TY  - JOUR
AU  - Bizarro, Ana
AU  - Ferreira, Isabel C. F. R.
AU  - Soković, Marina
AU  - van Griensven, Leo J. L. D.
AU  - Sousa, Diana
AU  - Vasconcelos, M. Helena
AU  - Lima, Raquel T.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1918
AB  - Cordyceps militaris (L.) Link, an edible entomopathogenic fungus widely
   used in traditional Chinese medicine, has numerous potential medicinal
   properties including antitumor activity. The methanolic extract of C.
   militaris fruiting body was recently shown to have tumor cell growth
   inhibitory activity in several human tumor cell lines. Nonetheless, the
   mechanism of action involved is still not known. This work aimed at
   further studying the effect of the methanolic extract of C. militaris
   regarding its antitumor mechanism of action, using the non-small cell
   lung cancer cell line (NCI-H460) as a model. Results showed that
   treatment with the extract decreased cellular proliferation, induced
   cell cycle arrest at G0/G1 and increased apoptosis. In addition, the
   extract increased the levels of p53 and p21. Moreover, an increase in
   p-H2A.X and 53BP1 levels, together with an increase in the number of
   53BP1 foci/cell (all indicative of DNA damage), were also observed after
   treatment with the extract. This work suggests that this extract
   affected NCI-H460 cellular viability through a mechanism involving DNA
   damage and p53 activation. This further supports the potential of this
   extract as a source of bioactive compounds, which may be used in
   anticancer strategies.
T2  - Molecules
T1  - Cordyceps militaris (L.) Link Fruiting Body Reduces the Growth of a
 Non-Small Cell Lung Cancer Cell Line by Increasing Cellular Levels of
 p53 and p21
IS  - 8
VL  - 20
DO  - 10.3390/molecules200813927
SP  - 13927
EP  - 13940
ER  - 

@article{
author = "Bizarro, Ana and Ferreira, Isabel C. F. R. and Soković, Marina and van Griensven, Leo J. L. D. and Sousa, Diana and Vasconcelos, M. Helena and Lima, Raquel T.",
year = "2015",
abstract = "Cordyceps militaris (L.) Link, an edible entomopathogenic fungus widely
   used in traditional Chinese medicine, has numerous potential medicinal
   properties including antitumor activity. The methanolic extract of C.
   militaris fruiting body was recently shown to have tumor cell growth
   inhibitory activity in several human tumor cell lines. Nonetheless, the
   mechanism of action involved is still not known. This work aimed at
   further studying the effect of the methanolic extract of C. militaris
   regarding its antitumor mechanism of action, using the non-small cell
   lung cancer cell line (NCI-H460) as a model. Results showed that
   treatment with the extract decreased cellular proliferation, induced
   cell cycle arrest at G0/G1 and increased apoptosis. In addition, the
   extract increased the levels of p53 and p21. Moreover, an increase in
   p-H2A.X and 53BP1 levels, together with an increase in the number of
   53BP1 foci/cell (all indicative of DNA damage), were also observed after
   treatment with the extract. This work suggests that this extract
   affected NCI-H460 cellular viability through a mechanism involving DNA
   damage and p53 activation. This further supports the potential of this
   extract as a source of bioactive compounds, which may be used in
   anticancer strategies.",
journal = "Molecules",
title = "Cordyceps militaris (L.) Link Fruiting Body Reduces the Growth of a
 Non-Small Cell Lung Cancer Cell Line by Increasing Cellular Levels of
 p53 and p21",
number = "8",
volume = "20",
doi = "10.3390/molecules200813927",
pages = "13927-13940"
}

Bizarro, A., Ferreira, I. C. F. R., Soković, M., van Griensven, L. J. L. D., Sousa, D., Vasconcelos, M. H.,& Lima, R. T.. (2015). Cordyceps militaris (L.) Link Fruiting Body Reduces the Growth of a
 Non-Small Cell Lung Cancer Cell Line by Increasing Cellular Levels of
 p53 and p21. in Molecules, 20(8), 13927-13940.
https://doi.org/10.3390/molecules200813927

Bizarro A, Ferreira ICFR, Soković M, van Griensven LJLD, Sousa D, Vasconcelos MH, Lima RT. Cordyceps militaris (L.) Link Fruiting Body Reduces the Growth of a
 Non-Small Cell Lung Cancer Cell Line by Increasing Cellular Levels of
 p53 and p21. in Molecules. 2015;20(8):13927-13940.
doi:10.3390/molecules200813927 .

Bizarro, Ana, Ferreira, Isabel C. F. R., Soković, Marina, van Griensven, Leo J. L. D., Sousa, Diana, Vasconcelos, M. Helena, Lima, Raquel T., "Cordyceps militaris (L.) Link Fruiting Body Reduces the Growth of a
 Non-Small Cell Lung Cancer Cell Line by Increasing Cellular Levels of
 p53 and p21" in Molecules, 20, no. 8 (2015):13927-13940,
https://doi.org/10.3390/molecules200813927 . .