Giba, Zlatko

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  • Giba, Zlatko (18)
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Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts

Božunović, Jelena; Ivanov, Marija; Petrović, Jovana; Gašić, Uroš; Nakarada, Đura; Milutinović, Milica; Aničić, Neda; Giba, Zlatko; Mišić, Danijela; Stojković, Dejan

(Basel: MDPI, 2023)

TY  - JOUR
AU  - Božunović, Jelena
AU  - Ivanov, Marija
AU  - Petrović, Jovana
AU  - Gašić, Uroš
AU  - Nakarada, Đura
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Giba, Zlatko
AU  - Mišić, Danijela
AU  - Stojković, Dejan
PY  - 2023
UR  - https://www.mdpi.com/1424-8247/16/2/245
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5490
AB  - Spiked centaury (Centaurium spicatum) is a well-known medicinal plant from the Mediterranean region with various bioactivities, but there are no studies addressing the use of different solvent systems to improve its pharmacological potential. Nine extraction procedures were adapted to study the effects of solvent composition on the content of bioactive compounds in C. spicatum extracts and on corresponding bioactivities. Targeted metabolomics was performed to obtain information on the chemical composition of extracts. Ethanol-water-based extraction procedures were the most efficient in isolating polyphenols, while less polar butanol extract contained the highest amount of iridoids. Antioxidant potential analysis revealed stronger activity in extracts with higher polyphenol content. Bacillus cereus and Staphylococus aureus were designated as the most sensitive bacterial strains to the activity of extracts, while among the micromycetes tested, Penicillium funiculosum was the most susceptible strain. Butanol extract showed antivirulence potential on Candida albicans morphological transition from yeast to hyphal form, and selected extracts were effective against biofilm formation in two Candida species. All the extracts tested in this study showed no cytotoxic activity to immortalize human skin keratinocyte cell line (HaCaT), whereas extracts obtained by ethanol-water extraction stand out for their potent wound healing effects. Moreover, the influence of the extraction solvent system on various bioactivities of C. spicatum is reported herein for the first time. Overall, the results presented in this study promote the use of C. spicatum as a source of natural products with potential antioxidant, wound healing, and antimicrobial applications that are potentially safe for human use.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts
IS  - 2
VL  - 16
DO  - 10.3390/ph16020245
SP  - 245
ER  - 
@article{
author = "Božunović, Jelena and Ivanov, Marija and Petrović, Jovana and Gašić, Uroš and Nakarada, Đura and Milutinović, Milica and Aničić, Neda and Giba, Zlatko and Mišić, Danijela and Stojković, Dejan",
year = "2023",
abstract = "Spiked centaury (Centaurium spicatum) is a well-known medicinal plant from the Mediterranean region with various bioactivities, but there are no studies addressing the use of different solvent systems to improve its pharmacological potential. Nine extraction procedures were adapted to study the effects of solvent composition on the content of bioactive compounds in C. spicatum extracts and on corresponding bioactivities. Targeted metabolomics was performed to obtain information on the chemical composition of extracts. Ethanol-water-based extraction procedures were the most efficient in isolating polyphenols, while less polar butanol extract contained the highest amount of iridoids. Antioxidant potential analysis revealed stronger activity in extracts with higher polyphenol content. Bacillus cereus and Staphylococus aureus were designated as the most sensitive bacterial strains to the activity of extracts, while among the micromycetes tested, Penicillium funiculosum was the most susceptible strain. Butanol extract showed antivirulence potential on Candida albicans morphological transition from yeast to hyphal form, and selected extracts were effective against biofilm formation in two Candida species. All the extracts tested in this study showed no cytotoxic activity to immortalize human skin keratinocyte cell line (HaCaT), whereas extracts obtained by ethanol-water extraction stand out for their potent wound healing effects. Moreover, the influence of the extraction solvent system on various bioactivities of C. spicatum is reported herein for the first time. Overall, the results presented in this study promote the use of C. spicatum as a source of natural products with potential antioxidant, wound healing, and antimicrobial applications that are potentially safe for human use.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts",
number = "2",
volume = "16",
doi = "10.3390/ph16020245",
pages = "245"
}
Božunović, J., Ivanov, M., Petrović, J., Gašić, U., Nakarada, Đ., Milutinović, M., Aničić, N., Giba, Z., Mišić, D.,& Stojković, D.. (2023). Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts. in Pharmaceuticals
Basel: MDPI., 16(2), 245.
https://doi.org/10.3390/ph16020245
Božunović J, Ivanov M, Petrović J, Gašić U, Nakarada Đ, Milutinović M, Aničić N, Giba Z, Mišić D, Stojković D. Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts. in Pharmaceuticals. 2023;16(2):245.
doi:10.3390/ph16020245 .
Božunović, Jelena, Ivanov, Marija, Petrović, Jovana, Gašić, Uroš, Nakarada, Đura, Milutinović, Milica, Aničić, Neda, Giba, Zlatko, Mišić, Danijela, Stojković, Dejan, "Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts" in Pharmaceuticals, 16, no. 2 (2023):245,
https://doi.org/10.3390/ph16020245 . .
5
2

Secoiridoid glycosides production by Centaurium maritimum (L.) Fritch hairy root cultures in temporary immersion bioreactor

Mišić, Danijela; Šiler, Branislav; Skorić, Marijana; Đuricković, Milutin S; Nestorović Živković, Jasmina; Jovanović, Vladimir; Giba, Zlatko

(2013)

TY  - JOUR
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Skorić, Marijana
AU  - Đuricković, Milutin S
AU  - Nestorović Živković, Jasmina
AU  - Jovanović, Vladimir
AU  - Giba, Zlatko
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/962
AB  - Secoiridoid glycosides are naturally occurring phytochemicals of great importance for the food and pharmaceutical industry because of their various biological activities. Certain Gentiana and Centaurium species., which are recognized as the most important sources of these compounds, have become critically endangered due to overexploitation. In this study we describe a laboratory-scale approach for further implementation in large-scale production of secoiridoid glycosides, using a hairy root culture system of Centaurium maritimum L. Fritch, an underutilized and phytochemically unexplored species. Hairy roots were induced by Agrobacterium rhizogenes strain A40M70GUS and grown in Erlenmeyer flasks, as well as in RITA temporary immersion bioreactors (TIBs). About 2-4 times higher biomass production rate and up to 8 times higher total secoiridoid glycosides production rate were achieved in RITA bioreactors. Among the selected hairy root lines, line HR3 cultured in RITA (R) TIBs proved to be the most efficient considering both biomass and secoiridoid glycosides production rate. (C) 2013 Elsevier Ltd. All rights reserved.
T2  - Process Biochemistry
T1  - Secoiridoid glycosides production by Centaurium maritimum (L.) Fritch hairy root cultures in temporary immersion bioreactor
IS  - 10
VL  - 48
SP  - 224
EP  - 1591
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_962
ER  - 
@article{
author = "Mišić, Danijela and Šiler, Branislav and Skorić, Marijana and Đuricković, Milutin S and Nestorović Živković, Jasmina and Jovanović, Vladimir and Giba, Zlatko",
year = "2013",
abstract = "Secoiridoid glycosides are naturally occurring phytochemicals of great importance for the food and pharmaceutical industry because of their various biological activities. Certain Gentiana and Centaurium species., which are recognized as the most important sources of these compounds, have become critically endangered due to overexploitation. In this study we describe a laboratory-scale approach for further implementation in large-scale production of secoiridoid glycosides, using a hairy root culture system of Centaurium maritimum L. Fritch, an underutilized and phytochemically unexplored species. Hairy roots were induced by Agrobacterium rhizogenes strain A40M70GUS and grown in Erlenmeyer flasks, as well as in RITA temporary immersion bioreactors (TIBs). About 2-4 times higher biomass production rate and up to 8 times higher total secoiridoid glycosides production rate were achieved in RITA bioreactors. Among the selected hairy root lines, line HR3 cultured in RITA (R) TIBs proved to be the most efficient considering both biomass and secoiridoid glycosides production rate. (C) 2013 Elsevier Ltd. All rights reserved.",
journal = "Process Biochemistry",
title = "Secoiridoid glycosides production by Centaurium maritimum (L.) Fritch hairy root cultures in temporary immersion bioreactor",
number = "10",
volume = "48",
pages = "224-1591",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_962"
}
Mišić, D., Šiler, B., Skorić, M., Đuricković, M. S., Nestorović Živković, J., Jovanović, V.,& Giba, Z.. (2013). Secoiridoid glycosides production by Centaurium maritimum (L.) Fritch hairy root cultures in temporary immersion bioreactor. in Process Biochemistry, 48(10), 224-1591.
https://hdl.handle.net/21.15107/rcub_ibiss_962
Mišić D, Šiler B, Skorić M, Đuricković MS, Nestorović Živković J, Jovanović V, Giba Z. Secoiridoid glycosides production by Centaurium maritimum (L.) Fritch hairy root cultures in temporary immersion bioreactor. in Process Biochemistry. 2013;48(10):224-1591.
https://hdl.handle.net/21.15107/rcub_ibiss_962 .
Mišić, Danijela, Šiler, Branislav, Skorić, Marijana, Đuricković, Milutin S, Nestorović Živković, Jasmina, Jovanović, Vladimir, Giba, Zlatko, "Secoiridoid glycosides production by Centaurium maritimum (L.) Fritch hairy root cultures in temporary immersion bioreactor" in Process Biochemistry, 48, no. 10 (2013):224-1591,
https://hdl.handle.net/21.15107/rcub_ibiss_962 .

Interaction of fire-related cues in seed germination of the potentially invasive species Paulownia tomentosa Steud

Todorović, Slađana; Božić, Dragana; Simonović, Ana; Filipović, Biljana; Dragičević, Milan B.; Giba, Zlatko; Grubišić, Dragoljub

(2010)

TY  - JOUR
AU  - Todorović, Slađana
AU  - Božić, Dragana
AU  - Simonović, Ana
AU  - Filipović, Biljana
AU  - Dragičević, Milan B.
AU  - Giba, Zlatko
AU  - Grubišić, Dragoljub
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1353
AB  - The Empress tree (Paulownia tomentosa Steud.) is a fast growing deciduous tree originating from East Asia. It is grown as an ornamental plant, but can also be used as industrial wood. However, in some parts of North America, this species is considered invasive, with high impact on autochthonous populations. We have investigated whether post-fire expansion of this species can be explained at the germination level by analyzing the interaction between physical (light and temperature) and chemical (smoke and nitrogenous compounds) fire-related cues in the regulation of P. tomentosa germination. Light is the key environmental signal in the induction of germination of positively photoblastic P. tomentosa seeds. Smoke, applied in the form of extract (liquid smoke [LS]), stimulates light-induced germination if applied during imbibition, or shortly after the inductive irradiation. This stimulatory effect of LS can be effectively prevented by far-red light, suggesting that the mechanism of smoke action is phytochrome related. The LS had no effect on the germination percentage when applied during the phase of radicle protrusion. However, LS reduced radicle elongation and slowed its protrusion. The LS had no effect on the optimal temperature range of germination (23-27 degrees C). The combined action of LS and KNO(3) in light-induced germination was more effective then when each chemical was applied separately. It can be concluded that all tested components of fire, except elevated temperature, operate to stimulate germination of P. tomentosa seeds, suggesting that the post-fire invasive potential of this species can, at least in part, be explained at the germination level.
T2  - Plant Species Biology
T1  - Interaction of fire-related cues in seed germination of the potentially invasive species Paulownia tomentosa Steud
IS  - 3
VL  - 25
EP  - 202
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1353
ER  - 
@article{
author = "Todorović, Slađana and Božić, Dragana and Simonović, Ana and Filipović, Biljana and Dragičević, Milan B. and Giba, Zlatko and Grubišić, Dragoljub",
year = "2010",
abstract = "The Empress tree (Paulownia tomentosa Steud.) is a fast growing deciduous tree originating from East Asia. It is grown as an ornamental plant, but can also be used as industrial wood. However, in some parts of North America, this species is considered invasive, with high impact on autochthonous populations. We have investigated whether post-fire expansion of this species can be explained at the germination level by analyzing the interaction between physical (light and temperature) and chemical (smoke and nitrogenous compounds) fire-related cues in the regulation of P. tomentosa germination. Light is the key environmental signal in the induction of germination of positively photoblastic P. tomentosa seeds. Smoke, applied in the form of extract (liquid smoke [LS]), stimulates light-induced germination if applied during imbibition, or shortly after the inductive irradiation. This stimulatory effect of LS can be effectively prevented by far-red light, suggesting that the mechanism of smoke action is phytochrome related. The LS had no effect on the germination percentage when applied during the phase of radicle protrusion. However, LS reduced radicle elongation and slowed its protrusion. The LS had no effect on the optimal temperature range of germination (23-27 degrees C). The combined action of LS and KNO(3) in light-induced germination was more effective then when each chemical was applied separately. It can be concluded that all tested components of fire, except elevated temperature, operate to stimulate germination of P. tomentosa seeds, suggesting that the post-fire invasive potential of this species can, at least in part, be explained at the germination level.",
journal = "Plant Species Biology",
title = "Interaction of fire-related cues in seed germination of the potentially invasive species Paulownia tomentosa Steud",
number = "3",
volume = "25",
pages = "202",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1353"
}
Todorović, S., Božić, D., Simonović, A., Filipović, B., Dragičević, M. B., Giba, Z.,& Grubišić, D.. (2010). Interaction of fire-related cues in seed germination of the potentially invasive species Paulownia tomentosa Steud. in Plant Species Biology, 25(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1353
Todorović S, Božić D, Simonović A, Filipović B, Dragičević MB, Giba Z, Grubišić D. Interaction of fire-related cues in seed germination of the potentially invasive species Paulownia tomentosa Steud. in Plant Species Biology. 2010;25(3):null-202.
https://hdl.handle.net/21.15107/rcub_ibiss_1353 .
Todorović, Slađana, Božić, Dragana, Simonović, Ana, Filipović, Biljana, Dragičević, Milan B., Giba, Zlatko, Grubišić, Dragoljub, "Interaction of fire-related cues in seed germination of the potentially invasive species Paulownia tomentosa Steud" in Plant Species Biology, 25, no. 3 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1353 .

High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds

Todorović, Slađana; Giba, Zlatko; Bacić, Goran G; Nikolić, Miroslav; Grubišić, Dragoljub

(2008)

TY  - JOUR
AU  - Todorović, Slađana
AU  - Giba, Zlatko
AU  - Bacić, Goran G
AU  - Nikolić, Miroslav
AU  - Grubišić, Dragoljub
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1498
AB  - The effect of high Mn2+ content on Centaurium pulchellum seed germination has been investigated. Seeds containing extremely high Mn2+ content were produced by culturing single-node flowering explants for 2 months in the MS-media, supplemented with Mn in concentrations ranging from 1 to 10,000 mu M. Although the seeds displayed the capacity to accumulate high amount of Mn, their germination was undisturbed. EPR spectroscopy was used to measure the ratio of free (aqueous) Mn to bound Mn and it was found that over 97% of total Mn was in the bound form. With elevating the external Mn supply, seed Mn concentration also increased, but the proportion of free Mn2+ fraction decreased from 3% in the control (1 mu M Mn) to 0.35% and 0.15% in high Mn supply (1000 mu M and 10,000 mu M, respectively). These results suggest that an elevation of internal Mn concentration in seeds is associated with increased Mn binding pools, hence Mn remains bound during germination. Consequently, the action of potentially harmful Mn2+ ions, which may generate ROS and affect seed viability, is alleviated. (C) 2008 Elsevier B.V. All rights reserved.
T2  - Environmental and Experimental Botany
T1  - High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds
IS  - 3
VL  - 64
EP  - 324
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1498
ER  - 
@article{
author = "Todorović, Slađana and Giba, Zlatko and Bacić, Goran G and Nikolić, Miroslav and Grubišić, Dragoljub",
year = "2008",
abstract = "The effect of high Mn2+ content on Centaurium pulchellum seed germination has been investigated. Seeds containing extremely high Mn2+ content were produced by culturing single-node flowering explants for 2 months in the MS-media, supplemented with Mn in concentrations ranging from 1 to 10,000 mu M. Although the seeds displayed the capacity to accumulate high amount of Mn, their germination was undisturbed. EPR spectroscopy was used to measure the ratio of free (aqueous) Mn to bound Mn and it was found that over 97% of total Mn was in the bound form. With elevating the external Mn supply, seed Mn concentration also increased, but the proportion of free Mn2+ fraction decreased from 3% in the control (1 mu M Mn) to 0.35% and 0.15% in high Mn supply (1000 mu M and 10,000 mu M, respectively). These results suggest that an elevation of internal Mn concentration in seeds is associated with increased Mn binding pools, hence Mn remains bound during germination. Consequently, the action of potentially harmful Mn2+ ions, which may generate ROS and affect seed viability, is alleviated. (C) 2008 Elsevier B.V. All rights reserved.",
journal = "Environmental and Experimental Botany",
title = "High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds",
number = "3",
volume = "64",
pages = "324",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1498"
}
Todorović, S., Giba, Z., Bacić, G. G., Nikolić, M.,& Grubišić, D.. (2008). High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds. in Environmental and Experimental Botany, 64(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1498
Todorović S, Giba Z, Bacić GG, Nikolić M, Grubišić D. High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds. in Environmental and Experimental Botany. 2008;64(3):null-324.
https://hdl.handle.net/21.15107/rcub_ibiss_1498 .
Todorović, Slađana, Giba, Zlatko, Bacić, Goran G, Nikolić, Miroslav, Grubišić, Dragoljub, "High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds" in Environmental and Experimental Botany, 64, no. 3 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1498 .

Uticaj uslova sredine tokom sazrevanja na klijanje semena mišjakinje (Stellaria media (L.) Vill.)

Jovanović, Vladan; Janjić, Vaskrsija; Nikolić, Bogdan; Stanković-Kalezić, Radmila; Ghalawnji, Nabil; Giba, Zlatko

(2008)

TY  - JOUR
AU  - Jovanović, Vladan
AU  - Janjić, Vaskrsija
AU  - Nikolić, Bogdan
AU  - Stanković-Kalezić, Radmila
AU  - Ghalawnji, Nabil
AU  - Giba, Zlatko
PY  - 2008
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/47
AB  - The timing and dynamic of seed germination of weed plants and factors affecting them make an important segment of knowledge required for planning weed control in agricultural fields. Environmental conditions existing during seed maturation are the most immediate factor affecting morphological and physiological properties of seeds. We investigated the effect of environmental conditions existing during seed maturation of chickweed (Stellaria media (L.) Vill.), a frequent and widespread weed species, on their germination in the dark and after irradiation. Seeds were sampled from chickweed plants grown inside a glasshouse and those growing wild outdoors in the vicinity of the glasshouse at 3-9 day intervals from mid-February to early June 2007. When fruits were setting in both populations simultaneously, the seeds were collected on the same dates inside and outside the glasshouse. Chickweed plants that grew outdoors began flowering and fruiting more than a month later than those growing in the glasshouse. Plants growing inside the glasshouse stopped fruiting about 20 days after those around the glasshouse. Two months after the last harvest, in August, germinability of the collected seeds was investigated. Despite the different conditions in which seeds had matured, no statistically significant difference was detected in germinability in the dark between the seeds sampled inside and outside the glasshouse, except in two of the 14 pairs of samples collected on the same dates. On the other hand, there were considerable and statistically significant differences in germinability depending on sampling dates. The lowest germination was found in samples collected both inside and outside the glasshouse in late March and early April. Irradiation of imbibing seeds with white daylight stimulated germination of all samples and significantly decreased the differences in germinability of seeds collected on different dates, while not eliminating them fully.
AB  - Poznavanje vremena i dinamike klijanja semena korovskih biljaka i faktora koji na njih utiču je u svetu važan deo korpusa znanja koja su potrebna prilikom planiranja kontrole zakorovljenosti poljoprivrednih površina. Uslovi sredine prisutni tokom sazrevanja semena su prvi faktori koji utiču na kasnije morfološke i fiziološke osobine semena. U ovom radu je ispitivan uticaj uslova sredine tokom sazrevanja semena mišjakinje (Stellaria media (L.) Vill.), česte i široko rasprostranjene korovske vrste, na klijanje u mraku i nakon osvetljavanja. Semena su brana u razmacima od tri do devet dana od sredine februara do početka juna 2007. godine sa biljaka mišjakinje gajenih u staklari i sa biljaka koje su samoniklo rasle oko staklare. U vreme kada su obe populacije plodonosile, semena su brana istog dana i ispred staklare i u staklari. Biljke mišjakinje koje su rasle oko staklare počele su sa sporadičnim cvetanjem oko 15 dana kasnije od biljaka u staklari, ali su, zbog dužeg perioda hladnog vremena koji je usledio, sa masovnijim cvetanjem i plodonošenjem počele više od mesec dana kasnije od biljaka iz staklare. Biljke u staklari su prestale da plodonose dvadesetak dana posle biljaka oko staklare. Dva meseca nakon poslednjeg branja, tokom avgusta, ispitivana je klijavost ubranih semena. I pored različitih uslova u kojima su semena sazrevala nije bilo statistički značajne razlike u klijanju u mraku semena ubranih u staklari i ubranih oko staklare, osim u dva od četrnaest parova uzoraka ubranih istog dana. S druge strane, postojale su velike, statistički značajne razlike u klijavosti u zavisnosti od datuma branja. Najnižu klijavost su imali uzorci semena ubranih krajem marta i početkom aprila i kod semena ubranih u staklari i kod onih ubranih oko staklare. Osvetljavanje imbibovanih semena belom, dnevnom svetlošću, je stimulisalo klijanje svih uzoraka i značajno umanjilo razlike u klijavosti semena ubranih različitih datuma, ali ih nije eliminisalo u potpunosti.
T2  - Acta biologica iugoslavica - serija G: Acta herbologica
T1  - Uticaj uslova sredine tokom sazrevanja na klijanje semena mišjakinje (Stellaria media (L.) Vill.)
T1  - The effect of environmental conditions during seed maturation on germination of chickweed (Stellaria media (L.) Vill.)
IS  - 1
VL  - 17
SP  - 181
EP  - 188
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_47
ER  - 
@article{
author = "Jovanović, Vladan and Janjić, Vaskrsija and Nikolić, Bogdan and Stanković-Kalezić, Radmila and Ghalawnji, Nabil and Giba, Zlatko",
year = "2008, 2008",
abstract = "The timing and dynamic of seed germination of weed plants and factors affecting them make an important segment of knowledge required for planning weed control in agricultural fields. Environmental conditions existing during seed maturation are the most immediate factor affecting morphological and physiological properties of seeds. We investigated the effect of environmental conditions existing during seed maturation of chickweed (Stellaria media (L.) Vill.), a frequent and widespread weed species, on their germination in the dark and after irradiation. Seeds were sampled from chickweed plants grown inside a glasshouse and those growing wild outdoors in the vicinity of the glasshouse at 3-9 day intervals from mid-February to early June 2007. When fruits were setting in both populations simultaneously, the seeds were collected on the same dates inside and outside the glasshouse. Chickweed plants that grew outdoors began flowering and fruiting more than a month later than those growing in the glasshouse. Plants growing inside the glasshouse stopped fruiting about 20 days after those around the glasshouse. Two months after the last harvest, in August, germinability of the collected seeds was investigated. Despite the different conditions in which seeds had matured, no statistically significant difference was detected in germinability in the dark between the seeds sampled inside and outside the glasshouse, except in two of the 14 pairs of samples collected on the same dates. On the other hand, there were considerable and statistically significant differences in germinability depending on sampling dates. The lowest germination was found in samples collected both inside and outside the glasshouse in late March and early April. Irradiation of imbibing seeds with white daylight stimulated germination of all samples and significantly decreased the differences in germinability of seeds collected on different dates, while not eliminating them fully., Poznavanje vremena i dinamike klijanja semena korovskih biljaka i faktora koji na njih utiču je u svetu važan deo korpusa znanja koja su potrebna prilikom planiranja kontrole zakorovljenosti poljoprivrednih površina. Uslovi sredine prisutni tokom sazrevanja semena su prvi faktori koji utiču na kasnije morfološke i fiziološke osobine semena. U ovom radu je ispitivan uticaj uslova sredine tokom sazrevanja semena mišjakinje (Stellaria media (L.) Vill.), česte i široko rasprostranjene korovske vrste, na klijanje u mraku i nakon osvetljavanja. Semena su brana u razmacima od tri do devet dana od sredine februara do početka juna 2007. godine sa biljaka mišjakinje gajenih u staklari i sa biljaka koje su samoniklo rasle oko staklare. U vreme kada su obe populacije plodonosile, semena su brana istog dana i ispred staklare i u staklari. Biljke mišjakinje koje su rasle oko staklare počele su sa sporadičnim cvetanjem oko 15 dana kasnije od biljaka u staklari, ali su, zbog dužeg perioda hladnog vremena koji je usledio, sa masovnijim cvetanjem i plodonošenjem počele više od mesec dana kasnije od biljaka iz staklare. Biljke u staklari su prestale da plodonose dvadesetak dana posle biljaka oko staklare. Dva meseca nakon poslednjeg branja, tokom avgusta, ispitivana je klijavost ubranih semena. I pored različitih uslova u kojima su semena sazrevala nije bilo statistički značajne razlike u klijanju u mraku semena ubranih u staklari i ubranih oko staklare, osim u dva od četrnaest parova uzoraka ubranih istog dana. S druge strane, postojale su velike, statistički značajne razlike u klijavosti u zavisnosti od datuma branja. Najnižu klijavost su imali uzorci semena ubranih krajem marta i početkom aprila i kod semena ubranih u staklari i kod onih ubranih oko staklare. Osvetljavanje imbibovanih semena belom, dnevnom svetlošću, je stimulisalo klijanje svih uzoraka i značajno umanjilo razlike u klijavosti semena ubranih različitih datuma, ali ih nije eliminisalo u potpunosti.",
journal = "Acta biologica iugoslavica - serija G: Acta herbologica",
title = "Uticaj uslova sredine tokom sazrevanja na klijanje semena mišjakinje (Stellaria media (L.) Vill.), The effect of environmental conditions during seed maturation on germination of chickweed (Stellaria media (L.) Vill.)",
number = "1",
volume = "17",
pages = "181-188",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_47"
}
Jovanović, V., Janjić, V., Nikolić, B., Stanković-Kalezić, R., Ghalawnji, N.,& Giba, Z.. (2008). Uticaj uslova sredine tokom sazrevanja na klijanje semena mišjakinje (Stellaria media (L.) Vill.). in Acta biologica iugoslavica - serija G: Acta herbologica, 17(1), 181-188.
https://hdl.handle.net/21.15107/rcub_ibiss_47
Jovanović V, Janjić V, Nikolić B, Stanković-Kalezić R, Ghalawnji N, Giba Z. Uticaj uslova sredine tokom sazrevanja na klijanje semena mišjakinje (Stellaria media (L.) Vill.). in Acta biologica iugoslavica - serija G: Acta herbologica. 2008;17(1):181-188.
https://hdl.handle.net/21.15107/rcub_ibiss_47 .
Jovanović, Vladan, Janjić, Vaskrsija, Nikolić, Bogdan, Stanković-Kalezić, Radmila, Ghalawnji, Nabil, Giba, Zlatko, "Uticaj uslova sredine tokom sazrevanja na klijanje semena mišjakinje (Stellaria media (L.) Vill.)" in Acta biologica iugoslavica - serija G: Acta herbologica, 17, no. 1 (2008):181-188,
https://hdl.handle.net/21.15107/rcub_ibiss_47 .

Basic seed germination characteristics of the endemic species Nepeta rtanjensis (Lamiaceae)

Todorović, Slađana; Živković, Suzana; Giba, Zlatko; Mišić, Danijela; Grubišić, Dragoljub

(2007)

TY  - JOUR
AU  - Todorović, Slađana
AU  - Živković, Suzana
AU  - Giba, Zlatko
AU  - Mišić, Danijela
AU  - Grubišić, Dragoljub
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1569
AB  - Nepeta rtanjensis is an endemic plant species that was first recorded in 1974 in the eastern part of Serbia. Because of the small number of discovered specimens and low seed viability, it has been difficult to determine the seed germination characteristics of this species. Using in vitro culture techniques, we obtained sufficient plants for successful reintroduction. In the first year approximately 500 000 seeds were collected and subjected to various seed germination tests. The seeds were positive photoblastic and germination was under the control of the phytochrome pigment system. The requirement for light could be substituted by gibberellins. The seeds were nitrate insensitive, but responded to treatment with liquid smoke.
T2  - Plant Species Biology
T1  - Basic seed germination characteristics of the endemic species Nepeta rtanjensis (Lamiaceae)
IS  - 3
VL  - 22
EP  - 210
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1569
ER  - 
@article{
author = "Todorović, Slađana and Živković, Suzana and Giba, Zlatko and Mišić, Danijela and Grubišić, Dragoljub",
year = "2007",
abstract = "Nepeta rtanjensis is an endemic plant species that was first recorded in 1974 in the eastern part of Serbia. Because of the small number of discovered specimens and low seed viability, it has been difficult to determine the seed germination characteristics of this species. Using in vitro culture techniques, we obtained sufficient plants for successful reintroduction. In the first year approximately 500 000 seeds were collected and subjected to various seed germination tests. The seeds were positive photoblastic and germination was under the control of the phytochrome pigment system. The requirement for light could be substituted by gibberellins. The seeds were nitrate insensitive, but responded to treatment with liquid smoke.",
journal = "Plant Species Biology",
title = "Basic seed germination characteristics of the endemic species Nepeta rtanjensis (Lamiaceae)",
number = "3",
volume = "22",
pages = "210",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1569"
}
Todorović, S., Živković, S., Giba, Z., Mišić, D.,& Grubišić, D.. (2007). Basic seed germination characteristics of the endemic species Nepeta rtanjensis (Lamiaceae). in Plant Species Biology, 22(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1569
Todorović S, Živković S, Giba Z, Mišić D, Grubišić D. Basic seed germination characteristics of the endemic species Nepeta rtanjensis (Lamiaceae). in Plant Species Biology. 2007;22(3):null-210.
https://hdl.handle.net/21.15107/rcub_ibiss_1569 .
Todorović, Slađana, Živković, Suzana, Giba, Zlatko, Mišić, Danijela, Grubišić, Dragoljub, "Basic seed germination characteristics of the endemic species Nepeta rtanjensis (Lamiaceae)" in Plant Species Biology, 22, no. 3 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1569 .

Radomir M Konjevic (1 August 1946-22 July 2006)

Grubišić, Dragoljub; Giba, Zlatko

(2007)

TY  - GEN
AU  - Grubišić, Dragoljub
AU  - Giba, Zlatko
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1608
T2  - Seed Science Research
T1  - Radomir M Konjevic (1 August 1946-22 July 2006)
IS  - 1
VL  - 17
EP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1608
ER  - 
@misc{
author = "Grubišić, Dragoljub and Giba, Zlatko",
year = "2007",
journal = "Seed Science Research",
title = "Radomir M Konjevic (1 August 1946-22 July 2006)",
number = "1",
volume = "17",
pages = "70",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1608"
}
Grubišić, D.,& Giba, Z.. (2007). Radomir M Konjevic (1 August 1946-22 July 2006). in Seed Science Research, 17(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1608
Grubišić D, Giba Z. Radomir M Konjevic (1 August 1946-22 July 2006). in Seed Science Research. 2007;17(1):null-70.
https://hdl.handle.net/21.15107/rcub_ibiss_1608 .
Grubišić, Dragoljub, Giba, Zlatko, "Radomir M Konjevic (1 August 1946-22 July 2006)" in Seed Science Research, 17, no. 1 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1608 .

Measurements of voltage-current characteristics of a plasma needle and its effect on plant cells

Puac, Nevena; Petrović, Zoran Lj; Malović, Gordana N; Đorđević, Antonije R; Živković, Suzana; Giba, Zlatko; Grubišić, Dragoljub

(2006)

TY  - JOUR
AU  - Puac, Nevena
AU  - Petrović, Zoran Lj
AU  - Malović, Gordana N
AU  - Đorđević, Antonije R
AU  - Živković, Suzana
AU  - Giba, Zlatko
AU  - Grubišić, Dragoljub
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1638
AB  - In this paper we present voltage-current-power characteristics of a plasma needle operating in the flow of helium at atmospheric pressure. In addition, we show some examples of how such a plasma affects plant tissues. In the characterization of the plasma needle, current and voltage waveforms were recorded by two derivative probes. These two probes are similar to the probes previously used by Puac et al for measuring transmitted power in low pressure CCP rf discharge. The instantaneous power was calculated from current and voltage waveforms and U-I characteristics of the discharge were determined. Regimes of operation with and without the grounding ring at the tip of the needle were considered. We have chosen two model systems to study the effect of the plasma needle on plant cells and tissues: sweet fern gametophyte (prothallus) and calli produced in vitro. Since the prothallus consists of a single layer of cells, the cytological effects could be easily examined. In addition, calli and prothallus are easy to manipulate and in vitro culture provides a possibility to work under constant and controlled conditions.
T2  - Journal of Physics D-Applied Physics
T1  - Measurements of voltage-current characteristics of a plasma needle and its effect on plant cells
IS  - 16
VL  - 39
EP  - 3519
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1638
ER  - 
@article{
author = "Puac, Nevena and Petrović, Zoran Lj and Malović, Gordana N and Đorđević, Antonije R and Živković, Suzana and Giba, Zlatko and Grubišić, Dragoljub",
year = "2006",
abstract = "In this paper we present voltage-current-power characteristics of a plasma needle operating in the flow of helium at atmospheric pressure. In addition, we show some examples of how such a plasma affects plant tissues. In the characterization of the plasma needle, current and voltage waveforms were recorded by two derivative probes. These two probes are similar to the probes previously used by Puac et al for measuring transmitted power in low pressure CCP rf discharge. The instantaneous power was calculated from current and voltage waveforms and U-I characteristics of the discharge were determined. Regimes of operation with and without the grounding ring at the tip of the needle were considered. We have chosen two model systems to study the effect of the plasma needle on plant cells and tissues: sweet fern gametophyte (prothallus) and calli produced in vitro. Since the prothallus consists of a single layer of cells, the cytological effects could be easily examined. In addition, calli and prothallus are easy to manipulate and in vitro culture provides a possibility to work under constant and controlled conditions.",
journal = "Journal of Physics D-Applied Physics",
title = "Measurements of voltage-current characteristics of a plasma needle and its effect on plant cells",
number = "16",
volume = "39",
pages = "3519",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1638"
}
Puac, N., Petrović, Z. L., Malović, G. N., Đorđević, A. R., Živković, S., Giba, Z.,& Grubišić, D.. (2006). Measurements of voltage-current characteristics of a plasma needle and its effect on plant cells. in Journal of Physics D-Applied Physics, 39(16).
https://hdl.handle.net/21.15107/rcub_ibiss_1638
Puac N, Petrović ZL, Malović GN, Đorđević AR, Živković S, Giba Z, Grubišić D. Measurements of voltage-current characteristics of a plasma needle and its effect on plant cells. in Journal of Physics D-Applied Physics. 2006;39(16):null-3519.
https://hdl.handle.net/21.15107/rcub_ibiss_1638 .
Puac, Nevena, Petrović, Zoran Lj, Malović, Gordana N, Đorđević, Antonije R, Živković, Suzana, Giba, Zlatko, Grubišić, Dragoljub, "Measurements of voltage-current characteristics of a plasma needle and its effect on plant cells" in Journal of Physics D-Applied Physics, 39, no. 16 (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1638 .

Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum

Todorović, Slađana; Grubišić, Dragoljub; Giba, Zlatko; Mišić, Danijela; Konjević, Radomir M

(2006)

TY  - JOUR
AU  - Todorović, Slađana
AU  - Grubišić, Dragoljub
AU  - Giba, Zlatko
AU  - Mišić, Danijela
AU  - Konjević, Radomir M
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1626
AB  - The effect of sucrose on fruiting, seed production, and seed germination of lesser centaury [Centaurium pulchellum (Sw.) Druce] was examined using explants of flowers and flower buds. Sucrose concentrations in the culture medium ranged from 0.003 to 0.3 M. It has been shown that the number of auxiliary buds, capsules dimension, number of viable seeds per capsule and seed dimensions increased with the increase of sucrose concentrations. The highest values were recorded at sucrose concentrations higher than 0.03 M, except for seeds size, which were larger at sucrose concentration ranging from 0.003 to 0.1 M. The germination of in vitro produced seeds was affected by previous culture history: a higher germination percentage was obtained in seeds that were raised from explants originally grown on medium with sucrose concentrations higher than 0.003 M.
T2  - Biologia Plantarum
T1  - Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum
IS  - 4
VL  - 50
EP  - 774
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1626
ER  - 
@article{
author = "Todorović, Slađana and Grubišić, Dragoljub and Giba, Zlatko and Mišić, Danijela and Konjević, Radomir M",
year = "2006",
abstract = "The effect of sucrose on fruiting, seed production, and seed germination of lesser centaury [Centaurium pulchellum (Sw.) Druce] was examined using explants of flowers and flower buds. Sucrose concentrations in the culture medium ranged from 0.003 to 0.3 M. It has been shown that the number of auxiliary buds, capsules dimension, number of viable seeds per capsule and seed dimensions increased with the increase of sucrose concentrations. The highest values were recorded at sucrose concentrations higher than 0.03 M, except for seeds size, which were larger at sucrose concentration ranging from 0.003 to 0.1 M. The germination of in vitro produced seeds was affected by previous culture history: a higher germination percentage was obtained in seeds that were raised from explants originally grown on medium with sucrose concentrations higher than 0.003 M.",
journal = "Biologia Plantarum",
title = "Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum",
number = "4",
volume = "50",
pages = "774",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1626"
}
Todorović, S., Grubišić, D., Giba, Z., Mišić, D.,& Konjević, R. M.. (2006). Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum. in Biologia Plantarum, 50(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1626
Todorović S, Grubišić D, Giba Z, Mišić D, Konjević RM. Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum. in Biologia Plantarum. 2006;50(4):null-774.
https://hdl.handle.net/21.15107/rcub_ibiss_1626 .
Todorović, Slađana, Grubišić, Dragoljub, Giba, Zlatko, Mišić, Danijela, Konjević, Radomir M, "Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum" in Biologia Plantarum, 50, no. 4 (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1626 .

Stimulation of empress tree seed germination by liquid smoke

Todorović, Slađana; Giba, Zlatko; Živković, Suzana; Grubišić, Dragoljub; Konjević, Radomir M

(2005)

TY  - JOUR
AU  - Todorović, Slađana
AU  - Giba, Zlatko
AU  - Živković, Suzana
AU  - Grubišić, Dragoljub
AU  - Konjević, Radomir M
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1688
AB  - The germination of Empress tree (Paulownia tomentosa Steud.) seeds is phytochrome-controlled. Liquid smoke could not induce germination in darkness but red light irradiation of liquid smoke imbibed seeds induced a high percentage of germination. Maximum germination was achieved at liquid smoke concentration of 0.1% (v/v) when present during the imbibition phase or during the phase of phytochrome activity. The light requirement of these seeds could be completely substituted by exogenously applied gibberellins. In the presence of liquid smoke, optimal concentrations of GA(3), GA(4), and GA(9) necessary for inducing germination were several times lower than in the controls, while that of GA(7) was equally active when applied at a concentration one order of magnitude lower. The inhibitory effect of the applied growth retardants was strongly reduced and liquid smoke, in the presence of retardants, allowed light-induced germination, if applied simultaneously or after retardants treatment.
T2  - Plant Growth Regulation
T1  - Stimulation of empress tree seed germination by liquid smoke
IS  - 2-3
VL  - 47
EP  - 148
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1688
ER  - 
@article{
author = "Todorović, Slađana and Giba, Zlatko and Živković, Suzana and Grubišić, Dragoljub and Konjević, Radomir M",
year = "2005",
abstract = "The germination of Empress tree (Paulownia tomentosa Steud.) seeds is phytochrome-controlled. Liquid smoke could not induce germination in darkness but red light irradiation of liquid smoke imbibed seeds induced a high percentage of germination. Maximum germination was achieved at liquid smoke concentration of 0.1% (v/v) when present during the imbibition phase or during the phase of phytochrome activity. The light requirement of these seeds could be completely substituted by exogenously applied gibberellins. In the presence of liquid smoke, optimal concentrations of GA(3), GA(4), and GA(9) necessary for inducing germination were several times lower than in the controls, while that of GA(7) was equally active when applied at a concentration one order of magnitude lower. The inhibitory effect of the applied growth retardants was strongly reduced and liquid smoke, in the presence of retardants, allowed light-induced germination, if applied simultaneously or after retardants treatment.",
journal = "Plant Growth Regulation",
title = "Stimulation of empress tree seed germination by liquid smoke",
number = "2-3",
volume = "47",
pages = "148",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1688"
}
Todorović, S., Giba, Z., Živković, S., Grubišić, D.,& Konjević, R. M.. (2005). Stimulation of empress tree seed germination by liquid smoke. in Plant Growth Regulation, 47(2-3).
https://hdl.handle.net/21.15107/rcub_ibiss_1688
Todorović S, Giba Z, Živković S, Grubišić D, Konjević RM. Stimulation of empress tree seed germination by liquid smoke. in Plant Growth Regulation. 2005;47(2-3):null-148.
https://hdl.handle.net/21.15107/rcub_ibiss_1688 .
Todorović, Slađana, Giba, Zlatko, Živković, Suzana, Grubišić, Dragoljub, Konjević, Radomir M, "Stimulation of empress tree seed germination by liquid smoke" in Plant Growth Regulation, 47, no. 2-3 (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1688 .

Gibberellic acid nitrite stimulates germination of two species of light-requiring seeds via the nitric oxide pathway

Jovanović, Vladimir; Giba, Zlatko; Đoković, Dejan D; Milosavljević, Slobodan M; Grubišić, Dragoljub; Konjević, Radomir M

(2005)

TY  - CONF
AU  - Jovanović, Vladimir
AU  - Giba, Zlatko
AU  - Đoković, Dejan D
AU  - Milosavljević, Slobodan M
AU  - Grubišić, Dragoljub
AU  - Konjević, Radomir M
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1716
AB  - We used two species of light-requiring seeds, Paulownia tomentosa, which have absolute light requirement (no germination in darkness), and Stellaria media seeds, which germinate in darkness to a certain extent because of presence of preformed active phytochrome, to obtain results strongly suggesting that gibberellic acid nitrite stimulates seed germination via its capability as a functional NO donor. Exogenous application of gibberellic acid nitrite stimulates gibberellin-insensitive Stellaria media seed germination in darkness as do a wide variety of NO donors. Pure gibberellic acid could replace the light requirement of P tomentosa seeds, thus enabling them to germinate in darkness. Gibberellic acid nitrite did not have this effect. A stimulative effect from gibberellic acid nitrite could be detected only after exposure of these seeds to short, 10 min, pulse of red light. Taken together, these results suggest that gibberellic activity of gibberellic acid nitrite is lost after nitrosation but, regarding to the presence of -O-NO moiety in the molecule, gibberellic acid nitrite shares stimulative properties in seed germination with other compounds with NO-releasing properties.
C3  - Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus
T1  - Gibberellic acid nitrite stimulates germination of two species of light-requiring seeds via the nitric oxide pathway
IS  - null
VL  - 1048
EP  - 481
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1716
ER  - 
@conference{
author = "Jovanović, Vladimir and Giba, Zlatko and Đoković, Dejan D and Milosavljević, Slobodan M and Grubišić, Dragoljub and Konjević, Radomir M",
year = "2005",
abstract = "We used two species of light-requiring seeds, Paulownia tomentosa, which have absolute light requirement (no germination in darkness), and Stellaria media seeds, which germinate in darkness to a certain extent because of presence of preformed active phytochrome, to obtain results strongly suggesting that gibberellic acid nitrite stimulates seed germination via its capability as a functional NO donor. Exogenous application of gibberellic acid nitrite stimulates gibberellin-insensitive Stellaria media seed germination in darkness as do a wide variety of NO donors. Pure gibberellic acid could replace the light requirement of P tomentosa seeds, thus enabling them to germinate in darkness. Gibberellic acid nitrite did not have this effect. A stimulative effect from gibberellic acid nitrite could be detected only after exposure of these seeds to short, 10 min, pulse of red light. Taken together, these results suggest that gibberellic activity of gibberellic acid nitrite is lost after nitrosation but, regarding to the presence of -O-NO moiety in the molecule, gibberellic acid nitrite shares stimulative properties in seed germination with other compounds with NO-releasing properties.",
journal = "Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus",
title = "Gibberellic acid nitrite stimulates germination of two species of light-requiring seeds via the nitric oxide pathway",
number = "null",
volume = "1048",
pages = "481",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1716"
}
Jovanović, V., Giba, Z., Đoković, D. D., Milosavljević, S. M., Grubišić, D.,& Konjević, R. M.. (2005). Gibberellic acid nitrite stimulates germination of two species of light-requiring seeds via the nitric oxide pathway. in Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus, 1048(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1716
Jovanović V, Giba Z, Đoković DD, Milosavljević SM, Grubišić D, Konjević RM. Gibberellic acid nitrite stimulates germination of two species of light-requiring seeds via the nitric oxide pathway. in Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus. 2005;1048(null):null-481.
https://hdl.handle.net/21.15107/rcub_ibiss_1716 .
Jovanović, Vladimir, Giba, Zlatko, Đoković, Dejan D, Milosavljević, Slobodan M, Grubišić, Dragoljub, Konjević, Radomir M, "Gibberellic acid nitrite stimulates germination of two species of light-requiring seeds via the nitric oxide pathway" in Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus, 1048, no. null (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1716 .

Efekat regulatora rastenja na klijanje semena kičice (Centaurium erythraea Rafn)

Mijajlović, Nada; Grubišić, Dragoljub; Giba, Zlatko; Konjević, Radomir

(2005)

TY  - JOUR
AU  - Mijajlović, Nada
AU  - Grubišić, Dragoljub
AU  - Giba, Zlatko
AU  - Konjević, Radomir
PY  - 2005
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/106
AB  - Centaury seeds are light-requiring. Long-term red light irradiation caused more than 80% of seeds to germinate. Seeds did not germinate in darkness. Gibberellic acid and GA7 can replace light, but N-substituted phtalimide AC 94,377 was ineffective. Light-induced germination was inhibited by abscisic acid and growth retardants such as ancymidol, tetcyclacis, and paclobutrazole. Growth retardant-caused inhibition can be overcome by the addition of gibberellic acid.
AB  - Klijanje semena kičice je zavisno od svetlosti. Dugotrajno osvetljavanje crvenom svetlošću dovodi do klijanja preko 80% semena. Semena ne klijaju u mraku. Giberelna kiselina i GA7 zamenjuju potrebu za svetlošću dok je N substituisani ftalimid AC 94,377 neefikasan. Klijanje indukovano svetlošću inhibiraju abscisinska kiselina i retardanti rastenja kao što su tetciklacis ancimidol i paklo-butrazol. Inhibicija klijanja izazvana retandantima može da se prevaziđe dodavanjem giberelina.
T2  - Archives of Biological Sciences
T1  - Efekat regulatora rastenja na klijanje semena kičice (Centaurium erythraea Rafn)
T1  - The effect of plant growth regulators on centaury (Centaurium erythraea Rafn) seeds germination
IS  - 1
VL  - 57
SP  - 25
EP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_106
ER  - 
@article{
author = "Mijajlović, Nada and Grubišić, Dragoljub and Giba, Zlatko and Konjević, Radomir",
year = "2005, 2005",
abstract = "Centaury seeds are light-requiring. Long-term red light irradiation caused more than 80% of seeds to germinate. Seeds did not germinate in darkness. Gibberellic acid and GA7 can replace light, but N-substituted phtalimide AC 94,377 was ineffective. Light-induced germination was inhibited by abscisic acid and growth retardants such as ancymidol, tetcyclacis, and paclobutrazole. Growth retardant-caused inhibition can be overcome by the addition of gibberellic acid., Klijanje semena kičice je zavisno od svetlosti. Dugotrajno osvetljavanje crvenom svetlošću dovodi do klijanja preko 80% semena. Semena ne klijaju u mraku. Giberelna kiselina i GA7 zamenjuju potrebu za svetlošću dok je N substituisani ftalimid AC 94,377 neefikasan. Klijanje indukovano svetlošću inhibiraju abscisinska kiselina i retardanti rastenja kao što su tetciklacis ancimidol i paklo-butrazol. Inhibicija klijanja izazvana retandantima može da se prevaziđe dodavanjem giberelina.",
journal = "Archives of Biological Sciences",
title = "Efekat regulatora rastenja na klijanje semena kičice (Centaurium erythraea Rafn), The effect of plant growth regulators on centaury (Centaurium erythraea Rafn) seeds germination",
number = "1",
volume = "57",
pages = "25-28",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_106"
}
Mijajlović, N., Grubišić, D., Giba, Z.,& Konjević, R.. (2005). Efekat regulatora rastenja na klijanje semena kičice (Centaurium erythraea Rafn). in Archives of Biological Sciences, 57(1), 25-28.
https://hdl.handle.net/21.15107/rcub_ibiss_106
Mijajlović N, Grubišić D, Giba Z, Konjević R. Efekat regulatora rastenja na klijanje semena kičice (Centaurium erythraea Rafn). in Archives of Biological Sciences. 2005;57(1):25-28.
https://hdl.handle.net/21.15107/rcub_ibiss_106 .
Mijajlović, Nada, Grubišić, Dragoljub, Giba, Zlatko, Konjević, Radomir, "Efekat regulatora rastenja na klijanje semena kičice (Centaurium erythraea Rafn)" in Archives of Biological Sciences, 57, no. 1 (2005):25-28,
https://hdl.handle.net/21.15107/rcub_ibiss_106 .

Reverzija inhibicije klijanja semena Paulownia tomentosa Steud. izazvane natrijum azidom

Živković, Suzana; Grubišić, Dragoljub; Giba, Zlatko; Konjević, Radomir

(2005)

TY  - JOUR
AU  - Živković, Suzana
AU  - Grubišić, Dragoljub
AU  - Giba, Zlatko
AU  - Konjević, Radomir
PY  - 2005
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/107
AB  - The effect of some respiratory inhibitors on light-induced Paulownia tomentosa Steud. seed germination was studied. Millimolar solution of sodium azide was sufficient to completely prevent germination induced by a 5-min red light pulse. The inhibitory effect of azide was absent if seeds were rinsed before phytochrome activation by light. Sodium azide was effective only if present in the period of Pfr activity. The escape time from azide inhibition compared to the escape from far-red light action, was delayed for about 24 hours. When azide was applied after phytochrome activation, its effect depended on how long it was present in the incubation medium. The removal of azide allowed full restoration of germination by another red light pulse and the far-red escape time did not differ from the escape of untreated, i.e. water-imbibed seeds. Potassium cyanide alone did not produce any effect in light-stimulated germination of these seeds. However, it counteracted the inhibitory effect of azide in light-stimulated germination, if applied simultaneously at a concentration three times higher.
AB  - Ispitivan je efekat nekih inhibitora disanja na klijanje svetlošću indukovanih semena Paulownia tomentosa. Potpuna inhibicija klijanja, koje je indukovano sa 5 minuta crvene svetlosti, mogla je da se postigne već milimolarnim rastvorom natrijum azida. Inhibitorni efekat azida je izostajao ako se semena isperu pre aktiviranja fitohroma osvetljavanjem. Natrijum azid je bio efektivan samo ako je prisutan u semenima u periodu aktivnosti Pfr. Vreme za koje semena izbegavaju inhibitorni efekat azida je odloženo za oko 24 časa u poređenju sa istim za inhibitorno delovanje tamno crvene svetlosti. Kada se azid odstrani, dodatni puls crvene svetlosti dovodi do potpunog obnavljanja sposobnosti za maksimalno klijanje, a vreme za koje semena izbegavaju inhibitorni efekat crvene svetlosti se ne razlikuje od istog kod netretiranih semena, tj. onih koji su inbibirala u vodi. Kalijum cijanid nema nikakav uticaj na klijanje semena indukovanih svetlošću. Međutim, ako se kalijum cijanid primeni simultano sa natrijum azidom, u tri puta većoj koncentraciji, on sprečava inhibitorni efekat natrijum azida na klijanje indukovano svetlošću.
T2  - Archives of Biological Sciences
T1  - Reverzija inhibicije klijanja semena Paulownia tomentosa Steud. izazvane natrijum azidom
T1  - The counteracting effect of potassium cyanide in sodium azide-inhibited germination of Paulownia tomentosa  Steud. seeds
IS  - 1
VL  - 57
SP  - 29
EP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_107
ER  - 
@article{
author = "Živković, Suzana and Grubišić, Dragoljub and Giba, Zlatko and Konjević, Radomir",
year = "2005, 2005",
abstract = "The effect of some respiratory inhibitors on light-induced Paulownia tomentosa Steud. seed germination was studied. Millimolar solution of sodium azide was sufficient to completely prevent germination induced by a 5-min red light pulse. The inhibitory effect of azide was absent if seeds were rinsed before phytochrome activation by light. Sodium azide was effective only if present in the period of Pfr activity. The escape time from azide inhibition compared to the escape from far-red light action, was delayed for about 24 hours. When azide was applied after phytochrome activation, its effect depended on how long it was present in the incubation medium. The removal of azide allowed full restoration of germination by another red light pulse and the far-red escape time did not differ from the escape of untreated, i.e. water-imbibed seeds. Potassium cyanide alone did not produce any effect in light-stimulated germination of these seeds. However, it counteracted the inhibitory effect of azide in light-stimulated germination, if applied simultaneously at a concentration three times higher., Ispitivan je efekat nekih inhibitora disanja na klijanje svetlošću indukovanih semena Paulownia tomentosa. Potpuna inhibicija klijanja, koje je indukovano sa 5 minuta crvene svetlosti, mogla je da se postigne već milimolarnim rastvorom natrijum azida. Inhibitorni efekat azida je izostajao ako se semena isperu pre aktiviranja fitohroma osvetljavanjem. Natrijum azid je bio efektivan samo ako je prisutan u semenima u periodu aktivnosti Pfr. Vreme za koje semena izbegavaju inhibitorni efekat azida je odloženo za oko 24 časa u poređenju sa istim za inhibitorno delovanje tamno crvene svetlosti. Kada se azid odstrani, dodatni puls crvene svetlosti dovodi do potpunog obnavljanja sposobnosti za maksimalno klijanje, a vreme za koje semena izbegavaju inhibitorni efekat crvene svetlosti se ne razlikuje od istog kod netretiranih semena, tj. onih koji su inbibirala u vodi. Kalijum cijanid nema nikakav uticaj na klijanje semena indukovanih svetlošću. Međutim, ako se kalijum cijanid primeni simultano sa natrijum azidom, u tri puta većoj koncentraciji, on sprečava inhibitorni efekat natrijum azida na klijanje indukovano svetlošću.",
journal = "Archives of Biological Sciences",
title = "Reverzija inhibicije klijanja semena Paulownia tomentosa Steud. izazvane natrijum azidom, The counteracting effect of potassium cyanide in sodium azide-inhibited germination of Paulownia tomentosa  Steud. seeds",
number = "1",
volume = "57",
pages = "29-34",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_107"
}
Živković, S., Grubišić, D., Giba, Z.,& Konjević, R.. (2005). Reverzija inhibicije klijanja semena Paulownia tomentosa Steud. izazvane natrijum azidom. in Archives of Biological Sciences, 57(1), 29-34.
https://hdl.handle.net/21.15107/rcub_ibiss_107
Živković S, Grubišić D, Giba Z, Konjević R. Reverzija inhibicije klijanja semena Paulownia tomentosa Steud. izazvane natrijum azidom. in Archives of Biological Sciences. 2005;57(1):29-34.
https://hdl.handle.net/21.15107/rcub_ibiss_107 .
Živković, Suzana, Grubišić, Dragoljub, Giba, Zlatko, Konjević, Radomir, "Reverzija inhibicije klijanja semena Paulownia tomentosa Steud. izazvane natrijum azidom" in Archives of Biological Sciences, 57, no. 1 (2005):29-34,
https://hdl.handle.net/21.15107/rcub_ibiss_107 .

The stimulatory effect of non-equilibrium (low temperature) air plasma pretreatment on light-induced germination of Paulownia tomentosa seeds

Živković, Suzana; Puac, Nevena; Giba, Zlatko; Grubišić, Dragoljub; Petrović, Zoran Lj

(2004)

TY  - JOUR
AU  - Živković, Suzana
AU  - Puac, Nevena
AU  - Giba, Zlatko
AU  - Grubišić, Dragoljub
AU  - Petrović, Zoran Lj
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1759
AB  - Light-induced germination of Paulownia tomentosa seeds was used as an experimental system to investigate the effect of pretreatments by non-equilibrium (low temperature) air plasma on the germination process. Dry P. tomentosa seeds exposed to the plasma changed their germination characteristics. Non-equilibrium plasma treatments significantly increased the light sensitivity of P. tomentosa seeds. These treatments could not substitute for the light-requirements of the seeds. After a short exposure to plasma (4 to 6 minutes) treated seeds had a germination of 75%, i.e. significantly higher than the 5-30% for seeds which did not undergo pretreatment. The results suggest that the stimulatory effect of the non-equilibrium air plasma pretreatment is not a direct photoreceptor-mediated phenomenon. Possible mechanisms of this effect are discussed.
T2  - Seed Science and Technology
T1  - The stimulatory effect of non-equilibrium (low temperature) air plasma pretreatment on light-induced germination of Paulownia tomentosa seeds
IS  - 3
VL  - 32
EP  - 701
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1759
ER  - 
@article{
author = "Živković, Suzana and Puac, Nevena and Giba, Zlatko and Grubišić, Dragoljub and Petrović, Zoran Lj",
year = "2004",
abstract = "Light-induced germination of Paulownia tomentosa seeds was used as an experimental system to investigate the effect of pretreatments by non-equilibrium (low temperature) air plasma on the germination process. Dry P. tomentosa seeds exposed to the plasma changed their germination characteristics. Non-equilibrium plasma treatments significantly increased the light sensitivity of P. tomentosa seeds. These treatments could not substitute for the light-requirements of the seeds. After a short exposure to plasma (4 to 6 minutes) treated seeds had a germination of 75%, i.e. significantly higher than the 5-30% for seeds which did not undergo pretreatment. The results suggest that the stimulatory effect of the non-equilibrium air plasma pretreatment is not a direct photoreceptor-mediated phenomenon. Possible mechanisms of this effect are discussed.",
journal = "Seed Science and Technology",
title = "The stimulatory effect of non-equilibrium (low temperature) air plasma pretreatment on light-induced germination of Paulownia tomentosa seeds",
number = "3",
volume = "32",
pages = "701",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1759"
}
Živković, S., Puac, N., Giba, Z., Grubišić, D.,& Petrović, Z. L.. (2004). The stimulatory effect of non-equilibrium (low temperature) air plasma pretreatment on light-induced germination of Paulownia tomentosa seeds. in Seed Science and Technology, 32(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1759
Živković S, Puac N, Giba Z, Grubišić D, Petrović ZL. The stimulatory effect of non-equilibrium (low temperature) air plasma pretreatment on light-induced germination of Paulownia tomentosa seeds. in Seed Science and Technology. 2004;32(3):null-701.
https://hdl.handle.net/21.15107/rcub_ibiss_1759 .
Živković, Suzana, Puac, Nevena, Giba, Zlatko, Grubišić, Dragoljub, Petrović, Zoran Lj, "The stimulatory effect of non-equilibrium (low temperature) air plasma pretreatment on light-induced germination of Paulownia tomentosa seeds" in Seed Science and Technology, 32, no. 3 (2004),
https://hdl.handle.net/21.15107/rcub_ibiss_1759 .

Nitrogen oxides as environmental sensors for seeds

Giba, Zlatko; Grubišić, Dragoljub; Konjević, Radomir M

(2003)

TY  - JOUR
AU  - Giba, Zlatko
AU  - Grubišić, Dragoljub
AU  - Konjević, Radomir M
PY  - 2003
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1763
AB  - Nitrates have long been known to promote seed germination in many species, and various proposals have been made regarding the role of nitrates in this process. One hypothesis suggests a possible ecological role for nitrate, providing the seeds with a report of soil nitrogen status and the proximity of already established plants. However, nitrogen oxides (NO, NO2), which are present in the soil, also may be information carriers that indicate not only soil nitrate content, but also microbial activity and, therefore, soil quality. Because of annual variation of soil trace gas fluxes, seeds could be provided with information on seasonal and climate changes in their surroundings. Thus, nitrogen oxides would be the outer information carriers providing the seeds with integral data about many important factors required for successful germination and seedling establishment.
T2  - Seed Science Research
T1  - Nitrogen oxides as environmental sensors for seeds
IS  - 3
VL  - 13
EP  - 196
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1763
ER  - 
@article{
author = "Giba, Zlatko and Grubišić, Dragoljub and Konjević, Radomir M",
year = "2003",
abstract = "Nitrates have long been known to promote seed germination in many species, and various proposals have been made regarding the role of nitrates in this process. One hypothesis suggests a possible ecological role for nitrate, providing the seeds with a report of soil nitrogen status and the proximity of already established plants. However, nitrogen oxides (NO, NO2), which are present in the soil, also may be information carriers that indicate not only soil nitrate content, but also microbial activity and, therefore, soil quality. Because of annual variation of soil trace gas fluxes, seeds could be provided with information on seasonal and climate changes in their surroundings. Thus, nitrogen oxides would be the outer information carriers providing the seeds with integral data about many important factors required for successful germination and seedling establishment.",
journal = "Seed Science Research",
title = "Nitrogen oxides as environmental sensors for seeds",
number = "3",
volume = "13",
pages = "196",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1763"
}
Giba, Z., Grubišić, D.,& Konjević, R. M.. (2003). Nitrogen oxides as environmental sensors for seeds. in Seed Science Research, 13(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1763
Giba Z, Grubišić D, Konjević RM. Nitrogen oxides as environmental sensors for seeds. in Seed Science Research. 2003;13(3):null-196.
https://hdl.handle.net/21.15107/rcub_ibiss_1763 .
Giba, Zlatko, Grubišić, Dragoljub, Konjević, Radomir M, "Nitrogen oxides as environmental sensors for seeds" in Seed Science Research, 13, no. 3 (2003),
https://hdl.handle.net/21.15107/rcub_ibiss_1763 .

The effects of potassium nitrate and NO-donors on phytochrome A- and phytochrome B-specific induced germination of Arabidopsis thaliana seeds

Batak, Ivan; Skorić, Marijana; Giba, Zlatko; Grubišić, Dragoljub; Poff, KL; Konjević, Radomir M

(2002)

TY  - JOUR
AU  - Batak, Ivan
AU  - Skorić, Marijana
AU  - Giba, Zlatko
AU  - Grubišić, Dragoljub
AU  - Poff, KL
AU  - Konjević, Radomir M
PY  - 2002
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1785
AB  - Nitrogenous compounds, such as potassium nitrate, potentiate germination of different species of light-requiring seeds. Using light-induced Arabidopsis thaliana seed germination as a model system, our data suggested that only phytochrome A (phyA)-specific induced germination was affected after the exogenous application of nitrates, different nitric oxide (NO)-donors (such as organic nitrates) or sodium nitroprusside. The stimulative effect was very pronounced. Treated seed samples reached maximal germination after very short periods of red-light irradiation. To a far lesser extent, these substances affected phytochrome B (phyB)-specific induced germination. In phyB-specific induced germination, potassium nitrate was most effective, but germination percentages never exceeded 50%. The least effective was sodium nitroprusside, which practically did not affect phyB-specific induced germination. These results were confirmed using corresponding phytochrome mutants.
T2  - Seed Science Research
T1  - The effects of potassium nitrate and NO-donors on phytochrome A- and phytochrome B-specific induced germination of Arabidopsis thaliana seeds
IS  - 4
VL  - 12
EP  - 259
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1785
ER  - 
@article{
author = "Batak, Ivan and Skorić, Marijana and Giba, Zlatko and Grubišić, Dragoljub and Poff, KL and Konjević, Radomir M",
year = "2002",
abstract = "Nitrogenous compounds, such as potassium nitrate, potentiate germination of different species of light-requiring seeds. Using light-induced Arabidopsis thaliana seed germination as a model system, our data suggested that only phytochrome A (phyA)-specific induced germination was affected after the exogenous application of nitrates, different nitric oxide (NO)-donors (such as organic nitrates) or sodium nitroprusside. The stimulative effect was very pronounced. Treated seed samples reached maximal germination after very short periods of red-light irradiation. To a far lesser extent, these substances affected phytochrome B (phyB)-specific induced germination. In phyB-specific induced germination, potassium nitrate was most effective, but germination percentages never exceeded 50%. The least effective was sodium nitroprusside, which practically did not affect phyB-specific induced germination. These results were confirmed using corresponding phytochrome mutants.",
journal = "Seed Science Research",
title = "The effects of potassium nitrate and NO-donors on phytochrome A- and phytochrome B-specific induced germination of Arabidopsis thaliana seeds",
number = "4",
volume = "12",
pages = "259",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1785"
}
Batak, I., Skorić, M., Giba, Z., Grubišić, D., Poff, K.,& Konjević, R. M.. (2002). The effects of potassium nitrate and NO-donors on phytochrome A- and phytochrome B-specific induced germination of Arabidopsis thaliana seeds. in Seed Science Research, 12(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1785
Batak I, Skorić M, Giba Z, Grubišić D, Poff K, Konjević RM. The effects of potassium nitrate and NO-donors on phytochrome A- and phytochrome B-specific induced germination of Arabidopsis thaliana seeds. in Seed Science Research. 2002;12(4):null-259.
https://hdl.handle.net/21.15107/rcub_ibiss_1785 .
Batak, Ivan, Skorić, Marijana, Giba, Zlatko, Grubišić, Dragoljub, Poff, KL, Konjević, Radomir M, "The effects of potassium nitrate and NO-donors on phytochrome A- and phytochrome B-specific induced germination of Arabidopsis thaliana seeds" in Seed Science Research, 12, no. 4 (2002),
https://hdl.handle.net/21.15107/rcub_ibiss_1785 .

Interaction of gibberellins and fusicoccin in growth retardant- and far red light-inhibited germination of lettuce seeds

Simonović, Ana; Grubišić, Dragan; Giba, Zlatko; Konjević, Radomir

(Springer Nature, 2000)

TY  - JOUR
AU  - Simonović, Ana
AU  - Grubišić, Dragan
AU  - Giba, Zlatko
AU  - Konjević, Radomir
PY  - 2000
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4128
AB  - Germination of lettuce seeds cv. May Queen iscompletely prevented either with 10 µM tetcyclacisor with continuous FR illumination. GA3 and the N-substituted phtalimide, AC 94,377, werepartially effective in overcoming tetcyclacis-inducedinhibition but ineffective on photoinhibited seeds. FCcompletely reversed tetcyclacis inhibition and inducedca. 60% germination in continuous FR light. Aninteraction between FC and GA3 (as well asbetween FC and AC 94,377) was evident in stimulationof germination under both inhibitory conditions.Interaction was calculated as a ratio of thepercentage of seeds germinated under the simultaneousaction of stimulators compared to their additiveeffect. This was 2.54 for tetcyclacis- and 2.95 forphotoinhibited seeds. It is concluded that thistype of interaction is promotive synergism. Themagnitude of the interaction was highest if theapplication of FC was delayed after GA3application, and the optimal time lag was 6 h fortetcyclacis-inhibited, or 24 h for photoinhibited seeds.
PB  - Springer Nature
T2  - Plant Growth Regulation
T1  - Interaction of gibberellins and fusicoccin in growth retardant- and far red light-inhibited germination of lettuce seeds
VL  - 32
DO  - 10.1023/A:1006464608469
SP  - 91
EP  - 96
ER  - 
@article{
author = "Simonović, Ana and Grubišić, Dragan and Giba, Zlatko and Konjević, Radomir",
year = "2000",
abstract = "Germination of lettuce seeds cv. May Queen iscompletely prevented either with 10 µM tetcyclacisor with continuous FR illumination. GA3 and the N-substituted phtalimide, AC 94,377, werepartially effective in overcoming tetcyclacis-inducedinhibition but ineffective on photoinhibited seeds. FCcompletely reversed tetcyclacis inhibition and inducedca. 60% germination in continuous FR light. Aninteraction between FC and GA3 (as well asbetween FC and AC 94,377) was evident in stimulationof germination under both inhibitory conditions.Interaction was calculated as a ratio of thepercentage of seeds germinated under the simultaneousaction of stimulators compared to their additiveeffect. This was 2.54 for tetcyclacis- and 2.95 forphotoinhibited seeds. It is concluded that thistype of interaction is promotive synergism. Themagnitude of the interaction was highest if theapplication of FC was delayed after GA3application, and the optimal time lag was 6 h fortetcyclacis-inhibited, or 24 h for photoinhibited seeds.",
publisher = "Springer Nature",
journal = "Plant Growth Regulation",
title = "Interaction of gibberellins and fusicoccin in growth retardant- and far red light-inhibited germination of lettuce seeds",
volume = "32",
doi = "10.1023/A:1006464608469",
pages = "91-96"
}
Simonović, A., Grubišić, D., Giba, Z.,& Konjević, R.. (2000). Interaction of gibberellins and fusicoccin in growth retardant- and far red light-inhibited germination of lettuce seeds. in Plant Growth Regulation
Springer Nature., 32, 91-96.
https://doi.org/10.1023/A:1006464608469
Simonović A, Grubišić D, Giba Z, Konjević R. Interaction of gibberellins and fusicoccin in growth retardant- and far red light-inhibited germination of lettuce seeds. in Plant Growth Regulation. 2000;32:91-96.
doi:10.1023/A:1006464608469 .
Simonović, Ana, Grubišić, Dragan, Giba, Zlatko, Konjević, Radomir, "Interaction of gibberellins and fusicoccin in growth retardant- and far red light-inhibited germination of lettuce seeds" in Plant Growth Regulation, 32 (2000):91-96,
https://doi.org/10.1023/A:1006464608469 . .
4
5

Effect of nitric oxide - releasing compounds on phytochrome - controlled germination of Empress tree seeds

Giba, Zlatko; Grubišić, Dragoljub; Todorović, S; Sajc, L; Stojaković, D; Konjević, Radomir M

(1998)

TY  - JOUR
AU  - Giba, Zlatko
AU  - Grubišić, Dragoljub
AU  - Todorović, S
AU  - Sajc, L
AU  - Stojaković, D
AU  - Konjević, Radomir M
PY  - 1998
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1852
AB  - Using different nitric oxide releasing compounds and appropriate controls we have obtained data strongly suggesting the involvement of nitric oxide in the phytochrome controlled germination of Paulownia tomentosa seeds. Direct detection of nitric oxide, under various experimental conditions, was performed by a spin-trapping technique combined with electron paramagnetic resonance (EPR) spectroscopy. The addition of methylene blue prevented light-induced and NO donors-potentiated germination of P. tomentosa seeds. This inhibition could be completely overcome by addition of gibberellin. The promotive effect of nitrite was pH dependent, maximally pronounced at the pH range where nitrite undergoes dismutation and liberates nitric oxide. Under these conditions, nitrite exerted its efficacy at the same concentrations at which nitric oxide releasing compounds such as sodium nitroprusside (SNP), S-nitroso acetylpenicillamine (SNAP), and 3-morpholinosydnonimine (SIN-1), were the most effective. Likewise, the potentiation of P. tomentosa seed germination could be achieved by chemical reduction of nitrite with Na2S2O4 during which liberation of nitric oxide could be detected.
T2  - Plant Growth Regulation
T1  - Effect of nitric oxide - releasing compounds on phytochrome - controlled germination of Empress tree seeds
IS  - 3
VL  - 26
EP  - 181
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1852
ER  - 
@article{
author = "Giba, Zlatko and Grubišić, Dragoljub and Todorović, S and Sajc, L and Stojaković, D and Konjević, Radomir M",
year = "1998",
abstract = "Using different nitric oxide releasing compounds and appropriate controls we have obtained data strongly suggesting the involvement of nitric oxide in the phytochrome controlled germination of Paulownia tomentosa seeds. Direct detection of nitric oxide, under various experimental conditions, was performed by a spin-trapping technique combined with electron paramagnetic resonance (EPR) spectroscopy. The addition of methylene blue prevented light-induced and NO donors-potentiated germination of P. tomentosa seeds. This inhibition could be completely overcome by addition of gibberellin. The promotive effect of nitrite was pH dependent, maximally pronounced at the pH range where nitrite undergoes dismutation and liberates nitric oxide. Under these conditions, nitrite exerted its efficacy at the same concentrations at which nitric oxide releasing compounds such as sodium nitroprusside (SNP), S-nitroso acetylpenicillamine (SNAP), and 3-morpholinosydnonimine (SIN-1), were the most effective. Likewise, the potentiation of P. tomentosa seed germination could be achieved by chemical reduction of nitrite with Na2S2O4 during which liberation of nitric oxide could be detected.",
journal = "Plant Growth Regulation",
title = "Effect of nitric oxide - releasing compounds on phytochrome - controlled germination of Empress tree seeds",
number = "3",
volume = "26",
pages = "181",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1852"
}
Giba, Z., Grubišić, D., Todorović, S., Sajc, L., Stojaković, D.,& Konjević, R. M.. (1998). Effect of nitric oxide - releasing compounds on phytochrome - controlled germination of Empress tree seeds. in Plant Growth Regulation, 26(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1852
Giba Z, Grubišić D, Todorović S, Sajc L, Stojaković D, Konjević RM. Effect of nitric oxide - releasing compounds on phytochrome - controlled germination of Empress tree seeds. in Plant Growth Regulation. 1998;26(3):null-181.
https://hdl.handle.net/21.15107/rcub_ibiss_1852 .
Giba, Zlatko, Grubišić, Dragoljub, Todorović, S, Sajc, L, Stojaković, D, Konjević, Radomir M, "Effect of nitric oxide - releasing compounds on phytochrome - controlled germination of Empress tree seeds" in Plant Growth Regulation, 26, no. 3 (1998),
https://hdl.handle.net/21.15107/rcub_ibiss_1852 .