TY  - JOUR
AU  - Bugeja Douglas, Adrian
AU  - Nešović, Milica
AU  - Šikoparija, Branko
AU  - Radišić, Predrag
AU  - Tosti, Tomislav
AU  - Trifković, Jelena
AU  - Russi, Luigi
AU  - Attard, Everaldo
AU  - Tešić, Živoslav
AU  - Gašić, Uroš
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5665
AB  - Malta, a country renowned for its honey, has not been extensively mentioned in studies based on honey. In addition to many parameters, the collection period affects honey quality, precisely due to the different floral composition that exists during a certain season. Therefore, the significance of this study refers to the provision of data on honey from Malta collected during the autumn, spring, and summer seasons. Melissopalynological analysis, determination of physicochemical parameters, and the use of analytical chromatographic methods enabled detailed analysis of this honey. Principal component analysis (PCA) provided the differentiation of Maltese honey depending on the harvest season. Lotus pollen, followed by Eucalyptus, predominated in all honey samples. Characteristic compounds for summer honey were pinocembrin, galangin, kaempferol, chrysin, p-hydroxybenzoic acid, vanillic acid and maltotriose, while quercetin 3-O-galactoside, ferulic acid, ellagic acid, protocatechuic acid, luteolin 7-O-glucoside and melibiose were specific for autumn honey. A higher amount of p-coumaric acid, genistein, catechin, as well as the content of many sugars were found in spring samples. To the best of our knowledge, this is the first scientific work dealing with a detailed chemical analysis of Maltese honey.
PB  - Belgrade: Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Melissopalynology analysis, determination of physicochemical parameters, sugars and phenolics in Maltese honey collected in different seasons
IS  - 9
VL  - 87
DO  - 10.2298/JSC211214033B
SP  - 983
EP  - 995
ER  - 

@article{
author = "Bugeja Douglas, Adrian and Nešović, Milica and Šikoparija, Branko and Radišić, Predrag and Tosti, Tomislav and Trifković, Jelena and Russi, Luigi and Attard, Everaldo and Tešić, Živoslav and Gašić, Uroš",
year = "2022",
abstract = "Malta, a country renowned for its honey, has not been extensively mentioned in studies based on honey. In addition to many parameters, the collection period affects honey quality, precisely due to the different floral composition that exists during a certain season. Therefore, the significance of this study refers to the provision of data on honey from Malta collected during the autumn, spring, and summer seasons. Melissopalynological analysis, determination of physicochemical parameters, and the use of analytical chromatographic methods enabled detailed analysis of this honey. Principal component analysis (PCA) provided the differentiation of Maltese honey depending on the harvest season. Lotus pollen, followed by Eucalyptus, predominated in all honey samples. Characteristic compounds for summer honey were pinocembrin, galangin, kaempferol, chrysin, p-hydroxybenzoic acid, vanillic acid and maltotriose, while quercetin 3-O-galactoside, ferulic acid, ellagic acid, protocatechuic acid, luteolin 7-O-glucoside and melibiose were specific for autumn honey. A higher amount of p-coumaric acid, genistein, catechin, as well as the content of many sugars were found in spring samples. To the best of our knowledge, this is the first scientific work dealing with a detailed chemical analysis of Maltese honey.",
publisher = "Belgrade: Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Melissopalynology analysis, determination of physicochemical parameters, sugars and phenolics in Maltese honey collected in different seasons",
number = "9",
volume = "87",
doi = "10.2298/JSC211214033B",
pages = "983-995"
}

Bugeja Douglas, A., Nešović, M., Šikoparija, B., Radišić, P., Tosti, T., Trifković, J., Russi, L., Attard, E., Tešić, Ž.,& Gašić, U.. (2022). Melissopalynology analysis, determination of physicochemical parameters, sugars and phenolics in Maltese honey collected in different seasons. in Journal of the Serbian Chemical Society
Belgrade: Serbian Chemical Society., 87(9), 983-995.
https://doi.org/10.2298/JSC211214033B

Bugeja Douglas A, Nešović M, Šikoparija B, Radišić P, Tosti T, Trifković J, Russi L, Attard E, Tešić Ž, Gašić U. Melissopalynology analysis, determination of physicochemical parameters, sugars and phenolics in Maltese honey collected in different seasons. in Journal of the Serbian Chemical Society. 2022;87(9):983-995.
doi:10.2298/JSC211214033B .

Bugeja Douglas, Adrian, Nešović, Milica, Šikoparija, Branko, Radišić, Predrag, Tosti, Tomislav, Trifković, Jelena, Russi, Luigi, Attard, Everaldo, Tešić, Živoslav, Gašić, Uroš, "Melissopalynology analysis, determination of physicochemical parameters, sugars and phenolics in Maltese honey collected in different seasons" in Journal of the Serbian Chemical Society, 87, no. 9 (2022):983-995,
https://doi.org/10.2298/JSC211214033B . .

TY  - JOUR
AU  - Nešović, Milica
AU  - Gašić, Uroš
AU  - Tosti, Tomislav
AU  - Horvacki, Nikola
AU  - Šikoparija, Branko
AU  - Nedić, Nebojša
AU  - Blagojević, Stevan
AU  - Ignjatović, Ljubiša
AU  - Tešić, Živoslav
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4058
AB  - A focus of research in recent years is the comparison of honey as the final product of bees with pollen and nectar of the plant from which the honey originates, as the main food source for bees. Buckwheat honey is recognized as a nutritionally valuable product, which provides a scientifically proven health benefit and is confirmed as a functional food. The quality of this type of honey is attributed to high levels of phytochemicals in buckwheat. The purpose of this study was the examination of similarity between buckwheat honey and buckwheat nectar and pollen, as well as simultaneous investigation of their chemical profiles and the origin of the honey. The phenolic profile of buckwheat pollen showed a lower number of flavonoids and phenolic acids than those of nectar and honey samples, but confirmed the presence of the most characteristic polyphenols derived from the buckwheat plant. The notable difference was found to be the presence of (epi)catechin units, its galloylated derivatives and procyanidin dimers, which were not present in honey. Honey polyphenols displayed a pronounced correlation with those of nectar, but not with those of pollen. Finally, by comparing the polyphenolic profiles of honey, nectar and pollen sharing the same geographical origin, new data could be provided for a potential assessment of the botanical origin of buckwheat honey.
PB  - Royal Society of Chemistry
T2  - Royal Society Open Science
T1  - Polyphenolic profile of buckwheat honey, nectar and pollen
IS  - 12
VL  - 7
DO  - 10.1098/rsos.201576
SP  - 201576
ER  - 

@article{
author = "Nešović, Milica and Gašić, Uroš and Tosti, Tomislav and Horvacki, Nikola and Šikoparija, Branko and Nedić, Nebojša and Blagojević, Stevan and Ignjatović, Ljubiša and Tešić, Živoslav",
year = "2020",
abstract = "A focus of research in recent years is the comparison of honey as the final product of bees with pollen and nectar of the plant from which the honey originates, as the main food source for bees. Buckwheat honey is recognized as a nutritionally valuable product, which provides a scientifically proven health benefit and is confirmed as a functional food. The quality of this type of honey is attributed to high levels of phytochemicals in buckwheat. The purpose of this study was the examination of similarity between buckwheat honey and buckwheat nectar and pollen, as well as simultaneous investigation of their chemical profiles and the origin of the honey. The phenolic profile of buckwheat pollen showed a lower number of flavonoids and phenolic acids than those of nectar and honey samples, but confirmed the presence of the most characteristic polyphenols derived from the buckwheat plant. The notable difference was found to be the presence of (epi)catechin units, its galloylated derivatives and procyanidin dimers, which were not present in honey. Honey polyphenols displayed a pronounced correlation with those of nectar, but not with those of pollen. Finally, by comparing the polyphenolic profiles of honey, nectar and pollen sharing the same geographical origin, new data could be provided for a potential assessment of the botanical origin of buckwheat honey.",
publisher = "Royal Society of Chemistry",
journal = "Royal Society Open Science",
title = "Polyphenolic profile of buckwheat honey, nectar and pollen",
number = "12",
volume = "7",
doi = "10.1098/rsos.201576",
pages = "201576"
}

Nešović, M., Gašić, U., Tosti, T., Horvacki, N., Šikoparija, B., Nedić, N., Blagojević, S., Ignjatović, L.,& Tešić, Ž.. (2020). Polyphenolic profile of buckwheat honey, nectar and pollen. in Royal Society Open Science
Royal Society of Chemistry., 7(12), 201576.
https://doi.org/10.1098/rsos.201576

Nešović M, Gašić U, Tosti T, Horvacki N, Šikoparija B, Nedić N, Blagojević S, Ignjatović L, Tešić Ž. Polyphenolic profile of buckwheat honey, nectar and pollen. in Royal Society Open Science. 2020;7(12):201576.
doi:10.1098/rsos.201576 .

Nešović, Milica, Gašić, Uroš, Tosti, Tomislav, Horvacki, Nikola, Šikoparija, Branko, Nedić, Nebojša, Blagojević, Stevan, Ignjatović, Ljubiša, Tešić, Živoslav, "Polyphenolic profile of buckwheat honey, nectar and pollen" in Royal Society Open Science, 7, no. 12 (2020):201576,
https://doi.org/10.1098/rsos.201576 . .

TY  - JOUR
AU  - Mosić, Mirjana
AU  - Trifković, Jelena
AU  - Vovk, Irena
AU  - Gašić, Uroš
AU  - Tešić, Živoslav
AU  - Šikoparija, Branko
AU  - Milojković-Opsenica, Dušanka
PY  - 2019
UR  - https://www.mdpi.com/2218-273X/9/12/783
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3549
AB  - Information on compositional, nutritional and functional properties of bee-pollen, as a health-promoting food, is essential for defining its quality. Concerning the nutritional importance of phenolic compounds, the aim of this study was to determine the phenolic profile and antioxidant activity of twenty-four bee-pollen samples collected from different regions of Serbia. High-performance thin-layer chromatographic (HPTLC) fingerprinting was used for profiling of bee-pollen samples according to the botanical type. HPTLC hyphenated with image analysis and a pattern recognition technique confirmed the grouping of samples caused by the specific phenolic composition of pollens of different botanical origin. Flavonoid glycosides in bee-pollen samples were identified by applying ultra-high-performance liquid chromatography (UHPLC) coupled with linear ion trap-Orbitrap mass spectrometry (LTQ Orbitrap MS). Eight out of twenty-seven flavonol glycosides were identified in bee-pollen samples for the first time. All analyzed bee-pollen samples showed a high number of phenolic compounds which may have therapeutic potential.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Biomolecules
T2  - Biomolecules
T1  - Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen.
IS  - 12
VL  - 9
DO  - 10.3390/biom9120783
SP  - 783
ER  - 

@article{
author = "Mosić, Mirjana and Trifković, Jelena and Vovk, Irena and Gašić, Uroš and Tešić, Živoslav and Šikoparija, Branko and Milojković-Opsenica, Dušanka",
year = "2019",
abstract = "Information on compositional, nutritional and functional properties of bee-pollen, as a health-promoting food, is essential for defining its quality. Concerning the nutritional importance of phenolic compounds, the aim of this study was to determine the phenolic profile and antioxidant activity of twenty-four bee-pollen samples collected from different regions of Serbia. High-performance thin-layer chromatographic (HPTLC) fingerprinting was used for profiling of bee-pollen samples according to the botanical type. HPTLC hyphenated with image analysis and a pattern recognition technique confirmed the grouping of samples caused by the specific phenolic composition of pollens of different botanical origin. Flavonoid glycosides in bee-pollen samples were identified by applying ultra-high-performance liquid chromatography (UHPLC) coupled with linear ion trap-Orbitrap mass spectrometry (LTQ Orbitrap MS). Eight out of twenty-seven flavonol glycosides were identified in bee-pollen samples for the first time. All analyzed bee-pollen samples showed a high number of phenolic compounds which may have therapeutic potential.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Biomolecules, Biomolecules",
title = "Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen.",
number = "12",
volume = "9",
doi = "10.3390/biom9120783",
pages = "783"
}

Mosić, M., Trifković, J., Vovk, I., Gašić, U., Tešić, Ž., Šikoparija, B.,& Milojković-Opsenica, D.. (2019). Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen.. in Biomolecules
Multidisciplinary Digital Publishing Institute., 9(12), 783.
https://doi.org/10.3390/biom9120783

Mosić M, Trifković J, Vovk I, Gašić U, Tešić Ž, Šikoparija B, Milojković-Opsenica D. Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen.. in Biomolecules. 2019;9(12):783.
doi:10.3390/biom9120783 .

Mosić, Mirjana, Trifković, Jelena, Vovk, Irena, Gašić, Uroš, Tešić, Živoslav, Šikoparija, Branko, Milojković-Opsenica, Dušanka, "Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen." in Biomolecules, 9, no. 12 (2019):783,
https://doi.org/10.3390/biom9120783 . .

TY  - JOUR
AU  - Orčić, Snežana
AU  - Nikolić, Tatjana
AU  - Purać, Jelena
AU  - Šikoparija, Branko
AU  - Blagojević, Duško
AU  - Vukašinović, Elvira
AU  - Plavša, Nada
AU  - Stevanović, Jevrosima
AU  - Kojić, Danijela
PY  - 2017
UR  - http://doi.wiley.com/10.1111/eea.12633
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2964
AB  - The recent decline in managed honey bee populations, Apis mellifera L. (Hymenoptera: Apidae), has caused scientific, ecological, and economic concern. Research into the formation of reactive oxygen species (ROS), antioxidative defense mechanisms, and oxidative stress can contribute to our understanding of bee survival and conservation of this species. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) enzymes together with levels of malondialdehyde (MDA) were measured in summer and winter honey bees sampled from three colonies. One colony was stationary (C1), entering the winter period having accumulated Robinia pseudoacacia L. (Fabaceae) honey, and two were migratory (C2 and C3), entering the winter period with mainly Tilia (Malvaceae) and Brassica (Brassicaceae) honey, respectively. Compared to summer workers, winter worker bees had decreased SOD and GST activity, and MDA level, whereas CAT activity increased in all three colonies. We also demonstrated that seasonality is the main factor responsible for changes in antioxidant enzymes and MDA levels in worker honey bees. Overall, our results indicate a difference between summer and winter worker bees, pointing at a reduced level of antioxidant enzyme defenses during overwintering which may be due to a decrease in production of ROS. The decreased levels of MDA measured in winter honey bees confirm this. As ROS are actively used by insects as a defense mechanism to fight pathogens, we suggest that reduced production of ROS contributes to higher susceptibility of winter honey bees to infections and reduced overwinter survival.
T2  - Entomologia Experimentalis et Applicata
T1  - Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees
IS  - 2-3
VL  - 165
DO  - 10.1111/eea.12633
SP  - 120
EP  - 128
ER  - 

@article{
author = "Orčić, Snežana and Nikolić, Tatjana and Purać, Jelena and Šikoparija, Branko and Blagojević, Duško and Vukašinović, Elvira and Plavša, Nada and Stevanović, Jevrosima and Kojić, Danijela",
year = "2017",
abstract = "The recent decline in managed honey bee populations, Apis mellifera L. (Hymenoptera: Apidae), has caused scientific, ecological, and economic concern. Research into the formation of reactive oxygen species (ROS), antioxidative defense mechanisms, and oxidative stress can contribute to our understanding of bee survival and conservation of this species. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) enzymes together with levels of malondialdehyde (MDA) were measured in summer and winter honey bees sampled from three colonies. One colony was stationary (C1), entering the winter period having accumulated Robinia pseudoacacia L. (Fabaceae) honey, and two were migratory (C2 and C3), entering the winter period with mainly Tilia (Malvaceae) and Brassica (Brassicaceae) honey, respectively. Compared to summer workers, winter worker bees had decreased SOD and GST activity, and MDA level, whereas CAT activity increased in all three colonies. We also demonstrated that seasonality is the main factor responsible for changes in antioxidant enzymes and MDA levels in worker honey bees. Overall, our results indicate a difference between summer and winter worker bees, pointing at a reduced level of antioxidant enzyme defenses during overwintering which may be due to a decrease in production of ROS. The decreased levels of MDA measured in winter honey bees confirm this. As ROS are actively used by insects as a defense mechanism to fight pathogens, we suggest that reduced production of ROS contributes to higher susceptibility of winter honey bees to infections and reduced overwinter survival.",
journal = "Entomologia Experimentalis et Applicata",
title = "Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees",
number = "2-3",
volume = "165",
doi = "10.1111/eea.12633",
pages = "120-128"
}

Orčić, S., Nikolić, T., Purać, J., Šikoparija, B., Blagojević, D., Vukašinović, E., Plavša, N., Stevanović, J.,& Kojić, D.. (2017). Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees. in Entomologia Experimentalis et Applicata, 165(2-3), 120-128.
https://doi.org/10.1111/eea.12633

Orčić S, Nikolić T, Purać J, Šikoparija B, Blagojević D, Vukašinović E, Plavša N, Stevanović J, Kojić D. Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees. in Entomologia Experimentalis et Applicata. 2017;165(2-3):120-128.
doi:10.1111/eea.12633 .

Orčić, Snežana, Nikolić, Tatjana, Purać, Jelena, Šikoparija, Branko, Blagojević, Duško, Vukašinović, Elvira, Plavša, Nada, Stevanović, Jevrosima, Kojić, Danijela, "Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees" in Entomologia Experimentalis et Applicata, 165, no. 2-3 (2017):120-128,
https://doi.org/10.1111/eea.12633 . .