@conference{
author = "Jović, Milena and Mladenović, Aleksandra and Milanović, Desanka and Todorović, Smilja and Prvulović, Milica and Vukojević, Anđela and Simeunović, Valentina and Sokanović, Srđan and Lončarević, Nataša",
year = "2022",
abstract = "The presence of large amounts of amyloid beta (Aβ) oligomers, amyloid plaques formation, and inflammation in the brain are one of the neuropathological characteristics
of Alzheimer's disease (AD). The use of supplements with omega3 fatty acids has been associated with reduced risk and lessened AD pathology. The purpose of this
study was to elucidate whether such a treatment could affects glial cells and macrophages behavior in the early phase of the disease.
We examined influence of fish oil (FO) treatment in 5xFAD mice, an animal model which rapidly recapitulates major hallmarks of AD amyloid pathology. Threemonth
old female 5xFAD mice received FO (100μl/animal/day) via oral gavage during 3 weeks period. Histological analysis was used to detect changes in pathological
features of AD in parietal cortex of 5xFAD mice. Aβ peptide, macrophages, microglial cells and astrocytes were detected by antiAβ42, antiIba1, antiTMEM119
and antiGFAPantibody, respectively. Immunostaining was observed by confocal microscopy. Quantification was done by ImageJ. FO supplementation alters the
behaviour of macrophages prompting them to establish a physical barrier around amyloid plaques and leads to changes in number of over all astrocytes and microglial
cells.
These results confirmed and extended previous findings suggesting that FO supplementation suppresses brain aging and has a typical pleiotropic effect, suggesting that
FO in combination with other drugs could be good approach for longterm treatment in AD suppression.",
publisher = "Federation of European Biochemical Societies",
journal = "Book of Abstracts: Joint IUBMB/FEBS Advanced Lecture Course: Molecular targets for anti-aging interventions; 2022 Sep 26 - Oct 1; Spetses Island, Greece",
title = "Short term fish oil supplementation changes glial cells function in 5XFAD mice",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5323"
}