TY  - CONF
AU  - Bataveljić, Danijela B
AU  - Nikolić, Ljiljana
AU  - Anđus, Pavle R.
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/649
AB  - Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease affecting predominantly motoneurons but with a particular pathological role of non-neuronal cells. Previous research suggested that increased concentration of extracellular potassium could lead to motoneuron dysfunction.
The Na /K -ATPase should regulate the homeostasis of potassium ions and thus help maintain regular neuronal activity. This homeostasis m. he regulated by Nat/K'-ATPase in both neurons and astrocytes. In ordere to reveal the possible role of the Na'/K -ATPase in ALS pathology, we explared the expression of the alphal catalytic subunit of Na/K-ATPase in neurons and astrocytes from different brain regions of the SOD19934 rat model of ALS. Labeling immunofluorescently the alphal isoform of the Na' /K'-ATPase revealed a decreased signal in the trigemi-1 nal nucleus and cerebellum of the ALS rat. Double immunofluorecence with NeuN for neurons showed that the reduced alphal was on the neuronal surface in the trigeminal nucleus. In addition, the alphal ring-like staining of cerebellar granule neurons was lower in ALS as compared to the wild type rat. In comparison to neurons, astrocytes from the wild type rat were characterized with a lower expression of alphal. In the examined brain regions we did not observe a prominent difference in alphal expression between astrocytes of wild Lype and ALS rats. Based on the findings of this study the prominent reduction of Nat/K'-ATPase level observed in neurons and not in astrocytes could contribute to further understaning of impaired ion homeostasis affecting proper neuronal physiology in ALS. The role of glial Na/K*-ATPase needs to be further studied with markers of other alpha subunit isoforms.
PB  - John Wiley and Sons
C3  - 11th European Meeting on Glial Cells in Health and Disease; 2013 Jul 3-6; Berlin, Germany
T1  - Decreased Expression of Alpha1 Subunit of Na(+)/K+-Atpase in the Als Rat Brain
VL  - 61
EP  - S63
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_649
ER  - 

@conference{
author = "Bataveljić, Danijela B and Nikolić, Ljiljana and Anđus, Pavle R.",
year = "2013",
abstract = "Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease affecting predominantly motoneurons but with a particular pathological role of non-neuronal cells. Previous research suggested that increased concentration of extracellular potassium could lead to motoneuron dysfunction.
The Na /K -ATPase should regulate the homeostasis of potassium ions and thus help maintain regular neuronal activity. This homeostasis m. he regulated by Nat/K'-ATPase in both neurons and astrocytes. In ordere to reveal the possible role of the Na'/K -ATPase in ALS pathology, we explared the expression of the alphal catalytic subunit of Na/K-ATPase in neurons and astrocytes from different brain regions of the SOD19934 rat model of ALS. Labeling immunofluorescently the alphal isoform of the Na' /K'-ATPase revealed a decreased signal in the trigemi-1 nal nucleus and cerebellum of the ALS rat. Double immunofluorecence with NeuN for neurons showed that the reduced alphal was on the neuronal surface in the trigeminal nucleus. In addition, the alphal ring-like staining of cerebellar granule neurons was lower in ALS as compared to the wild type rat. In comparison to neurons, astrocytes from the wild type rat were characterized with a lower expression of alphal. In the examined brain regions we did not observe a prominent difference in alphal expression between astrocytes of wild Lype and ALS rats. Based on the findings of this study the prominent reduction of Nat/K'-ATPase level observed in neurons and not in astrocytes could contribute to further understaning of impaired ion homeostasis affecting proper neuronal physiology in ALS. The role of glial Na/K*-ATPase needs to be further studied with markers of other alpha subunit isoforms.",
publisher = "John Wiley and Sons",
journal = "11th European Meeting on Glial Cells in Health and Disease; 2013 Jul 3-6; Berlin, Germany",
title = "Decreased Expression of Alpha1 Subunit of Na(+)/K+-Atpase in the Als Rat Brain",
volume = "61",
pages = "S63",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_649"
}

Bataveljić, D. B., Nikolić, L.,& Anđus, P. R.. (2013). Decreased Expression of Alpha1 Subunit of Na(+)/K+-Atpase in the Als Rat Brain. in 11th European Meeting on Glial Cells in Health and Disease; 2013 Jul 3-6; Berlin, Germany
John Wiley and Sons., 61.
https://hdl.handle.net/21.15107/rcub_ibiss_649

Bataveljić DB, Nikolić L, Anđus PR. Decreased Expression of Alpha1 Subunit of Na(+)/K+-Atpase in the Als Rat Brain. in 11th European Meeting on Glial Cells in Health and Disease; 2013 Jul 3-6; Berlin, Germany. 2013;61:null-S63.
https://hdl.handle.net/21.15107/rcub_ibiss_649 .

Bataveljić, Danijela B, Nikolić, Ljiljana, Anđus, Pavle R., "Decreased Expression of Alpha1 Subunit of Na(+)/K+-Atpase in the Als Rat Brain" in 11th European Meeting on Glial Cells in Health and Disease; 2013 Jul 3-6; Berlin, Germany, 61 (2013),
https://hdl.handle.net/21.15107/rcub_ibiss_649 .

TY  - JOUR
AU  - Bajić, Aleksandar
AU  - Spasić, Mihajlo
AU  - Anđus, Pavle R
AU  - Savić, Danijela
AU  - Parabučki, Ana B.
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasojević, Ivan B
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/959
AB  - The effects of H2O2 are widely studied in cell cultures and other in vitro systems. However, such investigations are performed with the assumption that H2O2 concentration is constant, which may not properly reflect in vivo settings, particularly in redox-turbulent microenvironments such as mitochondria. Here we introduced and tested a novel concept of fluctuating oxidative stress. We treated C6 astroglial cells and primary astrocytes with H2O2, using three regimes of exposure - continuous, as well as fluctuating at low or high rate, and evaluated mitochondrial membrane potential and other parameters of mitochondrial activity - respiration, reducing capacity, and superoxide production, as well as intracellular ATP, intracellular calcium, and NF-kappa B activation. When compared to continuous exposure, fluctuating H2O2 induced a pronounced hyperpolarization in mitochondria, whereas the activity of electron transport chain appears not to be significantly affected. H2O2 provoked a decrease of ATP level and an increase of intracellular calcium concentration, independently of the regime of treatment. However, fluctuating H2O2 induced a specific pattern of large-amplitude fluctuations of calcium concentration. An impact on NF kappa B activation was observed for high rate fluctuations, whereas continuous and low rate fluctuating oxidative stress did not provoke significant effects. Presented results outline the (patho)physiological relevance of redox fluctuations.
T2  - Plos One
T1  - Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia
IS  - 10
VL  - 8
SP  - 111
EP  - na
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_959
ER  - 

@article{
author = "Bajić, Aleksandar and Spasić, Mihajlo and Anđus, Pavle R and Savić, Danijela and Parabučki, Ana B. and Nikolić-Kokić, Aleksandra and Spasojević, Ivan B",
year = "2013",
abstract = "The effects of H2O2 are widely studied in cell cultures and other in vitro systems. However, such investigations are performed with the assumption that H2O2 concentration is constant, which may not properly reflect in vivo settings, particularly in redox-turbulent microenvironments such as mitochondria. Here we introduced and tested a novel concept of fluctuating oxidative stress. We treated C6 astroglial cells and primary astrocytes with H2O2, using three regimes of exposure - continuous, as well as fluctuating at low or high rate, and evaluated mitochondrial membrane potential and other parameters of mitochondrial activity - respiration, reducing capacity, and superoxide production, as well as intracellular ATP, intracellular calcium, and NF-kappa B activation. When compared to continuous exposure, fluctuating H2O2 induced a pronounced hyperpolarization in mitochondria, whereas the activity of electron transport chain appears not to be significantly affected. H2O2 provoked a decrease of ATP level and an increase of intracellular calcium concentration, independently of the regime of treatment. However, fluctuating H2O2 induced a specific pattern of large-amplitude fluctuations of calcium concentration. An impact on NF kappa B activation was observed for high rate fluctuations, whereas continuous and low rate fluctuating oxidative stress did not provoke significant effects. Presented results outline the (patho)physiological relevance of redox fluctuations.",
journal = "Plos One",
title = "Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia",
number = "10",
volume = "8",
pages = "111-na",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_959"
}

Bajić, A., Spasić, M., Anđus, P. R., Savić, D., Parabučki, A. B., Nikolić-Kokić, A.,& Spasojević, I. B.. (2013). Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia. in Plos One, 8(10), 111-na.
https://hdl.handle.net/21.15107/rcub_ibiss_959

Bajić A, Spasić M, Anđus PR, Savić D, Parabučki AB, Nikolić-Kokić A, Spasojević IB. Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia. in Plos One. 2013;8(10):111-na.
https://hdl.handle.net/21.15107/rcub_ibiss_959 .

Bajić, Aleksandar, Spasić, Mihajlo, Anđus, Pavle R, Savić, Danijela, Parabučki, Ana B., Nikolić-Kokić, Aleksandra, Spasojević, Ivan B, "Fluctuating vs. Continuous Exposure to H2O2: The Effects on Mitochondrial Membrane Potential, Intracellular Calcium, and NF-kappa B in Astroglia" in Plos One, 8, no. 10 (2013):111-na,
https://hdl.handle.net/21.15107/rcub_ibiss_959 .

TY  - JOUR
AU  - Nikolić, Ljiljana
AU  - Bataveljić, Danijela B
AU  - Anđus, Pavle
AU  - Nedeljković, Miodrag S
AU  - Todorović, Dajana
AU  - Petković, Branka
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/972
UR  - https://jeb.biologists.org/content/216/18/3531
AB  - Compelling evidence supports the use of a moderate static magnetic field (SMF) for therapeutic purposes. In order to provide insight into the mechanisms underlying SMF treatment, it is essential to examine the cellular responses elicited by therapeutically applied SMF, especially in the nervous system. The Na+/K+ pump, by creating and maintaining the gradient of Na+ and K+ ions across the plasma membrane, regulates the physiological properties of neurons. In this study, we examined the expression of the Na+/K+ pump in the isolated brain-subesophageal ganglion complex of the garden snail Helix pomatia, along with the immunoreactivity and current of the Na+/K+ pump in isolated snail neurons after 15 min exposure to a moderate (10 mT) SMF. Western blot and immunofluorescence analysis revealed that 10 mT SMF did not significantly change the expression of the Na+/K+ pump a-subunit in the snail brain and the neuronal cell body. However, our immunofluorescence data showed that SMF treatment induced a significant increase in the Na+/K+ pump a-subunit expression in the neuronal plasma membrane area. This change in Na+/K+ pump expression was reflected in pump activity as demonstrated by the pump current measurements. Whole-cell patch-clamp recordings from isolated snail neurons revealed that Na+/K+ pump current density was significantly increased after the 10 mT SMF treatment. The SMF-induced increase was different in the two groups of control snail neurons, as defined by the pump current level. The results obtained could represent a physiologically important response of neurons to 10 mT SMF comparable in strength to therapeutic applications.
PB  - The Company of Biologists Ltd.
T2  - Journal of Experimental Biology
T1  - Changes in the expression and current of the Na+/K+ pump in the snail nervous system after exposure to a static magnetic field
IS  - 18
VL  - 216
DO  - 10.1242/jeb.085332
SP  - 3531
EP  - 3541
ER  - 

@article{
author = "Nikolić, Ljiljana and Bataveljić, Danijela B and Anđus, Pavle and Nedeljković, Miodrag S and Todorović, Dajana and Petković, Branka",
year = "2013",
abstract = "Compelling evidence supports the use of a moderate static magnetic field (SMF) for therapeutic purposes. In order to provide insight into the mechanisms underlying SMF treatment, it is essential to examine the cellular responses elicited by therapeutically applied SMF, especially in the nervous system. The Na+/K+ pump, by creating and maintaining the gradient of Na+ and K+ ions across the plasma membrane, regulates the physiological properties of neurons. In this study, we examined the expression of the Na+/K+ pump in the isolated brain-subesophageal ganglion complex of the garden snail Helix pomatia, along with the immunoreactivity and current of the Na+/K+ pump in isolated snail neurons after 15 min exposure to a moderate (10 mT) SMF. Western blot and immunofluorescence analysis revealed that 10 mT SMF did not significantly change the expression of the Na+/K+ pump a-subunit in the snail brain and the neuronal cell body. However, our immunofluorescence data showed that SMF treatment induced a significant increase in the Na+/K+ pump a-subunit expression in the neuronal plasma membrane area. This change in Na+/K+ pump expression was reflected in pump activity as demonstrated by the pump current measurements. Whole-cell patch-clamp recordings from isolated snail neurons revealed that Na+/K+ pump current density was significantly increased after the 10 mT SMF treatment. The SMF-induced increase was different in the two groups of control snail neurons, as defined by the pump current level. The results obtained could represent a physiologically important response of neurons to 10 mT SMF comparable in strength to therapeutic applications.",
publisher = "The Company of Biologists Ltd.",
journal = "Journal of Experimental Biology",
title = "Changes in the expression and current of the Na+/K+ pump in the snail nervous system after exposure to a static magnetic field",
number = "18",
volume = "216",
doi = "10.1242/jeb.085332",
pages = "3531-3541"
}

Nikolić, L., Bataveljić, D. B., Anđus, P., Nedeljković, M. S., Todorović, D.,& Petković, B.. (2013). Changes in the expression and current of the Na+/K+ pump in the snail nervous system after exposure to a static magnetic field. in Journal of Experimental Biology
The Company of Biologists Ltd.., 216(18), 3531-3541.
https://doi.org/10.1242/jeb.085332

Nikolić L, Bataveljić DB, Anđus P, Nedeljković MS, Todorović D, Petković B. Changes in the expression and current of the Na+/K+ pump in the snail nervous system after exposure to a static magnetic field. in Journal of Experimental Biology. 2013;216(18):3531-3541.
doi:10.1242/jeb.085332 .

Nikolić, Ljiljana, Bataveljić, Danijela B, Anđus, Pavle, Nedeljković, Miodrag S, Todorović, Dajana, Petković, Branka, "Changes in the expression and current of the Na+/K+ pump in the snail nervous system after exposure to a static magnetic field" in Journal of Experimental Biology, 216, no. 18 (2013):3531-3541,
https://doi.org/10.1242/jeb.085332 . .

TY  - JOUR
AU  - Jakovcevski, Igor
AU  - Miljković, Đorđe
AU  - Schachner, Melitta
AU  - Anđus, Pavle R
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/652
AB  - In vitro and in vivo studies on the role of tenascins have shown that the two paradigmatic glycoproteins of the tenascin family, tenascin-C (TnC) and tenascin-R (TnR) play important roles in cell proliferation and migration, fate determination, axonal pathfinding, myelination, and synaptic plasticity. As components of the extracellular matrix, both molecules show distinct, but also overlapping dual functions in inhibiting and promoting cell interactions depending on the cell type, developmental stage and molecular microenvironment. They are expressed by neurons and glia as well as, for TnC, by cells of the immune system. The functional relationship between neural and immune cells becomes relevant in acute and chronic nervous system disorders, in particular when the blood brain and blood peripheral nerve barriers are compromised. In this review, we will describe the functional parameters of the two molecules in cell interactions during development and, in the adult, in synaptic activity and plasticity, as well as regeneration after injury, with TnC being conducive for regeneration and TnR being inhibitory for functional recovery. Although not much is known about the role of tenascins in neuroinflammation, we will describe emerging knowledge on the interplay between neural and immune cells in autoimmune diseases, such as multiple sclerosis and polyneuropathies. We will attempt to point out the directions of experimental approaches that we envisage would help gaining insights into the complex interplay of TnC and TnR with the cells that express them in pathological conditions of nervous and immune systems.
T2  - Amino Acids
T1  - Tenascins and inflammation in disorders of the nervous system
IS  - 4
VL  - 44
SP  - null
EP  - 1127
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_652
ER  - 

@article{
author = "Jakovcevski, Igor and Miljković, Đorđe and Schachner, Melitta and Anđus, Pavle R",
year = "2013",
abstract = "In vitro and in vivo studies on the role of tenascins have shown that the two paradigmatic glycoproteins of the tenascin family, tenascin-C (TnC) and tenascin-R (TnR) play important roles in cell proliferation and migration, fate determination, axonal pathfinding, myelination, and synaptic plasticity. As components of the extracellular matrix, both molecules show distinct, but also overlapping dual functions in inhibiting and promoting cell interactions depending on the cell type, developmental stage and molecular microenvironment. They are expressed by neurons and glia as well as, for TnC, by cells of the immune system. The functional relationship between neural and immune cells becomes relevant in acute and chronic nervous system disorders, in particular when the blood brain and blood peripheral nerve barriers are compromised. In this review, we will describe the functional parameters of the two molecules in cell interactions during development and, in the adult, in synaptic activity and plasticity, as well as regeneration after injury, with TnC being conducive for regeneration and TnR being inhibitory for functional recovery. Although not much is known about the role of tenascins in neuroinflammation, we will describe emerging knowledge on the interplay between neural and immune cells in autoimmune diseases, such as multiple sclerosis and polyneuropathies. We will attempt to point out the directions of experimental approaches that we envisage would help gaining insights into the complex interplay of TnC and TnR with the cells that express them in pathological conditions of nervous and immune systems.",
journal = "Amino Acids",
title = "Tenascins and inflammation in disorders of the nervous system",
number = "4",
volume = "44",
pages = "null-1127",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_652"
}

Jakovcevski, I., Miljković, Đ., Schachner, M.,& Anđus, P. R.. (2013). Tenascins and inflammation in disorders of the nervous system. in Amino Acids, 44(4), null-1127.
https://hdl.handle.net/21.15107/rcub_ibiss_652

Jakovcevski I, Miljković Đ, Schachner M, Anđus PR. Tenascins and inflammation in disorders of the nervous system. in Amino Acids. 2013;44(4):null-1127.
https://hdl.handle.net/21.15107/rcub_ibiss_652 .

Jakovcevski, Igor, Miljković, Đorđe, Schachner, Melitta, Anđus, Pavle R, "Tenascins and inflammation in disorders of the nervous system" in Amino Acids, 44, no. 4 (2013):null-1127,
https://hdl.handle.net/21.15107/rcub_ibiss_652 .

TY  - JOUR
AU  - Bataveljić, Danijela B
AU  - Nikolić, Ljiljana
AU  - Milosević, Milena B
AU  - Todorović, Nataša
AU  - Anđus, Pavle R
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1083
AB  - Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease affecting upper and lower motor neurons. Dysfunction and death of motor neurons are closely related to the modified astrocytic environment. Astrocytic endfeet, lining the bloodbrain barrier (BBB), are enriched in two proteins, aquaporin-4 (AQP4) and inwardly rectifying potassium channel (Kir) 4.1. Both channels are important for the maintainance of a functional BBB astrocytic lining. In this study, expression levels of AQP4 and Kir4.1 were for the first time examined in the brainstem and cortex, along with the functional properties of Kir channels in cultured cortical astrocytes of the SOD1G93A rat model of ALS. Western blot analysis showed increased expression of AQP4 and decreased expression of Kir4.1 in the brainstem and cortex of the ALS rat. In addition, higher immunoreactivity of AQP4 and reduced immunolabeling of Kir4.1 in facial and trigeminal nuclei as well as in the motor cortex were also observed. Particularly, the observed changes in the expression of both channels were retained in cultured astrocytes. Furthermore, whole-cell patch-clamp recordings from cultured ALS cortical astrocytes showed a significantly lower Kir current density. Importantly, the potassium uptake current in ALS astrocytes was significantly reduced at all extracellular potassium concentrations. Consequently, the Kir-specific Cs+- and Ba2+-sensitive currents were also decreased. The changes in the studied channels, notably at the upper CNS level, could underline the hampered ability of astrocytes to maintain water and potassium homeostasis, thus affecting the BBB, disturbing the neuronal microenvironment, and causing motoneuronal dysfunction and death. (c) 2012 Wiley Periodicals, Inc.
PB  - Wiley
T2  - Glia
T1  - Changes in the astrocytic aquaporin-4 and inwardly rectifying potassium channel expression in the brain of the amyotrophic lateral sclerosis SOD1G93A rat model
IS  - 12
VL  - 60
DO  - 10.1002/glia.22414
SP  - 1991
EP  - 2003
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1083
ER  - 

@article{
author = "Bataveljić, Danijela B and Nikolić, Ljiljana and Milosević, Milena B and Todorović, Nataša and Anđus, Pavle R",
year = "2012",
abstract = "Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease affecting upper and lower motor neurons. Dysfunction and death of motor neurons are closely related to the modified astrocytic environment. Astrocytic endfeet, lining the bloodbrain barrier (BBB), are enriched in two proteins, aquaporin-4 (AQP4) and inwardly rectifying potassium channel (Kir) 4.1. Both channels are important for the maintainance of a functional BBB astrocytic lining. In this study, expression levels of AQP4 and Kir4.1 were for the first time examined in the brainstem and cortex, along with the functional properties of Kir channels in cultured cortical astrocytes of the SOD1G93A rat model of ALS. Western blot analysis showed increased expression of AQP4 and decreased expression of Kir4.1 in the brainstem and cortex of the ALS rat. In addition, higher immunoreactivity of AQP4 and reduced immunolabeling of Kir4.1 in facial and trigeminal nuclei as well as in the motor cortex were also observed. Particularly, the observed changes in the expression of both channels were retained in cultured astrocytes. Furthermore, whole-cell patch-clamp recordings from cultured ALS cortical astrocytes showed a significantly lower Kir current density. Importantly, the potassium uptake current in ALS astrocytes was significantly reduced at all extracellular potassium concentrations. Consequently, the Kir-specific Cs+- and Ba2+-sensitive currents were also decreased. The changes in the studied channels, notably at the upper CNS level, could underline the hampered ability of astrocytes to maintain water and potassium homeostasis, thus affecting the BBB, disturbing the neuronal microenvironment, and causing motoneuronal dysfunction and death. (c) 2012 Wiley Periodicals, Inc.",
publisher = "Wiley",
journal = "Glia",
title = "Changes in the astrocytic aquaporin-4 and inwardly rectifying potassium channel expression in the brain of the amyotrophic lateral sclerosis SOD1G93A rat model",
number = "12",
volume = "60",
doi = "10.1002/glia.22414",
pages = "1991-2003",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1083"
}

Bataveljić, D. B., Nikolić, L., Milosević, M. B., Todorović, N.,& Anđus, P. R.. (2012). Changes in the astrocytic aquaporin-4 and inwardly rectifying potassium channel expression in the brain of the amyotrophic lateral sclerosis SOD1G93A rat model. in Glia
Wiley., 60(12), 1991-2003.
https://doi.org/10.1002/glia.22414
https://hdl.handle.net/21.15107/rcub_ibiss_1083

Bataveljić DB, Nikolić L, Milosević MB, Todorović N, Anđus PR. Changes in the astrocytic aquaporin-4 and inwardly rectifying potassium channel expression in the brain of the amyotrophic lateral sclerosis SOD1G93A rat model. in Glia. 2012;60(12):1991-2003.
doi:10.1002/glia.22414
https://hdl.handle.net/21.15107/rcub_ibiss_1083 .

Bataveljić, Danijela B, Nikolić, Ljiljana, Milosević, Milena B, Todorović, Nataša, Anđus, Pavle R, "Changes in the astrocytic aquaporin-4 and inwardly rectifying potassium channel expression in the brain of the amyotrophic lateral sclerosis SOD1G93A rat model" in Glia, 60, no. 12 (2012):1991-2003,
https://doi.org/10.1002/glia.22414 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1083 .

TY  - JOUR
AU  - Brisevac, Dusica
AU  - Bjelobaba, Ivana
AU  - Bajić, Aleksandar
AU  - Clarner, Tim
AU  - Stojiljković, Mirjana B
AU  - Beyer, Cordian
AU  - Anđus, Pavle R
AU  - Kipp, Markus
AU  - Nedeljković, Nadezda N
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1098
AB  - Ecto-5'-nucleotidase (e-5NT) is a cell-surface located, rate-limiting enzyme in the extracellular metabolism of ATP, catalyzing the final step of the conversion of AMP to adenosine. Since this enzyme shifts the balance from pro-inflammatory ATP to anti-inflammatory adenosine, it is considered to be an important regulator of inflammation. Although up-regulation of e-5NT was repeatedly reported in several in vivo models of brain injury, the regulation of its expression and function remains largely unknown. We have studied effects of several pro-inflammatory factors, namely, bacterial endotoxin lipopolysaccharide (LPS), tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), glutamate (Glu) and hydrogen peroxide (H2O2) on e-5NT (i) activity, (ii) mRNA expression and (iii) membrane protein abundance in primary cultured cortical astrocytes. We are clearly able to demonstrate a stimulus-specific regulation of the e-5NT pathway. IFN-gamma, LPS, Glu and H2O2 decrease, while TNF-alpha increases e-5NT activity. The analysis of e-5NT gene expression and e-5NT membrane protein levels revealed that tested factors regulate e-5NT at different levels and by employing different mechanisms. In summary, we provide evidence that e-5NT activity is tightly regulated in a stimulus-specific manner. (C) 2012 Elsevier Ltd. All rights reserved.
T2  - Neurochemistry International
T1  - Regulation of ecto-5 '-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors
IS  - 5
VL  - 61
SP  - 406
EP  - 688
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1098
ER  - 

@article{
author = "Brisevac, Dusica and Bjelobaba, Ivana and Bajić, Aleksandar and Clarner, Tim and Stojiljković, Mirjana B and Beyer, Cordian and Anđus, Pavle R and Kipp, Markus and Nedeljković, Nadezda N",
year = "2012",
abstract = "Ecto-5'-nucleotidase (e-5NT) is a cell-surface located, rate-limiting enzyme in the extracellular metabolism of ATP, catalyzing the final step of the conversion of AMP to adenosine. Since this enzyme shifts the balance from pro-inflammatory ATP to anti-inflammatory adenosine, it is considered to be an important regulator of inflammation. Although up-regulation of e-5NT was repeatedly reported in several in vivo models of brain injury, the regulation of its expression and function remains largely unknown. We have studied effects of several pro-inflammatory factors, namely, bacterial endotoxin lipopolysaccharide (LPS), tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), glutamate (Glu) and hydrogen peroxide (H2O2) on e-5NT (i) activity, (ii) mRNA expression and (iii) membrane protein abundance in primary cultured cortical astrocytes. We are clearly able to demonstrate a stimulus-specific regulation of the e-5NT pathway. IFN-gamma, LPS, Glu and H2O2 decrease, while TNF-alpha increases e-5NT activity. The analysis of e-5NT gene expression and e-5NT membrane protein levels revealed that tested factors regulate e-5NT at different levels and by employing different mechanisms. In summary, we provide evidence that e-5NT activity is tightly regulated in a stimulus-specific manner. (C) 2012 Elsevier Ltd. All rights reserved.",
journal = "Neurochemistry International",
title = "Regulation of ecto-5 '-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors",
number = "5",
volume = "61",
pages = "406-688",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1098"
}

Brisevac, D., Bjelobaba, I., Bajić, A., Clarner, T., Stojiljković, M. B., Beyer, C., Anđus, P. R., Kipp, M.,& Nedeljković, N. N.. (2012). Regulation of ecto-5 '-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors. in Neurochemistry International, 61(5), 406-688.
https://hdl.handle.net/21.15107/rcub_ibiss_1098

Brisevac D, Bjelobaba I, Bajić A, Clarner T, Stojiljković MB, Beyer C, Anđus PR, Kipp M, Nedeljković NN. Regulation of ecto-5 '-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors. in Neurochemistry International. 2012;61(5):406-688.
https://hdl.handle.net/21.15107/rcub_ibiss_1098 .

Brisevac, Dusica, Bjelobaba, Ivana, Bajić, Aleksandar, Clarner, Tim, Stojiljković, Mirjana B, Beyer, Cordian, Anđus, Pavle R, Kipp, Markus, Nedeljković, Nadezda N, "Regulation of ecto-5 '-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors" in Neurochemistry International, 61, no. 5 (2012):406-688,
https://hdl.handle.net/21.15107/rcub_ibiss_1098 .

TY  - CONF
AU  - Bataveljić, Danijela B
AU  - Korenić, Andrej
AU  - Nikolić, Ljiljana
AU  - Todorović, Nataša
AU  - Moghaddam-Amiry, M
AU  - Anđus, Pavle R
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1260
AB  - Combined MRI and immunofluorescence studies on the brain of the transgenic hSOD1 G93A ALS rat model reveled astrogliosis near dilated lateral ventricles and blood-brain barrier (BBB) compromise. It was thus of particular interest to check the expression of glial aquaporins known to be specifically present in the astroglial endfeet of the BBB. Immunoflu-orescence revealed a more abundant expression of aquaporin 4 (AQP4) in the brainstem and cerebral cortex of ALS rats. Immunogold EM studies
also revealed a supramolecular change in the distribution of AQP4 in
astrocytic endfeet ensheathing blood vessels. A reverse expression was also observed for astrocytic AQP4 versus the Kir 4.1 channel-the two proteins known to form aggregates. Preliminary electrophysiological data on astrocytes in culture showed a different behavior of the Kir current as compared to the wild type. Thus, a role of glia in the demise of the BBB in the ALS brain was suggested with the AQP4 and Kir4. 1 complex being the molecular target for possible therapies.
PB  - John Wiley and Sons
C3  - 10th European meeting on Glial Cells in Health and Disease; 2011 Sep 13-17; Prague, Czech Republic
T1  - The Demise of the Blood-Brain Barrier in Amyotrophic Lateral Sclerosis and the Role of Astrocytes
EP  - S83
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1260
ER  - 

@conference{
author = "Bataveljić, Danijela B and Korenić, Andrej and Nikolić, Ljiljana and Todorović, Nataša and Moghaddam-Amiry, M and Anđus, Pavle R",
year = "2011",
abstract = "Combined MRI and immunofluorescence studies on the brain of the transgenic hSOD1 G93A ALS rat model reveled astrogliosis near dilated lateral ventricles and blood-brain barrier (BBB) compromise. It was thus of particular interest to check the expression of glial aquaporins known to be specifically present in the astroglial endfeet of the BBB. Immunoflu-orescence revealed a more abundant expression of aquaporin 4 (AQP4) in the brainstem and cerebral cortex of ALS rats. Immunogold EM studies
also revealed a supramolecular change in the distribution of AQP4 in
astrocytic endfeet ensheathing blood vessels. A reverse expression was also observed for astrocytic AQP4 versus the Kir 4.1 channel-the two proteins known to form aggregates. Preliminary electrophysiological data on astrocytes in culture showed a different behavior of the Kir current as compared to the wild type. Thus, a role of glia in the demise of the BBB in the ALS brain was suggested with the AQP4 and Kir4. 1 complex being the molecular target for possible therapies.",
publisher = "John Wiley and Sons",
journal = "10th European meeting on Glial Cells in Health and Disease; 2011 Sep 13-17; Prague, Czech Republic",
title = "The Demise of the Blood-Brain Barrier in Amyotrophic Lateral Sclerosis and the Role of Astrocytes",
pages = "S83",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1260"
}

Bataveljić, D. B., Korenić, A., Nikolić, L., Todorović, N., Moghaddam-Amiry, M.,& Anđus, P. R.. (2011). The Demise of the Blood-Brain Barrier in Amyotrophic Lateral Sclerosis and the Role of Astrocytes. in 10th European meeting on Glial Cells in Health and Disease; 2011 Sep 13-17; Prague, Czech Republic
John Wiley and Sons..
https://hdl.handle.net/21.15107/rcub_ibiss_1260

Bataveljić DB, Korenić A, Nikolić L, Todorović N, Moghaddam-Amiry M, Anđus PR. The Demise of the Blood-Brain Barrier in Amyotrophic Lateral Sclerosis and the Role of Astrocytes. in 10th European meeting on Glial Cells in Health and Disease; 2011 Sep 13-17; Prague, Czech Republic. 2011;:null-S83.
https://hdl.handle.net/21.15107/rcub_ibiss_1260 .

Bataveljić, Danijela B, Korenić, Andrej, Nikolić, Ljiljana, Todorović, Nataša, Moghaddam-Amiry, M, Anđus, Pavle R, "The Demise of the Blood-Brain Barrier in Amyotrophic Lateral Sclerosis and the Role of Astrocytes" in 10th European meeting on Glial Cells in Health and Disease; 2011 Sep 13-17; Prague, Czech Republic (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1260 .