@article{
author = "Tepavcevic, Snezana and Vojnović-Milutinović, Danijela and Macut, Djuro and Zakula, Zorica and Radovanović, Marina and Bozic-Antic, Ivana and Romic, Snjezana and Bjekić-Macut, Jelica and Matić, Gordana and Koricanac, Goran",
year = "2014",
abstract = "It is supposed that women with polycystic ovary syndrome (PCOS) are
prone to develop cardiovascular disease as a consequence of multiple
risk factors that are mostly related to the state of insulin resistance
and consequent hyperinsulinemia. In the present study, we evaluated
insulin signaling and glucose transporters (GLUT) in cardiac cells of
dihydrotestosterone (DHT) treated female rats as an animal model of
PCOS. Expression of proteins involved in cardiac insulin signaling
pathways and glucose transporters, as well as their phosphorylation or
intracellular localization were studied by Western blot analysis in
DHT-treated and control rats. Treatment with DHT resulted in increased
body mass, absolute mass of the heart, elevated plasma insulin
concentration, dyslipidemia and insulin resistance. At the molecular
level, DHT treatment did not change protein expression of cardiac
insulin receptor and insulin receptor substrate 1, while phosphorylation
of the substrate at serine 307 was increased. Unexpectedly, although
expression of downstream Akt kinase and its phosphorylation at threonine
308 were not altered, phosphoiylation of Akt at serine 473 was increased
in the heart of DHT-treated rats. In contrast, expression and
phosphorylation of extracellular signal regulated kinases 1/2 were
decreased. Plasma membrane contents of GLUT1 and GLUT4 were decreased,
as well as the expression of GLUT4 in cardiac cells at the end of
androgen treatment. The obtained results provide evidence for
alterations in expression and especially in functional characteristics
of insulin signaling molecules and glucose transporters in the heart of
DHT-treated rats with PCOS, indicating impaired cardiac insulin action.
(c) 2014 Elsevier Ltd. All rights reserved.",
journal = "Journal of Steroid Biochemistry and Molecular Biology",
title = "Dihydrotestosterone deteriorates cardiac insulin signaling and glucose
transport in the rat model of polycystic ovary syndrome",
volume = "141",
doi = "10.1016/j.jsbmb.2014.01.006",
pages = "71-76"
}
