TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Milinčić, Danijel
AU  - Špirović Trifunović, Bojana
AU  - Nedić, Nebojša
AU  - Gašić, Uroš
AU  - Tešić, Živoslav
AU  - Stanojević, Sladjana
AU  - Pešić, Mirjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6466
AB  - The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties of bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, a lipid fraction was prepared for quantifying fatty acids using GC-FID. Extractable and alkaline-hydrolysable PBP fractions (obtained from a defatted sample) were used to determine the qualitative and quantitative profiles of phenolic compounds, phenylamides and alkaloids using UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) were conducted to evaluate the antioxidant properties. Phenolic compounds were more present in the extractable fraction than in the alkaline-hydrolysable fraction. Luteolin was the predominant compound in the extractable fraction, followed by tricetin and various derivatives of kaempferol. This study presents one of the first reports on the quantification of tricetin aglycone outside the Myrtaceae plant family. The alkaline-hydrolysable fraction exhibited a different phenolic profile, with a significantly lower amount of phenolics. Kaempferol/derivatives, specific compounds like ferulic and 5-carboxyvanillic acids, and (epi)catechin 3-O-gallate were the predominant compounds in this fraction. Regarding phenylamides, the extractable fraction demonstrated a diverse range of these bioactive compounds, with a notable abundance of different spermine derivatives. In contrast, the hydrolysable fraction contained six spermine derivatives and one spermidine derivative. The examined fractions also revealed the presence of seventeen different alkaloids, belonging to the benzylisoquinoline, berberine and isoquinoline classes. The fatty-acid profile confirmed the prevalence of unsaturated fatty acids. Furthermore, both fractions exhibited significant antioxidant activity, with the extractable fraction showing particularly high activity. Among the assays conducted, the CUPRAC assay highlighted the exceptional ability of PBP’s bioactive compounds to reduce cupric ions.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties
IS  - 7
VL  - 12
DO  - 10.3390/antiox12071424
SP  - 1424
ER  - 

@article{
author = "Kostić, Aleksandar and Milinčić, Danijel and Špirović Trifunović, Bojana and Nedić, Nebojša and Gašić, Uroš and Tešić, Živoslav and Stanojević, Sladjana and Pešić, Mirjana",
year = "2023",
abstract = "The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties of bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, a lipid fraction was prepared for quantifying fatty acids using GC-FID. Extractable and alkaline-hydrolysable PBP fractions (obtained from a defatted sample) were used to determine the qualitative and quantitative profiles of phenolic compounds, phenylamides and alkaloids using UHPLC/Q-ToF-MS. Additionally, various spectrophotometric assays (TAC, FRP, CUPRAC, DPPH⦁) were conducted to evaluate the antioxidant properties. Phenolic compounds were more present in the extractable fraction than in the alkaline-hydrolysable fraction. Luteolin was the predominant compound in the extractable fraction, followed by tricetin and various derivatives of kaempferol. This study presents one of the first reports on the quantification of tricetin aglycone outside the Myrtaceae plant family. The alkaline-hydrolysable fraction exhibited a different phenolic profile, with a significantly lower amount of phenolics. Kaempferol/derivatives, specific compounds like ferulic and 5-carboxyvanillic acids, and (epi)catechin 3-O-gallate were the predominant compounds in this fraction. Regarding phenylamides, the extractable fraction demonstrated a diverse range of these bioactive compounds, with a notable abundance of different spermine derivatives. In contrast, the hydrolysable fraction contained six spermine derivatives and one spermidine derivative. The examined fractions also revealed the presence of seventeen different alkaloids, belonging to the benzylisoquinoline, berberine and isoquinoline classes. The fatty-acid profile confirmed the prevalence of unsaturated fatty acids. Furthermore, both fractions exhibited significant antioxidant activity, with the extractable fraction showing particularly high activity. Among the assays conducted, the CUPRAC assay highlighted the exceptional ability of PBP’s bioactive compounds to reduce cupric ions.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties",
number = "7",
volume = "12",
doi = "10.3390/antiox12071424",
pages = "1424"
}

Kostić, A., Milinčić, D., Špirović Trifunović, B., Nedić, N., Gašić, U., Tešić, Ž., Stanojević, S.,& Pešić, M.. (2023). Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties. in Antioxidants
Basel: MDPI., 12(7), 1424.
https://doi.org/10.3390/antiox12071424

Kostić A, Milinčić D, Špirović Trifunović B, Nedić N, Gašić U, Tešić Ž, Stanojević S, Pešić M. Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties. in Antioxidants. 2023;12(7):1424.
doi:10.3390/antiox12071424 .

Kostić, Aleksandar, Milinčić, Danijel, Špirović Trifunović, Bojana, Nedić, Nebojša, Gašić, Uroš, Tešić, Živoslav, Stanojević, Sladjana, Pešić, Mirjana, "Monofloral Corn Poppy Bee-Collected Pollen—A Detailed Insight into Its Phytochemical Composition and Antioxidant Properties" in Antioxidants, 12, no. 7 (2023):1424,
https://doi.org/10.3390/antiox12071424 . .

TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Mačukanović-Jocić, Marina
AU  - Radošević, Radenko
AU  - Nedić, Nebojša
AU  - Gašić, Uroš
AU  - Tosti, Tomislav
AU  - Tešić, Živoslav
AU  - Meland, Mekjell
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6306
AB  - The topography and morpho-anatomical characteristics of floral nectaries and the chemical analysis of nectar have been studied in seven pear cultivars with different susceptibility to Erwinia amylovora. The susceptible cultivar Williams, the moderately resistant cultivars Bella di guigno, Poire de Cure and the low susceptible cultivar Alexander Lucas originated from Pyrus communis, while highly resistant cultivars Chojuro and Nijisseiki from P. pyraster and Kieffer as interspecies hybrid P. communis × P. pyraster were included in this experiment and studied for the first time. Large differences in size and structure of the nectaries were observed in these seven pear cultivars. The epidermal cells were with cuticle being more or less striated in Alexander Lucas, Kieffer and Williams. Resistant cultivars had a narrow, elongated cell shape of epidermal cells while those susceptible had an isodiametric. Stomata were mesomorphic in all cultivars except in Poire de Cure and Williams, being slightly xeromorphic since they were situated in deep hollows. Guard cells of the modified stomata were much larger in resistant cultivars. Hypanthium cells were larger in resistant compared to susceptible cultivars. The most abundant sugars were glucose, fructose, sorbitol and sucrose in nectar of all studied pear cultivars. The resistant cultivars (Chojuro, Kieffer and Nijisseiki) had a ~2-fold higher level of sorbitol and galactose, ~2.2-fold higher isomaltose, ~2.7-fold turanose, ~3.35-fold maltose, ~4.4-fold melibiose and ~12.7-fold higher melesitose compared to susceptible cultivars. The sum of quantified phenolic acids varied from 0.049 (Williams) up to 4.074 µg CAE/mL (Kieffer), while flavonoid glycosides levels ranged from 1.224 (Williams) up to 11.686 µg RE/mL (Nijisseiki). In the nectar of the resistant cultivars, rutin, apigetrin, together with patuletin and luteolin glycosides were detected but not in susceptible cultivars, which could be considered as the markers of resistance.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora
IS  - 4
VL  - 9
DO  - 10.3390/horticulturae9040424
SP  - 424
ER  - 

@article{
author = "Fotirić Akšić, Milica and Mačukanović-Jocić, Marina and Radošević, Radenko and Nedić, Nebojša and Gašić, Uroš and Tosti, Tomislav and Tešić, Živoslav and Meland, Mekjell",
year = "2023",
abstract = "The topography and morpho-anatomical characteristics of floral nectaries and the chemical analysis of nectar have been studied in seven pear cultivars with different susceptibility to Erwinia amylovora. The susceptible cultivar Williams, the moderately resistant cultivars Bella di guigno, Poire de Cure and the low susceptible cultivar Alexander Lucas originated from Pyrus communis, while highly resistant cultivars Chojuro and Nijisseiki from P. pyraster and Kieffer as interspecies hybrid P. communis × P. pyraster were included in this experiment and studied for the first time. Large differences in size and structure of the nectaries were observed in these seven pear cultivars. The epidermal cells were with cuticle being more or less striated in Alexander Lucas, Kieffer and Williams. Resistant cultivars had a narrow, elongated cell shape of epidermal cells while those susceptible had an isodiametric. Stomata were mesomorphic in all cultivars except in Poire de Cure and Williams, being slightly xeromorphic since they were situated in deep hollows. Guard cells of the modified stomata were much larger in resistant cultivars. Hypanthium cells were larger in resistant compared to susceptible cultivars. The most abundant sugars were glucose, fructose, sorbitol and sucrose in nectar of all studied pear cultivars. The resistant cultivars (Chojuro, Kieffer and Nijisseiki) had a ~2-fold higher level of sorbitol and galactose, ~2.2-fold higher isomaltose, ~2.7-fold turanose, ~3.35-fold maltose, ~4.4-fold melibiose and ~12.7-fold higher melesitose compared to susceptible cultivars. The sum of quantified phenolic acids varied from 0.049 (Williams) up to 4.074 µg CAE/mL (Kieffer), while flavonoid glycosides levels ranged from 1.224 (Williams) up to 11.686 µg RE/mL (Nijisseiki). In the nectar of the resistant cultivars, rutin, apigetrin, together with patuletin and luteolin glycosides were detected but not in susceptible cultivars, which could be considered as the markers of resistance.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora",
number = "4",
volume = "9",
doi = "10.3390/horticulturae9040424",
pages = "424"
}

Fotirić Akšić, M., Mačukanović-Jocić, M., Radošević, R., Nedić, N., Gašić, U., Tosti, T., Tešić, Ž.,& Meland, M.. (2023). The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora. in Horticulturae
Basel: MDPI., 9(4), 424.
https://doi.org/10.3390/horticulturae9040424

Fotirić Akšić M, Mačukanović-Jocić M, Radošević R, Nedić N, Gašić U, Tosti T, Tešić Ž, Meland M. The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora. in Horticulturae. 2023;9(4):424.
doi:10.3390/horticulturae9040424 .

Fotirić Akšić, Milica, Mačukanović-Jocić, Marina, Radošević, Radenko, Nedić, Nebojša, Gašić, Uroš, Tosti, Tomislav, Tešić, Živoslav, Meland, Mekjell, "The Morpho-Anatomy of Nectaries and Chemical Composition of Nectar in Pear Cultivars with Different Susceptibility to Erwinia amlylovora" in Horticulturae, 9, no. 4 (2023):424,
https://doi.org/10.3390/horticulturae9040424 . .

TY  - JOUR
AU  - Nedić, Nebojša
AU  - Nešović, Milica
AU  - Radišić, Predrag
AU  - Gašić, Uroš
AU  - Baošić, Rada
AU  - Joksimović, Kristina
AU  - Pezo, Lato
AU  - Tešić, Živoslav
AU  - Vovk, Irena
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fnut.2022.941463/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9263698
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5080
AB  - This study presents a detailed characterization of 27 honey samples from the Tara Mountain region in Serbia using different comprehensive techniques and methods. The types of the honey samples were defined as monofloral (4 samples), honeydew (5 samples) and polyfloral (18 samples) honey based on determined polyphenol content, antioxidant activity, electrical conductivity and melissopalynological analyses. Physicochemical parameters such as pH (4.13-4.94), diastase activity (24.20-41.70 DN), acidity (14.60-29.70 meq/kg), content of 5-(hydroxymethyl)furfural (in range below 5, up to 16.90 mg/kg), sucrose (0.20-3.90 g/100 g), and moisture content (15.01-19.23%) confirmed the required quality of the honey samples. Sensory analysis revealed honey characteristics favorable to consumers. Analyses of 19 phenolic compounds using ultra-high-performance liquid chromatography with a diode-array detection and triple quadrupole mass spectrometry (UHPLC-DAD-MS/MS) revealed six phenolic acids and 13 other compounds from the group of flavonoids and their glycosides. In all the samples the highest content was determined for p-coumaric acid, followed by caffeic acid and pinocembrin. Besides total phenolic content and radical scavenging activity, antimicrobial activity was also examined. Most honey samples showed bactericidal activity against Staphylococcus aureus and bacteriostatic activity against Escherichia coli, while none of the honey samples inhibited the growth of Candida albicans. Chemometric analyses were applied for an in-depth study of the results to further evaluate the characteristics of the honey samples studied. Principal component analysis (PCA) was used for assessing the differences in physicochemical parameters, polyphenols content and antioxidant capacity between honey samples. The unrooted cluster tree was used to group the samples based on the melissopalynological analyses.
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Nutrition
T1  - Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.
VL  - 9
DO  - 10.3389/fnut.2022.941463
SP  - 941463
ER  - 

@article{
author = "Nedić, Nebojša and Nešović, Milica and Radišić, Predrag and Gašić, Uroš and Baošić, Rada and Joksimović, Kristina and Pezo, Lato and Tešić, Živoslav and Vovk, Irena",
year = "2022",
abstract = "This study presents a detailed characterization of 27 honey samples from the Tara Mountain region in Serbia using different comprehensive techniques and methods. The types of the honey samples were defined as monofloral (4 samples), honeydew (5 samples) and polyfloral (18 samples) honey based on determined polyphenol content, antioxidant activity, electrical conductivity and melissopalynological analyses. Physicochemical parameters such as pH (4.13-4.94), diastase activity (24.20-41.70 DN), acidity (14.60-29.70 meq/kg), content of 5-(hydroxymethyl)furfural (in range below 5, up to 16.90 mg/kg), sucrose (0.20-3.90 g/100 g), and moisture content (15.01-19.23%) confirmed the required quality of the honey samples. Sensory analysis revealed honey characteristics favorable to consumers. Analyses of 19 phenolic compounds using ultra-high-performance liquid chromatography with a diode-array detection and triple quadrupole mass spectrometry (UHPLC-DAD-MS/MS) revealed six phenolic acids and 13 other compounds from the group of flavonoids and their glycosides. In all the samples the highest content was determined for p-coumaric acid, followed by caffeic acid and pinocembrin. Besides total phenolic content and radical scavenging activity, antimicrobial activity was also examined. Most honey samples showed bactericidal activity against Staphylococcus aureus and bacteriostatic activity against Escherichia coli, while none of the honey samples inhibited the growth of Candida albicans. Chemometric analyses were applied for an in-depth study of the results to further evaluate the characteristics of the honey samples studied. Principal component analysis (PCA) was used for assessing the differences in physicochemical parameters, polyphenols content and antioxidant capacity between honey samples. The unrooted cluster tree was used to group the samples based on the melissopalynological analyses.",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Nutrition",
title = "Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.",
volume = "9",
doi = "10.3389/fnut.2022.941463",
pages = "941463"
}

Nedić, N., Nešović, M., Radišić, P., Gašić, U., Baošić, R., Joksimović, K., Pezo, L., Tešić, Ž.,& Vovk, I.. (2022). Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.. in Frontiers in Nutrition
Lausanne: Frontiers Media S.A.., 9, 941463.
https://doi.org/10.3389/fnut.2022.941463

Nedić N, Nešović M, Radišić P, Gašić U, Baošić R, Joksimović K, Pezo L, Tešić Ž, Vovk I. Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.. in Frontiers in Nutrition. 2022;9:941463.
doi:10.3389/fnut.2022.941463 .

Nedić, Nebojša, Nešović, Milica, Radišić, Predrag, Gašić, Uroš, Baošić, Rada, Joksimović, Kristina, Pezo, Lato, Tešić, Živoslav, Vovk, Irena, "Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia." in Frontiers in Nutrition, 9 (2022):941463,
https://doi.org/10.3389/fnut.2022.941463 . .

TY  - JOUR
AU  - Đorđević, Sofija
AU  - Nedić, Nebojša
AU  - Pavlović, Aleksandra
AU  - Milojković-Opsenica, Dušanka
AU  - Tešić, Živoslav
AU  - Gašić, Uroš
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S2210803322000495
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5012
AB  - Introduction The aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value. Methods For determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction. Results The extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg). Conclusions Honey, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.
T2  - Journal of Herbal Medicine
T1  - Honey with added value – enriched with rutin and quercetin from Sophora flower
VL  - 34
DO  - 10.1016/j.hermed.2022.100580
SP  - 100580
ER  - 

@article{
author = "Đorđević, Sofija and Nedić, Nebojša and Pavlović, Aleksandra and Milojković-Opsenica, Dušanka and Tešić, Živoslav and Gašić, Uroš",
year = "2022",
abstract = "Introduction The aim of this study was to find the best conditions for the extraction of rutin and quercetin from Sophora flower in acacia honey as an extractant, and to formulate a new product – honey with added value. Methods For determination of the optimal extraction conditions of the above mentioned compounds, 10% m/m of the dried Sophora flower was added to honey and the concentration of rutin and quercetin was monitored, at 4 °C and room temperature at 1, 15, 40 and 90 day intervals. The analyses of rutin and quercetin content in honey were performed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The content of rutin and quercetin in the flower was also determined to check the quality of the herbal drug used, as well as to evaluate the effectiveness of the extraction. Results The extraction of rutin and quercetin, in a honey sample using 10% of the Sophora flower, stored at room temperature, for 40 days, was the most intense one (150.24 mg/kg of rutin and 1338.93 mg/kg of quercetin). Sophora flower corresponds to the pharmacopoeia requirements for the quality of an herbal drug. The concentrations of rutin and quercetin in acacia honey were 0.08 and 0.14 mg/kg, respectively. The concentration of rutin in honey from the melliferous pasture of the Sophora flower was 0.40 mg/kg, while quercetin was below the detection limit (0.1 mg/kg). Conclusions Honey, enriched with rutin and quercetin from Sophora flower, had sensory acceptability and pleasant taste and aroma.",
journal = "Journal of Herbal Medicine",
title = "Honey with added value – enriched with rutin and quercetin from Sophora flower",
volume = "34",
doi = "10.1016/j.hermed.2022.100580",
pages = "100580"
}

Đorđević, S., Nedić, N., Pavlović, A., Milojković-Opsenica, D., Tešić, Ž.,& Gašić, U.. (2022). Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine, 34, 100580.
https://doi.org/10.1016/j.hermed.2022.100580

Đorđević S, Nedić N, Pavlović A, Milojković-Opsenica D, Tešić Ž, Gašić U. Honey with added value – enriched with rutin and quercetin from Sophora flower. in Journal of Herbal Medicine. 2022;34:100580.
doi:10.1016/j.hermed.2022.100580 .

Đorđević, Sofija, Nedić, Nebojša, Pavlović, Aleksandra, Milojković-Opsenica, Dušanka, Tešić, Živoslav, Gašić, Uroš, "Honey with added value – enriched with rutin and quercetin from Sophora flower" in Journal of Herbal Medicine, 34 (2022):100580,
https://doi.org/10.1016/j.hermed.2022.100580 . .

TY  - JOUR
AU  - Nešović, Milica
AU  - Gašić, Uroš
AU  - Tosti, Tomislav
AU  - Horvacki, Nikola
AU  - Nedić, Nebojša
AU  - Sredojević, Milica
AU  - Blagojević, Stevan
AU  - Ignjatović, Ljubiša
AU  - Tešić, Živoslav
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4314
AB  - The aim of this study was to provide information on the phenolic and sugar profiles of different parts of the buckwheat plant, which can define that buckwheat is a functional food, with a high nutritional value and very useful for human health. Therefore, the extracts of buckwheat leaf, stem, and flower, as well as buckwheat grain were analysed for the content of polyphenol and antioxidant tests. The identification of a notable number of phenolic compounds and quantification of sugars in different parts of buckwheat indicates that buckwheat is a highly valuable plant. A total of 60 phenolic compounds were identified (18 cinnamic acid derivatives, 14 flavonols, 13 flavan-3-ols (including proanthocyanidins), 10 hydroxybenzoic acid derivatives, and 5 flavones) using ultra-high-performance liquid chromatography (UHPLC), coupled with a hybrid mass spectrometer which combines the Linear Trap Quadrupole (LTQ) and OrbiTrap mass analyzer. The highest number of phenolic compounds was found in the analysed buckwheat flower sample, and then in the leaf, followed by the grain and the stem. In addition, the sugar profile of buckwheat leaf, stem, flower and grain, as well as the buckwheat pollen and the nectar was analysed. Hence, 16 sugars and 5 sugar alcohols were detected by the high-performance anion exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD). Sucrose was found in a significant amount with the highest content in buckwheat leaf. Trisaccharides had similar accumulation in the sample extracts, while disaccharides dominated in buckwheat leaf, followed by nectar and pollen. The sugar alcohols showed the highest content in buckwheat grain, where erythritol was predominant. The obtained results show that buckwheat is very rich in phenolic compounds and sugars. In addition to grain, the other parts of the buckwheat plant can be used as a very good source of different classes of phenolic compounds. This study provides useful information on the distribution of phytochemicals in different parts of the buckwheat plant, which contribute to the maintaining of the status of buckwheat as a functional food.
PB  - Cambridge: Royal Society of Chemistry
T2  - RSC Advances
T1  - Distribution of polyphenolic and sugar compounds in different buckwheat plant parts
IS  - 42
VL  - 11
DO  - 10.1039/D1RA04250E
SP  - 25816
EP  - 25829
ER  - 

@article{
author = "Nešović, Milica and Gašić, Uroš and Tosti, Tomislav and Horvacki, Nikola and Nedić, Nebojša and Sredojević, Milica and Blagojević, Stevan and Ignjatović, Ljubiša and Tešić, Živoslav",
year = "2021",
abstract = "The aim of this study was to provide information on the phenolic and sugar profiles of different parts of the buckwheat plant, which can define that buckwheat is a functional food, with a high nutritional value and very useful for human health. Therefore, the extracts of buckwheat leaf, stem, and flower, as well as buckwheat grain were analysed for the content of polyphenol and antioxidant tests. The identification of a notable number of phenolic compounds and quantification of sugars in different parts of buckwheat indicates that buckwheat is a highly valuable plant. A total of 60 phenolic compounds were identified (18 cinnamic acid derivatives, 14 flavonols, 13 flavan-3-ols (including proanthocyanidins), 10 hydroxybenzoic acid derivatives, and 5 flavones) using ultra-high-performance liquid chromatography (UHPLC), coupled with a hybrid mass spectrometer which combines the Linear Trap Quadrupole (LTQ) and OrbiTrap mass analyzer. The highest number of phenolic compounds was found in the analysed buckwheat flower sample, and then in the leaf, followed by the grain and the stem. In addition, the sugar profile of buckwheat leaf, stem, flower and grain, as well as the buckwheat pollen and the nectar was analysed. Hence, 16 sugars and 5 sugar alcohols were detected by the high-performance anion exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD). Sucrose was found in a significant amount with the highest content in buckwheat leaf. Trisaccharides had similar accumulation in the sample extracts, while disaccharides dominated in buckwheat leaf, followed by nectar and pollen. The sugar alcohols showed the highest content in buckwheat grain, where erythritol was predominant. The obtained results show that buckwheat is very rich in phenolic compounds and sugars. In addition to grain, the other parts of the buckwheat plant can be used as a very good source of different classes of phenolic compounds. This study provides useful information on the distribution of phytochemicals in different parts of the buckwheat plant, which contribute to the maintaining of the status of buckwheat as a functional food.",
publisher = "Cambridge: Royal Society of Chemistry",
journal = "RSC Advances",
title = "Distribution of polyphenolic and sugar compounds in different buckwheat plant parts",
number = "42",
volume = "11",
doi = "10.1039/D1RA04250E",
pages = "25816-25829"
}

Nešović, M., Gašić, U., Tosti, T., Horvacki, N., Nedić, N., Sredojević, M., Blagojević, S., Ignjatović, L.,& Tešić, Ž.. (2021). Distribution of polyphenolic and sugar compounds in different buckwheat plant parts. in RSC Advances
Cambridge: Royal Society of Chemistry., 11(42), 25816-25829.
https://doi.org/10.1039/D1RA04250E

Nešović M, Gašić U, Tosti T, Horvacki N, Nedić N, Sredojević M, Blagojević S, Ignjatović L, Tešić Ž. Distribution of polyphenolic and sugar compounds in different buckwheat plant parts. in RSC Advances. 2021;11(42):25816-25829.
doi:10.1039/D1RA04250E .

Nešović, Milica, Gašić, Uroš, Tosti, Tomislav, Horvacki, Nikola, Nedić, Nebojša, Sredojević, Milica, Blagojević, Stevan, Ignjatović, Ljubiša, Tešić, Živoslav, "Distribution of polyphenolic and sugar compounds in different buckwheat plant parts" in RSC Advances, 11, no. 42 (2021):25816-25829,
https://doi.org/10.1039/D1RA04250E . .

TY  - JOUR
AU  - Kostić, Aleksandar
AU  - Milinčić, Danijel
AU  - Nedić, Nebojša
AU  - Gašić, Uroš
AU  - Špirović Trifunović, Bojana
AU  - Vojt, Denis
AU  - Tešić, Živoslav
AU  - Pešić, Mirjana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4315
AB  - The current study intended to determine, for the first time, phenolic and fatty acid profile, antioxidant and certain nutritional properties of monofloral bee-collected artichoke (Cynara scolymus) pollen. Based on UHPLC-DAD MS-MS analysis the main phenolics in extractable fraction were different flavonol glycosides (in particular Isorhamnetin-3-O-glucoside, 49.2 mg/kg of dry weight) while ferulic acid was the predominant phenolic compound (39.4 mg/kg of dry weight) in the alkaline hydrolyzable fraction. Among fatty acids (FAs), results of GC-FID analysis revealed prevalence of unsaturated FAs with cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and oleic acid as the main ones- 28.4% and 24.9%, respectively. Based on the FA composition, nutritional analysis proved that artichoke bee-collected pollen had balanced ω-6 and ω-3 FAs content. To determine the antioxidant properties of pollen, five different assays were applied. It was proved that bioactive compounds in artichoke pollen possessed significant ability to quench DPPH radical as well as ABTS radical cation. In addition, in vitro phosphomolybdenum assay confirmed that artichoke pollen is an excellent source of different antioxidants. Pollen extracts exhibited moderate ferric reducing power as well as low ferrous chelating ability. Some further antioxidant studies (preferably in vivo) should be performed to confirm the observed results.
PB  - Basel: MDPI
T2  - Antioxidants
T1  - Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen
IS  - 7
VL  - 10
DO  - 10.3390/antiox10071091
SP  - 1091
ER  - 

@article{
author = "Kostić, Aleksandar and Milinčić, Danijel and Nedić, Nebojša and Gašić, Uroš and Špirović Trifunović, Bojana and Vojt, Denis and Tešić, Živoslav and Pešić, Mirjana",
year = "2021",
abstract = "The current study intended to determine, for the first time, phenolic and fatty acid profile, antioxidant and certain nutritional properties of monofloral bee-collected artichoke (Cynara scolymus) pollen. Based on UHPLC-DAD MS-MS analysis the main phenolics in extractable fraction were different flavonol glycosides (in particular Isorhamnetin-3-O-glucoside, 49.2 mg/kg of dry weight) while ferulic acid was the predominant phenolic compound (39.4 mg/kg of dry weight) in the alkaline hydrolyzable fraction. Among fatty acids (FAs), results of GC-FID analysis revealed prevalence of unsaturated FAs with cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and oleic acid as the main ones- 28.4% and 24.9%, respectively. Based on the FA composition, nutritional analysis proved that artichoke bee-collected pollen had balanced ω-6 and ω-3 FAs content. To determine the antioxidant properties of pollen, five different assays were applied. It was proved that bioactive compounds in artichoke pollen possessed significant ability to quench DPPH radical as well as ABTS radical cation. In addition, in vitro phosphomolybdenum assay confirmed that artichoke pollen is an excellent source of different antioxidants. Pollen extracts exhibited moderate ferric reducing power as well as low ferrous chelating ability. Some further antioxidant studies (preferably in vivo) should be performed to confirm the observed results.",
publisher = "Basel: MDPI",
journal = "Antioxidants",
title = "Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen",
number = "7",
volume = "10",
doi = "10.3390/antiox10071091",
pages = "1091"
}

Kostić, A., Milinčić, D., Nedić, N., Gašić, U., Špirović Trifunović, B., Vojt, D., Tešić, Ž.,& Pešić, M.. (2021). Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen. in Antioxidants
Basel: MDPI., 10(7), 1091.
https://doi.org/10.3390/antiox10071091

Kostić A, Milinčić D, Nedić N, Gašić U, Špirović Trifunović B, Vojt D, Tešić Ž, Pešić M. Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen. in Antioxidants. 2021;10(7):1091.
doi:10.3390/antiox10071091 .

Kostić, Aleksandar, Milinčić, Danijel, Nedić, Nebojša, Gašić, Uroš, Špirović Trifunović, Bojana, Vojt, Denis, Tešić, Živoslav, Pešić, Mirjana, "Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen" in Antioxidants, 10, no. 7 (2021):1091,
https://doi.org/10.3390/antiox10071091 . .

TY  - JOUR
AU  - Nešović, Milica
AU  - Gašić, Uroš
AU  - Tosti, Tomislav
AU  - Horvacki, Nikola
AU  - Šikoparija, Branko
AU  - Nedić, Nebojša
AU  - Blagojević, Stevan
AU  - Ignjatović, Ljubiša
AU  - Tešić, Živoslav
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4058
AB  - A focus of research in recent years is the comparison of honey as the final product of bees with pollen and nectar of the plant from which the honey originates, as the main food source for bees. Buckwheat honey is recognized as a nutritionally valuable product, which provides a scientifically proven health benefit and is confirmed as a functional food. The quality of this type of honey is attributed to high levels of phytochemicals in buckwheat. The purpose of this study was the examination of similarity between buckwheat honey and buckwheat nectar and pollen, as well as simultaneous investigation of their chemical profiles and the origin of the honey. The phenolic profile of buckwheat pollen showed a lower number of flavonoids and phenolic acids than those of nectar and honey samples, but confirmed the presence of the most characteristic polyphenols derived from the buckwheat plant. The notable difference was found to be the presence of (epi)catechin units, its galloylated derivatives and procyanidin dimers, which were not present in honey. Honey polyphenols displayed a pronounced correlation with those of nectar, but not with those of pollen. Finally, by comparing the polyphenolic profiles of honey, nectar and pollen sharing the same geographical origin, new data could be provided for a potential assessment of the botanical origin of buckwheat honey.
PB  - Royal Society of Chemistry
T2  - Royal Society Open Science
T1  - Polyphenolic profile of buckwheat honey, nectar and pollen
IS  - 12
VL  - 7
DO  - 10.1098/rsos.201576
SP  - 201576
ER  - 

@article{
author = "Nešović, Milica and Gašić, Uroš and Tosti, Tomislav and Horvacki, Nikola and Šikoparija, Branko and Nedić, Nebojša and Blagojević, Stevan and Ignjatović, Ljubiša and Tešić, Živoslav",
year = "2020",
abstract = "A focus of research in recent years is the comparison of honey as the final product of bees with pollen and nectar of the plant from which the honey originates, as the main food source for bees. Buckwheat honey is recognized as a nutritionally valuable product, which provides a scientifically proven health benefit and is confirmed as a functional food. The quality of this type of honey is attributed to high levels of phytochemicals in buckwheat. The purpose of this study was the examination of similarity between buckwheat honey and buckwheat nectar and pollen, as well as simultaneous investigation of their chemical profiles and the origin of the honey. The phenolic profile of buckwheat pollen showed a lower number of flavonoids and phenolic acids than those of nectar and honey samples, but confirmed the presence of the most characteristic polyphenols derived from the buckwheat plant. The notable difference was found to be the presence of (epi)catechin units, its galloylated derivatives and procyanidin dimers, which were not present in honey. Honey polyphenols displayed a pronounced correlation with those of nectar, but not with those of pollen. Finally, by comparing the polyphenolic profiles of honey, nectar and pollen sharing the same geographical origin, new data could be provided for a potential assessment of the botanical origin of buckwheat honey.",
publisher = "Royal Society of Chemistry",
journal = "Royal Society Open Science",
title = "Polyphenolic profile of buckwheat honey, nectar and pollen",
number = "12",
volume = "7",
doi = "10.1098/rsos.201576",
pages = "201576"
}

Nešović, M., Gašić, U., Tosti, T., Horvacki, N., Šikoparija, B., Nedić, N., Blagojević, S., Ignjatović, L.,& Tešić, Ž.. (2020). Polyphenolic profile of buckwheat honey, nectar and pollen. in Royal Society Open Science
Royal Society of Chemistry., 7(12), 201576.
https://doi.org/10.1098/rsos.201576

Nešović M, Gašić U, Tosti T, Horvacki N, Šikoparija B, Nedić N, Blagojević S, Ignjatović L, Tešić Ž. Polyphenolic profile of buckwheat honey, nectar and pollen. in Royal Society Open Science. 2020;7(12):201576.
doi:10.1098/rsos.201576 .

Nešović, Milica, Gašić, Uroš, Tosti, Tomislav, Horvacki, Nikola, Šikoparija, Branko, Nedić, Nebojša, Blagojević, Stevan, Ignjatović, Ljubiša, Tešić, Živoslav, "Polyphenolic profile of buckwheat honey, nectar and pollen" in Royal Society Open Science, 7, no. 12 (2020):201576,
https://doi.org/10.1098/rsos.201576 . .