TY  - JOUR
AU  - Vukšinović, Elvira L.
AU  - Pond, David W.
AU  - Grubor-Lajšić, Gordana
AU  - Worland, M. Roger
AU  - Kojić, Danijela
AU  - Purać, Jelena
AU  - Popović, Željko D.
AU  - Blagojević, Duško
PY  - 2018
UR  - http://link.springer.com/10.1007/s00360-017-1110-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2777
AB  - Larvae of the European corn borer (Ostrinia nubilalis Hubn.) were cold acclimated during different phases of diapause to determine if changes in the fatty acid composition lipids occur as part of a programmed diapause strategy, or as a response to low temperatures during winter. Cold acclimation of fifth instar larvae of O. nubilalis during diapause had modest effects further on the readjustments in fatty acid composition of triacylglycerols and phospholipids. Overall, FA unsaturation (UFAs/SFAs ratio) was stable, with the exception of the triacylglycerols fraction after exposure to −3 and −10 °C in mid-diapause (MD) when it significantly increased. Differential scanning calorimetry (DSC) was used to examine phase transitions of total body lipid of cold-acclimated larvae in diapause. Thermal analysis indicated that changes in the melt transition temperatures of whole body total lipids were subtle, but consistent with the modest changes in the level of FA unsaturation observed. We conclude that lipid rearrangements are a function of the endogenous “diapause program” rather than a direct effect of low temperatures, which proved to have limited impact on lipid changes in diapausing larvae of O. nubilalis.
T2  - Journal of Comparative Physiology B
T1  - Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls
VL  - 188
DO  - 10.1007/s00360-017-1110-9
SP  - 27
EP  - 36
ER  - 

@article{
author = "Vukšinović, Elvira L. and Pond, David W. and Grubor-Lajšić, Gordana and Worland, M. Roger and Kojić, Danijela and Purać, Jelena and Popović, Željko D. and Blagojević, Duško",
year = "2018",
abstract = "Larvae of the European corn borer (Ostrinia nubilalis Hubn.) were cold acclimated during different phases of diapause to determine if changes in the fatty acid composition lipids occur as part of a programmed diapause strategy, or as a response to low temperatures during winter. Cold acclimation of fifth instar larvae of O. nubilalis during diapause had modest effects further on the readjustments in fatty acid composition of triacylglycerols and phospholipids. Overall, FA unsaturation (UFAs/SFAs ratio) was stable, with the exception of the triacylglycerols fraction after exposure to −3 and −10 °C in mid-diapause (MD) when it significantly increased. Differential scanning calorimetry (DSC) was used to examine phase transitions of total body lipid of cold-acclimated larvae in diapause. Thermal analysis indicated that changes in the melt transition temperatures of whole body total lipids were subtle, but consistent with the modest changes in the level of FA unsaturation observed. We conclude that lipid rearrangements are a function of the endogenous “diapause program” rather than a direct effect of low temperatures, which proved to have limited impact on lipid changes in diapausing larvae of O. nubilalis.",
journal = "Journal of Comparative Physiology B",
title = "Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls",
volume = "188",
doi = "10.1007/s00360-017-1110-9",
pages = "27-36"
}

Vukšinović, E. L., Pond, D. W., Grubor-Lajšić, G., Worland, M. R., Kojić, D., Purać, J., Popović, Ž. D.,& Blagojević, D.. (2018). Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls. in Journal of Comparative Physiology B, 188, 27-36.
https://doi.org/10.1007/s00360-017-1110-9

Vukšinović EL, Pond DW, Grubor-Lajšić G, Worland MR, Kojić D, Purać J, Popović ŽD, Blagojević D. Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls. in Journal of Comparative Physiology B. 2018;188:27-36.
doi:10.1007/s00360-017-1110-9 .

Vukšinović, Elvira L., Pond, David W., Grubor-Lajšić, Gordana, Worland, M. Roger, Kojić, Danijela, Purać, Jelena, Popović, Željko D., Blagojević, Duško, "Temperature adaptation of lipids in diapausing Ostrinia nubilalis: an experimental study to distinguish environmental versus endogenous controls" in Journal of Comparative Physiology B, 188 (2018):27-36,
https://doi.org/10.1007/s00360-017-1110-9 . .

TY  - JOUR
AU  - Vukašinović, Elvira
AU  - Pond, David
AU  - Worland, Michael Roger
AU  - Kojić, Danijela
AU  - Purać, Jelena
AU  - Blagojević, Duško
AU  - Grubor-Lajšić, Gordana
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6337
AB  - This study compares the composition and biophysical properties of lipids in non-diapausing and diapausing
fifth instar larvae of Ostrinia nubilalis Hubn. (Lepidoptera: Crambidae). The majority of fat body lipids in both of these physiological states were comprised of ~90% triacylglycerols (TAGs), whereas the haemolymph contained a more even distribution of all lipid classes. The fatty acid composition and biophysical properties of the fat body lipids differed markedly between non-diapausing and diapausing larvae. Diapause was associated with a dramatic increase in the proportions of palmitoleic acid (16:1n-7) and oleic acid (18:1n-9), with concurrent reductions in palmitic acid (16:0) and linoleic acid (18:2n-6). The increase in the level of unsaturation of the fat body lipids, which caused a marked shift in their phase transitions to lower temperatures, was triggered by diapause rather than low temperatures. Adjustments of fatty acid compositions are likely to be an important component of winter diapause mechanisms, possibly maintaining the fluidity of cell membranes and the functionality of the organism during lower winter temperatures.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
T1  - Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae)
IS  - 4
VL  - 165
DO  - 10.1016/j.cbpb.2013.05.001
SP  - 219
EP  - 225
ER  - 

@article{
author = "Vukašinović, Elvira and Pond, David and Worland, Michael Roger and Kojić, Danijela and Purać, Jelena and Blagojević, Duško and Grubor-Lajšić, Gordana",
year = "2013",
abstract = "This study compares the composition and biophysical properties of lipids in non-diapausing and diapausing
fifth instar larvae of Ostrinia nubilalis Hubn. (Lepidoptera: Crambidae). The majority of fat body lipids in both of these physiological states were comprised of ~90% triacylglycerols (TAGs), whereas the haemolymph contained a more even distribution of all lipid classes. The fatty acid composition and biophysical properties of the fat body lipids differed markedly between non-diapausing and diapausing larvae. Diapause was associated with a dramatic increase in the proportions of palmitoleic acid (16:1n-7) and oleic acid (18:1n-9), with concurrent reductions in palmitic acid (16:0) and linoleic acid (18:2n-6). The increase in the level of unsaturation of the fat body lipids, which caused a marked shift in their phase transitions to lower temperatures, was triggered by diapause rather than low temperatures. Adjustments of fatty acid compositions are likely to be an important component of winter diapause mechanisms, possibly maintaining the fluidity of cell membranes and the functionality of the organism during lower winter temperatures.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology",
title = "Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae)",
number = "4",
volume = "165",
doi = "10.1016/j.cbpb.2013.05.001",
pages = "219-225"
}

Vukašinović, E., Pond, D., Worland, M. R., Kojić, D., Purać, J., Blagojević, D.,& Grubor-Lajšić, G.. (2013). Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae). in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
Elsevier Inc.., 165(4), 219-225.
https://doi.org/10.1016/j.cbpb.2013.05.001

Vukašinović E, Pond D, Worland MR, Kojić D, Purać J, Blagojević D, Grubor-Lajšić G. Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae). in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 2013;165(4):219-225.
doi:10.1016/j.cbpb.2013.05.001 .

Vukašinović, Elvira, Pond, David, Worland, Michael Roger, Kojić, Danijela, Purać, Jelena, Blagojević, Duško, Grubor-Lajšić, Gordana, "Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae)" in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 165, no. 4 (2013):219-225,
https://doi.org/10.1016/j.cbpb.2013.05.001 . .