TY  - JOUR
AU  - Radovanović, Tijana
AU  - Nasia, Mohammed
AU  - Krizmanić, Imre I.
AU  - Prokić, Marko
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Borković Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2017
UR  - http://doi.wiley.com/10.1002/etc.3849
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3418
AB  - Pyrethroids are synthetic insecticides widely used in agriculture, public health, and veterinary medicine. Deltamethrin, a type II pyrethroid insecticide, has attracted particular attention because of its frequent use. The mechanisms of the toxicity of most pesticides (including pyrethroids) in nontarget organisms is linked to the production of free radicals, oxidative stress induction, increased lipid peroxidation, and disruption of the total antioxidant potential. The aim of the present study was to investigate the effects of acute toxicity of 3 different concentrations (8, 16, and 32 mg/kg body wt) of orally applied deltamethrin after 96 h of treatment. Some of the front-line oxidative stress parameters as well as cholinesterase (ChE) activity and cytochrome P4501A (CYP1A) expression in the liver, muscle, skin, and gastrointestinal tissue of the adult green toad Bufotes viridis were determined. Compared with the control group, the activity of catalase and glutathione reductase was increased in the liver and skin, while the concentration of sulfhydryl groups was reduced. In the liver and muscle, concentrations of thiobarbituric reactive substances were increased, as well as liver CYP1A expression. In the muscle and skin, glutathione-S-transferase activity was higher in treated toads. The oxidative stress parameters examined were affected by different deltamethrin concentrations. We conclude that the assessed parameters represent good biomarkers of pesticide-induced oxidative stress. Environ Toxicol Chem 2017;36:2814-2822. © 2017 SETAC.
T2  - Environmental Toxicology and Chemistry
T1  - Sublethal effects of the pyrethroid insecticide deltamethrin on oxidative stress parameters in green toad (Bufotes viridis L.).
IS  - 10
VL  - 36
DO  - 10.1002/etc.3849
SP  - 2814
EP  - 2822
ER  - 

@article{
author = "Radovanović, Tijana and Nasia, Mohammed and Krizmanić, Imre I. and Prokić, Marko and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Borković Mitić, Slavica and Pavlović, Slađan and Saičić, Zorica",
year = "2017",
abstract = "Pyrethroids are synthetic insecticides widely used in agriculture, public health, and veterinary medicine. Deltamethrin, a type II pyrethroid insecticide, has attracted particular attention because of its frequent use. The mechanisms of the toxicity of most pesticides (including pyrethroids) in nontarget organisms is linked to the production of free radicals, oxidative stress induction, increased lipid peroxidation, and disruption of the total antioxidant potential. The aim of the present study was to investigate the effects of acute toxicity of 3 different concentrations (8, 16, and 32 mg/kg body wt) of orally applied deltamethrin after 96 h of treatment. Some of the front-line oxidative stress parameters as well as cholinesterase (ChE) activity and cytochrome P4501A (CYP1A) expression in the liver, muscle, skin, and gastrointestinal tissue of the adult green toad Bufotes viridis were determined. Compared with the control group, the activity of catalase and glutathione reductase was increased in the liver and skin, while the concentration of sulfhydryl groups was reduced. In the liver and muscle, concentrations of thiobarbituric reactive substances were increased, as well as liver CYP1A expression. In the muscle and skin, glutathione-S-transferase activity was higher in treated toads. The oxidative stress parameters examined were affected by different deltamethrin concentrations. We conclude that the assessed parameters represent good biomarkers of pesticide-induced oxidative stress. Environ Toxicol Chem 2017;36:2814-2822. © 2017 SETAC.",
journal = "Environmental Toxicology and Chemistry",
title = "Sublethal effects of the pyrethroid insecticide deltamethrin on oxidative stress parameters in green toad (Bufotes viridis L.).",
number = "10",
volume = "36",
doi = "10.1002/etc.3849",
pages = "2814-2822"
}

Radovanović, T., Nasia, M., Krizmanić, I. I., Prokić, M., Gavrić, J., Despotović, S., Gavrilović, B., Borković Mitić, S., Pavlović, S.,& Saičić, Z.. (2017). Sublethal effects of the pyrethroid insecticide deltamethrin on oxidative stress parameters in green toad (Bufotes viridis L.).. in Environmental Toxicology and Chemistry, 36(10), 2814-2822.
https://doi.org/10.1002/etc.3849

Radovanović T, Nasia M, Krizmanić II, Prokić M, Gavrić J, Despotović S, Gavrilović B, Borković Mitić S, Pavlović S, Saičić Z. Sublethal effects of the pyrethroid insecticide deltamethrin on oxidative stress parameters in green toad (Bufotes viridis L.).. in Environmental Toxicology and Chemistry. 2017;36(10):2814-2822.
doi:10.1002/etc.3849 .

Radovanović, Tijana, Nasia, Mohammed, Krizmanić, Imre I., Prokić, Marko, Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Borković Mitić, Slavica, Pavlović, Slađan, Saičić, Zorica, "Sublethal effects of the pyrethroid insecticide deltamethrin on oxidative stress parameters in green toad (Bufotes viridis L.)." in Environmental Toxicology and Chemistry, 36, no. 10 (2017):2814-2822,
https://doi.org/10.1002/etc.3849 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Borković Mitić, Slavica
AU  - Krizmanić, Imre I.
AU  - Mutić, Jelena J.
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S1532045617301370
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2827
AB  - In spring, frogs from temperate regions are faced with arousal-induced oxidative stress and exposure to various xenobiotics from the environment. The question is whether pollutants can significantly modify the antioxidative defense system (AOS) response of hibernators during recovery from hibernation. If this assumption is true, we would then expect different patterns of seasonal variations in the AOS between individuals exposed to different levels of pollution. To examine this assumption, we determined the relationship between seasonal variations of accumulated metals and AOS parameters in the skin and muscle of two frog species from the Pelophylax esculentus complex (P. ridibundus and P. esculentus) inhabiting two localities (the Danube-Tisza-Danube canal and the Ponjavica River) with different levels of pollution during pre- and post-hibernation periods, respectively autumn and spring. Our results showed that even though there were differences in the concentrations of accumulated metals and AOS parameters between localities and species, the frogs displayed almost the same patterns of AOS variations during seasons, with a higher AOS response observed in spring. The parameters SH groups, GSH, GR and SOD had been contributed most rather than others. Our findings indicate that oxidative stress during the post-hibernation period was mainly caused by the organisms’ recovery from hibernation, as the result of natural selection acting on the AOS, and that the accumulated metals did not significantly modify the AOS response. The present study provides new insight into the biological and physiological cellular responses of frogs to arousal stress.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Oxidative stress parameters in two Pelophylax esculentus complex frogs during pre- and post-hibernation: Arousal vs heavy metals
VL  - 202
DO  - 10.1016/j.cbpc.2017.07.006
SP  - 19
EP  - 25
ER  - 

@article{
author = "Prokić, Marko and Borković Mitić, Slavica and Krizmanić, Imre I. and Mutić, Jelena J. and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Pavlović, Slađan and Saičić, Zorica",
year = "2017",
abstract = "In spring, frogs from temperate regions are faced with arousal-induced oxidative stress and exposure to various xenobiotics from the environment. The question is whether pollutants can significantly modify the antioxidative defense system (AOS) response of hibernators during recovery from hibernation. If this assumption is true, we would then expect different patterns of seasonal variations in the AOS between individuals exposed to different levels of pollution. To examine this assumption, we determined the relationship between seasonal variations of accumulated metals and AOS parameters in the skin and muscle of two frog species from the Pelophylax esculentus complex (P. ridibundus and P. esculentus) inhabiting two localities (the Danube-Tisza-Danube canal and the Ponjavica River) with different levels of pollution during pre- and post-hibernation periods, respectively autumn and spring. Our results showed that even though there were differences in the concentrations of accumulated metals and AOS parameters between localities and species, the frogs displayed almost the same patterns of AOS variations during seasons, with a higher AOS response observed in spring. The parameters SH groups, GSH, GR and SOD had been contributed most rather than others. Our findings indicate that oxidative stress during the post-hibernation period was mainly caused by the organisms’ recovery from hibernation, as the result of natural selection acting on the AOS, and that the accumulated metals did not significantly modify the AOS response. The present study provides new insight into the biological and physiological cellular responses of frogs to arousal stress.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Oxidative stress parameters in two Pelophylax esculentus complex frogs during pre- and post-hibernation: Arousal vs heavy metals",
volume = "202",
doi = "10.1016/j.cbpc.2017.07.006",
pages = "19-25"
}

Prokić, M., Borković Mitić, S., Krizmanić, I. I., Mutić, J. J., Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Pavlović, S.,& Saičić, Z.. (2017). Oxidative stress parameters in two Pelophylax esculentus complex frogs during pre- and post-hibernation: Arousal vs heavy metals. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 202, 19-25.
https://doi.org/10.1016/j.cbpc.2017.07.006

Prokić M, Borković Mitić S, Krizmanić II, Mutić JJ, Gavrić J, Despotović S, Gavrilović B, Radovanović T, Pavlović S, Saičić Z. Oxidative stress parameters in two Pelophylax esculentus complex frogs during pre- and post-hibernation: Arousal vs heavy metals. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2017;202:19-25.
doi:10.1016/j.cbpc.2017.07.006 .

Prokić, Marko, Borković Mitić, Slavica, Krizmanić, Imre I., Mutić, Jelena J., Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Pavlović, Slađan, Saičić, Zorica, "Oxidative stress parameters in two Pelophylax esculentus complex frogs during pre- and post-hibernation: Arousal vs heavy metals" in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 202 (2017):19-25,
https://doi.org/10.1016/j.cbpc.2017.07.006 . .

TY  - JOUR
AU  - Borković Mitić, Slavica
AU  - Prokić, Marko
AU  - Krizmanić, Imre I.
AU  - Mutić, Jelena
AU  - Trifković, Jelena
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Pavlović, Slađan
AU  - Saičić, Zorica
PY  - 2016
UR  - http://www.springer.com/tdm
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2468
AB  - To understand the effect of metals on the marsh frog Pelophylax ridibundus and the possible environment-induced changes in oxidative stress enzymes, we determined the concentrations of 18 metals: Al, As, Ba, Ca, Cd, Co, Cr, Cu, Fe, Ga, Hg, In, Li, Mn, Ni, Pb, Sr, and Zn, in the tissues (liver, skin, and muscle) and water samples collected from different locations in Serbia. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST), acetylcholinesterase (AChE), and changes in concentrations of reduced glutathione (GSH) and sulfhydryl groups (SH) were analyzed in the tissues of the sampled frogs. The concentrations of Al, Cd, Co, Cr, Cu, Fe, Ga, Hg, and Ni were highest in the liver, whereas those of Ba, Ca, Li, Mn, Pb, Sr, and Zn were highest in the skin. Hg correlated positively with liver SOD (in frogs from Danube-Tisza-Danube Canal (DTD)), muscle CAT (DTD), and muscle GST Ponjavica River (PO); Pb demonstrated a strong positive correlation with liver GR in frogs from Mt. Fruška Gora (FG); Cd only exhibited a positive correlation with AChE in the skin of frogs from DTD. In the skin, Zn correlated positively with AChE (DTD), SH groups (PO), and CAT (FG), and negatively with CAT, GST, and SH in the liver of frogs from DTD. Examination of these oxidative stress biomarkers, together with analysis of metal accumulation in the liver and skin of marsh frogs, provides a powerful tool for the assessment of metal pollution.
PB  - Springer Nature
T2  - Environmental Science and Pollution Research
T1  - Biomarkers of oxidative stress and metal accumulation in marsh frog (Pelophylax ridibundus)
IS  - 10
VL  - 23
DO  - 10.13039/501100004564
SP  - 9649
EP  - 9659
ER  - 

@article{
author = "Borković Mitić, Slavica and Prokić, Marko and Krizmanić, Imre I. and Mutić, Jelena and Trifković, Jelena and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Pavlović, Slađan and Saičić, Zorica",
year = "2016",
abstract = "To understand the effect of metals on the marsh frog Pelophylax ridibundus and the possible environment-induced changes in oxidative stress enzymes, we determined the concentrations of 18 metals: Al, As, Ba, Ca, Cd, Co, Cr, Cu, Fe, Ga, Hg, In, Li, Mn, Ni, Pb, Sr, and Zn, in the tissues (liver, skin, and muscle) and water samples collected from different locations in Serbia. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST), acetylcholinesterase (AChE), and changes in concentrations of reduced glutathione (GSH) and sulfhydryl groups (SH) were analyzed in the tissues of the sampled frogs. The concentrations of Al, Cd, Co, Cr, Cu, Fe, Ga, Hg, and Ni were highest in the liver, whereas those of Ba, Ca, Li, Mn, Pb, Sr, and Zn were highest in the skin. Hg correlated positively with liver SOD (in frogs from Danube-Tisza-Danube Canal (DTD)), muscle CAT (DTD), and muscle GST Ponjavica River (PO); Pb demonstrated a strong positive correlation with liver GR in frogs from Mt. Fruška Gora (FG); Cd only exhibited a positive correlation with AChE in the skin of frogs from DTD. In the skin, Zn correlated positively with AChE (DTD), SH groups (PO), and CAT (FG), and negatively with CAT, GST, and SH in the liver of frogs from DTD. Examination of these oxidative stress biomarkers, together with analysis of metal accumulation in the liver and skin of marsh frogs, provides a powerful tool for the assessment of metal pollution.",
publisher = "Springer Nature",
journal = "Environmental Science and Pollution Research",
title = "Biomarkers of oxidative stress and metal accumulation in marsh frog (Pelophylax ridibundus)",
number = "10",
volume = "23",
doi = "10.13039/501100004564",
pages = "9649-9659"
}

Borković Mitić, S., Prokić, M., Krizmanić, I. I., Mutić, J., Trifković, J., Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Pavlović, S.,& Saičić, Z.. (2016). Biomarkers of oxidative stress and metal accumulation in marsh frog (Pelophylax ridibundus). in Environmental Science and Pollution Research
Springer Nature., 23(10), 9649-9659.
https://doi.org/10.13039/501100004564

Borković Mitić S, Prokić M, Krizmanić II, Mutić J, Trifković J, Gavrić J, Despotović S, Gavrilović B, Radovanović T, Pavlović S, Saičić Z. Biomarkers of oxidative stress and metal accumulation in marsh frog (Pelophylax ridibundus). in Environmental Science and Pollution Research. 2016;23(10):9649-9659.
doi:10.13039/501100004564 .

Borković Mitić, Slavica, Prokić, Marko, Krizmanić, Imre I., Mutić, Jelena, Trifković, Jelena, Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Pavlović, Slađan, Saičić, Zorica, "Biomarkers of oxidative stress and metal accumulation in marsh frog (Pelophylax ridibundus)" in Environmental Science and Pollution Research, 23, no. 10 (2016):9649-9659,
https://doi.org/10.13039/501100004564 . .