TY  - CONF
AU  - Jelača, Sanja
AU  - Drača, Dijana
AU  - Dajić Stevanović, Zora
AU  - Mijatović, Sanja
AU  - Jovanović, Ivan
AU  - Jovanović, Marina
AU  - Jurišević, Marina
AU  - Arsenijević, Nebojša
AU  - Maksimović-Ivanić, Danijela
PY  - 2021
UR  - https://onlinelibrary.wiley.com/toc/15214141/2021/51/S1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4426
AB  - Alchemilla vulgaris L. has long history of usage in folk medicine especially against gynecological problems. Ethnomedicinal reports for the territory of Balkan are mentioning its well 
known biological properties against dysmenorrhea, menopausal complaints, infertility, cysts and endometriosis. Based on ethnomedicinal data on female illnesses, the objective of 
our study was to determine the effect of Alchemilla vulgaris L. ethanol extract against breast cancer cells in vitro and in vivo. Our results have showed remarkable viability decrease 
of mouse (4T1) breast cancer cells in dose‐dependent manner after the treatment with Alchemilla vulgaris L. extract. Strong inhibition of cell prolifertion was observed in treated 
cells. In parallel with this, different types of cell death was found. Certain percentage of 4T1 cells was subjected to programmed cell death‐apoptosis which was followed with caspase 
activation and confirmed by fluorescent microscopy observing tipical morphologicas features of apoptosis in treated culture. Estimation of the presence of autophagosomes shown 
that autophagy contributing  to  the cytotoxicity of the  treatment. Also, enhanced production of ROS and intracellular NO after treatment with Alchemilla vulgaris L. was  found. 
In parallel, metastatic potential of this cells is diminished. Apart from the direct effect of A. Vulgaris L. extract on tumor cells, strong potentiation of antitumor immune responce 
manifested dominantly through enhanced accumulation of activated dendritic cells and subsequently CD8+ T cells in spleen and tumor microenvironment. Above briefly described 
mode of action of Alchemilla vulgaris L. against breast cancer cells makes this plant worthwhile for further evaluation in the field of oncology.
PB  - Wiley‐VCH GmbH
C3  - 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
T1  - Antitumor potential of Alchemilla vulgaris L. in ortotopic mouse breast cancer model
IS  - Suppl 1
VL  - 51
DO  - 10.1002/eji.202170200
SP  - 351
ER  - 

@conference{
author = "Jelača, Sanja and Drača, Dijana and Dajić Stevanović, Zora and Mijatović, Sanja and Jovanović, Ivan and Jovanović, Marina and Jurišević, Marina and Arsenijević, Nebojša and Maksimović-Ivanić, Danijela",
year = "2021",
abstract = "Alchemilla vulgaris L. has long history of usage in folk medicine especially against gynecological problems. Ethnomedicinal reports for the territory of Balkan are mentioning its well 
known biological properties against dysmenorrhea, menopausal complaints, infertility, cysts and endometriosis. Based on ethnomedicinal data on female illnesses, the objective of 
our study was to determine the effect of Alchemilla vulgaris L. ethanol extract against breast cancer cells in vitro and in vivo. Our results have showed remarkable viability decrease 
of mouse (4T1) breast cancer cells in dose‐dependent manner after the treatment with Alchemilla vulgaris L. extract. Strong inhibition of cell prolifertion was observed in treated 
cells. In parallel with this, different types of cell death was found. Certain percentage of 4T1 cells was subjected to programmed cell death‐apoptosis which was followed with caspase 
activation and confirmed by fluorescent microscopy observing tipical morphologicas features of apoptosis in treated culture. Estimation of the presence of autophagosomes shown 
that autophagy contributing  to  the cytotoxicity of the  treatment. Also, enhanced production of ROS and intracellular NO after treatment with Alchemilla vulgaris L. was  found. 
In parallel, metastatic potential of this cells is diminished. Apart from the direct effect of A. Vulgaris L. extract on tumor cells, strong potentiation of antitumor immune responce 
manifested dominantly through enhanced accumulation of activated dendritic cells and subsequently CD8+ T cells in spleen and tumor microenvironment. Above briefly described 
mode of action of Alchemilla vulgaris L. against breast cancer cells makes this plant worthwhile for further evaluation in the field of oncology.",
publisher = "Wiley‐VCH GmbH",
journal = "6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting",
title = "Antitumor potential of Alchemilla vulgaris L. in ortotopic mouse breast cancer model",
number = "Suppl 1",
volume = "51",
doi = "10.1002/eji.202170200",
pages = "351"
}

Jelača, S., Drača, D., Dajić Stevanović, Z., Mijatović, S., Jovanović, I., Jovanović, M., Jurišević, M., Arsenijević, N.,& Maksimović-Ivanić, D.. (2021). Antitumor potential of Alchemilla vulgaris L. in ortotopic mouse breast cancer model. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
Wiley‐VCH GmbH., 51(Suppl 1), 351.
https://doi.org/10.1002/eji.202170200

Jelača S, Drača D, Dajić Stevanović Z, Mijatović S, Jovanović I, Jovanović M, Jurišević M, Arsenijević N, Maksimović-Ivanić D. Antitumor potential of Alchemilla vulgaris L. in ortotopic mouse breast cancer model. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting. 2021;51(Suppl 1):351.
doi:10.1002/eji.202170200 .

Jelača, Sanja, Drača, Dijana, Dajić Stevanović, Zora, Mijatović, Sanja, Jovanović, Ivan, Jovanović, Marina, Jurišević, Marina, Arsenijević, Nebojša, Maksimović-Ivanić, Danijela, "Antitumor potential of Alchemilla vulgaris L. in ortotopic mouse breast cancer model" in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting, 51, no. Suppl 1 (2021):351,
https://doi.org/10.1002/eji.202170200 . .