TY  - JOUR
AU  - De Oliveira, Izamara
AU  - Chrysargyris, Antonios
AU  - Heleno, Sandrina A.
AU  - Carocho, Marcio
AU  - Calhelha, Ricardo
AU  - Dias, Maria Ines
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Petropoulos, Spyridon
AU  - Santos-Buelga, Celestino
AU  - Tzortzakis, Nikolaos
AU  - Barros, Lillian
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6389
AB  - The present study aims to determine the combined effect of cropping system and irrigation regime on the chemical composition and bioactive properties of lemon balm aerial parts. For this purpose, lemon balm plants were grown under two farming systems (conventional farming vs organic farming) and two irrigation levels (full irrigation vs deficit irrigation) and harvested twice throughout the growing period. The collected aerial parts were subjected to three different methods of extractions, namely infusion, maceration and ultrasound-assisted extraction and the obtained extracts were evaluated in terms of chemical profile and bioactivities. Five organic acids with varied composition among the tested treatments were identified in all the tested samples for both harvests, namely, citric, malic, oxalic, shikimic and quinic acid. Regarding phenolic compounds composition, the most abundant ones were rosmarinic acid, lithospermic acid A isomer I and hydroxylsalvianolic E, especially for the maceration and infusion extraction methods. Full irrigation resulted in lower EC50 values than deficit irrigation only in the treatments of the second harvest, while variable cytotoxic and anti-inflammatory effects were recorded in both harvests. Finally, in most cases the lemon balm extracts has similar or better activity than the positive controls, while the antifungal activity of lemon balm extracts was stronger than the antibacterial effects. In conclusion, the results of the present study showed that the implemented agronomic practices, as well as the extraction protocol may significantly affect the chemical profile and bioactivities of lemon balm extracts, suggesting that both the farming system and the irrigation schedule may improve the quality of the extracts depending on the implemented extraction protocol.
PB  - Elsevier
T2  - Food Research International
T1  - Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes.
VL  - 170
DO  - 10.1016/j.foodres.2023.113044
SP  - 113044
ER  - 

@article{
author = "De Oliveira, Izamara and Chrysargyris, Antonios and Heleno, Sandrina A. and Carocho, Marcio and Calhelha, Ricardo and Dias, Maria Ines and Petrović, Jovana and Soković, Marina and Petropoulos, Spyridon and Santos-Buelga, Celestino and Tzortzakis, Nikolaos and Barros, Lillian",
year = "2023",
abstract = "The present study aims to determine the combined effect of cropping system and irrigation regime on the chemical composition and bioactive properties of lemon balm aerial parts. For this purpose, lemon balm plants were grown under two farming systems (conventional farming vs organic farming) and two irrigation levels (full irrigation vs deficit irrigation) and harvested twice throughout the growing period. The collected aerial parts were subjected to three different methods of extractions, namely infusion, maceration and ultrasound-assisted extraction and the obtained extracts were evaluated in terms of chemical profile and bioactivities. Five organic acids with varied composition among the tested treatments were identified in all the tested samples for both harvests, namely, citric, malic, oxalic, shikimic and quinic acid. Regarding phenolic compounds composition, the most abundant ones were rosmarinic acid, lithospermic acid A isomer I and hydroxylsalvianolic E, especially for the maceration and infusion extraction methods. Full irrigation resulted in lower EC50 values than deficit irrigation only in the treatments of the second harvest, while variable cytotoxic and anti-inflammatory effects were recorded in both harvests. Finally, in most cases the lemon balm extracts has similar or better activity than the positive controls, while the antifungal activity of lemon balm extracts was stronger than the antibacterial effects. In conclusion, the results of the present study showed that the implemented agronomic practices, as well as the extraction protocol may significantly affect the chemical profile and bioactivities of lemon balm extracts, suggesting that both the farming system and the irrigation schedule may improve the quality of the extracts depending on the implemented extraction protocol.",
publisher = "Elsevier",
journal = "Food Research International",
title = "Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes.",
volume = "170",
doi = "10.1016/j.foodres.2023.113044",
pages = "113044"
}

De Oliveira, I., Chrysargyris, A., Heleno, S. A., Carocho, M., Calhelha, R., Dias, M. I., Petrović, J., Soković, M., Petropoulos, S., Santos-Buelga, C., Tzortzakis, N.,& Barros, L.. (2023). Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes.. in Food Research International
Elsevier., 170, 113044.
https://doi.org/10.1016/j.foodres.2023.113044

De Oliveira I, Chrysargyris A, Heleno SA, Carocho M, Calhelha R, Dias MI, Petrović J, Soković M, Petropoulos S, Santos-Buelga C, Tzortzakis N, Barros L. Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes.. in Food Research International. 2023;170:113044.
doi:10.1016/j.foodres.2023.113044 .

De Oliveira, Izamara, Chrysargyris, Antonios, Heleno, Sandrina A., Carocho, Marcio, Calhelha, Ricardo, Dias, Maria Ines, Petrović, Jovana, Soković, Marina, Petropoulos, Spyridon, Santos-Buelga, Celestino, Tzortzakis, Nikolaos, Barros, Lillian, "Effects of the extraction techniques on the chemical composition and bioactive properties of lemon balm (Melissa officinalis L.) plants grown under different cropping and irrigation regimes." in Food Research International, 170 (2023):113044,
https://doi.org/10.1016/j.foodres.2023.113044 . .

TY  - JOUR
AU  - Radovanović, Marina
AU  - Lazarević, Nevena
AU  - Novaković, Jovana
AU  - Jeremić, Nevena
AU  - Jakovljević, Vladimir
AU  - Živković, Vladimir
AU  - Bradić, Jovana
AU  - Pecarski, Danijela
AU  - Tel-Çayan, Gülsen
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Gregori, Andrej
AU  - Petrović, Jovana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5792
AB  - The present study aimed to examine the biological activity and cardioprotective potential of Trametes versicolor heteropolysaccharides (TVH) in a rat model of metabolic syndrome (MetS). This study included 40 Wistar rats divided into 5 groups: CTRL—healthy non-treated rats; MetS—non-treated rats; and H-TV, M-TV and L-TV-rats with MetS treated with either 300, 200 or 100 mg/kg TVH per os for 4 weeks. After finishing the treatment, we conducted an oral glucose tolerance test (OGTT), hemodynamic measurements and the animals were sacrificed, hearts isolated and subjected to the Langendorff technique. Blood samples were used for the determination of oxidative stress parameters, lipid status and insulin levels. We showed that α-amylase inhibition was not the mode of TVH antidiabetic action, while TVH showed a moderate inhibition of pathogenic microorganisms’ growth (MIC 8.00 mg·mL−1; MBC/MFC 16.00 mg·mL−1). H-TV and M-TV significantly reduced the level of prooxidants (O2−, H2O2, TBARS; p < 0.05), increased antioxidants activity (SOD, CAT, GSH; p < 0.05), reduced blood pressure (p < 0.05), improved glucose homeostasis in the OGTT test (p < 0.05), and ejection fraction (p < 0.05) and cardiac contractility (p < 0.05) compared to MetS (p < 0.05). Moreover, TVH treatment normalized the lipid status and decreased insulin levels compared to MetS rats (p < 0.05). The obtained results demonstrated that the TVH may be considered a useful agent for cardioprotection in MetS conditions.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome
IS  - 6
VL  - 16
DO  - 10.3390/ph16060787
SP  - 787
ER  - 

@article{
author = "Radovanović, Marina and Lazarević, Nevena and Novaković, Jovana and Jeremić, Nevena and Jakovljević, Vladimir and Živković, Vladimir and Bradić, Jovana and Pecarski, Danijela and Tel-Çayan, Gülsen and Glamočlija, Jasmina and Soković, Marina and Gregori, Andrej and Petrović, Jovana",
year = "2023",
abstract = "The present study aimed to examine the biological activity and cardioprotective potential of Trametes versicolor heteropolysaccharides (TVH) in a rat model of metabolic syndrome (MetS). This study included 40 Wistar rats divided into 5 groups: CTRL—healthy non-treated rats; MetS—non-treated rats; and H-TV, M-TV and L-TV-rats with MetS treated with either 300, 200 or 100 mg/kg TVH per os for 4 weeks. After finishing the treatment, we conducted an oral glucose tolerance test (OGTT), hemodynamic measurements and the animals were sacrificed, hearts isolated and subjected to the Langendorff technique. Blood samples were used for the determination of oxidative stress parameters, lipid status and insulin levels. We showed that α-amylase inhibition was not the mode of TVH antidiabetic action, while TVH showed a moderate inhibition of pathogenic microorganisms’ growth (MIC 8.00 mg·mL−1; MBC/MFC 16.00 mg·mL−1). H-TV and M-TV significantly reduced the level of prooxidants (O2−, H2O2, TBARS; p < 0.05), increased antioxidants activity (SOD, CAT, GSH; p < 0.05), reduced blood pressure (p < 0.05), improved glucose homeostasis in the OGTT test (p < 0.05), and ejection fraction (p < 0.05) and cardiac contractility (p < 0.05) compared to MetS (p < 0.05). Moreover, TVH treatment normalized the lipid status and decreased insulin levels compared to MetS rats (p < 0.05). The obtained results demonstrated that the TVH may be considered a useful agent for cardioprotection in MetS conditions.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome",
number = "6",
volume = "16",
doi = "10.3390/ph16060787",
pages = "787"
}

Radovanović, M., Lazarević, N., Novaković, J., Jeremić, N., Jakovljević, V., Živković, V., Bradić, J., Pecarski, D., Tel-Çayan, G., Glamočlija, J., Soković, M., Gregori, A.,& Petrović, J.. (2023). Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome. in Pharmaceuticals
Basel: MDPI., 16(6), 787.
https://doi.org/10.3390/ph16060787

Radovanović M, Lazarević N, Novaković J, Jeremić N, Jakovljević V, Živković V, Bradić J, Pecarski D, Tel-Çayan G, Glamočlija J, Soković M, Gregori A, Petrović J. Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome. in Pharmaceuticals. 2023;16(6):787.
doi:10.3390/ph16060787 .

Radovanović, Marina, Lazarević, Nevena, Novaković, Jovana, Jeremić, Nevena, Jakovljević, Vladimir, Živković, Vladimir, Bradić, Jovana, Pecarski, Danijela, Tel-Çayan, Gülsen, Glamočlija, Jasmina, Soković, Marina, Gregori, Andrej, Petrović, Jovana, "Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome" in Pharmaceuticals, 16, no. 6 (2023):787,
https://doi.org/10.3390/ph16060787 . .

TY  - JOUR
AU  - Albuquerque, Bianca R.
AU  - Pinela, José
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Calhelha, Ricardo C.
AU  - Oliveira, M Beatriz P P
AU  - Ferreira, Isabel C F R
AU  - Barros, Lillian
PY  - 2023
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0963996923001199
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5481
AB  - Chemical constituents and bioactive properties of rambutan (Nephelium lappaceum L.) peel were characterized and heat-/ultrasound-assisted extractions (HAE/UAE) of anthocyanins were optimized by response surface methodology. Five organic acids, the α-, γ-, and δ-tocopherol isoforms, and twenty-five fatty acids (36.8 % oleic acid) were identified, as well as a phenolic profile composed of ellagitannin derivatives, geraniin isomers, ellagic acid, and delphinidin-O derivatives. The extract showed antioxidant activity via lipid peroxidation (IC50 = 2.79 ± 0.03 µg/mL) and oxidative hemolysis (IC50 = 72 ± 2 µg/mL) inhibition, and displayed antibacterial and antifungal properties (MIC ≤ 1 mg/mL). On the other hand, no cytotoxicity was observed in tumor and non-tumor cell lines up to 400 µg/mL. The recovery of anthocyanins was more effective using HAE than UAE, allowing greater yields (16.2 mg/g extract) in just 3 min and using lower ethanol proportions. Overall, rambutan peel could be upcycled into bioactive ingredients and natural colorants for industrial applications.
T2  - Food Research International
T1  - Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins
VL  - 165
DO  - 10.1016/j.foodres.2023.112574
SP  - 112574
ER  - 

@article{
author = "Albuquerque, Bianca R. and Pinela, José and Dias, Maria Inês and Pereira, Carla and Petrović, Jovana and Soković, Marina and Calhelha, Ricardo C. and Oliveira, M Beatriz P P and Ferreira, Isabel C F R and Barros, Lillian",
year = "2023",
abstract = "Chemical constituents and bioactive properties of rambutan (Nephelium lappaceum L.) peel were characterized and heat-/ultrasound-assisted extractions (HAE/UAE) of anthocyanins were optimized by response surface methodology. Five organic acids, the α-, γ-, and δ-tocopherol isoforms, and twenty-five fatty acids (36.8 % oleic acid) were identified, as well as a phenolic profile composed of ellagitannin derivatives, geraniin isomers, ellagic acid, and delphinidin-O derivatives. The extract showed antioxidant activity via lipid peroxidation (IC50 = 2.79 ± 0.03 µg/mL) and oxidative hemolysis (IC50 = 72 ± 2 µg/mL) inhibition, and displayed antibacterial and antifungal properties (MIC ≤ 1 mg/mL). On the other hand, no cytotoxicity was observed in tumor and non-tumor cell lines up to 400 µg/mL. The recovery of anthocyanins was more effective using HAE than UAE, allowing greater yields (16.2 mg/g extract) in just 3 min and using lower ethanol proportions. Overall, rambutan peel could be upcycled into bioactive ingredients and natural colorants for industrial applications.",
journal = "Food Research International",
title = "Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins",
volume = "165",
doi = "10.1016/j.foodres.2023.112574",
pages = "112574"
}

Albuquerque, B. R., Pinela, J., Dias, M. I., Pereira, C., Petrović, J., Soković, M., Calhelha, R. C., Oliveira, M. B. P. P., Ferreira, I. C. F. R.,& Barros, L.. (2023). Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins. in Food Research International, 165, 112574.
https://doi.org/10.1016/j.foodres.2023.112574

Albuquerque BR, Pinela J, Dias MI, Pereira C, Petrović J, Soković M, Calhelha RC, Oliveira MBPP, Ferreira ICFR, Barros L. Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins. in Food Research International. 2023;165:112574.
doi:10.1016/j.foodres.2023.112574 .

Albuquerque, Bianca R., Pinela, José, Dias, Maria Inês, Pereira, Carla, Petrović, Jovana, Soković, Marina, Calhelha, Ricardo C., Oliveira, M Beatriz P P, Ferreira, Isabel C F R, Barros, Lillian, "Valorization of rambutan (Nephelium lappaceum L.) peel: Chemical composition, biological activity, and optimized recovery of anthocyanins" in Food Research International, 165 (2023):112574,
https://doi.org/10.1016/j.foodres.2023.112574 . .

TY  - JOUR
AU  - Popović Minić, Dušanka
AU  - Milinčić, Danijel
AU  - Kolašinac, Stefan
AU  - Rac, Vladislav
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Banjac, Nebojša
AU  - Lađarević, Jelena
AU  - Vidović, Bojana
AU  - Kostić, Aleksandar
AU  - Pavlović, Vladimir
AU  - Pešić, Mirjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5140
AB  - This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean particle diameter (from 194 nm to 225 nm). Fourier Transform Infrared Spectroscopy (FTIR) showed that ABM extracts provoked changes in the secondary structure of goat milk proteins and interactions between polysaccharides and milk proteins predominantly via hydrogen and/or glycoside bonds and hydrophobic interactions. The milk protein profiles revealed proteolytic activity in mixtures with AE resulting in the formation of five new polypeptides. The different microstructures of mixtures with AE and PE were found by Scanning Electron Microscopy (SEM). A schematic representation of possible milk proteins-ABM extracts interactions has been given.
PB  - Oxford: Elsevier
T2  - Food Chemistry
T1  - Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization
VL  - 402
DO  - 10.1016/j.foodchem.2022.134299
SP  - 134299
ER  - 

@article{
author = "Popović Minić, Dušanka and Milinčić, Danijel and Kolašinac, Stefan and Rac, Vladislav and Petrović, Jovana and Soković, Marina and Banjac, Nebojša and Lađarević, Jelena and Vidović, Bojana and Kostić, Aleksandar and Pavlović, Vladimir and Pešić, Mirjana",
year = "2023",
abstract = "This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean particle diameter (from 194 nm to 225 nm). Fourier Transform Infrared Spectroscopy (FTIR) showed that ABM extracts provoked changes in the secondary structure of goat milk proteins and interactions between polysaccharides and milk proteins predominantly via hydrogen and/or glycoside bonds and hydrophobic interactions. The milk protein profiles revealed proteolytic activity in mixtures with AE resulting in the formation of five new polypeptides. The different microstructures of mixtures with AE and PE were found by Scanning Electron Microscopy (SEM). A schematic representation of possible milk proteins-ABM extracts interactions has been given.",
publisher = "Oxford: Elsevier",
journal = "Food Chemistry",
title = "Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization",
volume = "402",
doi = "10.1016/j.foodchem.2022.134299",
pages = "134299"
}

Popović Minić, D., Milinčić, D., Kolašinac, S., Rac, V., Petrović, J., Soković, M., Banjac, N., Lađarević, J., Vidović, B., Kostić, A., Pavlović, V.,& Pešić, M.. (2023). Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization. in Food Chemistry
Oxford: Elsevier., 402, 134299.
https://doi.org/10.1016/j.foodchem.2022.134299

Popović Minić D, Milinčić D, Kolašinac S, Rac V, Petrović J, Soković M, Banjac N, Lađarević J, Vidović B, Kostić A, Pavlović V, Pešić M. Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization. in Food Chemistry. 2023;402:134299.
doi:10.1016/j.foodchem.2022.134299 .

Popović Minić, Dušanka, Milinčić, Danijel, Kolašinac, Stefan, Rac, Vladislav, Petrović, Jovana, Soković, Marina, Banjac, Nebojša, Lađarević, Jelena, Vidović, Bojana, Kostić, Aleksandar, Pavlović, Vladimir, Pešić, Mirjana, "Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization" in Food Chemistry, 402 (2023):134299,
https://doi.org/10.1016/j.foodchem.2022.134299 . .

TY  - CONF
AU  - Ćirić, Ana
AU  - Ivanov, Marija
AU  - Petrović, Jovana
AU  - Stojković, Dejan
AU  - Gašić, Uroš
AU  - Milinčić, Danijel
AU  - Pešić, Mirjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6463
AB  - Introduction: The food industry increasingly utilizes plant extracts over synthetic antioxidants for preventing lipid oxidation, fighting foodborne pathogens, and prolonging shelf life of various products. The bioactive potential varies among plant species. Traditionally, extracts from rosehip fruits (Rosa canina L.) have preventive and curative effects on diabetes, memory disorders and cancer. In addition, these extracts are often used as antimicrobial, antinociceptive and anti-inflammatory agents.
Objective: The aim of this work was to investigate phytochemical composition and biological potential of aqueous extracts from the fruits of R. canina. 
Methodology: The lyophilized aqueous extract was analyzed using Agilent 1290 Infinity ultra-high performance liquid chromatography (UHPLC) system in conjunction with quadrupole time-of-flight mass spectrometry. The extracts were analyzed for total polyphenol and flavonoid content (TPC and TFC, respectively) using spectrophotometric methods. The biological activities of aqueous extracts were evaluated under in vitro conditions. The Ferric reducing antioxidant power (FRAP) and DPPH radical scavenging assay were used to investigate antioxidant potential. Antimicrobial activity was determined using microdilution assay and crystal violet and MTT tests were used for evaluation of antibiofilm activity.
Results and conclusions: R. canina fruit's aqueous extract had 32 phenolic compounds, which were classified into eight classes. Qualitative phytochemical analyses showed that this extract contained flavan-3-ols and procyanidins, dominated by epicatechin (624.354 mg/100g) and a proanthocyanidin dimer hexoside type B (457.016 mg/100g). Furthermore, extract was high in total phenolic content (412.25 TE mg/g) but low in total flavonoid compounds (0.73 QE mg/g). The highest antioxidant capacity was measured by FRAP assay (103.29 AAE mg/g), whereas notable antimicrobial properties were determined as well, with a standout 67.68% antibiofilm activity against Candida spp. at 0.5 MIC. These findings highlight potential of R. canina as a dietary source of antioxidants and antimicrobial agents.
PB  - Belgrade:  European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia
C3  - Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia
T1  - Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications
SP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6463
ER  - 

@conference{
author = "Ćirić, Ana and Ivanov, Marija and Petrović, Jovana and Stojković, Dejan and Gašić, Uroš and Milinčić, Danijel and Pešić, Mirjana",
year = "2023",
abstract = "Introduction: The food industry increasingly utilizes plant extracts over synthetic antioxidants for preventing lipid oxidation, fighting foodborne pathogens, and prolonging shelf life of various products. The bioactive potential varies among plant species. Traditionally, extracts from rosehip fruits (Rosa canina L.) have preventive and curative effects on diabetes, memory disorders and cancer. In addition, these extracts are often used as antimicrobial, antinociceptive and anti-inflammatory agents.
Objective: The aim of this work was to investigate phytochemical composition and biological potential of aqueous extracts from the fruits of R. canina. 
Methodology: The lyophilized aqueous extract was analyzed using Agilent 1290 Infinity ultra-high performance liquid chromatography (UHPLC) system in conjunction with quadrupole time-of-flight mass spectrometry. The extracts were analyzed for total polyphenol and flavonoid content (TPC and TFC, respectively) using spectrophotometric methods. The biological activities of aqueous extracts were evaluated under in vitro conditions. The Ferric reducing antioxidant power (FRAP) and DPPH radical scavenging assay were used to investigate antioxidant potential. Antimicrobial activity was determined using microdilution assay and crystal violet and MTT tests were used for evaluation of antibiofilm activity.
Results and conclusions: R. canina fruit's aqueous extract had 32 phenolic compounds, which were classified into eight classes. Qualitative phytochemical analyses showed that this extract contained flavan-3-ols and procyanidins, dominated by epicatechin (624.354 mg/100g) and a proanthocyanidin dimer hexoside type B (457.016 mg/100g). Furthermore, extract was high in total phenolic content (412.25 TE mg/g) but low in total flavonoid compounds (0.73 QE mg/g). The highest antioxidant capacity was measured by FRAP assay (103.29 AAE mg/g), whereas notable antimicrobial properties were determined as well, with a standout 67.68% antibiofilm activity against Candida spp. at 0.5 MIC. These findings highlight potential of R. canina as a dietary source of antioxidants and antimicrobial agents.",
publisher = "Belgrade:  European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia",
journal = "Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia",
title = "Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications",
pages = "41",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6463"
}

Ćirić, A., Ivanov, M., Petrović, J., Stojković, D., Gašić, U., Milinčić, D.,& Pešić, M.. (2023). Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications. in Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia
Belgrade:  European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia., 41.
https://hdl.handle.net/21.15107/rcub_ibiss_6463

Ćirić A, Ivanov M, Petrović J, Stojković D, Gašić U, Milinčić D, Pešić M. Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications. in Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia. 2023;:41.
https://hdl.handle.net/21.15107/rcub_ibiss_6463 .

Ćirić, Ana, Ivanov, Marija, Petrović, Jovana, Stojković, Dejan, Gašić, Uroš, Milinčić, Danijel, Pešić, Mirjana, "Rosehip (Rosa canina L.): A Phytochemical and Bioactive Ingredient Analysis for Functional Food Applications" in Program and Abstract Book: 3rd Black Sea Association of Food Science and Technology Congress: B-FoST; 2023 Dec 13-14; Belgrade, Serbia (2023):41,
https://hdl.handle.net/21.15107/rcub_ibiss_6463 .

TY  - CONF
AU  - Carević, Tamara
AU  - Petrović, Jovana
AU  - Ivanov, Marija
AU  - Milinčić, Danijel
AU  - Pešić, Mirjana
AU  - Ćirić, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6391
AB  - Kontinuirani rast tržišta piva i alkoholnih pića otvorio je nove mogućnosti za poboljšanje ukusa, arome i korisnosti ovih napitaka. Već hiljadu godina unazad je poznato da dodavanje različitih biljnih sastojaka u piće menja njihov ukus i svojstva. Međutim, moderna tehnologija olakšava postizanje željenih karakteristika i nudi nove mogućnosti za terapeutske efekte i senzorna poboljšanja ovog tradicionalnog pića.
U ovom radu su predstavljene hemijska analiza i bioaktivna svojstva etanolnog i vodenog ekstrakta Prunus spinosa L. (trnjine) kao potencijalnog dodatka pivu, sa ciljem povećanja funkcionalnih karakteristika i senzornih osobina ovog napitka.
Hemijske analize korišćenjem UHPLC-QToF-MS pokazale su prisustvo: derivata hidroksibenzoeve kiseline (heksozid vanilinske kiseline do 141.476 mg/100 g ekstrakta), derivata hidroksicimetne kiseline (izomer kafeoilkvinske kiseline do 306.608 mg/100 g ekstrakta, feruloilkvinske kiseline do 225.884 mg/100 g ekstrakta) i derivata flavona (apigenin 180.094 mg/100g ekstrakta).
Procena bioaktivnih svojstava nesumnjivo je pokazala da oba ekstrakta trnjine imaju umeren do dobar antimikrobni potencijal, kao i sposobnost da inhibiraju formiranje biofilma, pri čemu je etanolni ekstrakt pokazao sveukupni bolji potencijal u inhibiciji rasta mikroorganizama. Najbolji antimikrobni potencijal je pokazan kod vodenog ekstrakta prema Bacillus cereus koja je poznati patogen identifikovan u namirnicama (MIC 0.5 mg/mL, MBC 1.00 mg/mL), dok su druge testirane bakterije bile otpornije na aktivnost testiranih ekstrakata (MIC u opsegu od 1.0- 4.0 mg/mL i MBC u opsegu od 2.00-8.00 mg/mL). Rezultati antifungalne aktivnosti su pokazali da ekstrakti prilično ujednačeno deluju inhibitorno na rast testiranih mikrogljiva, uz MIK vrednosti 1.00 mg/mL i MFK 2.00 mg/mL. Detaljnija analiza antimikrobne aktivnosti pokazala je da testirani ekstrakti trnjine značajno inhibiraju formiranje biofilma Candida albicans ATCC 10231; oba testirana uzorka su inhibirala formiranje biofilma na subinhibitornim koncentracijama približno 67.00 %. Uočena aktivnost se može pripisati prisustvu različitih fenolnih jedinjenja.
Imajući u vidu poznat gorak ukus P. spinosa kao i pokazana bioaktivna svojstva njegovih ekstrakata, verujemo da bi ova biljka bila odličan funkcionalni sastojak koji će se dobro uklopiti u jedinstvena senzorna svojstva piva i sličnih napitaka. Predloženo pivo bi čak moglo da pruži dodatne zdravstvene efekte potrošačima, balansirajući između zadovoljstva i njihove želje za zdravim sastojcima.
AB  - The continuous growth of beer and beverage market has opened new opportunities to improve their flavor, aroma, and benefits. It has been known for thousands of years that adding various herbs to beer changes the taste and properties of this beverage. However, modern technology makes it easier to achieve the desired properties and provides useful qualities for therapeutic effects and sensory improvement of traditional beer. This paper presents chemical analysis and bioactive properties of ethanolic and aqueous extract of Prunus spinosa L. (blackthorn) as a potential ingredient for beer to improve functional and sensory properties of this beverage.
Chemical analyses by UHPLC-QToF-MS revealed presence of: hidroxybenzoic acid derivatives (vanillic acid hexoside up to 141.476 mg/100g extract), hidroxycinnamic acid derivatives (caffeoylquinic acid isomer up to 306.608 mg/100g extract and feruloylquinic acid up to 225.884 mg/100g extract) and flavone derivatives (apigenin 180.094 mg/100g extract).
The evaluation of bioactive properties undoubtedly showed that both blackthorn extracts have moderate to rather good antimicrobial and antibiofilm potential, with ethanolic extract being slightly better in terms of inhibiting microbial growth. The best antimicrobial potential was observed with aqueous extract towards foodborne pathogenic bacteria Bacillus cereus (MIC 0.5 mg/mL, MBC 1.00 mg/mL), whereas other tested bacteria were more resilient towards the activity of the tested extracts (MIC in range of 1.0-4.0 mg/mL and MBC in range of 2.00-8.00 mg/mL). In terms of antifungal potential, the extracts showed rather uniform inhibitory effects towards tested microfungi with MIC value 1.00 mg/mL and MFC 2.00 mg/mL. A more in depth analysis of antimicrobial activity showed that the tested blackthorn extracts have promising antibiofilm activity towards Candida albicans ATCC 10231 as well, with both samples inhibiting formation of biofilm at subinhibitory levels approximately 67.00 %. Observed activity may be attributed to the presence of various phenolic compounds. 
Considering the well-known bitter taste of P. spinosa and demonstrated bioactive properties of its extracts, we believe it would be a great functional ingredient to add to the unique sensory properties of beer and similar beverages. This beer may even be able to provide consumers with additional health benefits, balancing enjoyment with the desire for healthy ingredients.
PB  - Novi Sad: Institute of Field and Vegetable Crops
C3  - Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia
T1  - Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima
T1  - Valorization of Prunus spinosa L. extracts as functional ingredients in alcoholic beverages
SP  - 24
EP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6391
ER  - 

@conference{
author = "Carević, Tamara and Petrović, Jovana and Ivanov, Marija and Milinčić, Danijel and Pešić, Mirjana and Ćirić, Ana",
year = "2023",
abstract = "Kontinuirani rast tržišta piva i alkoholnih pića otvorio je nove mogućnosti za poboljšanje ukusa, arome i korisnosti ovih napitaka. Već hiljadu godina unazad je poznato da dodavanje različitih biljnih sastojaka u piće menja njihov ukus i svojstva. Međutim, moderna tehnologija olakšava postizanje željenih karakteristika i nudi nove mogućnosti za terapeutske efekte i senzorna poboljšanja ovog tradicionalnog pića.
U ovom radu su predstavljene hemijska analiza i bioaktivna svojstva etanolnog i vodenog ekstrakta Prunus spinosa L. (trnjine) kao potencijalnog dodatka pivu, sa ciljem povećanja funkcionalnih karakteristika i senzornih osobina ovog napitka.
Hemijske analize korišćenjem UHPLC-QToF-MS pokazale su prisustvo: derivata hidroksibenzoeve kiseline (heksozid vanilinske kiseline do 141.476 mg/100 g ekstrakta), derivata hidroksicimetne kiseline (izomer kafeoilkvinske kiseline do 306.608 mg/100 g ekstrakta, feruloilkvinske kiseline do 225.884 mg/100 g ekstrakta) i derivata flavona (apigenin 180.094 mg/100g ekstrakta).
Procena bioaktivnih svojstava nesumnjivo je pokazala da oba ekstrakta trnjine imaju umeren do dobar antimikrobni potencijal, kao i sposobnost da inhibiraju formiranje biofilma, pri čemu je etanolni ekstrakt pokazao sveukupni bolji potencijal u inhibiciji rasta mikroorganizama. Najbolji antimikrobni potencijal je pokazan kod vodenog ekstrakta prema Bacillus cereus koja je poznati patogen identifikovan u namirnicama (MIC 0.5 mg/mL, MBC 1.00 mg/mL), dok su druge testirane bakterije bile otpornije na aktivnost testiranih ekstrakata (MIC u opsegu od 1.0- 4.0 mg/mL i MBC u opsegu od 2.00-8.00 mg/mL). Rezultati antifungalne aktivnosti su pokazali da ekstrakti prilično ujednačeno deluju inhibitorno na rast testiranih mikrogljiva, uz MIK vrednosti 1.00 mg/mL i MFK 2.00 mg/mL. Detaljnija analiza antimikrobne aktivnosti pokazala je da testirani ekstrakti trnjine značajno inhibiraju formiranje biofilma Candida albicans ATCC 10231; oba testirana uzorka su inhibirala formiranje biofilma na subinhibitornim koncentracijama približno 67.00 %. Uočena aktivnost se može pripisati prisustvu različitih fenolnih jedinjenja.
Imajući u vidu poznat gorak ukus P. spinosa kao i pokazana bioaktivna svojstva njegovih ekstrakata, verujemo da bi ova biljka bila odličan funkcionalni sastojak koji će se dobro uklopiti u jedinstvena senzorna svojstva piva i sličnih napitaka. Predloženo pivo bi čak moglo da pruži dodatne zdravstvene efekte potrošačima, balansirajući između zadovoljstva i njihove želje za zdravim sastojcima., The continuous growth of beer and beverage market has opened new opportunities to improve their flavor, aroma, and benefits. It has been known for thousands of years that adding various herbs to beer changes the taste and properties of this beverage. However, modern technology makes it easier to achieve the desired properties and provides useful qualities for therapeutic effects and sensory improvement of traditional beer. This paper presents chemical analysis and bioactive properties of ethanolic and aqueous extract of Prunus spinosa L. (blackthorn) as a potential ingredient for beer to improve functional and sensory properties of this beverage.
Chemical analyses by UHPLC-QToF-MS revealed presence of: hidroxybenzoic acid derivatives (vanillic acid hexoside up to 141.476 mg/100g extract), hidroxycinnamic acid derivatives (caffeoylquinic acid isomer up to 306.608 mg/100g extract and feruloylquinic acid up to 225.884 mg/100g extract) and flavone derivatives (apigenin 180.094 mg/100g extract).
The evaluation of bioactive properties undoubtedly showed that both blackthorn extracts have moderate to rather good antimicrobial and antibiofilm potential, with ethanolic extract being slightly better in terms of inhibiting microbial growth. The best antimicrobial potential was observed with aqueous extract towards foodborne pathogenic bacteria Bacillus cereus (MIC 0.5 mg/mL, MBC 1.00 mg/mL), whereas other tested bacteria were more resilient towards the activity of the tested extracts (MIC in range of 1.0-4.0 mg/mL and MBC in range of 2.00-8.00 mg/mL). In terms of antifungal potential, the extracts showed rather uniform inhibitory effects towards tested microfungi with MIC value 1.00 mg/mL and MFC 2.00 mg/mL. A more in depth analysis of antimicrobial activity showed that the tested blackthorn extracts have promising antibiofilm activity towards Candida albicans ATCC 10231 as well, with both samples inhibiting formation of biofilm at subinhibitory levels approximately 67.00 %. Observed activity may be attributed to the presence of various phenolic compounds. 
Considering the well-known bitter taste of P. spinosa and demonstrated bioactive properties of its extracts, we believe it would be a great functional ingredient to add to the unique sensory properties of beer and similar beverages. This beer may even be able to provide consumers with additional health benefits, balancing enjoyment with the desire for healthy ingredients.",
publisher = "Novi Sad: Institute of Field and Vegetable Crops",
journal = "Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia",
title = "Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima, Valorization of Prunus spinosa L. extracts as functional ingredients in alcoholic beverages",
pages = "24-27",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6391"
}

Carević, T., Petrović, J., Ivanov, M., Milinčić, D., Pešić, M.,& Ćirić, A.. (2023). Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima. in Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia
Novi Sad: Institute of Field and Vegetable Crops., 24-27.
https://hdl.handle.net/21.15107/rcub_ibiss_6391

Carević T, Petrović J, Ivanov M, Milinčić D, Pešić M, Ćirić A. Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima. in Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia. 2023;:24-27.
https://hdl.handle.net/21.15107/rcub_ibiss_6391 .

Carević, Tamara, Petrović, Jovana, Ivanov, Marija, Milinčić, Danijel, Pešić, Mirjana, Ćirić, Ana, "Vrednovanje ekstrakata Prunus spinosa L. kao funkcionalnih sastojaka u alkoholnim pićima" in Book of abstracts: 6th Scientific-professional symposium with international participation: Beer, brewing raw materials and equipment; 2023 Oct 25-27M Zrenjanin, Serbia (2023):24-27,
https://hdl.handle.net/21.15107/rcub_ibiss_6391 .

TY  - JOUR
AU  - Fernandes, Angela
AU  - Polyzos, Nikolaos
AU  - Mandim, Filipa
AU  - Pereira, Carla
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Petropoulos, Spyridon
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6399
AB  - The aim of the present study was to evaluate the possible effects of two different bios timulant formulations at different application regimes and combined or not with mineral fertilizers
(e.g., W1–W8, including the control treatment (no formulations added)) on the yield parameters and
fruit quality of watermelon plants. The highest yield was recorded for the W5 treatment due to the
formation of more fruit. The highest content of fat, proteins and ash was recorded for treatment
W1, whereas carbohydrates were the most abundant in the control treatment, resulting also in the
highest energetic value. The main detected sugars in all the tested samples were sucrose and fructose,
which were the highest for the W4 and W5 treatments (sucrose) and W4 treatment (fructose). Malic
and citric acid were the most abundant compounds, especially in the W4 treatment. In terms of
tocopherols, only α-tocopherol was detected, with the highest amounts being recorded for the W4
treatment. Regarding bioactive properties, the lowest IC50 values for OxHLIA were recorded for
the W2, W3 and W8 formulations. Moreover, all the extracts exhibited significant anti-inflammatory
activity comparable to the positive control, while a variable efficacy of the tested extracts against
the studied bacteria and fungi was recorded. In conclusion, our results indicate that simple agro nomic practices such as biostimulant application may improve crop performance and improve the
proximal composition and the overall quality of watermelon fruit within the context of sustainable
crop production.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants
IS  - 7
VL  - 9
DO  - 10.3390/horticulturae9070838
SP  - 838
ER  - 

@article{
author = "Fernandes, Angela and Polyzos, Nikolaos and Mandim, Filipa and Pereira, Carla and Petrović, Jovana and Soković, Marina and Petropoulos, Spyridon",
year = "2023",
abstract = "The aim of the present study was to evaluate the possible effects of two different bios timulant formulations at different application regimes and combined or not with mineral fertilizers
(e.g., W1–W8, including the control treatment (no formulations added)) on the yield parameters and
fruit quality of watermelon plants. The highest yield was recorded for the W5 treatment due to the
formation of more fruit. The highest content of fat, proteins and ash was recorded for treatment
W1, whereas carbohydrates were the most abundant in the control treatment, resulting also in the
highest energetic value. The main detected sugars in all the tested samples were sucrose and fructose,
which were the highest for the W4 and W5 treatments (sucrose) and W4 treatment (fructose). Malic
and citric acid were the most abundant compounds, especially in the W4 treatment. In terms of
tocopherols, only α-tocopherol was detected, with the highest amounts being recorded for the W4
treatment. Regarding bioactive properties, the lowest IC50 values for OxHLIA were recorded for
the W2, W3 and W8 formulations. Moreover, all the extracts exhibited significant anti-inflammatory
activity comparable to the positive control, while a variable efficacy of the tested extracts against
the studied bacteria and fungi was recorded. In conclusion, our results indicate that simple agro nomic practices such as biostimulant application may improve crop performance and improve the
proximal composition and the overall quality of watermelon fruit within the context of sustainable
crop production.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants",
number = "7",
volume = "9",
doi = "10.3390/horticulturae9070838",
pages = "838"
}

Fernandes, A., Polyzos, N., Mandim, F., Pereira, C., Petrović, J., Soković, M.,& Petropoulos, S.. (2023). Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants. in Horticulturae
Basel: MDPI., 9(7), 838.
https://doi.org/10.3390/horticulturae9070838

Fernandes A, Polyzos N, Mandim F, Pereira C, Petrović J, Soković M, Petropoulos S. Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants. in Horticulturae. 2023;9(7):838.
doi:10.3390/horticulturae9070838 .

Fernandes, Angela, Polyzos, Nikolaos, Mandim, Filipa, Pereira, Carla, Petrović, Jovana, Soković, Marina, Petropoulos, Spyridon, "Combined Effect of Biostimulants and Mineral Fertilizers on Crop Performance and Fruit Quality of Watermelon Plants" in Horticulturae, 9, no. 7 (2023):838,
https://doi.org/10.3390/horticulturae9070838 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Petrović, Jovana
AU  - Carević, Tamara
AU  - Soković, Marina
AU  - Liaras, Konstantinos
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5793
AB  - This narrative review paper provides an up-to-date overview of the potential of novel synthetic and semisynthetic compounds as antibacterials that target virulence traits in resistant strains. The review focused on research conducted in the last five years and investigated a range of compounds including azoles, indoles, thiophenes, glycopeptides, pleuromutilin derivatives, lactone derivatives, and chalcones. The emergence and spread of antibiotic-resistant bacterial strains is a growing public health concern, and new approaches are urgently needed to combat this threat. One promising approach is to target virulence factors, which are essential for bacterial survival and pathogenesis, but not for bacterial growth. By targeting virulence factors, it may be possible to reduce the severity of bacterial infections without promoting the development of resistance. We discuss the mechanisms of action of the various compounds investigated and their potential as antibacterials. The review highlights the potential of targeting virulence factors as a promising strategy to combat antibiotic resistance and suggests that further research is needed to identify new compounds and optimize their efficacy. The findings of this review suggest that novel synthetic and semisynthetic compounds that target virulence factors have great potential as antibacterials in the fight against antibiotic resistance.
PB  - Basel: MDPI
T2  - Antibiotics
T1  - Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review
IS  - 6
VL  - 12
DO  - 10.3390/antibiotics12060963
SP  - 963
ER  - 

@article{
author = "Stojković, Dejan and Petrović, Jovana and Carević, Tamara and Soković, Marina and Liaras, Konstantinos",
year = "2023",
abstract = "This narrative review paper provides an up-to-date overview of the potential of novel synthetic and semisynthetic compounds as antibacterials that target virulence traits in resistant strains. The review focused on research conducted in the last five years and investigated a range of compounds including azoles, indoles, thiophenes, glycopeptides, pleuromutilin derivatives, lactone derivatives, and chalcones. The emergence and spread of antibiotic-resistant bacterial strains is a growing public health concern, and new approaches are urgently needed to combat this threat. One promising approach is to target virulence factors, which are essential for bacterial survival and pathogenesis, but not for bacterial growth. By targeting virulence factors, it may be possible to reduce the severity of bacterial infections without promoting the development of resistance. We discuss the mechanisms of action of the various compounds investigated and their potential as antibacterials. The review highlights the potential of targeting virulence factors as a promising strategy to combat antibiotic resistance and suggests that further research is needed to identify new compounds and optimize their efficacy. The findings of this review suggest that novel synthetic and semisynthetic compounds that target virulence factors have great potential as antibacterials in the fight against antibiotic resistance.",
publisher = "Basel: MDPI",
journal = "Antibiotics",
title = "Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review",
number = "6",
volume = "12",
doi = "10.3390/antibiotics12060963",
pages = "963"
}

Stojković, D., Petrović, J., Carević, T., Soković, M.,& Liaras, K.. (2023). Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review. in Antibiotics
Basel: MDPI., 12(6), 963.
https://doi.org/10.3390/antibiotics12060963

Stojković D, Petrović J, Carević T, Soković M, Liaras K. Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review. in Antibiotics. 2023;12(6):963.
doi:10.3390/antibiotics12060963 .

Stojković, Dejan, Petrović, Jovana, Carević, Tamara, Soković, Marina, Liaras, Konstantinos, "Synthetic and Semisynthetic Compounds as Antibacterials Targeting Virulence Traits in Resistant Strains: A Narrative Updated Review" in Antibiotics, 12, no. 6 (2023):963,
https://doi.org/10.3390/antibiotics12060963 . .

TY  - JOUR
AU  - Perović, Tatjana
AU  - Petrović, Jovana
AU  - Gašić, Uroš
AU  - Kostić, Marina
AU  - Ćirić, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5498
AB  - Plant extracts and other plant products have been used as an alternative to synthetic fungicides or an additional way to reduce their use. The choice of plant extracts and their application depends on their functional characteristics, availability, cost-effectiveness and their impact on phytopathogens, and also on the environment. Therefore, the present study aims to assess the potential of Celtis australis methanolic extracts as source of compounds with antifungal activity. Methanolic extracts prepared from leaves and unripe mesocarps of C. australis collected from different localities of Montenegro (Podgorica-PG, Donja Gorica-DG, and Bar-BR), were evaluated for their phenolic compounds composition as well as antifungal and cytotoxic properties. Obtained results revealed that extracts contain various bioactive constituents including phenolic acids, flavonoids and their derivatives. The predominant phenolic acid was ferulic acid, identified in leaves samples from DG (187.97 mg/100g dw), while isoorientin was the most abundant phenolic compound found in all examined samples. Regarding antifungal potential of the tested samples, all but one (prepared from mesocarp BR) possessed higher activity, than Previcur, commercial systemic fungicide intended to control seedling. In vitro studies on HaCaT cell line showed that the extracts had no toxic effect towards the tested cell line. These results lead to the conclusion that methanolic extracts of C. australis can become an alternative to the use of synthetic fungicides in agriculture. Those extracts represent natural biodegradable fungicides and enable a more efficient control of pathogenic fungi.
PB  - John Wiley and Sons Inc
T2  - Food Science and Nutrition
T1  - Natural extracts against agricultural pathogens: A case study of Celtis australis L.
DO  - 10.1002/fsn3.3325
ER  - 

@article{
author = "Perović, Tatjana and Petrović, Jovana and Gašić, Uroš and Kostić, Marina and Ćirić, Ana",
year = "2023",
abstract = "Plant extracts and other plant products have been used as an alternative to synthetic fungicides or an additional way to reduce their use. The choice of plant extracts and their application depends on their functional characteristics, availability, cost-effectiveness and their impact on phytopathogens, and also on the environment. Therefore, the present study aims to assess the potential of Celtis australis methanolic extracts as source of compounds with antifungal activity. Methanolic extracts prepared from leaves and unripe mesocarps of C. australis collected from different localities of Montenegro (Podgorica-PG, Donja Gorica-DG, and Bar-BR), were evaluated for their phenolic compounds composition as well as antifungal and cytotoxic properties. Obtained results revealed that extracts contain various bioactive constituents including phenolic acids, flavonoids and their derivatives. The predominant phenolic acid was ferulic acid, identified in leaves samples from DG (187.97 mg/100g dw), while isoorientin was the most abundant phenolic compound found in all examined samples. Regarding antifungal potential of the tested samples, all but one (prepared from mesocarp BR) possessed higher activity, than Previcur, commercial systemic fungicide intended to control seedling. In vitro studies on HaCaT cell line showed that the extracts had no toxic effect towards the tested cell line. These results lead to the conclusion that methanolic extracts of C. australis can become an alternative to the use of synthetic fungicides in agriculture. Those extracts represent natural biodegradable fungicides and enable a more efficient control of pathogenic fungi.",
publisher = "John Wiley and Sons Inc",
journal = "Food Science and Nutrition",
title = "Natural extracts against agricultural pathogens: A case study of Celtis australis L.",
doi = "10.1002/fsn3.3325"
}

Perović, T., Petrović, J., Gašić, U., Kostić, M.,& Ćirić, A.. (2023). Natural extracts against agricultural pathogens: A case study of Celtis australis L.. in Food Science and Nutrition
John Wiley and Sons Inc..
https://doi.org/10.1002/fsn3.3325

Perović T, Petrović J, Gašić U, Kostić M, Ćirić A. Natural extracts against agricultural pathogens: A case study of Celtis australis L.. in Food Science and Nutrition. 2023;.
doi:10.1002/fsn3.3325 .

Perović, Tatjana, Petrović, Jovana, Gašić, Uroš, Kostić, Marina, Ćirić, Ana, "Natural extracts against agricultural pathogens: A case study of Celtis australis L." in Food Science and Nutrition (2023),
https://doi.org/10.1002/fsn3.3325 . .

TY  - JOUR
AU  - Božunović, Jelena
AU  - Ivanov, Marija
AU  - Petrović, Jovana
AU  - Gašić, Uroš
AU  - Nakarada, Đura
AU  - Milutinović, Milica
AU  - Aničić, Neda
AU  - Giba, Zlatko
AU  - Mišić, Danijela
AU  - Stojković, Dejan
PY  - 2023
UR  - https://www.mdpi.com/1424-8247/16/2/245
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5490
AB  - Spiked centaury (Centaurium spicatum) is a well-known medicinal plant from the Mediterranean region with various bioactivities, but there are no studies addressing the use of different solvent systems to improve its pharmacological potential. Nine extraction procedures were adapted to study the effects of solvent composition on the content of bioactive compounds in C. spicatum extracts and on corresponding bioactivities. Targeted metabolomics was performed to obtain information on the chemical composition of extracts. Ethanol-water-based extraction procedures were the most efficient in isolating polyphenols, while less polar butanol extract contained the highest amount of iridoids. Antioxidant potential analysis revealed stronger activity in extracts with higher polyphenol content. Bacillus cereus and Staphylococus aureus were designated as the most sensitive bacterial strains to the activity of extracts, while among the micromycetes tested, Penicillium funiculosum was the most susceptible strain. Butanol extract showed antivirulence potential on Candida albicans morphological transition from yeast to hyphal form, and selected extracts were effective against biofilm formation in two Candida species. All the extracts tested in this study showed no cytotoxic activity to immortalize human skin keratinocyte cell line (HaCaT), whereas extracts obtained by ethanol-water extraction stand out for their potent wound healing effects. Moreover, the influence of the extraction solvent system on various bioactivities of C. spicatum is reported herein for the first time. Overall, the results presented in this study promote the use of C. spicatum as a source of natural products with potential antioxidant, wound healing, and antimicrobial applications that are potentially safe for human use.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts
IS  - 2
VL  - 16
DO  - 10.3390/ph16020245
SP  - 245
ER  - 

@article{
author = "Božunović, Jelena and Ivanov, Marija and Petrović, Jovana and Gašić, Uroš and Nakarada, Đura and Milutinović, Milica and Aničić, Neda and Giba, Zlatko and Mišić, Danijela and Stojković, Dejan",
year = "2023",
abstract = "Spiked centaury (Centaurium spicatum) is a well-known medicinal plant from the Mediterranean region with various bioactivities, but there are no studies addressing the use of different solvent systems to improve its pharmacological potential. Nine extraction procedures were adapted to study the effects of solvent composition on the content of bioactive compounds in C. spicatum extracts and on corresponding bioactivities. Targeted metabolomics was performed to obtain information on the chemical composition of extracts. Ethanol-water-based extraction procedures were the most efficient in isolating polyphenols, while less polar butanol extract contained the highest amount of iridoids. Antioxidant potential analysis revealed stronger activity in extracts with higher polyphenol content. Bacillus cereus and Staphylococus aureus were designated as the most sensitive bacterial strains to the activity of extracts, while among the micromycetes tested, Penicillium funiculosum was the most susceptible strain. Butanol extract showed antivirulence potential on Candida albicans morphological transition from yeast to hyphal form, and selected extracts were effective against biofilm formation in two Candida species. All the extracts tested in this study showed no cytotoxic activity to immortalize human skin keratinocyte cell line (HaCaT), whereas extracts obtained by ethanol-water extraction stand out for their potent wound healing effects. Moreover, the influence of the extraction solvent system on various bioactivities of C. spicatum is reported herein for the first time. Overall, the results presented in this study promote the use of C. spicatum as a source of natural products with potential antioxidant, wound healing, and antimicrobial applications that are potentially safe for human use.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts",
number = "2",
volume = "16",
doi = "10.3390/ph16020245",
pages = "245"
}

Božunović, J., Ivanov, M., Petrović, J., Gašić, U., Nakarada, Đ., Milutinović, M., Aničić, N., Giba, Z., Mišić, D.,& Stojković, D.. (2023). Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts. in Pharmaceuticals
Basel: MDPI., 16(2), 245.
https://doi.org/10.3390/ph16020245

Božunović J, Ivanov M, Petrović J, Gašić U, Nakarada Đ, Milutinović M, Aničić N, Giba Z, Mišić D, Stojković D. Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts. in Pharmaceuticals. 2023;16(2):245.
doi:10.3390/ph16020245 .

Božunović, Jelena, Ivanov, Marija, Petrović, Jovana, Gašić, Uroš, Nakarada, Đura, Milutinović, Milica, Aničić, Neda, Giba, Zlatko, Mišić, Danijela, Stojković, Dejan, "Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts" in Pharmaceuticals, 16, no. 2 (2023):245,
https://doi.org/10.3390/ph16020245 . .

TY  - CONF
AU  - Stojković, Dejan
AU  - Ivanov, Marija
AU  - Ćirić, Ana
AU  - Petrović, Jovana
AU  - Kostić, Marina
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5158
AB  - Chicory (Cichorium intybus L.) is an important industrial crop cultivated mainly to extract the
dietary fiber inulin. This study aimed to investigate antimicrobial, antibiofilm and wound healing
potentials of the extract obtained by supercritical fluid extraction (SFE). Antibacterial activity
of extract ranged from 0.25 to 4.00 mg/mL regarding minimal inhibitory concentra-tions (MICs),
while minimal bactericidal concentrations (MBCs) were noted within the range of 0.5 to 8.00 mg/
mL. The extract has shown promising antifungal potential with MICs rang-ing 0.125- 0.75 mg/
mL and MFCs 0.25-1.0 mg/mL against Candida species. Incubation with the extract has reduced
the ability of both C. albicans and P. aeruginosa to attach to the sur-face and begin the process
of biofilm formation. Concentration equal to previously deter-mined MIC has almost completely
(>90% inhibition) reduced the biofilm biomass formed by P. aeruginosa. Even twice as lower concentrations
(0.5 MIC) has limited the biofilm forming ability and induce more than 80% inhibition
in P. aeruginosa as bacterial biofilm model or-ganism. Impact on fungal biofilm was less profound
and MIC concentration was able to re-duce the biofilm formation for less than 60%. HaCaT (human
immortalized keratinocytes cell line) cells were treated with IC20 (24 h) concentration of chicory
SFE extract. It is estimated that SFE extract has slight effect on wound closure in keratinocyte
monolayer, when com-pared to untreated control. Since, Chicory SFE fraction possessed excellent
antibacterial po-tential against E. coli and K. pneumoniae and antifungal activity against Candida
species these results are considered very important in the future inventions
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
T1  - Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5158
ER  - 

@conference{
author = "Stojković, Dejan and Ivanov, Marija and Ćirić, Ana and Petrović, Jovana and Kostić, Marina and Glamočlija, Jasmina and Soković, Marina",
year = "2022",
abstract = "Chicory (Cichorium intybus L.) is an important industrial crop cultivated mainly to extract the
dietary fiber inulin. This study aimed to investigate antimicrobial, antibiofilm and wound healing
potentials of the extract obtained by supercritical fluid extraction (SFE). Antibacterial activity
of extract ranged from 0.25 to 4.00 mg/mL regarding minimal inhibitory concentra-tions (MICs),
while minimal bactericidal concentrations (MBCs) were noted within the range of 0.5 to 8.00 mg/
mL. The extract has shown promising antifungal potential with MICs rang-ing 0.125- 0.75 mg/
mL and MFCs 0.25-1.0 mg/mL against Candida species. Incubation with the extract has reduced
the ability of both C. albicans and P. aeruginosa to attach to the sur-face and begin the process
of biofilm formation. Concentration equal to previously deter-mined MIC has almost completely
(>90% inhibition) reduced the biofilm biomass formed by P. aeruginosa. Even twice as lower concentrations
(0.5 MIC) has limited the biofilm forming ability and induce more than 80% inhibition
in P. aeruginosa as bacterial biofilm model or-ganism. Impact on fungal biofilm was less profound
and MIC concentration was able to re-duce the biofilm formation for less than 60%. HaCaT (human
immortalized keratinocytes cell line) cells were treated with IC20 (24 h) concentration of chicory
SFE extract. It is estimated that SFE extract has slight effect on wound closure in keratinocyte
monolayer, when com-pared to untreated control. Since, Chicory SFE fraction possessed excellent
antibacterial po-tential against E. coli and K. pneumoniae and antifungal activity against Candida
species these results are considered very important in the future inventions",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia",
title = "Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials",
pages = "96",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5158"
}

Stojković, D., Ivanov, M., Ćirić, A., Petrović, J., Kostić, M., Glamočlija, J.,& Soković, M.. (2022). Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 96.
https://hdl.handle.net/21.15107/rcub_ibiss_5158

Stojković D, Ivanov M, Ćirić A, Petrović J, Kostić M, Glamočlija J, Soković M. Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials. in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. 2022;:96.
https://hdl.handle.net/21.15107/rcub_ibiss_5158 .

Stojković, Dejan, Ivanov, Marija, Ćirić, Ana, Petrović, Jovana, Kostić, Marina, Glamočlija, Jasmina, Soković, Marina, "Supercritical fluid extraction of Chicory reveals its antimicrobial, antibiofilm and wound healing potentials" in 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia (2022):96,
https://hdl.handle.net/21.15107/rcub_ibiss_5158 .

TY  - CONF
AU  - Ćirić, Ana
AU  - Ivanov, Marija
AU  - Stojković, Dejan
AU  - Glamočlija, Jasmina
AU  - Kostić, Marina
AU  - Petrović, Jovana
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5581
AB  - Cichorium intybus L. (Asteraceae), is an important medicinal plant, rich in sesquiterpene
lactones (STLs), inulin, biologically relevant elements, and vitamins which exert potent
pro-health effects on the human organism. The aim of this study was to screen two
extracts of chicory containing STLs, the optimized extract (Ci_SFEopt) and the
supercritical fluid extract (Ci_SFEpur), for their antimicrobial activities against a range
of pathogenic microorganisms. Both extracts showed antibacterial activity, with
Ci_SFEpur being more potent than Ci_SFEopt. The most sensitive species was
Pseudomonas aeruginosa (minimal inhibitory concentration, MIC 0.06 mg/mL).
Ci_SFEpur and Ci_SFEopt extracts demonstrated good antifungal activity against
Candida species (MIC 0.03-1.00 mg/mL). Thin layer chromatography (TLC)
bioautography was used to screen the extracts and provide basic information for the
isolation of bioactive compounds in the future. Ci_SFEopt was active against all tested
microorganisms (Rf values were 0.18-0.88). The Ci_SFEpur extract efficiently inhibited
(Rf 0.76) Staphylococcus aureus, P. aeruginosa and Candida krusei. The identification
of powerful antimicrobial activity of Ci_SFEpur against P. aeruginosa and Candida
species is an important step towards the development of novel antimicrobial therapeutics
and efficient utilization of this widely distributed plant.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Evaluation of antimicrobial properties of chicory extracts
SP  - 257
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5581
ER  - 

@conference{
author = "Ćirić, Ana and Ivanov, Marija and Stojković, Dejan and Glamočlija, Jasmina and Kostić, Marina and Petrović, Jovana and Soković, Marina",
year = "2022",
abstract = "Cichorium intybus L. (Asteraceae), is an important medicinal plant, rich in sesquiterpene
lactones (STLs), inulin, biologically relevant elements, and vitamins which exert potent
pro-health effects on the human organism. The aim of this study was to screen two
extracts of chicory containing STLs, the optimized extract (Ci_SFEopt) and the
supercritical fluid extract (Ci_SFEpur), for their antimicrobial activities against a range
of pathogenic microorganisms. Both extracts showed antibacterial activity, with
Ci_SFEpur being more potent than Ci_SFEopt. The most sensitive species was
Pseudomonas aeruginosa (minimal inhibitory concentration, MIC 0.06 mg/mL).
Ci_SFEpur and Ci_SFEopt extracts demonstrated good antifungal activity against
Candida species (MIC 0.03-1.00 mg/mL). Thin layer chromatography (TLC)
bioautography was used to screen the extracts and provide basic information for the
isolation of bioactive compounds in the future. Ci_SFEopt was active against all tested
microorganisms (Rf values were 0.18-0.88). The Ci_SFEpur extract efficiently inhibited
(Rf 0.76) Staphylococcus aureus, P. aeruginosa and Candida krusei. The identification
of powerful antimicrobial activity of Ci_SFEpur against P. aeruginosa and Candida
species is an important step towards the development of novel antimicrobial therapeutics
and efficient utilization of this widely distributed plant.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Evaluation of antimicrobial properties of chicory extracts",
pages = "257",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5581"
}

Ćirić, A., Ivanov, M., Stojković, D., Glamočlija, J., Kostić, M., Petrović, J.,& Soković, M.. (2022). Evaluation of antimicrobial properties of chicory extracts. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 257.
https://hdl.handle.net/21.15107/rcub_ibiss_5581

Ćirić A, Ivanov M, Stojković D, Glamočlija J, Kostić M, Petrović J, Soković M. Evaluation of antimicrobial properties of chicory extracts. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:257.
https://hdl.handle.net/21.15107/rcub_ibiss_5581 .

Ćirić, Ana, Ivanov, Marija, Stojković, Dejan, Glamočlija, Jasmina, Kostić, Marina, Petrović, Jovana, Soković, Marina, "Evaluation of antimicrobial properties of chicory extracts" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):257,
https://hdl.handle.net/21.15107/rcub_ibiss_5581 .

TY  - CONF
AU  - Kostić, Marina
AU  - Petrović, Jovana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5282
AB  - Veliki broj makrogljiva predstavlja nedovoljno iskorišćen izvor jedinjenja koja mogu imati potencijalnu primenu u medicini, farmaciji i različitim granama privrede. Pored toga što imaju povoljan odnos makronutrijenata, važan su izvor visoko cenjenih polisaharida, lektina, terpenoida, peptida, itd. Ova jedinjenja su odgovorna za mnogobrojne biološke aktivnosti uključujući antineurodegenerativnu, imunomodulatornu, antimikrobnu, antihiperglikemijsku aktivnost. Danas su u upotrebi različiti dijetetski proizvodi na bazi makrogljiva koji se koriste za prevenciju i ublažavanje simptoma različitih oboljenja. Iako ovi proizvodi ne mogu u potpunosti zameniti komercijalne lekove, u kombinaciji sa njihovom primenom mogu da dovedu do poboljšanja opšteg stanja. Zbog navedenog, autohtone samonikle, ali i komercijalno gajene vrste makrogljiva predstavljaju važan i još uvek nedovoljno istražen izvor bioloških aktivnih supstanci. Štaviše, one imaju ogroman potencijal kao profilaktički agensi kod bolesti čiji je osnovni uzrok povezan sa oksidativnim stresom, bakterijskim i gljivičnim infekcijama kao i pojavom/razvojem različitih tipova tumora. Imajući u vidu da su hranljiva i lekovita svojstva jestivih i lekovitih gljiva hvaljena kako u etnomedicini tako i u naučnim/kliničkim istraživanjima, ova svojstva će biti predstavljena kako bi se procenili novi trendovi i moguća primena jestivih i lekovitih gljiva sa teritorije Srbije u medicini, farmaceutskoj i prehrambenoj industriji.
AB  - Велики број макрогљива представља недовољно искоришћен извор једињења која могу имати потенцијалну примену у медицини, фармацији и различитим гранама привреде. Поред тога што имају повољан однос макронутријената, важан су извор високо цењених полисахарида, лектина, терпеноида, пептида, итд. Ова једињења су одговорна за многобројне биолошке активности укључујући антинеуродегенеративну, имуномодулаторну, антимикробну, антихипергликемијску активност. Данас су у употреби различити дијeтетски производи на бази макрогљива који се користе за превенцију и ублажавање симптома различитих обољења. Иако ови производи не могу у потпуности заменити комерцијалне лекове, у комбинацији са њиховом применом могу да доведу до побољшања општег стања. Због наведеног, аутохтоне самоникле, али и комерцијално гајене врсте макрогљива представљају важан и још увек недовољно истражен извор биолошких активних супстанци. Штавише, оне имају огроман потенцијал као профилактички агенси код болести чији је основни узрок повезан са оксидативним стресом, бактеријским и гљивичним инфекцијама као и појавом/развојем различитих типова тумора. Имајући у виду да су хранљива и лековита својства јестивих и лековитих гљива хваљена како у етномедицини тако и у научним/клиничким истраживањима, ова својства ће бити представљена како би се проценили нови трендови и могућа примена јестивих и лековитих гљива са територије Србије у медицини, фармацеутској и прехрамбеној индустрији.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Biološki potencijal gljiva sa teritorije Srbije
T1  - Биолошки потенцијал гљива са територије Србије
SP  - 227
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5282
ER  - 

@conference{
author = "Kostić, Marina and Petrović, Jovana and Glamočlija, Jasmina and Soković, Marina",
year = "2022",
abstract = "Veliki broj makrogljiva predstavlja nedovoljno iskorišćen izvor jedinjenja koja mogu imati potencijalnu primenu u medicini, farmaciji i različitim granama privrede. Pored toga što imaju povoljan odnos makronutrijenata, važan su izvor visoko cenjenih polisaharida, lektina, terpenoida, peptida, itd. Ova jedinjenja su odgovorna za mnogobrojne biološke aktivnosti uključujući antineurodegenerativnu, imunomodulatornu, antimikrobnu, antihiperglikemijsku aktivnost. Danas su u upotrebi različiti dijetetski proizvodi na bazi makrogljiva koji se koriste za prevenciju i ublažavanje simptoma različitih oboljenja. Iako ovi proizvodi ne mogu u potpunosti zameniti komercijalne lekove, u kombinaciji sa njihovom primenom mogu da dovedu do poboljšanja opšteg stanja. Zbog navedenog, autohtone samonikle, ali i komercijalno gajene vrste makrogljiva predstavljaju važan i još uvek nedovoljno istražen izvor bioloških aktivnih supstanci. Štaviše, one imaju ogroman potencijal kao profilaktički agensi kod bolesti čiji je osnovni uzrok povezan sa oksidativnim stresom, bakterijskim i gljivičnim infekcijama kao i pojavom/razvojem različitih tipova tumora. Imajući u vidu da su hranljiva i lekovita svojstva jestivih i lekovitih gljiva hvaljena kako u etnomedicini tako i u naučnim/kliničkim istraživanjima, ova svojstva će biti predstavljena kako bi se procenili novi trendovi i moguća primena jestivih i lekovitih gljiva sa teritorije Srbije u medicini, farmaceutskoj i prehrambenoj industriji., Велики број макрогљива представља недовољно искоришћен извор једињења која могу имати потенцијалну примену у медицини, фармацији и различитим гранама привреде. Поред тога што имају повољан однос макронутријената, важан су извор високо цењених полисахарида, лектина, терпеноида, пептида, итд. Ова једињења су одговорна за многобројне биолошке активности укључујући антинеуродегенеративну, имуномодулаторну, антимикробну, антихипергликемијску активност. Данас су у употреби различити дијeтетски производи на бази макрогљива који се користе за превенцију и ублажавање симптома различитих обољења. Иако ови производи не могу у потпуности заменити комерцијалне лекове, у комбинацији са њиховом применом могу да доведу до побољшања општег стања. Због наведеног, аутохтоне самоникле, али и комерцијално гајене врсте макрогљива представљају важан и још увек недовољно истражен извор биолошких активних супстанци. Штавише, оне имају огроман потенцијал као профилактички агенси код болести чији је основни узрок повезан са оксидативним стресом, бактеријским и гљивичним инфекцијама као и појавом/развојем различитих типова тумора. Имајући у виду да су хранљива и лековита својства јестивих и лековитих гљива хваљена како у етномедицини тако и у научним/клиничким истраживањима, ова својства ће бити представљена како би се проценили нови трендови и могућа примена јестивих и лековитих гљива са територије Србије у медицини, фармацеутској и прехрамбеној индустрији.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Biološki potencijal gljiva sa teritorije Srbije, Биолошки потенцијал гљива са територије Србије",
pages = "227",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5282"
}

Kostić, M., Petrović, J., Glamočlija, J.,& Soković, M.. (2022). Biološki potencijal gljiva sa teritorije Srbije. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 227.
https://hdl.handle.net/21.15107/rcub_ibiss_5282

Kostić M, Petrović J, Glamočlija J, Soković M. Biološki potencijal gljiva sa teritorije Srbije. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:227.
https://hdl.handle.net/21.15107/rcub_ibiss_5282 .

Kostić, Marina, Petrović, Jovana, Glamočlija, Jasmina, Soković, Marina, "Biološki potencijal gljiva sa teritorije Srbije" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):227,
https://hdl.handle.net/21.15107/rcub_ibiss_5282 .

TY  - JOUR
AU  - Vieira, Dalila M.
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Barros, Lillian
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Barreiro, Maria Filomena
AU  - Ferreira, Isabel C.F.R.
AU  - Castro, Maria Cidalia R.
AU  - Rodrigues, Pedro V.
AU  - Machado, Ana Vera
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1002/fft2.141
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5435
AB  - Natural extracts have been used in several traditional medicine applications and culinary purposes. Their biological properties (antioxidant and antimicrobial) are due to the presence of several active aromatic compounds. Herein, different natural extracts were evaluated, namely structural and thermal characterization and biological activity, in its natural form and incorporated into a polymeric matrix, to assess their effective potential as additives for active food packaging. While rosemary presented the highest thermal stability with a degradation starting at 327°C, lemon balm extract was the less stable (180°C). Regarding the thiobarbituric acid assay, all extracts presented antioxidant activity, in oxidative hemolysis inhibition; anise, cinnamon, and clove extract did not present any action. Overall, the results demonstrated that leaves (rosemary and green tea) and the rhizome (curcumin) are the plant parts with the best performance. Therefore, extracts from aromatic plants are promising natural additives that can be incorporated into polymeric matrices to produce active food packaging film, increasing products shelf-life.
PB  - Hoboken: Wiley
T2  - Food Frontiers
T1  - Evaluation of plant extracts as an efficient source of additives for active food packaging
IS  - 3
VL  - 3
DO  - 10.1002/fft2.141
SP  - 480
EP  - 488
ER  - 

@article{
author = "Vieira, Dalila M. and Pereira, Carla and Calhelha, Ricardo C. and Barros, Lillian and Petrović, Jovana and Soković, Marina and Barreiro, Maria Filomena and Ferreira, Isabel C.F.R. and Castro, Maria Cidalia R. and Rodrigues, Pedro V. and Machado, Ana Vera",
year = "2022",
abstract = "Natural extracts have been used in several traditional medicine applications and culinary purposes. Their biological properties (antioxidant and antimicrobial) are due to the presence of several active aromatic compounds. Herein, different natural extracts were evaluated, namely structural and thermal characterization and biological activity, in its natural form and incorporated into a polymeric matrix, to assess their effective potential as additives for active food packaging. While rosemary presented the highest thermal stability with a degradation starting at 327°C, lemon balm extract was the less stable (180°C). Regarding the thiobarbituric acid assay, all extracts presented antioxidant activity, in oxidative hemolysis inhibition; anise, cinnamon, and clove extract did not present any action. Overall, the results demonstrated that leaves (rosemary and green tea) and the rhizome (curcumin) are the plant parts with the best performance. Therefore, extracts from aromatic plants are promising natural additives that can be incorporated into polymeric matrices to produce active food packaging film, increasing products shelf-life.",
publisher = "Hoboken: Wiley",
journal = "Food Frontiers",
title = "Evaluation of plant extracts as an efficient source of additives for active food packaging",
number = "3",
volume = "3",
doi = "10.1002/fft2.141",
pages = "480-488"
}

Vieira, D. M., Pereira, C., Calhelha, R. C., Barros, L., Petrović, J., Soković, M., Barreiro, M. F., Ferreira, I. C.F.R., Castro, M. C. R., Rodrigues, P. V.,& Machado, A. V.. (2022). Evaluation of plant extracts as an efficient source of additives for active food packaging. in Food Frontiers
Hoboken: Wiley., 3(3), 480-488.
https://doi.org/10.1002/fft2.141

Vieira DM, Pereira C, Calhelha RC, Barros L, Petrović J, Soković M, Barreiro MF, Ferreira IC, Castro MCR, Rodrigues PV, Machado AV. Evaluation of plant extracts as an efficient source of additives for active food packaging. in Food Frontiers. 2022;3(3):480-488.
doi:10.1002/fft2.141 .

Vieira, Dalila M., Pereira, Carla, Calhelha, Ricardo C., Barros, Lillian, Petrović, Jovana, Soković, Marina, Barreiro, Maria Filomena, Ferreira, Isabel C.F.R., Castro, Maria Cidalia R., Rodrigues, Pedro V., Machado, Ana Vera, "Evaluation of plant extracts as an efficient source of additives for active food packaging" in Food Frontiers, 3, no. 3 (2022):480-488,
https://doi.org/10.1002/fft2.141 . .

TY  - CONF
AU  - Petrović, Jovana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5755
AB  - Ever since the origin of the first man, food has been used not only to satisfy hunger, but to provide nutrition. However, it seems as thoughtful eating, with enjoying in food for nourishment is currently in the backgorund, and only hedonic experience of food in mind. Medicine and science revealed that the malnutrition on one side, and overeating on the other, are responsible for various diet related issues, such as: vitamins deficiency, scurvy, pellagra (in case poor diet) or coronary heart disease, high blood pressure, atherosclerosis, type 2 diabetes etc. (in case of excessive food intake). Nevertheless, the awareness on how eating habits influence health status has been set out as a trend only recently, opening up new avenues for the food industry development. This has been largely influenced by the increasing demand for healthy, nutritious and tasteful food of sustainable origin, which aside from fulfilling basic nutritional needs, also have health beneficial properties, thus fitting into the concept of functional food. Mushrooms emerged as rather versatile food which blend equally well with both sweet and savory ingredients, with nutritional richness, low caloric value, taste, and nutraceutical properties; their intake satisfies need for both nutrient and non-nutrient compounds that benefit human health in several aspects making them excellent candidates for functional food. Thus, regular consumption of mushrooms will provide recommended amount of fiber and other carbohydrates, proteins and contribute to daily intake of vitamins (B1, B2, B12, C, D, E), as well as polyunsatturated fatty acids. Along with this, it will supply with minerals (iron, copper, manganese, zinc) as well as terpenoids, phenolic and flavonoid compounds, which do not contribute to nutritive features of mushrooms, but play an important role in proper functioning of different metabolic pathways and/or have bioactive properties. Recent fundamental and clinical research highlighted potential of mushrooms as source of compounds with wide ranging bioactivities, including antimicrobial, antitumor, antiinflammatory, hepatoprotective, neuroprotective, cardioprotective, imunostimulatory etc. 
Palatability, versatility and bioactivity of wild growing and cultivated mushrooms with high demand of consumers for novel, tasteful and nutritive products, put them in the spotlight of the contemporary food industry, which led to the development of innovative designing mushroom-based food and beverages with functional food properties. Furthermore, extractability of bioactive/nutritive compounds from mushrooms enables their availability in form of pills/capsules which allows consumption in populations with limited accessibility to these highly valued ingredients. After all, since cultivation of mushrooms increases the amount of this underutilized food products and permits circular economy, it is expected that mushrooms and/or mushroom based food ingredients will soon be accessible to everyone.
PB  - Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia
C3  - Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
T1  - Mushrooms as source of functional food ingredients
SP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5755
ER  - 

@conference{
author = "Petrović, Jovana",
year = "2022",
abstract = "Ever since the origin of the first man, food has been used not only to satisfy hunger, but to provide nutrition. However, it seems as thoughtful eating, with enjoying in food for nourishment is currently in the backgorund, and only hedonic experience of food in mind. Medicine and science revealed that the malnutrition on one side, and overeating on the other, are responsible for various diet related issues, such as: vitamins deficiency, scurvy, pellagra (in case poor diet) or coronary heart disease, high blood pressure, atherosclerosis, type 2 diabetes etc. (in case of excessive food intake). Nevertheless, the awareness on how eating habits influence health status has been set out as a trend only recently, opening up new avenues for the food industry development. This has been largely influenced by the increasing demand for healthy, nutritious and tasteful food of sustainable origin, which aside from fulfilling basic nutritional needs, also have health beneficial properties, thus fitting into the concept of functional food. Mushrooms emerged as rather versatile food which blend equally well with both sweet and savory ingredients, with nutritional richness, low caloric value, taste, and nutraceutical properties; their intake satisfies need for both nutrient and non-nutrient compounds that benefit human health in several aspects making them excellent candidates for functional food. Thus, regular consumption of mushrooms will provide recommended amount of fiber and other carbohydrates, proteins and contribute to daily intake of vitamins (B1, B2, B12, C, D, E), as well as polyunsatturated fatty acids. Along with this, it will supply with minerals (iron, copper, manganese, zinc) as well as terpenoids, phenolic and flavonoid compounds, which do not contribute to nutritive features of mushrooms, but play an important role in proper functioning of different metabolic pathways and/or have bioactive properties. Recent fundamental and clinical research highlighted potential of mushrooms as source of compounds with wide ranging bioactivities, including antimicrobial, antitumor, antiinflammatory, hepatoprotective, neuroprotective, cardioprotective, imunostimulatory etc. 
Palatability, versatility and bioactivity of wild growing and cultivated mushrooms with high demand of consumers for novel, tasteful and nutritive products, put them in the spotlight of the contemporary food industry, which led to the development of innovative designing mushroom-based food and beverages with functional food properties. Furthermore, extractability of bioactive/nutritive compounds from mushrooms enables their availability in form of pills/capsules which allows consumption in populations with limited accessibility to these highly valued ingredients. After all, since cultivation of mushrooms increases the amount of this underutilized food products and permits circular economy, it is expected that mushrooms and/or mushroom based food ingredients will soon be accessible to everyone.",
publisher = "Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia",
journal = "Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia",
title = "Mushrooms as source of functional food ingredients",
pages = "20",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5755"
}

Petrović, J.. (2022). Mushrooms as source of functional food ingredients. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia
Belgrade: European Hygienic Engineering and Design Group (EHEDG) - Regional Section Serbia., 20.
https://hdl.handle.net/21.15107/rcub_ibiss_5755

Petrović J. Mushrooms as source of functional food ingredients. in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia. 2022;:20.
https://hdl.handle.net/21.15107/rcub_ibiss_5755 .

Petrović, Jovana, "Mushrooms as source of functional food ingredients" in Electronic book of abstracts: 11th International Medicinal mushroom conference IMMC11; 2022 Sep 27-30; Belgrade, Serbia (2022):20,
https://hdl.handle.net/21.15107/rcub_ibiss_5755 .

TY  - JOUR
AU  - Kostić, Aleksandar Ž.
AU  - Mačukanović-Jocić, Marina P
AU  - Milinčić, Danijel D.
AU  - Petrović, Jovana
AU  - Gašić, Uroš
AU  - Gligorijević, Nevenka N.
AU  - Jarić, Snežana
AU  - Soković, Marina
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1002/cbdv.202200069
UR  - http://www.ncbi.nlm.nih.gov/pubmed/35213785
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4941
AB  - The current study aimed to phytochemically characterize (including a detailed phenolic profile) two endemic Balkan's species (Hieracium waldsteinii and Onosma stellulata) and determine their possible application as a source of natural antioxidant and antimicrobial agents. The main phenolic compound in both species (in all examined parts) was chlorogenic acid. Eriodictyol, genistein and naringenin were quantified only in H. waldsteinii while isorhamnetin-3-O-rutinoside and sinapic acid were characteristic for O. stellulata. The highest antioxidant activity (98 mg AAE/g dry weight for TAC assay) was ascribed to the flower extract of H. waldsteinii while the lowest results (∼4.3 mg AAE/g dry weight for FRP assay) were exhibited by the extracts obtained from the plant's stem. Antimicrobial assays showed moderate antibacterial, i. e., moderate/strong activity against several tested fungi (in particular Trichoderma viride). Correlation analysis revealed strong positive connection between phenolic compounds and reducing power of extracts as well as between total phenolic and flavonoid content and the obtained minimal inhibitory concentration recorded in antibacterial assays.
T2  - Chemistry & Biodiversity
T1  - Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents.
IS  - 4
VL  - 19
DO  - 10.1002/cbdv.202200069
SP  - e202200069
ER  - 

@article{
author = "Kostić, Aleksandar Ž. and Mačukanović-Jocić, Marina P and Milinčić, Danijel D. and Petrović, Jovana and Gašić, Uroš and Gligorijević, Nevenka N. and Jarić, Snežana and Soković, Marina and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2022",
abstract = "The current study aimed to phytochemically characterize (including a detailed phenolic profile) two endemic Balkan's species (Hieracium waldsteinii and Onosma stellulata) and determine their possible application as a source of natural antioxidant and antimicrobial agents. The main phenolic compound in both species (in all examined parts) was chlorogenic acid. Eriodictyol, genistein and naringenin were quantified only in H. waldsteinii while isorhamnetin-3-O-rutinoside and sinapic acid were characteristic for O. stellulata. The highest antioxidant activity (98 mg AAE/g dry weight for TAC assay) was ascribed to the flower extract of H. waldsteinii while the lowest results (∼4.3 mg AAE/g dry weight for FRP assay) were exhibited by the extracts obtained from the plant's stem. Antimicrobial assays showed moderate antibacterial, i. e., moderate/strong activity against several tested fungi (in particular Trichoderma viride). Correlation analysis revealed strong positive connection between phenolic compounds and reducing power of extracts as well as between total phenolic and flavonoid content and the obtained minimal inhibitory concentration recorded in antibacterial assays.",
journal = "Chemistry & Biodiversity",
title = "Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents.",
number = "4",
volume = "19",
doi = "10.1002/cbdv.202200069",
pages = "e202200069"
}

Kostić, A. Ž., Mačukanović-Jocić, M. P., Milinčić, D. D., Petrović, J., Gašić, U., Gligorijević, N. N., Jarić, S., Soković, M., Tešić, Ž. Lj.,& Pešić, M. B.. (2022). Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents.. in Chemistry & Biodiversity, 19(4), e202200069.
https://doi.org/10.1002/cbdv.202200069

Kostić AŽ, Mačukanović-Jocić MP, Milinčić DD, Petrović J, Gašić U, Gligorijević NN, Jarić S, Soković M, Tešić ŽL, Pešić MB. Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents.. in Chemistry & Biodiversity. 2022;19(4):e202200069.
doi:10.1002/cbdv.202200069 .

Kostić, Aleksandar Ž., Mačukanović-Jocić, Marina P, Milinčić, Danijel D., Petrović, Jovana, Gašić, Uroš, Gligorijević, Nevenka N., Jarić, Snežana, Soković, Marina, Tešić, Živoslav Lj., Pešić, Mirjana B., "Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents." in Chemistry & Biodiversity, 19, no. 4 (2022):e202200069,
https://doi.org/10.1002/cbdv.202200069 . .

TY  - JOUR
AU  - Petrović, Jovana
AU  - Ângela, Fernandes
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel
AU  - Shekhar, Aditya
AU  - Glamočlija, Jasmina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4939
AB  - Edible mushrooms have been appreciated globally for their organoleptic, nutritional and chemical properties. In the present study, fruiting body
of Calocybe gambosa, wild growing in Serbia, has been chemically characterized (content of macronutrients, soluble sugars, tocopherols, fatty and or-
ganic acids) and its bioactive (antimicrobial and antioxidant) properties were evaluated. The results obtained suggest that this mushroom is a source
of carbohydrates and proteins (72 g/100 g dry weight (dw) and 16 g/100 g dw, respectively), with a low fat content (1.5 g/100 g dw). Sugar analysis
revealed the presence of trehalose (3.82 g/100 g dw) and mannitol (0.22 g/100 g dw). Tocopherol composition revealed the presence of α-tocopherol
(7.8 μg/100 g dw), while fatty acid analysis revealed the presence of 24 fatty acids with the prevalence of polyunsaturated fatty acids. Amongst organic
acids, oxalic, quinic, malic, citric and fumaric acid were detected (2.8 g/100 g dw, 1.45 g/100 g dw, 11.3 g/100 g dw, 1.3 g/100 g dw, 0.08 g/100 g dw,
respectively). Comprehensive antioxidant activity analysis (reducing power, DPPH radical scavenging activity, β-carotene/linoleate and TBARS assays)
indicate that the mushroom is a perspective antioxidant source, whereas its antimicrobial potential turned out to be moderate. Nevertheless, at a sub-
-inhibitory level, the methanolic extract disrupted cell-to-cell communication using Pseudomonas aeruginosa PAO1 as a model system. Finally, enrich-
ment of oatmeal cookies with C. gambosa flakes not only improved their nutritional value, but was praised among the participants in sensory evaluation
test, indicating that along with results of chemical composition and biological activity, this mushroom has a potential to be regarded as functional food.
PB  - Olsztyn :  Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
T2  - Polish Journal of Food and Nutrition Sciences
T1  - A Step Forward Towards Exploring Nutritional and Biological Potential of Mushrooms: A Case Study of Calocybe gambosa (Fr.) Donk Wild Growing in Serbia
IS  - 1
VL  - 72
DO  - 10.31883/pjfns/144836
SP  - 17
EP  - 26
ER  - 

@article{
author = "Petrović, Jovana and Ângela, Fernandes and Stojković, Dejan and Soković, Marina and Barros, Lillian and Ferreira, Isabel and Shekhar, Aditya and Glamočlija, Jasmina",
year = "2022",
abstract = "Edible mushrooms have been appreciated globally for their organoleptic, nutritional and chemical properties. In the present study, fruiting body
of Calocybe gambosa, wild growing in Serbia, has been chemically characterized (content of macronutrients, soluble sugars, tocopherols, fatty and or-
ganic acids) and its bioactive (antimicrobial and antioxidant) properties were evaluated. The results obtained suggest that this mushroom is a source
of carbohydrates and proteins (72 g/100 g dry weight (dw) and 16 g/100 g dw, respectively), with a low fat content (1.5 g/100 g dw). Sugar analysis
revealed the presence of trehalose (3.82 g/100 g dw) and mannitol (0.22 g/100 g dw). Tocopherol composition revealed the presence of α-tocopherol
(7.8 μg/100 g dw), while fatty acid analysis revealed the presence of 24 fatty acids with the prevalence of polyunsaturated fatty acids. Amongst organic
acids, oxalic, quinic, malic, citric and fumaric acid were detected (2.8 g/100 g dw, 1.45 g/100 g dw, 11.3 g/100 g dw, 1.3 g/100 g dw, 0.08 g/100 g dw,
respectively). Comprehensive antioxidant activity analysis (reducing power, DPPH radical scavenging activity, β-carotene/linoleate and TBARS assays)
indicate that the mushroom is a perspective antioxidant source, whereas its antimicrobial potential turned out to be moderate. Nevertheless, at a sub-
-inhibitory level, the methanolic extract disrupted cell-to-cell communication using Pseudomonas aeruginosa PAO1 as a model system. Finally, enrich-
ment of oatmeal cookies with C. gambosa flakes not only improved their nutritional value, but was praised among the participants in sensory evaluation
test, indicating that along with results of chemical composition and biological activity, this mushroom has a potential to be regarded as functional food.",
publisher = "Olsztyn :  Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences",
journal = "Polish Journal of Food and Nutrition Sciences",
title = "A Step Forward Towards Exploring Nutritional and Biological Potential of Mushrooms: A Case Study of Calocybe gambosa (Fr.) Donk Wild Growing in Serbia",
number = "1",
volume = "72",
doi = "10.31883/pjfns/144836",
pages = "17-26"
}

Petrović, J., Ângela, F., Stojković, D., Soković, M., Barros, L., Ferreira, I., Shekhar, A.,& Glamočlija, J.. (2022). A Step Forward Towards Exploring Nutritional and Biological Potential of Mushrooms: A Case Study of Calocybe gambosa (Fr.) Donk Wild Growing in Serbia. in Polish Journal of Food and Nutrition Sciences
Olsztyn :  Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences., 72(1), 17-26.
https://doi.org/10.31883/pjfns/144836

Petrović J, Ângela F, Stojković D, Soković M, Barros L, Ferreira I, Shekhar A, Glamočlija J. A Step Forward Towards Exploring Nutritional and Biological Potential of Mushrooms: A Case Study of Calocybe gambosa (Fr.) Donk Wild Growing in Serbia. in Polish Journal of Food and Nutrition Sciences. 2022;72(1):17-26.
doi:10.31883/pjfns/144836 .

Petrović, Jovana, Ângela, Fernandes, Stojković, Dejan, Soković, Marina, Barros, Lillian, Ferreira, Isabel, Shekhar, Aditya, Glamočlija, Jasmina, "A Step Forward Towards Exploring Nutritional and Biological Potential of Mushrooms: A Case Study of Calocybe gambosa (Fr.) Donk Wild Growing in Serbia" in Polish Journal of Food and Nutrition Sciences, 72, no. 1 (2022):17-26,
https://doi.org/10.31883/pjfns/144836 . .

TY  - JOUR
AU  - López-Hortas, L.
AU  - Caleja, C.
AU  - Pinela, J.
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Ferreira, I C F R
AU  - Torres, M D
AU  - Domínguez, H.
AU  - Pereira, E.
AU  - Barros, L.
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0308814622004125
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4861
AB  - Dehydration of the edible seaweed Chondrus crispus was performed by freeze-drying, conventional oven-drying and emerging microwave hydrodiffusion and gravity (MHG). In this work, the drying kinetics and modelling, estimating specific energy consumption and environmental impact of distinct processes were tested. Color and microstructural features of the dried macroalgae were also evaluated, as well as their nutritive characterization, chemical profile and bioactive potential (antioxidant and antimicrobial activities). Moreover, collected liquid phases from both the defrosted and MHG treated samples were also characterized. All methodologies provided solid phases with an adequate final moisture content. MHG significantly reduced the needed time, specific energy consumption and environmental impact, providing C. crispus with intermediate color and histological structure characteristics. Overall, this trend was also defined to tested chemical parameters and bioactivities. MHG provided aqueous extracts with potential bioactive compounds from this red alga, increasing the efficiency of this drying method.
PB  - Elsevier Ltd
T2  - Food Chemistry
T1  - Comparative evaluation of physicochemical profile and bioactive properties of red edible seaweed Chondrus crispus subjected to different drying methods.
VL  - 383
DO  - 10.1016/j.foodchem.2022.132450
SP  - 132450
ER  - 

@article{
author = "López-Hortas, L. and Caleja, C. and Pinela, J. and Petrović, Jovana and Soković, Marina and Ferreira, I C F R and Torres, M D and Domínguez, H. and Pereira, E. and Barros, L.",
year = "2022",
abstract = "Dehydration of the edible seaweed Chondrus crispus was performed by freeze-drying, conventional oven-drying and emerging microwave hydrodiffusion and gravity (MHG). In this work, the drying kinetics and modelling, estimating specific energy consumption and environmental impact of distinct processes were tested. Color and microstructural features of the dried macroalgae were also evaluated, as well as their nutritive characterization, chemical profile and bioactive potential (antioxidant and antimicrobial activities). Moreover, collected liquid phases from both the defrosted and MHG treated samples were also characterized. All methodologies provided solid phases with an adequate final moisture content. MHG significantly reduced the needed time, specific energy consumption and environmental impact, providing C. crispus with intermediate color and histological structure characteristics. Overall, this trend was also defined to tested chemical parameters and bioactivities. MHG provided aqueous extracts with potential bioactive compounds from this red alga, increasing the efficiency of this drying method.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry",
title = "Comparative evaluation of physicochemical profile and bioactive properties of red edible seaweed Chondrus crispus subjected to different drying methods.",
volume = "383",
doi = "10.1016/j.foodchem.2022.132450",
pages = "132450"
}

López-Hortas, L., Caleja, C., Pinela, J., Petrović, J., Soković, M., Ferreira, I. C. F. R., Torres, M. D., Domínguez, H., Pereira, E.,& Barros, L.. (2022). Comparative evaluation of physicochemical profile and bioactive properties of red edible seaweed Chondrus crispus subjected to different drying methods.. in Food Chemistry
Elsevier Ltd., 383, 132450.
https://doi.org/10.1016/j.foodchem.2022.132450

López-Hortas L, Caleja C, Pinela J, Petrović J, Soković M, Ferreira ICFR, Torres MD, Domínguez H, Pereira E, Barros L. Comparative evaluation of physicochemical profile and bioactive properties of red edible seaweed Chondrus crispus subjected to different drying methods.. in Food Chemistry. 2022;383:132450.
doi:10.1016/j.foodchem.2022.132450 .

López-Hortas, L., Caleja, C., Pinela, J., Petrović, Jovana, Soković, Marina, Ferreira, I C F R, Torres, M D, Domínguez, H., Pereira, E., Barros, L., "Comparative evaluation of physicochemical profile and bioactive properties of red edible seaweed Chondrus crispus subjected to different drying methods." in Food Chemistry, 383 (2022):132450,
https://doi.org/10.1016/j.foodchem.2022.132450 . .

TY  - JOUR
AU  - Petrović, Jovana
AU  - Kovalenko, Vitaly
AU  - Svirid, Anastasia
AU  - Stojković, Dejan
AU  - Ivanov, Marija
AU  - Kostić, Marina
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0022286021021190
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4744
AB  - Naturally occurring terpene core compounds have been used extensively in both pharmaceutical and cosmetic industry. However, since chirality of these compounds has profound influence on the level of their bioactivity, the aim of the present study was to assess broad spectrum activities of four possible stereoisomers of monoterpene alcohol verbenol and both enantiomers of monoterpene ketone verbenone. Antimicrobial activity was evaluated against different bacterial and fungal strains of clinical relevance. Tested compounds exhibited antibacterial activity within MICs range of 0.6–2.5 mg mL−1, antifungal activity within MICs range of 0.08–0.6 mg mL−1, and anticandidal activity with MICs 0.3–1.2 mg mL−1. While there was no difference in antibacterial activity between the enantiomers of verbenone, this was not the case with tested verbenol enantiomers. Furthermore, the compounds expressed no cytotoxicity to human keratinocyte cell line HaCaT at the tested concentrations. Interestingly, compounds contributed to the downregulation of interleukin IL-6 levels in HaCaT cells exposed to bacterial agent Staphylococcus lugdunensis. According to our knowledge, this study is the first that comparatively describes biological activities of stereoisomers of verbenol and enantiomers of verbenone. Current findings highlighted the importance of stereochemical purity of monoterpenoids in relation to their unique biological features.
T2  - Journal of Molecular Structure
T1  - Individual stereoisomers of verbenol and verbenone express bioactive features
VL  - 1251
DO  - 10.1016/j.molstruc.2021.131999
SP  - 131999
ER  - 

@article{
author = "Petrović, Jovana and Kovalenko, Vitaly and Svirid, Anastasia and Stojković, Dejan and Ivanov, Marija and Kostić, Marina",
year = "2022",
abstract = "Naturally occurring terpene core compounds have been used extensively in both pharmaceutical and cosmetic industry. However, since chirality of these compounds has profound influence on the level of their bioactivity, the aim of the present study was to assess broad spectrum activities of four possible stereoisomers of monoterpene alcohol verbenol and both enantiomers of monoterpene ketone verbenone. Antimicrobial activity was evaluated against different bacterial and fungal strains of clinical relevance. Tested compounds exhibited antibacterial activity within MICs range of 0.6–2.5 mg mL−1, antifungal activity within MICs range of 0.08–0.6 mg mL−1, and anticandidal activity with MICs 0.3–1.2 mg mL−1. While there was no difference in antibacterial activity between the enantiomers of verbenone, this was not the case with tested verbenol enantiomers. Furthermore, the compounds expressed no cytotoxicity to human keratinocyte cell line HaCaT at the tested concentrations. Interestingly, compounds contributed to the downregulation of interleukin IL-6 levels in HaCaT cells exposed to bacterial agent Staphylococcus lugdunensis. According to our knowledge, this study is the first that comparatively describes biological activities of stereoisomers of verbenol and enantiomers of verbenone. Current findings highlighted the importance of stereochemical purity of monoterpenoids in relation to their unique biological features.",
journal = "Journal of Molecular Structure",
title = "Individual stereoisomers of verbenol and verbenone express bioactive features",
volume = "1251",
doi = "10.1016/j.molstruc.2021.131999",
pages = "131999"
}

Petrović, J., Kovalenko, V., Svirid, A., Stojković, D., Ivanov, M.,& Kostić, M.. (2022). Individual stereoisomers of verbenol and verbenone express bioactive features. in Journal of Molecular Structure, 1251, 131999.
https://doi.org/10.1016/j.molstruc.2021.131999

Petrović J, Kovalenko V, Svirid A, Stojković D, Ivanov M, Kostić M. Individual stereoisomers of verbenol and verbenone express bioactive features. in Journal of Molecular Structure. 2022;1251:131999.
doi:10.1016/j.molstruc.2021.131999 .

Petrović, Jovana, Kovalenko, Vitaly, Svirid, Anastasia, Stojković, Dejan, Ivanov, Marija, Kostić, Marina, "Individual stereoisomers of verbenol and verbenone express bioactive features" in Journal of Molecular Structure, 1251 (2022):131999,
https://doi.org/10.1016/j.molstruc.2021.131999 . .

TY  - CHAP
AU  - Petrović, Jovana
AU  - Kostić, Marina
AU  - Stojković, Dejan
AU  - Glamočlija, Jasmina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5808
AB  - Fungi are an untapped resource with both nutritive and medicinal potentials,
that have been undervalued and underexplored until recently, but they are now
increasingly utilized in the pharmaceutical and food industries. With the re evaluated concept of using food in prophylaxis of diseases and as therapeutic
agents, contemporary research has been strongly focused on this area, leading
to scientifically supported facts. Since the nutritive properties of edible
mushrooms have been praised in scientific research, an up-to-date literature
review on these properties will be presented so as to evaluate the novel prospects and possible applications of edible mushrooms in the food industry
PB  - London, UK: The Royal Society of Chemistry
T2  - Edible Fungi: Chemical Composition, Nutrition and Health Effects
T1  - Applications of Mushrooms in the food industry
DO  - 10.1039/9781839167522-00359
SP  - 359
EP  - 382
ER  - 

@inbook{
author = "Petrović, Jovana and Kostić, Marina and Stojković, Dejan and Glamočlija, Jasmina",
year = "2022",
abstract = "Fungi are an untapped resource with both nutritive and medicinal potentials,
that have been undervalued and underexplored until recently, but they are now
increasingly utilized in the pharmaceutical and food industries. With the re evaluated concept of using food in prophylaxis of diseases and as therapeutic
agents, contemporary research has been strongly focused on this area, leading
to scientifically supported facts. Since the nutritive properties of edible
mushrooms have been praised in scientific research, an up-to-date literature
review on these properties will be presented so as to evaluate the novel prospects and possible applications of edible mushrooms in the food industry",
publisher = "London, UK: The Royal Society of Chemistry",
journal = "Edible Fungi: Chemical Composition, Nutrition and Health Effects",
booktitle = "Applications of Mushrooms in the food industry",
doi = "10.1039/9781839167522-00359",
pages = "359-382"
}

Petrović, J., Kostić, M., Stojković, D.,& Glamočlija, J.. (2022). Applications of Mushrooms in the food industry. in Edible Fungi: Chemical Composition, Nutrition and Health Effects
London, UK: The Royal Society of Chemistry., 359-382.
https://doi.org/10.1039/9781839167522-00359

Petrović J, Kostić M, Stojković D, Glamočlija J. Applications of Mushrooms in the food industry. in Edible Fungi: Chemical Composition, Nutrition and Health Effects. 2022;:359-382.
doi:10.1039/9781839167522-00359 .

Petrović, Jovana, Kostić, Marina, Stojković, Dejan, Glamočlija, Jasmina, "Applications of Mushrooms in the food industry" in Edible Fungi: Chemical Composition, Nutrition and Health Effects (2022):359-382,
https://doi.org/10.1039/9781839167522-00359 . .

TY  - CONF
AU  - Ivanov, Marija
AU  - Carević, Tamara
AU  - Kostić, Marina
AU  - Ćirić, Ana
AU  - Petrović, Jovana
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5751
AB  - Terpenoids are the most complex class of natural products with over 80000 identified representatives found in plants, microorganisms, insects, and marine invertebrates. Their anticandidal potential, especially impact on fungal virulence is, however, not fully explored yet. We aimed to investigate anticandidal, antibiofilm, and antihyphal properties of selected terpenoids (borneol, thymol, geraniol, and carvacrol). The strongest antifungal potential towards Candida albicans strains could be noticed for borneol (lowest minimal inhibitory concentration, MIC, 0.06 mg/mL) followed by thymol (lowest MIC 0.12 mg/mL). On the other hand, MIC of geraniol was up to 8 mg/mL. Non-albicans Candida strains were most susceptible to thymol (MIC 0.12 mg/mL towards C. krusei H1/16) and borneol (MIC 0.12 mg/mL towards C. parapsilosis ATCC 22019). When the terpenoids were applied in their previously determined minimal inhibitory concentrations the strongest reducing impact on C. albicans ATCC 10231 biofilm forming ability was observed for carvacrol (60% inhibition), followed by geraniol (56% inhibition). Lower concentration of terpenoids (0.5 MIC) led to similar impairment of biofilm formation – the strongest impact was noticed for geraniol (54% inhibition), followed by carvacrol (47% inhibition) as determined by crystal violet assay. MTT antibiofilm assay highlighted great potential of carvacrol and geraniol to reduce viability of Candida cells in biofilms. Inhibition of hyphal formation could be noticed only upon application of carvacrol (14.29% of hyphal cells compared to the untreated control with 50.67% hyphae) suggesting wide antivirulence potential exhibited by this molecule. Other compounds explored in this study have displayed high MIC values (geraniol), low antibiofilm potential (thymol, borneol) or lack of antihyphal properties (all except carvacrol). Potential of carvacrol to reduce fungal virulence should be further studied on molecular level and in in vivo assays.
PB  - Belgrade: University of Belgrade, Faculty of Agriculture
C3  - Book of abstracts: 1st European Symposium on Phytochemicals in Medicine and Food, 1-EuSPMF; 2022 Sep 7-9; Belgrade, Serbia
T1  - Selected terpenoids as obstructers of Candida virulence
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5751
ER  - 

@conference{
author = "Ivanov, Marija and Carević, Tamara and Kostić, Marina and Ćirić, Ana and Petrović, Jovana and Glamočlija, Jasmina and Soković, Marina",
year = "2022",
abstract = "Terpenoids are the most complex class of natural products with over 80000 identified representatives found in plants, microorganisms, insects, and marine invertebrates. Their anticandidal potential, especially impact on fungal virulence is, however, not fully explored yet. We aimed to investigate anticandidal, antibiofilm, and antihyphal properties of selected terpenoids (borneol, thymol, geraniol, and carvacrol). The strongest antifungal potential towards Candida albicans strains could be noticed for borneol (lowest minimal inhibitory concentration, MIC, 0.06 mg/mL) followed by thymol (lowest MIC 0.12 mg/mL). On the other hand, MIC of geraniol was up to 8 mg/mL. Non-albicans Candida strains were most susceptible to thymol (MIC 0.12 mg/mL towards C. krusei H1/16) and borneol (MIC 0.12 mg/mL towards C. parapsilosis ATCC 22019). When the terpenoids were applied in their previously determined minimal inhibitory concentrations the strongest reducing impact on C. albicans ATCC 10231 biofilm forming ability was observed for carvacrol (60% inhibition), followed by geraniol (56% inhibition). Lower concentration of terpenoids (0.5 MIC) led to similar impairment of biofilm formation – the strongest impact was noticed for geraniol (54% inhibition), followed by carvacrol (47% inhibition) as determined by crystal violet assay. MTT antibiofilm assay highlighted great potential of carvacrol and geraniol to reduce viability of Candida cells in biofilms. Inhibition of hyphal formation could be noticed only upon application of carvacrol (14.29% of hyphal cells compared to the untreated control with 50.67% hyphae) suggesting wide antivirulence potential exhibited by this molecule. Other compounds explored in this study have displayed high MIC values (geraniol), low antibiofilm potential (thymol, borneol) or lack of antihyphal properties (all except carvacrol). Potential of carvacrol to reduce fungal virulence should be further studied on molecular level and in in vivo assays.",
publisher = "Belgrade: University of Belgrade, Faculty of Agriculture",
journal = "Book of abstracts: 1st European Symposium on Phytochemicals in Medicine and Food, 1-EuSPMF; 2022 Sep 7-9; Belgrade, Serbia",
title = "Selected terpenoids as obstructers of Candida virulence",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5751"
}

Ivanov, M., Carević, T., Kostić, M., Ćirić, A., Petrović, J., Glamočlija, J.,& Soković, M.. (2022). Selected terpenoids as obstructers of Candida virulence. in Book of abstracts: 1st European Symposium on Phytochemicals in Medicine and Food, 1-EuSPMF; 2022 Sep 7-9; Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Agriculture..
https://hdl.handle.net/21.15107/rcub_ibiss_5751

Ivanov M, Carević T, Kostić M, Ćirić A, Petrović J, Glamočlija J, Soković M. Selected terpenoids as obstructers of Candida virulence. in Book of abstracts: 1st European Symposium on Phytochemicals in Medicine and Food, 1-EuSPMF; 2022 Sep 7-9; Belgrade, Serbia. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5751 .

Ivanov, Marija, Carević, Tamara, Kostić, Marina, Ćirić, Ana, Petrović, Jovana, Glamočlija, Jasmina, Soković, Marina, "Selected terpenoids as obstructers of Candida virulence" in Book of abstracts: 1st European Symposium on Phytochemicals in Medicine and Food, 1-EuSPMF; 2022 Sep 7-9; Belgrade, Serbia (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5751 .

TY  - CONF
AU  - Stojković, Dejan
AU  - Kostić, Marina
AU  - Petrović, Jovana
AU  - Ćirić, Ana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5154
AB  - Fungal infections are a serious health related burden. The increasing and non-selective use of commercial antimicrobial therapeutics, due to numerous infections and diseases, causes an increase in the resistance of pathogenic microorganisms to synthetic antimicrobial drugs. Although a large number of plant species used in traditional medicine, have been well phytochemically and pharmacologically investigated and therefore recognized in modern medicine, large number still remains unexplored.
The aim of this study was to examine the antifungal potential of aqueous and ethanolic extracts of the plant golden marguerite - Cota tinctoria on the certain yeasts and dermatomycetes. The aerial parts of the Cota tinctoria plant were used for the extract preparation. The microdilution method was used to examine antifungal activity. The minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of the tested extracts were determined. The following fungal species were used: Candida albicans (clinical isolate), Candida albicans (ATCC 10231), Candida krusei (clinical isolate), Microsporum gypseum (clinical isolate), Trichophyton rubrum (ATCC 28188) and Trichophyton mentagrophytes (clinical isolate).
Aqueous extracts, except for the inhibitory effect on C. albicans, had no effect on the tested yeasts and dermatomycetes. Ethanol extracts showed different antifungal activity. The most sensitive species were: T. mentagrophytes (MIC 0.50 mg/mL, MFC 1.00 mg/mL) and T. rubrum (MIC 0.75 mg/mL, MFC 2.00 mg/mL), while the most resistant species was C. krusei (MIC 8.00 mg/ml). Commercial antifungals, ketoconazole (MIC 0.003 - 0.05 mg/mL) and fluconazole (MIC 0.006 - 0.05 mg/mL) showed better antifungal activity compared to the tested extracts.
The results of this investigation showed good antifungal characteristics of C. tinctoria plant extracts and may have potential application as an adjunct in the treatment of superficial infections caused by certain micromycetes.
PB  - Novi Sad: Matica srpska
C3  - Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia
T1  - Investigation of antifungal potential of aqueous and ethanolic extracts of the plant Cota tinctoria on selected isolates of yeasts and dermatomycetes
SP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5154
ER  - 

@conference{
author = "Stojković, Dejan and Kostić, Marina and Petrović, Jovana and Ćirić, Ana",
year = "2022",
abstract = "Fungal infections are a serious health related burden. The increasing and non-selective use of commercial antimicrobial therapeutics, due to numerous infections and diseases, causes an increase in the resistance of pathogenic microorganisms to synthetic antimicrobial drugs. Although a large number of plant species used in traditional medicine, have been well phytochemically and pharmacologically investigated and therefore recognized in modern medicine, large number still remains unexplored.
The aim of this study was to examine the antifungal potential of aqueous and ethanolic extracts of the plant golden marguerite - Cota tinctoria on the certain yeasts and dermatomycetes. The aerial parts of the Cota tinctoria plant were used for the extract preparation. The microdilution method was used to examine antifungal activity. The minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of the tested extracts were determined. The following fungal species were used: Candida albicans (clinical isolate), Candida albicans (ATCC 10231), Candida krusei (clinical isolate), Microsporum gypseum (clinical isolate), Trichophyton rubrum (ATCC 28188) and Trichophyton mentagrophytes (clinical isolate).
Aqueous extracts, except for the inhibitory effect on C. albicans, had no effect on the tested yeasts and dermatomycetes. Ethanol extracts showed different antifungal activity. The most sensitive species were: T. mentagrophytes (MIC 0.50 mg/mL, MFC 1.00 mg/mL) and T. rubrum (MIC 0.75 mg/mL, MFC 2.00 mg/mL), while the most resistant species was C. krusei (MIC 8.00 mg/ml). Commercial antifungals, ketoconazole (MIC 0.003 - 0.05 mg/mL) and fluconazole (MIC 0.006 - 0.05 mg/mL) showed better antifungal activity compared to the tested extracts.
The results of this investigation showed good antifungal characteristics of C. tinctoria plant extracts and may have potential application as an adjunct in the treatment of superficial infections caused by certain micromycetes.",
publisher = "Novi Sad: Matica srpska",
journal = "Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia",
title = "Investigation of antifungal potential of aqueous and ethanolic extracts of the plant Cota tinctoria on selected isolates of yeasts and dermatomycetes",
pages = "45",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5154"
}

Stojković, D., Kostić, M., Petrović, J.,& Ćirić, A.. (2022). Investigation of antifungal potential of aqueous and ethanolic extracts of the plant Cota tinctoria on selected isolates of yeasts and dermatomycetes. in Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia
Novi Sad: Matica srpska., 45.
https://hdl.handle.net/21.15107/rcub_ibiss_5154

Stojković D, Kostić M, Petrović J, Ćirić A. Investigation of antifungal potential of aqueous and ethanolic extracts of the plant Cota tinctoria on selected isolates of yeasts and dermatomycetes. in Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia. 2022;:45.
https://hdl.handle.net/21.15107/rcub_ibiss_5154 .

Stojković, Dejan, Kostić, Marina, Petrović, Jovana, Ćirić, Ana, "Investigation of antifungal potential of aqueous and ethanolic extracts of the plant Cota tinctoria on selected isolates of yeasts and dermatomycetes" in Book of abstracts: The 7th international scientific meeting "Mycology, mycotoxicology, and mycoses"; 2022 Jun 2-3; Novi Sad, Serbia (2022):45,
https://hdl.handle.net/21.15107/rcub_ibiss_5154 .

TY  - CONF
AU  - Añibarro-Ortega, Mikel
AU  - Pinela, José
AU  - Petrović, Jovana
AU  - Prieto, Miguel Angel
AU  - Soković, Marina
AU  - Ferreira, Isabel
AU  - Simal-Gandara, Jesus
AU  - Barros, Lillian
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6691
AB  - European consumers have a growing interest in new fruits and flavours, which has promoted the production and commercialisation of exotic fruits such as goldenberry (Physalis peruviana L., Fam. Solanaceae). Colombia and South Africa are currently the main producers of this fruit, but it can be cultivated in almost all the highlands of the tropics and in several parts of the subtropics, given its ability to adapt to a wide range of agroecological conditions [1]. Consumer demand for this small orange berry has also been driven by its nutritional value and health-promoting effects [2]. Although there have been different studies about this fruit, the available information is still scarce. Furthermore, the fruit quality attributes can vary depending on the agricultural practices used and the edaphoclimatic conditions of the growing sites. Therefore, this study was carried out to evaluate the nutritional composition and in vitro antioxidant and antimicrobial activity of goldenberry cultivated in the northeast region of Portugal. Goldenberry samples were lyophilised and analysed to determine their proximate composition (moisture, protein, fat, ash, dietary fibre, and carbohydrates), following official methods of food analysis [3]. Their profiles of sugars, organic acids, fatty acids, and tocopherols were characterised by standardised chromatographic methods [3]. The powdered goldenberry sample was also prepared in a hydroethanolic extract used for the evaluation of antioxidant activity (by oxidative haemolysis and lipid peroxidation inhibition assays) and antimicrobial effects against foodborne bacteria and fungi (by serial microdilution methods) [3]. The nutritional analysis revealed high levels of carbohydrates (manly fructose and glucose) and fibre and a lipid fraction consisting mainly of polyunsaturated fatty acids. Citric and ascorbic acids were detected in high amounts, as well as the four tocopherol isoforms. The hydroethanolic berry extract showed capacity for inhibiting haemolytic oxidation and lipid peroxidation, antibacterial effects against Staphylococcus aureus and Bacillus cereus, and antifungal activity against Aspergillus and Penicillium strains. Overall, this work highlighted the nutritional value of goldenberry, which has been noted as a functional fruit with a growing presence in the Portuguese market.
PB  - Basel: MDPI
C3  - The 2nd International Electronic Conference on Foods—“Future Foods and Food Technologies for a Sustainable World; 2021 Oct 15-30; OnlineBiology and Life Sciences Forum
T1  - Nutritional Composition and Biological Activity of Goldenberry (Physalis peruviana L.): An Emerging Fruit Crop in Portugal.
IS  - 3
VL  - 6
DO  - 10.3390/Foods2021-10949
ER  - 

@conference{
author = "Añibarro-Ortega, Mikel and Pinela, José and Petrović, Jovana and Prieto, Miguel Angel and Soković, Marina and Ferreira, Isabel and Simal-Gandara, Jesus and Barros, Lillian",
year = "2021",
abstract = "European consumers have a growing interest in new fruits and flavours, which has promoted the production and commercialisation of exotic fruits such as goldenberry (Physalis peruviana L., Fam. Solanaceae). Colombia and South Africa are currently the main producers of this fruit, but it can be cultivated in almost all the highlands of the tropics and in several parts of the subtropics, given its ability to adapt to a wide range of agroecological conditions [1]. Consumer demand for this small orange berry has also been driven by its nutritional value and health-promoting effects [2]. Although there have been different studies about this fruit, the available information is still scarce. Furthermore, the fruit quality attributes can vary depending on the agricultural practices used and the edaphoclimatic conditions of the growing sites. Therefore, this study was carried out to evaluate the nutritional composition and in vitro antioxidant and antimicrobial activity of goldenberry cultivated in the northeast region of Portugal. Goldenberry samples were lyophilised and analysed to determine their proximate composition (moisture, protein, fat, ash, dietary fibre, and carbohydrates), following official methods of food analysis [3]. Their profiles of sugars, organic acids, fatty acids, and tocopherols were characterised by standardised chromatographic methods [3]. The powdered goldenberry sample was also prepared in a hydroethanolic extract used for the evaluation of antioxidant activity (by oxidative haemolysis and lipid peroxidation inhibition assays) and antimicrobial effects against foodborne bacteria and fungi (by serial microdilution methods) [3]. The nutritional analysis revealed high levels of carbohydrates (manly fructose and glucose) and fibre and a lipid fraction consisting mainly of polyunsaturated fatty acids. Citric and ascorbic acids were detected in high amounts, as well as the four tocopherol isoforms. The hydroethanolic berry extract showed capacity for inhibiting haemolytic oxidation and lipid peroxidation, antibacterial effects against Staphylococcus aureus and Bacillus cereus, and antifungal activity against Aspergillus and Penicillium strains. Overall, this work highlighted the nutritional value of goldenberry, which has been noted as a functional fruit with a growing presence in the Portuguese market.",
publisher = "Basel: MDPI",
journal = "The 2nd International Electronic Conference on Foods—“Future Foods and Food Technologies for a Sustainable World; 2021 Oct 15-30; OnlineBiology and Life Sciences Forum",
title = "Nutritional Composition and Biological Activity of Goldenberry (Physalis peruviana L.): An Emerging Fruit Crop in Portugal.",
number = "3",
volume = "6",
doi = "10.3390/Foods2021-10949"
}

Añibarro-Ortega, M., Pinela, J., Petrović, J., Prieto, M. A., Soković, M., Ferreira, I., Simal-Gandara, J.,& Barros, L.. (2021). Nutritional Composition and Biological Activity of Goldenberry (Physalis peruviana L.): An Emerging Fruit Crop in Portugal.. in The 2nd International Electronic Conference on Foods—“Future Foods and Food Technologies for a Sustainable World; 2021 Oct 15-30; OnlineBiology and Life Sciences Forum
Basel: MDPI., 6(3).
https://doi.org/10.3390/Foods2021-10949

Añibarro-Ortega M, Pinela J, Petrović J, Prieto MA, Soković M, Ferreira I, Simal-Gandara J, Barros L. Nutritional Composition and Biological Activity of Goldenberry (Physalis peruviana L.): An Emerging Fruit Crop in Portugal.. in The 2nd International Electronic Conference on Foods—“Future Foods and Food Technologies for a Sustainable World; 2021 Oct 15-30; OnlineBiology and Life Sciences Forum. 2021;6(3).
doi:10.3390/Foods2021-10949 .

Añibarro-Ortega, Mikel, Pinela, José, Petrović, Jovana, Prieto, Miguel Angel, Soković, Marina, Ferreira, Isabel, Simal-Gandara, Jesus, Barros, Lillian, "Nutritional Composition and Biological Activity of Goldenberry (Physalis peruviana L.): An Emerging Fruit Crop in Portugal." in The 2nd International Electronic Conference on Foods—“Future Foods and Food Technologies for a Sustainable World; 2021 Oct 15-30; OnlineBiology and Life Sciences Forum, 6, no. 3 (2021),
https://doi.org/10.3390/Foods2021-10949 . .

TY  - CONF
AU  - Popović Minić, Dušanka
AU  - Milinčić, Danijel
AU  - Rac, Vladislav
AU  - Vidović, Bojana
AU  - Soković, Marina
AU  - Petrović, Jovana
AU  - Pešić, Mirjana
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6573
AB  - In recent years, the manufacturing of functional food has gained a lot of attention. The most recent 
studies indicate that bovine milk proteins are suitable carriers for a whole range of bioactive 
substances, mainly because of their micellar structure.
The aim of our study was to examine the possibility of goat milk proteins (RM - raw skimmed milk 
and CN - casein fraction) to deliver A. blazei extracts (VA - hot water extract and GA - glucan 
extract) in order to use all the potential health benefits of this mushroom. Furthermore, the effect of 
thermal treatment (90⁰C, 10 min; TRM – thermally treated RM and TCN - thermally treated CN) on 
carrier properties was also evaluated. The mixture of different goat milk proteins and A. blazei
extracts were prepared and zeta-potential and particle size distribution were determined by a laser 
light-scattering particle size analyzer, using distilled water as a dispersant.
The best results were obtained with the RM and TRM mixtures. It can be concluded that changes in 
particle size and electric charge of the micellar surface occurring in goat milk after heating have a 
positive impact on carrier properties. The particle size of TRM mixtures increased by 13.3 to 15.7% 
compared to the size of TRM particles, whereas the zeta-potential of TRM/VA increased for 6.6% 
compared to that of TRM. TCNs did not follow this trend. The measurement of CNs particle size 
distribution showed the presence of significantly different sizes (coefficient of variation >30%), 
whereas the zeta-potential measurements were stable. These results imply that the measurements of 
zeta-potential cannot be interpreted without the particle size data.
PB  - Belgrade: University of Belgrad
C3  - Book of abstracts: 2nd UNIFood International Conference: UNIFood2021 Conferenece; 2021 Sep 24-25; Belgrade, Serbia
T1  - Zeta-potential and particle size of functional additives based on goat milk proteins and Agaricus blazei Murill extracts.
SP  - 145
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6573
ER  - 

@conference{
author = "Popović Minić, Dušanka and Milinčić, Danijel and Rac, Vladislav and Vidović, Bojana and Soković, Marina and Petrović, Jovana and Pešić, Mirjana",
year = "2021",
abstract = "In recent years, the manufacturing of functional food has gained a lot of attention. The most recent 
studies indicate that bovine milk proteins are suitable carriers for a whole range of bioactive 
substances, mainly because of their micellar structure.
The aim of our study was to examine the possibility of goat milk proteins (RM - raw skimmed milk 
and CN - casein fraction) to deliver A. blazei extracts (VA - hot water extract and GA - glucan 
extract) in order to use all the potential health benefits of this mushroom. Furthermore, the effect of 
thermal treatment (90⁰C, 10 min; TRM – thermally treated RM and TCN - thermally treated CN) on 
carrier properties was also evaluated. The mixture of different goat milk proteins and A. blazei
extracts were prepared and zeta-potential and particle size distribution were determined by a laser 
light-scattering particle size analyzer, using distilled water as a dispersant.
The best results were obtained with the RM and TRM mixtures. It can be concluded that changes in 
particle size and electric charge of the micellar surface occurring in goat milk after heating have a 
positive impact on carrier properties. The particle size of TRM mixtures increased by 13.3 to 15.7% 
compared to the size of TRM particles, whereas the zeta-potential of TRM/VA increased for 6.6% 
compared to that of TRM. TCNs did not follow this trend. The measurement of CNs particle size 
distribution showed the presence of significantly different sizes (coefficient of variation >30%), 
whereas the zeta-potential measurements were stable. These results imply that the measurements of 
zeta-potential cannot be interpreted without the particle size data.",
publisher = "Belgrade: University of Belgrad",
journal = "Book of abstracts: 2nd UNIFood International Conference: UNIFood2021 Conferenece; 2021 Sep 24-25; Belgrade, Serbia",
title = "Zeta-potential and particle size of functional additives based on goat milk proteins and Agaricus blazei Murill extracts.",
pages = "145",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6573"
}

Popović Minić, D., Milinčić, D., Rac, V., Vidović, B., Soković, M., Petrović, J.,& Pešić, M.. (2021). Zeta-potential and particle size of functional additives based on goat milk proteins and Agaricus blazei Murill extracts.. in Book of abstracts: 2nd UNIFood International Conference: UNIFood2021 Conferenece; 2021 Sep 24-25; Belgrade, Serbia
Belgrade: University of Belgrad., 145.
https://hdl.handle.net/21.15107/rcub_ibiss_6573

Popović Minić D, Milinčić D, Rac V, Vidović B, Soković M, Petrović J, Pešić M. Zeta-potential and particle size of functional additives based on goat milk proteins and Agaricus blazei Murill extracts.. in Book of abstracts: 2nd UNIFood International Conference: UNIFood2021 Conferenece; 2021 Sep 24-25; Belgrade, Serbia. 2021;:145.
https://hdl.handle.net/21.15107/rcub_ibiss_6573 .

Popović Minić, Dušanka, Milinčić, Danijel, Rac, Vladislav, Vidović, Bojana, Soković, Marina, Petrović, Jovana, Pešić, Mirjana, "Zeta-potential and particle size of functional additives based on goat milk proteins and Agaricus blazei Murill extracts." in Book of abstracts: 2nd UNIFood International Conference: UNIFood2021 Conferenece; 2021 Sep 24-25; Belgrade, Serbia (2021):145,
https://hdl.handle.net/21.15107/rcub_ibiss_6573 .

TY  - CONF
AU  - Albuquerque, Bianca
AU  - Inês Dias, Maria
AU  - Pereira, Carla
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - P.P. Oliveira, M.  Beatriz
AU  - C.F.C. Ferreira, Isabel
AU  - Barros, Lillian
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6574
AB  - Tropical fruits are widely appreciated for their sweet taste and generally known for their rich 
composition in bioactive compounds. However, the processing of this type of fruits can generate a large 
amount of non-edible and non-tradable by-products, consisting mainly in peels and seeds. These by products have already been described as having a greater amount of bioactive compounds than the 
edible parts; this fact has been increasing the interest of the scientific community as also of several 
industrial fields for the application of these by-products. 1
In this context, the rigid non-edible epicarp 
of Sicana odorifera (vell.) Naudin, a purple-black tropical fruit from Brazil, 2 was evaluated for the 
anthocyanin and non-anthocyanin phenolic composition by High-Performance Liquid Chromatography 
coupled to a diode-array detector and a mass spectrometer functioning by electrospray ionization 
(HPLC-DAD/ESI-MS). The bioactive potential was assessed thought several in vitro assays: the 
antioxidant activity was determined by thiobarbituric acid reactive substances assay (TBARS) and by the 
oxidative hemolysis inhibition assay (OxHLIA) and the antimicrobial activity was tested in four bacteria 
and four fungi strains using the microdilution method. 
S. odorifera epicarp hydroethanolic extract presented four phenolic compounds, namely two O glycosylated quercetin and kaempherol derivatives and two O-glycosylated anthocyanins, being the 
latest group the most abundant one with a total amount of 24±1 mg of anthocyanins/g of dry epicarp. 
Concerning its bioactive potential, S. odorifera hydroethanolic extract revealed considerable antioxidant 
activity (EC50 values of 48.2±0.5 and 27±1 µg/mL for TBARS and OxHLIA assays, respectively) and 
antimicrobial action against all bacteria and fungi strains evaluated (minimal inhibitory concentrations 
(MICs) ≤ 2.2 mg/mL). 
The results obtained allow to classify this tropical fruit epicarp as a potential source of bioactive 
compounds with great market value, since they can be applied in several industrial sectors, including 
the food and pharmaceutical industries.
PB  - Universidade da Madeira, Centro de Química da Madeira
C3  - XV Encontro de Química dos Alimentos; 2021 Sep 5-8; Madeira, Portugal
T1  - By-products of tropical fruits as sources of bioactive molecules: Sicana odorifera (vell.) Naudin epicarp case study.
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6574
ER  - 

@conference{
author = "Albuquerque, Bianca and Inês Dias, Maria and Pereira, Carla and Petrović, Jovana and Soković, Marina and P.P. Oliveira, M.  Beatriz and C.F.C. Ferreira, Isabel and Barros, Lillian",
year = "2021",
abstract = "Tropical fruits are widely appreciated for their sweet taste and generally known for their rich 
composition in bioactive compounds. However, the processing of this type of fruits can generate a large 
amount of non-edible and non-tradable by-products, consisting mainly in peels and seeds. These by products have already been described as having a greater amount of bioactive compounds than the 
edible parts; this fact has been increasing the interest of the scientific community as also of several 
industrial fields for the application of these by-products. 1
In this context, the rigid non-edible epicarp 
of Sicana odorifera (vell.) Naudin, a purple-black tropical fruit from Brazil, 2 was evaluated for the 
anthocyanin and non-anthocyanin phenolic composition by High-Performance Liquid Chromatography 
coupled to a diode-array detector and a mass spectrometer functioning by electrospray ionization 
(HPLC-DAD/ESI-MS). The bioactive potential was assessed thought several in vitro assays: the 
antioxidant activity was determined by thiobarbituric acid reactive substances assay (TBARS) and by the 
oxidative hemolysis inhibition assay (OxHLIA) and the antimicrobial activity was tested in four bacteria 
and four fungi strains using the microdilution method. 
S. odorifera epicarp hydroethanolic extract presented four phenolic compounds, namely two O glycosylated quercetin and kaempherol derivatives and two O-glycosylated anthocyanins, being the 
latest group the most abundant one with a total amount of 24±1 mg of anthocyanins/g of dry epicarp. 
Concerning its bioactive potential, S. odorifera hydroethanolic extract revealed considerable antioxidant 
activity (EC50 values of 48.2±0.5 and 27±1 µg/mL for TBARS and OxHLIA assays, respectively) and 
antimicrobial action against all bacteria and fungi strains evaluated (minimal inhibitory concentrations 
(MICs) ≤ 2.2 mg/mL). 
The results obtained allow to classify this tropical fruit epicarp as a potential source of bioactive 
compounds with great market value, since they can be applied in several industrial sectors, including 
the food and pharmaceutical industries.",
publisher = "Universidade da Madeira, Centro de Química da Madeira",
journal = "XV Encontro de Química dos Alimentos; 2021 Sep 5-8; Madeira, Portugal",
title = "By-products of tropical fruits as sources of bioactive molecules: Sicana odorifera (vell.) Naudin epicarp case study.",
pages = "81",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6574"
}

Albuquerque, B., Inês Dias, M., Pereira, C., Petrović, J., Soković, M., P.P. Oliveira, M.  Beatriz, C.F.C. Ferreira, I.,& Barros, L.. (2021). By-products of tropical fruits as sources of bioactive molecules: Sicana odorifera (vell.) Naudin epicarp case study.. in XV Encontro de Química dos Alimentos; 2021 Sep 5-8; Madeira, Portugal
Universidade da Madeira, Centro de Química da Madeira., 81.
https://hdl.handle.net/21.15107/rcub_ibiss_6574

Albuquerque B, Inês Dias M, Pereira C, Petrović J, Soković M, P.P. Oliveira, M.  Beatriz, C.F.C. Ferreira I, Barros L. By-products of tropical fruits as sources of bioactive molecules: Sicana odorifera (vell.) Naudin epicarp case study.. in XV Encontro de Química dos Alimentos; 2021 Sep 5-8; Madeira, Portugal. 2021;:81.
https://hdl.handle.net/21.15107/rcub_ibiss_6574 .

Albuquerque, Bianca, Inês Dias, Maria, Pereira, Carla, Petrović, Jovana, Soković, Marina, P.P. Oliveira, M.  Beatriz, C.F.C. Ferreira, Isabel, Barros, Lillian, "By-products of tropical fruits as sources of bioactive molecules: Sicana odorifera (vell.) Naudin epicarp case study." in XV Encontro de Química dos Alimentos; 2021 Sep 5-8; Madeira, Portugal (2021):81,
https://hdl.handle.net/21.15107/rcub_ibiss_6574 .