TY  - JOUR
AU  - Popović, Zorica
AU  - Vidaković, Vera
AU  - Mijalković, Tatjana
AU  - Krstić-Milošević, Dijana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5889
AB  - Phytochemical profiling of six natural populations of Gentianella austriaca was performed
by HPLC identification and quantification of a number of secondary metabolites, and evaluation of time series of peak areas by chemometric analysis. Phytochemical analysis of G. austriaca revealed the presence of iridoids, flavone-C-glucosides and xanthones. Twelve secondary metabolites were identified in the aerial parts, roots and seeds, including swertiamarin (SWM), gentiopicrin (GP), sweroside (SWZ), isoorientin (ISOOR), swertisin (SWE), demethylbellidifolin-8-O-glucoside (DMB-8-O-glc), bellidifolin-8-O-glucoside (BDF-8-O-glc), mangiferin (MGF), corymbiferin (CBF), corymbiferin-1-O-glucoside (CBF-1-O-glc), bellidifolin (BDF) and campestroside. Multivariate statistical analyses showed relatively low variability among populations according to secondary metabolite content. However, some pharmacologically important compounds were found in higher amounts in a few populations, which could be useful for conservation and future biotechnological procedures.
PB  - Basel: MDPI
T2  - Plants
T1  - Population-Related Variability in Qualitative and Quantitative Secondary Metabolite Profile of Gentianella austriaca (A. & J. Kern.) Holub
IS  - 13
VL  - 12
DO  - 10.3390/plants12132434
SP  - 2434
ER  - 

@article{
author = "Popović, Zorica and Vidaković, Vera and Mijalković, Tatjana and Krstić-Milošević, Dijana",
year = "2023",
abstract = "Phytochemical profiling of six natural populations of Gentianella austriaca was performed
by HPLC identification and quantification of a number of secondary metabolites, and evaluation of time series of peak areas by chemometric analysis. Phytochemical analysis of G. austriaca revealed the presence of iridoids, flavone-C-glucosides and xanthones. Twelve secondary metabolites were identified in the aerial parts, roots and seeds, including swertiamarin (SWM), gentiopicrin (GP), sweroside (SWZ), isoorientin (ISOOR), swertisin (SWE), demethylbellidifolin-8-O-glucoside (DMB-8-O-glc), bellidifolin-8-O-glucoside (BDF-8-O-glc), mangiferin (MGF), corymbiferin (CBF), corymbiferin-1-O-glucoside (CBF-1-O-glc), bellidifolin (BDF) and campestroside. Multivariate statistical analyses showed relatively low variability among populations according to secondary metabolite content. However, some pharmacologically important compounds were found in higher amounts in a few populations, which could be useful for conservation and future biotechnological procedures.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Population-Related Variability in Qualitative and Quantitative Secondary Metabolite Profile of Gentianella austriaca (A. & J. Kern.) Holub",
number = "13",
volume = "12",
doi = "10.3390/plants12132434",
pages = "2434"
}

Popović, Z., Vidaković, V., Mijalković, T.,& Krstić-Milošević, D.. (2023). Population-Related Variability in Qualitative and Quantitative Secondary Metabolite Profile of Gentianella austriaca (A. & J. Kern.) Holub. in Plants
Basel: MDPI., 12(13), 2434.
https://doi.org/10.3390/plants12132434

Popović Z, Vidaković V, Mijalković T, Krstić-Milošević D. Population-Related Variability in Qualitative and Quantitative Secondary Metabolite Profile of Gentianella austriaca (A. & J. Kern.) Holub. in Plants. 2023;12(13):2434.
doi:10.3390/plants12132434 .

Popović, Zorica, Vidaković, Vera, Mijalković, Tatjana, Krstić-Milošević, Dijana, "Population-Related Variability in Qualitative and Quantitative Secondary Metabolite Profile of Gentianella austriaca (A. & J. Kern.) Holub" in Plants, 12, no. 13 (2023):2434,
https://doi.org/10.3390/plants12132434 . .

TY  - JOUR
AU  - Banjac, Nevena
AU  - Krstić-Milošević, Dijana
AU  - Mijalković, Tatjana
AU  - Petrović, Mirjana
AU  - Ćosić, Tatjana
AU  - Stanišić, Mariana
AU  - Vinterhalter, Branka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5698
AB  - Eruca sativa is known in traditional medicine for its therapeutic effects, while young plants
are used as a salad or green food. Recently, the consumption of rocket has increased considerably,
so it has become very important for breeders. Plant tissue culture provides a platform to overcome
the problems in improving this species. In the present study, an efficient protocol for in vitro shoot
regeneration and propagation of recalcitrant rocket variety Domaća rukola was studied. Murashige
and Skoog (MS, 1962) medium containing 0.1 mg/L BA and frequent subculture over a period
of three weeks proved to be optimal for shoot multiplication with a multiplication index of over 3
and only 8.72% of hyperhydrated shoots without necrosis. Different concentrations of 2,4-D, BA, or
TDZ in combination with NAA, with or without the presence of AgNO3, were tested for de novo
shoot organogenesis (DNSO) from seedling explants. The hypocotyl explants cultured on MS with a
combination of TDZ1.0+NAA0.1+AgNO3 5.0 mg/L regenerated viable shoots with the highest rate
(25.38%) and an average number of 2.18 shoots per regenerating explant. Somatic embryogenesis
from immature zygotic embryos proved to be the best way to regenerate a recalcitrant rocket cultivar.
The highest embryogenic efficiency was achieved in explants cultured on MS medium containing
1.0 mg/L 2,4-D with a frequency of 76.64% and 5.13 mean number of regenerated somatic embryos
per explant, which were further converted into normal plants. Additionally, in vitro-produced rocket
shoots could serve as a possible promising source for the production of flavonoid kaempferol with
proven antioxidant properties.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola
IS  - 5
VL  - 9
DO  - 10.3390/horticulturae9050533
SP  - 533
ER  - 

@article{
author = "Banjac, Nevena and Krstić-Milošević, Dijana and Mijalković, Tatjana and Petrović, Mirjana and Ćosić, Tatjana and Stanišić, Mariana and Vinterhalter, Branka",
year = "2023",
abstract = "Eruca sativa is known in traditional medicine for its therapeutic effects, while young plants
are used as a salad or green food. Recently, the consumption of rocket has increased considerably,
so it has become very important for breeders. Plant tissue culture provides a platform to overcome
the problems in improving this species. In the present study, an efficient protocol for in vitro shoot
regeneration and propagation of recalcitrant rocket variety Domaća rukola was studied. Murashige
and Skoog (MS, 1962) medium containing 0.1 mg/L BA and frequent subculture over a period
of three weeks proved to be optimal for shoot multiplication with a multiplication index of over 3
and only 8.72% of hyperhydrated shoots without necrosis. Different concentrations of 2,4-D, BA, or
TDZ in combination with NAA, with or without the presence of AgNO3, were tested for de novo
shoot organogenesis (DNSO) from seedling explants. The hypocotyl explants cultured on MS with a
combination of TDZ1.0+NAA0.1+AgNO3 5.0 mg/L regenerated viable shoots with the highest rate
(25.38%) and an average number of 2.18 shoots per regenerating explant. Somatic embryogenesis
from immature zygotic embryos proved to be the best way to regenerate a recalcitrant rocket cultivar.
The highest embryogenic efficiency was achieved in explants cultured on MS medium containing
1.0 mg/L 2,4-D with a frequency of 76.64% and 5.13 mean number of regenerated somatic embryos
per explant, which were further converted into normal plants. Additionally, in vitro-produced rocket
shoots could serve as a possible promising source for the production of flavonoid kaempferol with
proven antioxidant properties.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola",
number = "5",
volume = "9",
doi = "10.3390/horticulturae9050533",
pages = "533"
}

Banjac, N., Krstić-Milošević, D., Mijalković, T., Petrović, M., Ćosić, T., Stanišić, M.,& Vinterhalter, B.. (2023). In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola. in Horticulturae
Basel: MDPI., 9(5), 533.
https://doi.org/10.3390/horticulturae9050533

Banjac N, Krstić-Milošević D, Mijalković T, Petrović M, Ćosić T, Stanišić M, Vinterhalter B. In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola. in Horticulturae. 2023;9(5):533.
doi:10.3390/horticulturae9050533 .

Banjac, Nevena, Krstić-Milošević, Dijana, Mijalković, Tatjana, Petrović, Mirjana, Ćosić, Tatjana, Stanišić, Mariana, Vinterhalter, Branka, "In Vitro Shoot Multiplication and Regeneration of the Recalcitrant Rocket (Eruca sativa Mill.) Variety Domaća Rukola" in Horticulturae, 9, no. 5 (2023):533,
https://doi.org/10.3390/horticulturae9050533 . .