TY  - JOUR
AU  - Tepavcevic, Snezana
AU  - Vojnović-Milutinović, Danijela
AU  - Macut, Djuro
AU  - Stojiljkovic, Mojca
AU  - Radovanović, Marina
AU  - Bozic-Antic, Ivana
AU  - Culafic, Tijana
AU  - Bjekić-Macut, Jelica
AU  - Matić, Gordana
AU  - Koricanac, Goran
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1911
AB  - Polycystic ovary syndrome (PCOS) is associated with an altered plasma
   lipid profile and increased risk for cardiovascular diseases. We
   hypothesized that molecular mechanisms underlying cardiac pathology in
   PCOS involve changes in expression and subcellular localization of
   several key proteins involved in cardiac lipid transport and metabolism,
   such as fatty acid transporter CD36, lipin 1, peroxisome
   proliferator-activated receptor alpha (PPAR alpha), peroxisome
   proliferator-activated receptor gamma coactivator-1 (PGC1), and
   carnitine palmitoyltransferase 1 (CPT1). We used the animal model of
   PCOS obtained by treating female rats with dihydrotestosterone (DHT).
   Protein levels of CD36, lipin 1, PPAR alpha, PGC1, and antioxidative
   enzymes were assessed by Western blot in different cardiac cell
   compartments. Cardiac triglycerides (TG) and lipid peroxidation were
   also measured. The content of CD36 was decreased in both the cardiac
   plasma membranes and intracellular pool. On the other hand, total
   content of cardiac lipin 1 in DHT-treated rats was elevated, in contrast
   to decreased microsomal lipin 1 content. An increase in nuclear content
   of lipin 1 was observed together with elevation of nuclear PPAR alpha
   and PGC1, and an increase in CPT1 expression. However, lipid
   peroxidation was reduced in the heart, without alterations in
   antioxidative enzymes expression and cardiac TG content. The results
   indicate that treatment of female rats with DHT is accompanied by a
   decrease of fatty acid uptake and a reduction of lipid peroxidation in
   the heart. The observed elevation of lipin 1, PPAR alpha, PGC1, and CPT1
   expression suggests that cardiac fatty acid metabolism is shifted toward
   mitochondrial beta oxidation.
T2  - Endocrine
T1  - Cardiac fatty acid uptake and metabolism in the rat model of polycystic
 ovary syndrome
IS  - 1
VL  - 50
DO  - 10.1007/s12020-015-0558-1
SP  - 193
EP  - 201
ER  - 

@article{
author = "Tepavcevic, Snezana and Vojnović-Milutinović, Danijela and Macut, Djuro and Stojiljkovic, Mojca and Radovanović, Marina and Bozic-Antic, Ivana and Culafic, Tijana and Bjekić-Macut, Jelica and Matić, Gordana and Koricanac, Goran",
year = "2015",
abstract = "Polycystic ovary syndrome (PCOS) is associated with an altered plasma
   lipid profile and increased risk for cardiovascular diseases. We
   hypothesized that molecular mechanisms underlying cardiac pathology in
   PCOS involve changes in expression and subcellular localization of
   several key proteins involved in cardiac lipid transport and metabolism,
   such as fatty acid transporter CD36, lipin 1, peroxisome
   proliferator-activated receptor alpha (PPAR alpha), peroxisome
   proliferator-activated receptor gamma coactivator-1 (PGC1), and
   carnitine palmitoyltransferase 1 (CPT1). We used the animal model of
   PCOS obtained by treating female rats with dihydrotestosterone (DHT).
   Protein levels of CD36, lipin 1, PPAR alpha, PGC1, and antioxidative
   enzymes were assessed by Western blot in different cardiac cell
   compartments. Cardiac triglycerides (TG) and lipid peroxidation were
   also measured. The content of CD36 was decreased in both the cardiac
   plasma membranes and intracellular pool. On the other hand, total
   content of cardiac lipin 1 in DHT-treated rats was elevated, in contrast
   to decreased microsomal lipin 1 content. An increase in nuclear content
   of lipin 1 was observed together with elevation of nuclear PPAR alpha
   and PGC1, and an increase in CPT1 expression. However, lipid
   peroxidation was reduced in the heart, without alterations in
   antioxidative enzymes expression and cardiac TG content. The results
   indicate that treatment of female rats with DHT is accompanied by a
   decrease of fatty acid uptake and a reduction of lipid peroxidation in
   the heart. The observed elevation of lipin 1, PPAR alpha, PGC1, and CPT1
   expression suggests that cardiac fatty acid metabolism is shifted toward
   mitochondrial beta oxidation.",
journal = "Endocrine",
title = "Cardiac fatty acid uptake and metabolism in the rat model of polycystic
 ovary syndrome",
number = "1",
volume = "50",
doi = "10.1007/s12020-015-0558-1",
pages = "193-201"
}

Tepavcevic, S., Vojnović-Milutinović, D., Macut, D., Stojiljkovic, M., Radovanović, M., Bozic-Antic, I., Culafic, T., Bjekić-Macut, J., Matić, G.,& Koricanac, G.. (2015). Cardiac fatty acid uptake and metabolism in the rat model of polycystic
 ovary syndrome. in Endocrine, 50(1), 193-201.
https://doi.org/10.1007/s12020-015-0558-1

Tepavcevic S, Vojnović-Milutinović D, Macut D, Stojiljkovic M, Radovanović M, Bozic-Antic I, Culafic T, Bjekić-Macut J, Matić G, Koricanac G. Cardiac fatty acid uptake and metabolism in the rat model of polycystic
 ovary syndrome. in Endocrine. 2015;50(1):193-201.
doi:10.1007/s12020-015-0558-1 .

Tepavcevic, Snezana, Vojnović-Milutinović, Danijela, Macut, Djuro, Stojiljkovic, Mojca, Radovanović, Marina, Bozic-Antic, Ivana, Culafic, Tijana, Bjekić-Macut, Jelica, Matić, Gordana, Koricanac, Goran, "Cardiac fatty acid uptake and metabolism in the rat model of polycystic
 ovary syndrome" in Endocrine, 50, no. 1 (2015):193-201,
https://doi.org/10.1007/s12020-015-0558-1 . .

TY  - JOUR
AU  - Radovanović, Marina
AU  - Macut, Đuro
AU  - Đorđević, Ana
AU  - Veličković, Nataša
AU  - Nestorović, Nataša
AU  - Bursać, Biljana
AU  - Božić-Antić, Ivana
AU  - Bjekić Macut, Jelica
AU  - Matić, Gordana
AU  - Vojnović-Milutinović, Danijela
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2022
UR  - https://www.sciencedirect.com/science/article/pii/S0303720714002706?via%3Dihub#ac0010
AB  - Polycystic ovary syndrome (PCOS) is a reproductive and metabolic
   disorder characterized by hyperandrogenism, ovulatory dysfunction,
   visceral obesity and insulin resistance. We hypothesized that changes in
   glucocorticoid metabolism and signaling in the visceral adipose tissue
   may contribute to disturbances of lipid metabolism in the rat model of
   PCOS obtained by 5 alpha-dihydrotestosterone (DHT) treatment of
   prepubertal female Wistar rats. The results confirmed that DHT treatment
   caused anovulation, obesity and dyslipidemia. Enhanced glucocorticoid
   prereceptor metabolism, assessed by elevated intracellular
   corticosterone and increased 11 beta-hydroxysteroid dehydrogenase type 1
   mRNA and protein levels, was accompanied by glucocorticoid receptor (GR)
   nuclear accumulation. In concert with the increased expression of
   GR-regulated prolipogenic genes (lipin-1, sterol regulatory element
   binding protein 1, fatty acid synthase, phosphoenolpyruvate
   carboxykinase), histological analyses revealed hypertrophic adipocytes.
   The results suggest that glucocorticoids influence lipid metabolism in
   the visceral adipose tissue in the way that may contribute to
   pathogenesis of metabolic disturbances associated with PCOS. (C) 2014
   Elsevier Ireland Ltd. All rights reserved.
T2  - Molecular and Cellular Endocrinology
T1  - Possible involvement of glucocorticoids in 5
 alpha-dihydrotestosterone-induced PCOS-like metabolic disturbances in
 the rat visceral adipose tissue
IS  - C
VL  - 399
DO  - 10.1016/j.mce.2014.08.013
SP  - 22
EP  - 31
ER  - 

@article{
author = "Radovanović, Marina and Macut, Đuro and Đorđević, Ana and Veličković, Nataša and Nestorović, Nataša and Bursać, Biljana and Božić-Antić, Ivana and Bjekić Macut, Jelica and Matić, Gordana and Vojnović-Milutinović, Danijela",
year = "2015",
abstract = "Polycystic ovary syndrome (PCOS) is a reproductive and metabolic
   disorder characterized by hyperandrogenism, ovulatory dysfunction,
   visceral obesity and insulin resistance. We hypothesized that changes in
   glucocorticoid metabolism and signaling in the visceral adipose tissue
   may contribute to disturbances of lipid metabolism in the rat model of
   PCOS obtained by 5 alpha-dihydrotestosterone (DHT) treatment of
   prepubertal female Wistar rats. The results confirmed that DHT treatment
   caused anovulation, obesity and dyslipidemia. Enhanced glucocorticoid
   prereceptor metabolism, assessed by elevated intracellular
   corticosterone and increased 11 beta-hydroxysteroid dehydrogenase type 1
   mRNA and protein levels, was accompanied by glucocorticoid receptor (GR)
   nuclear accumulation. In concert with the increased expression of
   GR-regulated prolipogenic genes (lipin-1, sterol regulatory element
   binding protein 1, fatty acid synthase, phosphoenolpyruvate
   carboxykinase), histological analyses revealed hypertrophic adipocytes.
   The results suggest that glucocorticoids influence lipid metabolism in
   the visceral adipose tissue in the way that may contribute to
   pathogenesis of metabolic disturbances associated with PCOS. (C) 2014
   Elsevier Ireland Ltd. All rights reserved.",
journal = "Molecular and Cellular Endocrinology",
title = "Possible involvement of glucocorticoids in 5
 alpha-dihydrotestosterone-induced PCOS-like metabolic disturbances in
 the rat visceral adipose tissue",
number = "C",
volume = "399",
doi = "10.1016/j.mce.2014.08.013",
pages = "22-31"
}

Radovanović, M., Macut, Đ., Đorđević, A., Veličković, N., Nestorović, N., Bursać, B., Božić-Antić, I., Bjekić Macut, J., Matić, G.,& Vojnović-Milutinović, D.. (2015). Possible involvement of glucocorticoids in 5
 alpha-dihydrotestosterone-induced PCOS-like metabolic disturbances in
 the rat visceral adipose tissue. in Molecular and Cellular Endocrinology, 399(C), 22-31.
https://doi.org/10.1016/j.mce.2014.08.013

Radovanović M, Macut Đ, Đorđević A, Veličković N, Nestorović N, Bursać B, Božić-Antić I, Bjekić Macut J, Matić G, Vojnović-Milutinović D. Possible involvement of glucocorticoids in 5
 alpha-dihydrotestosterone-induced PCOS-like metabolic disturbances in
 the rat visceral adipose tissue. in Molecular and Cellular Endocrinology. 2015;399(C):22-31.
doi:10.1016/j.mce.2014.08.013 .

Radovanović, Marina, Macut, Đuro, Đorđević, Ana, Veličković, Nataša, Nestorović, Nataša, Bursać, Biljana, Božić-Antić, Ivana, Bjekić Macut, Jelica, Matić, Gordana, Vojnović-Milutinović, Danijela, "Possible involvement of glucocorticoids in 5
 alpha-dihydrotestosterone-induced PCOS-like metabolic disturbances in
 the rat visceral adipose tissue" in Molecular and Cellular Endocrinology, 399, no. C (2015):22-31,
https://doi.org/10.1016/j.mce.2014.08.013 . .

TY  - JOUR
AU  - Macut, Djuro
AU  - Bozic-Antic, Ivana
AU  - Brkljačić, Jelena
AU  - Topalovic, Vladanka
AU  - Macut, Jelica Bjekic
AU  - Panidis, Dimitrios
AU  - Kotlica, Biljana Kastratovic
AU  - Papadakis, Efstathios
AU  - Matić, Gordana
AU  - Vojnović-Milutinović, Danijela
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2069
AB  - OBJECTIVE: Most women with PCOS have increased adrenal androgen
   production, enhanced peripheral metabolism of cortisol and elevation in
   urinary excretion of its metabolites. Increased cortisol clearance in
   PCOS is followed by a compensatory overdrive of the
   hypothalamic-pituitary-adrenocortical (HPA) axis. We hypothesized that
   oral contraceptives containing ethinylestradiol and drospirenone
   (EE-DRSP) could modulate glucocorticoid receptor (GR) expression and
   function and thus affect HPA axis activity in PCOS patients. DESIGN: We
   analyzed 12 women with PCOS (age 24.17 +/- 4.88 years; body mass index
   22.05 +/- 3.97 kg/m(2)) treated for 12 months with EE-DRSP and 20 BMI
   matched controls. In all subjects testosterone, dehydroepiandrosterone
   sulfate (DHEAS), sex hormone binding globulin (SHBG), cortisol (basal
   and after dexamethasone), concentrations of GR protein, phospo-GR211
   protein, number of GR per cell (B-max) and its equilibrium dissociation
   constant (K-D) were measured. RESULTS: Before treatment, increased
   concentrations of testosterone and DHEAS (p<0.001, respectively),
   unaltered basal cortisol and an increased sensitivity (p<0.05) of the
   HPA axis to dexamethasone were observed in PCOS women in comparison to
   controls. After treatment, testosterone (p<0.01), DHEAS (p<0.05) and
   cortisol suppression after dexamethasone (p<0.01) were decreased in PCOS
   women. There were no changes in GR protein concentration, GR
   phosphorylation nor in the receptor functional parameters B-max and K-D
   in women with PCOS before and after the therapy, and in comparison to
   controls. CONCLUSIONS: Prolonged treatment with EE-DRSP in PCOS women
   decreased serum androgens and increased cortisol in the presence of
   decreased sensitivity of the HPA axis and did not exert changes in GR
   expression and function.
T2  - Hormones-International Journal of Endocrinology and Metabolism
T1  - The influence of combined oral contraceptives containing drospirenone on
 hypothalamic-pituitary-adrenocortical axis activity and glucocorticoid
 receptor expression and function in women with polycystic ovary syndrome
IS  - 1
VL  - 14
SP  - 109
EP  - 117
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2069
ER  - 

@article{
author = "Macut, Djuro and Bozic-Antic, Ivana and Brkljačić, Jelena and Topalovic, Vladanka and Macut, Jelica Bjekic and Panidis, Dimitrios and Kotlica, Biljana Kastratovic and Papadakis, Efstathios and Matić, Gordana and Vojnović-Milutinović, Danijela",
year = "2015",
abstract = "OBJECTIVE: Most women with PCOS have increased adrenal androgen
   production, enhanced peripheral metabolism of cortisol and elevation in
   urinary excretion of its metabolites. Increased cortisol clearance in
   PCOS is followed by a compensatory overdrive of the
   hypothalamic-pituitary-adrenocortical (HPA) axis. We hypothesized that
   oral contraceptives containing ethinylestradiol and drospirenone
   (EE-DRSP) could modulate glucocorticoid receptor (GR) expression and
   function and thus affect HPA axis activity in PCOS patients. DESIGN: We
   analyzed 12 women with PCOS (age 24.17 +/- 4.88 years; body mass index
   22.05 +/- 3.97 kg/m(2)) treated for 12 months with EE-DRSP and 20 BMI
   matched controls. In all subjects testosterone, dehydroepiandrosterone
   sulfate (DHEAS), sex hormone binding globulin (SHBG), cortisol (basal
   and after dexamethasone), concentrations of GR protein, phospo-GR211
   protein, number of GR per cell (B-max) and its equilibrium dissociation
   constant (K-D) were measured. RESULTS: Before treatment, increased
   concentrations of testosterone and DHEAS (p<0.001, respectively),
   unaltered basal cortisol and an increased sensitivity (p<0.05) of the
   HPA axis to dexamethasone were observed in PCOS women in comparison to
   controls. After treatment, testosterone (p<0.01), DHEAS (p<0.05) and
   cortisol suppression after dexamethasone (p<0.01) were decreased in PCOS
   women. There were no changes in GR protein concentration, GR
   phosphorylation nor in the receptor functional parameters B-max and K-D
   in women with PCOS before and after the therapy, and in comparison to
   controls. CONCLUSIONS: Prolonged treatment with EE-DRSP in PCOS women
   decreased serum androgens and increased cortisol in the presence of
   decreased sensitivity of the HPA axis and did not exert changes in GR
   expression and function.",
journal = "Hormones-International Journal of Endocrinology and Metabolism",
title = "The influence of combined oral contraceptives containing drospirenone on
 hypothalamic-pituitary-adrenocortical axis activity and glucocorticoid
 receptor expression and function in women with polycystic ovary syndrome",
number = "1",
volume = "14",
pages = "109-117",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2069"
}

Macut, D., Bozic-Antic, I., Brkljačić, J., Topalovic, V., Macut, J. B., Panidis, D., Kotlica, B. K., Papadakis, E., Matić, G.,& Vojnović-Milutinović, D.. (2015). The influence of combined oral contraceptives containing drospirenone on
 hypothalamic-pituitary-adrenocortical axis activity and glucocorticoid
 receptor expression and function in women with polycystic ovary syndrome. in Hormones-International Journal of Endocrinology and Metabolism, 14(1), 109-117.
https://hdl.handle.net/21.15107/rcub_ibiss_2069

Macut D, Bozic-Antic I, Brkljačić J, Topalovic V, Macut JB, Panidis D, Kotlica BK, Papadakis E, Matić G, Vojnović-Milutinović D. The influence of combined oral contraceptives containing drospirenone on
 hypothalamic-pituitary-adrenocortical axis activity and glucocorticoid
 receptor expression and function in women with polycystic ovary syndrome. in Hormones-International Journal of Endocrinology and Metabolism. 2015;14(1):109-117.
https://hdl.handle.net/21.15107/rcub_ibiss_2069 .

Macut, Djuro, Bozic-Antic, Ivana, Brkljačić, Jelena, Topalovic, Vladanka, Macut, Jelica Bjekic, Panidis, Dimitrios, Kotlica, Biljana Kastratovic, Papadakis, Efstathios, Matić, Gordana, Vojnović-Milutinović, Danijela, "The influence of combined oral contraceptives containing drospirenone on
 hypothalamic-pituitary-adrenocortical axis activity and glucocorticoid
 receptor expression and function in women with polycystic ovary syndrome" in Hormones-International Journal of Endocrinology and Metabolism, 14, no. 1 (2015):109-117,
https://hdl.handle.net/21.15107/rcub_ibiss_2069 .

TY  - JOUR
AU  - Macut, Đuro
AU  - Bačević, Marina
AU  - Božić-Antić, Ivana
AU  - Bjekić-Macut, Jelica
AU  - Civcic, Milorad
AU  - Erceg, Snježana
AU  - Vojnović-Milutinović, Danijela
AU  - Stanojlović, Olivera
AU  - Andrić, Zoran
AU  - Kastratović-Kotlica, Biljana
AU  - Sukilović, Tijana
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2071
AB  - Background. Women with polycystic ovary syndrome (PCOS) could develop
   subclinical atherosclerosis during life. Purpose. To analyze
   cardiovascular risk (CVR) factors and their relation to clinical markers
   of cardiovascular disease (CVD) in respect to their age. Material and
   Methods. One hundred women with PCOS (26.32 +/- 5.26 years, BMI: 24.98
   +/- 6.38 kg/m(2)) were compared to 50 respective controls. In all
   subjects, total cholesterol (TC), HDL-C, LDL-C, triglycerides, TC/HDL-C
   and TG/HDL-C ratios, glucose, insulin and HOMA index, waist-to-hip ratio
   (WHR), systolic and diastolic blood pressure (SBP and DBP, resp.), and
   carotid intima-media thickness (CIMT) were analyzed in respect to their
   age and level of androgens. Results. PCOS over 30 years had higher WHR
   (P = 0.008), SBP (P < 0.001), DBP (P < 0.001), TC (P = 0.028), HDL-C (P
   = 0.028), LDL-C (P = 0.045), triglycerides (P < 0.001), TC/HDL-C (P <
   0.001), and triglycerides/HDL-C (P < 0.001) and had more prevalent
   hypertension and pronounced CIMT on common carotid arteries even after
   adjustment for BMI (P = 0.005 and 0.036, resp.). TC/ HDL-C and TG/HDL-C
   were higher in PCOS with the highest quintile of FAI in comparison to
   those with lower FAI (P = 0.045 and 0.034, resp.). Conclusions. PCOS
   women older than 30 years irrespective of BMI have the potential for
   early atherosclerosismirrored through the elevated lipids/lipid ratios
   and through changes in blood pressure.
T2  - International Journal of Endocrinology
T1  - Predictors of Subclinical Cardiovascular Disease in Women with
 Polycystic Ovary Syndrome: Interrelationship of Dyslipidemia and
 Arterial Blood Pressure
IS  - 812610
DO  - 10.1155/2015/812610
ER  - 

@article{
author = "Macut, Đuro and Bačević, Marina and Božić-Antić, Ivana and Bjekić-Macut, Jelica and Civcic, Milorad and Erceg, Snježana and Vojnović-Milutinović, Danijela and Stanojlović, Olivera and Andrić, Zoran and Kastratović-Kotlica, Biljana and Sukilović, Tijana",
year = "2015",
abstract = "Background. Women with polycystic ovary syndrome (PCOS) could develop
   subclinical atherosclerosis during life. Purpose. To analyze
   cardiovascular risk (CVR) factors and their relation to clinical markers
   of cardiovascular disease (CVD) in respect to their age. Material and
   Methods. One hundred women with PCOS (26.32 +/- 5.26 years, BMI: 24.98
   +/- 6.38 kg/m(2)) were compared to 50 respective controls. In all
   subjects, total cholesterol (TC), HDL-C, LDL-C, triglycerides, TC/HDL-C
   and TG/HDL-C ratios, glucose, insulin and HOMA index, waist-to-hip ratio
   (WHR), systolic and diastolic blood pressure (SBP and DBP, resp.), and
   carotid intima-media thickness (CIMT) were analyzed in respect to their
   age and level of androgens. Results. PCOS over 30 years had higher WHR
   (P = 0.008), SBP (P < 0.001), DBP (P < 0.001), TC (P = 0.028), HDL-C (P
   = 0.028), LDL-C (P = 0.045), triglycerides (P < 0.001), TC/HDL-C (P <
   0.001), and triglycerides/HDL-C (P < 0.001) and had more prevalent
   hypertension and pronounced CIMT on common carotid arteries even after
   adjustment for BMI (P = 0.005 and 0.036, resp.). TC/ HDL-C and TG/HDL-C
   were higher in PCOS with the highest quintile of FAI in comparison to
   those with lower FAI (P = 0.045 and 0.034, resp.). Conclusions. PCOS
   women older than 30 years irrespective of BMI have the potential for
   early atherosclerosismirrored through the elevated lipids/lipid ratios
   and through changes in blood pressure.",
journal = "International Journal of Endocrinology",
title = "Predictors of Subclinical Cardiovascular Disease in Women with
 Polycystic Ovary Syndrome: Interrelationship of Dyslipidemia and
 Arterial Blood Pressure",
number = "812610",
doi = "10.1155/2015/812610"
}

Macut, Đ., Bačević, M., Božić-Antić, I., Bjekić-Macut, J., Civcic, M., Erceg, S., Vojnović-Milutinović, D., Stanojlović, O., Andrić, Z., Kastratović-Kotlica, B.,& Sukilović, T.. (2015). Predictors of Subclinical Cardiovascular Disease in Women with
 Polycystic Ovary Syndrome: Interrelationship of Dyslipidemia and
 Arterial Blood Pressure. in International Journal of Endocrinology(812610).
https://doi.org/10.1155/2015/812610

Macut Đ, Bačević M, Božić-Antić I, Bjekić-Macut J, Civcic M, Erceg S, Vojnović-Milutinović D, Stanojlović O, Andrić Z, Kastratović-Kotlica B, Sukilović T. Predictors of Subclinical Cardiovascular Disease in Women with
 Polycystic Ovary Syndrome: Interrelationship of Dyslipidemia and
 Arterial Blood Pressure. in International Journal of Endocrinology. 2015;(812610).
doi:10.1155/2015/812610 .

Macut, Đuro, Bačević, Marina, Božić-Antić, Ivana, Bjekić-Macut, Jelica, Civcic, Milorad, Erceg, Snježana, Vojnović-Milutinović, Danijela, Stanojlović, Olivera, Andrić, Zoran, Kastratović-Kotlica, Biljana, Sukilović, Tijana, "Predictors of Subclinical Cardiovascular Disease in Women with
 Polycystic Ovary Syndrome: Interrelationship of Dyslipidemia and
 Arterial Blood Pressure" in International Journal of Endocrinology, no. 812610 (2015),
https://doi.org/10.1155/2015/812610 . .

TY  - JOUR
AU  - Tepavcevic, Snezana
AU  - Vojnović-Milutinović, Danijela
AU  - Macut, Djuro
AU  - Zakula, Zorica
AU  - Radovanović, Marina
AU  - Bozic-Antic, Ivana
AU  - Romic, Snjezana
AU  - Bjekić-Macut, Jelica
AU  - Matić, Gordana
AU  - Koricanac, Goran
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2217
AB  - It is supposed that women with polycystic ovary syndrome (PCOS) are
   prone to develop cardiovascular disease as a consequence of multiple
   risk factors that are mostly related to the state of insulin resistance
   and consequent hyperinsulinemia. In the present study, we evaluated
   insulin signaling and glucose transporters (GLUT) in cardiac cells of
   dihydrotestosterone (DHT) treated female rats as an animal model of
   PCOS. Expression of proteins involved in cardiac insulin signaling
   pathways and glucose transporters, as well as their phosphorylation or
   intracellular localization were studied by Western blot analysis in
   DHT-treated and control rats. Treatment with DHT resulted in increased
   body mass, absolute mass of the heart, elevated plasma insulin
   concentration, dyslipidemia and insulin resistance. At the molecular
   level, DHT treatment did not change protein expression of cardiac
   insulin receptor and insulin receptor substrate 1, while phosphorylation
   of the substrate at serine 307 was increased. Unexpectedly, although
   expression of downstream Akt kinase and its phosphorylation at threonine
   308 were not altered, phosphoiylation of Akt at serine 473 was increased
   in the heart of DHT-treated rats. In contrast, expression and
   phosphorylation of extracellular signal regulated kinases 1/2 were
   decreased. Plasma membrane contents of GLUT1 and GLUT4 were decreased,
   as well as the expression of GLUT4 in cardiac cells at the end of
   androgen treatment. The obtained results provide evidence for
   alterations in expression and especially in functional characteristics
   of insulin signaling molecules and glucose transporters in the heart of
   DHT-treated rats with PCOS, indicating impaired cardiac insulin action.
   (c) 2014 Elsevier Ltd. All rights reserved.
T2  - Journal of Steroid Biochemistry and Molecular Biology
T1  - Dihydrotestosterone deteriorates cardiac insulin signaling and glucose
 transport in the rat model of polycystic ovary syndrome
VL  - 141
DO  - 10.1016/j.jsbmb.2014.01.006
SP  - 71
EP  - 76
ER  - 

@article{
author = "Tepavcevic, Snezana and Vojnović-Milutinović, Danijela and Macut, Djuro and Zakula, Zorica and Radovanović, Marina and Bozic-Antic, Ivana and Romic, Snjezana and Bjekić-Macut, Jelica and Matić, Gordana and Koricanac, Goran",
year = "2014",
abstract = "It is supposed that women with polycystic ovary syndrome (PCOS) are
   prone to develop cardiovascular disease as a consequence of multiple
   risk factors that are mostly related to the state of insulin resistance
   and consequent hyperinsulinemia. In the present study, we evaluated
   insulin signaling and glucose transporters (GLUT) in cardiac cells of
   dihydrotestosterone (DHT) treated female rats as an animal model of
   PCOS. Expression of proteins involved in cardiac insulin signaling
   pathways and glucose transporters, as well as their phosphorylation or
   intracellular localization were studied by Western blot analysis in
   DHT-treated and control rats. Treatment with DHT resulted in increased
   body mass, absolute mass of the heart, elevated plasma insulin
   concentration, dyslipidemia and insulin resistance. At the molecular
   level, DHT treatment did not change protein expression of cardiac
   insulin receptor and insulin receptor substrate 1, while phosphorylation
   of the substrate at serine 307 was increased. Unexpectedly, although
   expression of downstream Akt kinase and its phosphorylation at threonine
   308 were not altered, phosphoiylation of Akt at serine 473 was increased
   in the heart of DHT-treated rats. In contrast, expression and
   phosphorylation of extracellular signal regulated kinases 1/2 were
   decreased. Plasma membrane contents of GLUT1 and GLUT4 were decreased,
   as well as the expression of GLUT4 in cardiac cells at the end of
   androgen treatment. The obtained results provide evidence for
   alterations in expression and especially in functional characteristics
   of insulin signaling molecules and glucose transporters in the heart of
   DHT-treated rats with PCOS, indicating impaired cardiac insulin action.
   (c) 2014 Elsevier Ltd. All rights reserved.",
journal = "Journal of Steroid Biochemistry and Molecular Biology",
title = "Dihydrotestosterone deteriorates cardiac insulin signaling and glucose
 transport in the rat model of polycystic ovary syndrome",
volume = "141",
doi = "10.1016/j.jsbmb.2014.01.006",
pages = "71-76"
}

Tepavcevic, S., Vojnović-Milutinović, D., Macut, D., Zakula, Z., Radovanović, M., Bozic-Antic, I., Romic, S., Bjekić-Macut, J., Matić, G.,& Koricanac, G.. (2014). Dihydrotestosterone deteriorates cardiac insulin signaling and glucose
 transport in the rat model of polycystic ovary syndrome. in Journal of Steroid Biochemistry and Molecular Biology, 141, 71-76.
https://doi.org/10.1016/j.jsbmb.2014.01.006

Tepavcevic S, Vojnović-Milutinović D, Macut D, Zakula Z, Radovanović M, Bozic-Antic I, Romic S, Bjekić-Macut J, Matić G, Koricanac G. Dihydrotestosterone deteriorates cardiac insulin signaling and glucose
 transport in the rat model of polycystic ovary syndrome. in Journal of Steroid Biochemistry and Molecular Biology. 2014;141:71-76.
doi:10.1016/j.jsbmb.2014.01.006 .

Tepavcevic, Snezana, Vojnović-Milutinović, Danijela, Macut, Djuro, Zakula, Zorica, Radovanović, Marina, Bozic-Antic, Ivana, Romic, Snjezana, Bjekić-Macut, Jelica, Matić, Gordana, Koricanac, Goran, "Dihydrotestosterone deteriorates cardiac insulin signaling and glucose
 transport in the rat model of polycystic ovary syndrome" in Journal of Steroid Biochemistry and Molecular Biology, 141 (2014):71-76,
https://doi.org/10.1016/j.jsbmb.2014.01.006 . .

TY  - JOUR
AU  - Vojnović-Milutinović, Danijela
AU  - Macut, Djuro
AU  - Bozic-Antic, Ivana
AU  - Macut, Jelica Bjekic
AU  - Radovanović, Marina
AU  - Matić, Gordana
AU  - Brkljačić, Jelena
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2157
AB  - Background: The polycystic ovary syndrome (PCOS) is a frequent endocrine
   disorder that affects women of reproductive age. As the syndrome is
   strongly associated with obesity, it is of interest to examine the gene
   expression differences that accompany its development and the associated
   metabolic disturbances. Real-time RT PCR is a standard method for
   studying changes in gene expression. However, to obtain accurate and
   reliable results, validation of reference genes is obligatory. The aim
   of this study was to identify a suitable reference for the normalization
   of gene expression in peripheral blood mononuclear cells (PBMCs) from
   obese and normal-weight women with PCOS.
   Methods: The expression stability of four potential reference genes:
   hypoxanthine guanine phosphoribosyl transferase 1 (HPRT), beta-actin
   (BA), beta(2)-microglobulin (B2M) and glyceraldehyde-3-phosphate
   dehydrogenase (GAPDH), was assessed in PBMCs from healthy women, and
   from normal-weight and obese women with PCOS. The variability in the
   expression of potential reference genes was analyzed by the TaqMan
   real-time RT PCR method, using GeNorm and Norm Finder software packages.
   Results: Direct comparison of cycle threshold (Ct) values showed
   inter-individual variations for all validated genes, the Ct values of
   HPRT being less variable than those of BA, GAPDH and B2M. Both software
   packages pointed to HPRT as the most steadily expressed gene in the
   PBMCs of women with PCOS and healthy controls.
   Conclusions: Cross-validation of the expression stability of four
   potential reference genes identified HPRT as the most stable reference,
   suitable for further investigations of gene expression in PBMCs from
   women with PCOS.
T2  - Journal of Medical Biochemistry
T1  - Hypoxanthine Guanine Phosphoribosyl Transferase Is the Most Stable Reference Gene for Gene Expression Analysis by Quantitative PCR in Peripheral Blood Mononuclear Cells from Women with the Polycystic Ovary Syndrome
IS  - 4
VL  - 33
DO  - 10.2478/jomb-2014-0029
SP  - 356
EP  - 363
ER  - 

@article{
author = "Vojnović-Milutinović, Danijela and Macut, Djuro and Bozic-Antic, Ivana and Macut, Jelica Bjekic and Radovanović, Marina and Matić, Gordana and Brkljačić, Jelena",
year = "2014",
abstract = "Background: The polycystic ovary syndrome (PCOS) is a frequent endocrine
   disorder that affects women of reproductive age. As the syndrome is
   strongly associated with obesity, it is of interest to examine the gene
   expression differences that accompany its development and the associated
   metabolic disturbances. Real-time RT PCR is a standard method for
   studying changes in gene expression. However, to obtain accurate and
   reliable results, validation of reference genes is obligatory. The aim
   of this study was to identify a suitable reference for the normalization
   of gene expression in peripheral blood mononuclear cells (PBMCs) from
   obese and normal-weight women with PCOS.
   Methods: The expression stability of four potential reference genes:
   hypoxanthine guanine phosphoribosyl transferase 1 (HPRT), beta-actin
   (BA), beta(2)-microglobulin (B2M) and glyceraldehyde-3-phosphate
   dehydrogenase (GAPDH), was assessed in PBMCs from healthy women, and
   from normal-weight and obese women with PCOS. The variability in the
   expression of potential reference genes was analyzed by the TaqMan
   real-time RT PCR method, using GeNorm and Norm Finder software packages.
   Results: Direct comparison of cycle threshold (Ct) values showed
   inter-individual variations for all validated genes, the Ct values of
   HPRT being less variable than those of BA, GAPDH and B2M. Both software
   packages pointed to HPRT as the most steadily expressed gene in the
   PBMCs of women with PCOS and healthy controls.
   Conclusions: Cross-validation of the expression stability of four
   potential reference genes identified HPRT as the most stable reference,
   suitable for further investigations of gene expression in PBMCs from
   women with PCOS.",
journal = "Journal of Medical Biochemistry",
title = "Hypoxanthine Guanine Phosphoribosyl Transferase Is the Most Stable Reference Gene for Gene Expression Analysis by Quantitative PCR in Peripheral Blood Mononuclear Cells from Women with the Polycystic Ovary Syndrome",
number = "4",
volume = "33",
doi = "10.2478/jomb-2014-0029",
pages = "356-363"
}

Vojnović-Milutinović, D., Macut, D., Bozic-Antic, I., Macut, J. B., Radovanović, M., Matić, G.,& Brkljačić, J.. (2014). Hypoxanthine Guanine Phosphoribosyl Transferase Is the Most Stable Reference Gene for Gene Expression Analysis by Quantitative PCR in Peripheral Blood Mononuclear Cells from Women with the Polycystic Ovary Syndrome. in Journal of Medical Biochemistry, 33(4), 356-363.
https://doi.org/10.2478/jomb-2014-0029

Vojnović-Milutinović D, Macut D, Bozic-Antic I, Macut JB, Radovanović M, Matić G, Brkljačić J. Hypoxanthine Guanine Phosphoribosyl Transferase Is the Most Stable Reference Gene for Gene Expression Analysis by Quantitative PCR in Peripheral Blood Mononuclear Cells from Women with the Polycystic Ovary Syndrome. in Journal of Medical Biochemistry. 2014;33(4):356-363.
doi:10.2478/jomb-2014-0029 .

Vojnović-Milutinović, Danijela, Macut, Djuro, Bozic-Antic, Ivana, Macut, Jelica Bjekic, Radovanović, Marina, Matić, Gordana, Brkljačić, Jelena, "Hypoxanthine Guanine Phosphoribosyl Transferase Is the Most Stable Reference Gene for Gene Expression Analysis by Quantitative PCR in Peripheral Blood Mononuclear Cells from Women with the Polycystic Ovary Syndrome" in Journal of Medical Biochemistry, 33, no. 4 (2014):356-363,
https://doi.org/10.2478/jomb-2014-0029 . .