TY  - JOUR
AU  - Li, Hanluo
AU  - Masieri, Federica Francesca
AU  - Schneider, Marie
AU  - Bartella, Alexander
AU  - Gaus, Sebastian
AU  - Hahnel, Sebastian
AU  - Zimmerer, Rüdiger
AU  - Sack, Ulrich
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Simon, Jan-Christoph
AU  - Lethaus, Bernd
AU  - Savković, Vuk
PY  - 2021
UR  - internal-pdf://Li et al. - 2021 - The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem.pdf
UR  - https://www.mdpi.com/2218-273X/11/2/154
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4149
AB  - Hair follicle outer root sheath (ORS) is a putative source of stem cells with therapeutic capacity. ORS contains several multipotent stem cell populations, primarily in the distal compartment of the bulge region. However, the bulge is routinely obtained using invasive isolation methods, which require human scalp tissue ex vivo. Non-invasive sampling has been standardized by means of the plucking procedure, enabling to reproducibly obtain the mid-ORS part. The mid-ORS shows potential for giving rise to multiple stem cell populations in vitro. To demonstrate the phenotypic features of distal, middle, and proximal ORS parts, gene and protein expression profiles were studied in physically separated portions. The mid-part of the ORS showed a comparable or higher NGFR, nestin/NES, CD34, CD73, CD44, CD133, CK5, PAX3, MITF, and PMEL expression on both protein and gene levels, when compared to the distal ORS part. Distinct subpopulations of cells exhibiting small and round morphology were characterized with flow cytometry as simultaneously expressing CD73/CD271, CD49f/CD105, nestin, and not CK10. Potentially, these distinct subpopulations can give rise to cultured neuroectodermal and mesenchymal stem cell populations in vitro. In conclusion, the mid part of the ORS holds the potential for yielding multiple stem cells, in particular mesenchymal stem cells.
PB  - MDPI AG
T2  - Biomolecules
T1  - The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers
IS  - 2
VL  - 11
DO  - 10.3390/biom11020154
SP  - 154
ER  - 

@article{
author = "Li, Hanluo and Masieri, Federica Francesca and Schneider, Marie and Bartella, Alexander and Gaus, Sebastian and Hahnel, Sebastian and Zimmerer, Rüdiger and Sack, Ulrich and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Simon, Jan-Christoph and Lethaus, Bernd and Savković, Vuk",
year = "2021",
abstract = "Hair follicle outer root sheath (ORS) is a putative source of stem cells with therapeutic capacity. ORS contains several multipotent stem cell populations, primarily in the distal compartment of the bulge region. However, the bulge is routinely obtained using invasive isolation methods, which require human scalp tissue ex vivo. Non-invasive sampling has been standardized by means of the plucking procedure, enabling to reproducibly obtain the mid-ORS part. The mid-ORS shows potential for giving rise to multiple stem cell populations in vitro. To demonstrate the phenotypic features of distal, middle, and proximal ORS parts, gene and protein expression profiles were studied in physically separated portions. The mid-part of the ORS showed a comparable or higher NGFR, nestin/NES, CD34, CD73, CD44, CD133, CK5, PAX3, MITF, and PMEL expression on both protein and gene levels, when compared to the distal ORS part. Distinct subpopulations of cells exhibiting small and round morphology were characterized with flow cytometry as simultaneously expressing CD73/CD271, CD49f/CD105, nestin, and not CK10. Potentially, these distinct subpopulations can give rise to cultured neuroectodermal and mesenchymal stem cell populations in vitro. In conclusion, the mid part of the ORS holds the potential for yielding multiple stem cells, in particular mesenchymal stem cells.",
publisher = "MDPI AG",
journal = "Biomolecules",
title = "The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers",
number = "2",
volume = "11",
doi = "10.3390/biom11020154",
pages = "154"
}

Li, H., Masieri, F. F., Schneider, M., Bartella, A., Gaus, S., Hahnel, S., Zimmerer, R., Sack, U., Maksimović-Ivanić, D., Mijatović, S., Simon, J., Lethaus, B.,& Savković, V.. (2021). The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers. in Biomolecules
MDPI AG., 11(2), 154.
https://doi.org/10.3390/biom11020154

Li H, Masieri FF, Schneider M, Bartella A, Gaus S, Hahnel S, Zimmerer R, Sack U, Maksimović-Ivanić D, Mijatović S, Simon J, Lethaus B, Savković V. The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers. in Biomolecules. 2021;11(2):154.
doi:10.3390/biom11020154 .

Li, Hanluo, Masieri, Federica Francesca, Schneider, Marie, Bartella, Alexander, Gaus, Sebastian, Hahnel, Sebastian, Zimmerer, Rüdiger, Sack, Ulrich, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Simon, Jan-Christoph, Lethaus, Bernd, Savković, Vuk, "The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers" in Biomolecules, 11, no. 2 (2021):154,
https://doi.org/10.3390/biom11020154 . .