TY  - JOUR
AU  - Nikolić, Milos MN
AU  - Marković, Tatjana
AU  - Mojović, Milos D
AU  - Pejin, Boris
AU  - Savić, Aleksandar G
AU  - Perić, Tamara O
AU  - Marković, Dejan Lj
AU  - Stević, Tatjana R
AU  - Soković, Marina
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/982
AB  - The aim of this study was to examine chemical composition and biological activity of Gaultheria procumbens L essential oil (EO) against food spoilage and oral microorganisms. The components of EO were identified by GC-MS. Antimicrobial activity was determined against food spoilage (five bacteria and six fungal species) and oral microorganisms (eight bacteria and thirty two fungal species) by microdilution and microplate biofilm assay, antioxidant activity was tested using the persistent free radical 2,2-diphenyl-1-picryl hydrazyl (DPPH), while antiradical activity was examined by fluorescence spectroscopy and electron paramagnetic resonance spectroscopy (EPR). GC-MS analysis showed that methyl salicylate (96.90%) was the main component of the oil. Essential oil inhibited the growth of all microorganisms tested, i.e. food and oral bacteria and fungi, respectively (MIC 0.18-3.00 mg/ml and MBC 1.25-4.00 mg/ml; MIC 0.73-5.00 mg/ml and MFC 2.92-26.67 mg/ml); The oil effectively inhibited the biofilm formation of oral Streptococcus mutans and Candida albicans as well (MIC 25.00 MBC 50.00 mg/ml; MIC 12.50, MFC 50.00 mg/ml). In addition, oil exhibited a dose-dependent DPPH-radical-scavenging activity with IC50 value of 30.61 mg/ml. The specific fluorescence probe 2-[6-(4'-amino)phenoxy-3H-xanten-3-on-9-yl] benzoic acid (APP) and the the spin trap 5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO), capable for simultaneous detection of different free radical specie were used in antiradical activity of the oil measurements. Oil showed a moderate antiradical activity, reducing quantity of produced hydroxyl radicals to about 20% of initial value. This study succeeds in creating directly comparable and quantitative data for the oil unsufficiently examined so far. (C) 2013 Elsevier B.V. All rights reserved.
T2  - Industrial Crops and Products
T1  - Chemical composition and biological activity of Gaultheria procumbens L. essential oil
IS  - null
VL  - 49
SP  - 344
EP  - 567
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_982
ER  - 

@article{
author = "Nikolić, Milos MN and Marković, Tatjana and Mojović, Milos D and Pejin, Boris and Savić, Aleksandar G and Perić, Tamara O and Marković, Dejan Lj and Stević, Tatjana R and Soković, Marina",
year = "2013",
abstract = "The aim of this study was to examine chemical composition and biological activity of Gaultheria procumbens L essential oil (EO) against food spoilage and oral microorganisms. The components of EO were identified by GC-MS. Antimicrobial activity was determined against food spoilage (five bacteria and six fungal species) and oral microorganisms (eight bacteria and thirty two fungal species) by microdilution and microplate biofilm assay, antioxidant activity was tested using the persistent free radical 2,2-diphenyl-1-picryl hydrazyl (DPPH), while antiradical activity was examined by fluorescence spectroscopy and electron paramagnetic resonance spectroscopy (EPR). GC-MS analysis showed that methyl salicylate (96.90%) was the main component of the oil. Essential oil inhibited the growth of all microorganisms tested, i.e. food and oral bacteria and fungi, respectively (MIC 0.18-3.00 mg/ml and MBC 1.25-4.00 mg/ml; MIC 0.73-5.00 mg/ml and MFC 2.92-26.67 mg/ml); The oil effectively inhibited the biofilm formation of oral Streptococcus mutans and Candida albicans as well (MIC 25.00 MBC 50.00 mg/ml; MIC 12.50, MFC 50.00 mg/ml). In addition, oil exhibited a dose-dependent DPPH-radical-scavenging activity with IC50 value of 30.61 mg/ml. The specific fluorescence probe 2-[6-(4'-amino)phenoxy-3H-xanten-3-on-9-yl] benzoic acid (APP) and the the spin trap 5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO), capable for simultaneous detection of different free radical specie were used in antiradical activity of the oil measurements. Oil showed a moderate antiradical activity, reducing quantity of produced hydroxyl radicals to about 20% of initial value. This study succeeds in creating directly comparable and quantitative data for the oil unsufficiently examined so far. (C) 2013 Elsevier B.V. All rights reserved.",
journal = "Industrial Crops and Products",
title = "Chemical composition and biological activity of Gaultheria procumbens L. essential oil",
number = "null",
volume = "49",
pages = "344-567",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_982"
}

Nikolić, M. M., Marković, T., Mojović, M. D., Pejin, B., Savić, A. G., Perić, T. O., Marković, D. L., Stević, T. R.,& Soković, M.. (2013). Chemical composition and biological activity of Gaultheria procumbens L. essential oil. in Industrial Crops and Products, 49(null), 344-567.
https://hdl.handle.net/21.15107/rcub_ibiss_982

Nikolić MM, Marković T, Mojović MD, Pejin B, Savić AG, Perić TO, Marković DL, Stević TR, Soković M. Chemical composition and biological activity of Gaultheria procumbens L. essential oil. in Industrial Crops and Products. 2013;49(null):344-567.
https://hdl.handle.net/21.15107/rcub_ibiss_982 .

Nikolić, Milos MN, Marković, Tatjana, Mojović, Milos D, Pejin, Boris, Savić, Aleksandar G, Perić, Tamara O, Marković, Dejan Lj, Stević, Tatjana R, Soković, Marina, "Chemical composition and biological activity of Gaultheria procumbens L. essential oil" in Industrial Crops and Products, 49, no. null (2013):344-567,
https://hdl.handle.net/21.15107/rcub_ibiss_982 .

TY  - JOUR
AU  - Spasojević, Ivan B
AU  - Morina, Arian
AU  - Mojović, Milos D
AU  - Stević, Zorica D
AU  - Spasić, Snezana D
AU  - Jones, David R
AU  - Spasić, Mihajlo
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1405
AB  - A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.
T2  - Redox Report
T1  - Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid
IS  - 1
VL  - 15
EP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1405
ER  - 

@article{
author = "Spasojević, Ivan B and Morina, Arian and Mojović, Milos D and Stević, Zorica D and Spasić, Snezana D and Jones, David R and Spasić, Mihajlo",
year = "2010",
abstract = "A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.",
journal = "Redox Report",
title = "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid",
number = "1",
volume = "15",
pages = "35",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1405"
}

Spasojević, I. B., Morina, A., Mojović, M. D., Stević, Z. D., Spasić, S. D., Jones, D. R.,& Spasić, M.. (2010). Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report, 15(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1405

Spasojević IB, Morina A, Mojović MD, Stević ZD, Spasić SD, Jones DR, Spasić M. Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report. 2010;15(1):null-35.
https://hdl.handle.net/21.15107/rcub_ibiss_1405 .

Spasojević, Ivan B, Morina, Arian, Mojović, Milos D, Stević, Zorica D, Spasić, Snezana D, Jones, David R, Spasić, Mihajlo, "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid" in Redox Report, 15, no. 1 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1405 .

TY  - JOUR
AU  - Kojić, Danijela K
AU  - Spasojević, Ivan B
AU  - Mojović, Milos D
AU  - Blagojević, Duško
AU  - Worland, Roger M
AU  - Grubor-Lajšić, Gordana
AU  - Spasić, Mihajlo
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1471
AB  - The aim of this study was to investigate the relationship between antioxidant enzymes and reactive oxygen species production in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae) kept at 5 degrees C, -3 degrees C and -16 degrees C for two weeks. The amount of hydrogen peroxide (H(2)O(2)), activity of antioxidant enzymes, copper zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutases (MnSOD) and catalase (CAT) in whole body homogenates, as well as the electron paramagnetic resonance (EPR) spectroscopy of this insect's whole body were analysed. A higher level of melanin radical and lower CuZnSOD and CAT activities were found in larvae kept at -3 degrees C than at 5 degrees C and -16 degrees C. At the same temperature (-3 degrees C) an elevated H(2)O(2) concentration was recorded. A possible regulatory role of H(2)O(2) at -3 degrees C, which is the temperature that triggers freezing tolerance, is suggested.
T2  - European Journal of Entomology
T1  - Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)
IS  - 3
VL  - 106
EP  - 454
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1471
ER  - 

@article{
author = "Kojić, Danijela K and Spasojević, Ivan B and Mojović, Milos D and Blagojević, Duško and Worland, Roger M and Grubor-Lajšić, Gordana and Spasić, Mihajlo",
year = "2009",
abstract = "The aim of this study was to investigate the relationship between antioxidant enzymes and reactive oxygen species production in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae) kept at 5 degrees C, -3 degrees C and -16 degrees C for two weeks. The amount of hydrogen peroxide (H(2)O(2)), activity of antioxidant enzymes, copper zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutases (MnSOD) and catalase (CAT) in whole body homogenates, as well as the electron paramagnetic resonance (EPR) spectroscopy of this insect's whole body were analysed. A higher level of melanin radical and lower CuZnSOD and CAT activities were found in larvae kept at -3 degrees C than at 5 degrees C and -16 degrees C. At the same temperature (-3 degrees C) an elevated H(2)O(2) concentration was recorded. A possible regulatory role of H(2)O(2) at -3 degrees C, which is the temperature that triggers freezing tolerance, is suggested.",
journal = "European Journal of Entomology",
title = "Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)",
number = "3",
volume = "106",
pages = "454",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1471"
}

Kojić, D. K., Spasojević, I. B., Mojović, M. D., Blagojević, D., Worland, R. M., Grubor-Lajšić, G.,& Spasić, M.. (2009). Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae). in European Journal of Entomology, 106(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1471

Kojić DK, Spasojević IB, Mojović MD, Blagojević D, Worland RM, Grubor-Lajšić G, Spasić M. Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae). in European Journal of Entomology. 2009;106(3):null-454.
https://hdl.handle.net/21.15107/rcub_ibiss_1471 .

Kojić, Danijela K, Spasojević, Ivan B, Mojović, Milos D, Blagojević, Duško, Worland, Roger M, Grubor-Lajšić, Gordana, Spasić, Mihajlo, "Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)" in European Journal of Entomology, 106, no. 3 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1471 .

TY  - CONF
AU  - Mojović, Milos D
AU  - Spasojević, Ivan B
AU  - Spasić, Mihajlo
AU  - Bacić, Goran G
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1665
C3  - Free Radical Research
T1  - Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems
IS  - null
VL  - 40
EP  - S72
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1665
ER  - 

@conference{
author = "Mojović, Milos D and Spasojević, Ivan B and Spasić, Mihajlo and Bacić, Goran G",
year = "2006",
journal = "Free Radical Research",
title = "Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems",
number = "null",
volume = "40",
pages = "S72",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1665"
}

Mojović, M. D., Spasojević, I. B., Spasić, M.,& Bacić, G. G.. (2006). Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems. in Free Radical Research, 40(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1665

Mojović MD, Spasojević IB, Spasić M, Bacić GG. Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems. in Free Radical Research. 2006;40(null):null-S72.
https://hdl.handle.net/21.15107/rcub_ibiss_1665 .

Mojović, Milos D, Spasojević, Ivan B, Spasić, Mihajlo, Bacić, Goran G, "Fenton reaction produces hydrogen radical (center dot H) in chemical and biological systems" in Free Radical Research, 40, no. null (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1665 .

TY  - CONF
AU  - Spasojević, Ivan B
AU  - Mojović, Milos D
AU  - Stević, Zorica D
AU  - Batas, Valentina
AU  - Bacić, Goran G
AU  - Spasić, Mihajlo
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1666
C3  - Free Radical Research
T1  - Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS
IS  - null
VL  - 40
EP  - S90
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1666
ER  - 

@conference{
author = "Spasojević, Ivan B and Mojović, Milos D and Stević, Zorica D and Batas, Valentina and Bacić, Goran G and Spasić, Mihajlo",
year = "2006",
journal = "Free Radical Research",
title = "Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS",
number = "null",
volume = "40",
pages = "S90",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1666"
}

Spasojević, I. B., Mojović, M. D., Stević, Z. D., Batas, V., Bacić, G. G.,& Spasić, M.. (2006). Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS. in Free Radical Research, 40(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1666

Spasojević IB, Mojović MD, Stević ZD, Batas V, Bacić GG, Spasić M. Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS. in Free Radical Research. 2006;40(null):null-S90.
https://hdl.handle.net/21.15107/rcub_ibiss_1666 .

Spasojević, Ivan B, Mojović, Milos D, Stević, Zorica D, Batas, Valentina, Bacić, Goran G, Spasić, Mihajlo, "Capacity of cerebrospinal fluid to transform hydrogen peroxide-relation to neurodegenerative changes in ALS" in Free Radical Research, 40, no. null (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1666 .