TY  - JOUR
AU  - Jeremić, Rada
AU  - Peković, Sanja
AU  - Lavrnja, Irena
AU  - Bjelobaba, Ivana
AU  - Đelić, Marina
AU  - Dacić, Sanja
AU  - Brkić, Predrag D
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5975
AB  - A growing body of evidence suggests that hyperbaric oxygenation (HBO) may affect the activity of adult neural stem cells (NSCs). Since the role of NSCs in recovery from brain injury is still unclear, the purpose of this study was to investigate the effects of sensorimotor cortex ablation (SCA) and HBO treatment (HBOT) on the processes of neurogenesis in the adult dentate gyrus (DG), a region of the hippocampus that is the site of adult neurogenesis. Ten-week-old Wistar rats were divided into groups: Control (C, intact animals), Sham control (S, animals that underwent the surgical procedure without opening the skull), SCA (animals in whom the right sensorimotor cortex was removed via suction ablation), and SCA + HBO (operated animals that passed HBOT). HBOT protocol: pressure applied at 2.5 absolute atmospheres for 60 min, once daily for 10 days. Using immunohistochemistry and double immunofluorescence labeling, we show that SCA causes significant loss of neurons in the DG. Newborn neurons in the subgranular zone (SGZ), inner-third, and partially mid-third of the granule cell layer are predominantly affected by SCA. HBOT decreases the SCA-caused loss of immature neurons, prevents reduction of dendritic arborization, and increases proliferation of progenitor cells. Our results suggest a protective effect of HBO by reducing the vulnerability of immature neurons in the adult DG to SCA injury.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Hyperbaric Oxygenation Prevents Loss of Immature Neurons in the Adult Hippocampal Dentate Gyrus Following Brain Injury
IS  - 5
VL  - 24
DO  - 10.3390/ijms24054261
SP  - 4261
ER  - 

@article{
author = "Jeremić, Rada and Peković, Sanja and Lavrnja, Irena and Bjelobaba, Ivana and Đelić, Marina and Dacić, Sanja and Brkić, Predrag D",
year = "2023",
abstract = "A growing body of evidence suggests that hyperbaric oxygenation (HBO) may affect the activity of adult neural stem cells (NSCs). Since the role of NSCs in recovery from brain injury is still unclear, the purpose of this study was to investigate the effects of sensorimotor cortex ablation (SCA) and HBO treatment (HBOT) on the processes of neurogenesis in the adult dentate gyrus (DG), a region of the hippocampus that is the site of adult neurogenesis. Ten-week-old Wistar rats were divided into groups: Control (C, intact animals), Sham control (S, animals that underwent the surgical procedure without opening the skull), SCA (animals in whom the right sensorimotor cortex was removed via suction ablation), and SCA + HBO (operated animals that passed HBOT). HBOT protocol: pressure applied at 2.5 absolute atmospheres for 60 min, once daily for 10 days. Using immunohistochemistry and double immunofluorescence labeling, we show that SCA causes significant loss of neurons in the DG. Newborn neurons in the subgranular zone (SGZ), inner-third, and partially mid-third of the granule cell layer are predominantly affected by SCA. HBOT decreases the SCA-caused loss of immature neurons, prevents reduction of dendritic arborization, and increases proliferation of progenitor cells. Our results suggest a protective effect of HBO by reducing the vulnerability of immature neurons in the adult DG to SCA injury.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Hyperbaric Oxygenation Prevents Loss of Immature Neurons in the Adult Hippocampal Dentate Gyrus Following Brain Injury",
number = "5",
volume = "24",
doi = "10.3390/ijms24054261",
pages = "4261"
}

Jeremić, R., Peković, S., Lavrnja, I., Bjelobaba, I., Đelić, M., Dacić, S.,& Brkić, P. D.. (2023). Hyperbaric Oxygenation Prevents Loss of Immature Neurons in the Adult Hippocampal Dentate Gyrus Following Brain Injury. in International Journal of Molecular Sciences
Basel: MDPI., 24(5), 4261.
https://doi.org/10.3390/ijms24054261

Jeremić R, Peković S, Lavrnja I, Bjelobaba I, Đelić M, Dacić S, Brkić PD. Hyperbaric Oxygenation Prevents Loss of Immature Neurons in the Adult Hippocampal Dentate Gyrus Following Brain Injury. in International Journal of Molecular Sciences. 2023;24(5):4261.
doi:10.3390/ijms24054261 .

Jeremić, Rada, Peković, Sanja, Lavrnja, Irena, Bjelobaba, Ivana, Đelić, Marina, Dacić, Sanja, Brkić, Predrag D, "Hyperbaric Oxygenation Prevents Loss of Immature Neurons in the Adult Hippocampal Dentate Gyrus Following Brain Injury" in International Journal of Molecular Sciences, 24, no. 5 (2023):4261,
https://doi.org/10.3390/ijms24054261 . .

TY  - CONF
AU  - Jeremić, Rada
AU  - Peković, Sanja
AU  - Lavrnja, Irena
AU  - Bjelobaba, Ivana
AU  - Đelić, Marina N
AU  - Brkić, Predrag D
AU  - Dacić, Sanja
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5984
AB  - Introduction: There is growing evidence that hyperbaric oxygenation (HBO) can affect adult neural stem cells (NSCs) activity. Because the role of NSCs in recovery from brain injury is still unclear, this study examined how ablation of the sensorimotor cortex (SCA) and HBO treatment (HBOT) affect the process of neurogenesis in the adult dentate gyrus (DG), a region of the hippocampus considered to be the site of adult neurogenesis. Material and methods: Ten-week-old Wistar rats were divided into groups: Control (C, intact animals), SCA (animals in which the right sensorimotor cortex was removed by suction ablation), and SCA+HBO (operated animals subjected to HBOT). HBOT protocol: pressure applied at 2.5 absolute atmospheres for 60 min, once daily for 10 days. The effects of HBOT were monitored by immunohistochemistry and double immunofluorescence labeling. In addition, the number of DCX+ cells was determined along the length of the SGZ in the inner and separately in the outer blade of the right dentate gyrus. Also, the total dendrite length was measured and the number of branching points, dendrite terminals, and segments were counted to quantify dendritic arborization in each neuron. Results: HBOT decreases SCA-induced loss of immature neurons, prevents reduction of dendritic branching, and increases proliferation of progenitor cells. Conclusion: Our results suggest a protective effect of HBOT by reducing the vulnerability of immature neurons in the adult DG to SCA injury.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Hyperbaric oxygen prevents dendrite degeneration and loss of DCX-positive newborn immature neurons in the dentate gyrus after traumatic brain injury
SP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5984
ER  - 

@conference{
author = "Jeremić, Rada and Peković, Sanja and Lavrnja, Irena and Bjelobaba, Ivana and Đelić, Marina N and Brkić, Predrag D and Dacić, Sanja",
year = "2023",
abstract = "Introduction: There is growing evidence that hyperbaric oxygenation (HBO) can affect adult neural stem cells (NSCs) activity. Because the role of NSCs in recovery from brain injury is still unclear, this study examined how ablation of the sensorimotor cortex (SCA) and HBO treatment (HBOT) affect the process of neurogenesis in the adult dentate gyrus (DG), a region of the hippocampus considered to be the site of adult neurogenesis. Material and methods: Ten-week-old Wistar rats were divided into groups: Control (C, intact animals), SCA (animals in which the right sensorimotor cortex was removed by suction ablation), and SCA+HBO (operated animals subjected to HBOT). HBOT protocol: pressure applied at 2.5 absolute atmospheres for 60 min, once daily for 10 days. The effects of HBOT were monitored by immunohistochemistry and double immunofluorescence labeling. In addition, the number of DCX+ cells was determined along the length of the SGZ in the inner and separately in the outer blade of the right dentate gyrus. Also, the total dendrite length was measured and the number of branching points, dendrite terminals, and segments were counted to quantify dendritic arborization in each neuron. Results: HBOT decreases SCA-induced loss of immature neurons, prevents reduction of dendritic branching, and increases proliferation of progenitor cells. Conclusion: Our results suggest a protective effect of HBOT by reducing the vulnerability of immature neurons in the adult DG to SCA injury.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Hyperbaric oxygen prevents dendrite degeneration and loss of DCX-positive newborn immature neurons in the dentate gyrus after traumatic brain injury",
pages = "78",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5984"
}

Jeremić, R., Peković, S., Lavrnja, I., Bjelobaba, I., Đelić, M. N., Brkić, P. D.,& Dacić, S.. (2023). Hyperbaric oxygen prevents dendrite degeneration and loss of DCX-positive newborn immature neurons in the dentate gyrus after traumatic brain injury. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 78.
https://hdl.handle.net/21.15107/rcub_ibiss_5984

Jeremić R, Peković S, Lavrnja I, Bjelobaba I, Đelić MN, Brkić PD, Dacić S. Hyperbaric oxygen prevents dendrite degeneration and loss of DCX-positive newborn immature neurons in the dentate gyrus after traumatic brain injury. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:78.
https://hdl.handle.net/21.15107/rcub_ibiss_5984 .

Jeremić, Rada, Peković, Sanja, Lavrnja, Irena, Bjelobaba, Ivana, Đelić, Marina N, Brkić, Predrag D, Dacić, Sanja, "Hyperbaric oxygen prevents dendrite degeneration and loss of DCX-positive newborn immature neurons in the dentate gyrus after traumatic brain injury" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):78,
https://hdl.handle.net/21.15107/rcub_ibiss_5984 .

TY  - JOUR
AU  - Pantić, Igor
AU  - Jeremić, Rada
AU  - Dacić, Sanja
AU  - Peković, Sanja
AU  - Pantić, Senka
AU  - Đelić, Marina
AU  - Vitić, Zagorka
AU  - Brkić, Predrag
AU  - Brodski, Claude
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3599
AB  - Traumatic brain injury (TBI) is a main cause of death and disabilities in young adults. Although learning and memory impairments are a major clinical manifestation of TBI, the consequences of TBI on the hippocampus are still not well understood. In particular, how lesions to the sensorimotor cortex damage the hippocampus, to which it is not directly connected, is still elusive. Here, we study the effects of sensorimotor cortex ablation (SCA) on the hippocampal dentate gyrus, by applying a highly sensitive gray-level co-occurrence matrix (GLCM) analysis. Using GLCM analysis of granule neurons, we discovered, in our TBI paradigm, subtle changes in granule cell (GC) morphology, including textual uniformity, contrast, and variance, which is not detected by conventional microscopy. We conclude that sensorimotor cortex trauma leads to specific changes in the hippocampus that advance our understanding of the cellular underpinnings of cognitive impairments in TBI. Moreover, we identified GLCM analysis as a highly sensitive method to detect subtle changes in the GC layers that is expected to significantly improve further studies investigating the impact of TBI on hippocampal neuropathology.
T2  - Microscopy and Microanalysis
T1  - Gray-Level Co-Occurrence Matrix Analysis of Granule Neurons of the Hippocampal Dentate Gyrus Following Cortical Injury.
IS  - 1
VL  - 26
DO  - 10.1017/S143192762000001X
SP  - 166
EP  - 172
ER  - 

@article{
author = "Pantić, Igor and Jeremić, Rada and Dacić, Sanja and Peković, Sanja and Pantić, Senka and Đelić, Marina and Vitić, Zagorka and Brkić, Predrag and Brodski, Claude",
year = "2020",
abstract = "Traumatic brain injury (TBI) is a main cause of death and disabilities in young adults. Although learning and memory impairments are a major clinical manifestation of TBI, the consequences of TBI on the hippocampus are still not well understood. In particular, how lesions to the sensorimotor cortex damage the hippocampus, to which it is not directly connected, is still elusive. Here, we study the effects of sensorimotor cortex ablation (SCA) on the hippocampal dentate gyrus, by applying a highly sensitive gray-level co-occurrence matrix (GLCM) analysis. Using GLCM analysis of granule neurons, we discovered, in our TBI paradigm, subtle changes in granule cell (GC) morphology, including textual uniformity, contrast, and variance, which is not detected by conventional microscopy. We conclude that sensorimotor cortex trauma leads to specific changes in the hippocampus that advance our understanding of the cellular underpinnings of cognitive impairments in TBI. Moreover, we identified GLCM analysis as a highly sensitive method to detect subtle changes in the GC layers that is expected to significantly improve further studies investigating the impact of TBI on hippocampal neuropathology.",
journal = "Microscopy and Microanalysis",
title = "Gray-Level Co-Occurrence Matrix Analysis of Granule Neurons of the Hippocampal Dentate Gyrus Following Cortical Injury.",
number = "1",
volume = "26",
doi = "10.1017/S143192762000001X",
pages = "166-172"
}

Pantić, I., Jeremić, R., Dacić, S., Peković, S., Pantić, S., Đelić, M., Vitić, Z., Brkić, P.,& Brodski, C.. (2020). Gray-Level Co-Occurrence Matrix Analysis of Granule Neurons of the Hippocampal Dentate Gyrus Following Cortical Injury.. in Microscopy and Microanalysis, 26(1), 166-172.
https://doi.org/10.1017/S143192762000001X

Pantić I, Jeremić R, Dacić S, Peković S, Pantić S, Đelić M, Vitić Z, Brkić P, Brodski C. Gray-Level Co-Occurrence Matrix Analysis of Granule Neurons of the Hippocampal Dentate Gyrus Following Cortical Injury.. in Microscopy and Microanalysis. 2020;26(1):166-172.
doi:10.1017/S143192762000001X .

Pantić, Igor, Jeremić, Rada, Dacić, Sanja, Peković, Sanja, Pantić, Senka, Đelić, Marina, Vitić, Zagorka, Brkić, Predrag, Brodski, Claude, "Gray-Level Co-Occurrence Matrix Analysis of Granule Neurons of the Hippocampal Dentate Gyrus Following Cortical Injury." in Microscopy and Microanalysis, 26, no. 1 (2020):166-172,
https://doi.org/10.1017/S143192762000001X . .

TY  - CONF
AU  - Brkić, Predrag D
AU  - Jovanović, Tomislav S
AU  - Krstić, Danijela Z
AU  - Stojiljković, Mirjana B
AU  - Colović, Mirjana B
AU  - Dačić, Sanja
AU  - Mitrović, Ana
AU  - Bjelobaba, Ivana
AU  - Savić, Danijela
AU  - Parbucki, Ana
AU  - Lavrnja, Irena
AU  - Rakić, Ljubisav
AU  - Peković, Sanja
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1188
C3  - Brain Injury
T1  - Hyperbaric oxygenation reduces neuronal degeneration by regulation of oxidant/antioxidant status in the rat brain tissue after cortical injury
IS  - 4-5
VL  - 26
EP  - 487
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1188
ER  - 

@conference{
author = "Brkić, Predrag D and Jovanović, Tomislav S and Krstić, Danijela Z and Stojiljković, Mirjana B and Colović, Mirjana B and Dačić, Sanja and Mitrović, Ana and Bjelobaba, Ivana and Savić, Danijela and Parbucki, Ana and Lavrnja, Irena and Rakić, Ljubisav and Peković, Sanja",
year = "2012",
journal = "Brain Injury",
title = "Hyperbaric oxygenation reduces neuronal degeneration by regulation of oxidant/antioxidant status in the rat brain tissue after cortical injury",
number = "4-5",
volume = "26",
pages = "487",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1188"
}

Brkić, P. D., Jovanović, T. S., Krstić, D. Z., Stojiljković, M. B., Colović, M. B., Dačić, S., Mitrović, A., Bjelobaba, I., Savić, D., Parbucki, A., Lavrnja, I., Rakić, L.,& Peković, S.. (2012). Hyperbaric oxygenation reduces neuronal degeneration by regulation of oxidant/antioxidant status in the rat brain tissue after cortical injury. in Brain Injury, 26(4-5).
https://hdl.handle.net/21.15107/rcub_ibiss_1188

Brkić PD, Jovanović TS, Krstić DZ, Stojiljković MB, Colović MB, Dačić S, Mitrović A, Bjelobaba I, Savić D, Parbucki A, Lavrnja I, Rakić L, Peković S. Hyperbaric oxygenation reduces neuronal degeneration by regulation of oxidant/antioxidant status in the rat brain tissue after cortical injury. in Brain Injury. 2012;26(4-5):null-487.
https://hdl.handle.net/21.15107/rcub_ibiss_1188 .

Brkić, Predrag D, Jovanović, Tomislav S, Krstić, Danijela Z, Stojiljković, Mirjana B, Colović, Mirjana B, Dačić, Sanja, Mitrović, Ana, Bjelobaba, Ivana, Savić, Danijela, Parbucki, Ana, Lavrnja, Irena, Rakić, Ljubisav, Peković, Sanja, "Hyperbaric oxygenation reduces neuronal degeneration by regulation of oxidant/antioxidant status in the rat brain tissue after cortical injury" in Brain Injury, 26, no. 4-5 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1188 .

TY  - JOUR
AU  - Brkić, Predrag D
AU  - Stojiljković, Mirjana B
AU  - Jovanović, Tomislav S
AU  - Dačić, Sanja
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Parabučki, Ana B.
AU  - Bjelobaba, Ivana
AU  - Rakić, Ljubisav
AU  - Peković, Sanja
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1146
AB  - Objective: To investigate whether hyperbaric oxygenation (HBO) can improve the recovery of motor functions in rats after suction ablation of the right sensorimotor cortex. Methods: The experimental paradigm implies the following groups: Control animals (C), Control+HBO (CHBO), Sham controls (S), Sham control+HBO (SHBO), Lesion group (L), right sensorimotor cortex was removed by suction, Lesion+HBO (LHBO). Hyperbaric protocol: pressure applied 2.5 atmospheres absolute, for 60 minutes, once a day for 10 days. A beam walking test and grip strength meter were used to evaluate the recovery of motor functions. Expression profiles of growth-associated protein 43 (GAP43) and synaptophysin (SYP) were detected using immunohistochemistry. Results: The LHBO group achieved statistically superior scores in the beam walking test compared to the L group. Additionally, the recovery of muscle strength of the affected hindpaw was significantly enhanced after HBO treatment. Hyperbaric oxygenation induced over-expression of GAP43 and SYP in the neurons surrounding the lesion site. Conclusions: Data presented suggest that hyperbaric oxygen therapy can intensify neuroplastic responses by promoting axonal sprouting and synapse remodelling, which contributes to the recovery of locomotor performances in rats. This provides the perspective for implementation of HBO in clinical strategies for treating traumatic brain injuries.
T2  - Brain Injury
T1  - Hyperbaric oxygenation improves locomotor ability by enhancing neuroplastic responses after cortical ablation in rats
IS  - 10
VL  - 26
SP  - 961
EP  - 1284
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1146
ER  - 

@article{
author = "Brkić, Predrag D and Stojiljković, Mirjana B and Jovanović, Tomislav S and Dačić, Sanja and Lavrnja, Irena and Savić, Danijela and Parabučki, Ana B. and Bjelobaba, Ivana and Rakić, Ljubisav and Peković, Sanja",
year = "2012",
abstract = "Objective: To investigate whether hyperbaric oxygenation (HBO) can improve the recovery of motor functions in rats after suction ablation of the right sensorimotor cortex. Methods: The experimental paradigm implies the following groups: Control animals (C), Control+HBO (CHBO), Sham controls (S), Sham control+HBO (SHBO), Lesion group (L), right sensorimotor cortex was removed by suction, Lesion+HBO (LHBO). Hyperbaric protocol: pressure applied 2.5 atmospheres absolute, for 60 minutes, once a day for 10 days. A beam walking test and grip strength meter were used to evaluate the recovery of motor functions. Expression profiles of growth-associated protein 43 (GAP43) and synaptophysin (SYP) were detected using immunohistochemistry. Results: The LHBO group achieved statistically superior scores in the beam walking test compared to the L group. Additionally, the recovery of muscle strength of the affected hindpaw was significantly enhanced after HBO treatment. Hyperbaric oxygenation induced over-expression of GAP43 and SYP in the neurons surrounding the lesion site. Conclusions: Data presented suggest that hyperbaric oxygen therapy can intensify neuroplastic responses by promoting axonal sprouting and synapse remodelling, which contributes to the recovery of locomotor performances in rats. This provides the perspective for implementation of HBO in clinical strategies for treating traumatic brain injuries.",
journal = "Brain Injury",
title = "Hyperbaric oxygenation improves locomotor ability by enhancing neuroplastic responses after cortical ablation in rats",
number = "10",
volume = "26",
pages = "961-1284",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1146"
}

Brkić, P. D., Stojiljković, M. B., Jovanović, T. S., Dačić, S., Lavrnja, I., Savić, D., Parabučki, A. B., Bjelobaba, I., Rakić, L.,& Peković, S.. (2012). Hyperbaric oxygenation improves locomotor ability by enhancing neuroplastic responses after cortical ablation in rats. in Brain Injury, 26(10), 961-1284.
https://hdl.handle.net/21.15107/rcub_ibiss_1146

Brkić PD, Stojiljković MB, Jovanović TS, Dačić S, Lavrnja I, Savić D, Parabučki AB, Bjelobaba I, Rakić L, Peković S. Hyperbaric oxygenation improves locomotor ability by enhancing neuroplastic responses after cortical ablation in rats. in Brain Injury. 2012;26(10):961-1284.
https://hdl.handle.net/21.15107/rcub_ibiss_1146 .

Brkić, Predrag D, Stojiljković, Mirjana B, Jovanović, Tomislav S, Dačić, Sanja, Lavrnja, Irena, Savić, Danijela, Parabučki, Ana B., Bjelobaba, Ivana, Rakić, Ljubisav, Peković, Sanja, "Hyperbaric oxygenation improves locomotor ability by enhancing neuroplastic responses after cortical ablation in rats" in Brain Injury, 26, no. 10 (2012):961-1284,
https://hdl.handle.net/21.15107/rcub_ibiss_1146 .

TY  - JOUR
AU  - Parabučki, Ana B.
AU  - Božić, Iva D.
AU  - Bjelobaba, Ivana
AU  - Lavrnja, Irena
AU  - Brkić, Predrag D
AU  - Jovanović, Tomislav S
AU  - Savić, Danijela
AU  - Stojiljković, Mirjana B
AU  - Peković, Sanja
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1072
AB  - Aim To evaluate the effect of hyperbaric oxygen therapy (HBOT) on superoxide dismutase 2 (SOD2) expression pattern after the cortical stab injury (CSI). Methods CSI was performed on 88 male Wistar rats, divided into control, sham, lesioned, and HBO groups. HBOT protocol was the following: pressure applied was 2.5 absolute atmospheres, for 60 minutes, once a day for consecutive 3 or 10 days. The pattern of SOD2 expression and cellular localization was analyzed using real-time polymerase chain reaction, Western blot, and double-label fluorescence immunohistochemistry. Neurons undergoing degeneration were visualized with Fluoro-Jade (R) B. Results CSI induced significant transient increase in SOD2 protein levels at day 3 post injury, which was followed by a reduction toward control levels at post-injury day 10. At the same time points, mRNA levels for SOD2 in the injured cortex were down-regulated. Exposure to HBO for 3 days considerably down-regulated SOD2 protein levels in the injured cortex, while after 10 days of HBOT an up-regulation of SOD2 was observed. HBOT significantly increased mRNA levels for SOD2 at both time points compared to the corresponding L group, but they were still lower than in controls. Double immunofluorescence staining revealed that 3 days after CSI, up-regulation of SOD2 was mostly due to an increased expression in reactive astrocytes surrounding the lesion site. HBOT attenuated SOD2 expression both in neuronal and astroglial cells. Fluoro-Jade (R) B labeling showed that HBOT significantly decreased the number of degenerating neurons in the injured cortex. Conclusion HBOT alters SOD2 protein and mRNA levels after brain injury in a time-dependent manner.
T2  - Croatian Medical Journal
T1  - Hyperbaric oxygenation alters temporal expression pattern of superoxide dismutase 2 after cortical stab injury in rats
IS  - 6
VL  - 53
SP  - 217
EP  - 597
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1072
ER  - 

@article{
author = "Parabučki, Ana B. and Božić, Iva D. and Bjelobaba, Ivana and Lavrnja, Irena and Brkić, Predrag D and Jovanović, Tomislav S and Savić, Danijela and Stojiljković, Mirjana B and Peković, Sanja",
year = "2012",
abstract = "Aim To evaluate the effect of hyperbaric oxygen therapy (HBOT) on superoxide dismutase 2 (SOD2) expression pattern after the cortical stab injury (CSI). Methods CSI was performed on 88 male Wistar rats, divided into control, sham, lesioned, and HBO groups. HBOT protocol was the following: pressure applied was 2.5 absolute atmospheres, for 60 minutes, once a day for consecutive 3 or 10 days. The pattern of SOD2 expression and cellular localization was analyzed using real-time polymerase chain reaction, Western blot, and double-label fluorescence immunohistochemistry. Neurons undergoing degeneration were visualized with Fluoro-Jade (R) B. Results CSI induced significant transient increase in SOD2 protein levels at day 3 post injury, which was followed by a reduction toward control levels at post-injury day 10. At the same time points, mRNA levels for SOD2 in the injured cortex were down-regulated. Exposure to HBO for 3 days considerably down-regulated SOD2 protein levels in the injured cortex, while after 10 days of HBOT an up-regulation of SOD2 was observed. HBOT significantly increased mRNA levels for SOD2 at both time points compared to the corresponding L group, but they were still lower than in controls. Double immunofluorescence staining revealed that 3 days after CSI, up-regulation of SOD2 was mostly due to an increased expression in reactive astrocytes surrounding the lesion site. HBOT attenuated SOD2 expression both in neuronal and astroglial cells. Fluoro-Jade (R) B labeling showed that HBOT significantly decreased the number of degenerating neurons in the injured cortex. Conclusion HBOT alters SOD2 protein and mRNA levels after brain injury in a time-dependent manner.",
journal = "Croatian Medical Journal",
title = "Hyperbaric oxygenation alters temporal expression pattern of superoxide dismutase 2 after cortical stab injury in rats",
number = "6",
volume = "53",
pages = "217-597",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1072"
}

Parabučki, A. B., Božić, I. D., Bjelobaba, I., Lavrnja, I., Brkić, P. D., Jovanović, T. S., Savić, D., Stojiljković, M. B.,& Peković, S.. (2012). Hyperbaric oxygenation alters temporal expression pattern of superoxide dismutase 2 after cortical stab injury in rats. in Croatian Medical Journal, 53(6), 217-597.
https://hdl.handle.net/21.15107/rcub_ibiss_1072

Parabučki AB, Božić ID, Bjelobaba I, Lavrnja I, Brkić PD, Jovanović TS, Savić D, Stojiljković MB, Peković S. Hyperbaric oxygenation alters temporal expression pattern of superoxide dismutase 2 after cortical stab injury in rats. in Croatian Medical Journal. 2012;53(6):217-597.
https://hdl.handle.net/21.15107/rcub_ibiss_1072 .

Parabučki, Ana B., Božić, Iva D., Bjelobaba, Ivana, Lavrnja, Irena, Brkić, Predrag D, Jovanović, Tomislav S, Savić, Danijela, Stojiljković, Mirjana B, Peković, Sanja, "Hyperbaric oxygenation alters temporal expression pattern of superoxide dismutase 2 after cortical stab injury in rats" in Croatian Medical Journal, 53, no. 6 (2012):217-597,
https://hdl.handle.net/21.15107/rcub_ibiss_1072 .