TY  - CONF
AU  - Nikolić, Biljana
AU  - Marinković, Jelena
AU  - Marković, Tatjana
AU  - Radulović, Milena
AU  - Mitić Ćulafić, Dragana
AU  - Đukanović, Stefana
AU  - Ćirić, Ana
AU  - Tasić, Gvozden
AU  - Bošković, Marko
AU  - Marković, Dejan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4376
AB  - The interest in natural products applications as antimicrobial agents is rapidly growing, mainly due to 
increasing microbial resistance to conventional therapeutics. Essential oils (EOs) seem to be especially 
attractive, since many of them possess high antimicrobial potential and none revealed resistance issue. EOs 
have already found applications in endodontic practice, mainly as sealers, but their antimicrobial potential 
has not been explored in this field yet. Taking this into account, the objective of this study was to investigate 
the antibacterial and antibiofilm efficacy of Cymbopogon martinii EO against bacterial strains relevant for 
endodontic therapy. Chemical composition of EO was determined by GC-MS/FID analysis. Antibacterial 
potential was screened in microdilution assay, detecting minimal inhibitory and bactericidal concentrations 
(MICs and MBCs) and performed on Enterococcus faecalis, Streptococcus mitis and Streptococcus 
sanguinis. Eradicating potential against biofilms preformed of each bacterial species as well as of all three 
bacteria, was determined in vitro in crystal violet (CV) assay. For screening of in situ effect against biofilm 
preformed in root canals of extracted teeth, the EO-based irrigant was formulated. Its antibiofilm potential 
was evaluated by plate counting (PC) assay, performed with single E. faecalis biofilm and multispecies 
biofilm composed of all three strains. Single E. faecalis biofilm was selected since it is the most relevant 
intracanal pathogen, while multispecies biofilm was screened to better mimic clinical conditions.
Chemical analysis revealed that geraniol was the most abundant constituent (83.5%), followed by geranyl 
acetate (8.4%). Determined MIC and MBC values were the lowest for E. faecalis (0.12 and 0.25 mg mL-1
, 
respectively), and the highest for S. sanguinis (2.95 and 5.90 mg mL-1
, respectively). CV assay pointed out 
that EO successfully eradicated in vitro preformed biofilms of E. faecalis and S. mitis (approximately 30% 
reduction), but had no effect on S. sanguinis and multispecies biofilms. EO-based irrigant used for 
determination of in situ antibiofilm potential was pre-screened for its physicochemical characteristics: a 
mean particle diameter and polydispersity index was 101 ± 3 nm and 0.36 ± 0.06, respectively. PC assay 
showed that eradicating potential of applied irrigant was weak against both E. faecalis and multispecies 
intracanal biofilm (reduction of logCFU was 0.76 and 0.52, respectively). However, successive irrigation 
with conventional dental irrigants (sodium hypochlorite and sterile saline), followed by C. martini EO-based 
irrigant, notably increased the biofilm reduction (1.89 and 1.69, respectively).
In conclusion, C. martini EO possesses notable antibacterial potential that could be further investigated for 
different dental applications.
PB  - Bragança, Portugal: Instituto Politécnico de Bragança (IPB)
C3  - 1st Natural products application: Health, Cosmetic and Food: book of abstracts
T1  - Antimicrobial potential of Cymbopogon martinii essential oil and dental irrigant based on it against root canal biofilms
SP  - 109
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4376
ER  - 

@conference{
author = "Nikolić, Biljana and Marinković, Jelena and Marković, Tatjana and Radulović, Milena and Mitić Ćulafić, Dragana and Đukanović, Stefana and Ćirić, Ana and Tasić, Gvozden and Bošković, Marko and Marković, Dejan",
year = "2021",
abstract = "The interest in natural products applications as antimicrobial agents is rapidly growing, mainly due to 
increasing microbial resistance to conventional therapeutics. Essential oils (EOs) seem to be especially 
attractive, since many of them possess high antimicrobial potential and none revealed resistance issue. EOs 
have already found applications in endodontic practice, mainly as sealers, but their antimicrobial potential 
has not been explored in this field yet. Taking this into account, the objective of this study was to investigate 
the antibacterial and antibiofilm efficacy of Cymbopogon martinii EO against bacterial strains relevant for 
endodontic therapy. Chemical composition of EO was determined by GC-MS/FID analysis. Antibacterial 
potential was screened in microdilution assay, detecting minimal inhibitory and bactericidal concentrations 
(MICs and MBCs) and performed on Enterococcus faecalis, Streptococcus mitis and Streptococcus 
sanguinis. Eradicating potential against biofilms preformed of each bacterial species as well as of all three 
bacteria, was determined in vitro in crystal violet (CV) assay. For screening of in situ effect against biofilm 
preformed in root canals of extracted teeth, the EO-based irrigant was formulated. Its antibiofilm potential 
was evaluated by plate counting (PC) assay, performed with single E. faecalis biofilm and multispecies 
biofilm composed of all three strains. Single E. faecalis biofilm was selected since it is the most relevant 
intracanal pathogen, while multispecies biofilm was screened to better mimic clinical conditions.
Chemical analysis revealed that geraniol was the most abundant constituent (83.5%), followed by geranyl 
acetate (8.4%). Determined MIC and MBC values were the lowest for E. faecalis (0.12 and 0.25 mg mL-1
, 
respectively), and the highest for S. sanguinis (2.95 and 5.90 mg mL-1
, respectively). CV assay pointed out 
that EO successfully eradicated in vitro preformed biofilms of E. faecalis and S. mitis (approximately 30% 
reduction), but had no effect on S. sanguinis and multispecies biofilms. EO-based irrigant used for 
determination of in situ antibiofilm potential was pre-screened for its physicochemical characteristics: a 
mean particle diameter and polydispersity index was 101 ± 3 nm and 0.36 ± 0.06, respectively. PC assay 
showed that eradicating potential of applied irrigant was weak against both E. faecalis and multispecies 
intracanal biofilm (reduction of logCFU was 0.76 and 0.52, respectively). However, successive irrigation 
with conventional dental irrigants (sodium hypochlorite and sterile saline), followed by C. martini EO-based 
irrigant, notably increased the biofilm reduction (1.89 and 1.69, respectively).
In conclusion, C. martini EO possesses notable antibacterial potential that could be further investigated for 
different dental applications.",
publisher = "Bragança, Portugal: Instituto Politécnico de Bragança (IPB)",
journal = "1st Natural products application: Health, Cosmetic and Food: book of abstracts",
title = "Antimicrobial potential of Cymbopogon martinii essential oil and dental irrigant based on it against root canal biofilms",
pages = "109",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4376"
}

Nikolić, B., Marinković, J., Marković, T., Radulović, M., Mitić Ćulafić, D., Đukanović, S., Ćirić, A., Tasić, G., Bošković, M.,& Marković, D.. (2021). Antimicrobial potential of Cymbopogon martinii essential oil and dental irrigant based on it against root canal biofilms. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts
Bragança, Portugal: Instituto Politécnico de Bragança (IPB)., 109.
https://hdl.handle.net/21.15107/rcub_ibiss_4376

Nikolić B, Marinković J, Marković T, Radulović M, Mitić Ćulafić D, Đukanović S, Ćirić A, Tasić G, Bošković M, Marković D. Antimicrobial potential of Cymbopogon martinii essential oil and dental irrigant based on it against root canal biofilms. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts. 2021;:109.
https://hdl.handle.net/21.15107/rcub_ibiss_4376 .

Nikolić, Biljana, Marinković, Jelena, Marković, Tatjana, Radulović, Milena, Mitić Ćulafić, Dragana, Đukanović, Stefana, Ćirić, Ana, Tasić, Gvozden, Bošković, Marko, Marković, Dejan, "Antimicrobial potential of Cymbopogon martinii essential oil and dental irrigant based on it against root canal biofilms" in 1st Natural products application: Health, Cosmetic and Food: book of abstracts (2021):109,
https://hdl.handle.net/21.15107/rcub_ibiss_4376 .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Mitić Ćulafić, Dragana
AU  - Nikolić, Biljana
AU  - Đukanović, Stefana
AU  - Marković, Tatjana
AU  - Tasić, Gvozden
AU  - Ćirić, Ana
AU  - Marković, Dejan
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32707220
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3831
AB  - OBJECTIVE The objective was to determine the antibiofilm efficacy of Cymbopogon martinii and Thymus zygis essential oils and to estimate the disruption potential of oil-based endodontic irrigants on the multispecies biofilm formed in the root canals of extracted teeth. DESIGN The essential oils were characterized (GC-MS), while their antibacterial and cytotoxic properties were detected by microdilution and MTT assays. Particles sizes and polydispersity indices were determined for the irrigants. The isolates from root canals of pediatric patients were identified (MALDI-TOF). The multispecies biofilms were formed from Streptococcus mitis, Streptococcus sanguinis and Enterococcus faecalis. Antibiofilm properties of the essential oils and irrigants were determined by crystal violet and plate counting assay. Statistical analysis was performed by one-way ANOVA. RESULTS The screening of biofilm biomass revealed a disruption potential of C. martinii essential oil. The plate counting assay showed the efficacy of both oils in diminishing cell viability: high in biofilms (reduction of log10CFU was 2.75-2.87) and moderate in the planktons formed above. The essential oil-based irrigants showed the same antibiofilm activity as in the control (1.5 % sodium hypochlorite) for C. martini and almost 2-fold higher for T. zygis. Successive irrigations with 1.5 % sodium hypochlorite, saline and an oil-based irrigant was more efficient for C. martini than for the control (reductions of log10CFU was 1.69 and 1.14, respectively). CONCLUSIONS Notable activities achieved by essential oils and oil-based irrigants, particularly regarding C. martini, against the biofilm consisting of S. mitis, S. sanguinis, and E. faecalis, are expected to encourage further research in endodontics.
PB  - Elsevier Ltd
T2  - Archives of Oral Biology
T1  - Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.
VL  - 117
DO  - 10.1016/j.archoralbio.2020.104842
SP  - 104842
ER  - 

@article{
author = "Marinković, Jelena and Mitić Ćulafić, Dragana and Nikolić, Biljana and Đukanović, Stefana and Marković, Tatjana and Tasić, Gvozden and Ćirić, Ana and Marković, Dejan",
year = "2020",
abstract = "OBJECTIVE The objective was to determine the antibiofilm efficacy of Cymbopogon martinii and Thymus zygis essential oils and to estimate the disruption potential of oil-based endodontic irrigants on the multispecies biofilm formed in the root canals of extracted teeth. DESIGN The essential oils were characterized (GC-MS), while their antibacterial and cytotoxic properties were detected by microdilution and MTT assays. Particles sizes and polydispersity indices were determined for the irrigants. The isolates from root canals of pediatric patients were identified (MALDI-TOF). The multispecies biofilms were formed from Streptococcus mitis, Streptococcus sanguinis and Enterococcus faecalis. Antibiofilm properties of the essential oils and irrigants were determined by crystal violet and plate counting assay. Statistical analysis was performed by one-way ANOVA. RESULTS The screening of biofilm biomass revealed a disruption potential of C. martinii essential oil. The plate counting assay showed the efficacy of both oils in diminishing cell viability: high in biofilms (reduction of log10CFU was 2.75-2.87) and moderate in the planktons formed above. The essential oil-based irrigants showed the same antibiofilm activity as in the control (1.5 % sodium hypochlorite) for C. martini and almost 2-fold higher for T. zygis. Successive irrigations with 1.5 % sodium hypochlorite, saline and an oil-based irrigant was more efficient for C. martini than for the control (reductions of log10CFU was 1.69 and 1.14, respectively). CONCLUSIONS Notable activities achieved by essential oils and oil-based irrigants, particularly regarding C. martini, against the biofilm consisting of S. mitis, S. sanguinis, and E. faecalis, are expected to encourage further research in endodontics.",
publisher = "Elsevier Ltd",
journal = "Archives of Oral Biology",
title = "Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.",
volume = "117",
doi = "10.1016/j.archoralbio.2020.104842",
pages = "104842"
}

Marinković, J., Mitić Ćulafić, D., Nikolić, B., Đukanović, S., Marković, T., Tasić, G., Ćirić, A.,& Marković, D.. (2020). Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.. in Archives of Oral Biology
Elsevier Ltd., 117, 104842.
https://doi.org/10.1016/j.archoralbio.2020.104842

Marinković J, Mitić Ćulafić D, Nikolić B, Đukanović S, Marković T, Tasić G, Ćirić A, Marković D. Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.. in Archives of Oral Biology. 2020;117:104842.
doi:10.1016/j.archoralbio.2020.104842 .

Marinković, Jelena, Mitić Ćulafić, Dragana, Nikolić, Biljana, Đukanović, Stefana, Marković, Tatjana, Tasić, Gvozden, Ćirić, Ana, Marković, Dejan, "Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals." in Archives of Oral Biology, 117 (2020):104842,
https://doi.org/10.1016/j.archoralbio.2020.104842 . .

TY  - JOUR
AU  - Cvetković, Stefana
AU  - Todorović, Slađana
AU  - Nastasijević, Branislav
AU  - Mitić-Ćulafić, Dragana
AU  - Đukanović, Stefana
AU  - Knežević-Vukčević, Jelena
AU  - Nikolić, Biljana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3712
AB  - Medicinal plant Gentiana lutea is considered as an official drug for gastrointestinal disorders in many pharmacopoeias. Its uncontrolled overexploitation led to the protection regime and encouraged development of in vitro culture. The aim of this work was to chemically characterize root and leaf methanol extracts of plant grown in plantation and in vitro conditions, and to determine their antigenotoxicity against heterocyclic aromatic amines IQ and PhIP, the food-borne mutagens that are formed during high temperature cooking of protein-reach foods. High yields and growth ratios were determined for in vitro plants. Higher content of polyphenols and flavonoids were determined in aerial parts, while UPLC-MS/MS analysis pointed at the richness of in vitro grown shoot extract (GLvS) in active constituents, and high amounts of gentiopicroside in all tested extracts. Notable amounts of sweroside were detected in plantation root extract (GLR) and in GLvS, and of loganic acid in plantation leaf extract (GLL) and GLvS. Two experimental models for genotoxicity/antigenotoxicity study, i.e. SOS/umuC test with metabolic activation (addition of rat liver S9 fraction) and alkaline comet assay on hepatocellur carcinoma HepG2 cells indicated dual effects: genotoxic of high and antigenotoxic of lower non-genotoxic doses. While in vitro extracts were genotoxic in both models, plantation extracts were active only in SOS/umuC with S9. In contrast, inhibition of genotoxicity was observed in both models for all extracts (up to 83 %). Results of DPPH assay indicated that antioxidativity could be responsible for antigenotoxic properties. In conclusion, genoprotective effect encourages further investigation of antigenotoxicity, while high growth ratio and richness of GLvS in active compounds justify the use of in vitro cultivation method.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Assessment of genoprotective effects of Gentiana lutea extracts prepared from plants grown in field and in vitro
VL  - 154
DO  - 10.1016/j.indcrop.2020.112690
SP  - 112690
ER  - 

@article{
author = "Cvetković, Stefana and Todorović, Slađana and Nastasijević, Branislav and Mitić-Ćulafić, Dragana and Đukanović, Stefana and Knežević-Vukčević, Jelena and Nikolić, Biljana",
year = "2020",
abstract = "Medicinal plant Gentiana lutea is considered as an official drug for gastrointestinal disorders in many pharmacopoeias. Its uncontrolled overexploitation led to the protection regime and encouraged development of in vitro culture. The aim of this work was to chemically characterize root and leaf methanol extracts of plant grown in plantation and in vitro conditions, and to determine their antigenotoxicity against heterocyclic aromatic amines IQ and PhIP, the food-borne mutagens that are formed during high temperature cooking of protein-reach foods. High yields and growth ratios were determined for in vitro plants. Higher content of polyphenols and flavonoids were determined in aerial parts, while UPLC-MS/MS analysis pointed at the richness of in vitro grown shoot extract (GLvS) in active constituents, and high amounts of gentiopicroside in all tested extracts. Notable amounts of sweroside were detected in plantation root extract (GLR) and in GLvS, and of loganic acid in plantation leaf extract (GLL) and GLvS. Two experimental models for genotoxicity/antigenotoxicity study, i.e. SOS/umuC test with metabolic activation (addition of rat liver S9 fraction) and alkaline comet assay on hepatocellur carcinoma HepG2 cells indicated dual effects: genotoxic of high and antigenotoxic of lower non-genotoxic doses. While in vitro extracts were genotoxic in both models, plantation extracts were active only in SOS/umuC with S9. In contrast, inhibition of genotoxicity was observed in both models for all extracts (up to 83 %). Results of DPPH assay indicated that antioxidativity could be responsible for antigenotoxic properties. In conclusion, genoprotective effect encourages further investigation of antigenotoxicity, while high growth ratio and richness of GLvS in active compounds justify the use of in vitro cultivation method.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Assessment of genoprotective effects of Gentiana lutea extracts prepared from plants grown in field and in vitro",
volume = "154",
doi = "10.1016/j.indcrop.2020.112690",
pages = "112690"
}

Cvetković, S., Todorović, S., Nastasijević, B., Mitić-Ćulafić, D., Đukanović, S., Knežević-Vukčević, J.,& Nikolić, B.. (2020). Assessment of genoprotective effects of Gentiana lutea extracts prepared from plants grown in field and in vitro. in Industrial Crops and Products
Elsevier B.V.., 154, 112690.
https://doi.org/10.1016/j.indcrop.2020.112690

Cvetković S, Todorović S, Nastasijević B, Mitić-Ćulafić D, Đukanović S, Knežević-Vukčević J, Nikolić B. Assessment of genoprotective effects of Gentiana lutea extracts prepared from plants grown in field and in vitro. in Industrial Crops and Products. 2020;154:112690.
doi:10.1016/j.indcrop.2020.112690 .

Cvetković, Stefana, Todorović, Slađana, Nastasijević, Branislav, Mitić-Ćulafić, Dragana, Đukanović, Stefana, Knežević-Vukčević, Jelena, Nikolić, Biljana, "Assessment of genoprotective effects of Gentiana lutea extracts prepared from plants grown in field and in vitro" in Industrial Crops and Products, 154 (2020):112690,
https://doi.org/10.1016/j.indcrop.2020.112690 . .