TY  - JOUR
AU  - Nenadić, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Vesović, Nikola
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Stanković, Saša S
AU  - Ćurčić, Nina B.
AU  - Milinčić, Uroš
AU  - Petrović, Dragan
AU  - Milinčić, Miroljub
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5818
AB  - In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).
PB  - Frontiers Media S.A.
T2  - Frontiers in Ecology and Evolution
T1  - The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.
VL  - 11
DO  - 10.3389/fevo.2023.1148309
SP  - 1148309
ER  - 

@article{
author = "Nenadić, Marija and Stojković, Dejan and Soković, Marina and Ćirić, Ana and Dimkić, Ivica and Janakiev, Tamara and Vesović, Nikola and Vujisić, Ljubodrag and Todosijević, Marina and Stanković, Saša S and Ćurčić, Nina B. and Milinčić, Uroš and Petrović, Dragan and Milinčić, Miroljub and Ćurčić, Srećko",
year = "2023",
abstract = "In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Ecology and Evolution",
title = "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.",
volume = "11",
doi = "10.3389/fevo.2023.1148309",
pages = "1148309"
}

Nenadić, M., Stojković, D., Soković, M., Ćirić, A., Dimkić, I., Janakiev, T., Vesović, N., Vujisić, L., Todosijević, M., Stanković, S. S., Ćurčić, N. B., Milinčić, U., Petrović, D., Milinčić, M.,& Ćurčić, S.. (2023). The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution
Frontiers Media S.A.., 11, 1148309.
https://doi.org/10.3389/fevo.2023.1148309

Nenadić M, Stojković D, Soković M, Ćirić A, Dimkić I, Janakiev T, Vesović N, Vujisić L, Todosijević M, Stanković SS, Ćurčić NB, Milinčić U, Petrović D, Milinčić M, Ćurčić S. The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution. 2023;11:1148309.
doi:10.3389/fevo.2023.1148309 .

Nenadić, Marija, Stojković, Dejan, Soković, Marina, Ćirić, Ana, Dimkić, Ivica, Janakiev, Tamara, Vesović, Nikola, Vujisić, Ljubodrag, Todosijević, Marina, Stanković, Saša S, Ćurčić, Nina B., Milinčić, Uroš, Petrović, Dragan, Milinčić, Miroljub, Ćurčić, Srećko, "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities." in Frontiers in Ecology and Evolution, 11 (2023):1148309,
https://doi.org/10.3389/fevo.2023.1148309 . .

TY  - JOUR
AU  - Dimkić, Ivica
AU  - Fira, Đorđe
AU  - Janakiev, Tamara
AU  - Kabić, Jovana
AU  - Stupar, Miloš
AU  - Nenadić, Marija
AU  - Unković, Nikola
AU  - Ljaljević Grbić, Milica
PY  - 2021
UR  - internal-pdf://Dimkić et al. - 2021 - The microbiome of bat guano for what is this knowledge important.pdf
UR  - https://doi.org/10.1007/s00253-021-11143-y
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4140
AB  - Abstract: Bats as flying mammals are potent vectors and natural reservoir hosts for many infectious viruses, bacteria, and fungi, also detected in their excreta such as guano. Accelerated deforestation, urbanization, and anthropization hastily lead to overpopulation of the bats in urban areas allowing easy interaction with other animals, expansion, and emergence of new zoonotic disease outbreaks potentially harmful to humans. Therefore, getting new insights in the microbiome of bat guano from different places represents an imperative for the future. Furthermore, the use of novel high-throughput sequencing technologies allows better insight in guano microbiome and potentially indicated that some species could be typical guano-dwelling members. Bats are well known as a natural reservoir of many zoonotic viruses such as Ebola, Nipah, Marburg, lyssaviruses, rabies, henipaviruses, and many coronaviruses which caused a high number of outbreaks including ongoing COVID-19 pandemic. Additionally, many bacterial and fungal pathogens were identified as common guano residents. Thus, the presence of multi-drug-resistant bacteria as environmental reservoirs of extended spectrum β-lactamases and carbapenemase-producing strains has been confirmed. Bat guano is the most suitable substrate for fungal reproduction and dissemination, including pathogenic yeasts and keratinophilic and dimorphic human pathogenic fungi known as notorious causative agents of severe endemic mycoses like histoplasmosis and fatal cryptococcosis, especially deadly in immunocompromised individuals. This review provides an overview of bat guano microbiota diversity and the significance of autochthonous and pathogenic taxa for humans and the environment, highlighting better understanding in preventing emerging diseases. Key points: Bat guano as reservoir and source for spreading of autochthonous and pathogenic microbiotaBat guano vs. novel zoonotic disease outbreaksDestruction of bat natural habitats urgently demands increased human awareness.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Applied Microbiology and Biotechnology
T1  - The microbiome of bat guano: for what is this knowledge important?
IS  - 4
VL  - 105
DO  - 10.1007/s00253-021-11143-y
SP  - 1407
EP  - 1419
ER  - 

@article{
author = "Dimkić, Ivica and Fira, Đorđe and Janakiev, Tamara and Kabić, Jovana and Stupar, Miloš and Nenadić, Marija and Unković, Nikola and Ljaljević Grbić, Milica",
year = "2021",
abstract = "Abstract: Bats as flying mammals are potent vectors and natural reservoir hosts for many infectious viruses, bacteria, and fungi, also detected in their excreta such as guano. Accelerated deforestation, urbanization, and anthropization hastily lead to overpopulation of the bats in urban areas allowing easy interaction with other animals, expansion, and emergence of new zoonotic disease outbreaks potentially harmful to humans. Therefore, getting new insights in the microbiome of bat guano from different places represents an imperative for the future. Furthermore, the use of novel high-throughput sequencing technologies allows better insight in guano microbiome and potentially indicated that some species could be typical guano-dwelling members. Bats are well known as a natural reservoir of many zoonotic viruses such as Ebola, Nipah, Marburg, lyssaviruses, rabies, henipaviruses, and many coronaviruses which caused a high number of outbreaks including ongoing COVID-19 pandemic. Additionally, many bacterial and fungal pathogens were identified as common guano residents. Thus, the presence of multi-drug-resistant bacteria as environmental reservoirs of extended spectrum β-lactamases and carbapenemase-producing strains has been confirmed. Bat guano is the most suitable substrate for fungal reproduction and dissemination, including pathogenic yeasts and keratinophilic and dimorphic human pathogenic fungi known as notorious causative agents of severe endemic mycoses like histoplasmosis and fatal cryptococcosis, especially deadly in immunocompromised individuals. This review provides an overview of bat guano microbiota diversity and the significance of autochthonous and pathogenic taxa for humans and the environment, highlighting better understanding in preventing emerging diseases. Key points: Bat guano as reservoir and source for spreading of autochthonous and pathogenic microbiotaBat guano vs. novel zoonotic disease outbreaksDestruction of bat natural habitats urgently demands increased human awareness.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Applied Microbiology and Biotechnology",
title = "The microbiome of bat guano: for what is this knowledge important?",
number = "4",
volume = "105",
doi = "10.1007/s00253-021-11143-y",
pages = "1407-1419"
}

Dimkić, I., Fira, Đ., Janakiev, T., Kabić, J., Stupar, M., Nenadić, M., Unković, N.,& Ljaljević Grbić, M.. (2021). The microbiome of bat guano: for what is this knowledge important?. in Applied Microbiology and Biotechnology
Springer Science and Business Media Deutschland GmbH., 105(4), 1407-1419.
https://doi.org/10.1007/s00253-021-11143-y

Dimkić I, Fira Đ, Janakiev T, Kabić J, Stupar M, Nenadić M, Unković N, Ljaljević Grbić M. The microbiome of bat guano: for what is this knowledge important?. in Applied Microbiology and Biotechnology. 2021;105(4):1407-1419.
doi:10.1007/s00253-021-11143-y .

Dimkić, Ivica, Fira, Đorđe, Janakiev, Tamara, Kabić, Jovana, Stupar, Miloš, Nenadić, Marija, Unković, Nikola, Ljaljević Grbić, Milica, "The microbiome of bat guano: for what is this knowledge important?" in Applied Microbiology and Biotechnology, 105, no. 4 (2021):1407-1419,
https://doi.org/10.1007/s00253-021-11143-y . .

TY  - JOUR
AU  - Radulović, Olga
AU  - Petrić, Marija
AU  - Raspor, Martin
AU  - Stanojević, Olja
AU  - Janakiev, Tamara
AU  - Tadić, Vojin
AU  - Stanković, Slaviša
PY  - 2019
UR  - http://www.pjoes.com/doi/10.15244/pjoes/81687
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3255
AB  - In this work, we demonstrate that the rhizosphere of common duckweed (Lemna minor) is inhabited with various phenol-resistant bacterial strains. Based on 16S rRNA sequencing, we have identified 60 rhizosphere-associated bacterial isolates belonging to 10 different bacterial genera (Pseudomonas, Hafnia, Serratia, Enterobacter, Micrococcus, Stenotrophomonas, Xanthomonas, Bacillus, Staphylococcus and Klebsiella). All isolates have been tested for phenol resistance and ability to utilize phenol as the sole carbon source. 70% of all isolates survived high doses of phenol (≥200 mg/L) and at least 27% can be potentially acclimatized by gradual increase of phenol concentration. Finally, based on high phenol resistance, ability to utilize phenol as the sole carbon source and documented low pathogenicity, we propose 5 strains as potentially excellent candidates for bioremediation. These 5 strains taxonomically correspond to Klebsiella sp., Serratia sp., and Hafnia sp., respectively. To the best of our knowledge, this is the first attempt to assess decontamination capacity of Serratia nematodiphila and Hafnia sp. in the context of bioremediation of phenol-contaminated aqueous media. Although additional analyses are needed, interaction between the common duckweed and the selected bacterial strains may be utilized in future bioremediation strategies.
T2  - Polish Journal of Environmental Studies
T1  - Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II
IS  - 2
VL  - 28
DO  - 10.15244/pjoes/81687
SP  - 811
EP  - 822
ER  - 

@article{
author = "Radulović, Olga and Petrić, Marija and Raspor, Martin and Stanojević, Olja and Janakiev, Tamara and Tadić, Vojin and Stanković, Slaviša",
year = "2019",
abstract = "In this work, we demonstrate that the rhizosphere of common duckweed (Lemna minor) is inhabited with various phenol-resistant bacterial strains. Based on 16S rRNA sequencing, we have identified 60 rhizosphere-associated bacterial isolates belonging to 10 different bacterial genera (Pseudomonas, Hafnia, Serratia, Enterobacter, Micrococcus, Stenotrophomonas, Xanthomonas, Bacillus, Staphylococcus and Klebsiella). All isolates have been tested for phenol resistance and ability to utilize phenol as the sole carbon source. 70% of all isolates survived high doses of phenol (≥200 mg/L) and at least 27% can be potentially acclimatized by gradual increase of phenol concentration. Finally, based on high phenol resistance, ability to utilize phenol as the sole carbon source and documented low pathogenicity, we propose 5 strains as potentially excellent candidates for bioremediation. These 5 strains taxonomically correspond to Klebsiella sp., Serratia sp., and Hafnia sp., respectively. To the best of our knowledge, this is the first attempt to assess decontamination capacity of Serratia nematodiphila and Hafnia sp. in the context of bioremediation of phenol-contaminated aqueous media. Although additional analyses are needed, interaction between the common duckweed and the selected bacterial strains may be utilized in future bioremediation strategies.",
journal = "Polish Journal of Environmental Studies",
title = "Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II",
number = "2",
volume = "28",
doi = "10.15244/pjoes/81687",
pages = "811-822"
}

Radulović, O., Petrić, M., Raspor, M., Stanojević, O., Janakiev, T., Tadić, V.,& Stanković, S.. (2019). Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II. in Polish Journal of Environmental Studies, 28(2), 811-822.
https://doi.org/10.15244/pjoes/81687

Radulović O, Petrić M, Raspor M, Stanojević O, Janakiev T, Tadić V, Stanković S. Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II. in Polish Journal of Environmental Studies. 2019;28(2):811-822.
doi:10.15244/pjoes/81687 .

Radulović, Olga, Petrić, Marija, Raspor, Martin, Stanojević, Olja, Janakiev, Tamara, Tadić, Vojin, Stanković, Slaviša, "Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II" in Polish Journal of Environmental Studies, 28, no. 2 (2019):811-822,
https://doi.org/10.15244/pjoes/81687 . .