TY  - CONF
AU  - Marković Filipović, Jelena
AU  - Kojić, Danijela
AU  - Miler, Marko
AU  - Karan, Jelena
AU  - Ivelja, Ivana
AU  - Matavulj, Milica
PY  - 2022
UR  - https://www.hbd-sbc.hr/kongres2022/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6564
AB  - Acrylamide (AA) is carcinogen, mutagen and neurotoxic substance present in fried, roasted and
baked starch-based goods. AA is formed in Maillard reaction from asparagine and carbonyl
sources, such as reducing sugars, during thermal food processing at temperatures between
120°C and 180°C. The aim of our study was to determine whether acrylamide treatment affects
the expression and activity of glutathione-S-transferase (GST) in hepatocyte. Viability of rat
hepatoma cell line - H4IIE upon AA treatment was assessed by the MTT viability assay. Cells were
cultivated in a 96-well plate and exposed to increasing concentrations of AA (2.5–5.5 mM) for 24
h. AA concentration of 4 mM induced death of 17.62% H4IIE cells, while 4.5 mM AA caused death
of 48.45% cells. Therefore, AA concentrations of 4 mM and 4.5 mM were taken as IC20 and IC50,
respectively. H4IIE cells were treated with IC20 and IC50 of AA for 24 h. Relative mRNA
expression for GSTA2 and GSTP1 was quantified using real-time RT-PCR. Total GST activity was
determined spectrophotometrically at 340 nm using DTNB as the substrate. After AA treatments,
mRNA level for GSTA2 and GSTP1 and total GST activity increased in a concentration-dependent
manner in H4IIE cells. Only treatment with higher AA concentration (4.5 mM) proved to be
statistically significant for all three examined parameters. Our results indicate that by altering
expression and activity of GST enzyme acrylamide exerts toxic effects on hepatocytes.
PB  - Zagreb: Hrvatsko biološko društvo
C3  - Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska
T1  - Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes
SP  - 231
EP  - 232
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6564
ER  - 

@conference{
author = "Marković Filipović, Jelena and Kojić, Danijela and Miler, Marko and Karan, Jelena and Ivelja, Ivana and Matavulj, Milica",
year = "2022",
abstract = "Acrylamide (AA) is carcinogen, mutagen and neurotoxic substance present in fried, roasted and
baked starch-based goods. AA is formed in Maillard reaction from asparagine and carbonyl
sources, such as reducing sugars, during thermal food processing at temperatures between
120°C and 180°C. The aim of our study was to determine whether acrylamide treatment affects
the expression and activity of glutathione-S-transferase (GST) in hepatocyte. Viability of rat
hepatoma cell line - H4IIE upon AA treatment was assessed by the MTT viability assay. Cells were
cultivated in a 96-well plate and exposed to increasing concentrations of AA (2.5–5.5 mM) for 24
h. AA concentration of 4 mM induced death of 17.62% H4IIE cells, while 4.5 mM AA caused death
of 48.45% cells. Therefore, AA concentrations of 4 mM and 4.5 mM were taken as IC20 and IC50,
respectively. H4IIE cells were treated with IC20 and IC50 of AA for 24 h. Relative mRNA
expression for GSTA2 and GSTP1 was quantified using real-time RT-PCR. Total GST activity was
determined spectrophotometrically at 340 nm using DTNB as the substrate. After AA treatments,
mRNA level for GSTA2 and GSTP1 and total GST activity increased in a concentration-dependent
manner in H4IIE cells. Only treatment with higher AA concentration (4.5 mM) proved to be
statistically significant for all three examined parameters. Our results indicate that by altering
expression and activity of GST enzyme acrylamide exerts toxic effects on hepatocytes.",
publisher = "Zagreb: Hrvatsko biološko društvo",
journal = "Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska",
title = "Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes",
pages = "231-232",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6564"
}

Marković Filipović, J., Kojić, D., Miler, M., Karan, J., Ivelja, I.,& Matavulj, M.. (2022). Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes. in Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska
Zagreb: Hrvatsko biološko društvo., 231-232.
https://hdl.handle.net/21.15107/rcub_ibiss_6564

Marković Filipović J, Kojić D, Miler M, Karan J, Ivelja I, Matavulj M. Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes. in Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska. 2022;:231-232.
https://hdl.handle.net/21.15107/rcub_ibiss_6564 .

Marković Filipović, Jelena, Kojić, Danijela, Miler, Marko, Karan, Jelena, Ivelja, Ivana, Matavulj, Milica, "Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes" in Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska (2022):231-232,
https://hdl.handle.net/21.15107/rcub_ibiss_6564 .

TY  - JOUR
AU  - Marković Filipović, Jelena
AU  - Miler, Marko
AU  - Kojić, Danijela
AU  - Karan, Jelena
AU  - Ivelja, Ivana
AU  - Čukuranović Kokoris, Jovana
AU  - Matavulj, Milica
PY  - 2022
UR  - https://www.mdpi.com/1422-0067/23/11/6062
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5007
AB  - Acrylamide (AA) toxicity is associated with oxidative stress. During detoxification, AA is either coupled to gluthatione or biotransformed to glycidamide by the enzyme cytochrome P450 2E1 (CYP2E1). The aim of our study was to examine the hepatotoxicity of AA in vivo and in vitro. Thirty male Wistar rats were treated with 25 or 50 mg/kg b.w. of AA for 3 weeks. Qualitative and quantitative immunohistochemical evaluation of inducible nitric oxide synthase (iNOS), CYP2E1, catalase (CAT), superoxide dismutase 1 (SOD1), and SOD2 expression in liver was carried out. Bearing in mind that the liver is consisted mainly of hepatocytes, in a parallel study, we used the rat hepatoma cell line H4IIE to investigate the effects of AA at IC20 and IC50 concentrations on the redox status and the activity of CAT, SOD, and glutathione-S-transferase (GST), their gene expression, and CYP2E1 and iNOS expression. Immunohistochemically stained liver sections showed that treatment with AA25mg induced a significant decrease of CYP2E1 protein expression (p < 0.05), while treatment with AA50mg led to a significant increase of iNOS protein expression (p < 0.05). AA treatment dose-dependently elevated SOD2 protein expression (p < 0.05), while SOD1 protein expression was significantly increased only at AA50mg (p < 0.05). CAT protein expression was not significantly affected by AA treatments (p > 0.05). In AA-treated H4IIE cells, a concentration-dependent significant increase in lipid peroxidation and nitrite levels was observed (p < 0.05), while GSH content and SOD activity significantly decreased in a concentration-dependent manner (p < 0.05). AA IC50 significantly enhanced GST activity (p < 0.05). The level of mRNA significantly increased in a concentration-dependent manner for iNOS, SOD2, and CAT in AA-treated H4IIE cells (p < 0.05). AA IC50 significantly increased the transcription of SOD1, GSTA2, and GSTP1 genes (p < 0.05), while AA IC20 significantly decreased mRNA for CYP2E1 in H4IIE cells (p < 0.05). Obtained results indicate that AA treatments, both in vivo and in vitro, change hepatocytes; drug-metabolizing potential and disturb its redox status.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes.
IS  - 11
VL  - 23
DO  - 10.3390/ijms23116062
SP  - 6062
ER  - 

@article{
author = "Marković Filipović, Jelena and Miler, Marko and Kojić, Danijela and Karan, Jelena and Ivelja, Ivana and Čukuranović Kokoris, Jovana and Matavulj, Milica",
year = "2022",
abstract = "Acrylamide (AA) toxicity is associated with oxidative stress. During detoxification, AA is either coupled to gluthatione or biotransformed to glycidamide by the enzyme cytochrome P450 2E1 (CYP2E1). The aim of our study was to examine the hepatotoxicity of AA in vivo and in vitro. Thirty male Wistar rats were treated with 25 or 50 mg/kg b.w. of AA for 3 weeks. Qualitative and quantitative immunohistochemical evaluation of inducible nitric oxide synthase (iNOS), CYP2E1, catalase (CAT), superoxide dismutase 1 (SOD1), and SOD2 expression in liver was carried out. Bearing in mind that the liver is consisted mainly of hepatocytes, in a parallel study, we used the rat hepatoma cell line H4IIE to investigate the effects of AA at IC20 and IC50 concentrations on the redox status and the activity of CAT, SOD, and glutathione-S-transferase (GST), their gene expression, and CYP2E1 and iNOS expression. Immunohistochemically stained liver sections showed that treatment with AA25mg induced a significant decrease of CYP2E1 protein expression (p < 0.05), while treatment with AA50mg led to a significant increase of iNOS protein expression (p < 0.05). AA treatment dose-dependently elevated SOD2 protein expression (p < 0.05), while SOD1 protein expression was significantly increased only at AA50mg (p < 0.05). CAT protein expression was not significantly affected by AA treatments (p > 0.05). In AA-treated H4IIE cells, a concentration-dependent significant increase in lipid peroxidation and nitrite levels was observed (p < 0.05), while GSH content and SOD activity significantly decreased in a concentration-dependent manner (p < 0.05). AA IC50 significantly enhanced GST activity (p < 0.05). The level of mRNA significantly increased in a concentration-dependent manner for iNOS, SOD2, and CAT in AA-treated H4IIE cells (p < 0.05). AA IC50 significantly increased the transcription of SOD1, GSTA2, and GSTP1 genes (p < 0.05), while AA IC20 significantly decreased mRNA for CYP2E1 in H4IIE cells (p < 0.05). Obtained results indicate that AA treatments, both in vivo and in vitro, change hepatocytes; drug-metabolizing potential and disturb its redox status.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes.",
number = "11",
volume = "23",
doi = "10.3390/ijms23116062",
pages = "6062"
}

Marković Filipović, J., Miler, M., Kojić, D., Karan, J., Ivelja, I., Čukuranović Kokoris, J.,& Matavulj, M.. (2022). Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes.. in International Journal of Molecular Sciences
Basel: MDPI., 23(11), 6062.
https://doi.org/10.3390/ijms23116062

Marković Filipović J, Miler M, Kojić D, Karan J, Ivelja I, Čukuranović Kokoris J, Matavulj M. Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes.. in International Journal of Molecular Sciences. 2022;23(11):6062.
doi:10.3390/ijms23116062 .

Marković Filipović, Jelena, Miler, Marko, Kojić, Danijela, Karan, Jelena, Ivelja, Ivana, Čukuranović Kokoris, Jovana, Matavulj, Milica, "Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes." in International Journal of Molecular Sciences, 23, no. 11 (2022):6062,
https://doi.org/10.3390/ijms23116062 . .

TY  - CONF
AU  - Marković Filipović, Jelena
AU  - Ivelja, Ivana
AU  - Karan, Jelena
AU  - Miler, Marko
AU  - Matavulj, Milica
PY  - 2022
UR  - https://www.serbiosoc.org.rs/?page_id=702
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6036
AB  - Akrilamid (AA) je toksična industrijska hemikalija čiji se metabolizam odvija u jetri. Dodatno, AA se nalazi u često konzumiranoj hrani bogatoj ugljenim hidratima koja se sprema na visokim temperaturama. Cilj naše studije je da ispitamo efekat AA na proliferativne i apoptotičke karakteristike jetre odraslih pacova, koristeći imunohistohemijske (IHC) metode. Odrasli mužjaci pacova soja Wistar, stari 65 dana tretirani su dozama AA od 25 mg/kg i 50 mg/kg. Tkivo prethodno fiksirano u formalinu i ukalupljeno u parafin, isečeno je na preseke debljine 5 µm i obojeno imunohistohemijski koristeći anti-PCNA i anticaspase 3 antitela. Procenat obojene površine imunohistohemijski obojenih preseka određen je pomoću ImageJ dodatka – IHC profiler. U kontrolnoj grupi i grupama tretiranim akrilamidom ekspresija PCNA primećena je u jedrima hepatocita. AA tretman dovodi do povećanja brojnosti PCNA pozitivnih ćelija koje je zavisno od doze. Povećanje brojnosti PCNA pozitivnih ćelija pokazalo je i statističku značajnost u grupi tretiranoj sa AA50mg. U svim grupama, imunobojenje kaspazom 3 pokazalo je slabu citoplazmatsku imunoreaktivnost. Statistička analiza ukazala je na to da AA tretman nije značajno uticao na procenat ćelija pozitivnih na kaspazu 3. Imunohistohemijska analiza preseka jetre pokazala je da AA50mg tretman utiče na proliferativnost, ali ne i apoptozu hepatocita pacova.
AB  - Акриламид (АА) је токсична индустријска хемикалија чији се метаболизам одвија
у јетри. Додатно, АА се налази у често конзумираној храни богатој угљеним
хидратима која се спрема на високим температурама. Циљ наше студије је да
испитамо ефекат АА на пролиферативне и апоптотичке карактеристике јетре
одраслих пацова, користећи имунохистохемијске (ИХЦ) методе. Одрасли мужјаци
пацова соја Wistar, стари 65 дана третирани су дозама АА од 25 мг/кг и 50 мг/кг.
Ткиво претходно фиксирано у формалину и укалупљено у парафин, исечено је на
пресеке дебљине 5 µm и обојено имунохистохемијски користећи анти-PCNA и anticaspase 3 антитела. Проценат обојене површине имунохистохемијски обојених
пресека одређен је помоћу ImageJ додатка – IHC profiler. У контролној групи и
групама третираним акриламидом експресија PCNA примећена је у једрима
хепатоцита. АА третман доводи до повећања бројности PCNA позитивних ћелија
које је зависно од дозе. Повећање бројности PCNA позитивних ћелија показало је и
статистичку значајност у групи третираној са AA50мг. У свим групама, имунобојење
каспазом 3 показало је слабу цитоплазматску имунореактивност. Статистичка
анализа указала је на то да АА третман није значајно утицао на проценат ћелија
позитивних на каспазу 3. Имунохистохемијска анализа пресека јетре показала је да
AA50мг третман утиче на пролиферативност, али не и апоптозу хепатоцита пацова.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova
T1  - Ефекат третмана акриламидом на пролиферативне и апоптотичке карактеристике јетре одраслих пацова
SP  - 366
EP  - 366
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6036
ER  - 

@conference{
author = "Marković Filipović, Jelena and Ivelja, Ivana and Karan, Jelena and Miler, Marko and Matavulj, Milica",
year = "2022",
abstract = "Akrilamid (AA) je toksična industrijska hemikalija čiji se metabolizam odvija u jetri. Dodatno, AA se nalazi u često konzumiranoj hrani bogatoj ugljenim hidratima koja se sprema na visokim temperaturama. Cilj naše studije je da ispitamo efekat AA na proliferativne i apoptotičke karakteristike jetre odraslih pacova, koristeći imunohistohemijske (IHC) metode. Odrasli mužjaci pacova soja Wistar, stari 65 dana tretirani su dozama AA od 25 mg/kg i 50 mg/kg. Tkivo prethodno fiksirano u formalinu i ukalupljeno u parafin, isečeno je na preseke debljine 5 µm i obojeno imunohistohemijski koristeći anti-PCNA i anticaspase 3 antitela. Procenat obojene površine imunohistohemijski obojenih preseka određen je pomoću ImageJ dodatka – IHC profiler. U kontrolnoj grupi i grupama tretiranim akrilamidom ekspresija PCNA primećena je u jedrima hepatocita. AA tretman dovodi do povećanja brojnosti PCNA pozitivnih ćelija koje je zavisno od doze. Povećanje brojnosti PCNA pozitivnih ćelija pokazalo je i statističku značajnost u grupi tretiranoj sa AA50mg. U svim grupama, imunobojenje kaspazom 3 pokazalo je slabu citoplazmatsku imunoreaktivnost. Statistička analiza ukazala je na to da AA tretman nije značajno uticao na procenat ćelija pozitivnih na kaspazu 3. Imunohistohemijska analiza preseka jetre pokazala je da AA50mg tretman utiče na proliferativnost, ali ne i apoptozu hepatocita pacova., Акриламид (АА) је токсична индустријска хемикалија чији се метаболизам одвија
у јетри. Додатно, АА се налази у често конзумираној храни богатој угљеним
хидратима која се спрема на високим температурама. Циљ наше студије је да
испитамо ефекат АА на пролиферативне и апоптотичке карактеристике јетре
одраслих пацова, користећи имунохистохемијске (ИХЦ) методе. Одрасли мужјаци
пацова соја Wistar, стари 65 дана третирани су дозама АА од 25 мг/кг и 50 мг/кг.
Ткиво претходно фиксирано у формалину и укалупљено у парафин, исечено је на
пресеке дебљине 5 µm и обојено имунохистохемијски користећи анти-PCNA и anticaspase 3 антитела. Проценат обојене површине имунохистохемијски обојених
пресека одређен је помоћу ImageJ додатка – IHC profiler. У контролној групи и
групама третираним акриламидом експресија PCNA примећена је у једрима
хепатоцита. АА третман доводи до повећања бројности PCNA позитивних ћелија
које је зависно од дозе. Повећање бројности PCNA позитивних ћелија показало је и
статистичку значајност у групи третираној са AA50мг. У свим групама, имунобојење
каспазом 3 показало је слабу цитоплазматску имунореактивност. Статистичка
анализа указала је на то да АА третман није значајно утицао на проценат ћелија
позитивних на каспазу 3. Имунохистохемијска анализа пресека јетре показала је да
AA50мг третман утиче на пролиферативност, али не и апоптозу хепатоцита пацова.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova, Ефекат третмана акриламидом на пролиферативне и апоптотичке карактеристике јетре одраслих пацова",
pages = "366-366",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6036"
}

Marković Filipović, J., Ivelja, I., Karan, J., Miler, M.,& Matavulj, M.. (2022). Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 366-366.
https://hdl.handle.net/21.15107/rcub_ibiss_6036

Marković Filipović J, Ivelja I, Karan J, Miler M, Matavulj M. Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:366-366.
https://hdl.handle.net/21.15107/rcub_ibiss_6036 .

Marković Filipović, Jelena, Ivelja, Ivana, Karan, Jelena, Miler, Marko, Matavulj, Milica, "Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):366-366,
https://hdl.handle.net/21.15107/rcub_ibiss_6036 .

TY  - CONF
AU  - Marković Filipović, Jelena
AU  - Ivelja, Ivana
AU  - Karan, Jelena
AU  - Miler, Marko
AU  - Milošević, Verica
AU  - Matavulj, Milica
PY  - 2021
UR  - https://ibsc2021.pmf.uns.ac.rs/
UR  - https://ibsc2021.pmf.uns.ac.rs/ebook-of-abstracts/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6577
AB  - INTRODUCTION: Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory purposes. In addition, AA is formed in many types of fried and oven-baked foods during cooking. Considering proven neurotoxic, carcinogenic and mutagenic effects on living organisms, AA became a main topic of interest for many research groups. OBJECTIVES: The objective of our study was to determine whether acrylamide treatment affects superoxide dismutase 1 (SOD1) expression in rat liver. METHOD / DESIGN: Adult male Wistar rats were subchronicly (three weeks) treated with 25 mg/kg or 50 mg/kg body weight (b.w.) of AA. Formalin-fixed paraffin-embedded liver tissue was cut into 5 µm thin sections and immunostained with anti-SOD1 antibody. The amount of SOD1 in immunostained sections was determined using Windows based ImageJ program (ImageJ, Version 1.50f). The optical density (OD) and stained percentage color area of immunolabeled SOD1 were measured. RESULTS: Figure 1. Representative micrographs of superoxide dismutase 1 (SOD1) immunohistochemical staining in liver of control rats, rats treated with
acrylamide (AA) in dose of 25 mg/kg b.w., and rats treated with acrylamide in dose of 50 mg/kg b.w. (a). Optical density of SOD1 immunopositive cells in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (b). Percentage contribution of high positive, positive, low positive and total positive immunohistochemical staining of SOD1 in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (c). Values in charts are means ± SEM; n = 10, *p < 0.05. In statistical analysis AA-treated animals were compared with the control group. CONCLUSIONS: Immunostaining of SOD1 in liver of control rats showed weak cytoplasmic immunoreactivity in hepatocytes (Fig. 1a). AA application induced dose dependent increase of immunostaining intensity (Fig. 1a). Significant increase of OD and percentage contribution of low positive and total positive cells of immunostained SOD1 was detected in group treated with AA in a dose of 50 mg/kg (Fig.1b, c).
PB  - Novi Sad: Faculty of Sciences, University of Novi Sad
C3  - Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
T1  - Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver
SP  - 137
EP  - 138
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6577
ER  - 

@conference{
author = "Marković Filipović, Jelena and Ivelja, Ivana and Karan, Jelena and Miler, Marko and Milošević, Verica and Matavulj, Milica",
year = "2021",
abstract = "INTRODUCTION: Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory purposes. In addition, AA is formed in many types of fried and oven-baked foods during cooking. Considering proven neurotoxic, carcinogenic and mutagenic effects on living organisms, AA became a main topic of interest for many research groups. OBJECTIVES: The objective of our study was to determine whether acrylamide treatment affects superoxide dismutase 1 (SOD1) expression in rat liver. METHOD / DESIGN: Adult male Wistar rats were subchronicly (three weeks) treated with 25 mg/kg or 50 mg/kg body weight (b.w.) of AA. Formalin-fixed paraffin-embedded liver tissue was cut into 5 µm thin sections and immunostained with anti-SOD1 antibody. The amount of SOD1 in immunostained sections was determined using Windows based ImageJ program (ImageJ, Version 1.50f). The optical density (OD) and stained percentage color area of immunolabeled SOD1 were measured. RESULTS: Figure 1. Representative micrographs of superoxide dismutase 1 (SOD1) immunohistochemical staining in liver of control rats, rats treated with
acrylamide (AA) in dose of 25 mg/kg b.w., and rats treated with acrylamide in dose of 50 mg/kg b.w. (a). Optical density of SOD1 immunopositive cells in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (b). Percentage contribution of high positive, positive, low positive and total positive immunohistochemical staining of SOD1 in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (c). Values in charts are means ± SEM; n = 10, *p < 0.05. In statistical analysis AA-treated animals were compared with the control group. CONCLUSIONS: Immunostaining of SOD1 in liver of control rats showed weak cytoplasmic immunoreactivity in hepatocytes (Fig. 1a). AA application induced dose dependent increase of immunostaining intensity (Fig. 1a). Significant increase of OD and percentage contribution of low positive and total positive cells of immunostained SOD1 was detected in group treated with AA in a dose of 50 mg/kg (Fig.1b, c).",
publisher = "Novi Sad: Faculty of Sciences, University of Novi Sad",
journal = "Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia",
title = "Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver",
pages = "137-138",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6577"
}

Marković Filipović, J., Ivelja, I., Karan, J., Miler, M., Milošević, V.,& Matavulj, M.. (2021). Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
Novi Sad: Faculty of Sciences, University of Novi Sad., 137-138.
https://hdl.handle.net/21.15107/rcub_ibiss_6577

Marković Filipović J, Ivelja I, Karan J, Miler M, Milošević V, Matavulj M. Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia. 2021;:137-138.
https://hdl.handle.net/21.15107/rcub_ibiss_6577 .

Marković Filipović, Jelena, Ivelja, Ivana, Karan, Jelena, Miler, Marko, Milošević, Verica, Matavulj, Milica, "Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver" in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia (2021):137-138,
https://hdl.handle.net/21.15107/rcub_ibiss_6577 .