TY  - JOUR
AU  - Alimpić-Aradski, Ana
AU  - Olađe Pavlović, Mariana
AU  - Janošević, Dušica
AU  - Todorović, Slađana
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Pljevljakušić, Dejan
AU  - Šavikin, Katarina
AU  - Marin, Petar D.
AU  - Giweli, Abdulhmid
AU  - Duletić-Laušević, Sonja
PY  - 2023
UR  - http://www3.interscience.wiley.com/cgi-bin/jhome/5152
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6445
AB  - Introduction: The endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey-bearing plant.

Objectives: This study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro-propagated N. cyrenaica for the first time.

Materials and methods: The leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC-MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzyme-inhibiting effects were evaluated against four enzymes.

Results: The leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8-cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition.

Conclusion: This study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.
PB  - Hoboken: John Wiley and Sons
T2  - Phytochemical Analysis
T1  - Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)
IS  - 6
VL  - 34
DO  - 10.1002/pca.3257
SP  - 661
EP  - 679
ER  - 

@article{
author = "Alimpić-Aradski, Ana and Olađe Pavlović, Mariana and Janošević, Dušica and Todorović, Slađana and Gašić, Uroš and Mišić, Danijela and Pljevljakušić, Dejan and Šavikin, Katarina and Marin, Petar D. and Giweli, Abdulhmid and Duletić-Laušević, Sonja",
year = "2023",
abstract = "Introduction: The endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey-bearing plant.

Objectives: This study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro-propagated N. cyrenaica for the first time.

Materials and methods: The leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC-MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzyme-inhibiting effects were evaluated against four enzymes.

Results: The leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8-cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition.

Conclusion: This study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.",
publisher = "Hoboken: John Wiley and Sons",
journal = "Phytochemical Analysis",
title = "Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)",
number = "6",
volume = "34",
doi = "10.1002/pca.3257",
pages = "661-679"
}

Alimpić-Aradski, A., Olađe Pavlović, M., Janošević, D., Todorović, S., Gašić, U., Mišić, D., Pljevljakušić, D., Šavikin, K., Marin, P. D., Giweli, A.,& Duletić-Laušević, S.. (2023). Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae). in Phytochemical Analysis
Hoboken: John Wiley and Sons., 34(6), 661-679.
https://doi.org/10.1002/pca.3257

Alimpić-Aradski A, Olađe Pavlović M, Janošević D, Todorović S, Gašić U, Mišić D, Pljevljakušić D, Šavikin K, Marin PD, Giweli A, Duletić-Laušević S. Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae). in Phytochemical Analysis. 2023;34(6):661-679.
doi:10.1002/pca.3257 .

Alimpić-Aradski, Ana, Olađe Pavlović, Mariana, Janošević, Dušica, Todorović, Slađana, Gašić, Uroš, Mišić, Danijela, Pljevljakušić, Dejan, Šavikin, Katarina, Marin, Petar D., Giweli, Abdulhmid, Duletić-Laušević, Sonja, "Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)" in Phytochemical Analysis, 34, no. 6 (2023):661-679,
https://doi.org/10.1002/pca.3257 . .

TY  - JOUR
AU  - Mićanović, Dragica
AU  - Stojanović, Ivana D.
AU  - Koprivica, Ivan
AU  - Pejnović, Nada
AU  - Šavikin, Katarina
AU  - Ćujić-Nikolić, Nada
AU  - Saksida, Tamara
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5021
AB  - Chokeberry has exhibited cardioprotective, anti-bacterial, immunomodulating and anti-cancer properties. Chokeberry extract (CE) was tested in the model of melanoma induced by B16 cells inoculation in C57BL/6 mice.

CE treatment that began 7 days before inoculation and continued through the observation period, delayed melanoma appearance and increased infiltration of immune cells in the tumor microenvironment (TME). Levels of TNF, perforin, granzyme B and IL-1β did not differ between the CE-treated and control animals, but the TME of CE-treated mice contained more IFN-γ-producing cells and a lesser frequency of CCR5-expressing MDSC. In vitro, CE displayed no direct cytotoxicity to B16 cells. However, splenocytes isolated from CE-treated animals exerted strong cytotoxic effect on B16 cells in vitro. Neutralization of IFN-γ diminished the observed B16 death, suggesting that this effect was mediated mainly by splenocyte-derived IFN-γ.

In conclusion, pre-treatment with CE stimulated the anti-tumor immune response by enhancing IFN-γ-producing cells to act against melanoma.
PB  - Amsterdam : Elsevier
T2  - Journal of Functional Foods
T1  - Chokeberry (Aronia melanocarpa) fruit extract abrogates melanoma progression through boosting up IFN-γ-producing cells
VL  - 95
DO  - 10.1016/j.jff.2022.105185
SP  - 105185
ER  - 

@article{
author = "Mićanović, Dragica and Stojanović, Ivana D. and Koprivica, Ivan and Pejnović, Nada and Šavikin, Katarina and Ćujić-Nikolić, Nada and Saksida, Tamara",
year = "2022",
abstract = "Chokeberry has exhibited cardioprotective, anti-bacterial, immunomodulating and anti-cancer properties. Chokeberry extract (CE) was tested in the model of melanoma induced by B16 cells inoculation in C57BL/6 mice.

CE treatment that began 7 days before inoculation and continued through the observation period, delayed melanoma appearance and increased infiltration of immune cells in the tumor microenvironment (TME). Levels of TNF, perforin, granzyme B and IL-1β did not differ between the CE-treated and control animals, but the TME of CE-treated mice contained more IFN-γ-producing cells and a lesser frequency of CCR5-expressing MDSC. In vitro, CE displayed no direct cytotoxicity to B16 cells. However, splenocytes isolated from CE-treated animals exerted strong cytotoxic effect on B16 cells in vitro. Neutralization of IFN-γ diminished the observed B16 death, suggesting that this effect was mediated mainly by splenocyte-derived IFN-γ.

In conclusion, pre-treatment with CE stimulated the anti-tumor immune response by enhancing IFN-γ-producing cells to act against melanoma.",
publisher = "Amsterdam : Elsevier",
journal = "Journal of Functional Foods",
title = "Chokeberry (Aronia melanocarpa) fruit extract abrogates melanoma progression through boosting up IFN-γ-producing cells",
volume = "95",
doi = "10.1016/j.jff.2022.105185",
pages = "105185"
}

Mićanović, D., Stojanović, I. D., Koprivica, I., Pejnović, N., Šavikin, K., Ćujić-Nikolić, N.,& Saksida, T.. (2022). Chokeberry (Aronia melanocarpa) fruit extract abrogates melanoma progression through boosting up IFN-γ-producing cells. in Journal of Functional Foods
Amsterdam : Elsevier., 95, 105185.
https://doi.org/10.1016/j.jff.2022.105185

Mićanović D, Stojanović ID, Koprivica I, Pejnović N, Šavikin K, Ćujić-Nikolić N, Saksida T. Chokeberry (Aronia melanocarpa) fruit extract abrogates melanoma progression through boosting up IFN-γ-producing cells. in Journal of Functional Foods. 2022;95:105185.
doi:10.1016/j.jff.2022.105185 .

Mićanović, Dragica, Stojanović, Ivana D., Koprivica, Ivan, Pejnović, Nada, Šavikin, Katarina, Ćujić-Nikolić, Nada, Saksida, Tamara, "Chokeberry (Aronia melanocarpa) fruit extract abrogates melanoma progression through boosting up IFN-γ-producing cells" in Journal of Functional Foods, 95 (2022):105185,
https://doi.org/10.1016/j.jff.2022.105185 . .

TY  - CONF
AU  - Mićanović, Dragica
AU  - Koprivica, Ivan
AU  - Šavikin, Katarina
AU  - Šenerović, Lidija
AU  - Despotović, Sanja
AU  - Pejnović, Nada
AU  - Stojanović, Ivana D.
AU  - Saksida, Tamara
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5779
AB  - Chokeberry (Aronia melanocarpa) fruit extracts (CE) are rich in polyphenols and usually
exhibit cardioprotective, anti-viral and anti-bacterial properties1. Our aim was to
investigate the effects of CE on the immune response in vivo and in vitro, which have been
only sporadically assessed. CE, administered orally to healthy mice, exerted
immunomodulatory effects in the gut, evidenced by the altered proportion of macrophages
(Mφ), dendritic cells (DC) and T cells. CE-pretreated BALB/c mice readily eradicated
orally ingested Listeria monocytogenes due to higher proportions of Mφ and CD8 T cells
both in the gut and spleen. Additionally, phagocytosis, ROS production and the
proportions of activated Mφ and DC, as well as perforin+ cells were enhanced in CEpretreated
infected mice. Also, CE pretreatment of C57BL/6 mice inoculated with B16
cells delayed melanoma appearance and increased infiltration of immune cells in the tumor
microenvironment (TME). The TME of CE-treated mice contained more IFN-γ+ cells and
a less of tumor-promoting CCR5+ MDSC. In vitro, CE displayed no direct cytotoxicity to
B16 cells. Splenocytes isolated from CE-treated animals exerted strong cytotoxic effect on
B16 cells and this effect was diminished by neutralization of IFN-γ. In conclusion, the CE
exhibits strong immunomodulatory properties and should be consumed with care.
PB  - Belgrade: Faculty of Chemistry
C3  - Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia
T1  - Effects of chokeberry fruit water extract on immune system in mouse models of infection and melanoma
SP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5779
ER  - 

@conference{
author = "Mićanović, Dragica and Koprivica, Ivan and Šavikin, Katarina and Šenerović, Lidija and Despotović, Sanja and Pejnović, Nada and Stojanović, Ivana D. and Saksida, Tamara",
year = "2022",
abstract = "Chokeberry (Aronia melanocarpa) fruit extracts (CE) are rich in polyphenols and usually
exhibit cardioprotective, anti-viral and anti-bacterial properties1. Our aim was to
investigate the effects of CE on the immune response in vivo and in vitro, which have been
only sporadically assessed. CE, administered orally to healthy mice, exerted
immunomodulatory effects in the gut, evidenced by the altered proportion of macrophages
(Mφ), dendritic cells (DC) and T cells. CE-pretreated BALB/c mice readily eradicated
orally ingested Listeria monocytogenes due to higher proportions of Mφ and CD8 T cells
both in the gut and spleen. Additionally, phagocytosis, ROS production and the
proportions of activated Mφ and DC, as well as perforin+ cells were enhanced in CEpretreated
infected mice. Also, CE pretreatment of C57BL/6 mice inoculated with B16
cells delayed melanoma appearance and increased infiltration of immune cells in the tumor
microenvironment (TME). The TME of CE-treated mice contained more IFN-γ+ cells and
a less of tumor-promoting CCR5+ MDSC. In vitro, CE displayed no direct cytotoxicity to
B16 cells. Splenocytes isolated from CE-treated animals exerted strong cytotoxic effect on
B16 cells and this effect was diminished by neutralization of IFN-γ. In conclusion, the CE
exhibits strong immunomodulatory properties and should be consumed with care.",
publisher = "Belgrade: Faculty of Chemistry",
journal = "Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia",
title = "Effects of chokeberry fruit water extract on immune system in mouse models of infection and melanoma",
pages = "98",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5779"
}

Mićanović, D., Koprivica, I., Šavikin, K., Šenerović, L., Despotović, S., Pejnović, N., Stojanović, I. D.,& Saksida, T.. (2022). Effects of chokeberry fruit water extract on immune system in mouse models of infection and melanoma. in Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia
Belgrade: Faculty of Chemistry., 98.
https://hdl.handle.net/21.15107/rcub_ibiss_5779

Mićanović D, Koprivica I, Šavikin K, Šenerović L, Despotović S, Pejnović N, Stojanović ID, Saksida T. Effects of chokeberry fruit water extract on immune system in mouse models of infection and melanoma. in Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia. 2022;:98.
https://hdl.handle.net/21.15107/rcub_ibiss_5779 .

Mićanović, Dragica, Koprivica, Ivan, Šavikin, Katarina, Šenerović, Lidija, Despotović, Sanja, Pejnović, Nada, Stojanović, Ivana D., Saksida, Tamara, "Effects of chokeberry fruit water extract on immune system in mouse models of infection and melanoma" in Proceedings: Serbian Biochemical Society, Eleventh Conference, Scientific meeting of an international character: "Amazing Biochemistry"; 2022 Sep 22-23; Novi Sad, Serbia (2022):98,
https://hdl.handle.net/21.15107/rcub_ibiss_5779 .

TY  - CONF
AU  - Mićanović, Dragica
AU  - Saksida, Tamara
AU  - Koprivica, Ivan
AU  - Vujičić, Milica
AU  - Šavikin, Katarina
AU  - Šenerović, Lidija
AU  - Despotović, Sanja
AU  - Pejnović, Nada
AU  - Stojanović, Ivana D.
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5769
AB  - Аронија (lat. Aronia melanocarpa), воће карактеристично по тамним
бобицама опорог укуса, садржи мноштво фенолних једињења заслужних за
антиоксидативна својства ове биљне врсте. Водени екстракт плода ароније
(ВЕПА) у овом истраживању показао је проинфламацијско дејство: in vitro
је повећао фагоцитну способност макрофага и стимулисао продукцију
азот моноксида, као и диференцијацију проинфламацијских Т лимфоцита,
а in vivo након оралне примене је повећао заступљеност ефекторских Т
лимфоцита у Пејеровим плочама, као и продукцију IFN-γ на системском
нивоу. Да би се додатно испитао проинфламацијски потенцијал ВЕПА,
коришћени су експериментални мишји модели инфекције бактеријом
Listeria monocytogenes и меланома. Показано је да претретман ВЕПА не
само умањује губитак телесне масе и помаже ерадикацију инфекције, већ
и у Пејеровим плочама и слезини доводи до повећане заступљености CD8+
Т лимфоцита и CD11b+ макрофага, који се одликују већом фагоцитном
способношћу. У моделу меланома на мишевима, након седмодневног
претретмана ВЕПА индукован је меланом поткожним давањем 2.5х105 Б16
ћелија и орални третман ВЕПА је настављен до краја експеримента. Осим
што је успорио развој тумора и његову запремину, претретман је повећао
инфилтрацију имунских ћелија у тумор, као и заступљеност IFN-γ+ ћелија
унутар NK+, CD4+ и CD8+ ћелија, док је заступљеност супресорских ћелија
смањена. Ови резултати указују на потенцијал примене ароније у превенцији
стања и болести у којима је неопходна стимулација проинфламацијског
имунског одговора.
AB  - Aronija (lat. Aronia melanocarpa), voće karakteristično po tamnim bobicama oporog ukusa, sadrži mnoštvo fenolnih jedinjenja zaslužnih za antioksidativna svojstva ove biljne vrste. Vodeni ekstrakt ploda aronije (VEPA) u ovom istraživanju pokazao je proinflamacijsko dejstvo: in vitro je povećao fagocitnu sposobnost makrofaga i stimulisao produkciju azot monoksida, kao i diferencijaciju proinflamacijskih T limfocita, a in vivo nakon oralne primene je povećao zastupljenost efektorskih T limfocita u Pejerovim pločama, kao i produkciju IFN-γ na sistemskom nivou. Da bi se dodatno ispitao proinflamacijski potencijal VEPA, korišćeni su eksperimentalni mišji modeli infekcije bakterijom Listeria monocytogenes i melanoma. Pokazano je da pretretman VEPA ne samo umanjuje gubitak telesne mase i pomaže eradikaciju infekcije, već i u Pejerovim pločama i slezini dovodi do povećane zastupljenosti CD8+ T limfocita i CD11b+ makrofaga, koji se odlikuju većom fagocitnom sposobnošću. U modelu melanoma na miševima, nakon sedmodnevnog pretretmana VEPA indukovan je melanom potkožnim davanjem 2.5h105 B16 ćelija i oralni tretman VEPA je nastavljen do kraja eksperimenta. Osim što je usporio razvoj tumora i njegovu zapreminu, pretretman je povećao infiltraciju imunskih ćelija u tumor, kao i zastupljenost IFN-γ+ ćelija unutar NK+, CD4+ i CD8+ ćelija, dok je zastupljenost supresorskih ćelija smanjena. Ovi rezultati ukazuju na potencijal primene aronije u prevenciji stanja i bolesti u kojima je neophodna stimulacija proinflamacijskog imunskog odgovora.
PB  - Belgrade: Serbian Academy of Sciences and Arts
C3  - Naučni skup Svetski dan imunologije 2021; 2021 Apr 29; Belgrade, Serbia
T1  - Uticaj vodenog ekstrakta ploda aronije na imunski sistem u mišjim modelima infekcije i tumora
T1  - Утицај воденог екстракта плода ароније на имунски систем у мишјим моделима инфекције и тумора
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5769
ER  - 

@conference{
author = "Mićanović, Dragica and Saksida, Tamara and Koprivica, Ivan and Vujičić, Milica and Šavikin, Katarina and Šenerović, Lidija and Despotović, Sanja and Pejnović, Nada and Stojanović, Ivana D.",
year = "2021",
abstract = "Аронија (lat. Aronia melanocarpa), воће карактеристично по тамним
бобицама опорог укуса, садржи мноштво фенолних једињења заслужних за
антиоксидативна својства ове биљне врсте. Водени екстракт плода ароније
(ВЕПА) у овом истраживању показао је проинфламацијско дејство: in vitro
је повећао фагоцитну способност макрофага и стимулисао продукцију
азот моноксида, као и диференцијацију проинфламацијских Т лимфоцита,
а in vivo након оралне примене је повећао заступљеност ефекторских Т
лимфоцита у Пејеровим плочама, као и продукцију IFN-γ на системском
нивоу. Да би се додатно испитао проинфламацијски потенцијал ВЕПА,
коришћени су експериментални мишји модели инфекције бактеријом
Listeria monocytogenes и меланома. Показано је да претретман ВЕПА не
само умањује губитак телесне масе и помаже ерадикацију инфекције, већ
и у Пејеровим плочама и слезини доводи до повећане заступљености CD8+
Т лимфоцита и CD11b+ макрофага, који се одликују већом фагоцитном
способношћу. У моделу меланома на мишевима, након седмодневног
претретмана ВЕПА индукован је меланом поткожним давањем 2.5х105 Б16
ћелија и орални третман ВЕПА је настављен до краја експеримента. Осим
што је успорио развој тумора и његову запремину, претретман је повећао
инфилтрацију имунских ћелија у тумор, као и заступљеност IFN-γ+ ћелија
унутар NK+, CD4+ и CD8+ ћелија, док је заступљеност супресорских ћелија
смањена. Ови резултати указују на потенцијал примене ароније у превенцији
стања и болести у којима је неопходна стимулација проинфламацијског
имунског одговора., Aronija (lat. Aronia melanocarpa), voće karakteristično po tamnim bobicama oporog ukusa, sadrži mnoštvo fenolnih jedinjenja zaslužnih za antioksidativna svojstva ove biljne vrste. Vodeni ekstrakt ploda aronije (VEPA) u ovom istraživanju pokazao je proinflamacijsko dejstvo: in vitro je povećao fagocitnu sposobnost makrofaga i stimulisao produkciju azot monoksida, kao i diferencijaciju proinflamacijskih T limfocita, a in vivo nakon oralne primene je povećao zastupljenost efektorskih T limfocita u Pejerovim pločama, kao i produkciju IFN-γ na sistemskom nivou. Da bi se dodatno ispitao proinflamacijski potencijal VEPA, korišćeni su eksperimentalni mišji modeli infekcije bakterijom Listeria monocytogenes i melanoma. Pokazano je da pretretman VEPA ne samo umanjuje gubitak telesne mase i pomaže eradikaciju infekcije, već i u Pejerovim pločama i slezini dovodi do povećane zastupljenosti CD8+ T limfocita i CD11b+ makrofaga, koji se odlikuju većom fagocitnom sposobnošću. U modelu melanoma na miševima, nakon sedmodnevnog pretretmana VEPA indukovan je melanom potkožnim davanjem 2.5h105 B16 ćelija i oralni tretman VEPA je nastavljen do kraja eksperimenta. Osim što je usporio razvoj tumora i njegovu zapreminu, pretretman je povećao infiltraciju imunskih ćelija u tumor, kao i zastupljenost IFN-γ+ ćelija unutar NK+, CD4+ i CD8+ ćelija, dok je zastupljenost supresorskih ćelija smanjena. Ovi rezultati ukazuju na potencijal primene aronije u prevenciji stanja i bolesti u kojima je neophodna stimulacija proinflamacijskog imunskog odgovora.",
publisher = "Belgrade: Serbian Academy of Sciences and Arts",
journal = "Naučni skup Svetski dan imunologije 2021; 2021 Apr 29; Belgrade, Serbia",
title = "Uticaj vodenog ekstrakta ploda aronije na imunski sistem u mišjim modelima infekcije i tumora, Утицај воденог екстракта плода ароније на имунски систем у мишјим моделима инфекције и тумора",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5769"
}

Mićanović, D., Saksida, T., Koprivica, I., Vujičić, M., Šavikin, K., Šenerović, L., Despotović, S., Pejnović, N.,& Stojanović, I. D.. (2021). Uticaj vodenog ekstrakta ploda aronije na imunski sistem u mišjim modelima infekcije i tumora. in Naučni skup Svetski dan imunologije 2021; 2021 Apr 29; Belgrade, Serbia
Belgrade: Serbian Academy of Sciences and Arts..
https://hdl.handle.net/21.15107/rcub_ibiss_5769

Mićanović D, Saksida T, Koprivica I, Vujičić M, Šavikin K, Šenerović L, Despotović S, Pejnović N, Stojanović ID. Uticaj vodenog ekstrakta ploda aronije na imunski sistem u mišjim modelima infekcije i tumora. in Naučni skup Svetski dan imunologije 2021; 2021 Apr 29; Belgrade, Serbia. 2021;.
https://hdl.handle.net/21.15107/rcub_ibiss_5769 .

Mićanović, Dragica, Saksida, Tamara, Koprivica, Ivan, Vujičić, Milica, Šavikin, Katarina, Šenerović, Lidija, Despotović, Sanja, Pejnović, Nada, Stojanović, Ivana D., "Uticaj vodenog ekstrakta ploda aronije na imunski sistem u mišjim modelima infekcije i tumora" in Naučni skup Svetski dan imunologije 2021; 2021 Apr 29; Belgrade, Serbia (2021),
https://hdl.handle.net/21.15107/rcub_ibiss_5769 .

TY  - CONF
AU  - Mićanović, Dragica
AU  - Saksida, Tamara
AU  - Koprivica, Ivan
AU  - Vujičić, Milica
AU  - Despotović, Sanja
AU  - Šavikin, Katarina
AU  - Janković, Teodora
AU  - Stojanović, Ivana D.
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5783
AB  - Many plant extracts are well known for their anti-oxidant, anti-bacterial and antiinflammatory
activities including Aronia berry-derived juices and powders. In
comparison to other black berries, Aronia berries have a greater content of phenolic
constituents such as procyanidins, anthocyanins and phenolic acids with antioxidative
and anti-inflammatory properties. However, the effects of aronia berries extract on the
immune response parameters have been only sporadically assessed. When administered
orally to healthy C57BL/6 mice (50 mg/kg body weight), aronia extract exerted
immunomodulatory effects as evidenced by decreased proportion of F4/80+
macrophages, CD11c+ dendritic cells, CD4+ T helper cells, CD8+ T cytotoxic
lymphocytes and CD4+CD25- activated lymphocytes within the gut-associated
lymphoid tissue. Surprisingly, oral consumption of chokeberry extract in doses of either
200 mg/kg bw or 50 mg/kg bw in mice with multiple low dose streptozotocin-induced
type 1 diabetes resulted in the increase of blood glucose levels. Further, our study shows
that this detrimental effect on type 1 diabetes pathogenesis may be a consequence of
the pro-inflammatory nature of the extract. This is based on the evident stimulation of
macrophages and dendritic cells by the extract through up-regulation of proinflammatory
mediators such as nitric oxide, IL-12, IL-6 and TNF in vitro. Also, this
extract augmented differentiation of IFN-γ-producing T helper 1 cells in vitro.
Collectively, the obtained results imply that our particular aronia berries fruit extract
displays pro-inflammatory characteristics and that care should be taken when these
berries are to be included in the human diet.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Immunology at the Confluence of Multidisciplinary Approaches: abstract book: 2019 Dec 6-8; Belgrade, Serbia
T1  - Aronia berries fruit water extract stimulates cells of the immune system in vitro and in vivo
SP  - 130
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5783
ER  - 

@conference{
author = "Mićanović, Dragica and Saksida, Tamara and Koprivica, Ivan and Vujičić, Milica and Despotović, Sanja and Šavikin, Katarina and Janković, Teodora and Stojanović, Ivana D.",
year = "2021",
abstract = "Many plant extracts are well known for their anti-oxidant, anti-bacterial and antiinflammatory
activities including Aronia berry-derived juices and powders. In
comparison to other black berries, Aronia berries have a greater content of phenolic
constituents such as procyanidins, anthocyanins and phenolic acids with antioxidative
and anti-inflammatory properties. However, the effects of aronia berries extract on the
immune response parameters have been only sporadically assessed. When administered
orally to healthy C57BL/6 mice (50 mg/kg body weight), aronia extract exerted
immunomodulatory effects as evidenced by decreased proportion of F4/80+
macrophages, CD11c+ dendritic cells, CD4+ T helper cells, CD8+ T cytotoxic
lymphocytes and CD4+CD25- activated lymphocytes within the gut-associated
lymphoid tissue. Surprisingly, oral consumption of chokeberry extract in doses of either
200 mg/kg bw or 50 mg/kg bw in mice with multiple low dose streptozotocin-induced
type 1 diabetes resulted in the increase of blood glucose levels. Further, our study shows
that this detrimental effect on type 1 diabetes pathogenesis may be a consequence of
the pro-inflammatory nature of the extract. This is based on the evident stimulation of
macrophages and dendritic cells by the extract through up-regulation of proinflammatory
mediators such as nitric oxide, IL-12, IL-6 and TNF in vitro. Also, this
extract augmented differentiation of IFN-γ-producing T helper 1 cells in vitro.
Collectively, the obtained results imply that our particular aronia berries fruit extract
displays pro-inflammatory characteristics and that care should be taken when these
berries are to be included in the human diet.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Immunology at the Confluence of Multidisciplinary Approaches: abstract book: 2019 Dec 6-8; Belgrade, Serbia",
title = "Aronia berries fruit water extract stimulates cells of the immune system in vitro and in vivo",
pages = "130",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5783"
}

Mićanović, D., Saksida, T., Koprivica, I., Vujičić, M., Despotović, S., Šavikin, K., Janković, T.,& Stojanović, I. D.. (2021). Aronia berries fruit water extract stimulates cells of the immune system in vitro and in vivo. in Immunology at the Confluence of Multidisciplinary Approaches: abstract book: 2019 Dec 6-8; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 130.
https://hdl.handle.net/21.15107/rcub_ibiss_5783

Mićanović D, Saksida T, Koprivica I, Vujičić M, Despotović S, Šavikin K, Janković T, Stojanović ID. Aronia berries fruit water extract stimulates cells of the immune system in vitro and in vivo. in Immunology at the Confluence of Multidisciplinary Approaches: abstract book: 2019 Dec 6-8; Belgrade, Serbia. 2021;:130.
https://hdl.handle.net/21.15107/rcub_ibiss_5783 .

Mićanović, Dragica, Saksida, Tamara, Koprivica, Ivan, Vujičić, Milica, Despotović, Sanja, Šavikin, Katarina, Janković, Teodora, Stojanović, Ivana D., "Aronia berries fruit water extract stimulates cells of the immune system in vitro and in vivo" in Immunology at the Confluence of Multidisciplinary Approaches: abstract book: 2019 Dec 6-8; Belgrade, Serbia (2021):130,
https://hdl.handle.net/21.15107/rcub_ibiss_5783 .

TY  - JOUR
AU  - Oalđe, Mariana
AU  - Kolarević, Stoimir
AU  - Živković, Jelena
AU  - Alimpić Aradski, Ana
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević, Jelena
AU  - Marin, Petar D.
AU  - Šavikin, Katarina
AU  - Vuković-Gačić, Branka
AU  - Duletić-Laušević, Sonja
PY  - 2021
UR  - https://pubs.rsc.org/en/content/articlehtml/2021/fo/d1fo00447f
UR  - http://xlink.rsc.org/?DOI=D1FO00447F
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4217
AB  - This research was aimed to assess the potential of Glechoma hederacea, Hyssopus officinalis, Lavandula angustifolia, Leonurus cardiaca, Marrubium vulgare and Sideritis scardica (Lamiaceae) methanolic, ethanolic and aqueous extracts against the damaging effects of oxidative stress using different experimental models. The chemical characterization was done spectrophotometrically by quantifying total phenolics, phenolic acids, flavonoids and flavonols in the extracts, as well as by employing HPLC-DAD technique. Moreover, DPPH assay was used to assess the extracts' radical scavenging potential. Genoprotective properties of the extracts were evaluated using plasmid pUC19 Escherichia coli XL1-Blue, whereas their antigenotoxic potential was determined using Salmonella typhimurium TA1535/pSK1002 and normal human lung fibroblasts. All of the extracts showed antioxidant activity in DPPH assay. Furthermore, the results have shown that aqueous extracts provided the best protection for plasmid DNA, while alcoholic extracts most effectively contributed to the preservation of prokaryotic DNA. Additionally, each of the tested samples significantly protected the eukaryotic cells against genomic damages. Finally, despite not showing exceptional results in DPPH assay, S. scardica extracts are regarded as the most favorable in maintaining the integrity of DNA, which might be due to high quantities of phenolics such as quercetin (up to 17.95 mg g-1), naringin (up to 5.07 mg g-1) and luteolin-7-O-glucoside (up to 3.54 mg g-1). Overall, this comprehensive concept highlights the ability of these Lamiaceae species to safeguard the DNA from reactive oxygen species, to curtail the inflicted damage and also improve the efficiency of the DNA repair mechanisms, while emphasizing the importance of polyphenols as their active principles.
PB  - Royal Society of Chemistry (RSC)
T2  - Food & Function
T1  - A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro
IS  - 7
VL  - 12
DO  - 10.1039/d1fo00447f
SP  - 3233
EP  - 3245
ER  - 

@article{
author = "Oalđe, Mariana and Kolarević, Stoimir and Živković, Jelena and Alimpić Aradski, Ana and Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Đorđević, Jelena and Marin, Petar D. and Šavikin, Katarina and Vuković-Gačić, Branka and Duletić-Laušević, Sonja",
year = "2021",
abstract = "This research was aimed to assess the potential of Glechoma hederacea, Hyssopus officinalis, Lavandula angustifolia, Leonurus cardiaca, Marrubium vulgare and Sideritis scardica (Lamiaceae) methanolic, ethanolic and aqueous extracts against the damaging effects of oxidative stress using different experimental models. The chemical characterization was done spectrophotometrically by quantifying total phenolics, phenolic acids, flavonoids and flavonols in the extracts, as well as by employing HPLC-DAD technique. Moreover, DPPH assay was used to assess the extracts' radical scavenging potential. Genoprotective properties of the extracts were evaluated using plasmid pUC19 Escherichia coli XL1-Blue, whereas their antigenotoxic potential was determined using Salmonella typhimurium TA1535/pSK1002 and normal human lung fibroblasts. All of the extracts showed antioxidant activity in DPPH assay. Furthermore, the results have shown that aqueous extracts provided the best protection for plasmid DNA, while alcoholic extracts most effectively contributed to the preservation of prokaryotic DNA. Additionally, each of the tested samples significantly protected the eukaryotic cells against genomic damages. Finally, despite not showing exceptional results in DPPH assay, S. scardica extracts are regarded as the most favorable in maintaining the integrity of DNA, which might be due to high quantities of phenolics such as quercetin (up to 17.95 mg g-1), naringin (up to 5.07 mg g-1) and luteolin-7-O-glucoside (up to 3.54 mg g-1). Overall, this comprehensive concept highlights the ability of these Lamiaceae species to safeguard the DNA from reactive oxygen species, to curtail the inflicted damage and also improve the efficiency of the DNA repair mechanisms, while emphasizing the importance of polyphenols as their active principles.",
publisher = "Royal Society of Chemistry (RSC)",
journal = "Food & Function",
title = "A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro",
number = "7",
volume = "12",
doi = "10.1039/d1fo00447f",
pages = "3233-3245"
}

Oalđe, M., Kolarević, S., Živković, J., Alimpić Aradski, A., Jovanović Marić, J., Kračun-Kolarević, M., Đorđević, J., Marin, P. D., Šavikin, K., Vuković-Gačić, B.,& Duletić-Laušević, S.. (2021). A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro. in Food & Function
Royal Society of Chemistry (RSC)., 12(7), 3233-3245.
https://doi.org/10.1039/d1fo00447f

Oalđe M, Kolarević S, Živković J, Alimpić Aradski A, Jovanović Marić J, Kračun-Kolarević M, Đorđević J, Marin PD, Šavikin K, Vuković-Gačić B, Duletić-Laušević S. A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro. in Food & Function. 2021;12(7):3233-3245.
doi:10.1039/d1fo00447f .

Oalđe, Mariana, Kolarević, Stoimir, Živković, Jelena, Alimpić Aradski, Ana, Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Đorđević, Jelena, Marin, Petar D., Šavikin, Katarina, Vuković-Gačić, Branka, Duletić-Laušević, Sonja, "A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro" in Food & Function, 12, no. 7 (2021):3233-3245,
https://doi.org/10.1039/d1fo00447f . .

TY  - JOUR
AU  - Savić, Aleksandra
AU  - Alimpić Aradski, Ana
AU  - Živković, Jelena
AU  - Šavikin, Katarina
AU  - Jarić, Snežana
AU  - Marin, Petar
AU  - Duletić-Laušević, Sonja
PY  - 2021
UR  - http://journal.pan.olsztyn.pl/Phenolic-Composition-and-Antioxidant-and-Antineurodegenerative-Potential-of-Methanolic,138320,0,2.html
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4416
AB  - Pear (Pyrus communis L.) is consumed as fresh fruit, in numerous food products, and also used as a traditional remedy in various countries, including Serbia. In search of bioactive compounds, six traditional pear varieties (‘Vidovača’, ‘Lubeničarka’, ‘Karamanka’, ‘Jeribasma’, ‘Lončara’, ‘Takiša’) and wild pear from Serbia were investigated and compared with a commercial variety (‘Williams Bartlett’). The aim of this study was to determine the total phenolic and flavonoid contents, phenolic composition, antioxidant capacity, and antineurodegenerative activities of methanolic extracts of peel, flesh, and mixed peel and flesh of pear fruits. Phenolic composition of extracts was determined with HPLC-DAD, while the antioxidant activity of extracts was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and ferric-reducing antioxidant power (FRAP) assays. Bearing in mind that oxidative stress is closely linked to neurodegeneration, the antineurodegenerative potential of the extracts was assessed by the inhibition of acetycholineserase (AChE) and tyrosinase (TYR) activities. The extracts of traditional varieties, particularly peel extracts, had a high content of phenolics, as well as significant antioxidant and moderate antineurodegenerative potential, compared to the commercial variety. The highest contents of total flavonoids and individual compounds, such as arbutin and chlorogenic acid, as well as the strongest antioxidant and TYR inhibitory activities were reported for the ‘Takiša’ peel extract. The performed analyses have revealed that fruits of traditional Serbian pear varieties are rich in bioactive components and could be used as functional food and for possible nutraceutical applications, to prevent diseases induced by oxidative stress.
T2  - Polish Journal of Food and Nutrition Sciences
T1  - Phenolic Composition, and Antioxidant and Antineurodegenerative Potential of Methanolic Extracts of Fruit Peel and Flesh of Pear Varieties from Serbia
IS  - 2
VL  - 71
DO  - 10.31883/pjfns/138320
SP  - 225
EP  - 236
ER  - 

@article{
author = "Savić, Aleksandra and Alimpić Aradski, Ana and Živković, Jelena and Šavikin, Katarina and Jarić, Snežana and Marin, Petar and Duletić-Laušević, Sonja",
year = "2021",
abstract = "Pear (Pyrus communis L.) is consumed as fresh fruit, in numerous food products, and also used as a traditional remedy in various countries, including Serbia. In search of bioactive compounds, six traditional pear varieties (‘Vidovača’, ‘Lubeničarka’, ‘Karamanka’, ‘Jeribasma’, ‘Lončara’, ‘Takiša’) and wild pear from Serbia were investigated and compared with a commercial variety (‘Williams Bartlett’). The aim of this study was to determine the total phenolic and flavonoid contents, phenolic composition, antioxidant capacity, and antineurodegenerative activities of methanolic extracts of peel, flesh, and mixed peel and flesh of pear fruits. Phenolic composition of extracts was determined with HPLC-DAD, while the antioxidant activity of extracts was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and ferric-reducing antioxidant power (FRAP) assays. Bearing in mind that oxidative stress is closely linked to neurodegeneration, the antineurodegenerative potential of the extracts was assessed by the inhibition of acetycholineserase (AChE) and tyrosinase (TYR) activities. The extracts of traditional varieties, particularly peel extracts, had a high content of phenolics, as well as significant antioxidant and moderate antineurodegenerative potential, compared to the commercial variety. The highest contents of total flavonoids and individual compounds, such as arbutin and chlorogenic acid, as well as the strongest antioxidant and TYR inhibitory activities were reported for the ‘Takiša’ peel extract. The performed analyses have revealed that fruits of traditional Serbian pear varieties are rich in bioactive components and could be used as functional food and for possible nutraceutical applications, to prevent diseases induced by oxidative stress.",
journal = "Polish Journal of Food and Nutrition Sciences",
title = "Phenolic Composition, and Antioxidant and Antineurodegenerative Potential of Methanolic Extracts of Fruit Peel and Flesh of Pear Varieties from Serbia",
number = "2",
volume = "71",
doi = "10.31883/pjfns/138320",
pages = "225-236"
}

Savić, A., Alimpić Aradski, A., Živković, J., Šavikin, K., Jarić, S., Marin, P.,& Duletić-Laušević, S.. (2021). Phenolic Composition, and Antioxidant and Antineurodegenerative Potential of Methanolic Extracts of Fruit Peel and Flesh of Pear Varieties from Serbia. in Polish Journal of Food and Nutrition Sciences, 71(2), 225-236.
https://doi.org/10.31883/pjfns/138320

Savić A, Alimpić Aradski A, Živković J, Šavikin K, Jarić S, Marin P, Duletić-Laušević S. Phenolic Composition, and Antioxidant and Antineurodegenerative Potential of Methanolic Extracts of Fruit Peel and Flesh of Pear Varieties from Serbia. in Polish Journal of Food and Nutrition Sciences. 2021;71(2):225-236.
doi:10.31883/pjfns/138320 .

Savić, Aleksandra, Alimpić Aradski, Ana, Živković, Jelena, Šavikin, Katarina, Jarić, Snežana, Marin, Petar, Duletić-Laušević, Sonja, "Phenolic Composition, and Antioxidant and Antineurodegenerative Potential of Methanolic Extracts of Fruit Peel and Flesh of Pear Varieties from Serbia" in Polish Journal of Food and Nutrition Sciences, 71, no. 2 (2021):225-236,
https://doi.org/10.31883/pjfns/138320 . .

TY  - JOUR
AU  - Oalđe Pavlović, Mariana
AU  - Kolarević, Stoimir
AU  - Đorđević, Jelena
AU  - Jovanović Marić, Jovana
AU  - Lunić, Tanja
AU  - Mandić, Marija
AU  - Kračun-Kolarević, Margareta
AU  - Živković, Jelena
AU  - Alimpić Aradski, Ana
AU  - Marin, Petar D.
AU  - Šavikin, Katarina
AU  - Vuković-Gačić, Branka
AU  - Božić Nedeljković, Biljana
AU  - Duletić-Laušević, Sonja
PY  - 2021
UR  - https://www.mdpi.com/2223-7747/10/11/2306
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4654
AB  - This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha × piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.
PB  - Basel: MDPI
T2  - Plants
T1  - A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species
IS  - 11
VL  - 10
DO  - 10.3390/plants10112306
SP  - 2306
ER  - 

@article{
author = "Oalđe Pavlović, Mariana and Kolarević, Stoimir and Đorđević, Jelena and Jovanović Marić, Jovana and Lunić, Tanja and Mandić, Marija and Kračun-Kolarević, Margareta and Živković, Jelena and Alimpić Aradski, Ana and Marin, Petar D. and Šavikin, Katarina and Vuković-Gačić, Branka and Božić Nedeljković, Biljana and Duletić-Laušević, Sonja",
year = "2021",
abstract = "This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha × piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species",
number = "11",
volume = "10",
doi = "10.3390/plants10112306",
pages = "2306"
}

Oalđe Pavlović, M., Kolarević, S., Đorđević, J., Jovanović Marić, J., Lunić, T., Mandić, M., Kračun-Kolarević, M., Živković, J., Alimpić Aradski, A., Marin, P. D., Šavikin, K., Vuković-Gačić, B., Božić Nedeljković, B.,& Duletić-Laušević, S.. (2021). A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants
Basel: MDPI., 10(11), 2306.
https://doi.org/10.3390/plants10112306

Oalđe Pavlović M, Kolarević S, Đorđević J, Jovanović Marić J, Lunić T, Mandić M, Kračun-Kolarević M, Živković J, Alimpić Aradski A, Marin PD, Šavikin K, Vuković-Gačić B, Božić Nedeljković B, Duletić-Laušević S. A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants. 2021;10(11):2306.
doi:10.3390/plants10112306 .

Oalđe Pavlović, Mariana, Kolarević, Stoimir, Đorđević, Jelena, Jovanović Marić, Jovana, Lunić, Tanja, Mandić, Marija, Kračun-Kolarević, Margareta, Živković, Jelena, Alimpić Aradski, Ana, Marin, Petar D., Šavikin, Katarina, Vuković-Gačić, Branka, Božić Nedeljković, Biljana, Duletić-Laušević, Sonja, "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species" in Plants, 10, no. 11 (2021):2306,
https://doi.org/10.3390/plants10112306 . .

TY  - JOUR
AU  - Mićanović, Dragica
AU  - Saksida, Tamara
AU  - Koprivica, Ivan
AU  - Vujičić, Milica
AU  - Despotović, Sanja
AU  - Šavikin, Katarina
AU  - Janković, Teodora
AU  - Stojanović, Ivana D.
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3591
AB  - Chokeberry (Aronia melanocarpa) is known for its anti-oxidant, anti-inflammatory and anti-diabetic properties. Since the effects of chokeberry extract on the immune response have been only sporadically assessed, our aim was to investigate chokeberry fruit water extract on the immune response in vivo and in vitro. When administered orally to healthy mice, the extract exerted immunomodulatory effects in the gut evidenced by the altered proportion of macrophages, dendritic cells and T cells. Importantly, oral consumption of the chokeberry extract resulted in blood glucose level increase in C57BL/6 mice with chemically-induced diabetes. These in vivo results were corroborated by observed up-regulation of nitric oxide and interelukin-1β production in macrophages and dendritic cells, up-regulated phagocytic activity of macrophages, increased T and B lymphocytes proportions and differentiation of interferon-γ-producing T cells in vitro. The obtained results imply that our chokeberry extract stimulates pro-inflammatory properties in immune cells of innate and adaptive immunity.
PB  - Elsevier Ltd.
T2  - Journal of Functional Foods
T1  - Chokeberry (Aronia melanocarpa) fruit extract modulates immune response in vivo and in vitro
VL  - 66
DO  - 10.1016/j.jff.2020.103836
SP  - 103836
ER  - 

@article{
author = "Mićanović, Dragica and Saksida, Tamara and Koprivica, Ivan and Vujičić, Milica and Despotović, Sanja and Šavikin, Katarina and Janković, Teodora and Stojanović, Ivana D.",
year = "2020",
abstract = "Chokeberry (Aronia melanocarpa) is known for its anti-oxidant, anti-inflammatory and anti-diabetic properties. Since the effects of chokeberry extract on the immune response have been only sporadically assessed, our aim was to investigate chokeberry fruit water extract on the immune response in vivo and in vitro. When administered orally to healthy mice, the extract exerted immunomodulatory effects in the gut evidenced by the altered proportion of macrophages, dendritic cells and T cells. Importantly, oral consumption of the chokeberry extract resulted in blood glucose level increase in C57BL/6 mice with chemically-induced diabetes. These in vivo results were corroborated by observed up-regulation of nitric oxide and interelukin-1β production in macrophages and dendritic cells, up-regulated phagocytic activity of macrophages, increased T and B lymphocytes proportions and differentiation of interferon-γ-producing T cells in vitro. The obtained results imply that our chokeberry extract stimulates pro-inflammatory properties in immune cells of innate and adaptive immunity.",
publisher = "Elsevier Ltd.",
journal = "Journal of Functional Foods",
title = "Chokeberry (Aronia melanocarpa) fruit extract modulates immune response in vivo and in vitro",
volume = "66",
doi = "10.1016/j.jff.2020.103836",
pages = "103836"
}

Mićanović, D., Saksida, T., Koprivica, I., Vujičić, M., Despotović, S., Šavikin, K., Janković, T.,& Stojanović, I. D.. (2020). Chokeberry (Aronia melanocarpa) fruit extract modulates immune response in vivo and in vitro. in Journal of Functional Foods
Elsevier Ltd.., 66, 103836.
https://doi.org/10.1016/j.jff.2020.103836

Mićanović D, Saksida T, Koprivica I, Vujičić M, Despotović S, Šavikin K, Janković T, Stojanović ID. Chokeberry (Aronia melanocarpa) fruit extract modulates immune response in vivo and in vitro. in Journal of Functional Foods. 2020;66:103836.
doi:10.1016/j.jff.2020.103836 .

Mićanović, Dragica, Saksida, Tamara, Koprivica, Ivan, Vujičić, Milica, Despotović, Sanja, Šavikin, Katarina, Janković, Teodora, Stojanović, Ivana D., "Chokeberry (Aronia melanocarpa) fruit extract modulates immune response in vivo and in vitro" in Journal of Functional Foods, 66 (2020):103836,
https://doi.org/10.1016/j.jff.2020.103836 . .

TY  - JOUR
AU  - Mićanović, Dragica
AU  - Saksida, Tamara
AU  - Koprivica, Ivan
AU  - Šenerović, Lidija
AU  - Morić, Ivana
AU  - Šavikin, Katarina
AU  - Menković, Nebojša
AU  - Pejnović, Nada
AU  - Stojanović, Ivana D.
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3834
AB  - Chokeberry (Aronia melanocarpa) fruit extracts (CE) are rich in polyphenols and usually exhibit immunomodulatory, anti-viral and anti-bacterial effects. We have previously shown that the CE used in this study activated macrophages and stimulated effector T cell differentiation in vitro. When applied orally to healthy mice, CE increased the proportion of CD11c+ dendritic cells in the gut-associated lymphoid tissue. CE-pretreated BALB/c mice readily eradicated orally ingested Listeria monocytogenes as evidenced by a slighter decrease in body weight and number of bacteria recovered from the spleen and reduced spleen size compared to the control infected mice. CE pretreatment in infected mice resulted in higher proportions of CD11b+ macrophages and CD8+ cytotoxic T cells both in the gut and the spleen. Phagocytosis, reactive oxygen species production and the proportions of activated CD86+ macrophages (CD11b+) and dendritic cells (CD11c+) was also enhanced in CE-pretreated infected mice. Further, the expression of inducible nitric oxide synthase and IL-6 was increased in CE-pretreated infected mice and the similar results were obtained in peritoneal macrophages in vitro. This effect of CE was associated with increased phosphorylation of IκB and Notch1 production. Finally, CE pretreatment elevated the proportion of perforin-producing cells in the spleen compared to control infected mice. This study demonstrates that prophylactic treatment with CE leads to more rapid eradication of bacterial infection with Listeria monocytogenes predominantly through increased activity of myeloid cells in the gut and in the spleen.
PB  - United Kingdom : Royal Society of Chemistry
T2  - Food and Function
T1  - Immunomodulatory activity and protective effects of chokeberry fruit extract on Listeria monocytogenes infection in mice
IS  - 9
VL  - 11
DO  - 10.1039/D0FO00946F
SP  - 7793
EP  - 7803
ER  - 

@article{
author = "Mićanović, Dragica and Saksida, Tamara and Koprivica, Ivan and Šenerović, Lidija and Morić, Ivana and Šavikin, Katarina and Menković, Nebojša and Pejnović, Nada and Stojanović, Ivana D.",
year = "2020",
abstract = "Chokeberry (Aronia melanocarpa) fruit extracts (CE) are rich in polyphenols and usually exhibit immunomodulatory, anti-viral and anti-bacterial effects. We have previously shown that the CE used in this study activated macrophages and stimulated effector T cell differentiation in vitro. When applied orally to healthy mice, CE increased the proportion of CD11c+ dendritic cells in the gut-associated lymphoid tissue. CE-pretreated BALB/c mice readily eradicated orally ingested Listeria monocytogenes as evidenced by a slighter decrease in body weight and number of bacteria recovered from the spleen and reduced spleen size compared to the control infected mice. CE pretreatment in infected mice resulted in higher proportions of CD11b+ macrophages and CD8+ cytotoxic T cells both in the gut and the spleen. Phagocytosis, reactive oxygen species production and the proportions of activated CD86+ macrophages (CD11b+) and dendritic cells (CD11c+) was also enhanced in CE-pretreated infected mice. Further, the expression of inducible nitric oxide synthase and IL-6 was increased in CE-pretreated infected mice and the similar results were obtained in peritoneal macrophages in vitro. This effect of CE was associated with increased phosphorylation of IκB and Notch1 production. Finally, CE pretreatment elevated the proportion of perforin-producing cells in the spleen compared to control infected mice. This study demonstrates that prophylactic treatment with CE leads to more rapid eradication of bacterial infection with Listeria monocytogenes predominantly through increased activity of myeloid cells in the gut and in the spleen.",
publisher = "United Kingdom : Royal Society of Chemistry",
journal = "Food and Function",
title = "Immunomodulatory activity and protective effects of chokeberry fruit extract on Listeria monocytogenes infection in mice",
number = "9",
volume = "11",
doi = "10.1039/D0FO00946F",
pages = "7793-7803"
}

Mićanović, D., Saksida, T., Koprivica, I., Šenerović, L., Morić, I., Šavikin, K., Menković, N., Pejnović, N.,& Stojanović, I. D.. (2020). Immunomodulatory activity and protective effects of chokeberry fruit extract on Listeria monocytogenes infection in mice. in Food and Function
United Kingdom : Royal Society of Chemistry., 11(9), 7793-7803.
https://doi.org/10.1039/D0FO00946F

Mićanović D, Saksida T, Koprivica I, Šenerović L, Morić I, Šavikin K, Menković N, Pejnović N, Stojanović ID. Immunomodulatory activity and protective effects of chokeberry fruit extract on Listeria monocytogenes infection in mice. in Food and Function. 2020;11(9):7793-7803.
doi:10.1039/D0FO00946F .

Mićanović, Dragica, Saksida, Tamara, Koprivica, Ivan, Šenerović, Lidija, Morić, Ivana, Šavikin, Katarina, Menković, Nebojša, Pejnović, Nada, Stojanović, Ivana D., "Immunomodulatory activity and protective effects of chokeberry fruit extract on Listeria monocytogenes infection in mice" in Food and Function, 11, no. 9 (2020):7793-7803,
https://doi.org/10.1039/D0FO00946F . .

TY  - JOUR
AU  - Živković, Jelena
AU  - Ilić, Milan
AU  - Šavikin, Katarina
AU  - Zdunić, Gordana
AU  - Ilić, Aleksandra
AU  - Stojković, Dejan
PY  - 2020
UR  - https://www.frontiersin.org/article/10.3389/fphar.2020.01020/full
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32733251
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7360817
UR  - https://radar.ibiss.bg.ac.rs/123456789/3849
AB  - Balkan Peninsula is one of the most important biodiversity centers in Europe. Despite that, the usage of plant species in the traditional medicine of some Balkan regions remained largely unexplored in the past. This study aimed to collect and document data on the traditional use of medicinal plants in Pčinja district in South-Eastern Serbia, which is among the least developed regions in Serbia. Also, comparison with data collected by Dr. Jovan Tucakov, in a book called Herbal therapy was conducted. The survey was carried out using semi-structured interviews and 113 informants were interviewed. Quantitative ethnobotany factors were calculated, allowing us discussing the results. The informants reported data on 86 medicinal plants belonging to 43 families in Pčinja district. Lamiaceae, Asteraceae, and Rosaceae were the dominant locally used families. The species with the highest number of use reports were Mentha piperita, Matricaria chamomilla, and Hypericum perforatum. Gastrointestinal ailments, respiratory problems and skin diseases were the most frequently reported indications. Usually, the administration was primarily oral followed by topical applications. Leaves were dominantly exploited plant parts and the most frequent preparation form was infusion. Medicinal plants in Pčinja district are mainly used as a mode of primary health care for treating minor health issues. After comparing our results with the ones collected half a century ago by Dr. Jovan Tucakov we can conclude that plant species mentioned in our investigation previously had a much wider spectrum of application.
PB  - Frontiers Media S.A.
T2  - Frontiers in Pharmacology
T1  - Traditional Use of Medicinal Plants in South-Eastern Serbia (Pčinja District): Ethnopharmacological Investigation on the Current Status and Comparison With Half a Century Old Data.
VL  - 11
DO  - 10.3389/fphar.2020.01020
SP  - 1020
ER  - 

@article{
author = "Živković, Jelena and Ilić, Milan and Šavikin, Katarina and Zdunić, Gordana and Ilić, Aleksandra and Stojković, Dejan",
year = "2020",
abstract = "Balkan Peninsula is one of the most important biodiversity centers in Europe. Despite that, the usage of plant species in the traditional medicine of some Balkan regions remained largely unexplored in the past. This study aimed to collect and document data on the traditional use of medicinal plants in Pčinja district in South-Eastern Serbia, which is among the least developed regions in Serbia. Also, comparison with data collected by Dr. Jovan Tucakov, in a book called Herbal therapy was conducted. The survey was carried out using semi-structured interviews and 113 informants were interviewed. Quantitative ethnobotany factors were calculated, allowing us discussing the results. The informants reported data on 86 medicinal plants belonging to 43 families in Pčinja district. Lamiaceae, Asteraceae, and Rosaceae were the dominant locally used families. The species with the highest number of use reports were Mentha piperita, Matricaria chamomilla, and Hypericum perforatum. Gastrointestinal ailments, respiratory problems and skin diseases were the most frequently reported indications. Usually, the administration was primarily oral followed by topical applications. Leaves were dominantly exploited plant parts and the most frequent preparation form was infusion. Medicinal plants in Pčinja district are mainly used as a mode of primary health care for treating minor health issues. After comparing our results with the ones collected half a century ago by Dr. Jovan Tucakov we can conclude that plant species mentioned in our investigation previously had a much wider spectrum of application.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Pharmacology",
title = "Traditional Use of Medicinal Plants in South-Eastern Serbia (Pčinja District): Ethnopharmacological Investigation on the Current Status and Comparison With Half a Century Old Data.",
volume = "11",
doi = "10.3389/fphar.2020.01020",
pages = "1020"
}

Živković, J., Ilić, M., Šavikin, K., Zdunić, G., Ilić, A.,& Stojković, D.. (2020). Traditional Use of Medicinal Plants in South-Eastern Serbia (Pčinja District): Ethnopharmacological Investigation on the Current Status and Comparison With Half a Century Old Data.. in Frontiers in Pharmacology
Frontiers Media S.A.., 11, 1020.
https://doi.org/10.3389/fphar.2020.01020

Živković J, Ilić M, Šavikin K, Zdunić G, Ilić A, Stojković D. Traditional Use of Medicinal Plants in South-Eastern Serbia (Pčinja District): Ethnopharmacological Investigation on the Current Status and Comparison With Half a Century Old Data.. in Frontiers in Pharmacology. 2020;11:1020.
doi:10.3389/fphar.2020.01020 .

Živković, Jelena, Ilić, Milan, Šavikin, Katarina, Zdunić, Gordana, Ilić, Aleksandra, Stojković, Dejan, "Traditional Use of Medicinal Plants in South-Eastern Serbia (Pčinja District): Ethnopharmacological Investigation on the Current Status and Comparison With Half a Century Old Data." in Frontiers in Pharmacology, 11 (2020):1020,
https://doi.org/10.3389/fphar.2020.01020 . .

TY  - JOUR
AU  - Zdunić, Gordana
AU  - Alimpić Aradski, Ana
AU  - Gođevac, Dejan
AU  - Živković, Jelena
AU  - Duletić Laušević, Sonja
AU  - Krstić Milošević, Dijana
AU  - Šavikin, Katarina
PY  - 2020
UR  - internal-pdf://Aronija 2020.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3619
AB  - Chokeberries (Aronia melanocarpa (Michx.) Elliot have recently become one of the most popular and widely used berry fruits, and there are numerous studies dealing with the investigation of their chemical composition and biological activities. Unlike berries, chokeberry leaves were far less investigated. Therefore, the aim of this study was to conduct a bioactivity-based analysis of chokeberry leaves collected after berries were harvested. Chemical analysis of hydroethanolic chokeberry extract and its ethyl acetate, n-butanol, and water fractions was performed. Antioxidant, hypoglycemic, and antineurodegenerative activities of the obtained samples were assessed by in vitro tests. The highest total phenolics content was found in n-butanol fraction (221.5 mg gallic acid equivalents/g dw). Fourteen compounds (six phenolic acids and eight flavonoids) were identified in the samples. Caffeoylquinic acid derivatives and quercetin glycosides were dominant phenolic compounds. The highest content of phenolic acids, as well as flavonoids, was recorded in n-butanol fraction (80.75 mg/g dw and 47.79 mg/g dw, respectively). This fraction was the most active in 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assays. Also, the n-butanol fraction was the most effective inhibitor of acetylcholinesterase (AChE) and tyrosinase (TYR) enzymes. All samples showed a better inhibitory effect on α-glucosidase enzyme with inhibition concentrations IC50 = 4.21 - 9.22 μg/mL, than α-amylase enzymes (IC50 = 14.36 - 88.61 μg/mL). Pearson’s correlation between the content of individual phenolics and the results of biological tests was performed. Thus, hyperoside and isoquercitrin showed a significant correlation with DPPH and ABTS tests, caffeoylquinic acids with FRAP test, quercetin 3-O-vicianoside with β-carotene assay, hyperoside and isoquercitrin with α-amylase inhibition activity, and isorhamnetin 3-O-rutinoside with α-glucosidase inhibition assay. Considering the obtained results, chokeberry leaves represent a valuable natural source of phenolic compounds with promising potential for the development of new products with beneficial effects on human health.
T2  - Industrial Crops and Products
T1  - In vitro hypoglycemic, antioxidant and antineurodegenerative activity of chokeberry (Aronia melanocarpa) leaves
VL  - 148
DO  - 10.1016/j.indcrop.2020.112328
SP  - 112328
ER  - 

@article{
author = "Zdunić, Gordana and Alimpić Aradski, Ana and Gođevac, Dejan and Živković, Jelena and Duletić Laušević, Sonja and Krstić Milošević, Dijana and Šavikin, Katarina",
year = "2020",
abstract = "Chokeberries (Aronia melanocarpa (Michx.) Elliot have recently become one of the most popular and widely used berry fruits, and there are numerous studies dealing with the investigation of their chemical composition and biological activities. Unlike berries, chokeberry leaves were far less investigated. Therefore, the aim of this study was to conduct a bioactivity-based analysis of chokeberry leaves collected after berries were harvested. Chemical analysis of hydroethanolic chokeberry extract and its ethyl acetate, n-butanol, and water fractions was performed. Antioxidant, hypoglycemic, and antineurodegenerative activities of the obtained samples were assessed by in vitro tests. The highest total phenolics content was found in n-butanol fraction (221.5 mg gallic acid equivalents/g dw). Fourteen compounds (six phenolic acids and eight flavonoids) were identified in the samples. Caffeoylquinic acid derivatives and quercetin glycosides were dominant phenolic compounds. The highest content of phenolic acids, as well as flavonoids, was recorded in n-butanol fraction (80.75 mg/g dw and 47.79 mg/g dw, respectively). This fraction was the most active in 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assays. Also, the n-butanol fraction was the most effective inhibitor of acetylcholinesterase (AChE) and tyrosinase (TYR) enzymes. All samples showed a better inhibitory effect on α-glucosidase enzyme with inhibition concentrations IC50 = 4.21 - 9.22 μg/mL, than α-amylase enzymes (IC50 = 14.36 - 88.61 μg/mL). Pearson’s correlation between the content of individual phenolics and the results of biological tests was performed. Thus, hyperoside and isoquercitrin showed a significant correlation with DPPH and ABTS tests, caffeoylquinic acids with FRAP test, quercetin 3-O-vicianoside with β-carotene assay, hyperoside and isoquercitrin with α-amylase inhibition activity, and isorhamnetin 3-O-rutinoside with α-glucosidase inhibition assay. Considering the obtained results, chokeberry leaves represent a valuable natural source of phenolic compounds with promising potential for the development of new products with beneficial effects on human health.",
journal = "Industrial Crops and Products",
title = "In vitro hypoglycemic, antioxidant and antineurodegenerative activity of chokeberry (Aronia melanocarpa) leaves",
volume = "148",
doi = "10.1016/j.indcrop.2020.112328",
pages = "112328"
}

Zdunić, G., Alimpić Aradski, A., Gođevac, D., Živković, J., Duletić Laušević, S., Krstić Milošević, D.,& Šavikin, K.. (2020). In vitro hypoglycemic, antioxidant and antineurodegenerative activity of chokeberry (Aronia melanocarpa) leaves. in Industrial Crops and Products, 148, 112328.
https://doi.org/10.1016/j.indcrop.2020.112328

Zdunić G, Alimpić Aradski A, Gođevac D, Živković J, Duletić Laušević S, Krstić Milošević D, Šavikin K. In vitro hypoglycemic, antioxidant and antineurodegenerative activity of chokeberry (Aronia melanocarpa) leaves. in Industrial Crops and Products. 2020;148:112328.
doi:10.1016/j.indcrop.2020.112328 .

Zdunić, Gordana, Alimpić Aradski, Ana, Gođevac, Dejan, Živković, Jelena, Duletić Laušević, Sonja, Krstić Milošević, Dijana, Šavikin, Katarina, "In vitro hypoglycemic, antioxidant and antineurodegenerative activity of chokeberry (Aronia melanocarpa) leaves" in Industrial Crops and Products, 148 (2020):112328,
https://doi.org/10.1016/j.indcrop.2020.112328 . .

TY  - CONF
AU  - Živković, Jelena
AU  - Ilić, Milan
AU  - Stojković, Dejan
AU  - Šavikin, Katarina
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4371
AB  - Balkan Peninsula is one of the most important biodiversity centers in Europe. Despite 
that, the usage of plant species in traditional medicine of some Balkan regions remained 
largely unexplored in the past. This study aimed to collect and document data on the 
traditional use of medicinal plants in Pčinja district in South-Eastern Serbia, which is 
among the least developed regions in Serbia. The survey was carried out using semi structured interviews and 113 informants were interviewed. Quantitative ethnobotany 
factors were calculated, allowing us discussing the results.
The informants reported data on 86 medicinal plants belonging to 43 families. 
The most dominant botanical families were Lamiaceae (30% of species), Asteraceae 
(26% of species) and Rosaceae (19% of species). Species with the highest number of 
use reports were Mentha piperita, Hypericum perforatum, Matricaria chamomilla, Salvia
officinalis, Urtica dioica, Thymus serpyllum and Tilia cordata. The majority of the plants 
were reported to have 1-3 different usages and the species with most diverse uses were 
Hypericum perforatum and Urtica dioica. In Pčinja district folk medicine is primarily used 
for healing minor diseases with some exceptions. The most frequent medicinal uses 
were for treating diseases of the digestive system, respiratory system and diseases of 
the skin and subcutaneous tissue, followed by general and unspecified diseases (such 
as pain, fever and health prevention). Usually, the administration was primarily oral 
followed by topical applications. Leaves were dominantly exploited plant parts and 
the most frequent preparation form was infusion. The current study represents a useful 
documentation which can contribute to preserving ethnobotanical knowledge in 
South-Eastern Serbia. For some well-known plant species new applications have been 
recorded. This can present a good starting point for new investigations
PB  - Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade
C3  - The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia
T1  - Ethnobotanical study of medicinal plants traditionally used in South-East Serbia (Pčinja district)
SP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4371
ER  - 

@conference{
author = "Živković, Jelena and Ilić, Milan and Stojković, Dejan and Šavikin, Katarina",
year = "2019",
abstract = "Balkan Peninsula is one of the most important biodiversity centers in Europe. Despite 
that, the usage of plant species in traditional medicine of some Balkan regions remained 
largely unexplored in the past. This study aimed to collect and document data on the 
traditional use of medicinal plants in Pčinja district in South-Eastern Serbia, which is 
among the least developed regions in Serbia. The survey was carried out using semi structured interviews and 113 informants were interviewed. Quantitative ethnobotany 
factors were calculated, allowing us discussing the results.
The informants reported data on 86 medicinal plants belonging to 43 families. 
The most dominant botanical families were Lamiaceae (30% of species), Asteraceae 
(26% of species) and Rosaceae (19% of species). Species with the highest number of 
use reports were Mentha piperita, Hypericum perforatum, Matricaria chamomilla, Salvia
officinalis, Urtica dioica, Thymus serpyllum and Tilia cordata. The majority of the plants 
were reported to have 1-3 different usages and the species with most diverse uses were 
Hypericum perforatum and Urtica dioica. In Pčinja district folk medicine is primarily used 
for healing minor diseases with some exceptions. The most frequent medicinal uses 
were for treating diseases of the digestive system, respiratory system and diseases of 
the skin and subcutaneous tissue, followed by general and unspecified diseases (such 
as pain, fever and health prevention). Usually, the administration was primarily oral 
followed by topical applications. Leaves were dominantly exploited plant parts and 
the most frequent preparation form was infusion. The current study represents a useful 
documentation which can contribute to preserving ethnobotanical knowledge in 
South-Eastern Serbia. For some well-known plant species new applications have been 
recorded. This can present a good starting point for new investigations",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade",
journal = "The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia",
title = "Ethnobotanical study of medicinal plants traditionally used in South-East Serbia (Pčinja district)",
pages = "28",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4371"
}

Živković, J., Ilić, M., Stojković, D.,& Šavikin, K.. (2019). Ethnobotanical study of medicinal plants traditionally used in South-East Serbia (Pčinja district). in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade., 28.
https://hdl.handle.net/21.15107/rcub_ibiss_4371

Živković J, Ilić M, Stojković D, Šavikin K. Ethnobotanical study of medicinal plants traditionally used in South-East Serbia (Pčinja district). in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia. 2019;:28.
https://hdl.handle.net/21.15107/rcub_ibiss_4371 .

Živković, Jelena, Ilić, Milan, Stojković, Dejan, Šavikin, Katarina, "Ethnobotanical study of medicinal plants traditionally used in South-East Serbia (Pčinja district)" in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia (2019):28,
https://hdl.handle.net/21.15107/rcub_ibiss_4371 .

TY  - CONF
AU  - Mićanović, Dragica
AU  - Saksida, Tamara
AU  - Koprivica, Ivan
AU  - Vujičić, Milica
AU  - Despotović, Sanja
AU  - Šavikin, Katarina
AU  - Janković, Teodora
AU  - Stojanović, Ivana D.
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5777
AB  - Chokeberry (Aronia melanocarpa) is known for its strong anti-oxidant properties. Antiinflammatory,
anti-hypertensive and anti-diabetogenic activities of orally consumed
chokeberry extracts have also been reported. The effects of chokeberry extract on the
immune response parameters have been only sporadically assessed. Therefore, the aim of
our study was to investigate the effects of orally consumed chokeberry extract on the
immune response in vivo and in vitro in healthy and in diabetic C57BL/6 mice, in which
diabetes was induced by multiple low doses of streptozotocin (MLDS). Chokeberry extract
administered to healthy mice (50 mg/kg body weight) exerted immunomodulatory effects
as evidenced by decreased proportion of F4/80+ macrophages, CD11c+ dendritic cells,
CD4+ T helper cells, CD8+ T cytotoxic lymphocytes and CD4+CD25- activated T
lymphocytes within the gut-associated lymphoid tissue. Surprisingly, oral consumption of
chokeberry extract in doses of either 200 mg/kg bw or 50 mg/kg bw in diabetic mice
resulted in the increase of blood glucose levels. In an attempt to decipher the underlying
mechanisms of chokeberry extract effects in the context of autoimmune/inflammatory
disease, we have evaluated its effects in vitro on purified immune cells. Seemingly, the
chokeberry extract exerted pro-inflammatory effects in vitro through the up-regulation of
nitric oxide and IL-1β production in macrophages and dendritic cells, increased
macrophage CD86-related activation and promotion of type 1 T helper cells (IFN-γ+)
differentiation. In addition, an increased proportion of CD4+, CD8+ and B lymphocytes
within the spleen was observed. Collectively, the obtained results imply that our particular
chokeberry extract displays pro-inflammatory characteristics and that care should be taken
when chokeberry is to be included in the human diet.
PB  - Belgrade: Faculty of Chemistry
C3  - The 9th Conference of the Serbian Biochemical Society: Diversity in Biochemistry; 2019 Nov 14-16; Belgrade, Serbia.
T1  - Chokeberry (Aronia melanocarpa) fruit extract modulates mouse immune response in vivo and in vitro
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5777
ER  - 

@conference{
author = "Mićanović, Dragica and Saksida, Tamara and Koprivica, Ivan and Vujičić, Milica and Despotović, Sanja and Šavikin, Katarina and Janković, Teodora and Stojanović, Ivana D.",
year = "2019",
abstract = "Chokeberry (Aronia melanocarpa) is known for its strong anti-oxidant properties. Antiinflammatory,
anti-hypertensive and anti-diabetogenic activities of orally consumed
chokeberry extracts have also been reported. The effects of chokeberry extract on the
immune response parameters have been only sporadically assessed. Therefore, the aim of
our study was to investigate the effects of orally consumed chokeberry extract on the
immune response in vivo and in vitro in healthy and in diabetic C57BL/6 mice, in which
diabetes was induced by multiple low doses of streptozotocin (MLDS). Chokeberry extract
administered to healthy mice (50 mg/kg body weight) exerted immunomodulatory effects
as evidenced by decreased proportion of F4/80+ macrophages, CD11c+ dendritic cells,
CD4+ T helper cells, CD8+ T cytotoxic lymphocytes and CD4+CD25- activated T
lymphocytes within the gut-associated lymphoid tissue. Surprisingly, oral consumption of
chokeberry extract in doses of either 200 mg/kg bw or 50 mg/kg bw in diabetic mice
resulted in the increase of blood glucose levels. In an attempt to decipher the underlying
mechanisms of chokeberry extract effects in the context of autoimmune/inflammatory
disease, we have evaluated its effects in vitro on purified immune cells. Seemingly, the
chokeberry extract exerted pro-inflammatory effects in vitro through the up-regulation of
nitric oxide and IL-1β production in macrophages and dendritic cells, increased
macrophage CD86-related activation and promotion of type 1 T helper cells (IFN-γ+)
differentiation. In addition, an increased proportion of CD4+, CD8+ and B lymphocytes
within the spleen was observed. Collectively, the obtained results imply that our particular
chokeberry extract displays pro-inflammatory characteristics and that care should be taken
when chokeberry is to be included in the human diet.",
publisher = "Belgrade: Faculty of Chemistry",
journal = "The 9th Conference of the Serbian Biochemical Society: Diversity in Biochemistry; 2019 Nov 14-16; Belgrade, Serbia.",
title = "Chokeberry (Aronia melanocarpa) fruit extract modulates mouse immune response in vivo and in vitro",
pages = "96",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5777"
}

Mićanović, D., Saksida, T., Koprivica, I., Vujičić, M., Despotović, S., Šavikin, K., Janković, T.,& Stojanović, I. D.. (2019). Chokeberry (Aronia melanocarpa) fruit extract modulates mouse immune response in vivo and in vitro. in The 9th Conference of the Serbian Biochemical Society: Diversity in Biochemistry; 2019 Nov 14-16; Belgrade, Serbia.
Belgrade: Faculty of Chemistry., 96.
https://hdl.handle.net/21.15107/rcub_ibiss_5777

Mićanović D, Saksida T, Koprivica I, Vujičić M, Despotović S, Šavikin K, Janković T, Stojanović ID. Chokeberry (Aronia melanocarpa) fruit extract modulates mouse immune response in vivo and in vitro. in The 9th Conference of the Serbian Biochemical Society: Diversity in Biochemistry; 2019 Nov 14-16; Belgrade, Serbia.. 2019;:96.
https://hdl.handle.net/21.15107/rcub_ibiss_5777 .

Mićanović, Dragica, Saksida, Tamara, Koprivica, Ivan, Vujičić, Milica, Despotović, Sanja, Šavikin, Katarina, Janković, Teodora, Stojanović, Ivana D., "Chokeberry (Aronia melanocarpa) fruit extract modulates mouse immune response in vivo and in vitro" in The 9th Conference of the Serbian Biochemical Society: Diversity in Biochemistry; 2019 Nov 14-16; Belgrade, Serbia. (2019):96,
https://hdl.handle.net/21.15107/rcub_ibiss_5777 .

TY  - JOUR
AU  - Stojanović, Ivana D.
AU  - Šavikin, Katarina
AU  - Nikolovski, Neda
AU  - Živković, Jelena
AU  - Saksida, Tamara
AU  - Momčilović, Miljana
AU  - Koprivica, Ivan
AU  - Vujičić, Milica
AU  - Stanisavljević, Suzana
AU  - Miljković, Đorđe
AU  - Menković, Nebojša
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S1756464617303353
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2793
AB  - Pomegranate peel extract (PPE) was obtained by aqueous-ethanol extraction and was abundant in punicalin, punicalagin and ellagic acid. As these compounds are known to act against inflammation and oxidative stress in synergistic fashion, effects of PPE were tested in vitro on immune cells and in animal models of multiple sclerosis and type 1 diabetes (T1D). In vitro, PPE inhibited interleukin-17 (IL-17) production from gut-associated lymphoid tissue (GALT) cells. PPE also reduced production of IL-17 in activated T cells isolated from animals with experimental autoimmune encephalomyelitis (EAE). It efficiently down-regulated clinical symptoms of EAE in DA rats and of streptozotocin-induced T1D in C57BL/6 mice. The effect of PPE in T1D was mediated by inhibition of immune cell infiltration into pancreatic islets and through interference with IL-17 and IFN-γ production in GALT. Our results suggest that PPE has the potential to be used as phytopharmaceutical for certain autoimmune and chronic inflammatory disorders.
T2  - Journal of Functional Foods
T1  - Pomegranate peel extract ameliorates autoimmunity in animal models of multiple sclerosis and type 1 diabetes
VL  - 35
DO  - 10.1016/j.jff.2017.06.021
SP  - 522
EP  - 530
ER  - 

@article{
author = "Stojanović, Ivana D. and Šavikin, Katarina and Nikolovski, Neda and Živković, Jelena and Saksida, Tamara and Momčilović, Miljana and Koprivica, Ivan and Vujičić, Milica and Stanisavljević, Suzana and Miljković, Đorđe and Menković, Nebojša",
year = "2017",
abstract = "Pomegranate peel extract (PPE) was obtained by aqueous-ethanol extraction and was abundant in punicalin, punicalagin and ellagic acid. As these compounds are known to act against inflammation and oxidative stress in synergistic fashion, effects of PPE were tested in vitro on immune cells and in animal models of multiple sclerosis and type 1 diabetes (T1D). In vitro, PPE inhibited interleukin-17 (IL-17) production from gut-associated lymphoid tissue (GALT) cells. PPE also reduced production of IL-17 in activated T cells isolated from animals with experimental autoimmune encephalomyelitis (EAE). It efficiently down-regulated clinical symptoms of EAE in DA rats and of streptozotocin-induced T1D in C57BL/6 mice. The effect of PPE in T1D was mediated by inhibition of immune cell infiltration into pancreatic islets and through interference with IL-17 and IFN-γ production in GALT. Our results suggest that PPE has the potential to be used as phytopharmaceutical for certain autoimmune and chronic inflammatory disorders.",
journal = "Journal of Functional Foods",
title = "Pomegranate peel extract ameliorates autoimmunity in animal models of multiple sclerosis and type 1 diabetes",
volume = "35",
doi = "10.1016/j.jff.2017.06.021",
pages = "522-530"
}

Stojanović, I. D., Šavikin, K., Nikolovski, N., Živković, J., Saksida, T., Momčilović, M., Koprivica, I., Vujičić, M., Stanisavljević, S., Miljković, Đ.,& Menković, N.. (2017). Pomegranate peel extract ameliorates autoimmunity in animal models of multiple sclerosis and type 1 diabetes. in Journal of Functional Foods, 35, 522-530.
https://doi.org/10.1016/j.jff.2017.06.021

Stojanović ID, Šavikin K, Nikolovski N, Živković J, Saksida T, Momčilović M, Koprivica I, Vujičić M, Stanisavljević S, Miljković Đ, Menković N. Pomegranate peel extract ameliorates autoimmunity in animal models of multiple sclerosis and type 1 diabetes. in Journal of Functional Foods. 2017;35:522-530.
doi:10.1016/j.jff.2017.06.021 .

Stojanović, Ivana D., Šavikin, Katarina, Nikolovski, Neda, Živković, Jelena, Saksida, Tamara, Momčilović, Miljana, Koprivica, Ivan, Vujičić, Milica, Stanisavljević, Suzana, Miljković, Đorđe, Menković, Nebojša, "Pomegranate peel extract ameliorates autoimmunity in animal models of multiple sclerosis and type 1 diabetes" in Journal of Functional Foods, 35 (2017):522-530,
https://doi.org/10.1016/j.jff.2017.06.021 . .

TY  - JOUR
AU  - Stević, Tatjana
AU  - Stanković, Slaviša
AU  - Šavikin, Katarina
AU  - Gođevac, Dejan
AU  - Dimkić, Ivica
AU  - Soković, Marina
AU  - Berić, Tanja
PY  - 2014
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/412
AB  - In recent years great attention was paid to biological control, the application of natural products in order to protect crops and medicinal plants against contamination with phytopathogenic and saprophytic fungi. Essential oils have been evaluated as a potentially safe replacement for chemicals used for that purpose. In this regard, antifungal potential of six essential oils was examined coupled with determination of the complete qualitative and quantitative chemical composition by GC-FID and GC-MS analysis. While essential oils of rosemary, sage, Spanish sage and black pepper were exclusively composed of monoterpenes, vetiver essential oil was entirely composed of sesquiterpenes. In essential oil of cinnamon dominates (E)-cinnamaldehyde. Applying the in vitro microdilution method, it was found that all essential oils were active in inhibiting the growth of all tested 21 pre- and post-harvest phytopathogenic and saprophytic fungi. MIC and MFC ranged from 1.2 mg ml-1 up to 22.6 mg ml-1 according to the test oil. Rosemary oil showed the best antifungal potential, followed by black pepper and cinnamon oil. Sage and Spanish sage oils also exhibited significant antifungal potential. Vetiver oil demonstrated the lowest antifungal activity. Essential oils that showed considerable antifungal potential are good candidates for further examination of their use in preventing and/or protection of medicinal plants, their seeds and dried drugs against fungal infections, both in the field and in warehouses.
AB  - Poslednjih godina velika pažnja poklanja se biološkoj kontroli to jest primeni prirodnih produkata u zaštiti, kako poljoprivrednih kultura, tako i lekovitog bilja od kontaminacije fitopatogenim i saprofitnim gljivama. U tom smislu, ispitivan je antifungalni potencijal šest etarskih ulja uz određivanje kompletnog kvalitativnog i kvantitativnog hemijskog sastava primenom GC-FID i GC-MS analiza. Dok se korišćeni uzorci etarskih ulja ruzmarina, žalfije, španske žalfije i crnog bibera isključivo sastoje od monoterpena, u ulju vetivera potpuno dominiraju seskviterpeni. U etarskom ulju cimeta najzastupljenija komponneta je bila (E)- cinemaldehid. Primenom in vitro mikrodilucione metode, utvrđeno je da su sva ulja bila aktivna u inhibiciji rasta 21 testirane, pre- i post-žetvene, fitopatogene i saprofitne gljive, sa razlikama u efikasnosti. MIC i MFC vrednosti su varirale u opsegu od 1,2 mg ml-1 do 22,6 mg ml-1, u zavisnosti od ispitivanog ulja. Najbolja antifungalna aktivnost utvrđena je za ulje ruzmarina, nešto slabija za ulja crnog bibera i cimeta, dok je ulje vetivera ispoljilo najslabiju aktivnost. Etarska ulja obe vrste žalfija ispoljila su značajan antifungalni potencijal. Etarska ulja koja poseduju značajan antifungalni potencijal su dobri kandidati za nastavak istraživanja u pravcu njihove primene u prevenciji i zaštiti useva lekovitog bilja, kao i njihovih semena i osušenih biljnih droga od gljivičnih infekcija, i u polju i u skladištima.
T2  - Lekovite sirovine
T1  - Hemijski sastav i inhibitorna aktivnost odabranih etarskih ulja na gljive izolovane sa lekovitog bilja
T1  - Chemical composition and inhibitory activity of selected essential oils against fungi isolated from medicinal plants
IS  - 34
VL  - 34
SP  - 69
EP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_412
ER  - 

@article{
author = "Stević, Tatjana and Stanković, Slaviša and Šavikin, Katarina and Gođevac, Dejan and Dimkić, Ivica and Soković, Marina and Berić, Tanja",
year = "2014, 2014",
abstract = "In recent years great attention was paid to biological control, the application of natural products in order to protect crops and medicinal plants against contamination with phytopathogenic and saprophytic fungi. Essential oils have been evaluated as a potentially safe replacement for chemicals used for that purpose. In this regard, antifungal potential of six essential oils was examined coupled with determination of the complete qualitative and quantitative chemical composition by GC-FID and GC-MS analysis. While essential oils of rosemary, sage, Spanish sage and black pepper were exclusively composed of monoterpenes, vetiver essential oil was entirely composed of sesquiterpenes. In essential oil of cinnamon dominates (E)-cinnamaldehyde. Applying the in vitro microdilution method, it was found that all essential oils were active in inhibiting the growth of all tested 21 pre- and post-harvest phytopathogenic and saprophytic fungi. MIC and MFC ranged from 1.2 mg ml-1 up to 22.6 mg ml-1 according to the test oil. Rosemary oil showed the best antifungal potential, followed by black pepper and cinnamon oil. Sage and Spanish sage oils also exhibited significant antifungal potential. Vetiver oil demonstrated the lowest antifungal activity. Essential oils that showed considerable antifungal potential are good candidates for further examination of their use in preventing and/or protection of medicinal plants, their seeds and dried drugs against fungal infections, both in the field and in warehouses., Poslednjih godina velika pažnja poklanja se biološkoj kontroli to jest primeni prirodnih produkata u zaštiti, kako poljoprivrednih kultura, tako i lekovitog bilja od kontaminacije fitopatogenim i saprofitnim gljivama. U tom smislu, ispitivan je antifungalni potencijal šest etarskih ulja uz određivanje kompletnog kvalitativnog i kvantitativnog hemijskog sastava primenom GC-FID i GC-MS analiza. Dok se korišćeni uzorci etarskih ulja ruzmarina, žalfije, španske žalfije i crnog bibera isključivo sastoje od monoterpena, u ulju vetivera potpuno dominiraju seskviterpeni. U etarskom ulju cimeta najzastupljenija komponneta je bila (E)- cinemaldehid. Primenom in vitro mikrodilucione metode, utvrđeno je da su sva ulja bila aktivna u inhibiciji rasta 21 testirane, pre- i post-žetvene, fitopatogene i saprofitne gljive, sa razlikama u efikasnosti. MIC i MFC vrednosti su varirale u opsegu od 1,2 mg ml-1 do 22,6 mg ml-1, u zavisnosti od ispitivanog ulja. Najbolja antifungalna aktivnost utvrđena je za ulje ruzmarina, nešto slabija za ulja crnog bibera i cimeta, dok je ulje vetivera ispoljilo najslabiju aktivnost. Etarska ulja obe vrste žalfija ispoljila su značajan antifungalni potencijal. Etarska ulja koja poseduju značajan antifungalni potencijal su dobri kandidati za nastavak istraživanja u pravcu njihove primene u prevenciji i zaštiti useva lekovitog bilja, kao i njihovih semena i osušenih biljnih droga od gljivičnih infekcija, i u polju i u skladištima.",
journal = "Lekovite sirovine",
title = "Hemijski sastav i inhibitorna aktivnost odabranih etarskih ulja na gljive izolovane sa lekovitog bilja, Chemical composition and inhibitory activity of selected essential oils against fungi isolated from medicinal plants",
number = "34",
volume = "34",
pages = "69-80",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_412"
}

Stević, T., Stanković, S., Šavikin, K., Gođevac, D., Dimkić, I., Soković, M.,& Berić, T.. (2014). Hemijski sastav i inhibitorna aktivnost odabranih etarskih ulja na gljive izolovane sa lekovitog bilja. in Lekovite sirovine, 34(34), 69-80.
https://hdl.handle.net/21.15107/rcub_ibiss_412

Stević T, Stanković S, Šavikin K, Gođevac D, Dimkić I, Soković M, Berić T. Hemijski sastav i inhibitorna aktivnost odabranih etarskih ulja na gljive izolovane sa lekovitog bilja. in Lekovite sirovine. 2014;34(34):69-80.
https://hdl.handle.net/21.15107/rcub_ibiss_412 .

Stević, Tatjana, Stanković, Slaviša, Šavikin, Katarina, Gođevac, Dejan, Dimkić, Ivica, Soković, Marina, Berić, Tanja, "Hemijski sastav i inhibitorna aktivnost odabranih etarskih ulja na gljive izolovane sa lekovitog bilja" in Lekovite sirovine, 34, no. 34 (2014):69-80,
https://hdl.handle.net/21.15107/rcub_ibiss_412 .