TY  - CONF
AU  - Ajdžanović, Vladimir
AU  - Čukuranović-Kokoris, Jovana
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka
AU  - Živanović, Jasmina
AU  - Marković Filipović, Jelena
AU  - Milošević, Verica
AU  - Filipović, Branko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6405
AB  - Uvod. Aplikacija daidzeina može predstavljati efikasnu i manje rizičnu altemativu indikovanoj, klasičnoj estrogenizaciji starijih muškaraca. Cilj ove studije je podrazumevao morfofiziološku analizu kore nadbubrežne žlezde u pacovskom modelu uznapredovale andropauze, nakon primene daidzeina.
Materijal i metode. Sredovečni (16 meseci stari) pacovi Wistar soja su razdeljeni u
lažno operisanu (SO; n=8), orhidektomisanu (Orx; n=8) i daidzeinom tretiranu orhidektomisanu (Orx+D; n=8) grupu. Daidzein (30 mg/kg t.m./dan) je subkutano davan tri nedelje, dok su SO i Orx grupa dobijale samo rastvarač tokom istog perioda. Ciljevi su realizovani korišćenjem stereologije, histohemije/imunohistohemije, imunoeseja i ultrastruktume analize.
Rezultati. Tretman daidzeinom je značajno uvećao volumene ćelija i jedara zone glomeruloze 
za 32% i 41% tim redom, ali je i snizio (p<0.05) nivo cirkulišućeg aldosterona za 61%, sve u 
poređenju sa odgovarajućim parametrima kod Orx zivotinja. Takođe, daidzein je značajno smanjio 
volumen jedara ćelija zone fascikulate, kao i tkivni i cirkulišući nivo kortikosterona, za 24%, 48% i 33% 
navedenim redom, te izrazito (p<0.05) povisio cirkulišući nivo DHEA za oko 4.6 puta, sve u 
poređenju sa odgovarajućim parametrima kod Orx pacova. Daidzeinom prouzrokovano preusmeravanje 
puteva kortikosteroidogeneze ka produkciji adrenalnih androgena bilo je u skladu sa ultrastruktumim nalazom i rearanžmanima kapilarne rnreže u okviru konkretnih zona kore nadbubrežne zlezde.
Zaključak. Imajući u vidu sklad efekata daidzeina u ispitivanom kontekstu, kao relativnu bezbednost njegove upotrebe, moglo bi se reći da daidzein ima perspektivu u tretrnanu starenjem uzorokovanog gubitka androgena, te hiperfunkcije hipotalamo-hipofizno-adrenalne ose i povezanih metaboličkih poremećaja kod muškaraca.
AB  - Introduction. Daidzein application may represent effective and less harmful alternative to indicated, classical estrogenization of the aging men. The aim of this study was to perform morphophysiological analysis of the adrenal cortex, after daidzein supplementation in a rat model of the severe andropause.
Material and methods. Middle-aged (16 months old) Wistar rats were divided into sham operated (SO; n=8), orchidectomized (Orx; n=8) and daidzein treated orchidectomized (Orx+D; n=8) groups. Daidzein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using stereology, histochemistry/immunohistochemistry, immunoassays and ultrastructural analysis.
Results. Daidzein treatment significantly increased volumes of the zona glomerulosa cell and nuclei by 32% and 41% respectively, but decreased (p<0.05) circulating aldosterone levels by 61%, all in comparison with the adequate parameters in Orx animals. Also, daidzein significantly decreased zona fasciculata cell nuclei volume, as well as both the adrenal and circulating levels of corticosterone, by 24%, 48% and 33% respectively, but strongly (p<0.05) increased the circulating level of DHEA by about 4.6 times, all when compared to the corresponding parameters of Orx rats. Daidzein-induced shunting of the corticosteroidogenesis pathways towards adrenal androgens production corresponded to the ultrastructural findings and zonal capillary network rearrangements.
Conclusion. Given the coherence of its effects and relative safety, daidzein could be the remedy of choice for the treatment of aging-caused androgen deprivation and the hypothalamo-pituitary-adrenal axis hyperfunction/related metabolic issues in males.
PB  - Niš: University of Niš, Faculty of Medicine
C3  - Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
T1  - Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze
T1  - Morphophysiology of the adrenal cortex after daidzein application in a rat model of the severe andropause
SP  - 166
EP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6405
ER  - 

@conference{
author = "Ajdžanović, Vladimir and Čukuranović-Kokoris, Jovana and Miler, Marko and Šošić-Jurjević, Branka and Živanović, Jasmina and Marković Filipović, Jelena and Milošević, Verica and Filipović, Branko",
year = "2023",
abstract = "Uvod. Aplikacija daidzeina može predstavljati efikasnu i manje rizičnu altemativu indikovanoj, klasičnoj estrogenizaciji starijih muškaraca. Cilj ove studije je podrazumevao morfofiziološku analizu kore nadbubrežne žlezde u pacovskom modelu uznapredovale andropauze, nakon primene daidzeina.
Materijal i metode. Sredovečni (16 meseci stari) pacovi Wistar soja su razdeljeni u
lažno operisanu (SO; n=8), orhidektomisanu (Orx; n=8) i daidzeinom tretiranu orhidektomisanu (Orx+D; n=8) grupu. Daidzein (30 mg/kg t.m./dan) je subkutano davan tri nedelje, dok su SO i Orx grupa dobijale samo rastvarač tokom istog perioda. Ciljevi su realizovani korišćenjem stereologije, histohemije/imunohistohemije, imunoeseja i ultrastruktume analize.
Rezultati. Tretman daidzeinom je značajno uvećao volumene ćelija i jedara zone glomeruloze 
za 32% i 41% tim redom, ali je i snizio (p<0.05) nivo cirkulišućeg aldosterona za 61%, sve u 
poređenju sa odgovarajućim parametrima kod Orx zivotinja. Takođe, daidzein je značajno smanjio 
volumen jedara ćelija zone fascikulate, kao i tkivni i cirkulišući nivo kortikosterona, za 24%, 48% i 33% 
navedenim redom, te izrazito (p<0.05) povisio cirkulišući nivo DHEA za oko 4.6 puta, sve u 
poređenju sa odgovarajućim parametrima kod Orx pacova. Daidzeinom prouzrokovano preusmeravanje 
puteva kortikosteroidogeneze ka produkciji adrenalnih androgena bilo je u skladu sa ultrastruktumim nalazom i rearanžmanima kapilarne rnreže u okviru konkretnih zona kore nadbubrežne zlezde.
Zaključak. Imajući u vidu sklad efekata daidzeina u ispitivanom kontekstu, kao relativnu bezbednost njegove upotrebe, moglo bi se reći da daidzein ima perspektivu u tretrnanu starenjem uzorokovanog gubitka androgena, te hiperfunkcije hipotalamo-hipofizno-adrenalne ose i povezanih metaboličkih poremećaja kod muškaraca., Introduction. Daidzein application may represent effective and less harmful alternative to indicated, classical estrogenization of the aging men. The aim of this study was to perform morphophysiological analysis of the adrenal cortex, after daidzein supplementation in a rat model of the severe andropause.
Material and methods. Middle-aged (16 months old) Wistar rats were divided into sham operated (SO; n=8), orchidectomized (Orx; n=8) and daidzein treated orchidectomized (Orx+D; n=8) groups. Daidzein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using stereology, histochemistry/immunohistochemistry, immunoassays and ultrastructural analysis.
Results. Daidzein treatment significantly increased volumes of the zona glomerulosa cell and nuclei by 32% and 41% respectively, but decreased (p<0.05) circulating aldosterone levels by 61%, all in comparison with the adequate parameters in Orx animals. Also, daidzein significantly decreased zona fasciculata cell nuclei volume, as well as both the adrenal and circulating levels of corticosterone, by 24%, 48% and 33% respectively, but strongly (p<0.05) increased the circulating level of DHEA by about 4.6 times, all when compared to the corresponding parameters of Orx rats. Daidzein-induced shunting of the corticosteroidogenesis pathways towards adrenal androgens production corresponded to the ultrastructural findings and zonal capillary network rearrangements.
Conclusion. Given the coherence of its effects and relative safety, daidzein could be the remedy of choice for the treatment of aging-caused androgen deprivation and the hypothalamo-pituitary-adrenal axis hyperfunction/related metabolic issues in males.",
publisher = "Niš: University of Niš, Faculty of Medicine",
journal = "Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia",
title = "Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze, Morphophysiology of the adrenal cortex after daidzein application in a rat model of the severe andropause",
pages = "166-167",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6405"
}

Ajdžanović, V., Čukuranović-Kokoris, J., Miler, M., Šošić-Jurjević, B., Živanović, J., Marković Filipović, J., Milošević, V.,& Filipović, B.. (2023). Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
Niš: University of Niš, Faculty of Medicine., 166-167.
https://hdl.handle.net/21.15107/rcub_ibiss_6405

Ajdžanović V, Čukuranović-Kokoris J, Miler M, Šošić-Jurjević B, Živanović J, Marković Filipović J, Milošević V, Filipović B. Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia. 2023;:166-167.
https://hdl.handle.net/21.15107/rcub_ibiss_6405 .

Ajdžanović, Vladimir, Čukuranović-Kokoris, Jovana, Miler, Marko, Šošić-Jurjević, Branka, Živanović, Jasmina, Marković Filipović, Jelena, Milošević, Verica, Filipović, Branko, "Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze" in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia (2023):166-167,
https://hdl.handle.net/21.15107/rcub_ibiss_6405 .

TY  - CONF
AU  - Čukuranović-Kokoris, Jovana
AU  - Dovenska, Monika
AU  - Ajdžanović, Vladimir
AU  - Marković Filipović, Jelena
AU  - Ugrenović, Slađana
AU  - Kundalić, Braca
AU  - Milošević, Verica
AU  - Popovska-Perčinić, Florina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6278
AB  - Uvod. Faktori sredine, kao što je visoka ili niska temperatura ambijenta, predstavljaju snažne stresore za organizam. Hipofizne ćelije koje luče hormone, posebno somatotropne (GH), intenzivno reaguju na ove faktore. Cilj ove studije bio je da ispita efekat dugotrajnog (60-dnevnog) izlaganja pacova umereno visokoj temperaturi ambijenta (35±1°C) na imunohistomorfometrijske parametre GH ćelija hipofize.
Metode. Eksperiment je izveden na odraslim Wistar pacovima, podeljenim u dve jednake grupe: sedam pacova je kontinuirano bilo izloženo temperaturi 35± l°C tokom 60 dana (eksperimentalna grupa), dok je sedam pacova tokom istog perioda čuvano na 20±2°C (kontrolna grupa). Sve su životinje čuvane u režimu od 12 sati dnevne svetlosti i 12 sati mraka, dok su hranu i vodu mogle da uzimaju ad libitum. GH ćelije vizuelizovane su pomoću imunohistohemijske procedure peroksidaze-antiperoksidaze (PAP). Morfometrijska analiza izvedena je korišćenjem višenamenskog M42 test-sistema.
Rezultati. Nakon 60 dana izlaganja povišenoj ambijentalnoj temperaturi, telesna masa životinja bila je značajno smanjena za 16,4%, u poređenju sa kontrolama. GH ćelije u kontrolnoj i eksperimentalnoj grupi bile su ovalnog oblika i locirane u blizini kapilara. Tamne granule, distribuirane po citoplazmi GH ćelija, bile su sebi svojstvenih univer­zalnih karakteristika u obe grupe. Kod eksperimentalnih pacova, celularni i nukleami volumeni GH ćelija, kao i volumenska gustina GH ćelija, značajno su redukovani za 19,1%, 10,6% i 15,8%, redom, u poređenju sa intaktnim kontrolama.
Zaključak. Izlaganje odraslih pacova muškog pola umereno visokoj ambijentalnoj temperaturi u trajanju od 60 dana dovelo je do smanjenja histomorfometrijskih parametara GH ćelija.
PB  - Niš: University of Niš, Faculty of Medicine
C3  - Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
T1  - Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize
SP  - 160
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6278
ER  - 

@conference{
author = "Čukuranović-Kokoris, Jovana and Dovenska, Monika and Ajdžanović, Vladimir and Marković Filipović, Jelena and Ugrenović, Slađana and Kundalić, Braca and Milošević, Verica and Popovska-Perčinić, Florina",
year = "2023",
abstract = "Uvod. Faktori sredine, kao što je visoka ili niska temperatura ambijenta, predstavljaju snažne stresore za organizam. Hipofizne ćelije koje luče hormone, posebno somatotropne (GH), intenzivno reaguju na ove faktore. Cilj ove studije bio je da ispita efekat dugotrajnog (60-dnevnog) izlaganja pacova umereno visokoj temperaturi ambijenta (35±1°C) na imunohistomorfometrijske parametre GH ćelija hipofize.
Metode. Eksperiment je izveden na odraslim Wistar pacovima, podeljenim u dve jednake grupe: sedam pacova je kontinuirano bilo izloženo temperaturi 35± l°C tokom 60 dana (eksperimentalna grupa), dok je sedam pacova tokom istog perioda čuvano na 20±2°C (kontrolna grupa). Sve su životinje čuvane u režimu od 12 sati dnevne svetlosti i 12 sati mraka, dok su hranu i vodu mogle da uzimaju ad libitum. GH ćelije vizuelizovane su pomoću imunohistohemijske procedure peroksidaze-antiperoksidaze (PAP). Morfometrijska analiza izvedena je korišćenjem višenamenskog M42 test-sistema.
Rezultati. Nakon 60 dana izlaganja povišenoj ambijentalnoj temperaturi, telesna masa životinja bila je značajno smanjena za 16,4%, u poređenju sa kontrolama. GH ćelije u kontrolnoj i eksperimentalnoj grupi bile su ovalnog oblika i locirane u blizini kapilara. Tamne granule, distribuirane po citoplazmi GH ćelija, bile su sebi svojstvenih univer­zalnih karakteristika u obe grupe. Kod eksperimentalnih pacova, celularni i nukleami volumeni GH ćelija, kao i volumenska gustina GH ćelija, značajno su redukovani za 19,1%, 10,6% i 15,8%, redom, u poređenju sa intaktnim kontrolama.
Zaključak. Izlaganje odraslih pacova muškog pola umereno visokoj ambijentalnoj temperaturi u trajanju od 60 dana dovelo je do smanjenja histomorfometrijskih parametara GH ćelija.",
publisher = "Niš: University of Niš, Faculty of Medicine",
journal = "Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia",
title = "Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize",
pages = "160",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6278"
}

Čukuranović-Kokoris, J., Dovenska, M., Ajdžanović, V., Marković Filipović, J., Ugrenović, S., Kundalić, B., Milošević, V.,& Popovska-Perčinić, F.. (2023). Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
Niš: University of Niš, Faculty of Medicine., 160.
https://hdl.handle.net/21.15107/rcub_ibiss_6278

Čukuranović-Kokoris J, Dovenska M, Ajdžanović V, Marković Filipović J, Ugrenović S, Kundalić B, Milošević V, Popovska-Perčinić F. Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia. 2023;:160.
https://hdl.handle.net/21.15107/rcub_ibiss_6278 .

Čukuranović-Kokoris, Jovana, Dovenska, Monika, Ajdžanović, Vladimir, Marković Filipović, Jelena, Ugrenović, Slađana, Kundalić, Braca, Milošević, Verica, Popovska-Perčinić, Florina, "Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize" in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia (2023):160,
https://hdl.handle.net/21.15107/rcub_ibiss_6278 .

TY  - CONF
AU  - Čukuranović-Kokoris, Jovana
AU  - Đorđević, Miodrag
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Marković Filipović, Jelena
AU  - Stanković Đorević, Dobrila
AU  - Čukuranović, Rade
AU  - Milošević, Verica
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6229
AB  - Soy isoflavone daidzein exerts some beneficial effects in different ageing-related, chronic diseases and may represent a promising alternative to classical estrogenization of the prostate cancer suffering subjects. The aim of this study was to examine the effects of daidzein on the adrenocortical vascular network and hormonal output in a rat model of the mild andropause. Adult (3 months old) Wistar rats were divided into sham-operated (SO; n=7), orchidectomized (Orx; n=7) and daidzein treated orchidectomized (Orx+D; n=7) groups. Daidzein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Adrenocortical vascular network properties were identified using Novelli histochemical staining, vascular endothelial growth factor (VEGF) immunostaining and the newCast stereological software. Blood concentrations of aldosterone, corticosterone and DHEAS were measured by immunoassays. Volume densities of blood vessels and VEGF depots within the adrenal cortex were not significantly changed after daidzein treatment, although there was a tendency of decrease of these parameters. On the other hand, daidzein application increased (P<0.05) blood concentration of aldosterone by more than 2.5 times and 41%, in comparison with the same parameter in sham-operated and orchidectomized group, respectively. Corticosterone concentration in daidzein-treated, orchidectomized rats showed a tendency of elevation (P>0.05) compared to the both SO animals and orchidectomy alone. DHEAS blood concentration after daidzein application remained at the level of the Orx group, which in both cases ment elevation (P<0.05) by around 80% in comparison with the same parameter in SO group. We conclude that daidzein significantly increases aldosterone and to some lower extent corticosterone output in a rat model of the mild andropause, without significant rearrangements of the entire adrenal cortex vascular network.
PB  - bioscientifica
C3  - 25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey
T1  - Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause
DO  - 10.1530/endoabs.90.EP63
SP  - 394
EP  - 395
ER  - 

@conference{
author = "Čukuranović-Kokoris, Jovana and Đorđević, Miodrag and Miler, Marko and Živanović, Jasmina and Marković Filipović, Jelena and Stanković Đorević, Dobrila and Čukuranović, Rade and Milošević, Verica and Filipović, Branko and Ajdžanović, Vladimir",
year = "2023",
abstract = "Soy isoflavone daidzein exerts some beneficial effects in different ageing-related, chronic diseases and may represent a promising alternative to classical estrogenization of the prostate cancer suffering subjects. The aim of this study was to examine the effects of daidzein on the adrenocortical vascular network and hormonal output in a rat model of the mild andropause. Adult (3 months old) Wistar rats were divided into sham-operated (SO; n=7), orchidectomized (Orx; n=7) and daidzein treated orchidectomized (Orx+D; n=7) groups. Daidzein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Adrenocortical vascular network properties were identified using Novelli histochemical staining, vascular endothelial growth factor (VEGF) immunostaining and the newCast stereological software. Blood concentrations of aldosterone, corticosterone and DHEAS were measured by immunoassays. Volume densities of blood vessels and VEGF depots within the adrenal cortex were not significantly changed after daidzein treatment, although there was a tendency of decrease of these parameters. On the other hand, daidzein application increased (P<0.05) blood concentration of aldosterone by more than 2.5 times and 41%, in comparison with the same parameter in sham-operated and orchidectomized group, respectively. Corticosterone concentration in daidzein-treated, orchidectomized rats showed a tendency of elevation (P>0.05) compared to the both SO animals and orchidectomy alone. DHEAS blood concentration after daidzein application remained at the level of the Orx group, which in both cases ment elevation (P<0.05) by around 80% in comparison with the same parameter in SO group. We conclude that daidzein significantly increases aldosterone and to some lower extent corticosterone output in a rat model of the mild andropause, without significant rearrangements of the entire adrenal cortex vascular network.",
publisher = "bioscientifica",
journal = "25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey",
title = "Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause",
doi = "10.1530/endoabs.90.EP63",
pages = "394-395"
}

Čukuranović-Kokoris, J., Đorđević, M., Miler, M., Živanović, J., Marković Filipović, J., Stanković Đorević, D., Čukuranović, R., Milošević, V., Filipović, B.,& Ajdžanović, V.. (2023). Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause. in 25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey
bioscientifica., 394-395.
https://doi.org/10.1530/endoabs.90.EP63

Čukuranović-Kokoris J, Đorđević M, Miler M, Živanović J, Marković Filipović J, Stanković Đorević D, Čukuranović R, Milošević V, Filipović B, Ajdžanović V. Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause. in 25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey. 2023;:394-395.
doi:10.1530/endoabs.90.EP63 .

Čukuranović-Kokoris, Jovana, Đorđević, Miodrag, Miler, Marko, Živanović, Jasmina, Marković Filipović, Jelena, Stanković Đorević, Dobrila, Čukuranović, Rade, Milošević, Verica, Filipović, Branko, Ajdžanović, Vladimir, "Adrenocortical vascular network and hormonal output after daidzein application in a rat model of the mild andropause" in 25th European Congress of Endocrinology; 2023 Maz 13-16; Istanbul, Turkey (2023):394-395,
https://doi.org/10.1530/endoabs.90.EP63 . .

TY  - CONF
AU  - Marković Filipović, Jelena
AU  - Kojić, Danijela
AU  - Miler, Marko
AU  - Karan, Jelena
AU  - Ivelja, Ivana
AU  - Matavulj, Milica
PY  - 2022
UR  - https://www.hbd-sbc.hr/kongres2022/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6564
AB  - Acrylamide (AA) is carcinogen, mutagen and neurotoxic substance present in fried, roasted and
baked starch-based goods. AA is formed in Maillard reaction from asparagine and carbonyl
sources, such as reducing sugars, during thermal food processing at temperatures between
120°C and 180°C. The aim of our study was to determine whether acrylamide treatment affects
the expression and activity of glutathione-S-transferase (GST) in hepatocyte. Viability of rat
hepatoma cell line - H4IIE upon AA treatment was assessed by the MTT viability assay. Cells were
cultivated in a 96-well plate and exposed to increasing concentrations of AA (2.5–5.5 mM) for 24
h. AA concentration of 4 mM induced death of 17.62% H4IIE cells, while 4.5 mM AA caused death
of 48.45% cells. Therefore, AA concentrations of 4 mM and 4.5 mM were taken as IC20 and IC50,
respectively. H4IIE cells were treated with IC20 and IC50 of AA for 24 h. Relative mRNA
expression for GSTA2 and GSTP1 was quantified using real-time RT-PCR. Total GST activity was
determined spectrophotometrically at 340 nm using DTNB as the substrate. After AA treatments,
mRNA level for GSTA2 and GSTP1 and total GST activity increased in a concentration-dependent
manner in H4IIE cells. Only treatment with higher AA concentration (4.5 mM) proved to be
statistically significant for all three examined parameters. Our results indicate that by altering
expression and activity of GST enzyme acrylamide exerts toxic effects on hepatocytes.
PB  - Zagreb: Hrvatsko biološko društvo
C3  - Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska
T1  - Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes
SP  - 231
EP  - 232
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6564
ER  - 

@conference{
author = "Marković Filipović, Jelena and Kojić, Danijela and Miler, Marko and Karan, Jelena and Ivelja, Ivana and Matavulj, Milica",
year = "2022",
abstract = "Acrylamide (AA) is carcinogen, mutagen and neurotoxic substance present in fried, roasted and
baked starch-based goods. AA is formed in Maillard reaction from asparagine and carbonyl
sources, such as reducing sugars, during thermal food processing at temperatures between
120°C and 180°C. The aim of our study was to determine whether acrylamide treatment affects
the expression and activity of glutathione-S-transferase (GST) in hepatocyte. Viability of rat
hepatoma cell line - H4IIE upon AA treatment was assessed by the MTT viability assay. Cells were
cultivated in a 96-well plate and exposed to increasing concentrations of AA (2.5–5.5 mM) for 24
h. AA concentration of 4 mM induced death of 17.62% H4IIE cells, while 4.5 mM AA caused death
of 48.45% cells. Therefore, AA concentrations of 4 mM and 4.5 mM were taken as IC20 and IC50,
respectively. H4IIE cells were treated with IC20 and IC50 of AA for 24 h. Relative mRNA
expression for GSTA2 and GSTP1 was quantified using real-time RT-PCR. Total GST activity was
determined spectrophotometrically at 340 nm using DTNB as the substrate. After AA treatments,
mRNA level for GSTA2 and GSTP1 and total GST activity increased in a concentration-dependent
manner in H4IIE cells. Only treatment with higher AA concentration (4.5 mM) proved to be
statistically significant for all three examined parameters. Our results indicate that by altering
expression and activity of GST enzyme acrylamide exerts toxic effects on hepatocytes.",
publisher = "Zagreb: Hrvatsko biološko društvo",
journal = "Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska",
title = "Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes",
pages = "231-232",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6564"
}

Marković Filipović, J., Kojić, D., Miler, M., Karan, J., Ivelja, I.,& Matavulj, M.. (2022). Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes. in Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska
Zagreb: Hrvatsko biološko društvo., 231-232.
https://hdl.handle.net/21.15107/rcub_ibiss_6564

Marković Filipović J, Kojić D, Miler M, Karan J, Ivelja I, Matavulj M. Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes. in Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska. 2022;:231-232.
https://hdl.handle.net/21.15107/rcub_ibiss_6564 .

Marković Filipović, Jelena, Kojić, Danijela, Miler, Marko, Karan, Jelena, Ivelja, Ivana, Matavulj, Milica, "Effect of acrylamide treatment on the activity and expression of glutathione-S-transferase in rat hepatocytes" in Zbornik sažetaka: 14. Hrvatski biološki kongres s međunarodnim sudjelovanjem; 2022 Oct 12-16; Pula, Hrvatska (2022):231-232,
https://hdl.handle.net/21.15107/rcub_ibiss_6564 .

TY  - JOUR
AU  - Marković-Filipović, Jelena
AU  - Miler, Marko
AU  - Kojić, Danijela
AU  - Andrejić Višnjić, Bojana
AU  - Milošević, Verica
AU  - Cukuranovic Kokoris, Jovana
AU  - Đorđević, Miodrag
AU  - Matavulj, Milica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5480
AB  - Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory processes. In addition, AA is formed during processing of starchy food at high temperature. The aim of our study was to examine effects of subchronic AA treatment on adult rat liver using histological, stereological and biochemical methods. Adult male Wistar rats were treated with AA at doses of 25 mg/kg b.w. and 50 mg/kg b.w. for three weeks. Stereological analysis showed decrease of volume density of hepatocyte cytoplasm, and increase of volume density of hepatocyte nuclei and nucleocytoplasmic ratio in AA50mg group. Immunohistochemical analysis of the liver sections showed that treatment with AA50mg increase the percentage of PCNA positive cells, while the percentage of caspase 3 positive cells was not affected by AA. PAS-staining showed that glycogen content in hepatocytes was not affected by AA. Serological examination revealed increase of lipid peroxidation in AA50mg group, while total protein concentration, protein thiol group level, as well as, paraoxonase 1 activity were not changed in AA- exposed animals. Stereological and immunohistochemical analyses of adult liver sections suggest increase of proliferation in AA50mg group, while increase of lipid peroxidation in serum of AA50mg group indicates oxidative stress induction.
T2  - International Journal of Morphology
T1  - Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study
IS  - 6
VL  - 40
DO  - 10.4067/s0717-95022022000601618
SP  - 1618
EP  - 1623
ER  - 

@article{
author = "Marković-Filipović, Jelena and Miler, Marko and Kojić, Danijela and Andrejić Višnjić, Bojana and Milošević, Verica and Cukuranovic Kokoris, Jovana and Đorđević, Miodrag and Matavulj, Milica",
year = "2022",
abstract = "Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory processes. In addition, AA is formed during processing of starchy food at high temperature. The aim of our study was to examine effects of subchronic AA treatment on adult rat liver using histological, stereological and biochemical methods. Adult male Wistar rats were treated with AA at doses of 25 mg/kg b.w. and 50 mg/kg b.w. for three weeks. Stereological analysis showed decrease of volume density of hepatocyte cytoplasm, and increase of volume density of hepatocyte nuclei and nucleocytoplasmic ratio in AA50mg group. Immunohistochemical analysis of the liver sections showed that treatment with AA50mg increase the percentage of PCNA positive cells, while the percentage of caspase 3 positive cells was not affected by AA. PAS-staining showed that glycogen content in hepatocytes was not affected by AA. Serological examination revealed increase of lipid peroxidation in AA50mg group, while total protein concentration, protein thiol group level, as well as, paraoxonase 1 activity were not changed in AA- exposed animals. Stereological and immunohistochemical analyses of adult liver sections suggest increase of proliferation in AA50mg group, while increase of lipid peroxidation in serum of AA50mg group indicates oxidative stress induction.",
journal = "International Journal of Morphology",
title = "Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study",
number = "6",
volume = "40",
doi = "10.4067/s0717-95022022000601618",
pages = "1618-1623"
}

Marković-Filipović, J., Miler, M., Kojić, D., Andrejić Višnjić, B., Milošević, V., Cukuranovic Kokoris, J., Đorđević, M.,& Matavulj, M.. (2022). Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study. in International Journal of Morphology, 40(6), 1618-1623.
https://doi.org/10.4067/s0717-95022022000601618

Marković-Filipović J, Miler M, Kojić D, Andrejić Višnjić B, Milošević V, Cukuranovic Kokoris J, Đorđević M, Matavulj M. Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study. in International Journal of Morphology. 2022;40(6):1618-1623.
doi:10.4067/s0717-95022022000601618 .

Marković-Filipović, Jelena, Miler, Marko, Kojić, Danijela, Andrejić Višnjić, Bojana, Milošević, Verica, Cukuranovic Kokoris, Jovana, Đorđević, Miodrag, Matavulj, Milica, "Adult Rat Liver After Subchronic Acrylamide Treatment: Histological, Stereological and Biochemical Study" in International Journal of Morphology, 40, no. 6 (2022):1618-1623,
https://doi.org/10.4067/s0717-95022022000601618 . .

TY  - JOUR
AU  - Marković Filipović, Jelena
AU  - Miler, Marko
AU  - Kojić, Danijela
AU  - Karan, Jelena
AU  - Ivelja, Ivana
AU  - Čukuranović Kokoris, Jovana
AU  - Matavulj, Milica
PY  - 2022
UR  - https://www.mdpi.com/1422-0067/23/11/6062
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5007
AB  - Acrylamide (AA) toxicity is associated with oxidative stress. During detoxification, AA is either coupled to gluthatione or biotransformed to glycidamide by the enzyme cytochrome P450 2E1 (CYP2E1). The aim of our study was to examine the hepatotoxicity of AA in vivo and in vitro. Thirty male Wistar rats were treated with 25 or 50 mg/kg b.w. of AA for 3 weeks. Qualitative and quantitative immunohistochemical evaluation of inducible nitric oxide synthase (iNOS), CYP2E1, catalase (CAT), superoxide dismutase 1 (SOD1), and SOD2 expression in liver was carried out. Bearing in mind that the liver is consisted mainly of hepatocytes, in a parallel study, we used the rat hepatoma cell line H4IIE to investigate the effects of AA at IC20 and IC50 concentrations on the redox status and the activity of CAT, SOD, and glutathione-S-transferase (GST), their gene expression, and CYP2E1 and iNOS expression. Immunohistochemically stained liver sections showed that treatment with AA25mg induced a significant decrease of CYP2E1 protein expression (p < 0.05), while treatment with AA50mg led to a significant increase of iNOS protein expression (p < 0.05). AA treatment dose-dependently elevated SOD2 protein expression (p < 0.05), while SOD1 protein expression was significantly increased only at AA50mg (p < 0.05). CAT protein expression was not significantly affected by AA treatments (p > 0.05). In AA-treated H4IIE cells, a concentration-dependent significant increase in lipid peroxidation and nitrite levels was observed (p < 0.05), while GSH content and SOD activity significantly decreased in a concentration-dependent manner (p < 0.05). AA IC50 significantly enhanced GST activity (p < 0.05). The level of mRNA significantly increased in a concentration-dependent manner for iNOS, SOD2, and CAT in AA-treated H4IIE cells (p < 0.05). AA IC50 significantly increased the transcription of SOD1, GSTA2, and GSTP1 genes (p < 0.05), while AA IC20 significantly decreased mRNA for CYP2E1 in H4IIE cells (p < 0.05). Obtained results indicate that AA treatments, both in vivo and in vitro, change hepatocytes; drug-metabolizing potential and disturb its redox status.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes.
IS  - 11
VL  - 23
DO  - 10.3390/ijms23116062
SP  - 6062
ER  - 

@article{
author = "Marković Filipović, Jelena and Miler, Marko and Kojić, Danijela and Karan, Jelena and Ivelja, Ivana and Čukuranović Kokoris, Jovana and Matavulj, Milica",
year = "2022",
abstract = "Acrylamide (AA) toxicity is associated with oxidative stress. During detoxification, AA is either coupled to gluthatione or biotransformed to glycidamide by the enzyme cytochrome P450 2E1 (CYP2E1). The aim of our study was to examine the hepatotoxicity of AA in vivo and in vitro. Thirty male Wistar rats were treated with 25 or 50 mg/kg b.w. of AA for 3 weeks. Qualitative and quantitative immunohistochemical evaluation of inducible nitric oxide synthase (iNOS), CYP2E1, catalase (CAT), superoxide dismutase 1 (SOD1), and SOD2 expression in liver was carried out. Bearing in mind that the liver is consisted mainly of hepatocytes, in a parallel study, we used the rat hepatoma cell line H4IIE to investigate the effects of AA at IC20 and IC50 concentrations on the redox status and the activity of CAT, SOD, and glutathione-S-transferase (GST), their gene expression, and CYP2E1 and iNOS expression. Immunohistochemically stained liver sections showed that treatment with AA25mg induced a significant decrease of CYP2E1 protein expression (p < 0.05), while treatment with AA50mg led to a significant increase of iNOS protein expression (p < 0.05). AA treatment dose-dependently elevated SOD2 protein expression (p < 0.05), while SOD1 protein expression was significantly increased only at AA50mg (p < 0.05). CAT protein expression was not significantly affected by AA treatments (p > 0.05). In AA-treated H4IIE cells, a concentration-dependent significant increase in lipid peroxidation and nitrite levels was observed (p < 0.05), while GSH content and SOD activity significantly decreased in a concentration-dependent manner (p < 0.05). AA IC50 significantly enhanced GST activity (p < 0.05). The level of mRNA significantly increased in a concentration-dependent manner for iNOS, SOD2, and CAT in AA-treated H4IIE cells (p < 0.05). AA IC50 significantly increased the transcription of SOD1, GSTA2, and GSTP1 genes (p < 0.05), while AA IC20 significantly decreased mRNA for CYP2E1 in H4IIE cells (p < 0.05). Obtained results indicate that AA treatments, both in vivo and in vitro, change hepatocytes; drug-metabolizing potential and disturb its redox status.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes.",
number = "11",
volume = "23",
doi = "10.3390/ijms23116062",
pages = "6062"
}

Marković Filipović, J., Miler, M., Kojić, D., Karan, J., Ivelja, I., Čukuranović Kokoris, J.,& Matavulj, M.. (2022). Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes.. in International Journal of Molecular Sciences
Basel: MDPI., 23(11), 6062.
https://doi.org/10.3390/ijms23116062

Marković Filipović J, Miler M, Kojić D, Karan J, Ivelja I, Čukuranović Kokoris J, Matavulj M. Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes.. in International Journal of Molecular Sciences. 2022;23(11):6062.
doi:10.3390/ijms23116062 .

Marković Filipović, Jelena, Miler, Marko, Kojić, Danijela, Karan, Jelena, Ivelja, Ivana, Čukuranović Kokoris, Jovana, Matavulj, Milica, "Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes." in International Journal of Molecular Sciences, 23, no. 11 (2022):6062,
https://doi.org/10.3390/ijms23116062 . .

TY  - JOUR
AU  - Čukuranović-Kokoris, Jovana
AU  - Đorđević, Miodrag
AU  - Jovanović, Ivan
AU  - Kundalić, Braca
AU  - Pavlović, Miljana
AU  - Graovac, Ivana
AU  - Ajdžanović, Vladimir
AU  - Ristić, Nataša
AU  - Marković-Filipović, Jelena
AU  - Milošević, Verica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4978
AB  - Introduction/Objective. In this study, we have pointed out the immunohistomorphometric characteristics of somatotropic (GH) and folliculo-stellate (FS) cells of the human pituitary gland during ageing. Methods. On histological sections of the pituitary gland of 14 male cadavers of different ages, the GH and FS cells were immunohistochemically labeled with corresponding antibodies, monoclonal anti-GH antibody and polyclonal anti-S100 antibody, respectively. Immunopositive GH- and FS-cells were further morphometrically analyzed using ImageJ software. Results. The obtained results of morphometric analysis showed that the surface area of GH cells increased significantly with age. In these cells, the nuclear-cytoplasmic ratio gradually decreased and became significantly higher after the age of 70 years. The volume density of GH cells has not changed during ageing, while in FS cells this parameter significantly increased in the cases older than 70 years. The nuclear-cytoplasmic ratio of GH cells is negatively correlated with the volume density of FS cells. Conclusions. Based on the obtained results, we concluded that hypertrophy of GH and FS cells occurs in men with ageing and that correlation between the morphometric parameters of these two cell types indicates their mutual interaction.
AB  - Увод/Циљ У овој студији смо указали на имунохистоморфометријске карактеристике соматотропних (ГХ) и фоликулостелатних (ФС) ћелија хипофизе човека током старења. Методе На хистолошким пресецима хипофизе 14 мушких кадавера различите старости, ГХ и ФС ћелије су имунохистохемијски обележене одговарајућим антителима (моноклонско анти-ГХ антитело и поликлонско анти-С100 антитело). Имунопозитивне ГХ- и ФС-ћелије су морфометријски анализиране коришћењем ImageJ софтвера. Резултати Добијени резултати морфометријске анализе су показали да се површина коју заузимају ГХ ћелије значајно повећава са старењем. У овим ћелијама нуклеарно-цитоплазматски однос се постепено смањивао и након 70. године старости постао значајно већи. Волуменска густина ГХ ћелија се није мењала током старења, док је код ФС ћелија овај параметар значајно повећан у случајевима старијим од 70 година. Нуклеарно-цитоплазматски однос ГХ ћелија је у негативној корелацији са волуменском густином ФС ћелија. Закључак На основу добијених резултата, закључили смо да се хипертрофија ГХ и ФС ћелија јавља код мушкараца са старењем и да корелација између морфометријских параметара ова два типа ћелија указује на њихову међусобну интеракцију.
PB  - Belgrade: Serbian Medical Society
T2  - Srpski arhiv za celokupno lekarstvo - Serbian Archives of Medicine
T1  - Morphometric analysis of somatotropic and folliculostellate cells of human anterior pituitary during ageing
T1  - Морфометријска анализа соматотропних и фоликулостелатних ћелија
аденохипофизе човека током старења
IS  - 5-6
VL  - 150
DO  - 10.2298/SARH211214044C
SP  - 274
EP  - 280
ER  - 

@article{
author = "Čukuranović-Kokoris, Jovana and Đorđević, Miodrag and Jovanović, Ivan and Kundalić, Braca and Pavlović, Miljana and Graovac, Ivana and Ajdžanović, Vladimir and Ristić, Nataša and Marković-Filipović, Jelena and Milošević, Verica",
year = "2022",
abstract = "Introduction/Objective. In this study, we have pointed out the immunohistomorphometric characteristics of somatotropic (GH) and folliculo-stellate (FS) cells of the human pituitary gland during ageing. Methods. On histological sections of the pituitary gland of 14 male cadavers of different ages, the GH and FS cells were immunohistochemically labeled with corresponding antibodies, monoclonal anti-GH antibody and polyclonal anti-S100 antibody, respectively. Immunopositive GH- and FS-cells were further morphometrically analyzed using ImageJ software. Results. The obtained results of morphometric analysis showed that the surface area of GH cells increased significantly with age. In these cells, the nuclear-cytoplasmic ratio gradually decreased and became significantly higher after the age of 70 years. The volume density of GH cells has not changed during ageing, while in FS cells this parameter significantly increased in the cases older than 70 years. The nuclear-cytoplasmic ratio of GH cells is negatively correlated with the volume density of FS cells. Conclusions. Based on the obtained results, we concluded that hypertrophy of GH and FS cells occurs in men with ageing and that correlation between the morphometric parameters of these two cell types indicates their mutual interaction., Увод/Циљ У овој студији смо указали на имунохистоморфометријске карактеристике соматотропних (ГХ) и фоликулостелатних (ФС) ћелија хипофизе човека током старења. Методе На хистолошким пресецима хипофизе 14 мушких кадавера различите старости, ГХ и ФС ћелије су имунохистохемијски обележене одговарајућим антителима (моноклонско анти-ГХ антитело и поликлонско анти-С100 антитело). Имунопозитивне ГХ- и ФС-ћелије су морфометријски анализиране коришћењем ImageJ софтвера. Резултати Добијени резултати морфометријске анализе су показали да се површина коју заузимају ГХ ћелије значајно повећава са старењем. У овим ћелијама нуклеарно-цитоплазматски однос се постепено смањивао и након 70. године старости постао значајно већи. Волуменска густина ГХ ћелија се није мењала током старења, док је код ФС ћелија овај параметар значајно повећан у случајевима старијим од 70 година. Нуклеарно-цитоплазматски однос ГХ ћелија је у негативној корелацији са волуменском густином ФС ћелија. Закључак На основу добијених резултата, закључили смо да се хипертрофија ГХ и ФС ћелија јавља код мушкараца са старењем и да корелација између морфометријских параметара ова два типа ћелија указује на њихову међусобну интеракцију.",
publisher = "Belgrade: Serbian Medical Society",
journal = "Srpski arhiv za celokupno lekarstvo - Serbian Archives of Medicine",
title = "Morphometric analysis of somatotropic and folliculostellate cells of human anterior pituitary during ageing, Морфометријска анализа соматотропних и фоликулостелатних ћелија
аденохипофизе човека током старења",
number = "5-6",
volume = "150",
doi = "10.2298/SARH211214044C",
pages = "274-280"
}

Čukuranović-Kokoris, J., Đorđević, M., Jovanović, I., Kundalić, B., Pavlović, M., Graovac, I., Ajdžanović, V., Ristić, N., Marković-Filipović, J.,& Milošević, V.. (2022). Morphometric analysis of somatotropic and folliculostellate cells of human anterior pituitary during ageing. in Srpski arhiv za celokupno lekarstvo - Serbian Archives of Medicine
Belgrade: Serbian Medical Society., 150(5-6), 274-280.
https://doi.org/10.2298/SARH211214044C

Čukuranović-Kokoris J, Đorđević M, Jovanović I, Kundalić B, Pavlović M, Graovac I, Ajdžanović V, Ristić N, Marković-Filipović J, Milošević V. Morphometric analysis of somatotropic and folliculostellate cells of human anterior pituitary during ageing. in Srpski arhiv za celokupno lekarstvo - Serbian Archives of Medicine. 2022;150(5-6):274-280.
doi:10.2298/SARH211214044C .

Čukuranović-Kokoris, Jovana, Đorđević, Miodrag, Jovanović, Ivan, Kundalić, Braca, Pavlović, Miljana, Graovac, Ivana, Ajdžanović, Vladimir, Ristić, Nataša, Marković-Filipović, Jelena, Milošević, Verica, "Morphometric analysis of somatotropic and folliculostellate cells of human anterior pituitary during ageing" in Srpski arhiv za celokupno lekarstvo - Serbian Archives of Medicine, 150, no. 5-6 (2022):274-280,
https://doi.org/10.2298/SARH211214044C . .

TY  - CONF
AU  - Marković Filipović, Jelena
AU  - Ivelja, Ivana
AU  - Karan, Jelena
AU  - Miler, Marko
AU  - Matavulj, Milica
PY  - 2022
UR  - https://www.serbiosoc.org.rs/?page_id=702
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6036
AB  - Akrilamid (AA) je toksična industrijska hemikalija čiji se metabolizam odvija u jetri. Dodatno, AA se nalazi u često konzumiranoj hrani bogatoj ugljenim hidratima koja se sprema na visokim temperaturama. Cilj naše studije je da ispitamo efekat AA na proliferativne i apoptotičke karakteristike jetre odraslih pacova, koristeći imunohistohemijske (IHC) metode. Odrasli mužjaci pacova soja Wistar, stari 65 dana tretirani su dozama AA od 25 mg/kg i 50 mg/kg. Tkivo prethodno fiksirano u formalinu i ukalupljeno u parafin, isečeno je na preseke debljine 5 µm i obojeno imunohistohemijski koristeći anti-PCNA i anticaspase 3 antitela. Procenat obojene površine imunohistohemijski obojenih preseka određen je pomoću ImageJ dodatka – IHC profiler. U kontrolnoj grupi i grupama tretiranim akrilamidom ekspresija PCNA primećena je u jedrima hepatocita. AA tretman dovodi do povećanja brojnosti PCNA pozitivnih ćelija koje je zavisno od doze. Povećanje brojnosti PCNA pozitivnih ćelija pokazalo je i statističku značajnost u grupi tretiranoj sa AA50mg. U svim grupama, imunobojenje kaspazom 3 pokazalo je slabu citoplazmatsku imunoreaktivnost. Statistička analiza ukazala je na to da AA tretman nije značajno uticao na procenat ćelija pozitivnih na kaspazu 3. Imunohistohemijska analiza preseka jetre pokazala je da AA50mg tretman utiče na proliferativnost, ali ne i apoptozu hepatocita pacova.
AB  - Акриламид (АА) је токсична индустријска хемикалија чији се метаболизам одвија
у јетри. Додатно, АА се налази у често конзумираној храни богатој угљеним
хидратима која се спрема на високим температурама. Циљ наше студије је да
испитамо ефекат АА на пролиферативне и апоптотичке карактеристике јетре
одраслих пацова, користећи имунохистохемијске (ИХЦ) методе. Одрасли мужјаци
пацова соја Wistar, стари 65 дана третирани су дозама АА од 25 мг/кг и 50 мг/кг.
Ткиво претходно фиксирано у формалину и укалупљено у парафин, исечено је на
пресеке дебљине 5 µm и обојено имунохистохемијски користећи анти-PCNA и anticaspase 3 антитела. Проценат обојене површине имунохистохемијски обојених
пресека одређен је помоћу ImageJ додатка – IHC profiler. У контролној групи и
групама третираним акриламидом експресија PCNA примећена је у једрима
хепатоцита. АА третман доводи до повећања бројности PCNA позитивних ћелија
које је зависно од дозе. Повећање бројности PCNA позитивних ћелија показало је и
статистичку значајност у групи третираној са AA50мг. У свим групама, имунобојење
каспазом 3 показало је слабу цитоплазматску имунореактивност. Статистичка
анализа указала је на то да АА третман није значајно утицао на проценат ћелија
позитивних на каспазу 3. Имунохистохемијска анализа пресека јетре показала је да
AA50мг третман утиче на пролиферативност, али не и апоптозу хепатоцита пацова.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova
T1  - Ефекат третмана акриламидом на пролиферативне и апоптотичке карактеристике јетре одраслих пацова
SP  - 366
EP  - 366
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6036
ER  - 

@conference{
author = "Marković Filipović, Jelena and Ivelja, Ivana and Karan, Jelena and Miler, Marko and Matavulj, Milica",
year = "2022",
abstract = "Akrilamid (AA) je toksična industrijska hemikalija čiji se metabolizam odvija u jetri. Dodatno, AA se nalazi u često konzumiranoj hrani bogatoj ugljenim hidratima koja se sprema na visokim temperaturama. Cilj naše studije je da ispitamo efekat AA na proliferativne i apoptotičke karakteristike jetre odraslih pacova, koristeći imunohistohemijske (IHC) metode. Odrasli mužjaci pacova soja Wistar, stari 65 dana tretirani su dozama AA od 25 mg/kg i 50 mg/kg. Tkivo prethodno fiksirano u formalinu i ukalupljeno u parafin, isečeno je na preseke debljine 5 µm i obojeno imunohistohemijski koristeći anti-PCNA i anticaspase 3 antitela. Procenat obojene površine imunohistohemijski obojenih preseka određen je pomoću ImageJ dodatka – IHC profiler. U kontrolnoj grupi i grupama tretiranim akrilamidom ekspresija PCNA primećena je u jedrima hepatocita. AA tretman dovodi do povećanja brojnosti PCNA pozitivnih ćelija koje je zavisno od doze. Povećanje brojnosti PCNA pozitivnih ćelija pokazalo je i statističku značajnost u grupi tretiranoj sa AA50mg. U svim grupama, imunobojenje kaspazom 3 pokazalo je slabu citoplazmatsku imunoreaktivnost. Statistička analiza ukazala je na to da AA tretman nije značajno uticao na procenat ćelija pozitivnih na kaspazu 3. Imunohistohemijska analiza preseka jetre pokazala je da AA50mg tretman utiče na proliferativnost, ali ne i apoptozu hepatocita pacova., Акриламид (АА) је токсична индустријска хемикалија чији се метаболизам одвија
у јетри. Додатно, АА се налази у често конзумираној храни богатој угљеним
хидратима која се спрема на високим температурама. Циљ наше студије је да
испитамо ефекат АА на пролиферативне и апоптотичке карактеристике јетре
одраслих пацова, користећи имунохистохемијске (ИХЦ) методе. Одрасли мужјаци
пацова соја Wistar, стари 65 дана третирани су дозама АА од 25 мг/кг и 50 мг/кг.
Ткиво претходно фиксирано у формалину и укалупљено у парафин, исечено је на
пресеке дебљине 5 µm и обојено имунохистохемијски користећи анти-PCNA и anticaspase 3 антитела. Проценат обојене површине имунохистохемијски обојених
пресека одређен је помоћу ImageJ додатка – IHC profiler. У контролној групи и
групама третираним акриламидом експресија PCNA примећена је у једрима
хепатоцита. АА третман доводи до повећања бројности PCNA позитивних ћелија
које је зависно од дозе. Повећање бројности PCNA позитивних ћелија показало је и
статистичку значајност у групи третираној са AA50мг. У свим групама, имунобојење
каспазом 3 показало је слабу цитоплазматску имунореактивност. Статистичка
анализа указала је на то да АА третман није значајно утицао на проценат ћелија
позитивних на каспазу 3. Имунохистохемијска анализа пресека јетре показала је да
AA50мг третман утиче на пролиферативност, али не и апоптозу хепатоцита пацова.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova, Ефекат третмана акриламидом на пролиферативне и апоптотичке карактеристике јетре одраслих пацова",
pages = "366-366",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6036"
}

Marković Filipović, J., Ivelja, I., Karan, J., Miler, M.,& Matavulj, M.. (2022). Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 366-366.
https://hdl.handle.net/21.15107/rcub_ibiss_6036

Marković Filipović J, Ivelja I, Karan J, Miler M, Matavulj M. Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:366-366.
https://hdl.handle.net/21.15107/rcub_ibiss_6036 .

Marković Filipović, Jelena, Ivelja, Ivana, Karan, Jelena, Miler, Marko, Matavulj, Milica, "Efekat tretmana akrilamidom na proliferativne i apoptotičke karakteristike jetre odraslih pacova" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):366-366,
https://hdl.handle.net/21.15107/rcub_ibiss_6036 .

TY  - CONF
AU  - Marković Filipović, Jelena
AU  - Ivelja, Ivana
AU  - Karan, Jelena
AU  - Miler, Marko
AU  - Milošević, Verica
AU  - Matavulj, Milica
PY  - 2021
UR  - https://ibsc2021.pmf.uns.ac.rs/
UR  - https://ibsc2021.pmf.uns.ac.rs/ebook-of-abstracts/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6577
AB  - INTRODUCTION: Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory purposes. In addition, AA is formed in many types of fried and oven-baked foods during cooking. Considering proven neurotoxic, carcinogenic and mutagenic effects on living organisms, AA became a main topic of interest for many research groups. OBJECTIVES: The objective of our study was to determine whether acrylamide treatment affects superoxide dismutase 1 (SOD1) expression in rat liver. METHOD / DESIGN: Adult male Wistar rats were subchronicly (three weeks) treated with 25 mg/kg or 50 mg/kg body weight (b.w.) of AA. Formalin-fixed paraffin-embedded liver tissue was cut into 5 µm thin sections and immunostained with anti-SOD1 antibody. The amount of SOD1 in immunostained sections was determined using Windows based ImageJ program (ImageJ, Version 1.50f). The optical density (OD) and stained percentage color area of immunolabeled SOD1 were measured. RESULTS: Figure 1. Representative micrographs of superoxide dismutase 1 (SOD1) immunohistochemical staining in liver of control rats, rats treated with
acrylamide (AA) in dose of 25 mg/kg b.w., and rats treated with acrylamide in dose of 50 mg/kg b.w. (a). Optical density of SOD1 immunopositive cells in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (b). Percentage contribution of high positive, positive, low positive and total positive immunohistochemical staining of SOD1 in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (c). Values in charts are means ± SEM; n = 10, *p < 0.05. In statistical analysis AA-treated animals were compared with the control group. CONCLUSIONS: Immunostaining of SOD1 in liver of control rats showed weak cytoplasmic immunoreactivity in hepatocytes (Fig. 1a). AA application induced dose dependent increase of immunostaining intensity (Fig. 1a). Significant increase of OD and percentage contribution of low positive and total positive cells of immunostained SOD1 was detected in group treated with AA in a dose of 50 mg/kg (Fig.1b, c).
PB  - Novi Sad: Faculty of Sciences, University of Novi Sad
C3  - Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
T1  - Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver
SP  - 137
EP  - 138
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6577
ER  - 

@conference{
author = "Marković Filipović, Jelena and Ivelja, Ivana and Karan, Jelena and Miler, Marko and Milošević, Verica and Matavulj, Milica",
year = "2021",
abstract = "INTRODUCTION: Acrylamide (AA) is a widely used chemical and an important monomer in various industrial and laboratory purposes. In addition, AA is formed in many types of fried and oven-baked foods during cooking. Considering proven neurotoxic, carcinogenic and mutagenic effects on living organisms, AA became a main topic of interest for many research groups. OBJECTIVES: The objective of our study was to determine whether acrylamide treatment affects superoxide dismutase 1 (SOD1) expression in rat liver. METHOD / DESIGN: Adult male Wistar rats were subchronicly (three weeks) treated with 25 mg/kg or 50 mg/kg body weight (b.w.) of AA. Formalin-fixed paraffin-embedded liver tissue was cut into 5 µm thin sections and immunostained with anti-SOD1 antibody. The amount of SOD1 in immunostained sections was determined using Windows based ImageJ program (ImageJ, Version 1.50f). The optical density (OD) and stained percentage color area of immunolabeled SOD1 were measured. RESULTS: Figure 1. Representative micrographs of superoxide dismutase 1 (SOD1) immunohistochemical staining in liver of control rats, rats treated with
acrylamide (AA) in dose of 25 mg/kg b.w., and rats treated with acrylamide in dose of 50 mg/kg b.w. (a). Optical density of SOD1 immunopositive cells in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (b). Percentage contribution of high positive, positive, low positive and total positive immunohistochemical staining of SOD1 in control and AA-treated rats in doses of 25 mg/kg b.w. and 50 mg/kg b.w. (c). Values in charts are means ± SEM; n = 10, *p < 0.05. In statistical analysis AA-treated animals were compared with the control group. CONCLUSIONS: Immunostaining of SOD1 in liver of control rats showed weak cytoplasmic immunoreactivity in hepatocytes (Fig. 1a). AA application induced dose dependent increase of immunostaining intensity (Fig. 1a). Significant increase of OD and percentage contribution of low positive and total positive cells of immunostained SOD1 was detected in group treated with AA in a dose of 50 mg/kg (Fig.1b, c).",
publisher = "Novi Sad: Faculty of Sciences, University of Novi Sad",
journal = "Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia",
title = "Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver",
pages = "137-138",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6577"
}

Marković Filipović, J., Ivelja, I., Karan, J., Miler, M., Milošević, V.,& Matavulj, M.. (2021). Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
Novi Sad: Faculty of Sciences, University of Novi Sad., 137-138.
https://hdl.handle.net/21.15107/rcub_ibiss_6577

Marković Filipović J, Ivelja I, Karan J, Miler M, Milošević V, Matavulj M. Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia. 2021;:137-138.
https://hdl.handle.net/21.15107/rcub_ibiss_6577 .

Marković Filipović, Jelena, Ivelja, Ivana, Karan, Jelena, Miler, Marko, Milošević, Verica, Matavulj, Milica, "Subchronic acrylamide treatment induces superoxide dismutase 1 expression in rat liver" in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia (2021):137-138,
https://hdl.handle.net/21.15107/rcub_ibiss_6577 .

TY  - CONF
AU  - Marković Filipović, Jelena
AU  - Kojić, Danijela
AU  - Miler, Marko
AU  - Milošević, Verica
AU  - Matavulj, Milica
PY  - 2021
UR  - https://ibsc2021.pmf.uns.ac.rs/
UR  - https://ibsc2021.pmf.uns.ac.rs/ebook-of-abstracts/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6578
AB  - INTRODUCTION: Acrylamide (AA) is industrial toxic substance with neurotoxic and reprotoxic effects. AA is a Maillard reaction product formed during processing of starchy food at high temperature.
OBJECTIVES: The objective of our study was to determine whether acrylamide treatment disturbs redox balance by altering nitrite, gluthatione (GSH), and malondialdehyde levels in rat hepatoma cell line (H4IIE). METHOD / DESIGN:
Rat hepatoma cell line H4IIE was treated with 4 mM (IC20) and 4.5 mM (IC50) of AA for 24 h. The nitrite level in the medium was analyzed as an indicator of NO production following the Griess reaction method. After ultrasonic cell lysis in 2.5% sulfocalicylic acid, supernatant was analysed for the content of gluthatione. Lipid peroxidation was evaluated using thiobarbituric acid reactive substance assay (TBARS). RESULTS: Detected nitrite, malondialdehyde and GSH levels in rat hepatoma cell line H4IIE after acrylamide treatment are shown in Figure 1. Figure 1. Nitrite concentration (a), malondialdehyde (MDA) concentration (b), reduced glutathione (GSH) concentration (c) in H4IIE cells after treatment with 4 and 4.5 mM acrylamide (AA) for 24 h. Values in charts are means ± SEM of three experiments performed in triplicate. Mean values were significantly different from that of untreated control cells (*p<0.05). CONCLUSIONS: In rat hepatoma cell line H4IIE, exposure to AA caused significant concentration-dependent increase of nitrite level and lipid peroxidation (Fig. 1a, b). On the other hand, GSH content significantly decreased in a concentration-dependent manner in H4IIE cells (Fig.1c). Obtained results indicate that AA disturbs redox status in hepatocytes.
PB  - Novi Sad: Faculty of Sciences, University of Novi Sad
C3  - Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
T1  - Acrylamide treatment affects oxidative stress parameters in rat hepatocytes
SP  - 139
EP  - 140
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6578
ER  - 

@conference{
author = "Marković Filipović, Jelena and Kojić, Danijela and Miler, Marko and Milošević, Verica and Matavulj, Milica",
year = "2021",
abstract = "INTRODUCTION: Acrylamide (AA) is industrial toxic substance with neurotoxic and reprotoxic effects. AA is a Maillard reaction product formed during processing of starchy food at high temperature.
OBJECTIVES: The objective of our study was to determine whether acrylamide treatment disturbs redox balance by altering nitrite, gluthatione (GSH), and malondialdehyde levels in rat hepatoma cell line (H4IIE). METHOD / DESIGN:
Rat hepatoma cell line H4IIE was treated with 4 mM (IC20) and 4.5 mM (IC50) of AA for 24 h. The nitrite level in the medium was analyzed as an indicator of NO production following the Griess reaction method. After ultrasonic cell lysis in 2.5% sulfocalicylic acid, supernatant was analysed for the content of gluthatione. Lipid peroxidation was evaluated using thiobarbituric acid reactive substance assay (TBARS). RESULTS: Detected nitrite, malondialdehyde and GSH levels in rat hepatoma cell line H4IIE after acrylamide treatment are shown in Figure 1. Figure 1. Nitrite concentration (a), malondialdehyde (MDA) concentration (b), reduced glutathione (GSH) concentration (c) in H4IIE cells after treatment with 4 and 4.5 mM acrylamide (AA) for 24 h. Values in charts are means ± SEM of three experiments performed in triplicate. Mean values were significantly different from that of untreated control cells (*p<0.05). CONCLUSIONS: In rat hepatoma cell line H4IIE, exposure to AA caused significant concentration-dependent increase of nitrite level and lipid peroxidation (Fig. 1a, b). On the other hand, GSH content significantly decreased in a concentration-dependent manner in H4IIE cells (Fig.1c). Obtained results indicate that AA disturbs redox status in hepatocytes.",
publisher = "Novi Sad: Faculty of Sciences, University of Novi Sad",
journal = "Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia",
title = "Acrylamide treatment affects oxidative stress parameters in rat hepatocytes",
pages = "139-140",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6578"
}

Marković Filipović, J., Kojić, D., Miler, M., Milošević, V.,& Matavulj, M.. (2021). Acrylamide treatment affects oxidative stress parameters in rat hepatocytes. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia
Novi Sad: Faculty of Sciences, University of Novi Sad., 139-140.
https://hdl.handle.net/21.15107/rcub_ibiss_6578

Marković Filipović J, Kojić D, Miler M, Milošević V, Matavulj M. Acrylamide treatment affects oxidative stress parameters in rat hepatocytes. in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia. 2021;:139-140.
https://hdl.handle.net/21.15107/rcub_ibiss_6578 .

Marković Filipović, Jelena, Kojić, Danijela, Miler, Marko, Milošević, Verica, Matavulj, Milica, "Acrylamide treatment affects oxidative stress parameters in rat hepatocytes" in Book of abstracts: The International Bioscience Conference and the 8th International PSU - UNS Bioscience Conference IBSC 2021; 2021 Nov 25-26; Novi Sad, Serbia (2021):139-140,
https://hdl.handle.net/21.15107/rcub_ibiss_6578 .

TY  - JOUR
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Marković Filipović, Jelena
AU  - Šošić-Jurjević, Branka 
AU  - Milošević, Verica
PY  - 2021
UR  - https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/abs/thyroid-gland-alterations-in-oldaged-wistar-rats-a-comprehensive-stereological-ultrastructural-hormonal-and-gene-expression-study/4C35B9A6AFED404C65272C08C8C2DC27
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4143
AB  - The aim of the present study was to determine and elaborate on all changes in old-aged (OA) versus young-aged (YA) rat thyroids by using
stereological, ultrastructural, hormonal, and gene expression analyses. We used 4- and 24-month-old male Wistar rats in our evaluation,
presenting all changes in comparison with YA rats. Results showed that the thyroid parenchyma was characterized by higher absolute
volumes of the gland, colloid, epithelium, and interstitium by 135, 135, 140, and 142% ( p < 0.05) respectively, while the relative volumes
of colloid and glands were unchanged. Ultrastructural analysis revealed less active glands, with smaller amounts of lysosomes, thyroglobulin
(Tg) granules, and microvilli in the luminal colloid. Optical density values for thyroid peroxidase (TPO), Tg, and vascular-endothelial
growth factor immunostaining remained unchanged; however, TPO and Tg exhibited visually stronger expression in small active follicles.
Thyroxine (T4)-Tg, the relative intensity of fluorescence (RIF), serum T4, and the sodium-iodide symporter immunohistochemical and gene
expressions decreased by 20, 40, 29, and 31% ( p < 0.05), respectively, in OA thyroids. Pituitary thyroid-stimulating hormone (TSH) RIF
increased by 44% ( p < 0.05), but the TSH serum concentration remained unchanged. In conclusion, the obtained results indicate depression
of the thyroid gland synthetic and secretory capacity with advanced age.
PB  - Cambridge University Press
T2  - Microscopy and Microanalysis
T1  - Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study
DO  - 10.1017/S1431927621000064
ER  - 

@article{
author = "Miler, Marko and Ajdžanović, Vladimir and Živanović, Jasmina and Marković Filipović, Jelena and Šošić-Jurjević, Branka  and Milošević, Verica",
year = "2021",
abstract = "The aim of the present study was to determine and elaborate on all changes in old-aged (OA) versus young-aged (YA) rat thyroids by using
stereological, ultrastructural, hormonal, and gene expression analyses. We used 4- and 24-month-old male Wistar rats in our evaluation,
presenting all changes in comparison with YA rats. Results showed that the thyroid parenchyma was characterized by higher absolute
volumes of the gland, colloid, epithelium, and interstitium by 135, 135, 140, and 142% ( p < 0.05) respectively, while the relative volumes
of colloid and glands were unchanged. Ultrastructural analysis revealed less active glands, with smaller amounts of lysosomes, thyroglobulin
(Tg) granules, and microvilli in the luminal colloid. Optical density values for thyroid peroxidase (TPO), Tg, and vascular-endothelial
growth factor immunostaining remained unchanged; however, TPO and Tg exhibited visually stronger expression in small active follicles.
Thyroxine (T4)-Tg, the relative intensity of fluorescence (RIF), serum T4, and the sodium-iodide symporter immunohistochemical and gene
expressions decreased by 20, 40, 29, and 31% ( p < 0.05), respectively, in OA thyroids. Pituitary thyroid-stimulating hormone (TSH) RIF
increased by 44% ( p < 0.05), but the TSH serum concentration remained unchanged. In conclusion, the obtained results indicate depression
of the thyroid gland synthetic and secretory capacity with advanced age.",
publisher = "Cambridge University Press",
journal = "Microscopy and Microanalysis",
title = "Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study",
doi = "10.1017/S1431927621000064"
}

Miler, M., Ajdžanović, V., Živanović, J., Marković Filipović, J., Šošić-Jurjević, B.,& Milošević, V.. (2021). Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study. in Microscopy and Microanalysis
Cambridge University Press..
https://doi.org/10.1017/S1431927621000064

Miler M, Ajdžanović V, Živanović J, Marković Filipović J, Šošić-Jurjević B, Milošević V. Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study. in Microscopy and Microanalysis. 2021;.
doi:10.1017/S1431927621000064 .

Miler, Marko, Ajdžanović, Vladimir, Živanović, Jasmina, Marković Filipović, Jelena, Šošić-Jurjević, Branka , Milošević, Verica, "Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study" in Microscopy and Microanalysis (2021),
https://doi.org/10.1017/S1431927621000064 . .