
AU  - Smigocki, Ann
AU  - Nikolić, Radomirka R
AU  - Banjac, Nevena
AU  - Cingel, Aleksandar
AU  - Savić, Jelena M.
AU  - Đorđević, Tatjana
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1399
C3  - In Vitro Cellular & Developmental Biology-Animal
T1  - Genetic Engineering of Lotus corniculatus L Forage Legume with Proteinase Inhibitor Genes
IS  - null
VL  - 46
EP  - S191
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1399
ER  - 

@conference{
author = "Ninković, Slavica and Smigocki, Ann and Nikolić, Radomirka R and Banjac, Nevena and Cingel, Aleksandar and Savić, Jelena M. and Đorđević, Tatjana",
year = "2010",
journal = "In Vitro Cellular & Developmental Biology-Animal",
title = "Genetic Engineering of Lotus corniculatus L Forage Legume with Proteinase Inhibitor Genes",
number = "null",
volume = "46",
pages = "S191",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1399"
}

Ninković, S., Smigocki, A., Nikolić, R. R., Banjac, N., Cingel, A., Savić, J. M.,& Đorđević, T.. (2010). Genetic Engineering of Lotus corniculatus L Forage Legume with Proteinase Inhibitor Genes. in In Vitro Cellular & Developmental Biology-Animal, 46(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1399

Ninković S, Smigocki A, Nikolić RR, Banjac N, Cingel A, Savić JM, Đorđević T. Genetic Engineering of Lotus corniculatus L Forage Legume with Proteinase Inhibitor Genes. in In Vitro Cellular & Developmental Biology-Animal. 2010;46(null):null-S191.
https://hdl.handle.net/21.15107/rcub_ibiss_1399 .

Ninković, Slavica, Smigocki, Ann, Nikolić, Radomirka R, Banjac, Nevena, Cingel, Aleksandar, Savić, Jelena M., Đorđević, Tatjana, "Genetic Engineering of Lotus corniculatus L Forage Legume with Proteinase Inhibitor Genes" in In Vitro Cellular & Developmental Biology-Animal, 46, no. null (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1399 .

TY  - JOUR
AU  - Ninković, Slavica
AU  - Ćosić, Tatjana
AU  - Vinterhalter, Branka
AU  - Uzelac, Branka
AU  - Cingel, Aleksandar
AU  - Savić, Jelena M.
AU  - Radović, Svetlana R
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1340
AB  - Agrobacterium rhizogenes A4M70GUS-mediated transformation of two local breeding lines of sugar beet was obtained using 4-week-old seedlings. Root formation efficiency was 61.54% for SBa genotype and 36.36% for SBb genotype. Five highly proliferated hairy root lines have been established in liquid hormone-free MS medium. Transgenic nature of the hairy root clones was evaluated by GUS assay, PCR and RT-PCR analyses. Hairy root-derived calli were induced using different plant growth regulators (PGRs): auxin, auxin/cytokinin and cytokinin. The best callus induction response was achieved on MS medium containing both 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg/l thidiazuron (TDZ). Globular embryo-like structures were observed in friable callus after its prolonged cultivation on MS medium supplemented with TDZ and giberellic acid (GA(3)) at 1 mg/l each, followed by growth on MS medium containing 1% glucose and 0.5 mg/l 2,3,5-triiodobenzoic acid (TIBA). Histological analysis revealed somatic embryos at different stages of development in hairy root-derived callus of sugar beet.
T2  - Plant Cell Tissue and Organ Culture
T1  - Embryogenic responses of Beta vulgaris L. callus induced from transgenic hairy roots
IS  - 1
VL  - 103
EP  - 91
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1340
ER  - 

@article{
author = "Ninković, Slavica and Ćosić, Tatjana and Vinterhalter, Branka and Uzelac, Branka and Cingel, Aleksandar and Savić, Jelena M. and Radović, Svetlana R",
year = "2010",
abstract = "Agrobacterium rhizogenes A4M70GUS-mediated transformation of two local breeding lines of sugar beet was obtained using 4-week-old seedlings. Root formation efficiency was 61.54% for SBa genotype and 36.36% for SBb genotype. Five highly proliferated hairy root lines have been established in liquid hormone-free MS medium. Transgenic nature of the hairy root clones was evaluated by GUS assay, PCR and RT-PCR analyses. Hairy root-derived calli were induced using different plant growth regulators (PGRs): auxin, auxin/cytokinin and cytokinin. The best callus induction response was achieved on MS medium containing both 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg/l thidiazuron (TDZ). Globular embryo-like structures were observed in friable callus after its prolonged cultivation on MS medium supplemented with TDZ and giberellic acid (GA(3)) at 1 mg/l each, followed by growth on MS medium containing 1% glucose and 0.5 mg/l 2,3,5-triiodobenzoic acid (TIBA). Histological analysis revealed somatic embryos at different stages of development in hairy root-derived callus of sugar beet.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Embryogenic responses of Beta vulgaris L. callus induced from transgenic hairy roots",
number = "1",
volume = "103",
pages = "91",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1340"
}

Ninković, S., Ćosić, T., Vinterhalter, B., Uzelac, B., Cingel, A., Savić, J. M.,& Radović, S. R.. (2010). Embryogenic responses of Beta vulgaris L. callus induced from transgenic hairy roots. in Plant Cell Tissue and Organ Culture, 103(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1340

Ninković S, Ćosić T, Vinterhalter B, Uzelac B, Cingel A, Savić JM, Radović SR. Embryogenic responses of Beta vulgaris L. callus induced from transgenic hairy roots. in Plant Cell Tissue and Organ Culture. 2010;103(1):null-91.
https://hdl.handle.net/21.15107/rcub_ibiss_1340 .

Ninković, Slavica, Ćosić, Tatjana, Vinterhalter, Branka, Uzelac, Branka, Cingel, Aleksandar, Savić, Jelena M., Radović, Svetlana R, "Embryogenic responses of Beta vulgaris L. callus induced from transgenic hairy roots" in Plant Cell Tissue and Organ Culture, 103, no. 1 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1340 .

TY  - CONF
AU  - Vinterhalter, Branka S.
AU  - Savić, Jelena M.
AU  - Platiša, Jelena
AU  - Raspor, Martin
AU  - Ninković, Slavica B.
AU  - Banjac, Nevena
AU  - Vinterhalter, Dragan V
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1518
AB  - Shoot cultures of nickel hyperaccumulating Alyssum murale were established from epicotyl explants of seedlings aseptically germinated on hormone-free MS medium. They were further maintained on media with 0-0.92 mu M kinetin. Optimal shoot multiplication was at 0.46 mu M kinetin. Inoculation by shoot wounding was performed with overnight suspension of A. rhizogenes A4M70GUS which contains GUS gene cointegrated in pRiA4. After 30 days hairy roots were produced at the wounding site in 31 explant (25% out of 124). Hairy roots were excised and further propagated on hormone-free medium as separate clones. In the first passage clones 3 and 6 could be distinguished by fast growth and spontaneous shoot regeneration. In other clones (12, 23 and 25) shoot regeneration required presence of cytokinins. The five shoot culture clones regenerated from hairy roots were further cultured on media with 0.46 mu M kinetin. These shoots were characterized by good elongation and lateral shoot branching, short internodes, minute slightly curled leaves and well developed plagiotropic root system spreading over the surface of media. Thus all plants regenerated from hairy root cultures manifested the characteristic Ri syndrome phenotype. They all had a strong positive GUS reaction. PCR analysis confirmed presence of uidA sequence from the gus construct. They were also tolerant to nickel accumulating up to 24,700 mu g g(-1) dry weight.
C3  - Plant Cell Tissue and Organ Culture
T1  - Nickel tolerance and hyperaccumulation in shoot cultures regenerated from hairy root cultures of Alyssum murale Waldst et Kit
IS  - 3
VL  - 94
SP  - 299
EP  - 303
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1518
ER  - 

@conference{
author = "Vinterhalter, Branka S. and Savić, Jelena M. and Platiša, Jelena and Raspor, Martin and Ninković, Slavica B. and Banjac, Nevena and Vinterhalter, Dragan V",
year = "2008",
abstract = "Shoot cultures of nickel hyperaccumulating Alyssum murale were established from epicotyl explants of seedlings aseptically germinated on hormone-free MS medium. They were further maintained on media with 0-0.92 mu M kinetin. Optimal shoot multiplication was at 0.46 mu M kinetin. Inoculation by shoot wounding was performed with overnight suspension of A. rhizogenes A4M70GUS which contains GUS gene cointegrated in pRiA4. After 30 days hairy roots were produced at the wounding site in 31 explant (25% out of 124). Hairy roots were excised and further propagated on hormone-free medium as separate clones. In the first passage clones 3 and 6 could be distinguished by fast growth and spontaneous shoot regeneration. In other clones (12, 23 and 25) shoot regeneration required presence of cytokinins. The five shoot culture clones regenerated from hairy roots were further cultured on media with 0.46 mu M kinetin. These shoots were characterized by good elongation and lateral shoot branching, short internodes, minute slightly curled leaves and well developed plagiotropic root system spreading over the surface of media. Thus all plants regenerated from hairy root cultures manifested the characteristic Ri syndrome phenotype. They all had a strong positive GUS reaction. PCR analysis confirmed presence of uidA sequence from the gus construct. They were also tolerant to nickel accumulating up to 24,700 mu g g(-1) dry weight.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Nickel tolerance and hyperaccumulation in shoot cultures regenerated from hairy root cultures of Alyssum murale Waldst et Kit",
number = "3",
volume = "94",
pages = "299-303",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1518"
}

Vinterhalter, B. S., Savić, J. M., Platiša, J., Raspor, M., Ninković, S. B., Banjac, N.,& Vinterhalter, D. V.. (2008). Nickel tolerance and hyperaccumulation in shoot cultures regenerated from hairy root cultures of Alyssum murale Waldst et Kit. in Plant Cell Tissue and Organ Culture, 94(3), 299-303.
https://hdl.handle.net/21.15107/rcub_ibiss_1518

Vinterhalter BS, Savić JM, Platiša J, Raspor M, Ninković SB, Banjac N, Vinterhalter DV. Nickel tolerance and hyperaccumulation in shoot cultures regenerated from hairy root cultures of Alyssum murale Waldst et Kit. in Plant Cell Tissue and Organ Culture. 2008;94(3):299-303.
https://hdl.handle.net/21.15107/rcub_ibiss_1518 .

Vinterhalter, Branka S., Savić, Jelena M., Platiša, Jelena, Raspor, Martin, Ninković, Slavica B., Banjac, Nevena, Vinterhalter, Dragan V, "Nickel tolerance and hyperaccumulation in shoot cultures regenerated from hairy root cultures of Alyssum murale Waldst et Kit" in Plant Cell Tissue and Organ Culture, 94, no. 3 (2008):299-303,
https://hdl.handle.net/21.15107/rcub_ibiss_1518 .