TY  - JOUR
AU  - Vesović, Nikola
AU  - Nenadić, Marija
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Vujisić, Ljubodrag V
AU  - Todosijević, Marina M.
AU  - Stevanović, Nataša
AU  - Perić Mataruga, Vesna
AU  - Ilijin, Larisa
AU  - Ćurčić, Srećko
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4911
AB  - Pygidial gland secretions are used as repellent defensive allomones in ground beetles. We provide the first precise data on the chemical composition and antimicrobial potency of the secretion of the blue ground beetle, as well as on the morphology of its pygidial glands. The latter structures were not previously studied chemoecologically and morphologically, and we hypothesized that their secretion may have some antimicrobial action, as is the case with certain Carabus species. Gas chromatography-mass spectrometry (GC-MS) was used to identify methacrylic and angelic acids as dominant chemicals in the secretion from individuals of three populations of the blue ground beetle in Serbia. We tested its secretion against selected strains of medically important microorganisms. The secretion exibits antimicrobial action against certain bacterial species and all tested micromycetes. The most significant antifungal effect of the secretion was against Penicillium ochrochloron, which is more sensitive to the secretion than to commercial antifungal drugs ketoconazole and bifonazole. Bifonazole achieved minimum inhibitory concentrations against Trichoderma viride at more than three times higher value than did the secretion, indicating a significant antifungal effect of the secretion against this micromycete as well. Additionally, we tested commercially available standards of two dominant chemicals in the secretion to investigate their interaction and antimicrobial role in the secretion. Finally, we describe all glandular morpho-functional units of the blue ground beetle. Our results suggest that the secretion of the blue ground beetle may serve not only defensive but also antimicrobial functions, which likely aid the survival of this beetle in the microbial-rich forest litter habitat.
PB  - Springer-Verlag GmbH Germany
T2  - The Science of Nature
T1  - Pygidial glands of the blue ground beetle Carabus intricatus: chemical composition of the secretion and its antimicrobial activity
IS  - 2
VL  - 109
DO  - 10.1007/s00114-022-01790-0
SP  - 19
ER  - 

@article{
author = "Vesović, Nikola and Nenadić, Marija and Soković, Marina and Ćirić, Ana and Vujisić, Ljubodrag V and Todosijević, Marina M. and Stevanović, Nataša and Perić Mataruga, Vesna and Ilijin, Larisa and Ćurčić, Srećko",
year = "2022",
abstract = "Pygidial gland secretions are used as repellent defensive allomones in ground beetles. We provide the first precise data on the chemical composition and antimicrobial potency of the secretion of the blue ground beetle, as well as on the morphology of its pygidial glands. The latter structures were not previously studied chemoecologically and morphologically, and we hypothesized that their secretion may have some antimicrobial action, as is the case with certain Carabus species. Gas chromatography-mass spectrometry (GC-MS) was used to identify methacrylic and angelic acids as dominant chemicals in the secretion from individuals of three populations of the blue ground beetle in Serbia. We tested its secretion against selected strains of medically important microorganisms. The secretion exibits antimicrobial action against certain bacterial species and all tested micromycetes. The most significant antifungal effect of the secretion was against Penicillium ochrochloron, which is more sensitive to the secretion than to commercial antifungal drugs ketoconazole and bifonazole. Bifonazole achieved minimum inhibitory concentrations against Trichoderma viride at more than three times higher value than did the secretion, indicating a significant antifungal effect of the secretion against this micromycete as well. Additionally, we tested commercially available standards of two dominant chemicals in the secretion to investigate their interaction and antimicrobial role in the secretion. Finally, we describe all glandular morpho-functional units of the blue ground beetle. Our results suggest that the secretion of the blue ground beetle may serve not only defensive but also antimicrobial functions, which likely aid the survival of this beetle in the microbial-rich forest litter habitat.",
publisher = "Springer-Verlag GmbH Germany",
journal = "The Science of Nature",
title = "Pygidial glands of the blue ground beetle Carabus intricatus: chemical composition of the secretion and its antimicrobial activity",
number = "2",
volume = "109",
doi = "10.1007/s00114-022-01790-0",
pages = "19"
}

Vesović, N., Nenadić, M., Soković, M., Ćirić, A., Vujisić, L. V., Todosijević, M. M., Stevanović, N., Perić Mataruga, V., Ilijin, L.,& Ćurčić, S.. (2022). Pygidial glands of the blue ground beetle Carabus intricatus: chemical composition of the secretion and its antimicrobial activity. in The Science of Nature
Springer-Verlag GmbH Germany., 109(2), 19.
https://doi.org/10.1007/s00114-022-01790-0

Vesović N, Nenadić M, Soković M, Ćirić A, Vujisić LV, Todosijević MM, Stevanović N, Perić Mataruga V, Ilijin L, Ćurčić S. Pygidial glands of the blue ground beetle Carabus intricatus: chemical composition of the secretion and its antimicrobial activity. in The Science of Nature. 2022;109(2):19.
doi:10.1007/s00114-022-01790-0 .

Vesović, Nikola, Nenadić, Marija, Soković, Marina, Ćirić, Ana, Vujisić, Ljubodrag V, Todosijević, Marina M., Stevanović, Nataša, Perić Mataruga, Vesna, Ilijin, Larisa, Ćurčić, Srećko, "Pygidial glands of the blue ground beetle Carabus intricatus: chemical composition of the secretion and its antimicrobial activity" in The Science of Nature, 109, no. 2 (2022):19,
https://doi.org/10.1007/s00114-022-01790-0 . .

TY  - JOUR
AU  - Nenadić, Marija
AU  - Soković, Marina
AU  - Calhelha, Ricardo C.
AU  - Ferreira, Isabel C. F. R.
AU  - Ćirić, Ana
AU  - Vesović, Nikola
AU  - Ćurčić, Srećko
PY  - 2018
UR  - http://link.springer.com/10.2478/s11756-018-0082-x
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3092
AB  - Inhibition of the proliferation of human tumour cells and porcine non-tumour cells by the pygidial gland secretion released by adults of four ground beetle species was observed in this study. The sulphorhodamine B (SRB) assay was applied to establish the percentages of inhibition of the net growth of four human tumour cell lines and porcine liver primary non-tumour cells. The secretions of all tested ground beetle species were shown to have an antiproliferative effect on the tested cell lines. Special emphasis is put on the secretion of Abax parallelepipedus, which showed the highest antitumour potential and weakest inhibition of non-tumour cell proliferation. The antitumour and antiproliferative potential of the pygidial gland secretions of ground beetles is here demonstrated for the first time. It is suggested that certain organic acids are responsible for the action. Further investigation needs to be conducted in order to better understand the mechanisms governing the observed cytotoxic and antitumour activity.
T2  - Biologia
T1  - Inhibition of tumour and non-tumour cell proliferation by pygidial gland secretions of four ground beetle species (Coleoptera: Carabidae)
DO  - 10.2478/s11756-018-0082-x
ER  - 

@article{
author = "Nenadić, Marija and Soković, Marina and Calhelha, Ricardo C. and Ferreira, Isabel C. F. R. and Ćirić, Ana and Vesović, Nikola and Ćurčić, Srećko",
year = "2018",
abstract = "Inhibition of the proliferation of human tumour cells and porcine non-tumour cells by the pygidial gland secretion released by adults of four ground beetle species was observed in this study. The sulphorhodamine B (SRB) assay was applied to establish the percentages of inhibition of the net growth of four human tumour cell lines and porcine liver primary non-tumour cells. The secretions of all tested ground beetle species were shown to have an antiproliferative effect on the tested cell lines. Special emphasis is put on the secretion of Abax parallelepipedus, which showed the highest antitumour potential and weakest inhibition of non-tumour cell proliferation. The antitumour and antiproliferative potential of the pygidial gland secretions of ground beetles is here demonstrated for the first time. It is suggested that certain organic acids are responsible for the action. Further investigation needs to be conducted in order to better understand the mechanisms governing the observed cytotoxic and antitumour activity.",
journal = "Biologia",
title = "Inhibition of tumour and non-tumour cell proliferation by pygidial gland secretions of four ground beetle species (Coleoptera: Carabidae)",
doi = "10.2478/s11756-018-0082-x"
}

Nenadić, M., Soković, M., Calhelha, R. C., Ferreira, I. C. F. R., Ćirić, A., Vesović, N.,& Ćurčić, S.. (2018). Inhibition of tumour and non-tumour cell proliferation by pygidial gland secretions of four ground beetle species (Coleoptera: Carabidae). in Biologia.
https://doi.org/10.2478/s11756-018-0082-x

Nenadić M, Soković M, Calhelha RC, Ferreira ICFR, Ćirić A, Vesović N, Ćurčić S. Inhibition of tumour and non-tumour cell proliferation by pygidial gland secretions of four ground beetle species (Coleoptera: Carabidae). in Biologia. 2018;.
doi:10.2478/s11756-018-0082-x .

Nenadić, Marija, Soković, Marina, Calhelha, Ricardo C., Ferreira, Isabel C. F. R., Ćirić, Ana, Vesović, Nikola, Ćurčić, Srećko, "Inhibition of tumour and non-tumour cell proliferation by pygidial gland secretions of four ground beetle species (Coleoptera: Carabidae)" in Biologia (2018),
https://doi.org/10.2478/s11756-018-0082-x . .

TY  - JOUR
AU  - Nenadić, Marija
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Perić Mataruga, Vesna
AU  - Ilijin, Larisa
AU  - Tešević, Vele
AU  - Todosijević, Marina
AU  - Vujisić, Ljubodrag
AU  - Vesović, Nikola
AU  - Ćurčić, Srećko
PY  - 2017
UR  - http://link.springer.com/10.1007/s00253-016-8082-7
UR  - https://www.scopus.com/record/display.uri?eid=2-s2.0-85008708376&origin=SingleRecordEmailAlert&dgcid=scalert_sc_search_email&txGid=BCBFF82A73D51FA0ED62BC41FE5E5987.wsnAw8kcdt7IPYLO0V48gA%3A37
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2511
AB  - Recently, various insect secretions have been tested as possible antimicrobial agents. In beetles, these secretions are essentially products of various exocrine glands, of which particular emphasis is on pygidial glands that are common for the suborder Adephaga. The antimicrobial potential of the pygidial gland secretion isolated from the adults of Calosoma sycophanta against human pathogens has been tested and compared with the potential of other carabid species, particularly within the tribe Carabini. The antimicrobial assay includes a microdilution method which was applied in order to determine the minimal inhibitory, minimal fungicidal and minimal bactericidal concentrations. It has been tested the effect of the secretion against eight strains of fungal and eight strains of bacterial species. The secretion sample of the tested carabid species has shown statistically significant antifungal effect against all strains of treated micromycetes, the highest in comparison with previously tested carabids (Carabus spp., Laemostenus punctatus and Abax parallelepipedus). Aspergillus versicolor proved to be the most sensitive micromycete, while the remaining seven fungal strains have shown the same level of sensibility. In comparison with commercial mycotics ketoconazole and bifonazole, applied as positive controls, the tested secretion showed much higher antifungal activity for all fungal strains. Antibacterial effect has been manifested only against one bacterial strain (Escherichia coli), contrary to other previously studied carabid species. These observations might have a significant impact on the ecological domain and possible purpose in biomedical studies and applications in the future. Additionally, morpho-histology of the pygidial glands of C. sycophanta is investigated and discussed.
T2  - Applied Microbiology and Biotechnology
T1  - The pygidial gland secretion of the forest caterpillar hunter, Calosoma (Calosoma) sycophanta: the antimicrobial properties against human pathogens
IS  - 3
VL  - 101
DO  - 10.1007/s00253-016-8082-7
SP  - 977
EP  - 985
ER  - 

@article{
author = "Nenadić, Marija and Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana and Perić Mataruga, Vesna and Ilijin, Larisa and Tešević, Vele and Todosijević, Marina and Vujisić, Ljubodrag and Vesović, Nikola and Ćurčić, Srećko",
year = "2017",
abstract = "Recently, various insect secretions have been tested as possible antimicrobial agents. In beetles, these secretions are essentially products of various exocrine glands, of which particular emphasis is on pygidial glands that are common for the suborder Adephaga. The antimicrobial potential of the pygidial gland secretion isolated from the adults of Calosoma sycophanta against human pathogens has been tested and compared with the potential of other carabid species, particularly within the tribe Carabini. The antimicrobial assay includes a microdilution method which was applied in order to determine the minimal inhibitory, minimal fungicidal and minimal bactericidal concentrations. It has been tested the effect of the secretion against eight strains of fungal and eight strains of bacterial species. The secretion sample of the tested carabid species has shown statistically significant antifungal effect against all strains of treated micromycetes, the highest in comparison with previously tested carabids (Carabus spp., Laemostenus punctatus and Abax parallelepipedus). Aspergillus versicolor proved to be the most sensitive micromycete, while the remaining seven fungal strains have shown the same level of sensibility. In comparison with commercial mycotics ketoconazole and bifonazole, applied as positive controls, the tested secretion showed much higher antifungal activity for all fungal strains. Antibacterial effect has been manifested only against one bacterial strain (Escherichia coli), contrary to other previously studied carabid species. These observations might have a significant impact on the ecological domain and possible purpose in biomedical studies and applications in the future. Additionally, morpho-histology of the pygidial glands of C. sycophanta is investigated and discussed.",
journal = "Applied Microbiology and Biotechnology",
title = "The pygidial gland secretion of the forest caterpillar hunter, Calosoma (Calosoma) sycophanta: the antimicrobial properties against human pathogens",
number = "3",
volume = "101",
doi = "10.1007/s00253-016-8082-7",
pages = "977-985"
}

Nenadić, M., Soković, M., Glamočlija, J., Ćirić, A., Perić Mataruga, V., Ilijin, L., Tešević, V., Todosijević, M., Vujisić, L., Vesović, N.,& Ćurčić, S.. (2017). The pygidial gland secretion of the forest caterpillar hunter, Calosoma (Calosoma) sycophanta: the antimicrobial properties against human pathogens. in Applied Microbiology and Biotechnology, 101(3), 977-985.
https://doi.org/10.1007/s00253-016-8082-7

Nenadić M, Soković M, Glamočlija J, Ćirić A, Perić Mataruga V, Ilijin L, Tešević V, Todosijević M, Vujisić L, Vesović N, Ćurčić S. The pygidial gland secretion of the forest caterpillar hunter, Calosoma (Calosoma) sycophanta: the antimicrobial properties against human pathogens. in Applied Microbiology and Biotechnology. 2017;101(3):977-985.
doi:10.1007/s00253-016-8082-7 .

Nenadić, Marija, Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, Perić Mataruga, Vesna, Ilijin, Larisa, Tešević, Vele, Todosijević, Marina, Vujisić, Ljubodrag, Vesović, Nikola, Ćurčić, Srećko, "The pygidial gland secretion of the forest caterpillar hunter, Calosoma (Calosoma) sycophanta: the antimicrobial properties against human pathogens" in Applied Microbiology and Biotechnology, 101, no. 3 (2017):977-985,
https://doi.org/10.1007/s00253-016-8082-7 . .

TY  - JOUR
AU  - Vesović, Nikola
AU  - Vujisić, Ljubodrag
AU  - Perić Mataruga, Vesna
AU  - Krstić, Gordana
AU  - Nenadić, Marija
AU  - Cvetković, Mirjana
AU  - Ilijin, Larisa
AU  - Stanković, Jovana
AU  - Ćurčić, Srećko
PY  - 2017
UR  - https://www.tandfonline.com/doi/full/10.1080/00222933.2017.1293183
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2682
AB  - Although all ground beetles have pygidial glands homologous in structure and function, there are many differences among species, often significant, in terms of chemical composition of the secretion and morphology of the gland components. In this paper, two predatory ground beetle species of the genus Carabus, namely C. (Tomocarabus) convexus and C. (Procrustes) coriaceus, were subjected to chemical, morphological and histological study of their pygidial glands and the glands’ secretions. Altogether, three carboxylic acids were isolated, and the pygidial gland reservoir and other glandular parts are morphologically described in the study. We also tested whether there exist differences in chemical content of the secretion obtained by upsetting the beetles in a traditional way and that obtained directly from intact reservoirs. Detailed data on morphology of the pygidial glands of both species are presented, as well as updated information about the chemical components of the glandular secretions. Apart from tiglic and methacrylic acids, which are typical for Carabus pygidial secretions, we also found benzoic acid as a minor component of the secretion in both species. In addition, a chemotaxonomic overview of Carabus taxa is given in the paper.
T2  - Journal of Natural History
T1  - Chemical secretion and morpho-histology of the pygidial glands in two Palaearctic predatory ground beetle species: Carabus ( Tomocarabus ) convexus and C . ( Procrustes ) coriaceus (Coleoptera: Carabidae)
IS  - 9-10
VL  - 51
DO  - 10.1080/00222933.2017.1293183
SP  - 545
EP  - 560
ER  - 

@article{
author = "Vesović, Nikola and Vujisić, Ljubodrag and Perić Mataruga, Vesna and Krstić, Gordana and Nenadić, Marija and Cvetković, Mirjana and Ilijin, Larisa and Stanković, Jovana and Ćurčić, Srećko",
year = "2017",
abstract = "Although all ground beetles have pygidial glands homologous in structure and function, there are many differences among species, often significant, in terms of chemical composition of the secretion and morphology of the gland components. In this paper, two predatory ground beetle species of the genus Carabus, namely C. (Tomocarabus) convexus and C. (Procrustes) coriaceus, were subjected to chemical, morphological and histological study of their pygidial glands and the glands’ secretions. Altogether, three carboxylic acids were isolated, and the pygidial gland reservoir and other glandular parts are morphologically described in the study. We also tested whether there exist differences in chemical content of the secretion obtained by upsetting the beetles in a traditional way and that obtained directly from intact reservoirs. Detailed data on morphology of the pygidial glands of both species are presented, as well as updated information about the chemical components of the glandular secretions. Apart from tiglic and methacrylic acids, which are typical for Carabus pygidial secretions, we also found benzoic acid as a minor component of the secretion in both species. In addition, a chemotaxonomic overview of Carabus taxa is given in the paper.",
journal = "Journal of Natural History",
title = "Chemical secretion and morpho-histology of the pygidial glands in two Palaearctic predatory ground beetle species: Carabus ( Tomocarabus ) convexus and C . ( Procrustes ) coriaceus (Coleoptera: Carabidae)",
number = "9-10",
volume = "51",
doi = "10.1080/00222933.2017.1293183",
pages = "545-560"
}

Vesović, N., Vujisić, L., Perić Mataruga, V., Krstić, G., Nenadić, M., Cvetković, M., Ilijin, L., Stanković, J.,& Ćurčić, S.. (2017). Chemical secretion and morpho-histology of the pygidial glands in two Palaearctic predatory ground beetle species: Carabus ( Tomocarabus ) convexus and C . ( Procrustes ) coriaceus (Coleoptera: Carabidae). in Journal of Natural History, 51(9-10), 545-560.
https://doi.org/10.1080/00222933.2017.1293183

Vesović N, Vujisić L, Perić Mataruga V, Krstić G, Nenadić M, Cvetković M, Ilijin L, Stanković J, Ćurčić S. Chemical secretion and morpho-histology of the pygidial glands in two Palaearctic predatory ground beetle species: Carabus ( Tomocarabus ) convexus and C . ( Procrustes ) coriaceus (Coleoptera: Carabidae). in Journal of Natural History. 2017;51(9-10):545-560.
doi:10.1080/00222933.2017.1293183 .

Vesović, Nikola, Vujisić, Ljubodrag, Perić Mataruga, Vesna, Krstić, Gordana, Nenadić, Marija, Cvetković, Mirjana, Ilijin, Larisa, Stanković, Jovana, Ćurčić, Srećko, "Chemical secretion and morpho-histology of the pygidial glands in two Palaearctic predatory ground beetle species: Carabus ( Tomocarabus ) convexus and C . ( Procrustes ) coriaceus (Coleoptera: Carabidae)" in Journal of Natural History, 51, no. 9-10 (2017):545-560,
https://doi.org/10.1080/00222933.2017.1293183 . .

TY  - JOUR
AU  - Nenadić, Marija
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Perić Mataruga, Vesna
AU  - Ilijin, Larisa
AU  - Tešević, Vele
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Vesović, Nikola
AU  - Ćurčić, Srećko
PY  - 2016
UR  - https://link.springer.com/article/10.1007/s00114-016-1358-z
UR  - https://pubmed.ncbi.nlm.nih.gov/27000261/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3981
AB  - The antimicrobial properties of the pygidial gland secretions released by the adults of the three ground beetle species, Carabus ullrichii, C. coriaceus, and Abax parallelepipedus, have been tested. Microdilution method was applied for detection of minimal inhibitory concentrations (MICs), minimal bactericidal concentrations (MBCs), and minimal fungicidal concentrations (MFCs). Additionally, morpho-histology of the pygidial glands is investigated. We have tested 16 laboratory and clinical strains of human pathogens-eight bacterial both gram-positive and gram-negative species and eight fungal species. The pygidial secretion samples of C. ullrichii have showed the strongest antimicrobial effect against all strains of treated bacteria and fungi. Staphylococcus aureus, Lysteria monocytogenes, and Salmonella typhimurium proved to be the most sensitive bacterial strains. Penicillium funiculosum proved to be the most sensitive micromycete, while P. ochrochloron and P. verrucosum var. cyclopium the most resistant micromycetes. The pygidial secretion of C. coriaceus has showed antibacterial potential solely against Pseudomonas aeruginosa and antifungal activity against Aspergillus fumigatus, A. versicolor, A. ochraceus, and P. ochrochloron. Antibacterial properties of pygidial gland secretion of A. parallelepipedus were achieved against P. aeruginosa, while antifungal activity was detected against five of the eight tested micromycetes (A. fumigatus, A. versicolor, A. ochraceus, Trichoderma viride, and P. verrucosum var. cyclopium). Commercial antibiotics Streptomycin and Ampicillin and mycotics Ketoconazole and Bifonazole, applied as the positive controls, showed higher antibacterial/antifungal properties for all bacterial and fungal strains. The results of this observation might have a significant impact on the environmental aspects and possible medical purpose in the future.
PB  - Springer Nature
T2  - The Science of Nature
T1  - Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae)
IS  - 3-4
VL  - 103
DO  - 10.1007/s00114-016-1358-z
SP  - 34
ER  - 

@article{
author = "Nenadić, Marija and Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana and Perić Mataruga, Vesna and Ilijin, Larisa and Tešević, Vele and Vujisić, Ljubodrag and Todosijević, Marina and Vesović, Nikola and Ćurčić, Srećko",
year = "2016",
abstract = "The antimicrobial properties of the pygidial gland secretions released by the adults of the three ground beetle species, Carabus ullrichii, C. coriaceus, and Abax parallelepipedus, have been tested. Microdilution method was applied for detection of minimal inhibitory concentrations (MICs), minimal bactericidal concentrations (MBCs), and minimal fungicidal concentrations (MFCs). Additionally, morpho-histology of the pygidial glands is investigated. We have tested 16 laboratory and clinical strains of human pathogens-eight bacterial both gram-positive and gram-negative species and eight fungal species. The pygidial secretion samples of C. ullrichii have showed the strongest antimicrobial effect against all strains of treated bacteria and fungi. Staphylococcus aureus, Lysteria monocytogenes, and Salmonella typhimurium proved to be the most sensitive bacterial strains. Penicillium funiculosum proved to be the most sensitive micromycete, while P. ochrochloron and P. verrucosum var. cyclopium the most resistant micromycetes. The pygidial secretion of C. coriaceus has showed antibacterial potential solely against Pseudomonas aeruginosa and antifungal activity against Aspergillus fumigatus, A. versicolor, A. ochraceus, and P. ochrochloron. Antibacterial properties of pygidial gland secretion of A. parallelepipedus were achieved against P. aeruginosa, while antifungal activity was detected against five of the eight tested micromycetes (A. fumigatus, A. versicolor, A. ochraceus, Trichoderma viride, and P. verrucosum var. cyclopium). Commercial antibiotics Streptomycin and Ampicillin and mycotics Ketoconazole and Bifonazole, applied as the positive controls, showed higher antibacterial/antifungal properties for all bacterial and fungal strains. The results of this observation might have a significant impact on the environmental aspects and possible medical purpose in the future.",
publisher = "Springer Nature",
journal = "The Science of Nature",
title = "Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae)",
number = "3-4",
volume = "103",
doi = "10.1007/s00114-016-1358-z",
pages = "34"
}

Nenadić, M., Soković, M., Glamočlija, J., Ćirić, A., Perić Mataruga, V., Ilijin, L., Tešević, V., Vujisić, L., Todosijević, M., Vesović, N.,& Ćurčić, S.. (2016). Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae). in The Science of Nature
Springer Nature., 103(3-4), 34.
https://doi.org/10.1007/s00114-016-1358-z

Nenadić M, Soković M, Glamočlija J, Ćirić A, Perić Mataruga V, Ilijin L, Tešević V, Vujisić L, Todosijević M, Vesović N, Ćurčić S. Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae). in The Science of Nature. 2016;103(3-4):34.
doi:10.1007/s00114-016-1358-z .

Nenadić, Marija, Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, Perić Mataruga, Vesna, Ilijin, Larisa, Tešević, Vele, Vujisić, Ljubodrag, Todosijević, Marina, Vesović, Nikola, Ćurčić, Srećko, "Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae)" in The Science of Nature, 103, no. 3-4 (2016):34,
https://doi.org/10.1007/s00114-016-1358-z . .

TY  - JOUR
AU  - Nenadić, Marija
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Perić Mataruga, Vesna
AU  - Tešević, Vele
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Vesović, Nikola
AU  - Ćurčić, Srećko
PY  - 2016
UR  - https://www.cambridge.org/core/journals/bulletin-of-entomological-research/article/antimicrobial-activity-of-the-pygidial-gland-secretion-of-the-troglophilic-ground-beetle-laemostenus-pristonychus-punctatus-dejean-1828-insecta-coleoptera-carabidae/8DF8DE1725619065F0C0C1631B5CF359
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3970
AB  - The antimicrobial activity of the pygidial gland secretion released by adult individuals
of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean,
1828), applying microdilution method with the aim to detect minimal inhibitory concentration,
minimal bactericidal concentration and minimal fungicidal concentration,
has been investigated. In addition, morphology of the pygidial glands is observed.
Wehave tested 16 laboratory and clinical strains of human pathogens – eight bacterial
both gram-positive and gram-negative species and eight fungal species. The pygidial
secretion samples have showed antimicrobial properties against all strains of treated
bacteria and fungi. Micrococcus flavus proved to be more resistant compared with
other bacterial strains. More significant antimicrobial properties of the secretion are
observed against Escherichia coli, which proved to be the most sensitive bacteria.
Aspergillus fumigatus proved to be the most resistant, while Penicillium ochrochloron and
Penicillium verrucosum var. cyclopium themost sensitive micromycetes. Commercial antibiotics
Streptomycin and Ampicillin and antimycotics Ketoconazole and Bifonazole, applied
as positive controls, showed higher antibacterial properties for all bacterial and
fungal strains, except for P. ochrochloron, which proved to be more resistant on
Ketoconazole compared with the pygidial gland secretion of L. (P.) punctatus. Apart
from the role in ecological aspects, the antimicrobial properties of the tested secretion
possibly might have medical significance in the future.
PB  - Cambridge University Press
T2  - Bulletin of Entomological Research
T1  - Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae)
IS  - 4
VL  - 106
DO  - 10.1017/S0007485316000109
SP  - 474
EP  - 480
ER  - 

@article{
author = "Nenadić, Marija and Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana and Perić Mataruga, Vesna and Tešević, Vele and Vujisić, Ljubodrag and Todosijević, Marina and Vesović, Nikola and Ćurčić, Srećko",
year = "2016",
abstract = "The antimicrobial activity of the pygidial gland secretion released by adult individuals
of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean,
1828), applying microdilution method with the aim to detect minimal inhibitory concentration,
minimal bactericidal concentration and minimal fungicidal concentration,
has been investigated. In addition, morphology of the pygidial glands is observed.
Wehave tested 16 laboratory and clinical strains of human pathogens – eight bacterial
both gram-positive and gram-negative species and eight fungal species. The pygidial
secretion samples have showed antimicrobial properties against all strains of treated
bacteria and fungi. Micrococcus flavus proved to be more resistant compared with
other bacterial strains. More significant antimicrobial properties of the secretion are
observed against Escherichia coli, which proved to be the most sensitive bacteria.
Aspergillus fumigatus proved to be the most resistant, while Penicillium ochrochloron and
Penicillium verrucosum var. cyclopium themost sensitive micromycetes. Commercial antibiotics
Streptomycin and Ampicillin and antimycotics Ketoconazole and Bifonazole, applied
as positive controls, showed higher antibacterial properties for all bacterial and
fungal strains, except for P. ochrochloron, which proved to be more resistant on
Ketoconazole compared with the pygidial gland secretion of L. (P.) punctatus. Apart
from the role in ecological aspects, the antimicrobial properties of the tested secretion
possibly might have medical significance in the future.",
publisher = "Cambridge University Press",
journal = "Bulletin of Entomological Research",
title = "Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae)",
number = "4",
volume = "106",
doi = "10.1017/S0007485316000109",
pages = "474-480"
}

Nenadić, M., Soković, M., Glamočlija, J., Ćirić, A., Perić Mataruga, V., Tešević, V., Vujisić, L., Todosijević, M., Vesović, N.,& Ćurčić, S.. (2016). Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae). in Bulletin of Entomological Research
Cambridge University Press., 106(4), 474-480.
https://doi.org/10.1017/S0007485316000109

Nenadić M, Soković M, Glamočlija J, Ćirić A, Perić Mataruga V, Tešević V, Vujisić L, Todosijević M, Vesović N, Ćurčić S. Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae). in Bulletin of Entomological Research. 2016;106(4):474-480.
doi:10.1017/S0007485316000109 .

Nenadić, Marija, Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, Perić Mataruga, Vesna, Tešević, Vele, Vujisić, Ljubodrag, Todosijević, Marina, Vesović, Nikola, Ćurčić, Srećko, "Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae)" in Bulletin of Entomological Research, 106, no. 4 (2016):474-480,
https://doi.org/10.1017/S0007485316000109 . .

TY  - CONF
AU  - Pavlović, Danica
AU  - Petković, Branka
AU  - Ćurčić, Srećko
AU  - Todorović, Dajana
AU  - Vesović, Nikola
AU  - Pantelić, Dejan
AU  - Perić Mataruga, Vesna
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4025
AB  - Uticaj magnetnog polja na žive sisteme od velikog je značaja i predmet je intenzivnog ispitivanja, naročito poslednjih godina u eri sve većeg elektromagnetnog zagađenja. Laemostenus punctatus (Dejean, 1828) je vrsta troglofilnog tvrdokrilca iz porodice trčuljaka (fam. Carabidae). Cilj ove studije je bio da se ispita efekat statičkog magnetnog polja na različite parametre ponašanja (pređeni put, prosečna brzina, pokreti glave, rotacije tela, vreme kretanja i vreme mirovanja) jedinki L. punctatus. U eksperimentu su korišćene adultne jedinke (n = 17) ove vrste izlovljene u martu 2015. godine u Ogoreličkoj pećini, selo Sićevo, u blizini Niša, jugoistočna Srbija. Jedinke su bile podeljene u dve grupe: kontrolna (n = 9) i eksperimentalna (n = 8). Eksperimentalna grupa jedinki izlagana je statičkom magnetnom polju (110 mT) u trajanju od 5 sati. Ponašanje kontrolnih i tretiranih jedinki, neposredno nakon izlaganja, je praćeno u „open field“ testu u trajanju od 12 minuta i automatski analizirano korišćenjem Any-maze programa. Rezultati su grafički prikazani u intervalima od po 4 minuta. Pokazano je da izlaganje statičkom magnetnom polju povećava motornu aktivnost (pređeni put, prosečna brzina, pokreti glave, rotacije tela i vreme kretanja) i stoga smanjuje vreme mirovanja jedinki L. punctatus. Najznačajnije promene za sve navedene parametre uočene su u prvih 4 minuta, pri čemu je statistička značajnost utvrđena za sledeća dva parametra: pređeni put i prosečna brzina (p<0,05; Mann-Whitney U test). Na osnovu dobijenih rezultata možemo zaključiti da statičko magnetno polje utiče na ponašanje adultnih jedinki vrste L. punctatus, odnosno da deluje na centre odgovorne za kontrolu motorne aktivnosti.
PB  - Belgrade: Entomological Society of Serbia
C3  - Simpozijum entomologa Srbije 2015
T1  - Hipermotorno ponašanje vrste Laemostenus punctatus (Dejean, 1828) (Coleoptera: Carabidae) izazvano statičkim magnetnim poljem
SP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4025
ER  - 

@conference{
author = "Pavlović, Danica and Petković, Branka and Ćurčić, Srećko and Todorović, Dajana and Vesović, Nikola and Pantelić, Dejan and Perić Mataruga, Vesna",
year = "2015",
abstract = "Uticaj magnetnog polja na žive sisteme od velikog je značaja i predmet je intenzivnog ispitivanja, naročito poslednjih godina u eri sve većeg elektromagnetnog zagađenja. Laemostenus punctatus (Dejean, 1828) je vrsta troglofilnog tvrdokrilca iz porodice trčuljaka (fam. Carabidae). Cilj ove studije je bio da se ispita efekat statičkog magnetnog polja na različite parametre ponašanja (pređeni put, prosečna brzina, pokreti glave, rotacije tela, vreme kretanja i vreme mirovanja) jedinki L. punctatus. U eksperimentu su korišćene adultne jedinke (n = 17) ove vrste izlovljene u martu 2015. godine u Ogoreličkoj pećini, selo Sićevo, u blizini Niša, jugoistočna Srbija. Jedinke su bile podeljene u dve grupe: kontrolna (n = 9) i eksperimentalna (n = 8). Eksperimentalna grupa jedinki izlagana je statičkom magnetnom polju (110 mT) u trajanju od 5 sati. Ponašanje kontrolnih i tretiranih jedinki, neposredno nakon izlaganja, je praćeno u „open field“ testu u trajanju od 12 minuta i automatski analizirano korišćenjem Any-maze programa. Rezultati su grafički prikazani u intervalima od po 4 minuta. Pokazano je da izlaganje statičkom magnetnom polju povećava motornu aktivnost (pređeni put, prosečna brzina, pokreti glave, rotacije tela i vreme kretanja) i stoga smanjuje vreme mirovanja jedinki L. punctatus. Najznačajnije promene za sve navedene parametre uočene su u prvih 4 minuta, pri čemu je statistička značajnost utvrđena za sledeća dva parametra: pređeni put i prosečna brzina (p<0,05; Mann-Whitney U test). Na osnovu dobijenih rezultata možemo zaključiti da statičko magnetno polje utiče na ponašanje adultnih jedinki vrste L. punctatus, odnosno da deluje na centre odgovorne za kontrolu motorne aktivnosti.",
publisher = "Belgrade: Entomological Society of Serbia",
journal = "Simpozijum entomologa Srbije 2015",
title = "Hipermotorno ponašanje vrste Laemostenus punctatus (Dejean, 1828) (Coleoptera: Carabidae) izazvano statičkim magnetnim poljem",
pages = "17",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4025"
}

Pavlović, D., Petković, B., Ćurčić, S., Todorović, D., Vesović, N., Pantelić, D.,& Perić Mataruga, V.. (2015). Hipermotorno ponašanje vrste Laemostenus punctatus (Dejean, 1828) (Coleoptera: Carabidae) izazvano statičkim magnetnim poljem. in Simpozijum entomologa Srbije 2015
Belgrade: Entomological Society of Serbia., 17.
https://hdl.handle.net/21.15107/rcub_ibiss_4025

Pavlović D, Petković B, Ćurčić S, Todorović D, Vesović N, Pantelić D, Perić Mataruga V. Hipermotorno ponašanje vrste Laemostenus punctatus (Dejean, 1828) (Coleoptera: Carabidae) izazvano statičkim magnetnim poljem. in Simpozijum entomologa Srbije 2015. 2015;:17.
https://hdl.handle.net/21.15107/rcub_ibiss_4025 .

Pavlović, Danica, Petković, Branka, Ćurčić, Srećko, Todorović, Dajana, Vesović, Nikola, Pantelić, Dejan, Perić Mataruga, Vesna, "Hipermotorno ponašanje vrste Laemostenus punctatus (Dejean, 1828) (Coleoptera: Carabidae) izazvano statičkim magnetnim poljem" in Simpozijum entomologa Srbije 2015 (2015):17,
https://hdl.handle.net/21.15107/rcub_ibiss_4025 .